KR20070049788A - Cosmetic composition comprising the cortex extract of colocasia antiquorum having inhibiting activity of melanin synthesis - Google Patents

Abstract

Since the extract of the present invention (cortex of Colocasia antiquorum ) significantly inhibits melanin production by inhibiting tyrosinase involved in melanin biosynthesis, the composition comprising the same can be usefully used as a cosmetic composition for whitening .

Taro, whitening, melanin, tyrosinase, cosmetic composition

Description

 Cosmetic composition comprising the cortex extract of Colocasia antiquorum having inhibiting activity of melanin synthesis}

 1 is a diagram showing the effect of the concentration of tarophyl extract on melanin production and cell viability for melanocytes.

The present invention relates to a cosmetic composition for whitening containing the extract of Cortex of Colocasia antiquorum as a whitening active ingredient.

Melanogenesis is a defense mechanism against melanocyte pigmentation cells (melanocytes) in the skin cells, which increases the melanogenesis activity, and the large amount of melanin produced by this transfers to the keratinocytes. It is the result of accumulation in the epidermis. Although melanin protects the skin, hyperpigmentation of the skin causes blemishes, freckles, darkening of the skin after skin inflammation, and senile pigment spots. It may also result. Therefore, recently, various whitening cosmetics and medicines for preventing and improving such problems have been developed and marketed.

The research of whitening agent as cosmetics has recently been increasingly needed as skin blackening is recognized as skin aging caused by ultraviolet rays along with the improvement of living standard in the Asian region which prefers emotionally white skin. Since 1990, arbutin, kojic acid, vitamin C, and derivatives thereof have been developed, and whitening cosmetics containing them are commercially available, but the actual clinical effect is not satisfactory.

On the other hand, in order to develop a whitening cosmetics having relatively low toxicity and nature-friendly, researches are being conducted to search for and use herbal medicines with whitening effects. Through these studies, several herbal medicines have been derived. For example, Korean Patent Publication No. 1991-8660 discloses whitening cosmetics containing extract of Dacchi, and Korean Patent Publication No. 1991-11645 contains Hojang-geun extract. A whitening cosmetic has been disclosed, and Korean Patent Publication No. 1993-28783 discloses a whitening composition containing an extract of lettuce, and more recently, a whitening cosmetic containing melberin isolated from mulberry trees is disclosed in Korean Patent Publication No. 1997. Published in -47260.

 In the development of the whitening agent, the main part of the oxidation inhibitory function of tyrosine by tyrosinase, the first step of the melanin pigmentation process, is considered as a main focus. Therefore, screening tyrosinase activity inhibitors in vitro is an important whitening agent development tool. Arbutin, kojic acid, vitamin C, etc., which are currently used, are also derived from tyrosinase inhibitors and are widely used.

Therefore, in the present invention, based on the tyrosinase activity inhibitory effect at the same time by measuring the cytotoxicity and melanogenesis inhibitory using melan-A (melan-A) cell line to compensate for the above problems by using a novel new functional whitening agent Screening was done.

The inventors of the present invention, while searching for plants that are expected to have excellent whitening activity from natural resources existing in nature, found that taro inhibits the activity of tyrosinase, a whitening-related enzyme, and inhibits the production of melanin pigment. Discovered and completed the present invention.

Taro ( Colocasia antiquorum ), also known as a cultivation, is native to tropical Asia, such as India and Indonesia, and is a widely grown vegetable. Taro contains a lot of sugars, most of which are starch, such as flynn, sugar and galactan. In oriental medicine, taro has been used to cool down the body and reduce inflammation, while in the private world, blood-blown and crushed taro and flour mixtures are applied to lymphitis, dermatitis, hemorrhoids and bruises. In addition, it has a diuretic effect, lowering the heat in the stomach and facilitates gastrointestinal movement. However, there are few studies on the ingredients of taro as a cosmetic and on the whitening activity.

An object of the present invention is to provide a cosmetic composition for whitening comprising a extract of cortex of Colocasia antiquorum to inhibit melanin production by inhibiting tyrosinase activity.

In order to achieve the above object, the present invention provides a cosmetic composition for whitening as an active ingredient taro extract.

The extract means an extract soluble in water, lower alcohols having 1 to 4 carbon atoms, or a mixed solvent thereof, preferably a mixed solvent of water and methanol, more preferably 50 to 100% methanol.

The extract refers to extraction methods such as cold sediment extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction or low to medium pressure extraction, preferably an extract obtained by ultrasonic extraction or low to medium pressure extraction.

Hereinafter, the present invention will be described in detail.

The tarope extract of the present invention is dried after washing the taropi with water, and about 1 to 30 times its weight, preferably about 10 to 20 times the volume of water, lower alcohol having 1 to 4 carbon atoms or about 1: Mixed solvent having a mixing ratio of 0.1 to 1:10, preferably 1: 0.2 to 1: 3, preferably about 0.5 to 48 at an extraction temperature of 10 to 100 캜, preferably 15 to 30 캜 with 85% methanol Extraction method such as cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction for 1 to 36 hours, preferably after repeated extraction several times by ultrasonic extraction method, the extract is centrifuged and filtered and decompressed at 45 ° C. Concentration can yield the toranpy extract of the present invention.

In addition, the method of obtaining a toranfi extract of the present invention uses a low to medium pressure extraction method,

For example, the taro medium low pressure extract of the present invention is washed with water to dry the taro after drying, about 10 to 30 times the weight of water, preferably about 10 to 20 times the volume of water, lower alcohol having 1 to 4 carbon atoms Or an extraction temperature of 10 to 120 ° C., preferably 80 to 100 ° C., with a mixed solvent having a mixing ratio of about 1: 0.1 to 1:10, preferably 1: 0.2 to 1: 3, preferably 85% methanol After repeated extraction several times at a pressure of 1 bar to 10 bar, preferably 2.8 bar for about 0.5 to 48 hours, preferably 1 to 24 hours in the extract is concentrated under reduced pressure at 45 ℃ after centrifugation and filtration Toranpi low pressure extract of can be obtained.

In addition, conventional fractionation processes can also be performed (Harborne, JB, Phytochemical methods: A guide to modern techniques of plant analysis, 3rd Ed. , Pp 6-7, 1998).

The composition of the present invention comprises the extract in an amount of 0.01 to 10% by weight, preferably 0.1 to 8% by weight, most preferably 0.5 to 5% by weight, based on the total weight of the composition.

As a result of measuring the inhibitory activity against tyrosinase, a major enzyme involved in melanin biosynthesis of the toranphi extract obtained by the above extraction method, the toranpy extract inhibits tyrosinase excellently and strongly inhibits melanin production. By doing so, it could be confirmed that it can be used as a functional cosmetic composition for whitening.

The composition containing the toranpy extract of the present invention can be used in various ways, such as cosmetics and face wash for skin whitening effect. Examples of products to which the present composition can be added include cosmetics such as various creams, lotions, skins, and the like, cleansing agents, face washes, soaps, treatments, and essences.

Cosmetics of the present invention comprises a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids and seaweed extract.

The water-soluble vitamins may be any compound that can be incorporated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H, and the like. Their salts (thiamine hydrochloride, sodium ascorbate salt, etc.) and derivatives (ascorbic acid-2-sodium phosphate salt, ascorbic acid-2-magnesium phosphate salt, etc.) may also be used in the water-soluble vitamins that can be used in the present invention. Included. The water-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification from microorganism culture, enzyme or chemical synthesis.

The oil-soluble vitamin may be any compound that can be incorporated into cosmetics, but preferably vitamin A, carotene, vitamin D2, vitamin D3, vitamin E (d1-alpha tocopherol, d-alpha tocopherol, d-alpha tocopherol), and the like. And derivatives thereof (ascorbic palmitate, ascorbic stearate, ascorbic acid dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherolvitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl) Ethers, etc.) are also included in the oil-soluble vitamins used in the present invention. Oil-soluble vitamins can be obtained by conventional methods such as microbial transformation, purification of microorganism culture, enzyme or chemical synthesis.

The polymer peptide may be any compound as long as it can be incorporated into cosmetics. Preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like can be given. Polymeric peptides can be purified and obtained by conventional methods such as purification from microbial cultures, enzymatic methods or chemical synthesis methods, or can be purified and used from natural products such as dermis and pig silk such as pigs and cattle.

The polymer polysaccharide may be any compound as long as it can be blended into cosmetics. Preferably, hydroxyethyl cellulose, xanthan gum, sodium hyaluronate, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be mentioned. For example, chondroitin sulfate or its salt, etc. can be normally purified from a mammal or fish.

The sphingolipid may be any compound as long as it can be blended into cosmetics. Preferably, ceramide, phytosphingosine, sphingosaccharide lipid, etc. may be mentioned. Sphingo lipids can usually be purified from mammals, fish, shellfish, yeasts or plants by conventional methods or obtained by chemical synthesis.

The seaweed extract may be any compound as long as it can be blended into cosmetics. Preferably, the seaweed extract may include brown algae extract, red algae extract, green algae extract, and the like. Also, calginine, arginic acid, sodium arginate, Potassium arginate and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purification from seaweed by conventional methods.

In addition to the said essential component, you may mix | blend the cosmetics of this invention with the other components normally mix | blended with cosmetics as needed.

Other components that may be added include fats and oils, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, flavorings, Blood circulation accelerators, cooling agents, restriction agents, purified water and the like.

Examples of the fat or oil component include ester fats, hydrocarbon fats, silicone fats, fluorine fats, animal fats, and vegetable fats and oils.

As ester fats and oils, glyceryl tri2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl mystinate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, and stearic acid Butyl, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl acid isocetyl, isostyl acid isostearyl, isostaryl palmitate, octylate acid octyldodecyl, isostearic acid isetyl, diethyl sebacate, adipine Acid isopropyl, isoalkyl neopentane, tri (capryl, capric acid) glyceryl, trimethyl ethyl trimethylolpropane, trimethyl stearate trimethylol propane, tetra 2-ethyl hexanoate penta erythritol Cetyl caprylate, lauric acid decyl, hexyl laurate, decyl myristin, myristin acid myristyl, myritic acid cetyl, stearyl stearate, decyl oleate, rininooleic acid Teyl, isostearyl laurate, isotridecyl myristin, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyl dodecyl oleate, octyl dodecyl linoleate, isopropyl isopropyl acid, 2-ethylhexanoate cetostearyl, 2-ethylhexanoate stearyl, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicapric acid, propylene glycol di (capryl, capric acid), Dicaprylic acid propylene glycol, dicapric acid neopentyl glycol, dioctanoate neopentyl glycol, tricaprylate glyceryl, triundecyl glyceryl, triisopalmitinate glyceryl, triisostearate glyceryl, octylate octadodode Sil, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isstearyl isostearate, Sothete octylate, polyglycerol oleate, polyglycerine isostearate, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myritic lactate, octyl lactate, octyl lactate Ethyl, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malic acid, 2-ethylhexyl hydroxystearate, di2-ethylhexyl succinate, diisobutyl adipicate, diisopropyl sebacinate, Dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, physteryl isostearate, phytic oleate, 12-Steloylhydroxystearate isocetyl, 12-stealoylhydroxystearate stearyl, 12-steal One hydroxy stearic acid and the like esters such as cetearyl source.

Hydrocarbon-based fats and oils, such as squalene, a liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, a liquid isoparaffin, polybutene, microcrystal wax, and a vaseline, etc. are mentioned as a hydrocarbon-type fats and oils.

Examples of the silicone-based oils and fats include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane, methylcetyloxysiloxane copolymer, dimethylsiloxane and methylsteoxysiloxane copolymer, and alkyl. Modified silicone oil, amino modified silicone oil and the like.

Perfluoro polyether etc. are mentioned as fluorine-based fats and oils.

Animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil. , Cottonseed oil, Palm oil, Cucumber nut oil, Wheat germ oil, Rice germ oil, Shea butter, Walnut colostrum oil, Marker demia nut oil, Meadow home oil, Egg yolk oil, Uji, Horse oil, Mink oil, Orange rape oil, Jojoba oil And animal or plant fats and oils such as candeler wax, carnava wax, liquid lanolin and hardened castor oil.

Examples of the moisturizing agent include a water-soluble low molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

As the water-soluble low molecular humectant, serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), Polypropylene glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactic acid salt, etc. are mentioned.

Examples of the fat-soluble low molecular humectants include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyasparaginate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water soluble chitin, chitosan, and dextrin. Can be.

Examples of the fat-soluble polymers include polyvinylpyrrolidone-eicosene copolymers, polyvinylpyrrolidone-hexadecene copolymers, nitrocellulose, dextrin fatty acid esters, polymer silicones, and the like.

Examples of the emollient include long-chain acyl glutamic acid cholesteryl esters, hydroxy stearic acid cholesterol, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl esters, and the like.

As surfactant, nonionic surfactant, anionic surfactant, cationic surfactant, amphoteric surfactant, etc. are mentioned.

As the nonionic surfactant, self-emulsifying glycerin monostearate, propylene glycol fatty acid ester, glycerin fatty acid ester, polyglycerol fatty acid ester, sorbitan fatty acid ester, POE (polyoxyethylene) sorbitan fatty acid ester, POE sorbitan fatty acid ester, POE Glycerin fatty acid ester, POE alkyl ether, POE fatty acid ester, POE hardened castor oil, POE castor oil, POE / POP (polyoxyethylene / polyoxypropylene) copolymer, POE / POP alkyl ether, polyether modified silicone, lauric acid Alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, etc. are mentioned.

Anionic surfactants include fatty acid soaps, alpha-acyl sulfonates, alkyl sulfonates, alkyl allyl sulfonates, alkyl naphthalene sulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkylphosphates, and alkylamides. Phosphates, alkyloylalkyltaurine salts, N-acylamino acid salts, POE alkyl ether carboxylate salts, alkyl sulfosuccinate salts, sodium alkyl sulfo acetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, and the like. have.

As cationic surfactant, alkyl trimethylammonium chloride, stearyl trimethyl ammonium chloride, stearyl trimethyl ammonium chloride, cetostearyl trimethyl ammonium chloride, distearyl dimethyl ammonium chloride, stearyl dimethyl benzyl ammonium bromide, behenyl trimethyl ammonium chloride, chloride Benzalkonium, diethylaminoethyl stearate, dimethylaminopropyl stearate, lanolin derivatives, quaternary ammonium salts, and the like.

Examples of amphoteric surfactants include the carboxybetaine type, the amide betain type, the sulfobetain type, the hydroxysulfobetain type, the amide sulfobetain type, the phosphobetaine type, the aminocarboxylate type, the imidazoline derivative type, and the amideamine type. An amphoteric surfactant etc. are mentioned.

Organic and inorganic pigments include silicic acid, silicic anhydride, magnesium silicate, talc, sericite, mica, kaolin, bengala, clay, bentonite, titanium film mica, bismuth oxychloride, zirconium oxide, magnesium oxide, zinc oxide, titanium oxide, aluminum oxide Inorganic pigments such as calcium sulfate, barium sulfate, magnesium sulfate, calcium carbonate, magnesium carbonate, iron oxide, ultramarine blue, chromium oxide, chromium hydroxide, calamine and composites thereof; Polyamide, polyester, polypropylene, polystyrene, polyurethane, vinyl resin, urea resin, phenol resin, fluorine resin, silicon resin, acrylic resin, melamine resin, epoxy resin, polycarbonate resin, divinylbenzene, styrene copolymer, Organic pigments such as silk powder, cellulose, CI pigment yellow, CI pigment orange, and composite pigments of these inorganic pigments and organic pigments;

As organic powder, Metal soaps, such as a calcium stearate; Alkyl phosphate metal salts such as sodium cetylinate, zinc lauryl acid and calcium laurate; Acylamino acid polyvalent metal salts such as N-lauroyl-beta-alanine calcium, N-lauroyl-beta-alanine zinc, and N-lauroylglycine calcium; Amide sulfonic acid polyvalent metal salts, such as N-lauroyl-taurine calcium and N-palmitoyl-taurine calcium; N-epsilon-lauroyl-L-lysine, N-epsilon-palmitolyzine, N-alpha-paratoylol nitin, N-alpha-lauroyl arginine, N-alpha-cured fatty acid acyl arginine -Acyl basic amino acid; N-acyl polypeptides, such as N-lauroyl glycyl glycine; Alpha-amino fatty acids such as alpha-aminocaprylic acid and alpha-aminolauric acid; Polyethylene, polypropylene, nylon, polymethyl methacrylate, polystyrene, divinylbenzene-styrene copolymer, ethylene tetrafluoride and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoic acid, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomentyl salicylic acid and benzyl cinnamic acid. , Paramethoxy cinnamic acid-2-ethoxyethyl, paramethoxy cinnamic acid octyl, diparamethoxy cinnamic acid mono-2-ethyl hexane glyceryl, paramethoxy cinnamic acid isopropyl, diisopropyl diisopropyl cinnamic acid ester mixture, wuro Canonic acid, ethyl urokanoate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium sulfonate, dihydroxybenzophenone , Tetrahydroxybenzophenone, 4- tert -butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino- p- (carbo-2'-ethylhexyl-1'-oxy) -1 , 3,5-triazine, 2- (2-hi And the like can be mentioned hydroxy-5-methylphenyl) benzotriazole.

As fungicides, hinokithiol, trichloric acid, trichlorohydroxydiphenyl ether, chlorhexidine gluconate, phenoxyethanol, resorcin, isopropylmethylphenol, azulene, salicylic acid, ginxitlionone, benzalkonium chloride, photosensitive Sodium No. 301, sodium mononitroguicol, undecylenic acid, and the like.

Examples of the antioxidant include butylhydroxyanisole, propyl gallic acid, and erythorbic acid.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, fmaric acid, sodium pmarate, succinic acid, sodium succinate, sodium hydroxide, sodium dihydrogen phosphate, and the like.

Examples of the alcohol include higher alcohols such as cetyl alcohol.

Moreover, the compounding component which may be added other than this is not limited to this, Moreover, Although all said components can be mix | blended within the range which does not impair the objective and effect of this invention, Preferably it is 0.01-5% weight percentage with respect to a total weight, More preferably 0.01-3% by weight.

The cosmetic of the present invention may take the form of a solution, an emulsion, a viscous mixture, or the like.

Ingredients included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the extract as an active ingredient, for example, conventional auxiliary agents such as stabilizers, solubilizers, vitamins, pigments and flavorings. And carriers.

The functional cosmetic composition for whitening of the present invention may be prepared in any formulation commonly prepared in the art, and includes, for example, emulsion, cream, lotion, pack, foundation, lotion, essence, hair cosmetic, and the like.

Specifically, the cosmetic composition of the present invention skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisturizing lotion, nutrition lotion, massage cream, nutrition cream, moisturizing cream, hand cream, foundation, essence, nutrition essence, Formulations of packs, soaps, cleansing foams, cleansing lotions, cleansing creams, body lotions and body cleansers.

When the formulation of the present invention is a paste, cream or gel, animal carriers, vegetable fibers, waxes, paraffins, starches, tracantes, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide, etc. may be used as carrier components. Can be.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane Propellant such as butane or dimethyl ether.

When the formulation of the invention is a solution or emulsion, a solvent, solvating or emulsifying agent is used as the carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 Fatty acid esters of, 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the dosage form of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

When the formulation of the present invention is a surfactant-containing cleansing, the carrier component is an aliphatic alcohol sulfate, an aliphatic alcohol ether sulfate, a sulfosuccinic acid monoester, an isethionate, an imidazolinium derivative, a methyltaurate, a sarcosinate, a fatty acid amide. Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, linolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

Hereinafter, the present invention will be described in detail by the following Examples, Reference Examples and Experimental Examples.

However, the following Examples, Reference Examples and Experimental Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples, Reference Examples and Experimental Examples.

Example 1.Toranpi extract preparation

1-1. Ultrasonic Extraction Manufacturing

After extracting the taro, dry it with a grinding machine and grind it into fine powder, 100 g of the fine powder sample was extracted with 1.5 L of 85% methanol at 15-27 ° C. three times for 1 hour, and then vacuum filtered. The filtrate was collected by repeating the process of recovering the filtrate twice. Concentrated under reduced pressure at 45 ℃ and lyophilized to give 5.9 g of the taroic ultrasonic extract.

1-2. Manufacture of low and medium pressure extraction method

After extracting dry taro, dry it with a grinding machine to make a fine powder, a medium-low pressure reactor (2.8 bar pressure), which is a reactor manufactured by Miwon Co., Ltd., 60-120 ° C. with 100 ml of 85% methanol in 100 ml. After stirring and extraction for 3 hours, the filtrate was collected by vacuum filtration to collect the filtrate. Concentrated under reduced pressure at 45 ℃, and lyophilized to give 0.73 g of tarophytic low pressure extract.

Reference Example 1. Experiment Preparation

1-1. Tyrosinase Extract

Tyrosinase was extracted directly from melan-A cells. Extraction method was performed by adding 200 μl of tyrosinase buffer (80 mM PO ₄ buffer + 1% Triton-X 100 + 100 mg / ml PMSF) into a 100π tissue culture dish filled with melan-A cells, and separating the cells. After sonicating in an ice basket, the supernatant was obtained by centrifugation at 12500 rpm for 15 minutes. The obtained supernatant is obtained by protein quantification to obtain a total weight value and then put into 150 μg of protein in each well.

1-2. Experimental Samples and Reagents

 L-dopa (product number: D9682) and TPA (phobol 12- Myristate 13- Acetate; product number: P3135) were also purchased from Sigma, and Kojisan (product number: K3125) was manufactured by Aldrich. It was purchased and used.

 In addition, penicillin-streptomycin (product number: 15140-122), fetal bovine serum (FBS, product number: 26140-079), RPMI1640 (product number: 31800-014) were purchased from Gibco. Used.

1-3. Instrument analysis

Tyrosinase activity analysis was performed using a spectrophotometer (precision microplate reader; model name: E09090, Molecular Devices).

Experimental Example 1. Confirmation of inhibitory effect on melanin production-related enzyme tyrosinase

Tyrosinase inhibitory activity involved in melanin biosynthesis is described by Aroca et al., J. Biol. Chem. 268 (34) , p25650-25655, 1993 and Tomita et al. (Tomita et al. J. Antibiot. 43 (12) , pp1601-1604, 1990).

The present invention examined the inhibitory effect on tyrosinase, an enzyme associated with melanogenesis, in order to confirm the whitening activity of the tarophyll extract of Example 1. A test sample (100 ppm, 250 ppm) dissolved in 120 µl of 8 mM L-dopa (dissolved in 67 mM phosphate buffer pH 6.8) and 40 µl of methanol was added to a 96-well microplate and 150 µl of melan-A cell extraction tyrosinase was added. It was. After incubation at 37 ° C. for 20 minutes, the absorbance at 492 nm was measured using a precision microplate reader (Molecular Devices E09090). Tyrosinase inhibitory effect was used in reference to Matsuda et al. (Matsuda, et al., Biol. Pharm. Bull. , 19 (1) , pp153-153, 1996), and the following equation 1 was used. Based on the control group.

The tyrosinase inhibitory activity of the toranphi extract is shown in Table 1 below. Toranpi extract significantly inhibited tyrosinase at 250ppm concentration, it was confirmed that the extract of the present invention can be usefully used as a cosmetic for whitening.

sample Concentration (ppm) Inhibition Rate (%) Taro 100 12.05 ± 8.0 250 17.08 ± 3.3 Kojisan 100 41.67 ± 2.0 250 54.14 ± 10.7  * Each figure is the average of three experiments

Experimental Example 2. Melan-A Cell Experiment

For a method of verifying the effect of inhibiting melanin production using melan-A cells, see Bennet et al. (Bennette et al., Int. J. Cancer. 39 , pp414-418, 1987).

2-1. Cell culture

Melan-A cell line in Fetal Bovine Serum (No. 26140-079) and 200nm Phorbol 12-Myristate 13-Acetate in RPMI medium (31800-14) Cultured under P3135) conditions. 10 ml of the culture solution is placed in a 100 [pi] tissue culture dish and about 5 x 10 ⁵ cells are seeded. After 3-4 days at 37 ° C. and 5% CO ₂ , the cells were fused and grown at 10 ⁵ cells / well in 24 well plates for 24 hours.

990 µl of the culture medium was changed daily, and 10 µl of the sample was treated for 3 days (solvent: 50% propylene glycol, 30% ethanol, 20% water), and then cultured for 1 day.

2-2. Melanin content measurement

After removing the medium from the cells cultured by the method of Example 2-1 to determine the melanin content, the cells were washed with PBS. Melanin was dissolved using 1 ml of 1N sodium hydroxide, and the absorbance was measured at 400 to 405 nm.

2-3. Cell viability measurement

In order to measure cell viability, the medium of Example 2-1 was removed, washed with PBS, and cultured at room temperature for 5 minutes by adding 200 μl of crystal violet (CV 0.1%, 10% ethanol, remaining PBS) reagent. Then washed twice with water. After adding 1 ml of ethanol and shaking for 10 minutes at room temperature, the absorbance was measured at 590 nm.

As a result of the experiment, as shown in Table 2 and Figure 1 it was confirmed that the excellent suppression of melanin production at the concentration of 500ppm, and also can be usefully used as a cosmetic for whitening significantly high cell survival rate.

Concentration (ppm) Melanin (%)  Cell survival rate (%) Taro extract 100 91.79 35.00 250 60.90 106.65 500 31.34 98.93

The toranpy extract of the present invention can be prepared in the following formulations, the following formulations are merely illustrative of the present invention, whereby the content of the present invention is not limited.

Formulation Example 1. Bath solution

A bath liquid may be prepared by mixing 21 parts by weight of DL-sodium lactate, 8 parts by weight of sodium pyruvate, 5 parts by weight of tarophytic extract, 40 parts by weight of ethanol, 26 parts by weight of purified water, and an appropriate amount of a pigment and a perfume. When used, this product is diluted 100-10,000 times in bath water, making it suitable for use as a skin beauty and skin whitening agent. In this case, a cleansing liquid, astringent liquid and a moisturizing liquid can be used instead of the bath water.

Formulation Example 2 Skin Lotion

3 parts by weight of glycerin, 2 parts by weight of butylene glycol, 2 parts by weight of propylene glycol, and preservatives were added and dissolved in purified water, followed by stirring to dissolve 1 part by weight of polyoxyethylene (60) hardened castor oil and dissolved therein. Was added and stirred to add 80.9 parts by weight of purified water. Finally, 1 part by weight of the tarophyl extract powder, 10 parts by weight of ethanol, 0.1 parts by weight of triethanol amine, and a pigment were added thereto, followed by sufficiently stirring to obtain the title product. It is used as a high quality sunscreen, skin care and skin whitening agent.

Formulation Example 3 Milk Lotion (Nutrition Lotion)

 Cytosterol 1.7 parts by weight, polyglyceryl 2-oleate 1.5 parts by weight, ceramide 0.7 parts by weight, Ceteareth-4 1.2 parts by weight, cholesterol is homogenized at a constant temperature, 1 part by weight of tarophyl extract powder and dicetyl phosphate 0.4 parts by weight, concentrated glycerin 5.0, 10 parts by weight of sunflower oil, 0.2 parts by weight of carboxyvinyl polymer, 0.3 parts by weight of xanthan gum, and preservatives were dissolved by heating in a conventional manner, and 76.5 parts by weight of purified water was added to this solution, followed by emulsification in a homogenizer. Then, an appropriate amount of fragrance was added and stirred and mixed again to obtain the title product. It is used as a high quality sunscreen, skin care and skin whitening agent.

Formulation Example 4 Nutrition Cream

 4 parts by weight of cytosterol, 3 parts by weight of polyglyceryl 2-oleate, 0.7 parts by weight of ceramide, 2 parts by weight of Ceteareth-4, 3 parts by weight of cholesterol, homogenized at a constant temperature, 3 parts by weight of toranpy extract powder and dicetylphosphate 0.4 parts by weight, concentrated glycerin 5.0, 22 parts by weight of sunflower oil, 0.5 parts by weight of carboxyvinyl polymer, and preservatives were dissolved by heating in the usual manner, 56.4 parts by weight of purified water was added to this solution, and then emulsified in a homogenizer. It was added and stirred and mixed again to obtain the title product. It is used as a high quality sunscreen, skin care and skin whitening agent.

Formulation Example 5 Massage Cream

 0.2 parts by weight of carboxy vinyl polymer, 5 parts by weight of glycerine, 3 parts by weight of butylene glycol, 3 parts by weight of propylene glycol, 22.3 parts by weight of purified water, and a small amount of preservatives were mixed and stirred at 80-85 ° C. 10 parts by weight of beeswax, 1.5 parts by weight of polysorbate 60, 0.8 parts by weight of sorbitasquioleate, 40 parts by weight of liquid paraffin, 5.0 parts by weight of squalane, 4 parts by weight of caprylic / capric triglycerides, triethanol 0.2 parts by weight of amine is heated to 80-85 ° C., and then emulsified. After emulsification, the mixture was cooled to 50 ° C. while stirring with a stirrer, and then the pigment was added and cooled to 45 ° C., then the fragrance was added, and when cooled to 35 ° C., 5 parts by weight of tarophyl extract was added and stirred and mixed again. Got it. It is used as a high quality sunscreen, skin care and skin whitening agent.

Formulation Example 6 Nutrition Essence

Cytosterol 1.7 parts by weight, polyglyceryl 2-oleate 1.5 parts by weight, ceramide 0.7 parts by weight, Ceteareth-4 1.2 parts by weight, cholesterol 1.5 parts by weight homogenized at a constant temperature, and 5 parts by weight of tarophyl extract powder and di 0.4 parts by weight of cetylphosphate, 5.0 parts by weight of concentrated glycerin, 15 parts by weight of sunflower oil, 0.2 parts by weight of carboxyvinyl polymer, 0.2 parts by weight of xanthan gum, and a preservative by dissolving it in a conventional manner and adding 67.6 parts by weight of purified water to this solution. After emulsifying at, an appropriate amount of perfume was added and stirred and mixed again to obtain the title product. It is used as a high quality sunscreen, skin care and skin whitening agent.

As described above, the toranphi extract of the present invention has an excellent inhibitory activity against tyrosinase involved in melanin biosynthesis and inhibits melanin production and thus can be usefully used as a cosmetic composition for whitening.

Claims (3)

A whitening cosmetic composition containing the extract of taro as an active ingredient. The cosmetic composition according to claim 1, wherein the extract is extracted by an extraction method of cold sediment extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, and low to medium pressure extraction. The composition of claim 1, wherein the composition is a skin lotion, a skin softener, a skin toner, an astringent, a lotion, a milk lotion, a moisturizer, a nutrition lotion, a massage cream, a nutrition cream, a moisturizer cream, a hand cream, a foundation, an essence, a nutrition essence, a pack Cosmetic composition, characterized in that it has at least one formulation selected from the group consisting of soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

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