KR20070025066A - Herbal composition for treatment and prevention of diabetes and diabetic complications - Google Patents

Abstract

A herbal composition is provided to effectively inhibit vascular disorder, which is a main symptom of diabetes, thereby effectively preventing and treating diabetic complications such as hyperlipidemia and arteriosclerosis. The herbal composition for preventing and treating diabetes and diabetic complications comprises 7-13 parts by weight of Glycyrrhiza uralensis, 7-13 parts by weight of Nelumbinis Semen, 7-13 parts by weight of Liliope koreana NAKAI, 7-13 parts by weight of Psyllium, 7-13 parts by weight of Scutellaria baicalensis Georgi, 7-13 parts by weight of poria cocos Wolf, 9-17 parts by weight of Astragalus membranaceus, 7-13 parts by weight of Eucommia ulmoides, 7-13 parts by weight of cow gall, 7-13 parts by weight of silkworm excreta, 7-13 parts by weight of Artemisia capillaris Thunberg, 7-13 parts by weight of Rhynchosia Nulubilis, 7-13 parts by weight of Bombycis corpus, 7-13 parts by weight of dried roots of Trichosantes kirilowii maxim, 2-4 parts by weight of Pleuropterus multflorus, 2-4 parts by weight of calcite, 2-4 parts by weight of ginseng and 2-4 parts by weight of egg shell regarding 20 parts by weight of Nelumbinis Semen.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a herbal composition for diabetes and diabetic complications,

FIG. 1 is a graph showing changes in plasma glucose levels in the control group, the experimental group and the comparative group according to the days of administration of streptozotocin.

FIG. 2 is a graph showing the mean arterial pressure changes of the control group, the experimental group and the comparative group according to the days of administration of streptozotocin.

FIG. 3 is a graph showing changes in aortic relaxation rate of the control group, the experimental group, and the comparative group according to the concentration of the extract of the present invention after KCl shrinkage.

FIG. 4 is a graph showing changes in aortic contraction ratio of the control group, the experimental group, and the comparative group according to the norepinephrine administration concentration.

FIG. 5 is a graph showing changes in aortic relaxation rate of the control group, the experimental group, and the comparative group according to the acetylcholine administration concentration.

The present invention relates to a herbal composition for the treatment and prevention of diabetes and its complications, and more particularly, to a herbal composition for treating and preventing diabetic complications such as diabetes, arteriosclerosis and hyperlipemia by effectively inhibiting the vascular disorder phenomenon belonging to the main symptom of diabetes ≪ / RTI >

Diabetes mellitus is the most common non-communicable disease occurring in modern people. When the insulin produced in the pancreas is deficient or abnormal, it is not properly stored in the liver, muscle, or fat cells in the liver, The fasting blood glucose level is 60-120 mg / dl, which is much higher than the normal blood glucose level of the normal person who does not exceed 140 mg / dl after 2 hours of eating. Such excessive amount of blood sugar causes damage to the gonadal sphere, Or excreted, or become cholesterol or triglycerides in the blood. In addition, it can cause complications such as hypertension, nephritis, stroke, heart disease, arteriosclerosis, and hyperlipemia. Therefore, it is very important to prevent further development and prevent complications from occurring.

In particular, in patients with diabetes, arteriosclerotic lesions of the coronary arteries, cerebral vessels and peripheral blood vessels occur more frequently than non-diabetic patients, and such vascular complications are known to be involved in about 80% of adult diabetic deaths. In addition, atherosclerotic disease, hypertension, and lipid metabolism abnormality in diabetic patients often show different symptoms and progression from those occurring in general adults, so attention should be paid to prevention and treatment.

The incidence of hypertension in diabetic patients is about 1.5 to 2 times higher than in non-diabetic adults. In the case of insulin-dependent diabetes mellitus, the blood pressure is usually normal at the onset of diabetes, and the frequency of hypertension is almost similar to that of the general public. This state is maintained for 5 to 10 years and diabetic nephropathy occurs, . On the other hand, patients with non-insulin-dependent diabetes mellitus often have hypertension when they are diagnosed with diabetes and appear before the renal dysfunction, and the incidence of hypertension increases when diabetic nephropathy is present. Although diabetic patients are accompanied by a slight increase in blood pressure, they are affected by various vascular complications, microvascular complications, and decreased renal function.

In the Oriental medicine, about 700 BC, the embarrassment of the Emperor of the Inner Circle, indicating the common consumable disease is one of the disease began to deal with diabetes. The treatment method, which administers acupuncture treatment combined with acupuncture treatment for 2 to 3 months, has been used as an old treatment method while intensively administering medicines such as chrysanthemum, rhubarb, and creation. Another folk remedy has been to use hemp, which is also called chrysanthemum and scab. Chunghwa has long been known as a covenant of diabetes, which is good for eliminating thirst due to diabetes, and it is known to protect the stomach of diabetic patients.

Recently, there have been various studies on the treatment of diabetes using medicinal herbs. Korean Patent Laid-Open Publication No. 2005-70704 discloses that Moutan Radicis Cortex extract used in oriental medicine increases the action of insulin in 3T3-L1 adipocytes to increase glucose uptake and significantly suppresses alpha-glucoamylase activity , And that the composition has an excellent blood-sugar-enhancing effect, and is disclosed as a composition for the prevention and treatment of diabetes mellitus. Korean Patent Laid-Open Publication No. 2005-70704 discloses a composition for preventing and treating diabetes mellitus containing an extract of forsythiae fructus which exhibits excellent activity to enhance the activity of AMPK (5'AMP-activated protein kinase) as an active ingredient . In addition to these, methods for the prevention and treatment of diabetes using various herbal medicines or their extracts have been studied.

Korean Patent Laid-Open Nos. 2003-59719, 2003-59951, and 2003-59952 disclose a combination of herb extracts of pure ginseng, pseudo-excrements and marine pests, which are used for the treatment of diabetes-related diseases, A composition for prevention and treatment of diabetes mellitus is disclosed, which is intended to be used as a preparation for pharmaceutical use.

It is expected that not only a known herbal medicine but also various herbal medicines properly mixed with each other will be able to produce a composition showing more excellent effects in the prevention and treatment of diabetes.

The present inventors have conducted extensive research on various herbal medicines used as herbal ingredients. As a result, it has been found that when the herbal composition of the present invention including 19 kinds of herbal ingredients including soft meat is used, the prevention and treatment of diabetes and its complications And thus the present invention has been completed.

It is an object of the present invention to provide a herbal composition and its extract which can prevent and treat diabetes and diabetic complications.

In order to achieve the above object, the herbal composition for prevention and treatment of diabetes and its complications according to the present invention comprises 7 to 13 parts by weight of licorice, 7 to 13 parts by weight of ginger, 7 to 13 parts by weight of gumbo 7 to 13 parts by weight of car electron, 7 to 13 parts by weight of gold, 7 to 13 parts by weight of Baekbokryeong, 9 to 17 parts by weight of sulfur, 7 to 13 parts by weight of beeswax, 7 to 13 parts by weight of beeswax, 7 to 13 parts by weight of starch, 7 to 13 parts by weight of epidermis, 7 to 13 parts by weight of white moth, 7 to 13 parts by weight of silkworm, 2 to 4 parts by weight of sewage, 2 to 4 parts by weight of ginseng, And 2 to 4 parts by weight of lanthanum.

Hereinafter, the present invention will be described in more detail.

The herbal composition for preventing and treating diabetes mellitus and its complications according to the present invention is characterized in that it comprises at least one compound selected from the group consisting of lignocellulosum, licorice, gigantic skeleton, maemundong, There are three kinds of gold (黄 芩), Baekbokryeong (黄 茯 朞), 黄 朞 (杜 冲), Udam (牛 膽), 蠶 糞 (蔯 糞), 仁 蔯 (茵 蔯) (白 蠶 蠶), chrysanthemum powder (....), 하... (首..........), Ginseng, ginseng and egg shell.

Nelumbinis semen is the seed of the seeds, which stabilizes the body and mind, relaxes the five, calms and tonic. It is a medicinal herb that has effects on the mental nervous system such as nervous breakdown, insomnia, chest leap, forgetfulness, anxiety neurosis, and depression. In addition, it is good for the recovery of fatigue, relieves the thirst, reduces blood pressure and helps to sleep. Folk remedies are used together with medicinal herbs such as white moths and twins, and they are sometimes taken as 'Paekcheon'.

Licorice (甘草; Glycyrrhiza uralensis) acts to neutralize all drug components. <Dongbu Bohak>> 'Licorice is 5 to 6 parts of the heat (热气) (邪气) to reign over the eyes of the nose and ears and feces to normal menstruation to communicate all the blood vessels, muscles and bones and strengthen the nutritional status Not only does it detoxify the toxicity of all medicines, but also makes it possible to harmonize the 72 kinds of stone medicine and 1200 kinds of herbal medicine to make them appear better. " Licorice is antagonistic to various polar drugs and toxic drugs to treat drug poisoning caused by poison or poison, neutralizing and detoxifying poison caused by bacteria. It is also known to have an anti-allergic effect, which is effective against gastric and duodenal ulcers. It also works as a corticosteroid to relieve stress and relieve pain and nerve tension caused by muscle tension. have.

Licii Radicis Cortex is the roots of Gujuga tree. It is sweet and cold, and mainly acts on the lungs, liver and kidneys. Cheongryeongbyeol (淸 熱 凉血) to cool the heat to cool the blood and cold lung heat (淸 肺热) to cool the lungs. It is effective for symptoms such as seawater caused by waste heat, asthma, hematochezia, nosebleeds, hemorrhage of urine, osteoporosis (osteoporosis fever, exhaustion diseases such as tuberculosis), and lowering of blood sugar and also effects on cardiovascular system, It is known.

It is known that McMundong (Liliope koreana NAKAI) is effective in nourishing the lungs, strengthening the stomach, dissolving the heat in the body, and making the body fluid and body fluids necessary for the body such as lymph fluid and cell fluid. According to pharmacological experiments, there is also arteriosclerosis, diuretic action, sputum sagging, coughing, and nutrition. See the woman's voice, relax the lungs, control the deep-seated (deep-seated disease) and urinate well. Lung and stomach, especially in view of the effect of gymnastics, especially excellent for the weakness of the body is commonly used to weaken the strength of the power comes out. It also helps to get rid of constipation. It is known that the throat and the mouth are dried, and the thirst that occurs is dried.

Psyllium contains not only a large amount of mucus but also a rich protein and fatty acid, so it has an excellent effect of lowering the intraocular pressure and promoting diuretic action. In addition, dietary fiber is abundant and is effective in constipation. It is said to be especially good for men who have difficulty in urinating and whose stamens are weak, those who eat good heat and diarrhea in summer, and those who have dark eyes and have no sex.

Gold (黄 芩; Scutellaria baicalensis Geoge) is also called 'spiny grass', which removes waste heat and removes wet heat from the large intestine. In Dong-bo-bo-gak, 'the nature is garage [寒], the taste is [bitter], and there is no poison. It treats heat poisoning, osteoporosis, cold, and fever, stops the thirst of heat, and heals jaundice, dysentery, diarrhea, bloating, and stomach fever. It makes the small intestine well, and it heals the eugen, the lapel, the glaze and the fever fever [天 行 熱 病]. It is possible to swallow fire in the lungs because it is dry and ineffective, and it makes the wall curl, and it makes the circulation well. It enters the conception penis, and the roots are thin and hard, and not hollow, heals the disease of hemp, and buys the fire of the captain. I write in the medicine to sink in water. If you stir up the liquor, the energy of the liquor goes up. If you fry it, it goes down. I usually use raw things [introductory]. "

Baek Bok-ryeong (白 茯 笭) is also called 'Bonsolsol bongryeong'. The taste is sweet and bored, the quality is flat, and there is a function to complement, and diuretic action, but not strong. It has long been used as a tonic. It works on menopause (肺 经), necropsy (脾 经), heart (心 经), nerve (肾 经) and bladder (膀 经) to see the spleen, Pharmacological experiments have shown that diuretic action, lowering of blood sugar level, sedation, etc. It is also known that the immune revival effect. The spleen is hung (虛) to swell the body and used in the bottle. In addition, he also prescribes dewar, diarrhea, amnesia, and sleep disorders. It is known to have excellent effects on diseases such as stomach, liver, stomach, and kidney, and is used as a medicament for the treatment of a person who is weak after suffering from a disease and a patient with chronic gastroenteritis. It is also known to be effective against sexually transmitted diseases as well as sexually transmitted diseases and women's diseases.

Hwanggi (黃 朞) is sweet and slightly warm, mainly in the spleen and lungs. (补 毒 阳阳) to see the aura and lift up the stamina (气气 阳阳) action, tightly to prevent the sweat from the hard-marked (表 表 汗 汗) action, skin and boil, Pus) action, water metabolism to smooth out the treatment of edema and water swallowing (减 水 退肿) and is used in various diseases of children. Symptoms of the spleen due to weakness of the face is white or yellowish, symptoms of limb bloating helplessness, feces are used for dilute symptoms. It is also used when symptoms such as hematemesis, hemorrhage, subcutaneous hemorrhage, and uterine bleeding appear when the weakness is weak and the blood vessel is weakened. It is effective for symptoms such as gastrointestinal irritation, dehydration, long-term dehydration, and drowsiness because it has an energy-raising effect. It is weak, and the face is yellow and dizzy. It is hard to speak, it is hard to talk, it is used for the symptom that the chest swells and the sleep can not be achieved. In addition, it is used when the skin rash is not healed for a long time, and it is known to be used for skin paralysis and sensory paralysis due to hemorrhagic disorder without excessive symptom and energy that can not be excreted in the body due to excessive power.

Eucommia ulmoides is a bark of the mulberry tree belonging to the mulberry tree. It is generally called 'ponzu', and has a sweet, slightly pungent flavor and is warm and non-toxic. It is mainly used for liver and kidney. Liver and kidney function, the ability to strengthen muscles and skeleton, and the ability to protect the fetus during pregnancy. Lumbago caused by lack of function of the gestational sac, and the symptoms of knee flexion and weakness, while strengthening the elasticity of the muscles to promote bone formation. In addition, strengthening the function of gestational esophagus, lower abdomen, lower abdomen cold, urinary symptoms are often used to improve the shrinking power of the bladder. It is effective to prevent premature anxiety, uterine bleeding or miscarriage during pregnancy caused by fragile liver and kidneys. It also controls hypertension due to gangrene deficiency. The pharmacological effects of diapause are directly expanding to the vascular smooth muscle, lowering the blood pressure, anti-aging, enhancing the cholesterol level in the blood, and the leaves of the diapause increase the coronary artery blood flow. It enhances the adrenocortical hormone of animal, shows anti-inflammatory action, controls sedative analgesic action and body immune function, and suppresses and enhances cell immunity. It is known to shorten bleeding time and also to diuretic effect.

Udam is a small gallbladder containing deoxycholic acid (UDCA) more than other gall bladder. Deoxycholic acid is helpful in dissolving and releasing gallstones, and it is also used for such purposes because of its ability to inhibit the acne virus.

 It is known that silkworm is effective for silkworm excretion, diabetes, cell regeneration, and intestinal cleansing.

Injin is an effective drug for prevention of cancer because it has a high content of vitamin A, plays an important role in inhibiting the production and activity of carcinogens, and has a very strong inhibitory action of serum lipids and is a therapeutic agent for preventing arteriosclerosis and hypertension to be. According to the data recorded in Donguibogam, it is known that, in contrast to mugwort, japonica has an excellent effect on jaundice, hepatitis, fatty liver, liver disease, liver cirrhosis, liver cancer, hepatitis B, gastritis, gastrointestinal diseases, constipation, intestinal diseases and women's diseases. Vitamin C is also beneficial to the cold and is known to be effective in the summer heat.

Seomok-tae is a type of black bean, whose skin is black and its size is much smaller than that of black bean. Diabetes, hypertension, arteriosclerosis, heart disease, and various adult diseases. It is known that it has excellent detoxifying ability and has the effect of rapidly recovering destroyed human tissues into the body and releasing various toxicities.

The white mangam is used for various symptoms in one room, and it has a good effect especially in controlling the winds. In addition, it is known to have sleep-promoting action and anti-convulsive action.

Trichosanthes kirilowii MAXIM has long been known as a "covenant of diabetes" because it eliminates thirst due to diabetes. It has efficacy to lower the chest (phlegm), eliminates the pus and stimulates the energy, regulates various inflammatory diseases, and exerts an action to activate the body tissue. It may be heard when jaundice is used when the face and body are yellow, and the lips and mouth dry. In addition, it is effective when it feels like mastitis excellent and mastitis feeling. In some cases, it was formerly used for miscarriage, used to treat symptoms such as reptilia, and is also known to be used for uterine cancer.

Pleuropterus Multflorus is a vinegrow called 'Shark Vine' or 'Pusuguri' which is widely grown in various mountainous areas of Korea. It is said that this grass root is dried and crushed, It is said to be a plant that can be used as a substitute for ginseng. It is said to be a good health food because it has excellent efficacy on nutritional, blood, interpolation, thirst, and diabetes, and improves the fragility and vitality.

The nature of 寒水 石 is garage [寒], the taste is spicy [甘] is sweet, and there is no poison. The heat in Chapter 5, the fever in the stomach, the heat in the body, the stuffiness and sickness in the body, the hot burning in the skin, etc., stop the thirst and swell. It is also called 'coagulant stone' or 'keokseok', and its light is similar to mica and it is good to bend well, which is the regular season of salt.

Ginseng (ginseng) has anticancer effect, it shows a refreshing effect, it does not dry the body, it works to produce blood. The property is a little warm, tasty, a bit spicy, not poisonous. It is known that the non-specific immunity of the living body and the adaptability to various stresses are enhanced, and the normal function is controlled by controlling the dysfunction. It is effective to prevent fatigue, makes the brain and muscles active. So it is good for those who do not get up well in the morning. It also helps to heal diabetes by making syringes, and helps to stop asthma by showing lung aura. It also strengthens the stomach, heals diarrhea, removes toxicity, and heals the wound. However, it is known that it should be noted that it may be poisonous to a person who has a lot of body heat, a person who often sheds nosebleeds or blood, or a person who is stiff with a high blood pressure and does not fit the constitution.

The term "egg shell" means "egg shell," and is not limited to the egg shells of birds such as eggs or sparrow eggs, and may be used as it is or as it is ground. Is used in combination with other herbal ingredients in the present invention to exhibit anti-diabetic efficacy.

The herbal composition according to the present invention is characterized in that it contains 9-17 parts by weight of hwanggi, 9-20 parts by weight of pectin, 10-20 parts by weight of ginseng, 7 to 13 parts by weight, and sewage, marine ginseng, ginseng and ramp are each contained in an amount of 2 to 4 parts by weight. All of these ingredients are meant to be in the form of conventional dry pharmaceutical formulations used in the manufacture of herbal medicines. More preferably, about 20 to about 20 parts by weight of prawn meat is contained in an amount of about 11.7 to about 14.3 parts by weight, and about 10 to about 10 parts by weight of liquorice, ginger berry, mungmudong, To 11.0 parts by weight, and sewage, marine ginseng, ginseng and lran are contained in an amount of 2.7 to 3.3 parts by weight each. When each of the above components is out of the upper and lower limit ranges, the effect of restoring the cardiac function weakening phenomenon due to the vasoconstriction symptoms of diabetic patients is not so large.

The herbal composition of the present invention having the above composition can be subjected to hot water extraction by conventional methods to obtain a hot water extract. The extract or the powdery dry extract obtained by lyophilization can be used for the prevention and treatment of diabetes and complications caused thereby have. Most preferably, the hot water extraction is carried out at a temperature of 100 to 125 DEG C for 1 to 3 hours.

In addition, the herbal composition of the present invention having the above-mentioned composition can be extracted in a conventional manner by using at least one solvent selected from the group consisting of water, an alcohol having 1 to 5 carbon atoms or an aqueous solution of an alcohol, Or a powdery dry extract obtained by lyophilizing the same may be used for the prevention and treatment of diabetes mellitus and its complications. As the solvent, an aqueous solution of ethanol is preferable.

In addition, the herbal composition of the present invention is not particularly limited as far as it is prepared by including the above-mentioned ingredients, and may have a formulation such as a powder, a bath liquid, a pill, and the like.

Hereinafter, the present invention will be described in detail with reference to Preparation Examples and Examples, but the present invention is not limited thereto.

&Lt; Preparation Example 1 >

100g each of dried prawns, 200g of prawns, 130g of prawns, licorice, gigobyphi, mungmudong, tea, golden, Baekbokryeong, mulberry, To prepare a mixture thereof.

&Lt; Preparation Example 2 >

500 g of the mixture of Preparation Example 1 was added to a flask containing 1 L of tertiary distilled water and then subjected to hot water extraction at 100 DEG C for 2 hours to obtain a hot water extract solution. The collected hot water extract was filtered with gauze, and the filtrate was concentrated at 45 ° C and 1 atm using a vacuum filter (Eyela, Japan), and freeze-dried according to ordinary conditions using a freeze dryer to obtain a hot water 130 g of dried extract was obtained.

&Lt; Preparation Example 3 > Alcohol extract of herbal composition

2 L of an aqueous ethanol solution was added to 1 kg of the mixture of Preparation Example 1, and then the mixture was extracted twice with ultrasonic waves for 15 minutes using a conventional ultrasonic processor to obtain an extract, thereby obtaining 3 L of an alcohol extract. The collected extract was filtered with a filter paper and concentrated by a vacuum filter (Eyela, Japan) at 45 ° C and 1 atm, and then lyophilized under ordinary conditions to obtain 90 g of dried powdery alcohol extract.

&Lt; Test Example 1 > Acute toxicity test

(1) oral administration

Fifteen specimens of Sprague-Dawley rats (specific experimental pathogen-free, SPF) of about 160 g were cultivated at a temperature of 23 ± 1 ° C and a humidity of 50 ± 10% ) Were sterilized and sterilized for 5 days in a cage (kerosene, kyunhyang university rearing indoor immunization clean room) without pathogen, and sterilized with an experimental animal feed One well of distilled water was supplied and fed. Thereafter, 5 mice per group were divided into 3 groups. The extract prepared in Preparation Example 2 was suspended in a 0.5% methylcellulose solution and then orally administered to Group 3 at different amounts, 5 g / kg, 10 g / kg and 20 g / kg once. The same experiment was repeated by replacing the extract of Preparation Example 2 with the extract prepared in Preparation Example 3.

(2) Toxicity experiments and results

Thereafter, the mortality, clinical symptoms, and weight changes of the animal were observed according to the usual method, and hematological examination and blood biochemical test were performed, and autopsy was performed to observe whether or not the abdominal organs and thoracic organs were abnormal. As a result, none of the animals treated with the extracts of Preparation Examples 2 and 3 exhibited any specific clinical symptoms or died, and toxic changes were observed even in weight change, hematological test, blood biochemical test, Not observed. Since the extract of the present invention did not exhibit any toxic change until 20 g / kg of the extract was administered to rats, it was judged that the extract was safe because the oral LD50 was 20 g / kg or more.

As a result of the same toxicity test by intraperitoneal administration of the same extract to rats according to the usual method, the LD50 of 20 g / kg or more by intraperitoneal administration was judged to be a safe substance .

<Examples>

(1) Classification of the group

90 rats of about 160-165 g of wister male rats (Central Experimental Animals Co., Ltd., Korea) were maintained at a constant temperature (23 1 캜) and humidity (50 10% Five rabbits per cage were sterilized for one week in a breeding room (Kyunghee University College of Oriental Medicine, Breeding and Interior Immunization Room) and fed with sterilized distilled water and feed for laboratory animals (Korea) . Then, 30 rats per group were divided into 3 groups.

(2) Preparation of herbal composition and streptozotocin

Streptozotocin (STZ) was also prepared in advance to prepare the extracts prepared in Preparation Examples 2 and 3 for administration to the subject group of the present invention, and to administer them to the subject group of the Comparative Example and the subject group of the Example . Streptozotocin is selectively used to kill beta cells of the pancreas and to prevent insulin production and secretion, thereby causing diabetes.

(3) Preparation of control, experimental and comparative groups

Streptozotocin was not administered to rats in one of the three groups prepared in (1) above. They are normal rats without diabetes and are used as a control group.

The herbal composition of (2) was orally administered to rats of the other groups prepared in the above (1) for 3 days twice a day at a dose of 4 g / kg from 3 days prior to the administration of screptozotocin peritoneum , Streptozotocin was intraperitoneally administered at a dose of 60 mg / kg, and then the herbal composition of (2) was orally administered at a dose of 4 g / kg twice a day for 40 days. These are used as an experimental group as diabetic mice to which the herbal composition of the present invention is administered before and after diabetes induction.

On the other hand, in another group of rats prepared in (1), streptozotocin was intraperitoneally administered at a dose of 60 mg / kg once at the same time as the experimental group. They were used as a comparative group as a diabetic rats that induced diabetes but did not administer the herbal composition of the present invention.

&Lt; Test Example 2 >

The control group, the experimental group, and the control group prepared in the Example were divided into 7 groups of 10 days, 7 days of 20 days, 7 days of 30 days, and 10 days of the first administration of streptozotocin, The final weight and heart rate at 10 days, 20 days, 30 days, and 40 days after the passage of time were divided into nine groups of 40 days, and the results were averaged, Respectively.

Initial weight (g) Final weight (g) Heart rate (bpm) 10 days past Control group 161 ± 8 187 ± 9 362 ± 8 Experimental group 165 ± 8 180 ± 10 353 ± 11 Comparative group 163 ± 7 171 ± 12 345 ± 13 20 days past Control group 165 ± 10 243 ± 11 ^* 369 ± 9 Experimental group 160 ± 17 223 ± 10 ^* 357 ± 12 Comparative group 159 ± 7 184 ± 7 343 ± 10 30 days past Control group 160 ± 7 267 ± 13 ^* 354 ± 15 Experimental group 164 ± 17 241 ± 18 ^* 333 ± 15 Comparative group 163 ± 7 191 ± 8 314 ± 18 40 days past Control group 161 ± 10 305 ± 11 ^* 348 ± 16 Experimental group 168 ± 15 267 ± 10 ^* 317 ± 15 Comparative group 161 ± 6 214 ± 25 327 ± 11 * Is P <0.05 by student t- test when compared to the values of each comparative group

As can be seen from the above Table 1, the body weight gain rate of the comparative group is significantly lower than that of the control group, and it can be predicted that diabetes has been induced. Such phenomenon became more prominent as the number of days elapsed. On the other hand, the weight gain rate of the experimental group was slightly slower than that of the control group, and there was a significant difference when compared with the comparison group. From this, it can be seen that the test group administered with the extract of the present invention has an antidiabetic effect.

&Lt; Test Example 3 > Plasma Blood Glucose Change

Plasma glucose levels at 10 days, 20 days, 30 days and 40 days after the first administration of streptozotocin in the control, experimental and comparison groups subdivided in Test Example 2 were measured by the usual method And the average of the results are shown in Table 2 and FIG.

Plasma Blood Sugar Level (Unit: mg / ㎗) 10 days past Control group 128.8 (17.5) Experimental group 209.8 (15.7) Comparative group 281.7 (42.8) 20 days past Control group 138.8 (18.1) Experimental group 327.3 ^* (23.6) Comparative group 413.9 (18.3) 30 days past Control group 131.5 (14.7) Experimental group 359.2 ^* (28.3) Comparative group 463.7 (32.3) 40 days past Control group 140.3 (25.8) Experimental group 356.1 ^* (27.5) Comparative group 453.8 (35.7) * Is P <0.01, and the numbers in parentheses are standard error (SE)

As shown in Table 2 and FIG. 1, the blood glucose level of the control group was significantly increased compared to that of the control group, and the glucose level measured at the time of 10 days showing the lowest blood glucose level was 280 mg / Can be predicted. On the other hand, the experimental group shows a significantly decreased blood glucose level as compared with the comparison group. From this, it can be seen that the extract of the present invention has an antidiabetic effect.

Test Example 4 Mean arterial pressure change

The mean arterial pressure (MAP) at 10 days, 20 days, 30 days, and 40 days after the first administration of streptozotocin was measured in the control group, the experimental group, and the control group subdivided in Test Example 2 And the average of the results are shown in Table 3 and FIG. 2, respectively.

Mean arterial pressure (in mmHg) 10 days past Control group 118.3 (5.3) Experimental group 113.6 (7.0) Comparative group 104.7 (4.3) 20 days past Control group 120.8 (4.6) Experimental group 110.8 (8.2) Comparative group 101.6 (7.8) 30 days past Control group 115.4 (3.9) Experimental group 112.9 ^* (5.8) Comparative group 94.1 (4.8) 40 days past Control group 121.7 (7.6) Experimental group 118.6 ^* (7.8) Comparative group 91.5 (8.3) * Is P <0.01, and the numbers in parentheses are standard error (SE)

As shown in Table 3 and FIG. 2, the blood pressure of the control group was significantly decreased as compared with the control group, and the blood pressure was further decreased as the number of days elapsed. And vasoconstriction caused by diabetes, and the like. On the other hand, the blood pressure of the experimental group is higher than that of the control group, and the effect is similar to that of the control group. From this, it can be seen that the extract of the present invention has an effect of restoring blood pressure reduction caused by diabetes.

Test Example 5 Shrinkage and relaxation change

(1) Preparation of physiological saline (PSS)

Physiological saline having a composition of 136.9 mM NaCl, 5.4 mM KCl, 1.5 mM CaCl ₂ , 1.0 mM MgCl ₂ , 23.8 mM NaHCO _3, 5.5 mM glucose and 0.01 mM EDTA was prepared.

(2) Preparation of aortic specimen and measurement of relaxation

Nine of the rats in the control group, the experimental group, and the control group, which had been on the day of the first administration of streptozotocin, were swallowed in the stable state and falcified. After the bleeding, the left thoracic incision was made and the aorta was carefully removed. The extracted aorta was removed from the dish containing physiological saline prepared in (1), and the aorta was separated under a microscope to make a ring specimen having a width of about 2 mm. At this time, the experimental conditions of physiological saline were 95% O ₂ And 5% CO _{2. Then,} the mixture was maintained at pH 7.4 with a mixed gas of 95% O ₂ and 5% CO ₂ at 37 ° C during the experiment. One end of the ring was fixed with a fixer and the other end was connected to a force transducer (Force Displacement Transducer, FT03, GRASS), transferred to a tissue sample container (bath, 5 ml) and recorded with a physiograph.

The aortic ring surface was rested for 1 hour with a resting tension of about 30 minutes and then repeatedly treated with 25 mM isotonic KCl and physiological saline solution.

(3) Test subjects - Changes in relaxation response according to the concentration of extract administered after shrinkage of KCl

The aorta of each group extracted in the above (2) was subjected to the shrinkage reaction to 70 mM KCl, and after ethanol treatment, the relaxation rate of the aorta according to the concentration of the extract was measured according to a conventional method And the average of the results are shown in Table 4 and FIG. At this time, the extract of 1 ~ 300 ㎍ / ㎖ was injected into the aorta using a syringe, and the relaxation value was recorded as 100% (relaxation 0%) for KCl. The aortic relaxation rate of diabetic-induced animals is usually significantly lower than the aortic relaxation rate of normal animals.

Relaxation rate (%) Extract dose log [extract] (unit: mu g / ml) One 3 10 30 100 300 Control group 0.98 (2.53) 1.75 (1.78) 3.68 (5.13) 15.87 (3.11) 58.45 (4.87) 51.55 (5.78) Experimental group 1.1 (1.83) 2.7 (3.1) 3.8 (3.8) 8.3 (3.8) 37.4 ^* (4.17) 35.8 ^* (3.5) Comparative group 2.1 (3.76) 1.7 (2.53) 2.75 (3.51) 4.87 (5.33) 8.78 (6.38) 12.65 (5.37) * Is P <0.01, and the numbers in parentheses are standard error (SE)

As shown in Table 4 and FIG. 3, the aortic relaxation rate of each group has a common point that the dose does not increase from 100 μg / ml or more, and the maximum relaxation rate of the experimental group and the comparative group Which is a significant difference when compared. Also, the relaxation rate of the comparative group shows a great difference compared to the relaxation rate of the normal control group. From this, it can be seen that the extract of the present invention can prevent and treat the occurrence of diabetes complications such as hyperlipidemia and atherosclerosis which may occur due to contraction of blood vessels such as aorta and the like due to ease of deposition of platelets and cholesterol by diabetes, .

(4) Test subjects - Shrinkage response to norepinephrine concentration

The aorta of each group extracted in the above (2), 100 ㎍ / ml of the ethanol extract of the present invention was bombarded 1 minute before the start of the experiment, and the extract continued to remain in the bath during the experiment . The norepinephrine, which is a vasoconstrictor, was treated and the contraction ratio of the aorta according to the administration concentration was measured according to a usual method, and the results are averaged as shown in Table 5 and FIG. Norepinephrine at a concentration of 0.003-30 μM was injected into the aorta using a syringe and the contraction rate was recorded as 100% for KCl based on the baseline. The aortic contraction rate of diabetic-induced animals is usually significantly higher than the aortic contraction rate of normal animals.

Shrinkage (%) Norepinephrine dose log [norepinephrine] (unit: μM) 0.003 0.01 0.03 0.1 0.3 One 3 10 30 Control group 0.8 (2.58) 2.58 (6.16) 10.55 (5.87) 48.51 (5.63) 75.78 (7.6) 81.43 (7.89) 85.37 (9.15) 102.78 (8.57) 108.78 (7.27) Experimental group 1.1383 (0.88) 3.07 (3.38) 9.15 (4.72) 35.31 ^* (7.16) 60.68 ^* (5.83) 73.1 ^* (8.32) 75.05 ^* (5.78) 83.71 (7.27) 87.23 (8.2) Comparative group 1.75 (3.78) 3.77 (1.65) 5.46 (3.85) 15.56 (6.07) 35.18 (7.39) 42.96 (5.78) 53.97 (8.78) 71.71 (5.35) 83.62 (8.17) * Is P <0.01, and the numbers in parentheses are standard error (SE)

As can be seen in Table 5 and FIG. 4, the aortic contraction ratio of each group is not expected to increase from 30 μM or more, and in the experimental group, the concentration of 0.1 to 3 μM, And decreased shrinkage. In addition, the shrinkage of the control group was significantly different from the shrinkage of the control group. From this, it can be seen that the extract of the present invention can prevent and treat the occurrence of diabetic complications such as hyperlipemia and arteriosclerosis which may occur due to the blood vessels of the aorta and the like being easily relaxed due to easy platelet and cholesterol deposition by the diabetes .

(5) Subjects - Changes in relaxation response according to acetylcholine administration concentration

The aorta of each group extracted in the above (2) was subjected to the battle 1 minute before the start of the experiment with 100 μg / ml of the ethanol extract, and the extract remained in the bath during the experiment. After stabilization with physiological saline, acetylcholine, a blood vessel relaxant, was administered into the aorta using a syringe, and the relaxation rate of the aorta according to the administration concentration was measured according to a conventional method. Shown in Table 6 and Fig. Acetylcholine was administered at a concentration of 0.003 to 30 μM, and relaxation rates were recorded for each concentration with 100% relaxation (0% relaxation) to KCl based on the baseline. The aortic relaxation rate of diabetic-induced animals is usually significantly lower than the aortic relaxation rate of normal animals.

Relaxation rate (%) Acetylcholine dose log [acetylcholine] (unit: μM) 0.003 0.01 0.03 0.1 0.3 One 3 10 30 Control group 0 (0) 0 (5.01) 11.25 (3.17) 38.99 (5.65) 52.46 (4.07) 63.47 (5.11) 72.13 (6.15) 93.83 (4.48) 97.58 (5.37) Experimental group 0 (0) 0 (0) 8.37 (3.14) 31.58 ^* (5.1) 38.38 ^* (4.84) 52.18 ^* (3.18) 61.51 ^* (5.15) 78.18 ^* (6.31) 83.91 ^* (4.87) Comparative group 0 (0) 0 (0) 5.16 (1.53) 13.58 (5.27) 23.27 (3.17) 32.15 (4.28) 43.16 (7.63) 55.18 (5.48) 67.38 (7.11) * Is P <0.01, and the numbers in parentheses are standard error (SE)

As can be seen in Table 6 and FIG. 5, the experimental group exhibited a significant relaxation rate increase at a treatment concentration of 0.1-30 μM as compared to the control group. In addition, the relaxation rate of the control group was significantly different from the relaxation rate of the control group. From this, it can be seen that the extract of the present invention can prevent and treat the occurrence of diabetic complications such as hyperlipemia and arteriosclerosis which may occur due to the blood vessels of the aorta and the like being easily relaxed due to easy platelet and cholesterol deposition by the diabetes .

According to the present invention, diabetes and its complications can be prevented and treated by administering the herbal composition of the present invention. In particular, it is possible to effectively inhibit the phenomenon of vascular disorders belonging to the main symptom of diabetes so that vessels such as the aorta are contracted and not relaxed The occurrence of diabetic complications such as hyperlipemia and arteriosclerosis that can occur without the occurrence of the disease can be effectively prevented and treated.

While the invention has been shown and described with reference to certain exemplary embodiments thereof, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims.

Claims (6)

7 to 13 parts by weight of licorice, 7 to 13 parts by weight of ginger, 7 to 13 parts by weight of ginger root, 7 to 13 parts by weight of ginger, 7 to 13 parts by weight of gold, 7 to 13 parts by weight of Baekbok- 7 to 13 parts by weight of starch, 7 to 13 parts by weight of starch, 7 to 13 parts by weight of starch, 7 to 13 parts by weight of starch, 7 to 13 parts by weight of starch, 7 to 13 parts by weight of starch, To 3 parts by weight of ginseng, 2 to 4 parts by weight of ginseng, 2 to 4 parts by weight of ginseng, 2 to 4 parts by weight of ginseng, 2 to 4 parts by weight of ginseng, 2 to 4 parts by weight of ginseng and 2 to 4 parts by weight of rhamnia. Composition. 9. The method according to claim 1, wherein 9.0 to 11.0 parts by weight of licorice, 9.0 to 11.0 parts by weight of gingerol, 9.0 to 11.0 parts by weight of ginger root, 9.0 to 11.0 parts by weight of ginger, 9.0 to 11.0 parts by weight of golden, 11.0 to 11.0 parts by weight of sulfur, 9.0 to 11.0 parts by weight of manganese, 9.0 to 11.0 parts by weight of humectant, 9.0 to 11.0 parts by weight of silane, 9.0 to 11.0 parts by weight of silane, 9.0 to 11.0 parts by weight of silane, 9.0 to 11.0 parts by weight, , 2.7 to 3.3 parts by weight of ginseng, 2.7 to 3.3 parts by weight of ginseng and 2.7 to 3.3 parts by weight of Ranma. &Lt; / RTI &gt; A hot-water extract obtained by subjecting the herbal composition of claim 1 or 2 to hot water extraction. A powdery extract obtained by freeze-drying the hot-water extract solution of claim 3. A solvent extract obtained by extracting the herbal composition of claim 1 or 2 with at least one solvent selected from the group consisting of water, an alcohol having 1 to 5 carbon atoms, or an aqueous solution of an alcohol. A powdery extract obtained by lyophilizing the solvent extract of claim 5.

Images