KR20060107939A - 국내 돼지에 존재하는 내인성 레트로바이러스의 엔밸로프유전자 - Google Patents

국내 돼지에 존재하는 내인성 레트로바이러스의 엔밸로프유전자 Download PDF

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KR20060107939A
KR20060107939A KR1020060032581A KR20060032581A KR20060107939A KR 20060107939 A KR20060107939 A KR 20060107939A KR 1020060032581 A KR1020060032581 A KR 1020060032581A KR 20060032581 A KR20060032581 A KR 20060032581A KR 20060107939 A KR20060107939 A KR 20060107939A
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김영봉
박홍양
이동희
이정은
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Abstract

본 발명은 국내 돼지 내인성 레트로바이러스의 신규한 env 뉴클레오타이드, 국내 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커 그리고 국내 돼지 내인성 레트로바이러스의 동정방법에 관한 것이다. 본 발명의 env 유전자는 국내 돼지 내인성 레트로바이러스에 대하여 특이적으로 발현되는 것이며, env 유전자 내의 유전적 마커를 이용하는 경우에는 국내 돼지 내인성 레트로바이러스를 정확하게 동정할 수 있다.
돼지 내인성 레트로 바이러스, PERV, 이종간 장기 이식, 엔벨로프 단백질, env

Description

국내 돼지에 존재하는 내인성 레트로바이러스의 엔밸로프 유전자{Envelope Genes of Porcine Endogenous Retroviruses from Domestic Pigs in Korea}
도 1a 및 1b는 국내 돼지로부터 얻은 돼지 내인성 레트로바이러스 클래스 A (PERV-A) env 단백질의 아미노산 서열의 얼라인먼트이다. 클로닝된 env 단백질의 gp70(envelope surface glycoprotein)과 p15E의 부분 서열을 얼라인먼트 하였다.
도 2는 국내 돼지로부터 얻은 돼지 내인성 레트로바이러스 클래스 B(PERV-B) env 단백질의 아미노산 서열의 얼라인먼트이다. 클로닝된 env 단백질의 gp70(envelope surface glycoprotein)과 p15E의 부분 서열을 얼라인먼트 하였다. PERV-A의 경우, 기준 바이러스주로서 Y12238 및 AY288779를 이용하여 얼라인먼트 하였고(도 1), PERV-B의 경우, 기준 바이러스주로서 Y12239 및 AJ133818을 이용하여 얼라인먼트 하였다(도 2). B 클론은 버크셔-유래이고, D 클론은 듀로크-유래이며, L 클론은 랜드레이스-유래이고, Y 클론은 요크셔-유래이다. 음영 박스 내의 문자는 엔벨로프 표면 당단백질의 당화 위치를 나타낸다. 솔리드 라인 박스의 문자는 gp70과 p15E를 분리하는 절단 위치를 나타낸다. 대쉬는 동일한 아미노산 서열을 나타낸다.
도 3은 국내 돼지로부터 얻은 PERV env 뉴클레오타이드 서열에 기초한 이웃- 결합 트리(neighbor-joining tree)이다. 트리는 본 발명의 env-서열에 기초하여 Galtier & Gouy 방법(1995)에 따라 Treecon ver 1.3b을 이용하여 작성하였다. 노드의 숫자는 100개의 리샘플링 데이터세트의 부트스트랩(bootstrap) 값을 나타낸다. PERV-A(A312521, AF507940 및 Y12238), PERV-B(AJ288587, AJ288589 및 Y12239), PERV-C(AF038600 및 AF402662) 및 PERV-E(AF356697)은 기준 바이러스주로 이용되었다.
도 4는 국내 돼지의 PERV env 유전자 사이의 뉴클레오타이드 상동성 및 아미노산 서열 상동성(%)을 보여주는 표이다.
도 5a는 본 발명의 PERV-A 또는 PERV-B 특이적 프라이머를 이용하여 실시된 증폭 결과를 보여주는 사진이다.
도 5b는 본 발명의 프라이머를 이용하여 실시된 RFLP(restriction fragment length polymorphism) 분석 결과를 보여주는 사진이다.
본 발명은 국내 돼지 내인성 레트로바이러스의 env(엔벨로프) 단백질 및 유전자에 관한 것이다.
장기 이식 기술의 발달에 의하여 이식이 필요한 환자의 증가에 비해 필요한 인간 장기의 절대적 부족현상으로 인하여 돼지의 장기, 조직 또는 세포를 인간에게 이식하는 이종간 장기 이식이 많은 관심 하에 활발히 연구가 진행되고 있다(Dorling 등, 2002; White와 Nicholson, 1999). 최근 조류 인플루엔자, SARS, AIDS 등 동물유래의 바이러스 전염병이 문제화 되면서, 돼지를 이용한 인간으로의 장기 이식 또한 이러한 문제점이 크게 부각 되었다(Magre 등, 2003). 비록 무균돼지의 사육을 통하여 대부분의 외인성 병원균은 제거가 가능하지만, 돼지 유래의 내인성 병원균은 제거가 거의 불가능 하다(Swindle, 1998).
돼지 내인성 레트로바이러스(porcine endogenous retroviruses: PERVs)는 가장 대표적인 돼지의 내인성 바이러스의 하나로서, 1970년에 처음으로 보고 되었다(Breese, 1970). Retroviridae 과 Gammaretrovirus 속으로 분류 되며(Klymiuk 등, 2002), 염기서열 및 아미노산 서열, 형태학적으로 백혈병이나 면역 결핍증을 일으키는 쥐 류케미아 바이러스 및 고양이 류케미아 바이러스와 같이 γ-레트로바이러스에 속하는 것으로 보고 되었다(Tacke 등, 2000). PERVs는 돼지 내에서는 어떠한 병리적 증상을 나타내지 않으며, 돼지 지놈 안에 약 13 종의 PERVs가 존재하는 것으로 보고 되었으며 대부분 프로바이러스는 유전자 결실 또는 돌연변이로 인해 실제 바이러스를 만들 수 없는 형태로 존재하고 있다(Patience 등, 1997).
이들 중 γ1 패밀리 내 서브패밀리 A, B 및 C 세 클래스가 바이러스를 만들고 감염능이 있는 것으로 알려져 있다(Magre 등, 2003). 많은 인간 세포는 PERV-A와 PERV-B에 특이적인 수용체를 가지고 있으며 PERV-C에 특이적인 수용체는 HT1080 세포주를 제외하고 아직까지 보고된 바 없다(Mollnes 등, 1999; Patience 등, 1997). 가장 최근에 발생한 것으로 보이는 PERV-C는 PERV-A와 재조합 반응을 일으키면서 그 감염성이 500배나 증가하는 것으로 밝혀져 주목을 받았다(Harrison 등, 2004).
감염성 PERVs는 in vitro 상에서는 넓은 범위의 감염성을 보이지만 in vivo 상에서는 면역능력을 결핍시킨 쥐 외에 아직까지 알려진 예는 없다(Heneine 등, 1998; Patience 등, 1998). 그러나 실제 장기 이식을 받는 환자는 지속적으로 면역 억제제를 투여하기 때문에 PERV에 대한 위험성이 더욱 부각된다. 또한 사람의 유전자 안에도 PERVs와 유사한 인간 내인성 레트로바이러스(HERVs)가 존재하고 있으며, 이는 분류상 PERV와 비슷한 γ 레트로바이러스에 속한다. 현재까지 밝혀진 HERVs중 실제 감염성이 있는 바이러스는 알려져 있지 않으나, 이종간 장기 이식에 의해 PERVs가 HERVs와 재조합을 일으켜 새로운 형태의 바이러스가 생성될 가능성은 무시될 수 없으며, AIDS를 유발하는 인간 면역결핍 바이러스(HIV)와 같이 인류에 재앙을 가져올 수 있는 위험성을 내포하고 있다(Klymiuk 등, 2002, 2003).
본 발명자들은 국내 돼지 내인성 레트로바이러스의 지놈 연구의 일환으로써, env 유전자에 대한 연구를 하였고 결국 국내 돼지 내인성 레트로바이러스에 특이적인 env 유전자를 규명함으로써 본 발명을 완성하게 되었다.
따라서, 본 발명의 목적은 돼지 내인성 레트로바이러스의 env 핵산 분자를 제공하는 데 있다.
본 발명의 다른 목적은 국내 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공하는 데 있다.
본 발명의 또 다른 목적은 국내 돼지 내인성 레트로바이러스의 동정방법을 제공하는 데 있다.
본 발명의 다른 목적은 국내 돼지 PERV-A와 B를 구별하여 동정할 수 있는 프라이머를 제공하는 데 있다.
본 발명의 다른 목적 및 이점은 하기의 발명의 상세한 설명, 청구범위 및 도면에 의해 보다 명확하게 된다.
본 발명의 일 양태에 따르면, 본 발명은 서열목록 제1서열 내지 제92서열로 구성된 군으로부터 선택되는 무균 돼지 내인성 레트로바이러스의 env 단백질을 코딩하는 뉴클레오타이드 서열을 포함하는 env 뉴클레오타이드를 제공한다.
본 발명의 env 뉴클레오타이드는 국내 돼지에 존재하는 돼지 내인성 레트로바이러스에서 발현되는 신규한 서열의 뉴클레오타이드이다. 본 명세서에서, 용어“국내 돼지(domestic pigs)”는 국내에서 육종, 번식 및 생산되는 돼지를 의미한다. 바람직하게는, 국내에서 사육되는 요크셔(Yorkshire) 품종, 랜드레이스(Landrace) 품종, 듀로크(Duroc) 품종 및 버크셔(Berkshire) 품종의 돼지를 의미 한다.
한편, 국내 돼지 내인성 레트로바이러스를 연구하는 데 있어서, 본 발명에서 env 단백질 및 유전자를 타깃으로 설정한 이유는, env 단백질의 표면 당단백질 부분이 돼지 내인성 레트로바이러스(porcine endogenous retrovirus: PERV)의 서브그룹(클래스) 및 호스트의 범위와 특이성을 결정할 뿐만 아니라 중화항체(neutralizing antibody) 생성에 필수적인 항원의 역할을 하기 때문이다.
본 발명의 env 뉴클레오타이드, 즉 엔벨로프를 코딩하는 뉴클레오타이드는 돼지 내인성 레트로바이러스, 바람직하게는 국내 돼지 내인성 레트로바이러스, 보다 바람직하게는 국내 돼지 내인성 레트로바이러스로서 γ-레트로바이러스, 보다 더 바람직하게는 국내 돼지 내인성 레트로바이러스로서 γ1-레트로바이러스, 가장 바람직하게는 국내 돼지 내인성 레트로바이러스로서 γ1-레트로바이러스의 서브그룹(클래스) A 또는 B에 속하는 레트로바이러스의 엔벨로프 뉴클레오타이드이다.
본 발명의 env 뉴클레오타이드는 상술한 국내 돼지 내인성 레트로바이러스에서 특이적으로 발현되는 뉴클레오타이드이다.
서열목록 제1서열 내지 제92서열에 기재된 본 발명의 env 뉴클레오타이드 중 30%에 해당되는 뉴클레오타이드 서열은 올바른 오픈 리딩 프레임을 갖는다. 이러한 올바른 오픈 리딩 프레임을 갖는 env 뉴클레오타이드가 있는 PERV는 이종간 장기 이식 시 감염의 위험성이 있다. 따라서, 서열목록 제1서열 내지 제92서열에 기재된 본 발명의 env 뉴클레오타이드 중 올바른 오픈 리딩 프레임을 갖는 제3서열, 제7서열, 제9서열, 제11서열, 제12서열, 제17서열, 제23서열, 제25서열, 제27 서열, 제29서열, 제30서열, 제31서열, 제37서열, 제42서열, 제43서열, 제46서열, 제49서열, 제50서열, 제57서열, 제60서열, 제69서열, 제70서열, 제72서열, 제128서열, 제131서열, 제83서열, 제89서열 및 제92서열이 중요한 의미를 갖는다.
본 발명에서 클로닝된 엔벨로프 유전자들은 중화 항체 생산을 통한 PERV 제어에 적용될 수 있다.
본 발명의 또 다른 양태에 따르면, 본 발명은 서열목록 제9서열, 제11서열, 제17서열, 제25서열, 제29서열, 제31서열, 제42서열, 제46서열, 제57서열, 제60서열, 제69서열, 제70서열, 제72서열, 제83서열, 제89서열 또는 제92서열에서 418번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제9서열, 제11서열, 제17서열, 제25서열, 제29서열, 제31서열, 제42서열, 제46서열, 제57서열, 제60서열, 제69서열, 제70서열, 제72서열, 제83서열, 제89서열 또는 제92서열에서 672번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제9서열, 제29서열, 제31서열, 제42서열, 제57서열, 제72서열, 제83서열 또는 제92서열에서 1133번째 뉴 클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제11서열, 제25서열, 제46서열, 제49서열 또는 제60서열에서 917번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제29서열, 제31서열 또는 제83서열에서 46번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제29서열, 제31서열, 제83서열 또는 제89서열에서 56번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제29서열, 제31서열 또는 제83서열에서 140번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제29서열, 제31서열 또 는 제83서열에서 196번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제29서열, 제31서열 또는 제83서열에서 209번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제29서열, 제31서열 또는 제83서열에서 340번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제29서열, 제31서열 또는 제83서열에서 491번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제29서열, 제31서열 또는 제83서열에서 589번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제29서열, 제31서열 또 는 제83서열에서 685번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제29서열, 제31서열 또는 제83서열에서 700번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제11서열, 제17서열 또는 제60서열에서 745번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제29서열, 제31서열 또는 제83서열에서 845번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제29서열, 제31서열 또는 제83서열에서 866번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제29서열, 제31서열 또 는 제83서열에서 874번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제29서열, 제31서열 또는 제83서열에서 886번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제29서열, 제31서열 또는 제83서열에서 995번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제12서열, 제30서열 또는 제43서열에서 28번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제23서열, 제27서열 또는 제50서열에서 829번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명의 다른 양태에 따르면, 본 발명은 서열목록 제12서열, 제30서열 또 는 제43서열에서 902번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커를 제공한다.
본 발명자들은, 종래의 PERV의 env 단백질 또는 유전자에는 없고, 국내 돼지 내인성 레트로바이러스에서 특이적으로 존재하는 아미노산 서열 및 뉴클레오타이드 서열을 발견하였다. 이러한 뉴클레오타이드 서열은 국내 돼지 내인성 레트로바이러스를 동정하는 데 특이한 유전적 마커로서 이용될 수 있다.
상술한 유전자 마커들 중, 418번째 뉴클레오타이드는 “g"로서, 코돈 “gta"의 첫 번째 뉴클레오타이드이며, 이 코돈은 Val을 코딩한다. 종래의 PERV에서 이 코돈은 ”ata"로 되어 있고, “Ile"을 코딩한다. 672번째 뉴클레오타이드는 “g"로서, 코돈 “atg"의 세 번째 뉴클레오타이드이며, 이 코돈은 Met을 코딩한다. 종래의 PERV에서 이 코돈은 “ata"로 되어 있고, ”Ile"을 코딩한다. 1133번째 뉴클레오타이드는 "g"로서, 코돈 "agg"의 세 번째 뉴클레오타이드이며, 이 코돈은 Arg을 코딩한다. 종래의 PERV에서 이 코돈은 "atg"로 되어 있고 “Met"을 코딩한다. 917번째 뉴클레오타이드는 ”a"로서, 코돈 “aac"의 두 번째 뉴클레오타이드이며, 이 코돈은 Asn을 코딩하고, 종래의 PERV에서 이 코돈은 "agc"로 되어 있고 “Ser"을 코딩한다. 46번째 뉴클레오타이드는 ”g"로서, 코돈 “gag"의 첫 번째 뉴클레오타이드이며, 이 코돈은 "Glu"을 코딩하고, 종래의 PERV에서 이 코돈은 "aag"로 되어 있고 “Lys"을 코딩한다.
56번째 뉴클레오타이드는 ”a"로서, 코돈 “aaa"의 첫 번째 뉴클레오타이드 이며, 이 코돈은 "Lys"을 코딩하고, 종래의 PERV에서 이 코돈은 "aga"로 되어 있고 “Arg"을 코딩한다. 140번째 뉴클레오타이드는 ”c"로서, 코돈 “gcg"의 두 번째 뉴클레오타이드이며, 이 코돈은 "Ala"을 코딩하고, 종래의 PERV에서 이 코돈은 "gtg"로 되어 있고 “Val"을 코딩한다. 196번째 뉴클레오타이드는 ”a"로서, 코돈 “agt"의 첫 번째 뉴클레오타이드이며, 이 코돈은 "Ser"을 코딩하고, 종래의 PERV에서 이 코돈은 "ggt"로 되어 있고 “Gly"을 코딩한다. 209번째 뉴클레오타이드는 ”c"로서, 코돈 “act"의 두 번째 뉴클레오타이드이며, 이 코돈은 "Thr"을 코딩하고, 종래의 PERV에서 이 코돈은 "aat"로 되어 있고 “Asn"을 코딩한다. 340번째 뉴클레오타이드는 ”a"로서, 코돈 “aat"의 첫 번째 뉴클레오타이드이며, 이 코돈은 "Asn"을 코딩하고, 종래의 PERV에서 이 코돈은 "tat"로 되어 있고 “Tyr"을 코딩한다. 196번째 뉴클레오타이드는 ”a"로서, 코돈 “agt"의 첫 번째 뉴클레오타이드이며, 이 코돈은 "Ser"을 코딩하고, 종래의 PERV에서 이 코돈은 "ggt"로 되어 있고 “Gly"을 코딩한다.
491번째 뉴클레오타이드는 ”g"로서, 코돈 “agt"의 두 번째 뉴클레오타이드이며, 이 코돈은 "Ser"을 코딩하고, 종래의 PERV에서 이 코돈은 "aat"로 되어 있고 “Asn"을 코딩한다. 589번째 뉴클레오타이드는 ”a"로서, 코돈 “aat"의 첫 번째 뉴클레오타이드이며, 이 코돈은 "Asn"을 코딩하고, 종래의 PERV에서 이 코돈은 "cat"로 되어 있고 “His"을 코딩한다. 685번째 뉴클레오타이드는 ”a"로서, 코돈 “atg"의 첫 번째 뉴클레오타이드이며, 이 코돈은 "Met"을 코딩하고, 종래의 PERV에서 이 코돈은 "gtg"로 되어 있고 “Val"을 코딩한다. 700번째 뉴클레오타 이드는 ”a"로서, 코돈 “act"의 첫 번째 뉴클레오타이드이며, 이 코돈은 "Thr"을 코딩하고, 종래의 PERV에서 이 코돈은 "tct"로 되어 있고 “Ser"을 코딩한다. 745번째 뉴클레오타이드는 ”a"로서, 코돈 “aaa"의 첫 번째 뉴클레오타이드이며, 이 코돈은 "Lys"을 코딩하고, 종래의 PERV에서 이 코돈은 "gaa"로 되어 있고 “Glu"을 코딩한다. 845번째 뉴클레오타이드는 ”t"로서, 코돈 “gtt"의 두 번째 뉴클레오타이드이며, 이 코돈은 "Val"을 코딩하고, 종래의 PERV에서 이 코돈은 "gat"로 되어 있고 “Asp"을 코딩한다. 866번째 뉴클레오타이드는 ”t"로서, 코돈 “tta"의 두 번째 뉴클레오타이드이며, 이 코돈은 "Leu"을 코딩하고, 종래의 PERV에서 이 코돈은 "tca"로 되어 있고 “Ser"을 코딩한다. 874번째 뉴클레오타이드는 ”c"로서, 코돈 “cct"의 첫 번째 뉴클레오타이드이며, 이 코돈은 "Pro"을 코딩하고, 종래의 PERV에서 이 코돈은 "act"로 되어 있고 “Thr"을 코딩한다.
886번째 뉴클레오타이드는 ”t"로서, 코돈 “ttc"의 첫 번째 뉴클레오타이드이며, 이 코돈은 "Phe"을 코딩하고, 종래의 PERV에서 이 코돈은 "atc"로 되어 있고 “Ile"을 코딩한다. 995번째 뉴클레오타이드는 ”t"로서, 코돈 “ttg"의 두 번째 뉴클레오타이드이며, 이 코돈은 "Leu"을 코딩하고, 종래의 PERV에서 이 코돈은 "tcg"로 되어 있고 “Ser"을 코딩한다. 28번째 뉴클레오타이드는 ”g"로서, 코돈 “gtc"의 첫 번째 뉴클레오타이드이며, 이 코돈은 "Val"을 코딩하고, 종래의 PERV에서 이 코돈은 "ctc"로 되어 있고 “Leu"을 코딩한다. 829번째 뉴클레오타이드는 ”a"로서, 코돈 “agt"의 첫 번째 뉴클레오타이드이며, 이 코돈은 "Ser"을 코딩하고, 종래의 PERV에서 이 코돈은 "ggt"로 되어 있고 “Gly"을 코딩한다. 902번 째 뉴클레오타이드는 ”c"로서, 코돈 “ccc"의 두 번째 뉴클레오타이드이며, 이 코돈은 "Pro"을 코딩하고, 종래의 PERV에서 이 코돈은 "ctc"로 되어 있고 “Leu"을 코딩한다.
한편, 본 발명은 상술한 국내 돼지 내인성 레트로바이러스의 env 뉴클레오타이드 서열에서 상술한 각각의 위치가 특정의 염기로 되어 있는 변이체에 대한 대립형에 관한 것이지만, 이러한 대립형 뉴클레오타이드가 이중가닥의 gDNA(genomic DNA)에서 발견되는 경우, 상기한 뉴클레오타이드 서열에 대하여 상보적인 뉴클레오타이드 서열도 포함하는 것으로 해석된다. 예를 들어, 상보적인 뉴클레오타이드 서열에서 국내 돼지 내인성 레트로바이러스를 나타내는 418번째 뉴클레오타이드 “g"는 ”c"가 된다. 이러한 측면에서, 본 명세서의 모든 서열은, 특별한 언급이 없는 한, gDNA에서 센스 가닥에 있는 서열을 기준으로 한 것이다.
본 발명의 다른 양태에 따르면, 본 발명은 (a) 돼지로부터 핵산 분자를 분리하는 단계; 및 (b) 상술한 유전적 마커의 존재 유무를 확인하는 단계를 포함하는 돼지 내인성 레트로바이러스의 동정방법을 제공한다.
본 발명의 방법에 있어서, 상기 핵산 분자는 돼지의 다양한 조직으로부터 얻을 수 있으며, 바람직하게는 혈액 또는 모발, 보다 바람직하게는 모발로부터 얻을 수 있다.
본 발명의 방법에서 출발물질, 핵산 분자가, gDNA인 경우, gDNA의 분리는 당업계에 공지된 통상의 방법에 따라 실시될 수 있다(참조: Rogers & Bendich (1994)). 출발물질이 mRNA인 경우에는, 당업계에 공지된 통상의 방법에 총 RNA를 분리하여 실시된다(참조: Sambrook, J. et al., Molecular Cloning. A Laboratory Manual, 3rd ed. Cold Spring Harbor Press(2001); Tesniere, C. et al., Plant Mol. Biol. Rep., 9:242(1991); Ausubel, F.M. et al., Current Protocols in Molecular Biology, John Willey & Sons(1987); 및 Chomczynski, P. et al., Anal. Biochem. 162:156(1987)). 분리된 총 RNA는 역전사효소를 이용하여 cDNA로 합성된다. 상기 총 RNA는 동물세포로부터 분리된 것이기 때문에, mRNA의 말단에는 폴리-A 테일을 갖고 있으며, 이러한 서열 특성을 이용한 올리고 dT 프라이머 및 역전사 효소를 이용하여 cDNA을 용이하게 합성할 수 있다(참조: PNAS USA, 85:8998(1988); Libert F, et al., Science, 244:569(1989); 및 Sambrook, J. et al., Molecular Cloning. A Laboratory Manual, 3rd ed. Cold Spring Harbor Press(2001)).
본 발명의 방법에 있어서, 상기 단계 (b)는 특정 서열을 규명하는 데 이용되는 당업계에 공지된 다양한 방법을 응용하여 실시될 수 있다. 예를 들어, 본 발명에 응용될 수 있는 기술은, 형광 인 시투 혼성화(FISH), 직접적 DNA 서열결정, PFGE 분석, 서던 블롯 분석, 단일-가닥 컨퍼메이션 분석(SSCA, Orita et al., PNAS, USA 86:2776(1989)), RNase 보호 분석(Finkelstein et al., Genomics, 7:167(1990)), 닷트 블롯 분석, 변성 구배 젤 전기영동(DGGE, Wartell et al., Nucl.Acids Res., 18:2699(1990)), 뉴클레오타이드 미스매치를 인식하는 단백질 (예: E. coli의 mutS 단백질)을 이용하는 방법(Modrich, Ann. Rev. Genet., 25:229-253(1991)), 및 대립형-특이 PCR을 포함하나, 이에 한정되는 것은 아니다.
서열변화가 단일-가닥 분자내 염기 결합의 차이를 초래하여, 이동성이 다른 밴드를 출현하게 하는 데, SSCA는 이 밴드를 검출한다. DGGE 분석은 변성 구배 젤을 이용하여, 야생형 서열과 다른 이동성을 나타내는 서열을 검출한다.
다른 기술들은 일반적으로 본 발명의 유전적 마커를 포함하는 서열에 상보적인 프로브 또는 프라이머를 이용한다.
예를 들어, RNase 보호 분석에서, 본 발명의 유전적 마커를 포함하는 서열에 상보적인 리보프로브가 이용된다. 상기 리보프로브와 돼지 조직으로부터 분리한 DNA 또는 mRNA를 혼성화시키고, 이어 미스매치를 검출할 수 있는 RNase A 효소로 절단한다. 만일, 미스매치가 있어 RNase A가 인식을 한 경우에는, 보다 작은 밴드가 관찰된다.
혼성화 시그널을 이용하는 분석에서, 본 발명의 유전적 마커를 포함하는 서열에 상보적인 프로브가 이용된다. 이러한 기술에서, 프로브와 타깃 서열의 혼성화 시그널을 검출하여 직접적으로 국내 돼지 내인성 레트로바이러스의 존재 여부를 결정한다.
본 명세서에서, 용어 “프로브”는 특정 뉴클레오타이드 서열에 혼성화될 수 있는 디옥시리보뉴클레오타이드 및 리보뉴클레오타이드를 포함하는 자연 또는 변형되는 모노머 또는 결합을 갖는 선형의 올리고머를 의미한다. 바람직하게는, 프로브는 혼성화에서의 최대 효율을 위하여 단일가닥이다. 프로브는 바람직하게는 디옥시리보뉴클레오타이드이다.
본 발명에 이용되는 프로브로서, 상기 유전적 마커를 포함하는 서열에 완전하게(perfectly) 상보적인 서열이 이용될 수 있으나, 특이적 혼성화를 방해하지 않는 범위 내에서 실질적으로(substantially) 상보적인 서열이 이용될 수도 있다. 혼성화에 적합한 조건은 Joseph Sambrook, et al., Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.(2001) 및 Haymes, B. D., et al., Nucleic Acid Hybridization, A Practical Approach, IRL Press, Washington, D.C. (1985)에 개시된 사항을 참조하여 결정할 수 있다. 혼성화에 이용되는 엄격한 조건(stringent condition)은 온도, 이온세기(완충액 농도) 및 유기 용매와 같은 화합물의 존재 등을 조절하여 결정될 수 있다. 이러한 엄격한 조건은 혼성화되는 서열에 의존하여 다르게 결정될 수 있다.
본 발명의 동정방법에 따르면, 국내 돼지 내인성 레트로바이러스를 보다 정확하게 동정할 수 있으며, 결국 장기이식에 이용되는 돼지가 내인성 레트로바이러스에 의해 감염되었는지 여부를 점검할 수 있다.
PERV-A와 B는 인간 세포에 감염능을 가지고 있는 돼지 바이러스로서 여러 종류의 PERV 중에서 가장 중요하다. 따라서 이 두 가지 타입의 바이러스를 구별해 내는 방법을 구축하는 것은 매우 중요하다. 본 발명자들은 상술한 국내 돼지 내인성 레트로바이러스 연구에 기초하여, 국내 돼지 PERV-A와 B를 구별해낼 수 있는 방법을 개발하였다.
따라서 본 발명의 다른 양태에 따르면, 본 발명은 국내 돼지 PERV-A와 B를 구별하여 동정할 수 있는 프라이머를 제공한다.
본 발명의 국내 돼지 PERV-A와 B에 대한 선별 동정용 프라이머는 서열목록 제93서열 내지 제106서열에 기재되어 있다. 서열목록 제93서열 내지 제104서열에 기재된 것은 PCR 용 프라이머이고, 제105서열과 제106서열은 RFLP (Restriction fragment length polymorphism) 분석용 프라이머 세트이다. 본 발명의 PCR 용 프라이머에 있어서, 프라이머 세트는 다음 번호의 프라이머이다. 예를 들어, 제93서열은 제94서열과 프라이머 세트를 이룬다.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로서, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.
실시예
실험재료 및 실험방법
1. 공시재료 및 지놈 DNA(gDNA)의 추출
본 연구에 사용된 공시재료로는 경기도 K 종돈 농장에서 사육되고 있는 요크셔(Yorkshire)종 5두와 랜드레이스(Landrace)종 5두, 그리고 충남 C 종돈장에서 사육하고 있는 듀로크(Duroc)종 5두 그리고 버크셔(Berkshire)종 5두 총 20두이다.
선정된 공시동물에서 모발을 채취하여 4℃ 아이스박스로 실험실에 운송한 후, QIAmp Mini Kit(Qiagen, Valencia, CA)를 이용하여 지놈 DNA를 추출하였다. 추출된 지놈 DNA는 0.8% 아가로스 겔에서 전기영동으로 확인한 후, 스펙트로포토미터를 이용하여 농도를 측정하였다. 정제된 지놈 DNA는 -25℃에 보관 후 PCR을 위한 주형으로 사용되었다.
2. PERV 프로바이럴 DNA의 증폭
증폭에 이용한 프라이머 세트는 기존에 보고된 GenBank의 모든 서열의 데이터를 수집하여 모든 클래스의 env 유전자를 클로닝 하고자 하였다. 수집된 데이터를 기준으로 env 유전자의 표면 당단백질의 개시 코돈 앞과 p15E의 상동성이 놓은 지역을 이용하여 축퇴성 프라이머(degenerate primer)를 디자인하였다: 센스 프라이머, 5'-ACCTGGATCCATGCATCCCACGTTAA-3'; 안티센스 프라이머, 5'-(A/G)TCTGAAGTG GTTCTACAGAAC(A/C)G(A/G)A-3'. 이는 약 1.5 kb 정도를 증폭하게 한다. PCR 반응에는 50 ng 지놈 DNA를 사용하였다. PCR 반응에 사용된 반응 혼합물은 10 × 완충액, 1.5 mM MgCl2, dNTPs 1 mM, 프라이머는 각각 10 pM 및 Taq(Super-Bio) 2 유니트로 구성하였으며, PCR 조건은 GeneAmp PCR system 9700(Perkin-Elmer Cetus)를 이용하여 94℃ 3 분으로 핫 스타트를 1 cycle 수행 한 후, 94℃에서 30초 간 변성반응을 시킨 후, 55℃에서 30초간 어닐링반응, 그리고 72℃에서 1분간 연장 반응을 30 사이클 실시하고, 72℃에서 최종적으로 7분간 반응시켰다. 증폭된 산물은 0.8% 아가로스 겔에서 전기영동을 수행하여 확인하였다.
3. 클로닝
증폭된 약 1.5 kb의 PCR 산물은 gel extraction kit(Qiagen)를 이용하여 아가로스 겔로부터 분리/정제하였으며, 정제된 PCR 산물은 pCR2.1-TOPO 벡터 (Invitrogen)에 라이게이션 반응으로 삽입하였고, E. coli Top10F에 형질전환을 수행하여 클로닝하였다.
4. env 염기서열 분석
M13 전방향 및 M13 역방향 시퀀싱 프라이머, 증폭에 사용된 프라이머 세트를 이용하여 클로닝된 PERV env 유전자의 염기서열을 확인하였다(ABI sequencer). 염기서열 결과를 이용하여 약 1.5 kb의 염기서열을 콘티그하였으며, Genbank에 등록되어 있는 PERVs env를 참조 스트레인으로 하고 ClustalX ver. 1.81을 이용하여 얼라인먼트를 실시하였다.
5. 계통발생학적 분석
클론들의 계통분석학적 관계를 비교해 보기 위하여, 염기서열 분석이 완료된 91개 전체 클론의 염기서열을 컴퓨터 프로그램(Treecon ver. 1.3b)을 사용하여 계통분석도를 작성하였다(Van de Peer 와 De Wachter 1994). 계통 분석도는 이웃 연결(neighbor-joining), Galtie와 Gouy 그리고 Maximum parsimony 방법을 이용하여 작성하였다. 계통도의 토폴로지는 100회 리샘플링하여 부트스트랩(boostrap) 값을 구하였다.
6. PERV -A와 B의 선별 동정 방법의 개발
PERV-A와 B는 인간 세포에 감염능을 가지고 있는 돼지 바이러스로서 여러 종류의 PERV 중에서 가장 중요하다. 이 바이러스는 돼지에서 생산되지만 돼지 이외에도 인간을 포함한 여러 원숭이 종류, 햄스터, 개, 쥐, 고양이, 박쥐, 햄스터를 비롯한 여러 포유동물과 심지어 곤충 세포에 까지 감염성을 보여준다. 한편, 이 두 가지 바이러스는 인간세포에 대해서 조금 다른 감염성을 보여 준다. 아래의 표는 다양한 인간 세포에서의 PERV-A와 B의 감염능을 비교한 표로서 인간세포에서 PERV-A가 보다 감염성이 큰 것을 확인할 수 있다.
PERV-A와 B의 다양한 인간 숙주세포에서의 감염능
인간 세포 명 바이러스 역가
PERV-A PERV-B
293 12,000 <2
TE671 5,800 <2
HeLa 24,000 <2
HT1080-1 82 890
HT1080-2 16 10
FLYRD18 48 12
HOS <2 <2
U87 830 <2
MRC-5 150 <2
SupT1 <2 <2
Raji 2 <2
Molt4 2 <2
따라서 이 두 가지 타입의 바이러스를 구별해 내는 방법을 구축하는 것은 매우 중요한 문제이며 두 바이러스를 구별하는 방법을 셋업 하는 것 또한 중요한 의미를 가진다. 본 발명자들은 상술한 국내 재래 돼지의 PERVs 연구에 기초하여, 본 발명에 의해 규명되고 분류된 국내 재래 돼지의 다양한 뉴클레오타이드 서열들을 얼라인먼트하고, 이들로부터 PERV-A와 B에 특이적인 염기서열을 발견 하였다.
PERV-A와 B 각각의 특이 염기서열
염기서열 위치 염기서열
PERV-A 280-305 GTAATCCCTGGTCTCAATGACCAGGC
489-581 TCCTACCAGTTATAATCAATTTAATTATGGCCATGGGAGATGGAAAGATTGGCAACAGCGGGTACAAAAAGATGTACGAAATAAGCAAATAAG
684-720 GTACTATGGAGGCTCTGGGAGAAAGAAAGGATCTGTT
807-886 AATCCAAGAACAGAGGCCATCTCCTAACCCCTCTGATTACAATACAACCTCTGGATCAGTCCCCACTGAGCCTAACATCA
1158-1183 TAACCACACTGAAGCCTTTAATCGAA
PERV-B 278-299 TGATTAACCCCGCTGTTAAAAG
480-539 TTCCGGCCCGGGCAAGTACAAAGTGATGAAACTATATAAAGATAAGAGCTGCTCCCCATC
630-669 TTTTTATAAATATGGCGGGGGAGCAGGGTCCACTTTAACC
750-831 GGCCCTGGAGCCACCGCATAACTTGCCGGTGCCCCAATTAACCTCGCTGCGGCCTGACATAACACAGCCGCCTAGCAACGGT
842-883 TGATTCCTACCAACACGCCTAGAAACTCCCCAGGTGTTCCTG
1152-1177 CTATAGTACTGTGGTTTATGAGCAGG
I. PCR 을 이용한 방법
위에 명기된 PERV-A와 B 각각의 특이적 염기서열에 기초하여 PCR 프라이머 세트를 다음과 같이 제작하였다.
PERV-A와 B를 구별가능하게 하는 PCR 프라이머 세트
항목 프라이머 명칭 시작 위치 PCR 산물크기 PCR 프라이머 염기서열
PERV-A PERV-A1-F 281 426 bps 5'-TAATCCCTGGTCTCAATGACCA-3'
PERV-A1-R 685 5'-TTCTCCCAGAGCCTCCATAGTA-3'
PERV-A2-F 812 370 bps 5'-AAGAACAGAGGCCATCTCCTAA-3'
PERV-A2-R 1160 5'-CGATTAAAGGCTTCAGTGTGGT-3'
PERV-A3-F 697 485 bps 5'-TCTGGGAGAAAGAAAGGATCTG-3'
PERV-A3-R 1160 5'-CGATTAAAGGCTTCAGTGTGGT-3'
PERV-B PERV-B1-F 278 547 bps 5'-TGATTAACCCCGCTGTTAAAAG-3'
PERV-B1-R 824 5'-CTAGGCGGCTGTGTTATGTCAG-3'
PERV-B2-F 489 395 bps 5'-GGGCAAGTACAAAGTGATGAAA-3'
PERV-B2-R 862 5'-CAGGAACACCTGGGGAGTTTCT-3'
PERV-B3-F 648 528 bps 5'-GGGAGCAGGGTCCACTTTAAC-3'
PERV-B3-R 1152 5'-TGCTCATAAACCACAGTACTATAG-3'
위의 PERV-A에 특이적인 프라이머를 사용하면 PERV-A만 증폭되어지고 PERV-B는 증폭되지 않는다. 마찬가지로 PERV-B에 특이적인 프라이머를 사용하여 증폭하면 PERV-A는 증폭되지 않고 PERV-B만 증폭 가능하여 PERV-A와 B를 구별할 수 있다.
각각의 제작된 프라이머를 이용하여 PCR을 수행한 방법은 다음과 같다. PCR 반응에 사용된 반응 혼합물은 10 × 완충액, 1.5 mM MgCl2, dNTPs 1 mM, 프라이머는 각각 10 pM 및 Taq (Super-Bio) 2 유니트와 50 ng의 지놈 DNA로 구성하였으며, PCR 조건은 GeneAmp PCR system 9700 (Perkin-Elmer Cetus)를 이용하여 94℃ 3 분으로 핫 스타트를 1 사이클 수행 한 후, 94℃에서 30초 간 변성반응을 시킨 후, 55℃에서 30초간 어닐링반응, 그리고 72℃에서 30초간 연장 반응을 30 사이클 실시하고, 72℃에서 최종적으로 7분간 반응시켰다. 증폭된 산물은 0.8% 아가로스 겔에서 전기영동을 수행하여 확인하였다.
II. RFLP (Restriction fragment length polymorphism) 분석
다음 프라이머는 PERV-A와 B를 동시에 증폭할 수 있는 프라이머이다. 이를 이용하여 PCR 반응을 수행 한 후 제한효소 처리를 통하여 PERV-A와 B를 판별 할 수 있다.
RFLP 분석에 사용되는 프라이머
프라이머 명칭 PCR 프라이머 염기서열 비고
PERV-F 5'-ATATTCAAAAGTGGGTAAATG-3' PCR 산물 크기 PERV-A: 509 bps PERV-B: 557 bps
PERV-R 5'-GTGTTGGTGGGATGGGGGAAC-3'
위의 프라이머를 이용하여 증폭을 수행한 후 BamHI으로 제한효소 처리를 해 주면 PERV-A는 절단이 일어나서 81 bps와 420 bps로 나뉘어 지게 되고 PERV-B는 절단이 일어나지 않아 557 bps를 유지하게 된다. 따라서 증폭 반응을 수행 한 후에 BamHI으로 처리 하여 1.5% 아가로즈 겔에서 정기영동을 수행하면, PERV-A는 420 bps와 81 bps로 PERV-B는 557 bps의 밴드를 확인할 수 있다.
PCR 반응에 사용된 반응 혼합물은 10 × 완충액, 1.5 mM MgCl2, dNTPs 1 mM, 프라이머는 각각 10 pM 및 Taq (Super-Bio) 2 유니트와 50 ng의 지놈 DNA로 구성하였으며, PCR 조건은 GeneAmp PCR system 9700 (Perkin-Elmer Cetus)를 이용하여 94℃ 3 분으로 핫 스타트를 1 사이클 수행 한 후, 94℃에서 30초 간 변성반응을 시킨 후, 55℃에서 30초간 어닐링반응, 그리고 72℃에서 30초간 연장 반응을 30 사이클 실시하고, 72℃에서 최종적으로 7분간 반응시켰다. 증폭된 산물은 0.8% 아가로스 겔에서 전기영동을 수행하면 PERV-A는 509 bps의 산물과 PERV-B는 557bp의 산물을 확인 할 수 있다. 이렇게 얻어진 증폭 산물을 gel extraction kit (Qiagen)를 이용하여 아가로스 겔로부터 분리/정제하였으며, 분리 정제 된 증폭 산물을 제한 효소 처리하여 RFLP를 관찰 하였다. RFLP를 관찰하기 위하여 위의 증폭 산물은 10X 완충액, 5 유니트의 BamHI을 이용하여 반응 혼합물을 조성하였으며, 37℃에서 3시간 동안 제한효소 반응을 수행하여 1.5% 아가로즈 겔에서 정기영동을 수행하였다.
실험 결과
1. 국내 돼지의 모근으로부터 지놈 DNA 추출
돼지로부터 지놈 DNA를 분리하는 일반적인 방법은 전혈로부터 백혈구를 분리한 후 gDNA를 추출하는 방법을 이용하고 있다. 그러나 도살장이 아닌 일반 농장에서 여러 종의 돼지로부터 혈액을 채혈하는 경우 안전사고 및 기술적인 문제가 동반되기 때문에 모근을 채취하여 모근으로부터 gDNA를 추출하였다. 평균 12개의 모발에 붙어있는 모근으로부터 분리된 gDNA는 약 0.5 ㎍으로 PCR을 수행하는데 충분한 양으로 존재하였다. 이러한 방법은 이미 돼지의 PERV pol 유전자를 이용한 분포 측정에 사용된바 있으며 돼지뿐만 아니라 여러 가축으로부터 유전자 증폭용 검체를 회수하여 유전자 검사를 요하는 시험에 적절히 이용되어질 수 있으리라 판단된다.
PERV env 유전자 클로닝 및 분포 조사
돼지로부터 분리한 지놈 상에 존재하는 PERV env 유전자를 클로닝하기 위해, 클래스 A, B 및 C에 공통적인 프라이머를 이용하여 PCR을 수행하였다. PCR 증폭 반응을 통하여 PERV env 유전자의 개시 코돈을 포함하여 p15E의 일부를 포함하는 약 1.5 kb의 산물을 얻을 수 있었다.
얻어진 산물을 이용하여 pCR2.1-TOPO 벡터에 클로닝하여 버크셔 17개, 듀로크 22개, 랜드레이스 21개 및 요크셔 31개로 총 91개의 클론을 확보하였다. 확보된 클론은 M13 전방향 및 M13 역방향 시퀀싱 프라이머와 클로닝에 사용한 프라이머를 이용하여 4회 이상 염기서열 분석을 수행하여 산물의 염기서열을 결정하였다.
GenBank에 등록된 바이러스 주를 기준으로 염기 서열을 얼라인먼트한 결과 계통 분석학적 계통도를 그리기 전에 크게 클래스 A 및 클래스 B로 나누어짐을 알 수 있었다. 버크셔로부터 분리된 클론 17개 중 11개는 클래스 A에 6개는 클래스 B에 속하였으며, 듀로크로부터 분리된 클론 22개 중 10개는 클래스 A 그리고 11개는 클래스 B로, 랜드레이스로부터의 클론 21개 중 13개가 클래스 A에 8개가 클래스 B, 요크셔의 31개 클론 중 18개가 클래스 A, 13개가 클래스 B로 분류 되었다. 클래스 간의 분포를 보면 클래스 A가 57%, 클래스 B가 43%로 두 클래스 사이의 분포는 큰 차이를 보이지 않았으며, 개체내의 분포도 버크셔 클래스 A와 클래스 B는 각각 64%와 36%, 듀로크 45%와 55%, 랜드레이스 61%와 39%, 요크셔 58%와 42%의 분포를 보였다(표 5).
한국내 돼지에서 얻은 PERV env 클론
항목 돼지 종류
버크셔 듀로크 랜드레이스 요크셔
클래스 PERV-A PERV-B PERV-A PERV-B PERV-A PERV-B PERV-A PERV-B
클론 수 11(4) 6(2) 10(3) 11(4) 13(7) 8(2) 18(6) 13(2)
상기 표 1에서 괄호 내의 숫자는 올바른 오픈 리딩 프레임을 갖는 클론의 수를 나타낸다.
PERVs는 종에 따라 10에서 50개 프로바이러스 유전자가 돼지 지놈에 존재하며, env 유전자의 염기서열 특히 당단백질의 변이부위 A(variable region A: VRA)의 차이에 의해 최소한 3 종류의 클래스(A, B 및 C)의 PERVs가 감염성 바이러스로 존재하는 것으로 알려져 있다(Akiyoshi 등, 1998). 지놈 상에 존재하는 PERV 프로바이러스는 대부분이 결실, 삽입 혹은 점 돌연변이 등에 의하여 대부분 올바른 바이러스 입자를 생성하지 못하지만, 클론 중 버크셔에서 클래스 A 4개와 클래스 B 2개, 듀로크에서 클래스 A와 B 각각 3개와 4개, 랜드레이스 5개와 2개, 요크셔 6개와 2는 올바른 ORF을 갖는 바이러스가 생산 될 수 있는 클론임을 확인하였다. 이는 전체 조사된 클론 중에 30%라는 상당히 높은 수치로서 이종간 장기 이식 시 바이러스가 생성될 수 있어 절대 간과해서는 안 될 중요한 인수 공통 감염 요인임을 알 수 있었다.
프라이머의 제작에 있어 클래스 A, B 및 C 모두에 대한 공통 프라이머를 이용하여 프로바이러스 DNA를 증폭하였지만 클래스 C는 나오지 않았다. 클래스 C는 non-humantropic으로 조직과 세포의 종류에 따라 발현량이 다르며, 그 발현량 역시 현저히 낮은 것으로 보고 되어 있다(Stefan 등, 2003; Steffi 등, 2000). 하지만, 최근 연구에 의해서 클래스 A와 C의 재조합체가 인간세포 감염성에 큰 영향을 미친다는 보고와 함께 중요한 클래스로 부각되고 있다(Wood 등, 2004). 본 실험의 공시재료로 사용된 돼지의 증폭산물에서 클래스 C가 발견되지 않은 이유는 프라이머의 부적절성이라기 보다는 클론들의 N 값이 작아서 클래스 C가 나오지 않은 것이라 생각되며 더 많은 클론의 분석이 요구된다.
PERV env 유전자 염기 서열 분석 및 아미노산 서열 분석
분석된 염기 서열을 토대로 아미노산 서열과 염기 서열을 분석한 결과, 대부분의 클론들이 결실, 삽입 및 점 돌연변이 등에 의해 올바른 바이러스를 생성하지 못하는 비기능성 클론들이었으나, 클론 중 클래스 A로 분류된 18개 클론과 클래스 B로 분류된 10개, 총 28개의 클론은 올바른 오픈 리딩 프레임(ORF)을 가지고 있었다.
도 4의 표는 ORF가 맞는 클론과 대표적인 기준 주간 intra/inter-클래스 간 유전자와 아미노산 서열간 상동성 비교를 한 것이다. 표에서 위쪽 및 아래쪽 삼각형은 각각 뉴클레오타이드 서열 상동성 및 아미노산 서열 상동성을 나타낸다. Y12238은 PERV-A에 대한 기준 바이러스주로 이용하였고, Y12239는 PERV-B에 대한 기준 바이러스주로 이용하였다. 상동성 계산은 1539개의 뉴클레오타이드 서열 및 513개의 아미노산 서열에 기초하여 계산하였다.
돼지 개체 간 차이를 제외한 클래스 내의 염기 서열 상동성은 95.9%에서 99.9%로 높은 상동성을 확인할 수 있었으며, 클래스 A와 B에서는 64.3%에서 66.6%로 많은 차이를 지님을 알 수 있었고, 아미노산 서열간의 상동성도 염기 서열 상동성과 비슷한 차이를 확인할 수 있었다.
PERV env 유전자는 발현되면 세포막으로 이동되어 바이러스의 코어 부분을 세포막과 함께 둘러쌈으로서 바이러스를 생성하게 된다. 이 엘벨로프 단백질은 막투과 도메인 내에 절단 위치(Arg/Lys-X-Lys-Arg)가 존재하여, 숙주 세포의 수용체에 결합하게 되는 표면 당단백질로 HIV-1의 경우 gp120에 해당하는 gp70부분과 인지질 이중막 사이에 묻혀 있게 되는 막투과 도메인(p15E)으로 나뉘게 된다. gp70은 8개의 잠재적 당화 위치를 가지고 있는 것으로 알려져 있으며 감염성에 큰 영향을 주는 것으로 보고 되어 있다(Kenji 등, 2003; Oldmixon 등, 2002). http://swissmodel.expasy.org//SWISS-MODEL.html 웹 사이트의 단백질 구조 예측 서비스를 통하여 PERV의 엘벨로프 단백질은 당단백질로 클래스 A에서 모두 8개의 잠재적인 당화 위치(Asn-X-Thr/Ser)를 발견할 수 있었으며, 클래스 B 에서는 5개의 당화 위치를 확인할 수 있었다(참조: 도 1 및 도 2).
gp70과 p15E로 분리하는 절단 위치는 Arg/Lys-X-Lys-Arg으로(Cianciolo 등, 1984), 459에서 462까지의 아미노산 서열에 위치하고 있었으며, 아미노산 서열 465에서 490까지의 부분이 막투과 도메인으로 나선 구조를 가지고 소수성 아미노산인 Leu, Ala 및 Val이 많이 분포하며, 클래스 A와 B 모두 잘 보전 되어 있음을 알 수 있었다.
특이할 만 한 사항으로는 분석한 클론들 중 랜드레이스로부터 얻은 L9 클론은 절단 위치의 아미노산 잔기가 Arg/Lys-X-Lys-Arg에서 Arg/Lys 잔기 대신에 트립토판으로 대체 되어 있음을 알 수 있었다. 이 클론은 올바른 절단이 일어나지 못하여 올바른 엔밸로프의 기능을 수행하지 못하리라 추측된다. 당화 위치 Asn-X-Thr/Ser에서, L9 클론의 318번째 잔기는 Thr/Ser 대신 Lys으로 바뀌어 있으며, Duroc으로부터 얻은 D8 클론 역시 388 번째 아미노산 잔기가 Asn 잔기 대신에 Ser으로 바뀌어져 있어서 N-당화가 되지 않을 것으로 예측된다. N-당화가 만약 올바로 이루어지지 않으면 감염능 등의 이상 등 변화가 예상된다.
PERV env 유전자 염기서열을 이용한 계통 분석
염기 서열 분석된 총 91개 클론의 염기서열과 GenBank에 등록된 PERV-A(AY312521, AF507940, Y12238), PERV-B(AJ288587, AJ288589, Y12239), PERV-C(AF038600, AF402662) 그리고 outgroup으로 PERV-E(AF356698)를 기준 바이러스주로 사용하여 Neighbor-Joining 방법을 이용하여 계통 분석학적 관계를 알아보았다. PERV-E는 유전학적으로 사람에 존재하는 인간 내인성 레트로바이러스와 유사하며 양적으로 PERV-A, B 및 C와 비슷하게 존재하나 유전적 거리가 멀어 이를 outgroup으로 선정 하였다. 도 3은 전체 91개 바이러스 주를 포함한 Tree를 만든 후 유사한 것을 제외하여 나타낸 것이다. 클래스 B는 100%의 bootstrap 값으로 클레이드(clade)를 이루는 반면 클래스 A와 C는 95%의 값으로 같은 클레이드를 이루며, 클래스 C만이 100%로 나뉘어 졌다. 클래스 C는 지금까지 2 바이러스주(AF038600, Af402662)만이 GenBank에 등록되어 있기에 제한적으로 분석될 수밖에 없었다.
클래스 A와 B의 염기서열이 약 35% 이상 다르기 때문에(참조: 도 4), 실제 계통분석 전에 이 두 서브그룹 간 바이러스 주 구분을 할 수 있었으며, 계통분석을 통한 서브그룹 분석은 표 1의 결과와 동일하게 나왔으며 C 형은 나오지 않았다.
PERV -A와 B의 선별 동정
상술한 프라이머를 이용하여 PCR을 실시한 결과, PERV-A 또는 PERV-B에 특이적인 기대되는 크기의 증폭 산물이 형성되었다. 따라서, 본 발명자들에 의해 개발된 프라이머들은 PERV-A 또는 B에 특이적인 것으로서, 이를 이용하면 PERV-A 및 PERV-B를 특이적으로 동정할 수 있음을 알 수 있다(참조: 도 5a).
RFLP 분석을 실시한 결과, BamHI으로 증폭산물을 제한효소 처리를 해 주면 PERV-A는 절단이 일어나서 81 bps와 420 bps로 나뉘어 지게 되고, PERV-B는 절단이 일어나지 않아 557 bps를 유지하게 된다. 따라서 증폭 반응을 수행 한 후에 BamHI으로 처리 하여 1.5% 아가로즈 젤에서 전기영동을 수행하면, PERV-A는 420 bps와 81 bps로 PERV-B는 557 bps의 밴드를 확인할 수 있었다(참조: 도 5b).
상술한 바와 같이, 본 발명의 env 단백질 및 유전자는 무균 돼지 내인성 레트로바이러스에 대하여 특이적으로 발현되는 것이며, env 유전자 내의 유전적 마커를 이용하는 경우에는 무균 돼지 내인성 레트로바이러스를 정확하게 동정할 수 있다.
이상으로 본 발명의 특정한 부분을 상세히 기술하였는 바, 당업계의 통상의 지식을 가진 자에게 있어서 이러한 구체적인 기술은 단지 바람직한 구현예일 뿐이며, 이에 본 발명의 범위가 제한되는 것이 아닌 점은 명백하다. 따라서, 본 발명의 실질적인 범위는 첨부된 청구항과 그의 등가물에 의하여 정의된다고 할 것이다.
참조 문헌
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cttaaaaata agtttcactg aaaaaggaaa acaagaaaat attcaaaagt gggtaaatgg 660 tatgtcttgg ggaatagtgt actatggagg ctctgggaga aggaaaggat ctattctaac 720 tattcgcctc agaatagaaa ctcagatgga acctccggtt gctataggac caaataagtg 780 tttggccgaa caaggacctc caatccaaga acagaggcca tctcttaacc cctctgatta 840 caatacagcc tctggatcag tccccactga gcctaacatc actattaaaa caggggcaaa 900 acttttagcc tcatccaggg agcttttcaa gctcttaact ccacaactcc agaggctacc 960 tcttcttgtt ggctttgctt agcttcaggc ccaccttact atgaggaacg gctagaggag 1020 ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc caaaataagc 1080 ttacccttac tgaggtttct ggaaaaggca cctgcatagg gagggttccc ccatcccacc 1140 aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag tatctggtac 1200 ccggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt tccaccctgg 1260 ttttcaacca aactaaagac ttttgcgtta tggtccaaat tgtcccccgg gtgtactact 1320 atcccgaaaa agcggtcctt gatgaatatg tctatagata taatcagcca aaaagagaac 1380 ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc gtgggaacag 1440 gaacggctgc cctaatcaca ggaccacaac agctggagaa aggacttagt gacctacatc 1500 gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaaccta gaggaatccc 1560 taacctcctt gtctgaagtg gttctacaga acagga 1596 <210> 2 <211> 1605 <212> DNA <213> porcine endogenous retrovirus <400> 2 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgca tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actctggtac aggtattaat attaacagca ctcaagggga ggctccctta 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg cctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt atgtttgccc aggacccccc 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaagtggg 660 taaatggtat gtcttgggga atagtgtact atggaggctc tgggagaaag aaaggatctg 720 ttctgactat tcgcctcaga atagaaactc agatggaacc tccggttgct ataggaccaa 780 ataagggttt ggccgaacaa ggacctccaa tccaagaaca gaggccatct cctaacccct 840 ctgattacaa tacaacctct ggatcagtcc ccactgagcc taacatcact attaaaacag 900 gggcgaaact ttttagcctc atccagggag cttttcaagc ccttaactcc acgactccag 960 aggctacctc ttcttgttgg ctttgcttag cttcgggccc accttactat gagggaatgg 1020 ctagaggagg gaaattcaat gtgacaaagg aacatagaga ccaatgtaca tggggatccc 1080 aaaataagct tacccttact gaggtttctg gaaaaggcac ctgcataggg agggttcccc 1140 catcccacca acacctttgt aaccacactg aagcctttaa tcgaacctct gagagtcagt 1200 atctggtacc tggttatgac aggtggtggg catgtaatac tggattaacc ccttgtgttt 1260 ccaccttggt tttcaaccaa actaaagact tttgcgttat ggtccaaatt gtcccccggg 1320 tgtactacta tcccgaaaaa gcagtccttg atgaatatga ctatagatat aatcggccaa 1380 aaagagagcc catatccctg acactagctg taatgctcgg attgggagtg gctgcaggcg 1440 tgggaacagg aacggctgcc ctaatcacag gaccacaaca gctggagaaa ggacttagta 1500 acctacatcg aattgtaacg gaagatctcc aagccctaga aaaatctgtc agtaacctgg 1560 aggaatccct aacctcctta tctgaagtgg ttctacagaa cagga 1605 <210> 3 <211> 1606 <212> DNA <213> poricine endogenous retrovirus <400> 3 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctcccttg 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt acgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcata 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tatcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaata 900 ggggcgaaac tttttagcct catccaggga gcttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaaggca cctgcatagg gatggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcaa 1200 tatctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccttgg ttttcaacca aactaaagac ttttgcgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagagc ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc 1440 gtgggaacag gaacggctgc cctaatcaca ggaccgcaac agctggagaa aggacttagt 1500 aacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaacctg 1560 gaggaatccc taacctcctt gtctgaagtg gttctacaga acagaa 1606 <210> 4 <211> 1605 <212> DNA <213> porcine endogenous retrovirus <400> 4 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctcccttg 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt acgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aataaaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaat ttttagcctc atccagggag cttttcaagc tcttaactcc acgactccag 960 aggctacctc ttcttgttgg ctttgcttag cttcgggccc accttactat gagggaatgg 1020 ctagaggagg gaaattcaat gtgacaaagg aacatagaga ccaatgtaca tggggatccc 1080 aaaataagct tacccttact gaggtttctg gaaaaggcac ctgcataggg atggttcccc 1140 catcccacca acacctttgt aaccacactg aagcctttaa tcgaacctct gagagtcaat 1200 atctggtacc tggttatgac aggtggtggg catgtaatac tggattaacc ccttgtgttt 1260 ccaccttggt tttcaaccaa actaaagact tttgcgttat ggtccaaatt gtcccccggg 1320 tgtactacta tcccgaaaaa gcagtccttg atgaatatga ctatagatat aatcggccaa 1380 aaagagagcc catatccctg acactagctg taatgctcgg attgggagtg gctgcaggcg 1440 tgggaacagg aacggctgcc ctaatcacag gaccgcaaca gctggagaaa ggacttagta 1500 acctacatcg aattgtaacg gaagatctcc aagccctaga aaaatctgtc agtaacctgg 1560 aggaatccct aacctcctta tctgaagtgg ttctacagaa cagga 1605 <210> 5 <211> 1594 <212> DNA <213> porcine endogenous retrovirus <400> 5 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggagagcc gaaaagactg 60 aaaaccccct taagcttcgc ctccatcgct tggttcctta ctctgtaaat aactcctcaa 120 gttaatggta aacgccacag aactcccaca aacccttatc tctcacctgg ttacttactg 180 actccagtac aggtattact attaacagca ctcaagggga ggctccctta gggacctggt 240 ggcctgagtt atatgtctgc cttcgatcgg taatccctgg tctcaacgac caggccacac 300 cccccgatgt actccgtgct tatgggtttt atgtttgccc aggaccccca aataatgaag 360 aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta acttctaatg 420 atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct tttgttaaca 480 atcctaccag ttataatcaa tttaattacc gccatgggaa atggaaagat tggcaacagc 540 aggtacaaaa agatgtacga aataagcaaa taagctgtaa tttgttagac ctagattact 600 taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg gtaaatggta 660 tgtcttgggg aatagtgtac tatggaggct ctgggagaag gaaaggatct attctaacta 720 ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca aataagtgtt 780 tggccgaaca aggacctcca atccaagaac agaggccatc tcttaacccc tctgattaca 840 atacagcctc tggatcagtc cccactgagc ctaacatcac tattaaaaca ggggcaaaac 900 ttttagcctc atccagggag cttttcaagc tcttaactcc acaactccag aggctacctc 960 ttcttgttgg ctttgcttag cttcaggccc accttactat gaggaacggc tagaggaggg 1020 aaattcaatg tgacaaagga acatagagac caatgtacat ggggatccca aaataagctt 1080 acccttactg aggtttctgg aaaaggcacc tgcataggga gggttccccc atcccaccaa 1140 cacctttgta accacactga agcctttaat cgaacctctg agagtcagta tctggtaccc 1200 gattatgaca ggtggtgggc atgtaatact ggattaaccc cttgtgtttc caccctggtt 1260 ttcaaccaaa ctaaagactt ttgcgttatg gtccaaattg tcccccgggt gtactactat 1320 cccgaaaaag cggtccttga tgaatatgac tatagatata atcagccaaa aagagaaccc 1380 atatccctga cactagctgt aatgctcgga ttgggagtgg ctgcaggcgt gggaacagga 1440 acggctgccc taatcacagg accacaacag ctggagaaag gacttagtga cctacatcga 1500 attgtaacgg aagatctcca agccctagaa aaatctgtca gtaacctaga ggaatcccta 1560 acctccttgt ctgaagtggt tctacagaac agga 1594 <210> 6 <211> 1707 <212> DNA <213> porcine endogenous retrovirus <400> 6 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccccc aaataatgaa gaatattgtg 480 gaaatcctca ggatttcttt tgcaagcaat ggagctgcgt aacttctaat gatgggaatt 540 ggaaatggcc agtctctcag caagacagag taagttactc ttttgttaac aatcctacca 600 gttataatca atttaattat ggccatggga gatggaaaga ttggcaacag cgggtacaaa 660 aagatgtacg aaataagcaa ataagctgtc attcgttaga cctagattac ttaaaaataa 720 gtttcactga aaaaggaaaa caagaaaata ttcaaaagtg ggtaaatggt atgtcttggg 780 gaatagtgta ctatggaggc tctgggagaa agaaaggatc tgttctgact attcgcctca 840 gaataaaaac tcagatggaa cctccggttg ctataggacc aaataagggt ttggccgaac 900 aaggaccccc aatccaagaa cagaggccat ctcctaaccc ctctgattac aatacaacct 960 ctggatcagt ccccactgag cctaacatca ctattaaaac aggggcgaaa ctttttaacc 1020 tcatccaggg agcttttcaa gctcttaact ccacgactcc agaggctacc tcttcttgtt 1080 ggctttgctt agcttcgggc ccaccttact atgagggaat ggctagagga gggaaattca 1140 atgtgacaaa ggaacataga gaccaatgta catggggatc ccaaaataag cttaccctta 1200 ctgaggtttc tggaaaaggc acctgcatag ggatggttcc cccatcccac caacaccttt 1260 gtaaccacac tgaagccttt aatcgaacct ctgagagtca gtatctggta cctggttatg 1320 acaggtggtg ggcatgtaat actggattaa ccccttgtgt ttccaccttg gttttcaacc 1380 aaactaaaga cttttgcgtt atggtccaaa ttgtcccccg ggtgtactac tatcccgaaa 1440 aagcagtcct tgatgaatat gactatagat ataatcggcc aaaaagagag cccatatccc 1500 tgacactagc tgtaatgctc ggattgggag tggctgcagg cgtgggaaca ggaacggctg 1560 ccctaatcac aggaccgcaa cagctggaga aaggacttag taacctacat cgaattgtaa 1620 cggaagatct ccaagcccta gaaaaatctg tcagtaacct ggaggaatcc ctaacctcct 1680 tgtctgaagt ggttctacag aacagaa 1707 <210> 7 <211> 1597 <212> DNA <213> porcine endogeous retrovirus <400> 7 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacagaac cccctctggc agtgggaccc 720 gataaagtac tggctgaaca ggggcccccg gccctggagc caccgcataa cttgccggtg 780 ccccaattaa cctcgctgcg gcctgacata acacagccgc ctagcaacgg taccactgga 840 ttgattccta ccaacacgcc tagaaactcc ccaggtgttc ctgttaagac aggacagaga 900 ctcttcagtc tcatccaggg agctttccaa gccatcaact ccactgaccc tgatgccact 960 tcttcttgtt ggctttgtct atcctcaggg cctccttatt atgaggggat ggctaaagaa 1020 ggaaaattca atgtgaccaa agagcataga aatcaatgta catgggggtc ccgaaataag 1080 cttaccctca ctgaagtttc cgggaagggg acatgcatag gaaaagctcc cccatcccac 1140 caacaccttt gctatagtac tgtggtttat gagcaggcct cagaaaatca gtatttagta 1200 cctggttata acaggtggtg ggcatgcaat actgggttaa ccccctgtgt ttccacctca 1260 gtcttcaacc aatccaaaga tttctgtgtc atggtccaaa tcgtcccccg agtgtactac 1320 catcctgagg aagtggtcct tgatgaatat gactatcggt ataaccgacc aaaaagagaa 1380 cccgtatccc ttaccctagc tgtaatgctc ggattaggga cggccgttgg cgtaggaaca 1440 gggacagctg ccctgatcac aggaccacag cagctagaga aaggacttgg tgagctacat 1500 gcggccatga cagaagatct ccgagcctta gaggagtctg ttagcaacct agaagagtcc 1560 ctgacttctt tgtctgaagt ggttctacag aacagga 1597 <210> 8 <211> 1592 <212> DNA <213> porcine endogenous retrovirus <400> 8 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtagaaagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacagt gactccccag 120 gccagtagta aacgccttat agacagctcg gacccccata gacccttatc ccttacgtgg 180 ctgattattg accctgatac gggcgtcact gttaatagca ctcgaggtgt cgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 gaatactgtg ggggttccag ggaatccttc tgtgggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatggaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggaggggg agcagggtcc 660 actttaacta ttcgccttag gatagagacg gggacggaac cccctgtggc agtgggaccc 720 aacatagtac tggctgaaca ggggcccccg gccctggagc caccacacaa cttgccggtg 780 ccccagttaa cctcgctgcg gcctgacata acacagccgc ctgacacagt accactggat 840 tgatttccta ccaacacgcc tagaaactcc ccggtgttcc tgttaagaca ggacagagac 900 tcttcagtct catccaggga gctttccagc catcaactcc accgaccccg atgccagttc 960 ttcttgttgg ctttgtctat cctcaggcct ccttattatg agggaatggc tagagaagga 1020 attcattgtg accaaagagc atagaaatca attgtacatg ggggtcccga aataagctca 1080 ccctcactga agtttctggg aaggggacat gcataggaaa agctccccca tcccaccaac 1140 aacctttgca atagtactat ggtttatgag caggccttag aaaatcagta tttagtacct 1200 ggttataact gatggtgggc atgcaatact gggttaactc cctgtgtttc taccacagtc 1260 tttaaccaat ccaaagattt ctgtgtcatg gtccagctcg tcccctgggt acactaccat 1320 cctgaggaag tagtcatcga tgagtatgac tatcggccta cccgctcaaa aagagagccc 1380 gtgaccctta accctagccg ttatactagg attagggatg gctggtgtgg ggacagggac 1440 agctgccctg atcacaggac cacagcagct agaaaaagga cttggtgagc tacatgcggc 1500 cataacagaa gatctccgag ccttagagga atctgttaac aacctagaag aatccctgat 1560 ttctttatct gaagtggttc tacagaaccg aa 1592 <210> 9 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 9 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggccccctta 240 gggacctggt ggcctgagtt atatgtctgc cttcgatcag taatccctgg cctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt atgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtaaga aataagcaaa ttagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataaggggt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaac tttttagcct catccaggga gcttttcaag cccttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcctcgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaaggca cctgcatagg gagggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag 1200 tatctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccttgg ttttcaacca aactaaagac ttttgcgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcggtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagagc ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcagac 1440 gtgggaacag gaacggctgc cctaatcaca ggaccacaac agctagagaa aggacttagt 1500 aacctacatc gaattgtaac ggaagatctc caagcccgag aaaaatctgt cagtaacctg 1560 gaggaatccc taacctcctt atctgaagtg gttctacaga accgaa 1606 <210> 10 <211> 1605 <212> DNA <213> porcine endogenous retrovirus <400> 10 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctcccttg 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg cctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt atgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaac tttttagcct catccaggga gcttttcaag cccttaactc cacgactcca 960 gaggctacct cttcttgtgg ctttgcttag cttcgggccc accttactat gagggaatgg 1020 ctagaggagg gaaattcaat gtgacaaagg aacatagaga ccaatgtaca tggggatccc 1080 aaaataagct tacccttact gaggtttctg gaaaaggcac ctacataggg atggttcccc 1140 catcccacca acacctttgt aaccacactg aagcctttaa tcgaacctct gagagtcagt 1200 atctggtacc tggttatgac aggtggtggg catggagtac tggattaacc ccttgtgttt 1260 ccaccttggt tttcaaccaa actaaagact tttgcgttat ggtccaaatt gtcccccggg 1320 tgtactacta tcccgaaaaa gcagtccttg atgaatatga ctatagatat aatcggccaa 1380 aaagagagcc catatccctg acactagctg taatgctcgg attgggagtg gctacaggcg 1440 tgggaacagg aacggctgcc ctaatcacag gaccgcaaca gctggagaaa ggacttagta 1500 acctacatcg aattgtaacg gaagatctcc aagccctaga aaaatctgtc agtaacctgg 1560 aggaatccct aacctcctta tctgaagtgg ttctacagaa cagga 1605 <210> 11 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 11 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct tatgcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctcccttg 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt acgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagactact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aataaaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaac tttttaacct catccaggga gcttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaaggca cctgcatagg gatggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag 1200 tatctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccttgg ttttcaacca aactaaagac ttttgcgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagagc ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc 1440 gtgggaacag gaacggctgc cctaatcaca ggaccgcaac agctggagaa aggacttagt 1500 aacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaacctg 1560 gaggaatccc taacctcctt atctgaagtg gttctacaga accgaa 1606 <210> 12 <211> 1597 <212> DNA <213> porcine endogenous retrovirus <400> 12 atgcatccca cgttaagctg gagccacgtc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccctgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtc ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacggaac cccctgtggc agtgggaccc 720 gataaagtac tggctgaaca ggggcccccg gccctggagc caccgcataa cttgccggtg 780 ccccaattaa cctcgctgcg gcctgacata acacagccac ctagcaacgg taccactgga 840 ttgattccta ccaacacgcc tagaaactcc ccaggtgttc ctgttaagac aggacagaga 900 cccttcagtc tcatccaggg agctttccaa gccatcaact ccaccgaccc cgatgccact 960 tcttcttgtt ggctttgtct atcctcaggg cctccttatt atgaggggat ggctaaaaaa 1020 ggaaaattca atgtgaccaa agagcataga aatcaatgta catgggggtc ccgaaataag 1080 tttaccctca ctgaagtttc cgggaagggg acatgcatag gaaaagctcc cccatcccac 1140 caacaccttt gctatagtac tgtggtttat gagcaggcct cagaaaatca gtatttagta 1200 cctggttata acaggtggtg ggcatgcaat actgggttaa ccccctgtgt ttccacctca 1260 gtcttcaacc aatccaaaga tttctgtgtc atggtccaaa tcgtcccccg agtgtactac 1320 catcctgagg aagtggtcct tgatgaatat gactatcggt ataaccgacc aaaaagagaa 1380 cccgtatccc ttaccctagc tgtaatgctc ggattaggga cggccgttgg cgtaggaaca 1440 gggacagctg ccctgatcac aggaccacag cagctagaga aaggacttgk tgarctacat 1500 gcggccatga cagaagatct ccgagcctta gaggagtctg ttagcaacct agaagagtcc 1560 ctgacttctt tgtctgaagt ggttctacag aaccgga 1597 <210> 13 <211> 1592 <212> DNA <213> porcine endogenous retrovirus <400> 13 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agcgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg tatgagctgg ggaatagttt tttataaata tggcggggga gcagggtcca 660 ctttaaccat tcgccttagg atagagacgg ggacagaacc ccctgtggca gtgggacccg 720 ataaaatact ggctgaacag gggcccccgg ccctggagcc accgcataac ttgccggtgc 780 cccaattaac ctcgctgcgg cctgacataa cacagccgcc tggcaacggt accactggat 840 tgattcctac caacatgcct agaaactccc ccggtgttcc tgttaagaca ggacagagac 900 tctttagtct catccaggga gtttccaagc catcaactcc accaactccg atgccacttc 960 ttcttgttgg ctttgtctat cctcagggct tccttattat gagagaatgg ctagagaaag 1020 gaaattcaat gtgaccaaag agcatagaaa tcaatgtaca tgggggtccc gaaataagct 1080 taccttcact gaagtttccg ggaaggggac atgcatagga aaagctcccc catcccacca 1140 acacctttgc aatagtacta tggtttatga gcaggcctca gaaaatcagt atttagtacc 1200 tggttataac aggtggtggg catgcaatac tgggttaact ccctgtgttt ctaccacagt 1260 ctttaaccaa tccaaagatt tctgtgtcat ggtccagctc gtcccctggg tatactacca 1320 tcctgaggaa gtagtcattg atgagtatga ctatcggcct acccgctcaa aaagagagcc 1380 cgtgaccctt accctagccg ttatacttgg attagggatg gccggtgtgg ggacagggac 1440 agctgccctg atcacaggac cacagcagct agaaaaagga cttggtgagc tacatgcagc 1500 catgacagaa gatctccgag ccttagagga atctgttagc aacctagaag agtccctgac 1560 ttctttgtct gaagtggttc tacagaacag ga 1592 <210> 14 <211> 1597 <212> DNA <213> porcine endogenous retrovirus <400> 14 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggagagcc gaaaagactg 60 aaaaccccct taagcttcgc ctccatcgct tggtccttag ctctgtaaat aactcctcaa 120 gttaatggta aacgcctgaa gctgcccaca aacccttaac tctcacctgg ttacttactg 180 actccagtac aggtattact attaacagca ctcaagggga ggctccctta gggacctggt 240 ggcctgagtt atatgtctgc cttcgatcgg taatccctgg tctcaacgac caggccacac 300 cccctgatgt actctgtgct tacaggttta atgtttgccc aggaccccca aataatgaag 360 aatattgtgg aaatcctcag gatttctttt gcaagcaagg gagctgcgta acttctaatg 420 atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct tttgttaaca 480 gtcctaccag ttataatcaa tttaattatg gccatggggg atggaaagat tggcaacagc 540 gggtacaaaa agatgtacga aataagcaaa taagctgtaa ttcgttagat ctagattact 600 taaaaataag ttttactgaa aaaggaaaac aagaaaatat tcaaaagtgg gtaaatggta 660 tgtcttgggg aataatgtac tatggaggca ctgggagaag gaaaggatct gttctgacta 720 ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca aataagggtt 780 tgggctgaac aaggacctcc aatccaagaa cagaggccat ctcctaaccc ctctgtttac 840 aatacaacct ctggattagt cccccctgag cctaacttca ctattaaaac aggggcgaaa 900 ctttttagcc tcatccaggg agcttttcaa gctcttaact ccacgactcc agaggctacc 960 tcttcttgtt ggctttgctt agctttgagc ccaccttact atgagggaat ggctagagga 1020 gggaaattca atgtgacaaa ggaacataga gaccaatgta catggggatc ccaaaataag 1080 cttaccctta ctgaggtttc tggaaaaggc acctgcatag ggagggttcc cccatcccac 1140 caacaccttt gtaaccacac tgaagccttt aatcgaacct ctgagagtca gtacctggta 1200 cctggttatg acaggtggtg ggcatgtaat actggattaa ccccttgtgt ttccaccctg 1260 gttttcaacc aaactaaaga cttttgtgtt atggtccaaa ttgtcccccg ggtgtactac 1320 tatcccgaaa aagcggtcct tgatgaatat gactatagat ataatcggcc aaaaagagaa 1380 cccatatccc tgacactagc tgtaatgctc ggattgggag tggctgcagg cgtgggaaca 1440 ggaacggctg ccctaatcac aggaccacaa cagctggaga aaggacttag tgacctacat 1500 cgaattgtaa cggaagatct ccaagcccta gaaaaatctg tcagtaacct agaggaatcc 1560 ctatcctcct tgtctgaagt ggttctacag aacagaa 1597 <210> 15 <211> 1598 <212> DNA <213> porcine endogenous retrovirus <400> 15 atgcatccca cgttaagctg gcgcaacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacagaac cccctctggc agtgggaccc 720 gataaagtac tggctgaaca ggggcccccg gccctggagc caccgcataa cttgccggtg 780 ccccaattaa ccttgctgcg gcctgacata acacagccgc ctagcaacgg taccactgga 840 ttgattccta ccaacacgcc tagaaactcc ccaggtgttc ctgttaagac aggacagaga 900 ctcttcagtc tcatccaggg agctttccaa gccatcaact ccactgaccc tgatgccact 960 tcttcttgtt ggctttgtct atcctcaggg ccctccttat tatgagggga tggctaaaga 1020 aggaaaattc aaatgtgacc aagagcatag aaatcaatgt acatgggggt cccgaaataa 1080 gcttaccctc actgaagttt ccgggaaggg gacatgcata ggaaaagctc ccccatccca 1140 ccaacacctt tgctatagta ctgtggttta tgagcaggcc tcagaaaatc agtatttagt 1200 acctggttat aacaggtggt gggcatgcaa tactgggtta cccccctgtg tttccacctc 1260 agtcttcaac caatccaaag atttctgtgt catggtccaa atcgtccccc gagtgtacta 1320 ccatcctgag gaagtggtcc ttgatgaata tgactatcgg tataaccgac caaaaagaga 1380 acccgtatcc cttaccctag ctgtaatgct cggattaggg acggccgttg gcgtaggaac 1440 agggacagct gccctgatca caggaccaca gcagctagag aaaggacttg gtgagctaca 1500 tgcggccatg acagaagatc tccgagcctt agaggagtct gttagcaacc tagaagagtc 1560 cctgacttct ttgtctgaag tggttctaca gaacagga 1598 <210> 16 <211> 1596 <212> DNA <213> porcine endogenous retrovirus <400> 16 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacagt gactccccag 120 gccagtagta aacgccttat agacagctcg gacccccata gacccttatc ccttacgtgg 180 ctgattattg accctgatac gggcgtcact gttaatagca ctcgaggtgt cgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 gaatactgtg ggggttccag ggaatccttc tgtgggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatggaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggaggggg agcagggtcc 660 actttaacta ttcgccttag gatagagacg gggacggaac cccctgtggc agtgggaccc 720 aacatagtac tggctgaaca ggggcccccg gccctggagc caccacacaa cttgccggtg 780 ccccagttaa cctcgctgcg gcctgacata acacagccgc ctgacaacag taccactgga 840 ttgatttcct accaacacgc ctagaaactc ccccggtgtt cctgttaaga caggacagag 900 actcttcagt ctcatccagg gagctttcca agccatcaac tccaccgacc ccgatgccag 960 ttcttcttgt tggctttgtc tatcctcagg gcctccttat tatgagggaa tggctagaga 1020 agggaaattc aatgtgacca aagagcatag aaatcaatgt acatgggggt cccgaaataa 1080 gctcaccctc actgaagttt ccgggaaggg gacatgcata ggaaaagctc ccccatccca 1140 ccaacacctt tgcaatagta ctatggttta tgagcaggcc ttagaaaatc agtatttagt 1200 acctggttat aactgatggt gggcatgcaa tactgggtta actccctgtg tttctaccac 1260 agtctttaac caatccaaag atttctgtgt catggtccag ctcgtcccct gggtacacta 1320 ccatcctgag gaagtagtca tcgatgagta tgactatcgg cctacccgct caaaaagaga 1380 gcccgtgacc cttaacccta gccgttatac taggattagg gatggctggt gtggggacag 1440 ggacagctgc cctgatcaca ggaccacagc agctagaaaa aggacttggt gagctacatg 1500 cggccataac agaagatctc cgagccttag aggaatctgt taacaaccta gaagaatccc 1560 tgatttcttt atctgaagtg gttctacaga accgaa 1596 <210> 17 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 17 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctcccttg 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt acgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aataaaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaat tttttagcct catccaggga gcttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaaggca cctgcatagg gatggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcaa 1200 tatctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccttgg ttttcaacca aactaaagac ttttgcgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagagc ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc 1440 gtgggaacag gaacggctgc cctaatcaca ggaccgcaac agctggagaa aggacttagt 1500 aacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaacctg 1560 gaggaatccc taacctcctt atctgaagtg gttctacaga acagga 1606 <210> 18 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 18 atgcatccca cgttaagccg gtgccacctc ccgattcggg gtggagagct gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgct tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccagtac aggtattact attaacagca ctcaagggga ggctccctta 240 gggacctggt ggcctgagtt atatgtctgc cttcgattgg taatccctgg tctcaacgac 300 caggccacac cccctgatgt actctgtgct tacaggttta atgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaagg gagctgcata 420 acttctaatg ataggaattg gaaatggcca atctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaaaat 540 tggcaacagc aggtacaaaa agatgtacga aataagcaaa taagctgtaa tttgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aataatgtac tatggaggct ctgggagaag gaaaggattt 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcta atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 gtggtgaaac tttttagcct catccaggga gattttcagg ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacactcac tgaggtttct ggaaaaggca cctgcatagg gagggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta attgaacctc tgagagtcag 1200 tatctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccctgg ttttcaacca aactaaagac ttttgcttta tggtccaaat tgtcccccgg 1320 gtgtactact atcccaaaaa agcggtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagaac ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc 1440 atgggaacag gaacggctgc cctaatcaca ggaccacaac agctggagaa aggacttagt 1500 gacctacatc gaattgtaat ggaagatctc caagccctag aaaaatctgt cagtaaccta 1560 gaggaatccc taacctcctt gtctgaagtg gttctacaga acagga 1606 <210> 19 <211> 1603 <212> DNA <213> porcine endogenous retrovirus <400> 19 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtagagatct gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgct tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccagtac aggtattact attaacagca ctcaagggga ggctccctta 240 aggacctggt ggcctgagtt atatgtctgc cttggatcgg taatccctgg tctcaacgac 300 caggccacac cccccgatgt actccatgct tacaggtttt atgtttgccc aggaccccca 360 aataataaaa aatactgtga aaatcctcag gatttctttt acaaacaata gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcaac aagacagagt aagttactct 480 tttgttaaca atcctaccac ttataatcaa tttaattatg gccatgggag ataaaaagat 540 tgacaacagc gggtacaaaa agatgtacaa aataagcaaa taagctgtaa tttgttagac 600 ctagattact taaaaataag tttcactgaa aaaaaaaaca agaaaatatt caaaagtggg 660 taaatggtat gtcttgaaaa ataatgtact atggaggctc tgggaaaagg aaaggatcta 720 ttctaactat tcgcctcaga atacaaactc agatggaacc tccggttgct ataggaccaa 780 ataagggttt ggccgaacaa ggacctccaa tccaagaaca gaggccatct cctaacccct 840 ctgattacaa tacaacctct ggatcagtcc ccactgagcc taacatcact attaaaacag 900 gggcgaaact ttttagccta atccagggaa cttttcaagc tcttaactcc acgactccag 960 aggctacctc ttcttgttgg ctttgcttag cttcgggccc accttactat gagaaaatgg 1020 ctagaggagg gaaattcaat gtgacaaaga aacatagaga ccaatgtaca tagggatccc 1080 aaaataagct tacccttact gaggtttctg gaaaagacac ctgcataggg agggttcccc 1140 catcccacca acacctttgt aaccacactg aagcctttaa tcgacctcta agagtcagta 1200 tctggtaccc ggttatgaca ggtggtggac atgtaatact ggattacccc ttgtgtttcc 1260 accctggttt tcaaccaaac taaagacttt tgcgttatgg tccaaattgt cccccgggtg 1320 tactactatc ccgaaaaaac ggtccttgat aaatatgact atagatataa ttggccaaaa 1380 agagaaccca tatccctgac actagctgta atgctcggac tgggagtggc tgcaggcgtg 1440 ggaacaggaa cggctgccct aatcacagga ccacaacagc tagaaaaagg aattagtgac 1500 ctacatcgaa ttgtaacaaa agatctccaa gccctaaaac aatctgtcag taacctggag 1560 gaatccgtaa cctccttgtc tgaagtggtt ctacagaaca gaa 1603 <210> 20 <211> 1639 <212> DNA <213> porcine endogenous retrovirus <400> 20 atgcatccca cgttaagccg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aatgccttat agacagctcg aacccctata gacccttatc ccttacctgg 180 ctgattattg accctagtac aggtattaca atcaacaaca ctcgaggcga ggccccctgg 240 gacacctggt gacctgaact acatgtatgc ctctgatcga taatccccga tcttagagcc 300 acatcccttg acgtaatcca tagttatggg ttttatgtct gtccaggacc cccaaataat 360 gaaaaacttt gcggggaccc cagggatttc ttttgtagga aatggagctg tgttaccttt 420 aacgatgggg attggaaatg gccaacctcc caacaagatt gggtgagctt ctcctttatt 480 aactctttca aagaactttg ggggtcccga agagatatag agggggaaga ttccttatta 540 ggttcatttg atggtcgcaa acaaaacaca gactgttccc cgtcagacct agattatcta 600 aaaataagtt tcactgaaaa aggaaaacag gaaaatattc aaaagtggat aaatggtatg 660 aactggggaa tagttttttt ataaatatgg agggggagca gggtccactt taaccattcg 720 ccttaggata gagacgtgga cggaaccccc tgtggcagtg ggacccgaca aagtactgac 780 tgaacagggc cccggccctg gagccacgca taacttgccg gtgccccaat taacctcgct 840 gcggctgaca taacacagcc gcctggcaac ggtaccactg gattgattcc taccaacacg 900 cctagaaact cccccagtgt tcctgttaag acaggacaga gactcttcag tctcatccag 960 ggagctttcc aagccatcaa ctccaccgac cctgatgcca cttcctcttg ttggctttgt 1020 ctatcctcag ggcctcctta ttatgaggga atggctagag aaggaaaatt caatgtgacc 1080 aaaaaacata gagatcgatg tacacgggta tcccaaaata agcttaccct tactgaggtt 1140 tctggaaaag gcacctacat agggagggtt cccccatccc accaacacct ttgtaaccac 1200 actgaagcct ttaatcgaac ctctgagaat cagtatctgg tacccggtta tgacaggtgg 1260 tgggcatgta atactggatt aagcccttgt gtttccaccc tggttttcga ccaaactaaa 1320 gatttttgcg ttatggtcca aattgtcccc tgggtgtatt accatcccga aaaagcggtc 1380 cttgatgaat atgactatag atataatcgg ccaaaaagag agcccatatc cctgacacta 1440 gctgtaatgc tcagattggg agtggctgca ggcgtgggaa caggaacagc tgccctaatc 1500 acaggaccac gacagttgga gaaaggactt ggtgagctac atagaattat aacggaagat 1560 ctccaagccc tagaaaaatc tgtcaacaac ttagaggaat ccctaacttc tttgtctgaa 1620 gtggttctac agaacagga 1639 <210> 21 <211> 1603 <212> DNA <213> porcine endogenous retrovirus <400> 21 atgcatccca cgttaagccg gcgccacctt ccgattcggg gtagagatct gaaagactga 60 aaatcccctt aagcttcgcc tccatcgctt ggttcctata ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctccctta 240 gggacctggt ggcctgagtt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt atgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattacg gccatgggaa atggaaagat 540 tggcaacagc aggtacaaaa agatgtacga aataagcaaa taagctgtaa tttgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaag gaaaggatct 720 attctaacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagtgtt tggccgaaca aggacctcca atccaagaac agaggccatc tcttaacccc 840 tctgattaca atacagcctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcaaact tttagcctca tccagggagc ttttcaagct cttaactcca caactccaga 960 ggctacctct tcttgttggc tttgcttagc ttcaggccca ccttactatg aggaacggct 1020 agaggaggga aattcaatgt gacaaaggaa catagagacc aatgtacatg gggatcccaa 1080 aataagctta cccttactga ggtttctgga aaaggcacct gcatagggag ggttccccca 1140 tcccaccaac acctttgtaa ccacactgaa gcctttaatc gaacctctga gagtcagtat 1200 ctggtacccg gctatgacag gtggtgggca tgtaatactg gattaacccc ttgtgtttcc 1260 accctggttt tcaaccaaac taaagacttt tgcgttatgg tccaaattgt cccccgggtg 1320 tactactatc ccgaaaaagc ggtccttgat gaatatgact atagatataa tcagccaaaa 1380 agagaaccca tatccctgac actagctgta atgctcggat tgggagtggc tgcaggcgtg 1440 ggaacaggaa cggctgccct aatcacagga ccacaacagc tggagaaagg acttagtgac 1500 ctacatcgaa ttgtaacgga agatctccaa gccctagaaa aatctgtcag taacctagag 1560 gaatccctaa cctccttatc tgaagtggtt ctacagaaca gaa 1603 <210> 22 <211> 1637 <212> DNA <213> porcine endogenous retrovirus <400> 22 atgcatccca cgttaagccg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aatgccttat agacagctcg aacccctata gacccttatc ccttacctgg 180 ctgattattg accctagtac aggtattaca atcaacaaca ctcgaggcga ggccccctgg 240 gacacctggt gacctgaact acatgtatgc ctctgatcga taatccccga tcttagagcc 300 acatcccttg acgtaatcca tagttatggg ttttatgtct gtccaggacc cccaaataat 360 gaaaaacttt gcggggaccc cagggatttc ttttgtagga aatggagctg tgttaccttt 420 aacgatgggg attggaaatg gccaacctcc caacaagatt gggtgagctt ctcctttatt 480 aactctttca aagaactttg ggggtcccga agagatatag agggggaaga ttccttatta 540 ggttcatttg atggtcgcaa acaaaacaca gactgttccc cgtcagacct agattatcta 600 aaaataagtt tcactgaaaa aggaaaacag gaaaatattc aaaagtggat aaatggtatg 660 aactggggaa tagtttttta taaatatgga gggggagcag ggtccacttt aaccattcgc 720 cttaggatag agacgtggac ggaaccccct gtggcagtgg gacccgacaa agtactgact 780 gacaggggcc cccggccctg gagccccgca taacttgccg gtgcccaatt aactcgctgc 840 ggcctgacat aacacagccg cctggcaacg gtaccactgg attgattcct accaacacgc 900 ctagaactcc cccagtgttc ctgttaagac aggacagaga ctcttcagtc tcatccaggg 960 agctttccaa gccatcaact ccaccgaccc tgatgccact tcctcttgtt ggctttgtct 1020 atcctcaggg cctccttatt atgagggaat ggctagagaa ggaaaattca atgtgaccaa 1080 aaaacataga gatcgatgta cacgggtatc ccaaaataag cttaccctta ctgaggtttc 1140 tggaaaaggc acctacatag ggagggttcc cccatcccac caacaccttt gtaaccacac 1200 tgaagccttt aatcgaacct ctgagaatca gtatctggta cccggttatg acaggtggtg 1260 ggcatgtaat actggattaa gcccttgtgt ttccaccctg gttttcgacc aaactaaaga 1320 tttttgcgtt atggtccaaa ttgtcccctg ggtgtattac catcccgaaa aagcggtcct 1380 tgatgaatat gactatagat ataatcggcc aaaaagagag cccatatccc tgacactagc 1440 tgtaatgctc agattgggag tggctgcagg cgtgggaaca ggaacagctg ccctaatcac 1500 aggaccacga cagttggaga aaggacttgg tgagctacat agaattataa cggaagatct 1560 ccaagcccta gaaaaatctg tcaacaactt agaggaatcc ctaacttctt tgtctgaagt 1620 ggttctacag aacagga 1637 <210> 23 <211> 1597 <212> DNA <213> porcine endogenous retrovirus <400> 23 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcggggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacagaac cccctgtggc agtgggaccc 720 gataaagtac tggctgaaca ggggcccccg gccctggagc caccgcataa cttgccggtg 780 ccccaattaa cctcgctgcg gcctgacata acacagccgc ctagcaacag taccactgga 840 ttgattccta ccaacacgcc tagaaactcc ccaggtgttc ctgttaagac aggacagaga 900 ctcttcagtc tcatccaggg agctttccaa gccatcaact ccaccgaccc tgatgccact 960 tcttcttgtt ggctttgtct atcctcaggg cctccttatt atgaggggat ggctaaagaa 1020 ggaaaattca atgtgaccaa agagtataga aatcaatgta catgggggtc ccgaaataag 1080 cttaccctca ctgaagtttc cgggaagggg acatgcatag gaaaagctcc cccatcccac 1140 caacaccttt gctatagtac tgtggtttat gagcaggcct cagaaaatca gtatttagta 1200 cctggttata acaggtggtg ggcatgcaat actgggttaa ccccctgtgt ttccacctca 1260 gtcttcaacc aatccaaaga tttctgtgtc atggtccaaa tcgtcccccg agtgtactac 1320 catcctgagg aagtggtcct tgatgaatat gactatcggt ataaccgacc aaaaagagaa 1380 cccgtatccc ttaccctagc tgtaatgctc ggattaggga cggccgttgg cgtaggaaca 1440 gggacagctg ccctgatcac aggaccacag cagctagaga aaggacttgg tgagctacat 1500 gcggccatga cagaagatct ccgagcctta gaggagtctg ttagcaacct agaagagtcc 1560 ctgacttctt tgtctgaagt ggttctacag aaccgaa 1597 <210> 24 <211> 1593 <212> DNA <213> porcine endogenous retrovirus <400> 24 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa acaggaaaat attcaaaagt 600 ggataaatgg tatgagctgg ggaatagttt tttataaata tggcggggga gcagggtcca 660 ctttaaccat tcgccttagg atagagacgg ggacagaacc ccctgtggca gtgggacccg 720 ataaagtact ggctgaacag gggcccccgg ccctggagcc accgcataac ttgccggtgc 780 cccaattaac ctcgctgcgg cctgacataa cacagccgcc tagcaacggt accactggat 840 tgattcctac caacacgcct agaaactccc aggtgttcct gttaagacag gacagagact 900 cttcagtctc atccagggag ctttccaagc catcaactcc accgaccctg atgccacttc 960 ttcttgttgg ctttgtctat cctcaggccc tccttattat gaggggatgg ctaaagaagg 1020 aaaattcaat gtgaccaaag agcatagaaa tcaatgtaca tggggtccga aataagctta 1080 ccctcactga aatttccggg aaggggacat gcataggaaa agctccccca tcccaccaac 1140 acctttgcta tagtactgtg gtttatgagc aggcctcaga aaatcagtat ttagtacctg 1200 gttgtaacag gtggtgggca cgcaatactg ggttaacccc ctgtgtttcc acctcagtct 1260 tcaaccaatc caaagatttc tgtgtcatgg tccaaatcgt cccccgagtg tactaccatc 1320 ctgaggaagt ggtccttgat gaatatgact atcggtataa ccgaccaaaa agagaacccg 1380 tatccctgac actagctgta atgctcggac tgggagtggc tgcaggcgtg ggaacaggaa 1440 cggctgccct aatcacagga ccacaacagc tagaaaaagg aattagtgac ctacatcgaa 1500 ttgtaacaaa agatctccaa gccctaaaac aatctgtcag taacctagag gaatccctaa 1560 cctccttgtc tgaagtggtt ctacagaaca gaa 1593 <210> 25 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 25 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctcccttg 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt acgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaac tttttaacct catccaggga gcttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaaggca cctgcatagg gatggttccc 1140 ccatcccacc aacacctttg tagccacact gaagccttta atcgaacctc tgagagtcag 1200 tatctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccttgg ttttcaacca aactaaagac ttttgcgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagagc ccatatccct gacactagct gtaatgctcg gattgggaat ggctgcaggc 1440 gtgggaacag gaacggctgc cctaatcaca ggaccgcaac agctggagaa aggacttagt 1500 aacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaacctg 1560 gaggaatccc taacctcctt atctgaagtg gttctacaga acagga 1606 <210> 26 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 26 atgcatccca cgttaagccg gcgccccctc ccgattcggg gtagagatct gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgct tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctccctta 240 gggacctggt ggcctgagtt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt atgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattacc gccatgggaa atggaaagat 540 tggcaacagc aggtacaaaa agatgtacga aataagcaaa taagctgtaa tttgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa ggaaaggatc 720 tattctaact attcgcctca gaatagaaac tcagatggaa cctccggttg ctataggacc 780 aaataagtgt ttggccgaac aaggacctcc aatccaagaa cagaggccat ctcttaaccc 840 ctctgattac aatacagcct ctggatcagt ccccactgag cctaacatca ctattaaaac 900 aggggcaaaa ctttttagcc tcatccaggg agcttttcaa gctcttaact ccacaactcc 960 agaggctacc tcttcttgtt ggctttgctt agcttcaggc ccaccttact atgaggaacg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaaggca cctgcatagg gagggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag 1200 tatctggtac ccggctatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccctgg ttttcaacca aactaaagac ttttgcgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcggtcctt gatgaatatg actatagata taatcagcca 1380 aaaagagaac ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc 1440 gtgggaacag gaacggctgc cctaatcaca ggaccacaac agctggagaa aggacttagt 1500 gacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaaccta 1560 gaggaatccc taacctcctt atctgaagtg gttctacaga acagaa 1606 <210> 27 <211> 1597 <212> DNA <213> porcine endogenous retrovirus <400> 27 atgcatccca cgttaagctg gcaccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcgct gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacagaac cccctgtggc agtgggaccc 720 gataaagtac tggctgaaca ggggcccccg gccctggagc caccgcataa cttgccggtg 780 ccccaattaa cctcgctgcg gcctgacata acacagccgc ctagcaacag taccactgga 840 ttgattccta ccaacacgcc tagaaactcc ccaggtgttc ctgttaagac aggacagaga 900 ctcttcagtc tcatccaggg agctttccaa gccatcaact ccaccgaccc tgatgccact 960 tcttcttgtt ggctttgtct atcctcaggg cctccttatt atgaggggat ggctaaagaa 1020 ggaaaattca atgtgaccaa agagcataga aatcaatgta catgggggtc ccgaaataag 1080 cttaccctca ctgaagtttc cgggaagggg acatgcatag gaaaagctcc cccatcccac 1140 caacaccttt gctatagtac tgtggtttat gagcaggcct cagaaaatca gtatttagta 1200 cctggttata acaggtggtg ggcatgcaat actgggttaa ccccctgtgt ttccacctca 1260 gtcttcaacc aatccaaaga tttctgtgtc atggtccaaa tcgtcccccg agtgtactac 1320 catcctgagg aagtggtcct tgatgaatat gactatcggt ataaccgacc aaaaagagaa 1380 cccgtatccc ttaccctagc tgtaatgctc ggattaggga cggccgttgg cgtaggaaca 1440 gggacagctg ccctgatcac aggaccacag cagctagaga aaggacttgg tgagctacat 1500 gcggccatga cagaagatct ccgagcctta gaggagtctg ttagcaacct agaagagtcc 1560 ctgacttctt tgtctgaagt ggttctacag aaccgaa 1597 <210> 28 <211> 1609 <212> DNA <213> porcine endogenous retrovirus <400> 28 atgcatccca cgttaaggcg gcgccacctc ccgattcggg gtggagagcc gaaaaaactg 60 aaaatcccct taagcttcgc ctccatcgct tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgc ggacagcccg aacttccata aacccttatc tctcacctgg 180 ttacttactg actccagtac aggtattact attaacagca ctcaagggga ggctccctta 240 gggacctggt ggcctgagtt atatgtctgc cttcgatcgg taatccctgg tctcaacgac 300 caggccacac cccctgatgt actccgtgct tacgggttta atgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaagg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca gtcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtaa ttcgttagat 600 ctagattact taaaaataag ttttactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aataatgtac tatggaggca ctgggagaag gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggctgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgtttaca atacaacctc tggattagtc ccccctgagc ctaacttcac tattaaaaca 900 ggggcgaaac tttttagcct catccaggga gcttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gctttggggc ccaccttact atgagggaat 1020 ggctagagga gggaaattca atgtgacaaa ggaacataga gaccaatgta catggggatc 1080 ccaaaataag cttaccctta ctgaggtttc tggaaaaggc acctgaatag ggagggttcc 1140 cccatcccac caacaccttt gtaaccacac tgaagccttt aatcgaacct ctgagagtca 1200 gtacctggta cctggttatg acaggtggtg ggcatgtaat actggattaa ccccttgtgt 1260 ttccaccctg gttttcaacc aaactaaaga cttttgtgtt atggtccaaa ttgtcccccg 1320 ggtgtactac tatcccgaaa aagcagtcct tgatgaatat gactatggat ataatcggcc 1380 aaaaagagaa cccatatccc tgacactagc tgtaatgctc ggattgggag tggctgcagg 1440 cgtggaaaca ggaacggctg ccctaatcac aggaccacaa cagctggaga aaggacttag 1500 tgacctacat cgaattgtaa cggaagatct ccaagcccta gaaaaatctg tcagtaacct 1560 agaggaatcc gcataacctc cttatctgaa gtggttctac agaacagga 1609 <210> 29 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 29 atgcatccca cgttaagccg gcgccccctc ccgattcggg gtggagagcc gaaaaaactg 60 aaaatcccct taagcttcgc ctccatcgct tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgc ggacagcccg aacttccata aacccttatc tctcacctgg 180 ttacttactg actccagtac aggtattact attaacagca ctcaagggga ggctccctta 240 gggacctggt ggcctgagtt atatgtctgc cttcgatcgg taatccctgg tctcaacgac 300 caggccacac cccctgatgt actccgtgct tacgggttta atgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaagg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca gtcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtaa ttcgttagat 600 ctagattact taaaaataag ttttactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aataatgtac tatggaggca ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggctgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgtttaca atacaacctc tggattagtc ccccctgagc ctaacttcac tattaaaaca 900 ggggcgaaac tttttagcct catccaggga gcttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gctttgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaaggca cctgcatagg gagggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag 1200 tacctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccctgg ttttcaacca aactaaagac ttttgtgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagaac ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc 1440 gtggaaacag gaacggctgc cctaatcaca ggaccacaac agctggagaa aggacttagt 1500 gacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaaccta 1560 gaggaatccc taacctcctt atctgaagtg gttctacaga accgga 1606 <210> 30 <211> 1597 <212> DNA <213> porcine endogenous retrovirus <400> 30 atgcatccca cgttaagctg gcgccacgtc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccctgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacggaac cccctgtggc agtgggaccc 720 gataaagtac tggctgaaca ggggcccccg gccctggagc caccgcataa cttgccggtg 780 ccccaattaa cctcgctgcg gcctgacata acacagccac ctagcaacgg taccactgga 840 ttgatttcta ccaacacgcc tagaaactcc ccaggtgttc ctgttaagac aggacagaga 900 cccttcagtc tcatccaggg agctttccaa gccatcaact ccaccgaccc cgatgccact 960 tcttcttgtt ggctttgtct atcctcaggg cctccttatt atgaggggat ggctaaagaa 1020 ggaaaattca atgtgaccaa agagcataga aatcaatgta catgggggtc ccgaaataag 1080 cttaccctca ctgaagtttc cgggaagggg acatgcatag gaaaagctcc cccatcccac 1140 caacaccttt gctatagtac tgtggtttat gagcaggcct cagaaaatca gtatttagta 1200 cctggttata acaggtggtg ggcatgcaat actgggttaa ccccctgtgt ttccacctca 1260 gtcttcaacc aatccaaaga tttctgtgtc atggtccaaa tcgtcccccg agtgtactac 1320 catcccgagg aagtggtcct tgatgaatat gactatcggt ataaccgacc gaaaagagaa 1380 cccgtatccc ttaccctagc tgtaatgctc ggattaggga cggccgttgg tgtaggaaca 1440 ggggcagctg ccctgatcac aggaccacag cagctagaga aaggacttgg tgagctacgt 1500 gcggccatga cagaagatct ccgagcctta gaggaatctg ttagcaacct agaagagtcc 1560 ctgacttctt tgtctgaagt ggttctacag aaccgaa 1597 <210> 31 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 31 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggagagcc gaaaaaactg 60 aaaatcccct taagcttcgc ctccatcgct tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgc ggacagcccg aacttccata aacccttatc tctcacctgg 180 ttacttactg actccagtac aggtattact attaacagca ctcaagggga ggctccctta 240 gggacctggt ggcctgagtt atatgtctgc cttcgatcgg taatccctgg tctcaacgac 300 caggccacac cccctgatgt actccgtgct tacgggttta atgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaagg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca gtcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtaa ttcgttagat 600 ctagattact taaaaataag ttttactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aataatgtac tatggaggca ctgggagaag gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggctgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgtttaca atacaacctc tggattagtc ccccctgagc ctaacttcac tattaaaaca 900 ggggcgaaac tttttagcct catccaggga gcttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gctttgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaaggca cctgcatagg gagggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag 1200 tacctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccctgg ttttcaacca aactaaagac ttttgtgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagaac ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc 1440 gtggaaacag gaacggctgc cctaatcaca ggaccacaac agctggagaa aggacttagt 1500 gacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaaccta 1560 gaggaatccc taacctcctt atctgaagtg gttctacaga accgga 1606 <210> 32 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 32 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttactta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctcccttg 240 gggacctgat ggcctgagtt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt acgtttgccc aggaccccca 360 aataatgaaa aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt gagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tgacaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaagaaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagagggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggaacgaaac tttttagcct catccaggga gcttttcaag ctcttaacta cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct gaaaaaggca cctgcatagg gagggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcaa 1200 tatctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccttgg ttttcaacca aactaaagac ttttgcgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagagc ccatatccct gacactagct gtaatgctcg gattgggagt agctacaggc 1440 gtgggaacag gaacggctgc cctaatcaca ggaccgcaac agctggagaa aggacttagt 1500 aacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaacctg 1560 gaggaatccc taacctcctt atctgaagtg gttctacaga acagga 1606 <210> 33 <211> 1590 <212> DNA <213> porcine endogenous retrovirus <400> 33 atgcatccca cgttaagctg gcgccacgtc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccctgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacggaac cccctgcggc agtgggaccc 720 gataaagtac tggctgaaca ggggcccccg gccctggagc caccgcataa cttgccggtg 780 ccccaattaa cctcgctgcg gcctgacata acacagccac ctagcaacgg taccactgga 840 ttgatttcta ccaacacgcc tagaaactcc caggtgtcct gttaagacag gacagagacc 900 cttcagtctc atccagggag ctttccaagc catcaactcc accgaccccg atgccacttc 960 ttcttgttgg ctttgtctat cctcagggcc tccttattat gaggggatgg ctaaaaaagg 1020 aaaattcaat gtgaccaaag agcatagaaa tcaatgtaca tgggggtccc gaaataagct 1080 taccctcact gaagtttccg ggaaggggac atgcatagga aaagctcccc catcccacca 1140 acacctttgc tatagtactg tggtttatga gcaggcctca gaaaatcagt atttagtacc 1200 tggttataac aggtggtggg catgcaatac tgggttaacc ccctgtgttt ccacctcagt 1260 cttcaaccaa tccaaagatt tctgtgtcat ggtccaaatc gtcccccgag tgtactacca 1320 tcccgaggaa gtggtccttg atgaatatga ctatcggtat aaccgaccga aaagagaacc 1380 cgtatccctt accctagctg taatgctcgg attagggacg gccgttggtg taggaacagg 1440 gacagctgcc ctgatcacag gaccacagca gctagagaaa ggacttggtg agcgacatgc 1500 ggccatgaca gaagatctcc gagcctagag gaatctgtag cacgtagaag agtcgtgatt 1560 ctttgtctga agtggttcta cagaaccgga 1590 <210> 34 <211> 1605 <212> DNA <213> porcine endogenous retrovirus <400> 34 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctcccttg 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt acgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaac tttttaacct catccaggga gctttcaagc tcttaactcc acgactccag 960 aggctacctc ttcttgttgg ctttgcttag cttcgggccc accttactat gagggaatgg 1020 ctagaggagg gaaattcaat gtgacaaagg aacatagaga ccaatgtaca tggggatccc 1080 aaaataagct tacccttact gaggtttctg gaaaaggcac ctgcataggg atggttcccc 1140 catcccacca acacctttgt aaccacactg aagcctttaa tcgaacctct gagagtcagt 1200 atctggtacc tggttatgac aggtggtggg catgtaatac tggattaacc ccttgtgttt 1260 ccaccttggt tttcaaccaa actaaagact tttgcgttat ggtccaaatt gtcccccggg 1320 tgtactacta tcccgaaaaa gcagtccttg atgaatatga ctatagatat aatcggccaa 1380 aaagagagcc catatccctg acactagctg taatgctcgg attgggagtg gctgcaggcg 1440 tgggaacagg aacggctgcc ctaatcacag gaccgcaaca gctggagaaa ggacttagta 1500 acctacatcg aattgtaacg gaagatctcc aagccctaga aaaatctgtc agtaacctgg 1560 aggaatccct aacctccttg tctgaagtgg ttctacagaa ccgga 1605 <210> 35 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 35 atgcatccca cgttaagccg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcg tggttcctta ctctaacagt gactccccag 120 gccagtagta aacgccttat agacagctcg gacccccata gacccttatc ccttacgtgg 180 ctgattattg accctgatac gggtgtcact gttaatagca ctcgaggtgt cgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 gaatactgtg ggggttccag ggaatccttc tgtgggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatggaa ctatataaag ataagagctg ctctccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggaggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacgagaa ccccctgtgg cagtgggacc 720 caacatagta ctggctgaac aggggccccc ggccctggag ccaccacaca acttgccggt 780 gcccccagtt aacctcgctg cggcctgaca taacaccagc cgcctgacaa cagtatcact 840 ggattgattt cctaccaaca cgcctagaaa ctccccccgg tgttcctgtt aagacaggac 900 agagactctt cagtctcatc cagggagctt tccaagccat caactccacc gaccccgatg 960 ccacttcttc ttgttggcct ttgttctatc ctcaagggcc tccttattat gaggggaatg 1020 gctagagaat gggaaatttc aatgtgacca aagagcatag aaatcaatgt acatgggggt 1080 cccgaaataa gctcaccctc actgaagttt ccgggaaggg gacatgcata ggaaaagctc 1140 ccccatccca ccaacacctt tgcaatagta ctatggttta tgagcaggcc ttagaaaatc 1200 agtatttagt acctggttat aactgatggt gggcatgcaa tactgggtta actccctgtg 1260 tttctaccac agtctttaac caatccaaag atttctgtgt catggtccag ctcgtcccct 1320 gggtacacta ccatcctgag gaagtagtca tcgatgagta tgactatcgg cctacccgct 1380 caaaaagaga gcccgtgacc cttaacccta gccgttatac taggattagg gatggctggt 1440 gtggggacag ggacagctgc cctgatcaca ggaccacagc agctagaaaa aggacttggt 1500 gagctacatg cggccataac agaagatctc cgagccttag aggaatctgt taacaaccta 1560 gaagctctat agacttcttt atctgaagtg gttctacaga acagga 1606 <210> 36 <211> 1601 <212> DNA <213> porcine endogenous retrovirus <400> 36 atgcatccca cgttaagccg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacagt gactccccag 120 gccagtagta aacgccttat agacagctcg gacccccata gacccttatc ccttacgtgg 180 ctgattattg accctgatac gggtgtcact gttaatagca ctcgaggtgt cgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 gaatactgtg ggggttccag ggaatccttc tgtgggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatggaa ctatataaag ataagagctg ctctccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggaggggg gagcagggtc 660 cacttttacc atttcgcctt taggatagag acggggacgg aaccccctgt ggcagtggga 720 cccaacatag tactggctga acaggggccc ccggccctgg agccaccaca caacttgccg 780 gtgccccaat taacctcgct gcggcctgac ataacacagc cgcctgacaa cagtaccact 840 ggattgattt ctaccaacac gcctagaaac tccccggtgt tcctgttaag acaggacaga 900 gactcttcag tctcatcgag ggagctttcc aagccatcaa ctccaccgac cccgatgcca 960 cttcttcttg ttggctttgt ctatcctcag ggcctcctta ttatgagggg aatggctaga 1020 gaaggggaaa ttcaatgtga ccaaagagca tagaaatcaa tgtacatggg ggtcccgaaa 1080 taagctcacc ctcactgaag tttccgggaa ggggacatgc ataggaaaag ctcccccatc 1140 ccaccaacac ctttgcaata gtactatggt ttatgagcag gccttagaaa atcagtattt 1200 agtacctggt tataactgat ggtgggcatg caatactggg ttaactccct gtgtttctac 1260 cacagtcttt aaccaatcca aagatttctg tgtcatggtc cagctcgtcc cctgggtaca 1320 ctaccatcct gaggaagtag tcatcgatga gtatgactat cggcctaccc gctcaaaaag 1380 agaacccgtg acccttaacc ctagccgtta tactaggatt agggatggct ggtgtgggga 1440 cagggacagc tgccctgatc acaggaccac agcagctaga aaaaggactt ggtgtaagct 1500 rcatgcggcc ataacagaag atctcamgag ccttagagga atctgtaaca acctagaaga 1560 atgcgtgact tctttatctg aagtggttct acagaacagg a 1601 <210> 37 <211> 1597 <212> DNA <213> porcine endogenous retrovirus <400> 37 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgtgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaagaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacagaac cccctgtggc agtgggaccc 720 gataaagtac tggctgaaca ggggcccccg gccctggagc caccgcataa cttgccggtg 780 ccccaattaa cctcgctgcg gcctgacata acacagccgc ctagcaacgg taccactgga 840 ttgattccta ccaacacgcc tagaaactcc ccaggtgttc ctgttaagac aggacagaga 900 ctcttcagtc tcatccaggg agctttccaa gccatcaact ccaccgaccc tgatgccact 960 tcttcttgtt ggctttgtct atcctcaggg cctccttatt atgaggggat ggctaaagaa 1020 ggaaaattca atgtgaccaa agagcataga aatcaatgta catgggggtc ccaaaataag 1080 cttaccctca ctgaagtttc cgggaagggg acatgcatag gaaaagctcc cccatcccac 1140 caacaccttt gctatagtac tgtggtttat gagcaggcct cagaaaatca gtatttagta 1200 cctggttata acaggtggtg ggcatgcaat actgggttaa ccccctgtgt ttccacctca 1260 gtcttcaacc aatccaaaaa tttctgtgtc atggtccaaa tcgtcccccg agtgtactac 1320 catcctgaga aagtggtcct tgatgaatat gactatcggt ataaccgacc aaaaagagaa 1380 cccgtatccc ttaccctagc tgtaatgctc ggattaggga cggccgttgg cgtaggaaca 1440 gggacagctg ccctgatcac aggaccacag cagctagaga aaggacttgg tgaactacat 1500 gcggccatga cagaagatct ccgagcctta gaggagtctg ttagcaacct agaagagtcc 1560 ctgacttctt tgtctgaagt ggttctacag aaccgga 1597 <210> 38 <211> 1588 <212> DNA <213> porcine endogenous retrovirus <400> 38 atgcatccca cgttaagccg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacagt gactccccag 120 gccagtagta aacgccttat agacagctcg gacccccata gacccttatc ccttacgtgg 180 ctgattattg accctgatac gggtgtcact gttaatagca ctcgaggtgt cgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttccag ggaatccttc tgtgggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatggaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggaggggg agcagggtcc 660 acttttaact attcgcctta ggatagagac gggacggaac ccctgtggca gtgggaccca 720 acatagtact ggctgaacag gggccctggc cctggagcca ccacacaact tgccggtgcc 780 cagttaacat cgctgcggcc tgacataaca cagccgcctg acacagtacc actggattga 840 ttcctaccaa cacgcctaga aactcccccg gtgttcctgt taagacagga cagagactct 900 tcagtctcat ccaggagctt tccaagccat cactccaccg acccgatgcc acttcttctt 960 gttggctttg tctatcctca gggcctcctt attatgagga aatggctaga gaagggaaat 1020 tcaatgtgac caaagagcat agaaatcaat gtacatgggg gtcccgaaat aagctcaccc 1080 tcactgaagt ttccgggaag gggacatgca taggaaaagc tcccccatcc caccaacacc 1140 tttgcaatag tactatggtt tatgagcagg ccttagaaaa tcagtattta gtacctggtt 1200 ataactgatg gtgggcatgc aatactgggt taactccctg tgtttctacc acagtcttta 1260 accaatccaa agatttctgt gtcatggtcc agctcgtccc ctgggtacac taccatcctg 1320 aggaagtagt catcgatgag tatgactatc ggcctacccg ctcaaaaaga gagcctgtga 1380 cccttaaccc tagccgttat actaggatta gggatggctg gtgtggggac agggacagct 1440 gccctgatca caggaccaca gcagctagaa acaggacttg gtgagctaca tgcggccata 1500 acagaagatc tccgagcctt agaggaatct gttaacaacc tagaagagtc cctgacttct 1560 ttatctgaag tggttctaca gaaccgga 1588 <210> 39 <211> 1594 <212> DNA <213> porcine endogenous retrovirus <400> 39 atgcatccca cgttaagccg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacagt gactccccag 120 gccagtagta aacgccttat agacagctcg gacccccata gacccttatc ccttacgtgg 180 ctgattattg accctgatac gggtgtcact gttaatagca ctcgaggtgt cgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 gaatactgtg ggggttccag ggaatccttc tgtgggagat ggagctgtgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatggaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggaggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacggaac cccctgtggc agtgggaccc 720 aacatagtac tggctgaaca ggggccccgg ccctggagcc accacacaac ttgccggtgc 780 cccagttaac ctcgctgcgg cctgacataa cacagccgcc tgacaacagt accactggat 840 tgatttccta ccaacacgcc tagaaactcc ccggtgttcc tgttaagaca ggacagagac 900 tcttcagtct catccaggga gctttccaag ccatcaactc caccgacccc gatgccactt 960 cttcttgttg gctttgtcta tcctcagggc ctccttatta tgagggaatg gctagagaag 1020 ggaaattcaa tgtgaccaaa gagcatagaa atcaatgtac atgggggtcc cgaaataagc 1080 tcaccctcac tgaagtttcc gggaaggggg catgcatagg aaaagctccc ccatcccacc 1140 aacacctttg caatagtact atggtttatg agcaggcctt agaaaatcag tatttagtac 1200 ctggttataa ctgatggtgg gcatgcaata ctgggttaac tccctgtgtt tctaccacag 1260 tctttaacca atccaaagat ttctgtgtca tggtccagct cgtcccctgg gtacactacc 1320 atcctgagga agtagtcatc gatgagtatg actatcggcc tacccgctca aaaagagagc 1380 ccgtgaccct taaccctagc cgttatacta ggattaggga tggctggtgt ggggacaggg 1440 acagctgccc tgatcacagg accacagcag ctagaaaaag gacttggtga gctacatgcg 1500 gccataacag aagatctccg agccttagag gaatctgtta acaacctaga agaatctaag 1560 gcttctttgt ctgaagtggt tctacagaac agga 1594 <210> 40 <211> 1596 <212> DNA <213> porcine endogenous retrovirus <400> 40 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggagagcc gaaaagactg 60 aaaaccccct taagcttcgc ctccatcgct tggttcctta ctctgtaaat aactcctcaa 120 gttaatggta aacgcctgac agcgaactcc cacaaaccct tatctctcac ctggttactt 180 actgactcca gtacaggtat tactattaac agcactcaag gggaggctcc cttagggacc 240 tggtggcctg agttatatgt ctgccttcga tcggtaatcc ctggtctcaa cgaccaggcc 300 acaccccccg atgtactccg tgcttagggg ttttatgttt gcccaggacc cccaaataat 360 gaagaatatt gtggaaatcc tcaggatttc ttttgcaagc aatggagctg cgtaacttct 420 aatgatggga attggaaatg gccagtctct cagcaagaca gagtaagtta ctcttttgtt 480 aacaatccta ccagttataa tcaatttaat taccgccatg tgaaatggaa agattggcaa 540 cagcaggtac aaaaagatgt acgaaataag caaataagct gtatttgtta gacctagatt 600 acttaaaaat aagtttcact gaaaaggaaa caagaaaata tcaaaagtgg gtaaatggta 660 tgtcttgggg aatagtgtac tatggaggct ctgggagaag gaaaggatct attctaacta 720 ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca aataagtgtt 780 tggccgaaca aggacctcca atccaagaac agaggccatc tcttaacccc tctgattaca 840 atacagcctc tggatcagtc cccactgagc ctaacatcac tattaaaaca ggggcaaaac 900 tttttagcct catccaggga gcttttcaag ctcttaactc cacaactcca gaggctacct 960 cttcttgttg gctttgctta gcttcaggcc caccttacta tgagggaatg gctagaggag 1020 ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc caaaataagc 1080 ttacccttac tgaggtttct ggaaaaggca cctgcatagg gagggttccc ccatcccacc 1140 aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag tatctggtac 1200 ccggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt tccaccctgg 1260 ttttcaacca aactaaagac ttttgcgtta tggtccaaat tgtcccccgg gtgtactact 1320 atcccgaaaa agcggtcctt gatgaatatg actatagata taatcagcca aaaagagaac 1380 ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc gtgggaacag 1440 gaacggctgc cctaatcaca ggaccacaac agctggagaa aggacttagt gacctacatc 1500 gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaaccta gaggaatccc 1560 taacctcctt gtctgaagtg gttctacaga acagga 1596 <210> 41 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 41 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga agctcccttg 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt acgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaac tttttaacct catccaggga gcttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaaggca cctgcatagg gatggttccc 1140 ccatcccacc aacacctttg taaccacact aaagccttta atcgaacctc tgagagtcag 1200 tatctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccttgg ttttcaacca aactaaagac ttttgcgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagagc ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc 1440 gtgggaacag gaacggctgc cctaatcaca ggaccgcaac agctggagaa aggacttagt 1500 aacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaaccta 1560 gaggaatccc taacctcctt gtctgaagtg gttctacaga accgga 1606 <210> 42 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 42 atgcatccca cgttaagccg gcgccatcac ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggaa ggctccctta 240 gggacctggt ggcctgagtt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt atgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagg gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccgattgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaac tttttagcct catccaggga gcttttcaag ctcttaactc caagactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaaggca cctgcatagg gagggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcaa 1200 tatctggtac ctggttatgg caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccacgttgg ttttaaacca aactaaagac ttttgtgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taattggcca 1380 aaaagagaac ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc 1440 gtgggaacag gaacggctgc cctaatcaca ggaccacaac agctggagaa aggacttagt 1500 aacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaacctg 1560 gaggaatccc taacctcctt atctgaagtg gttctacaga accgaa 1606 <210> 43 <211> 1597 <212> DNA <213> porcine endogenous retrovirus <400> 43 atgcatccca cgttaagctg gtgccacgtc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagctttgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccctgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agaaaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacggaac cccctgtggc agtgggaccc 720 gataaagtac tggctgaaca ggggccccgg gccctggagc caccgcataa cttgccggtg 780 ccccaattaa cctcgctgcg gcctgacata acacagccac ctagcaacgg taccactgga 840 ttgattccta ccaacacgcc tagaaactcc ccaggtgttc ctgttaagac aggacagaga 900 cccttcagtc tcatccaggg agctttccaa gccatcaact ccaccgaccc cgatgccact 960 tcttcttgtt ggctttgtct atcctcaggg cctccttatt atgaggggat ggctaaaaaa 1020 ggaaaattca atgtgaccaa agagcataga aatcaatgta catgggggtc ccgaaataag 1080 cttaccctca ctgaagtttc cgggaagggg acatgcatag gaaaagctcc cccatcccac 1140 caacaccttt gctatagtac tgtggtttat gagcaggcct cagaaaatca gtatttagta 1200 cctggttata acaggtggtg ggcatgcaat actgggttaa ccccctgtgt ttccacctca 1260 gtcttcaacc aatccaaaga tttctgtgtc atggtccaaa tcgtcccccg agtgtactac 1320 catcccgagg aagtggtcct tgatgaatat gactatcggt ataaccgacc gaaaagagaa 1380 cccgtatccc ttaccctagc tgtaatgctc ggattaggga cggccgttgg tgtaggaaca 1440 gggacagctg ccctgatcac aggaccacag cagctagaaa aaggacttgg tgagctacat 1500 gcggccatga cagaagatct ccgagcctta gaggaatctg ttagcaacct agaagattcc 1560 ctgacttctt tgtctgaagt ggttctacag aacagga 1597 <210> 44 <211> 1602 <212> DNA <213> porcine endogenous retrovirus <400> 44 atgcatccca cgttaagtcg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttgcttactg actccggtac aggtattaat attaacagca ctcaagggga ggctcccttg 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt acgtttgccc aggaccccca 360 aataatgaag aatattgtga aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aataaaactc agatggaacc tccggttgct ataggaccaa 780 ataagggttt ggccgaacaa ggacctccaa tccaagaaca gaggccatct cctaacccct 840 ctgattacaa tacaacctct ggatcagtcc ccactgagcc taacatcact attaaacagg 900 ggcgaaactt ttaactcatc cagggagctt ttcaagctct taactccacg actccagagg 960 ctacctcttc ttgttggctt tgcttagctt cgggcccacc ttactatgag ggaatggcta 1020 gaggagggaa attcaatgtg acaaaggaac atagagacca atgtacatgg ggatcccaaa 1080 ataagcttac ccttactgag gtttctggaa aaggcacctg catagggatg gttcccccat 1140 cccaccaaca cctttgtaac cacactgaag cctttaatcg aacctctgag agtcagtatc 1200 tggtacctgg ttatgacagg tggtgggcat gtaatactgg attaacccct tgtgtttcca 1260 ccttggtttt caaccaaact aaagactttt gcgttatgat ccaaattgtc ccccgggtgt 1320 actactatcc cgaaaaagca gtccttgatg aatatgacta tagatataat cggccaaaaa 1380 gagagcccat atccctgaca ctagctgtaa tgctcggatt gggagtggct gcaggcgtgg 1440 gaacaggaac ggctgccaat aatcacagga ccgcaacagc tggagaaagg acttagtgaa 1500 ctgcatcgaa tgtaacggaa gatctccaag ccctagaaaa atctgtcagt aacctggagg 1560 aatccctaac ctccttatct gaagtggttc tacagaacag aa 1602 <210> 45 <211> 1604 <212> DNA <213> porcine endogenous retrovirus <400> 45 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtagagagct gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgct tggtttctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccagtac aggtattact attaacagca ctcaagggga ggctccctta 240 aggacctggt ggcctgagtt atatgtctgc cttcgatcgg taatccctgg tctcaacgac 300 caggccacac cccccgatgt actccgtgct tacaggtttt atgtttgccc aggaccccca 360 aataataaaa aatactgtga aaatcctcag gatttctttt acaaacaata gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccac ttataatcaa tttaattatg gccatgggag ataaaaagat 540 tgacaacagc gggtacaaaa agatgtacaa aataagcaaa taagctgtaa tttgttagac 600 ctagattact taaaaataag tttcactgaa aaaaaaaaca agaaaatatt caaaagtggg 660 taaatggtat gtcttgaaaa ataatgtact atggaggctc tgggaaaagg aaaggatcta 720 ttctaactat tcgcctcaga atacaaactc agatggaacc tccggttgct ataggaccaa 780 ataagggttt ggccgaacaa ggacctccaa tccaagaaca gaggccatct cctaacccct 840 ctgattacaa tacaacctct ggatcagtcc ccactgagcc taacatcact attaaaacag 900 gggcgaaact ttttagccta atccagggag cttttcaagc tcttaactcc acgactccag 960 aggctacctc ttcttgttgg ctttgcttag cttcgggccc accttactat gagaaaatgg 1020 ctagaggagg aaaattcaat gtgtcaaaga aacatagaga ccaatgtaca tagggatccc 1080 aaaataagct tacccttact ggggtttctg gaaaagacac ctgcataggg agggttcccc 1140 catcccacca acacctttgt aaccacactg aagcctttaa tcgaacctct aagagtcagt 1200 atctggtacc cggttatgac aggtggtgga catgtaatac tggattaccc cttgtgtttc 1260 caccctggtt ttcaaccaaa ctaaagactt ttgcgttatg gtccaaattg tcccccgggt 1320 gtactactat cccgaaaaaa cggtccttga taaatatgac tatagatata atcggccaaa 1380 aagagaaccc atatccctga cactagctgt aatgctcgga ctgggagtgg ctgcaggcgt 1440 gggaacagga acggctgccc taatcacagg accacaacag ctagaaaaag gaattagtga 1500 cctacatcga attgtaacaa aagatctcca agccctaaaa caatctgtca gtaacctaga 1560 ggaatcccta acctccttgt ctgaagtggt tctacagaac agga 1604 <210> 46 <211> 1607 <212> DNA <213> porcine endogenous retrovirus <400> 46 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatctcct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctcccttg 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt acgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaac tttttaacct catccaggga gcttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaaggca cctgcatagg gatggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag 1200 tatctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccttgg ttttcaacca aactaaagac ttttgcgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagagc ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc 1440 gtgggaacag gaacggctgc cctaatcaca ggaccgcaac agctggagaa aggacttagt 1500 aacctacatc gaatggtaac ggaagatctc caagccctag aaaaatctgt cagtaacctg 1560 gaggaatccc taacctcctt atctgaagtg gttctacaga acaggaa 1607 <210> 47 <211> 1597 <212> DNA <213> porcine endogenous retrovirus <400> 47 atgcatccca cgttaagctg gtgccacgtc ccgactcggg gtggagagcc gaaaagaccg 60 agaatcccct taagctttgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccctgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacggaac cccctgtggc agtgggaccc 720 gataaagtac tggctgaaca ggggcccccg gccctggagc caccgcataa cttgccggtg 780 ccccaattaa cctcgctgcg gcctgacata acacagccac ctagcaacgg taccactgga 840 ttgattccta ccaacacgcc tagaaactcc ccaggtgttc ctgttaagac aggacagaga 900 cccttcagtc tcatccaggg agctttccaa gccatcaact ccaccgaccc cgatgccact 960 tcttcttgtt ggctttgtct atcctcaggg cctccttatt atgaggggat ggctaaaaaa 1020 ggaaaattca atgtgaccaa agagcataga aatcaatgta catgggggtc ccgaaataag 1080 cttaccctca ctgaagtttc cgggaagggg acatgcatag gaaaagctcc cccatcccac 1140 caacaccttt gctatagtac tgtggtttat gagcaggcct cagaaaatca gtatttagta 1200 cctggttata acaggtggtg ggcatgcaat actgggttaa ccccctgtgt ttccacctca 1260 gtcttcaacc aatccaaaga tttctgtgtc atggtccaaa tcgtcccccg agtgtactac 1320 catcccgagg aagtggtcct tgatgaatat gactatcggt ataaccgacc gaaaagagaa 1380 cccgtatccc ttaccctagc tgtaatgctc ggattaggga cggccgttgg tgtaggaaca 1440 gggacagctg ccctgatcac aggaccatag cagctagaga aaggacttgg tgagctacat 1500 gcggccatga cagaagatct ccgagcctta gaggaatctg ttagcaacct agaagagtcc 1560 ctgacttctt tgtctgaagt ggttctacag aacagga 1597 <210> 48 <211> 1605 <212> DNA <213> porcine endogenous retrovirus <400> 48 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggagagcc gaaaaaactg 60 aaaatcccct taagcttcgc ctccatcgct tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgc ggacagcccg aacttccata aacccttatc tctcacctgg 180 ttacttactg actccagtac aggtattact attaacagca ctcaagggga ggctccctta 240 gggacctggt ggcctgagtt atatgtctgc cttcgatcgg taatccctgg tctcaacgac 300 caggccacac cccctgatgt actccgtgct tacgggttta atgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaagg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca gtcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtaa ttcgttagat 600 ctagattact taaaaataag ttttactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aataatgtac tatggaggca ctgggagaag gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggctgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgtttaca atacaacctc tggattagtc cccctgagcc taacttcact attaaaacag 900 gggcgaaact ttttagcctc atccagggag cttttcaagc tcttaactcc acgactccag 960 aggctacctc ttcttgttgg ctttgcttag ctttgggccc accttactat gagggaatgg 1020 ctagaggagg gaaattcaat gtgacaaagg aacatagaga ccaatgtaca tggggatccc 1080 aaaataagct tacccttact gaggtttctg gaaaaggcac ctgcataggg agggttcccc 1140 catcccacca acacctttgt aaccacactg aagcctttaa tcgaacctct gagagtcagt 1200 acctggtacc tggttatgac aggtggtggg catgtaatac tggattaacc ccttgtgttt 1260 ccaccctggt tttcaaccaa actaaagact tttgtgtttt ggtccaaatt gtcccccggg 1320 tgtactacta tcccgaaaaa gcagtccttg atgaatatga ctatagatat aatcggccaa 1380 aaagagaacc catatccctg acactagctg taatgctcgg attgggagtg gctgcaggcg 1440 tggaaacagg aacggctgcc ctaatcacag gaccacaaca gctggagaaa ggacttagtg 1500 acctacatcg aattgtaacg gaagatctcc aagccctaga aaaatctgtc agtaacctag 1560 aggaatccct aacctccttg tctgaagtgg ttctacagaa ccgga 1605 <210> 49 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 49 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga agctcccttg 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt acgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaac tttttaacct catccaggga gcttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaaggca cctgcatagg gatggttccc 1140 ccatcccacc aacacctttg taaccacact aaagccttta atcgaacctc tgagagtcag 1200 tatctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccttgg ttttcaacca aactaaagac ttttgcgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagagc ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc 1440 gtgggaacag gaacggctgc cctaatcaca ggaccgcaac agctggagaa aggacttagt 1500 aacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaaccta 1560 gaggaatccc taacctcctt gtctgaagtg gttctacaga accgga 1606 <210> 50 <211> 1597 <212> DNA <213> porcine endogenous retrovirus <400> 50 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ttccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacagaac cccctgtggc agtgggaccc 720 gataaagtac tggctgaaca ggggcccccg gccctggagc caccgcataa cttgccggtg 780 ccccaattaa cctcgctgcg gcctgacata acacagccgc ctagcaacag taccactgga 840 ttgattccta ccaacacgcc tagaaactcc ccaggtgttc ctgttaagac aggacagaga 900 ctcttcagtc tcatccaggg agctttccaa gccatcaact ccaccgaccc tgatgccact 960 tcttcttgtt ggctttgtct atcctcaggg cctccttatt atgaggggat ggctaaagaa 1020 ggaaaattca atgtgaccaa agagcataga aatcaatgta catgggggtc ccgaaataag 1080 cttaccctca ctgaagtttc cgggaagggg acatgcatag gaaaagctcc cccatcccac 1140 caacaccttt gctatagtac tgtggtttat gagcaggcct cagaaaatca gtatttagta 1200 cctggttata acaggtggtg ggcatgcaat actgggttaa ccccctgtgt ttccacctca 1260 gtcttcaacc aatccaaaga tttctgtgtc atggtccaaa tcgtcccccg agtgtactac 1320 catcctgagg aagtggtcct tgatgaatat gactatcggt ataaccgacc aaaaagagaa 1380 cccgtatccc ttaccctagc tgtaatgctc ggattaggga cggccgttgg cgtaggaaca 1440 gggacagctg ccctgatcac aggaccacag cagctagaga aaggacttgg tgagctacat 1500 gcggccatga cagaagatct ccgagcctta gaggagtctg ttagcaacct agaagagtcc 1560 ctgacttctt tatctgaagt ggttctacag aaccgaa 1597 <210> 51 <211> 1596 <212> DNA <213> porcine endogenous retrovirus <400> 51 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcattcctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca tcctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgtgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaagaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacagaac cccctgtggc agtgggaccc 720 gataaagtac tggctgaaca ggggccccgg ccctggagcc accgcataac ttgccggtgc 780 cccaattaac ctcgctgcgg cctgacataa cacagccgcc tagcaacggt accactggat 840 tgattcctac caacacgcct agaaactccc caggtgttcc tgttaagaca ggacagagac 900 tcttcagtct catccaggga gctttccaag ccatcaactc caccgaccct gatgccactt 960 cttcttgttg gctttgtcta tcctcagggc ctccttatta tgaggggatg gctaaagaag 1020 gaaaattcaa tgtgaccaaa gagcatagaa atcaatgtac atgggggtcc caaaataagc 1080 ttaccctcac tgaagtttcc gggaagggga catgcatagg aaaagctccc ccatcccacc 1140 aacacctttg ctatagtact gtggtttatg agcaggcctc agaaaatcag tatttagtac 1200 ctggttataa caggtggtgg gcatgcaata ctgggttaac cccctgtgtt tccacctcag 1260 tcttcaacca atccaaaaat ttctgtgtca tggtccaaat cgtcccccga gtgtactacc 1320 atcctgagaa agtggtcctt gatgaatatg actatcggta taaccgacca aaaagagaac 1380 ccgtatccct taccctagct gtaatgctcg gattagggac ggccgttggc gtaggaacag 1440 ggacagctgc cctgatcaca ggaccacagc agctagagaa aggacttggt gaactacatg 1500 cggccatgac agaagatctc cgagccttag aggagtctgt tagcaaccta gaagagtccc 1560 tgacttcttt atctgaagtg gttctacaga accgaa 1596 <210> 52 <211> 1555 <212> DNA <213> porcine endogenous retrovirus <400> 52 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagccca aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctcccttg 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt acgtttgccc aggaccccta 360 atgatgtaga tattgtgcaa tcctcagatt ctttgcagca tgagctgcgt actctatgat 420 ggattgaatg agagtctctc agcagacaga gtagtactct ttgtacatcc tacagtatat 480 catttattat ggcatggaga tggaagattg cacagcggta caaagatgta cgaatagcaa 540 taagctgtca ttcgttagac ctagatactt agaataagtt cactgacaag aaacaagata 600 atattcaaaa gtgggtaaat ggtatgtctt gggaatagtg tactatggag gctctgggag 660 aaagaaagga tctgttctga ctattcgcct cagaatagaa actcagatgg aacctccgtg 720 ctatagacca aataagagtt tggccgaaca aggacctcca atccaagaac agaggccatc 780 tcctaacccc tctgattaca atacaacctc tggatcagtc cccactgaag ctaacatcac 840 tattaaaaca ggggcgaaac tttttaactc atccagggag ctttccaagc tcttaactcc 900 acgactccag aggctacctc ttcttgttgg ctttgcttag cttcgggccc accttactat 960 gagggaatgg ctagaggagg gaaattcaat gtgacaaagg aacatagaga ccaatgtaca 1020 tggggaaccc aaaataagct tacccttact gaggtttctg gaaaaggcac ctgcataggg 1080 atggttcccc catcccacca acacctttgt aaccacacta aagcctttaa tcgaacctct 1140 gagagtcagt atctggtacc tggttatgac aggtggtggg catgtaatac tggattaacc 1200 ccttgtgttt ccaccttggt tttcaaccaa actaaagact tttgcgttat ggtccaaatt 1260 gtcccccggg tgtactacta tcccgaaaaa gcagtccttg atgaatatga ctatagatat 1320 aatcggccaa aaagagagcc catatccctg acactagctg taatgctcgg attgggagtg 1380 gctgcaggcg tgggaacagg aacggctgcc ctaatcacag gaccgcaaca gctggagaaa 1440 ggacttagta acctacatcg aattgtaacg gaagatctcc aagccctaga aaaatctgtc 1500 agtaacctag aggaatccct aacctccttg tctgaagtgg ttctacagaa ccgga 1555 <210> 53 <211> 1591 <212> DNA <213> porcine endogenous retrovirus <400> 53 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctca aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatgag ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat agagctgcgt cacccccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact acaaaggaaa acagaaaata ttcaaaagtg 600 ataaatggta tgagctggga atagtttttt ataaatatgg cgggggagca gggtccactt 660 taaccattcg ccttaggata gagacgggac agaaccccct gtggcagtgg gacccgataa 720 agtactggct gaacaggggc cccggccctg gagccaccgc ataacttgcc ggtgccccaa 780 ttaacctcgc tgcggcctga cataacacag ccgcctagca acggtaccac tggattgatt 840 cctaccaaca cgcctagaaa ctccccaggt gttcctgtta agacaggaca gagactcttc 900 agtctcatcc agggagcttt ccaagccatc aactccaccg accctgatgc cacttcttct 960 tgttggcttt gtctatcctc agggcctcct tattatgagg ggatggctaa agaagaaaaa 1020 ttcaatgtga ccaaagaaca tagaaatcaa tgtacatggg ggtctcgaaa taagcttacc 1080 ctcactgaag tttccgagaa ggggacatgc ataggaaaag ctcccccatc ccaccaacac 1140 ctttgctata gtactgtggt ttatgagcag gcctcagaaa atcagtattt agtacctggt 1200 tataacaggt ggtgggcata caatactggg ttaaccccct gtgtttccac ctcagtcttc 1260 aaccaatcca aagatttctg tgtcatggtc caaatcgtcc cccgagtgta ctaccatcct 1320 gagaaagtgg tccttgatga atatgactat cggtataacc gaccaaaaag agaacccgta 1380 tcccttaccc tagctgtaat gctcggatta gggacggccg ttggcgtagg aacagggaca 1440 gctgccctga tcacaggacc acagcagcta gagaaaggac ttggtgagct acatgcggcc 1500 atgacagaag atctccgagc cttagaggag tctgttagca acctagaaga gtccctgact 1560 tctttgtctg aagtggttct acagaacagg a 1591 <210> 54 <211> 1597 <212> DNA <213> porcine endogenous retrovirus <400> 54 atgcatccca cgttaagctg gcgccacgtc ccgacttggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacaccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccaattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg atccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggag aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacggaac cccctgtggc agtgggaccc 720 gataaagtac tggctgaaca ggggccccca gccctggagc caccgcataa cttgccggtg 780 ccccaattaa cctcgctgcg gcctgacata acacagccgc ctagcaacgg taccactgga 840 ttgattccta ccaacacgcc tagaaactcc ccaggtgttc ctgttaagac aggacagaga 900 cccttcagtc tcatccaggg agctttccaa gccatcaact ccaccgatcc cgatgccact 960 tcttcttgtt ggctttgtct atcctcaggg cctccttatt atgaggggat ggctaaaaaa 1020 ggaaaattca atgtgaccaa agagcataga aatcaatgta catgggggtc ccgaaataag 1080 cttaccctca ctgaagtttc cgggaagggg acatgcatag gaaaagctcc cccatcccac 1140 caacaccttt gctatagtac tgtggtttat gagcaggcct cagaaaatca gtatttagta 1200 cctggttata acaggtggtg ggcatgcaat actgggttaa ccccctgtgt ttccacctca 1260 gtcttcaacc aatccaaaga tttctgtgtc atggtccaaa tcgtcccccg agtgtactac 1320 catcccgagg aagtggtcct tgatgaatat gactatcggt ataaccgacc gaaaagagaa 1380 cctgtatccc ttaccctagc tgtaatgctc ggattaggga cggctgttgg cgtaggaaca 1440 gggatagctg ccctgatcac aggaccacag cagctagaga aaggacttgg tgagctacat 1500 gaggccatga cagaagatct ccgagcctta gaggagtctg ttagcaacct agaagatccc 1560 ctgacttctt tgtctgaagt ggttctacag aacagga 1597 <210> 55 <211> 1595 <212> DNA <213> porcine endogenous retrovirus <400> 55 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgag ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacagaac cccctctggc agtgggaccc 720 gataaagtac tggctgaaca ggggccccgg ccctggagcc accgcataac ttgccggtgc 780 cccaattaac ctcgctgcgg cctgacataa cacagccgcc tagcaacggt accactggat 840 tgattcctac caacacgcct agaaactccc caggtgttcc tgttaagaca ggacagagac 900 tcttcagtct catccaggga gctttccaag ccatcaactc cactgaccct gatgccactt 960 cttcttgttg gctttgtcta tcctcagggc ctccttatta tgaggggatg gctaaagaag 1020 gaaaattcaa tgtgaccaag agcatagaaa tcaatgtaca tgggggtccc gaaataagct 1080 taccctcact gaagtttccg ggaaggggac atgcatagga aaagctcccc catcccacca 1140 acacctttgc tatagtactg tggtttatga gcaggcctca gaaaatcagt atttagtacc 1200 tggttataac aggtggtggg catgcaatac tgggttaacc ccctgtgttt ccacctcagt 1260 cttcaaccaa tccaaagatt tctgtgtcat ggtccaaatc gtcccccgag tgtactacca 1320 tcctgaggaa gtggtccttg atgaatatga ctatcggtat aaccgaccaa aaagagaacc 1380 cgtatccctt accctagctg taatgctcgg attagggacg gccgttggcg taggaacagg 1440 gacagctgcc ctgatcacag gaccacagca gctaaagaaa ggacttggtg agctacatgc 1500 ggccatgaca gaagatctcc gagccttaga ggagtctgtt agcaacctag aagagtccct 1560 gacttctttg tctgaagtgg ttctacagaa cagga 1595 <210> 56 <211> 1595 <212> DNA <213> porcine endogenous retrovirus <400> 56 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaaggctg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga gactcccttg 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt acgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggaaaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataaggttt ggccgaacaa ggacctccat ccaagaacag aggcatctcc taacccctct 840 gattacaata caacctctgg atcagtcccc actgagccta acatcactat taaaacaggg 900 cgaaactttt agctcatcca gggagctttt caagctctta actcccgact ccagaggcta 960 cctcttcttg ttggctttgc ttagcttcgg cccccttact atgagggaat ggctagagga 1020 gggaaattca atgtgccaaa ggaacataga gaccaatgta catggggatc ccaaaataag 1080 cttaccctta ctgaggtttc tggaaaaggc acctgcatag ggatggttcc cccatcccac 1140 caacacattt gtaaccacac tgaagccttt aatcgaacct ctgagagtca gtatctggta 1200 cctggttatg acaggtggtg ggcatgtaat actggattaa ccccttgtgt ttccaccttg 1260 gttttcaacc aaactaaaga cttttgcgtt atggtccaaa ttgtcccccg ggtgtactac 1320 tatcccgaaa aagcagtcct tgatgaatat gactatagat ataatcggcc aaaaagagag 1380 cccatatccc tgacactagc tgtaatgctc ggattgggag tggctgcagg cgtgggaaca 1440 ggaacggctg ccctaatcac aggaccgcaa cagctggaga aaggacttag tgacactaca 1500 tcgaattgta acggaagatc tccaagccct agaaaatctg tcagtagcat ggagatccct 1560 tagctcctta tctgaagtgg ttctacagaa cagaa 1595 <210> 57 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 57 atgcatccca cgttaagcca gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aatgccttgt ggacagcctg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctccctta 240 gggacctggt ggcatgagtt atatgtctgc cttcgatcag taatccctgg cctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt atgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgcaac tttttagcct catccaggga gcttttcaag cccttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaagtttct ggaaaaggca cctgcatagg gagggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag 1200 tatctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccttgg ttttcaacca aactaaagac ttttgcttta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagagc ccatatccct gacactagct gtaatgcttg gattgggagt ggctgcaggc 1440 gtgggaacag gaacggctgc cctaatcaca ggaccacaac agctggagaa aggacttagt 1500 aacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaacctg 1560 gaggaatccc taacctcctt gtctgaagtg gttctacaga accgaa 1606 <210> 58 <211> 1604 <212> DNA <213> porcine endogenous retrovirus <400> 58 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggagagcc gaaaaaactg 60 aaaatcccct taagcttcgc ctccatcgct tggttcccta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgc ggacagcccg aacttccata aacccttatc tctcacctgg 180 ttacttactg actccagtac aggtattact attaacagca ctcaagggga ggctccctta 240 gggacctggt ggcctgagtt atatgtctgc cttcgatcgg taatccctgg tctcaacgac 300 caggccacac cccctgatgt actccgtgct tacgggttta atgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaaga gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca gtcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtaa ttcgttagat 600 ctagattact taaaaataag ttttcactga aaaaggaaaa caagaaaata ttcaaaagtg 660 ggtaaatggt atgtcttggg gaataatgta ctatggaggc actgggagaa ggaaaggatc 720 tgttctgact attcgcctca gaatagaaac tcagatggaa cctccggttg ctataggacc 780 aaataagggt ttggctgaac aaggacctcc aatccaagaa cagaggccat ctcctaaccc 840 ctctgtttac aatacaacct ctggattagt ccccctgagc ctaacttcac tattaaaaca 900 ggggcgaaac tttttagcct catccaggga gcttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gctttgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaaggca cctgcatagg gagggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag 1200 tacctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccctgg ttttcaacca aactaaagac ttttgtgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagagc ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc 1440 gtggaaacag gaacggctgc ccataatcac aggaccacaa cagctggaga aaggacttag 1500 tgtcaataga tcgaatgtaa cggaagatct ccaagcccta gaaaaatcgt cagtagcgag 1560 agatgctagc actggcttat ctgaagtggt tctacagaac agaa 1604 <210> 59 <211> 1591 <212> DNA <213> porcine endogenous retrovirus <400> 59 atgcatccca cgttaaggca gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aatgccttgt ggacagcctg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctccctta 240 gggacctggt ggcatgagtt atatgtctgc cttcgatcag taatccctgg cctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt atgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttacat cctaccagtt atatcattta attatggcca tggagatgga aagattggca 540 cagcgggtac aaaaagatgt acgaataagc aaataagctg tcattcgtta gacctagatt 600 acttaaaaat aagttcactg aaaagcaaac aagaaatatt caaagtgggt aaatggtatg 660 tcttgggaat agtgtactat ggaggctctg ggagaaagaa aggatctgtt ctgactattc 720 gcctcagata gaaactcaga tggaacctcc ggttgctata ggaccaaata agggtttgcc 780 gaacaaggac ctccaatcca agaacagagg ccatctccta acccctctga ctacaataca 840 acctctggat cagtccccac tgagcctaac atcactatta aaacaggggc gaaacttttt 900 agcctcatcc agggagcttt tcaagccctt aactccacga ctccagaggc tacctcttct 960 tgttggcttt gcttagcttc gggcccacct tactatgagg gaatggctag aggagggaaa 1020 ttcaatgtga caaaggaaca tagagaccaa tgtacatggg gatcccaaaa taagcttacc 1080 cttactgagg tttctggaaa aggcacctgc atagggaggg ttcccccatc ccaccaacac 1140 ctttgtaacc acactgaagc ctttaatcga acctctgaga gtcagtatct ggtacctggt 1200 tatgacaggt ggtgggcatg taatactgga ttaacccctt gtgtttccac cttggttttc 1260 aaccaaacta aagacttttg ctttatggtc caaattgtcc cccgggtgta ctactatccc 1320 gaaaaagcag tccttgatga atatgactat agatataatc ggccaaaaag agagcccata 1380 tccctgacac tagctgtaat gcttggattg ggagaggctg caggcgtggg aacaggaacg 1440 gctgccctaa tcacaggacc acaacagctg gagaaaggac ttagtaacct acatcgaatt 1500 gtaacggaag atctccaagc cctagaaaaa tctgtcagta acctggagga atccctaacc 1560 tccttgtctg aagtggttct acagaacagg a 1591 <210> 60 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 60 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctcccttg 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccctgatgt actccgtgct tacgggtttt acgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aataaaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaac tttttaacct catccaggga gcttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaaggca cctgcatagg gatggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag 1200 tatctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccttgg ttttcaacca aactaaagac ttttgcgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagagc ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc 1440 gtgggaacag gaacggctgc cctaatcgca ggaccgcaac agctggagaa aggacttagt 1500 aacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaacctg 1560 gaggaatccc taacctcctt atctgaagtg gttctacaga acagga 1606 <210> 61 <211> 1592 <212> DNA <213> porcine endogenous retrovirus <400> 61 atgcatccca cgttaagccg gcgctacctc ccgattcggg gtggagagcc gaaaagactg 60 aaaaccccct taagcttcgc ctccatcgct tggttcctta atctgtaaat aactcctcaa 120 gttaatggta aacgcccgaa ctcccacaaa cccttatctc tcacctggtt acttactgac 180 tccagtacag gtattactat taacagcact caaggggagg ctcccttagg gacctggtgg 240 cctgagttat atgtctgcct tcgatcggta atccctggtc tcaacgacca ggccacaccc 300 cccgatgtac tccgtgctta tgggttttat gtttgcccag gacccccaaa taatgaagaa 360 tattgtggaa atcctcagga tttcttttgc aagcaatgga gctgcgtaac ttctaatgat 420 gggaattgga aatggccagt ctctcagcaa gacagagtaa gttactcttt tgttaacaat 480 cctaccagtt ataatcaatt taattaccgc catgggaaat ggaaagattg gcaacagcag 540 gtacaaaaag atgtacgaag taagcaaata agctgtaatt tgttagacct agattactta 600 aaaataagtt tcactgaaaa aggaaaacaa gaaaatattc aaaagtgggt aaatggtatg 660 tcttggggaa tagtgtacta tggaggctct gggagaagga aaggatctat tctaactatt 720 cgcctcagaa tagaaactca gatggaacct ccggttgcta taggaccaaa taagtgtttg 780 gccgaacaag gacctccaat ccaagaacag aggccatctc ttaaccctct gattacatac 840 agcctctgga tcagtcccca ctgagcctaa catcactatt aaaacagggg caaaactttt 900 tagcctcatc cagggagctt ttcaagctct taactccaca actccagagg ctacctcttc 960 ttgttggctt tgcttagctt caggccccac cttactatga ggaacggcta gaggagggaa 1020 attcaatgtg acaaaggaac atagagacca atgtacatgg ggatcccaaa ataagcttac 1080 ccttactgag gtttctggaa aaggcacctg catagggagg gttcccccat cccaccaaca 1140 cctttgtaac cacactgaag cctttaatcg aacctctgag agtcagtatc tggtacccgg 1200 ttatgacagg tggtgggcat gtaatactgg attaacccct tgtgtttcca ccctggtttt 1260 caaccaaact aaagactttt gcgttatggt ccaaattgtc ccccgggtgt actactatcc 1320 cgaaaaagcg gtccttgatg aatatgacta tagatataat cagccaaaaa gagaacccat 1380 atccctgaca ctagctgtaa tgctcggatt gggagtggct gcagccgtgg gaacaggaac 1440 ggctgcccta atcacaggac cacaacagct ggagaaagga cttagtgacc tacatcgaat 1500 tgtaacggaa gatctccaag ccctagaaaa atctgtcagt aacctagagg aatccctaac 1560 ctccttatct gaagtggttc tacagaacag aa 1592 <210> 62 <211> 1578 <212> DNA <213> porcine endogenous retrovirus <400> 62 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctaaaacgac tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gccgaggcgc agagaaagag 360 acatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaagt 600 ggataaatgg tatgagctgg ggaatagttt tttataaata tggcggggga gcagggtcca 660 ctttaaccat ttgccttagg atagagacgg gacagaaccc ctgtggcagt gggacccgat 720 aaagtactgg ctgaacaggg gcccccggcc ctggagccac cgcataactt gccggtgccc 780 cattaacctc gctgcggcct gacataacac agccgcctag caacggtacc actggattga 840 ttcctaccaa cacgcctaga actccccagt gttcctgtta agacaggaca gaaactcttc 900 agtctcatca ggagctttcc agccatcact cacggacctg atgcactctt cttgttggct 960 ttgtctatcc tcagggcctc cttattatga gggatggcta aagaaggaaa attcaatgtg 1020 accaagagca tagaaatcaa tgtacatggg gtcccgaaat aagcttaccc tcactgaagt 1080 ttccgggaag gggacatgca taggaaaagc tcccccatcc caccaacacc tttgctatag 1140 tactgtggtt tatgagcagg cctcagaaaa tcagtattta gtacctggtt ataacaggtg 1200 gtgggcatgc aatactgggt taaccccctg tgtttccacc tcagtcttca accaatccaa 1260 agatttctgt gtcatggtcc aaatcgtccc ccgagtgtac taccatcctg aggaagtggt 1320 ccttgatgaa tatgactatc ggtataaccg accaaaaaga gaacccgtat cccttaccct 1380 agctgtaatg ctcggattag ggacggccgt tggcgtagga acagggacag ctgccctgat 1440 cacaggacca cagcagctag agaaaggact tggttaacta catgcggcca tgacagaaga 1500 tctccgagcc ttagaggagt ctgtagcacc tagaagagta catgactgtc cagtctgaag 1560 tggttctaca gaaccgga 1578 <210> 63 <211> 1602 <212> DNA <213> porcine endogenous retrovirus <400> 63 atgcatccca cgttaaggcg gcgccacctc ccgattcggg gtagagatct gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgct tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccagtac aggtattact attaacagca ctcaagggga ggctccctta 240 aggacctggt ggcctgagtt atatgtctgc cttggatcgg taatccctgg tctcaacgac 300 caggccacac cccccgatgt actccatgct tacaggtttt atgtttgccc aggaccccca 360 aataataaaa aatactgtga aaatcctcag gatttctttt acaaacaata gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcaac aagacagagt aagttactct 480 tttgttaaca atcctaccac ttataatcaa tttaattatg gccatgggag ataaaaagat 540 tgacaacagc gggtacaaaa agatgtacaa aataagcaaa taagctgtat tttgttagac 600 ctagattact taaaaataag tttcactgaa aaagcaaaac aagaaatatt ccaaagtggg 660 taaatggtat gtcttgaaga ataatgtact atggaggctc tgggaaaaag gaaaggatct 720 attctgactt ttcgcctcag aatacaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctacatcact attaaaacag 900 gggcgaaact ctttagccta atccagggaa cttttcaagc tctaactcca cgactccaga 960 ggctacctct tcttgttggc tttgcttagc ttcgggccca ccttactatg agacaatggc 1020 tagaggagga aaattcaatg tgacaaagaa acatagagac caatgtacat agggatccca 1080 aaataagctt acccttactg aggtttctgg aaaagacacc tgcataggga gggttccccc 1140 atcccaccaa cacctttgta accacactga agcctttaat cgacctctaa gagtcagtat 1200 ctggtacccg gttatgacag gtggtggaca tgtaatactg gattacccct tgtgtttcca 1260 ccctggtttt caaccaaact aaagactttt gcgttatggt ccaaattgtc ccccgggtgt 1320 actactatcc cgaaaaaacg gtccttgata aatatgacta tagatataat tggccaaaaa 1380 gagaacccat atccctgaca ctagctgtaa tgctcggact gggagtggct gcaggcgtgg 1440 gaacaggaac ggctgcccta atcacaggac cacaacagct agaaaaagga attagtgacc 1500 tacatcgaat tgtaacaaaa gatctccaag ccctaaaaca atctgtcagt aacctagagg 1560 aatccctaac ctccttatct gaagtggttc tacagaaccg ga 1602 <210> 64 <211> 1598 <212> DNA <213> porcine endogenous retrovirus <400> 64 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacagaac cccctgtggc agtgggaccc 720 gataaagtac tggctgaaca ggggcccccg gccctggggc caccgcataa cttgccggtg 780 ccccaattaa cctcgctgcg gcctgacata acacagccgc ctagcaacgg taccactgga 840 ttgattccta ccaacacgcc tagaaactcc ccaggtgttc ctgttaagac aggacagaga 900 ctcttcagtc tcatccaggg agctttccaa gccatcaact ccaccgaccc tgatgccact 960 tcttcttggt ggctttggct atcctcaggg cctccttatt atggagggga tggctaaaga 1020 aggaaaattc aatgtgacca aagagcatag aaatcaatgt acatgggggt cccgaaataa 1080 gcttaccctc actgaagttt ccgggaaggg gacatgcata ggaaaagctc ccccatcaca 1140 ccaacacctt tgctatagta ctgtggttta tgagcaggcc tcagaaaatc agtatttagt 1200 acctggttat aacaggtggt gggcatgcaa tactgggtta accccctgtg tttccacctc 1260 agtcttcaac caatccaaag atttctgtgt catggtccaa atcgtccccc gagtgtacta 1320 ccatcctgag gaagtggtcc ttgatgaata tgactatcgg tataaccgac caaaaagaga 1380 acccgtatcc cttaccctag ctgtaatgct cggattaggg acggccgttg gcgtaggaac 1440 agggacagct gccctgatca caggaccaca gcagctagag aaaggacttg gtgagctaca 1500 tgcggccatg acagaagatc tccgagcctt agaggagtct gttagcaacc tagaagagtc 1560 cctgacttct ttgtctgaag tggttctaca gaacagga 1598 <210> 65 <211> 1596 <212> DNA <213> porcine endogenous retrovirus <400> 65 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctcccttg 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt acgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaac tttttagcct catccaggga gcttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgaggaatgg 1020 ctagaggagg gaaattcatg tgacaaggaa catagagacc aatgtacatg ggatcccaaa 1080 atagcttacc atactgaggt tctggaatgg cactgcatag ggatggttcc cccatcccac 1140 caacaccttg taaccacact gaagccttta atcgaacctc tgagagtcag tatctggtac 1200 ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt tccaccttgg 1260 ttttcaacca aactaaagac ttttgcgtta tggaccaaat tgtcccccgg gtgtactact 1320 atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca aaaagagagc 1380 ccatattcct gacactagct gtaatgctcg gattgggagt ggctgcaggc gtgggaacag 1440 gaacggctgc cctaatcaca ggaccgcaac agctggagaa aggacttagt aacctacatc 1500 gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaacctg gaggaatccc 1560 taacctcctt atctgaagtg gttctacaga acagaa 1596 <210> 66 <211> 1603 <212> DNA <213> porcine endogenous retrovirus <400> 66 atgcatccca cgttaagggc ggtgccacct cccgattcgg ggtggaaagc cgaaaagact 60 gaaaattccc ttaagcttcg cctccatcgc gtggttcctt actctgtcaa taactcctca 120 agttaatggt aaacgccttg tggacagccc gaactcccat aaacccttat ctctcacctg 180 gttacttact gactccggta caggtattaa tattaacagc actcaagggg aggctccctt 240 agggacctgg tggcctgagg tatatgtctg ccttcgatca gtaatccctg gtctcagtga 300 ccaggccaca ccccccgatg tactccgtgc ttacgggttt tatgtttgcc caggaccccc 360 aaataatgaa gaatattgtg gaaatcctca ggatttcttt tgcaagcaat ggagctgcgt 420 aacttctaat gatgggaatt ggaaatggcc agtctctcag caagacagag taagttactc 480 ttttgttaac aatcctacca gttataatca atttaattat ggccatggga gatggaaaga 540 ttggcaacag cgggtacaaa aagatgtacg aaataagcaa ataagctgtc attcgttaga 600 cctagattac ttaaaaataa gtttcactga aaaaggaaaa caagaaaata tgcaaaagtg 660 ggtaaatggt atgttttggg gaatagtgta ctatggaggc tctgggagaa agaaaggatc 720 tgttctgact attcgcctca gaatagaaac tcagatggaa cctccggttg ctataggacc 780 aaataagggt ttggccgaac aaggacctcc aatccaagaa cagaggccat ctcctaaccc 840 ctctgattac aatacaacct ctggatcagt ccccactgag cctaacatca ctattaaaac 900 aggggcgaaa ctttttagcc tcatccaggg agcttttcaa gctcttaact ccacgactcc 960 agaggctacc tcttcttgtt ggctttgctt agcttcgggc ccaccttact atgagggaat 1020 ggctagagga gggaaattca atgtgacaaa ggaacataga gaccaatgta catggggatc 1080 ccaaaataag cttaccctta ctgaggtttc tggaaaaggc acctgcatag ggagggttcc 1140 cccatcccac caacaccttt gtaaccacac tgaagccttt aatcgaacct ctgagagtca 1200 gtatctggta cctggttatg acaggtggtg ggcatgtaat actggattaa ccccttgtgt 1260 ttccaccttg gttttcaacc aaactaaaga cttttgcgtt atggtccaaa ttgtcccccg 1320 ggtgtactac tatcccgaaa aagcagtcct tgatgaatat gactatagat ataatcggcc 1380 aaaatgagag cccatatccc tgacactagc tgtaatgctc ggattgggag tggctgcagg 1440 cgtgggaaca ggaacggctg cccataatca caggaccaca acagctggag aaaggactta 1500 gtaacctacc tcgaattgta acggaagatc tccaagccct gaaaaatctg tcagtagcct 1560 gagatactac tctccgaatc tgaagtggtt ctacagaaca gaa 1603 <210> 67 <211> 1590 <212> DNA <213> porcine endogenous retrovirus <400> 67 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggagagcc gaaaagactg 60 aaaaccccct taagcttcgc ctccatcgct tggttcctta ctctgtaaat aactcctcaa 120 gttaatggta aacgcccgaa ctcccacaaa cccttatctc tcacctggtt acttactgac 180 tccagtacag gtattactat taacagcact caaggggagg ctcccttagg gacctggtgg 240 cctgagttat atgtctgcct tcgatcggta atccctggtc tcaacgacca ggccacaccc 300 cccgatgtac tccgtgctta tgggttttat gtttgcccag gacccccaaa taatgaagaa 360 tattgtggaa atcctcagga tttcttttgc aagcaatgga gctgcgtaac ctctaatgat 420 gggaattgga aatggccagt ctctcagcaa gacagagtaa gttactcttt tgttaacaat 480 cctaccagtt ataatcaatt taattaccgc catgggaaat ggaaagattg gcaacagcag 540 gtacaaaaag atgtacgaaa taagcaaata agctgtaatt tgttagacct agattactta 600 aaaataagtt tcactgaaaa aggaaaacaa gaaaatattc aaaagtgggt aaatggtatg 660 tcttggggaa tagtgtacta tggaggctct gggagaagga aaggatctat tctaactatt 720 cgcctcagaa tagaaactca gatggaacct ccggttgcta taggaccaaa taagtgttgg 780 gccgaacaag gacctccaat ccaaggacag aggccatctc ttaacccctc tgattacaat 840 acagcctttg gatcagtccc cactgagcct aacatcacta ttaaaacagg ggcaaaactt 900 ttagcctcat ccagggagct tttcaagctc ttaactccac aactccagag gctacctctt 960 cttgttggct ttgcttagct tcaggcccac cttactatga ggaacggcta gaggagggaa 1020 attcaatgtg acaaaggaac atagagacca atgtacatgg ggatcccaaa ataagcttac 1080 ccttactgag gtttctggaa aaggcacctg catagggagg gttcccccat cccaccaaca 1140 cctttgtaac cacactgaag cctttaatcg aacctctgag agtcagtatc tggtacccgg 1200 ttatgacagg tggtgggcat gtaatactgg attaacccct tgtgtttcca ccctggtttt 1260 caaccaaact aaagactttt gcgttatggt ccaaattgtc ccccgggtgt actactatcc 1320 cgaaaaagcg gtccttgatg aatatgacta tagatataat cagccaaaaa gaagcccata 1380 tccctgacac tagctgtaat gctcggattg ggagtggctg caggcgtggg aacaggaacg 1440 gctgccctaa tcacaggacc acaacagctg gagaaaggac ttagtgacct acatcgaatt 1500 gtaacggaag atctccaagc cctagaaaaa tctgtcagta acgtagagat ccgtagcact 1560 gcttatctga agtggttcta cagaacagaa 1590 <210> 68 <211> 1605 <212> DNA <213> porcine endogenous retrovirus <400> 68 atgcatccca cgttaaggcg gcgccacctc ccgattcggg gtggagagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgct tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgc ggacagcccg aacttccata aacccttatc tctcacctgg 180 ttacttactg actccagtac aggtattact attaacagca ctcaagggga ggctccctta 240 gggacctggt ggcctgagtt atatgtctgc cttcgatcgg taatccctgg tctcaacgac 300 caggccacac cccctgatgt actctgtgct tacaggttta atgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaagg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca gtcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtaa ttcgttagat 600 ctagattact taaaaataag ttttactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aataatgtac tatggaggca ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggctgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgtttaca atacaacctc tggattagtc cccctgagcc taacttcact attaaaacag 900 gggcgaaact ttttagcctc atccagggag cttttcaagc tcttaactcc acgactccag 960 aggctacctc ttcttgttgg ctttgcttag ctttgagccc accttactat gagggaatgg 1020 ctagaggagg gaaattcaat gtgacaaagg aacatagaga ccaatgtaca tggggatccc 1080 aaaataagct tacccttact gaggtttctg gaaaaggcac ctgcataggg agggtttccc 1140 catcccacca acacctttgt aaccacactg aagcctttaa tcgaacctct gagagtcagt 1200 acctggtacc tggttatgac aggtggtggg catgtaatac tggattaacc ccttgtgttt 1260 ccaccctggt tttcaaccaa actaaagact tttgtgttat ggtccaaatt gtcccccggg 1320 tgtactacta tcccgaaaaa gcggtccttg atgaatatga ctatagatat aatcggccaa 1380 aaagagaacc catatccctg acactagctg taatgctcgg attgggagtg gctgcaggcg 1440 tgggaacagg aacggctgcc ctaatcacag gaccacaaca gctggagaaa ggacttagtg 1500 acctacatcg aattgtaacg gaagatctcc aagccctaga aaaatctgtc agtaacctag 1560 aggaatccct aacctccttg tctgaagtgg ttctacagaa cagga 1605 <210> 69 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 69 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctcccttg 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt acgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaac tttttagcct catccaggga gcttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaagggca cctgcatagg gatggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag 1200 tatctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccttgg ttttcaacca aactaaagac ttttgcgtta tggaccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagagc ccatattcct gacactagct gtaatgctcg gattgggagt ggctgcaggc 1440 gtgggaacag gaacggctgc cctaatcaca ggaccgcaac agctggagaa aggacttagt 1500 aacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaacctg 1560 gaggaatccc taacctcctt atctgaagtg gttctacaga acagaa 1606 <210> 70 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 70 atgcatccca cgttaagccg gagccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttccttc ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctcccttg 240 gggacctggt ggcctgaatt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt acgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gcctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaac tttttagcct catccaggga gcttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaaggca cctgcatagg gatggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag 1200 tatctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccttgg ttttcaacca aactaaagac ttttgcgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagagc ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc 1440 gtgggaacag gaacggctgc cctaatcaca ggaccgcaac agctggagaa aggacttagt 1500 aacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaacctg 1560 gaggaatccc taacctcctt atctgaagtg gttctacaga accgaa 1606 <210> 71 <211> 1595 <212> DNA <213> porcine endogenous retrovirus <400> 71 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ccctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagagagaa 360 atactgtggg ggttccgggg aatccttctg taggagatgg agctgcgtca cctccaacga 420 tggagactgg aaatggccga tctctctcca ggaccgggta aaattctcct ttgtcaattc 480 cggcccgggc aagtacaaag tgatgaaact atataaagat aagagctgct ccccatcaga 540 cttagattat ctaaagataa gtttcactga aaaaggaaaa cgggaaaata ttcaaaagtg 600 gataaatggt atgagctggg gaatagtttt ttataaatat ggcgggggag cagggtccac 660 tttaaccatt cgccttagga tagagacggg gacagaaccc cctgtggcag tgggacccga 720 taaagtactg gctgaacagg ggcccccggc cctggagcca ccgcataact tgccggtgcc 780 ccaattaacc tcgctgtggc ctgacataac acagccgcct agcaacggta ccactggatt 840 gattcctacc aacatgccta gaaactcccc aggtgttcct gttaagacag gacagagact 900 cttcagtctc atccagggag ctttccaagc catcaactcc accgaccctg atgccacttc 960 ttcttgttgg ctttgtctat cctcagggcc tccttattat gaggggatgg ctaaagaagg 1020 aaaattcaat gtgaccaaag agcatagaaa tcaatgtaca tgggggtccc gaaataagct 1080 taccctcact gaagtttccg ggaaggggac atgcatagga aaagctcccc catcccatca 1140 acacctttgc tatagtactg tggtttatga gcaggcctca gaaaatcagt atttagtacc 1200 tggttataac aggtggtggg catgcaatac tgggttaacc ccctgtgttt ccacctcagt 1260 cttcaaccaa tccaaagatt tctgtgtcat ggtccaaatc gtcccccgag tgtactacca 1320 tcctgaggaa gtggtccttg atgaatatga ctatcggtat aaccgaccaa aaagagaacc 1380 cgtatccctt accctagctg taatgctcgg attagggacg gccgttggcg taggaacagg 1440 gacagctgcc ctgatcacag gaccacagca gctagagaaa ggacttggtg agctacatgc 1500 ggccatgaca gaagatctcc gagccttaga ggagtctgtt agcaacctag aagagtccct 1560 gacttctttg tctgaagtgg ttctacagaa ccgaa 1595 <210> 72 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 72 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aatgccttgt ggacagcctg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctccctta 240 gggacctggt ggcatgagtt atatgtctgc cttcgatcag taatccctgg cctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt atgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaac tttttagcct catccaggga gcttttcaag cccttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacgtagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaaggca cctgcatagg gagggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag 1200 tatctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccttgg ttttcaacca aactaaagac ttttgcttta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagagc ccatatccct gacactagct gtaatgcttg gattgggagt ggctgcaggc 1440 gtgggaacag gaacggctgc cctaatcaca ggaccacaac agctggagaa aggacttagt 1500 aacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaacctg 1560 gaggaatccc taacctcctt gtctgaagtg gttctacaga acagaa 1606 <210> 73 <211> 1593 <212> DNA <213> porcine endogenous retrovirus <400> 73 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcggc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcgc agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca tttgccttag gatagagacg gggacagaac cccctgtggc agtgggaccc 720 gataaagtac tggctgaaca ggggcccccg gccctggagc caccgcataa cttgccggtg 780 ccccaataaa cttcgctgcg gctgacataa cacagccgcc tagcaacggt accactgatt 840 gattcctaca aacacgccta gaaactcccc aagtgttcct gttaagacag acagagactc 900 ttcagtctca tccagggagc tttccaagcc atcaactcca ccgaccctga tgccacttct 960 tcttgttggc tttgtctatc ctcagggcct ccttattatg aggggatggc taaagaagga 1020 aaattcaatg tgaccaagag catagaaatc aatgtacatg ggggtcccga aataagctta 1080 ccctcactga agtttccggg aaggggacat gcataggaaa agctccccca tcccaccaac 1140 acctttgcta tagtactgtg gtttatgagc aggcctcaga aaatcagtat ttagtacctg 1200 gttataacag gtggtgggca tgcaatactg ggttaacccc ctgtgtttcc acctcagtct 1260 tcaaccaatc caaagatttc tgtgtcatgg tccaaatcgt cccccgagtg tactaccatc 1320 ctgaggaagt ggtccttgat gaatatgact atcggtataa ccgaccaaaa agagaacccg 1380 tatcccttac cctagctgta atgctcggat tagggacggc cgttggcgta ggaacaggga 1440 cagctgccct gatcacagga ccacagcagc tagagaaagg acttggtgag ctacatgcgg 1500 ccatgacaga agatctccga gccttagagg agtctgttag caacctagaa gagtccgtga 1560 cttctttatc tgaagtggtt ctacagaacc gga 1593 <210> 74 <211> 1593 <212> DNA <213> porcine endogenous retrovirus <400> 74 atgcatccca cgttaagccg gtgccacctc ccgattcggg gtggagagcc gaaaagactg 60 aaaaccccct taagcttcgc ctccatcgct tggttcctta ctctgtaaat aactcctcaa 120 gttaatggta aacgcccgaa ctcccacaaa cccttatctc tcacctggtt acttactgac 180 tccagtacag gtattactat taacagcact caaggggagg ctcccttagg gacctggtgg 240 cctgagttat atgtctgcct tcgatcggta atccctggtc tcaacgacca ggccacaccc 300 cccgatgtac tccgtgctta tgggttttat gtttgcccag gacccccaaa taatgaagaa 360 tattgtggaa atcctcagga tttcttttgc aagcaatgga gctgcgtaac ttctaatgat 420 gggaattgga aatggccagt ctctcagcaa gacagagtaa gttactcttt tgttaacaat 480 cctaccagtt ataatcaatt taattaccgc catgggaaat ggaaagattg gcaacagcag 540 gtacaaaaag atgtacgaaa taagcaaata agctgtaatt tgttagacct agattactta 600 aaaataagtt tcactgaaaa aggaaaacaa gaaaatattc aaaagtgggt aaatggtatg 660 tcttggggaa tagtgtacta tggaggctct gggagaagga aaggatctgt tctaactatt 720 cgcctcagaa tagaaactcg gatggaacct ccggttgcta taggaccaaa taagtgtttg 780 gccgaacaag gacctccaat ccaagaacag aggccatctc ttaacccctc tgattacaat 840 acagcctctg gatcagtccc cactgagcct aacatcacta ttaaaacagg ggcaaaactt 900 tttagcctca tccagggagc ttttcaagct cttaactcca caactccaga ggctacctct 960 tcttgttggc tttgcttagc ttcaggccca ccttactatg aggaacggct agaggaggga 1020 aattcaatgt gacaaaggaa catagagacc aatgtacatg gggatcccaa aataagctta 1080 cccttactga ggtttctgga aaaggcacct gcatagggag ggttccccca tcccaccaac 1140 acctttgtaa ccacactgaa gcctttaatc gaacctctga gagtcagtat ctggtacccg 1200 gttatgacag gtggtgggca tgtaatactg gattaacccc ttgtgtttcc accttggttt 1260 tcaaccaaac taaagacttt tgcgttatgg tccaaattgt cccccgggtg tactactatc 1320 ccgaaaaagc ggtccttgat gaatatgact atagatataa tcagccaaaa agagagccca 1380 tatccctgac actagctgta atgctcggat tgggagtggc tgcaggcgtg ggaacaggaa 1440 cggctgccct aatcacagga ccacaacagc tggagaaagg acttagtgac ctacatcgaa 1500 ttgtaacgga agatctccaa gccctagaaa aatctgtcag taacctagag gaatccctaa 1560 cctccttatc tgaagtggtt ctacagaaca gga 1593 <210> 75 <211> 1593 <212> DNA <213> porcine endogenous retrovirus <400> 75 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaagactga 60 gaatcccctt aagcttcgcc tccatcgtct gcgttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagttcg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttccgg ggaatccttc tgtaggagat ggagctgcgt cacctccagc 420 gatggagact ggaaatggcc gatctctctc caggaccagg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag atatgagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg ggggacagaa ccccctttgg cagtgggacc 720 cgataaagta ctggctgaac aggggccccc ggccctggag ccaccgcata acttgccggt 780 gccccaatta acctcgctgc ggctgacata acacagccgc ctagcaacgg taccactgga 840 tgatcctacc acacgcctag aaactcccca ggtgttcctg ttaagacagg acagagactc 900 tcagtctcat ccagggagct ttccaagcca tcaactccac cgaccccgat gccacttctt 960 cttgttggct ttgtctatcc tcagggcctc cttattatga ggggatggct aaagaaggaa 1020 aattcaatgt gaccaaagag catagaaatc aatgtacatg ggggtcccga aataagctta 1080 ccctcactga agtttccggg aaggggacat gcataggaaa agctccccca tcccaccaac 1140 acctttgcta tagtactgtg gtttatgagc aggcctcaga aaatcagtat ttagtacctg 1200 gttataacag gtggtgggca tgcaatactg ggttaacccc ctgtgtttcc acctcagtct 1260 tcaaccaatc caaagatttc tgtgtcatgg tccaaatcgt cccccgagtg tactaccatc 1320 ctgaggaagt ggtccttgat gaatatgact atcggtataa ccaaccaaaa agagaaccca 1380 tatcccttac cctagctgta atgctcggat tagggacggc cgttggcgta ggaacaggga 1440 cagctgccct gatcacagga ccacagcagc tagagaaagg acttggtgag ctgcatgcgg 1500 ccatgacaga agatctccga gccttagagg agtctgttag caacctagaa gagtccctga 1560 cttctttgtc tgaagtggtt ctacagaaca gga 1593 <210> 76 <211> 1594 <212> DNA <213> porcine endogenous retrovirus <400> 76 atgcatccca cgttaagccg gcgccacctc ccgactcggg gtggagagct gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccaa 120 gccagtggta aacgccttat agacagctcg aacccccgta gacccttatc ccttacctgg 180 ctgattattg accctcatac gggtgtcact gttaatagca ctcgaggtgt cgctcctaaa 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgctaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttccgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg ggaagtacaa agttatggaa ctatataaag ataagagctg ttccccatca 540 gacttagatt atctaaaaat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggaggggg agcagggtcc 660 actttaacca ttcgccttag ggtagagacg gggacggaac cccctgtggc agtgggaccc 720 aacaaagtac tggctgaaca ggggcccccg gccctggagc caccacacaa cttgccggtg 780 ccctaattaa cctcgctgcg gcctgacata acacagccgc ctggcaacgg taccactgga 840 ttgattccta ccaacatgcc tagaaactcc cccggtgttc ctgttaagac aggacagaga 900 ctctttagtc tcatccaggg agttttccaa gccatcaact ccaccaactc agatgccact 960 tcttcttgtt ggctttgtct atcctcaggg cttccttatt atgagagaat ggctagagaa 1020 gggaaattca atgtgaccaa agagcataga aatcaatgta catgggggtc ccgaaataag 1080 cttaccttca ctgaagtttc cgggaagggg acatgcatag gaaaagctcc cccatcccac 1140 caacaccttt gcaatagtac tatggtttat gagcaggcct cagaaaatca gtatttagta 1200 cctggttata acaggtggtg ggcatgcaat actgggttaa ctccctgtgt ttctaccaca 1260 gtctttaacc aatccaaaga tttctgtgtc atggtccagc tcgtcccccg ggtatactac 1320 catcctgagg aagtagtcat cgatgagtat gactattggc ctacccgctc aaaaagagag 1380 cccgtgaccc ttaccctagc cgttatactt agattaggga tggccggtgt ggggacaggg 1440 acagctgccc tgatcacagg accacagcag ctagaaaaag gacttggtga gctacatgca 1500 gccatgacag aagatctccg agccttagag gaatctgtta gcaacctaga agaatccctg 1560 acttctttgt ctgaagtggt tctacagaac agga 1594 <210> 77 <211> 1597 <212> DNA <213> porcine endogenous retrovirus <400> 77 atgcatccca cgttaacctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtaaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg caccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggaggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacggaac cccctgtggc agtgggaccc 720 aacatagtac tggctgaaca ggggcccccg gccctggagc caccacacaa cttgccggtg 780 ccccagttaa cctcgctgcg gcctgacata acacagccgc ctgacaacag taccactgga 840 ttgatttcct accaacacgc ctagaaactc ccccggtgtt cctgttaaga caggacagag 900 actcttcagt ctcatccagg gagctttcca agccatcaac tccaccgacc ccgatgccac 960 ttctccttgt tggctttgtc tatcctcagg gcctccttat tatgagggaa tggctagaga 1020 agggaaattc aatgtgacca aagagcatag aaatcaatgt acatgggggt cccgaaataa 1080 gctcaccctc actgaagttt ccgggaaggg gacatgcata ggaaaagctc ccccatccca 1140 ccaacacctt tgcaatagta ctatggttta tgagcaggcc ttagaaaatc agtatttagt 1200 acctggttat aactgatggt gggcatgcaa tactgggtta actccctgtg tttctaccac 1260 agtctttaac caatccaaag atttctgtgt catggtccag ctcgtcccct gggtacacta 1320 ccatcctgag gaagtagtca tcgatgagta tgactatcgg cctacccgct caaaaagaga 1380 gcccgtgacc cttaacccta gccgttatac taggattagg gatggctggt gtggggacag 1440 ggacagctgc cctgatcaca gaaccacagc agctagaaaa aggacttggt gagctacagg 1500 cggccataac agaagatctc cgagccttag aggaatctgt taacaaccta gaagaatccc 1560 tgacttctct tgtctgaagt ggttctacag aacagga 1597 <210> 78 <211> 1597 <212> DNA <213> porcine endogenous retrovirus <400> 78 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacagaac cccctgtggc agtgggaccc 720 gataaagtac tggctgaaca ggggcccccg gccctggggc caccgcataa cttgccggtg 780 ccccaattaa cctcgctgcg gcctgacata acacagccgc ctagcaacgg taccactgga 840 ttgattccta ccaacacgcc tagaaactcc ccaggtgttc ctgttaagac aggacagaga 900 ctcttcagtc tcatccaggg agctttccaa gccatcaact ccaccgaccc tgatgccact 960 tcttcttgtt ggctttgtct atcctcaggg cctccttatt atgaggggat ggctaaagaa 1020 ggaaaattca atgtgaccaa agagcataga aatcaatgta catgggggtc ccgaaataag 1080 cttaccctca ctgaagtttc cgggaagggg acatgcatag gaaaagctcc cccatcccac 1140 caacaccttt gctatagtac tgtggtttat gagcaggcct cagaaaatca gtatttagta 1200 cctggttata acaggtggtg ggcatgcaat actgggttaa ccccctgtgt ttccacctca 1260 gtcttcaacc aatccaaaga tttctgtgtc atggtccaaa tcgtcccccg agtgtactac 1320 catcctgagg aagtggtcct tgatgaatat gactatcggt ataaccgacc aaaaagagaa 1380 cccgtatccc ttaccctagc tgtaatgctc ggattaggga cggccgttgg cgtaggaaca 1440 gggacagctg ccctgatcac aggaccacag cagctagaga aaaggcttgg tgagctacat 1500 gcggccatga cagaagatct ccgagcctta gaggagtctg ttagcaacct agaagtgtcc 1560 ctgacttctt tatctgaagt ggttctacag aaccgga 1597 <210> 79 <211> 1594 <212> DNA <213> porcine endogenous retrovirus <400> 79 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc caccatcggc aggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagacagac 360 acacactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agcgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacagaac cccctgtggc agtgggaccg 720 ataaaatact ggctgaacag gggcccccgg ccctggagcc accgcataac ttgccggtgc 780 cccaattacc tcgctgcggc tgacataaca cagccgccta gcaacggtac cactggattg 840 atcctaccaa cacgcctaga aactccccag gtgttcctgt aagacaggac agagactctt 900 cagtctcatc cagggagctt ttcaagccat caactccacc gaccccgatg ccacttcttc 960 ttgttggctt tgtctatcct cagggcctcc cttattatga ggggatggct aaagaaggaa 1020 aattcaatgt gaccaaagag catagaaatc aatgtacgtg ggggtcccga aataagctta 1080 ccctcactga agtttccggg aaggggacat gcataggaaa agctccccca tcccaccaac 1140 acctttgcta tagtactgtg gtttatgagc aggcctcaga aaatcagtat ttagtacctg 1200 gttataacag gtggtgggca tgcaatactg ggttaacccc ctgtgtttcc acctcagtct 1260 tcaaccaatc caaagatttc tgtgtcatgg tccaaatcgt cccccgagtg tactaccacc 1320 ctgaggaagt ggtccttgat gaatatgact atcggtataa ccgaccgaaa agagaacccg 1380 tatcccttac cctagctgta atgctcggat tagggacggc cgttggcgta ggaacaggga 1440 cagctgccct gatcacagga ccacagcagc tagagaaagg acttggtgta gactacatgc 1500 ggccatgaca gaagatctcc gagccttaga ggaggctgta gcaacctaga agagtccctg 1560 acttctttgt ctgaagtggt tctacagaac cgga 1594 <210> 80 <211> 1597 <212> DNA <213> porcine endogenous retrovirus <400> 80 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggagagcc gaaaagaccg 60 aaaatcccct taagcttcgc ctccatcgct tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgc ggacagcccg aacttccata aacccttatc tctcacctgg 180 ttacttactg actccagtac aggtattact attaacagca ctcaagggga ggctccctta 240 gggacctgag ttatatgtct gccttcgatc ggtaatccct ggtctcaacg accaggccac 300 atcccctgat gtactccgtg cttacgggtt taatgtttgc ccaggacccc caaataatga 360 agaatattgt ggaaatcctc aggatttctt ttgcaagcaa gggagctgcg taacttctaa 420 tgatgggaat tggaaatggc cagtctctca gcaagacaga gtaagttact cttttgttaa 480 cagtcctacc agttataatc aatttaatta tggccatggg agatggaaag attggcaaca 540 gcgggtacaa aaagatgtat gaaataagca aataagctgt aattcgttag atctagatta 600 cttaaaaata agttttactg aaaaaggaaa acaagaaaat attcaaaagt gggtaaatgg 660 tatgtcttgg ggaataatgt actatggagg cactgggaga aggaaaggat ctgttctgac 720 tattcgcctc agaatagaag ctcaggtgga acctccggtt gctataggac caaataaggg 780 tttggctgaa caaggacctc caatccaaga acagaggcca tctcctaacc cctctgttta 840 caatacaacc tctggattag tcccccctga gcctaacttc actattaaac aggggcaaaa 900 cttttaagcc tcatccaggg agcttttcaa gctcttaact ccacgactcc agaggctacc 960 tcttcttgtt ggctttgctt agctttgagc ccaccttact atgagggaat ggctagagga 1020 gggaaattca atgtgacaaa ggaacataga gaccaatgta catggggatc ccaaaataag 1080 cttaccctta ctgaggtttc tggaaaaggt acctgcatag ggggggttcc cccatcccac 1140 caacaccttt gtaactacac tgaagccttt aatcgaacct ctgagagtca gtacctggta 1200 cctggttatg acaggtggtg ggcatgtaat actggattaa ccccttgtgt ttccaccctg 1260 gttttcaacc aaactaaaga cttttgtgtt atggtccaaa ttgtcccccg ggtgtactac 1320 tatcccgaaa aagcggtcct tgatgaatat gactatagat ataatcggcc aaaaagagaa 1380 cccatatccc tgacactagc tgtaatgctc ggattgggag tggctgcagg cgtgggaaca 1440 ggaacggctg ccctaatcac aggaccacaa cagctggaga aaggacttag tgacctacat 1500 cgaattgtaa cggaagatct ccaagcccta gaaaaatctg tcagtaacct agaggaatcc 1560 ctaacctcct tatctgaagt ggttctacag aacagga 1597 <210> 81 <211> 1597 <212> DNA <213> porcine endogenous retrovirus <400> 81 atgcatccca cgttaagctg gcgccacctc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agcgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggcggggg agcagggtcc 660 actttaacca ttcgccttag gatagagacg gggacagaac cccctgtggc agtgggaccc 720 gataaaatac tggctgaaca ggggcccccg gccctggagc caccgcataa cttgccggtg 780 ccccaattaa cctcgctgcg gcctgacata acacagccgc ctagcaacgg taccactgga 840 ttgattccta ccaacacgcc tagaaactcc ccaggtgttc ctgttaagac aggacagaga 900 ctcttcagtc tcatccaggg agctttccaa gccatcaact ccaccgaccc tgatgccact 960 tcttcttgtt ggctttgtct atcctcaggg cctccttatt atgaggggat ggctaaagaa 1020 ggaaaattca atgtgaccaa agagcataga aatcaatgta cgtgggggtc ccgaaataag 1080 cttaccctca ctgaagtttc cgggaagggg acatgcatag gaaaagctcc cccatcccac 1140 caacaccttt gctatagtac tgtggtttat gagcaggcct cagaaaatca gtatttagta 1200 cctggttata acaggtggtg ggcatgcaat actgggttaa ccccctgtgt ttccacctca 1260 gtcttcaacc aatccaaaga tttctgtgtc atggtccaaa tcgtcccccg agtgtactac 1320 caccctgagg aagtggtcct tgatgaatat gactatcggt ataaccgacc gaaaagagaa 1380 cccgtatccc ttaccctagc tgtaatgctc ggattaggga cggccgttgg cgtaggaaca 1440 gggacagctg ccctgatcac aggaccacag cagctagaga aaggacttgg tgagctacat 1500 gcggccatga cagaagatct ccgagcctta gaggaggctg ttagcaacct agaagagtcc 1560 ctgacttctt tgtctgaagt ggttctacag aacagga 1597 <210> 82 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 82 atgcatccca cgttaagccg gtgaaacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttagtggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctccctta 240 gggacctggt ggcctgagtt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt atgtttgccc aggatcccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgttttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgt tataggacca 780 aataaggggt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaac tttttagcct aatccaggga acttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgagaaaatg 1020 gctagaggag gaaaattcaa tgtgacaaag aaacatagag accaatgtac atagggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaagaca cctgcatagg gagggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag 1200 tatctggtac ccggttatga caggtggtgg acatgtaata ctggattaac cccttgtgtt 1260 tccaccctgg ttttcaacca aactaaagac ttttgcgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa aacggtcctt gataaatatg actatagata taatcggcca 1380 aaaagagaac ccatatccct gacactagct gtaatgctcg gactgggagt ggctgcaggc 1440 gtgggaacag gaacggctgc cctaatcaca ggaccacaac agctagaaaa aggaattagt 1500 gacctacatc gaattgtaac aaaagatctc caagccctaa aacaatctgt cagtaaccta 1560 gaggaatccc taacctcctt atctgaagtg gttctacaga acagga 1606 <210> 83 <211> 1605 <212> DNA <213> porcine endogenous retrovirus <400> 83 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggagagcc gaaaaaactg 60 aaaatcccct taagcttcgc ctccatcgct tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgc ggacagcccg aacttccata aacccttatc tctcacctgg 180 ttacttactg actccagtac aggtattact attaacagca ctcaagggga ggctccctta 240 gggacctggt ggcctgagtt atatgtctgc cttcgatcgg taatccctgg tctcaacgac 300 caggccacac tctctgatgt actccgtgct tacgggttta atgtttgccc aggacccccc 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaagg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca gtcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa aggtgtacga aataagcaaa taagctgtaa ttcgttagat 600 ctagattact taaaaataag ttttactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aataatgtac tatggaggca ctgggagaag gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggctgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgtttaca atacaacctc tggattagtc ccccctgagc ctaacttcac tattaaaaca 900 ggggcgaaac tttttagcct catccaggga gcttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gctttgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtttct ggaaaaggca cctgcatagg gagggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag 1200 tacctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccctgg ttttcaacca aactaaagac ttttgtgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagaac ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc 1440 gtggaaacag gaacggctgc cctaatcaca ggaccacaac agctggagaa aggacttagt 1500 gacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaaccta 1560 gaggaatccc taacctcctt gtctgaagtg gttctacaga acaga 1605 <210> 84 <211> 1594 <212> DNA <213> porcine endogenous retrovirus <400> 84 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggagagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgct tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgc ggacagcccg aacttccata aacccttatc tctcacctgg 180 ttacttactg actccagtac aggtattact attaacagca ctcaagggga ggctccctta 240 gggacctgag ttatatgtct gccttcgatc ggtaatccct ggtctcaacg accaggccac 300 atcccctgat gtactccgtg tttacgggtt taatgtttgc ccaggacccc caaataatga 360 agaatattgt ggaaatcctc aggatttctt ttgcaagcaa gggagctgcg taacttctaa 420 tgatgggaat tggaaatggc cagtctctca gcaagacaga gtaagttact cttttgttaa 480 cagtcctacc agttataatc aatttaatta tggccatggg agatggaaag attggcaaca 540 gcgggtacaa aaagatgtat gaaataagca aataagctgt aattcgttag atctagatta 600 cttaaaaata agttttactg aaaaaggaaa acaagaaaat attcaaaagt gggtaaatgg 660 tatgtcttgg ggaataatgt actatggagg cactgggaga aggaaaggat ctgttctgac 720 tattcgcctc agaatagaag ctcagatgga acctccggtt gctataggac caaataaggg 780 tttggctgaa caaggacctc caatccaaga acagaggcca tctcctaacc cctctgttta 840 caatacaacc tctggattag tccccctgag cctaacttca ctattaaaac aggggcaaac 900 tttttagcct catccaggga gcttttcaag ctcttaactc cacgactcca gaggctacct 960 cttcttgttg gctttgctta gctttgagcc cacgtactat gaggaatggc tagaggaggg 1020 aaattcaatg tgacaaagga acatagagac caatgtacat ggggatccca aaataagctt 1080 acccttactg aggtttccgg aaaaggtacc tgcataggga gggttccccc atcccaccaa 1140 cacctttgta actacactga agcctttaat cgaacctctg agagtcagta cctggtacct 1200 ggttatgaca ggtggtgggc atgtaatact ggattaaccc cttgtgtttc caccctggtt 1260 ttcaaccaaa ctaaagactt ttgtgttatg gtccaaattg tcccccgggt gtactactat 1320 cccgaaaaag cggtccttga tgaatatgac tatagatata atcggccaaa aagagaaccc 1380 atatccctga cactagctgt aatgctcgga ttgggagtgg ctgcaggcgt gggaacagga 1440 acggctgccc taatcacagg accacaacag ctggagaaag gacttagtga cctgcatcga 1500 attgtaacgg aagatctcca agccctagaa aaatctgtca gtaacctaga ggaatcccta 1560 acctccttat ctgaagtggt tctacagaac agga 1594 <210> 85 <211> 1599 <212> DNA <213> porcine endogenous retrovirus <400> 85 atgcatccca cgttaagccg gtgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaattccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctccctta 240 gggacctggt ggcctgaggt atatgtctgc cttcgatcag taatccctgg tctcagtgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt atgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat gcaaaagtgg 660 gtaaatggta tgttttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggactccaa tccaagaaca gaggccatct cctaacccct 840 ctgattacaa tacaacctct ggatcagtcc ccactgagcc taacatcact attaaaccag 900 gggcgaaact ttttagcctc atccagggag cttttcaagc tcttaactcc acgactccag 960 aggctacctc ttcttgttgg ctttgcttag cttcgggccc accttactat gagggaatgg 1020 ctagaggagg gaaattcaat gtgacaaagg aacatagaga cccatgtaca tggggatccc 1080 aaaataagct tacccttact gaggtgtctg gaaaaggcac ctgccatagg gagggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag 1200 tatctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccttgg ttttcaacca aactaaagac ttttgcgtta tggtccaaat tgtcccccgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagagc ccatatccct gacactagct gtaatgctcg gattgggagt ggctgcaggc 1440 gtgggaacag gaacggctgc cctaatcaca ggaccacaac agctggagaa aggacttagt 1500 aaactacctc gaattgtaac ggaagatctc caagccctga aaaatctgtc agtagcctga 1560 gatgctactc ataatctgaa gtggttctac agaacagaa 1599 <210> 86 <211> 1596 <212> DNA <213> porcine endogenous retrovirus <400> 86 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggagagcc gaaaaaactg 60 aaaatcccct taagcttcgc ctccatcgct tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgc ggacagcccg aacttccata aacccttatc tctcacctgg 180 ttacttactg actccagtac aggtattact attaacagca ctcaagggga ggctccctta 240 gggacctggt ggcctgagtt atatgtctgc cttcgatcgg taatccctgg tctcaacgac 300 caggccacac cccctgatgt actccgtgct tacgggttta atgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaagg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca gtcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa aggtgtacga aataagcaaa taagctgtaa ttcgttagat 600 ctagattact taaaaataag ttttactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aataatgtac tatggaggca ctgggagaag gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggctgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgtttaca atacaacctc tggattagtc cccctgagcc taacttcact attaaaacag 900 ggcgaaactt ttagcctcat ccaggagctt ttcaagctct taactccacg actccagagg 960 ctacctcttc ttgttggctt tgcttagctt tgggcccacc ttactatgag ggaatggcta 1020 gaggagggaa attcaatgtg acaaaggaac atagagacca atgtacatgg ggatcccaaa 1080 ataagcttac ccttactgag gtttctggaa aaggcacctg catagggagg gttcccccat 1140 cccaccaaca cctttgtaac cacactgaag cctttaatcg aacctctgag agtcaatacc 1200 tggtacctgg ttatgacagg tggtgggcat gtaatactgg attaacccct tgtgtttcca 1260 ccctggtttt caaccaaact aaagactttt gtgttatggt ccaaattgtc ccccgggtgt 1320 actactatcc cgaaaaagca gtccttgatg aatatgacta tagatataat cggccaaaaa 1380 gagaacccat atccctgaca ctagctgtaa tgctcggatt gggagtggct gcaggcgtgg 1440 aaacaggaac ggctgcccta atcacaggac cacaacagct ggagaaagga cttagtgaca 1500 atacatcgaa ttgtaacgga agatctccaa gccctagaaa aatctgtcag taactagaga 1560 tctactctga aactgaagtg gttctacaga acagaa 1596 <210> 87 <211> 1595 <212> DNA <213> porcine endogenous retrovirus <400> 87 atgcatccca cgttaagctg gtgccacgtc ccgactcggg gtggagagcc gaaaagactg 60 agaatcccct taagctttgc ctccatcgcc tggttcctta ctctaacaat aactccccag 120 gccagtagta aacgccttat agacagctcg aacccccata gacctttatc ccttacctgg 180 ctgattattg accctgatac gggtgtcact gtaaatagca ctcgaggtgt tgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccctgc tgttaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttctgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg gcaagtacaa agtgatgaaa ctatataaag ataagagctg ctccccatca 540 gacttagatt atctaaagat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggaggggg agcagggtcc 660 actttaacca tttgccttag gatagagacg gggacggaac ccccagtggc agtgggatcc 720 aacaaagtac tggctgaaca ggggcctccg gccctggagc caccacacaa cttgctggtg 780 ccccaattaa cctcgctgcg gcctgacata acacagctgc ctgacaacgg taccactggg 840 attgattcct accaacatgc ctagaaactc cccgggtgtt cctgttaaga caggacagag 900 actcttcagg ctcatccagg gagctttcca agccatcacc tccacggacc ccgatgccac 960 ttcttcttgt tggctttgtc tatcctcagg gcctccttat tatgagggaa tggctagaga 1020 aggaaaattc gatgtgacta aagagcatag aaatcaatgt acatgggggt cccgaaataa 1080 gcttaccctc actgaagttt ccgggaaggg gacatgcata ggaaaagctc cccatcccac 1140 caacaccttt gcaatagtac tatggtttat gagcaggcct cagaaaatca gtatttagta 1200 cctggttata acaggtggtg ggcatgcaat actgggttaa ctcccctgtg tttctaccac 1260 agtctttaac caatccaaag atttctgtgt catggtccag ctcatccccc aggtatacta 1320 ccatcctgag gaagtagtca tcgatgagta tgactatcgg cctacccgct caaaaagaga 1380 gcctgtgacc ctcaccctag ccattatact tggattaggg atggccggtg tggggacagg 1440 gacagctgcc ctgatcacag gaccacagca gctagaaaaa ggacttggtg agctacatgc 1500 ggccatgaca gaagatctcc gagccttaga ggaatctgtt agcaacctag aagaaaccct 1560 gacttcttgg tctgaagtgg ttctacagaa cagaa 1595 <210> 88 <211> 1594 <212> DNA <213> porcine endogenous retrovirus <400> 88 atgcatccca cgttaagctg gcgcctcgtc ccgactcggg gtggagagct gaaaagactg 60 agaatcccct taagcttcac ctccatcgcc tggttcctta ctctaacaat aactccccaa 120 gccagtggta aacgccttat agacagctcg aacccccgta gacccttatc ccttacctgg 180 ctgattattg accctcatac gggtgtcact gttaatagca ctcgaggtgt cgctcctaga 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgctaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttccgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcctgg ggaagtacaa agttatggaa ctatataaag ataagagctg ttccccatca 540 gacttagatt atctaaaaat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggaggggg agcagggtcc 660 actttaacca ttcgccttag ggtagagacg gggacggaac cccctgtggc agtgggaccc 720 gacaaagtac tggctgaaca ggggcccccg gccctggagc caccacacaa cttgccggtg 780 ccctaattaa cctcgctgcg gcctgacata acacagccgc ctggcaacgg taccactgga 840 ttgattccta ccaacatgcc tagaaactcc cccggtgttc ctgttaagac aggacagaga 900 ctctttagtc tcatccaggg agttttccaa gccatcaact ccaccaactc cgatgccact 960 tcttcttgtt ggctttgtct atcctcaggg cttccttatt atgagagaat ggctagagaa 1020 gggaaattca atgtgaccaa agagcataga aatcaatgta catgggggtc ccgaaataag 1080 cttaccttca ctgaagtttc cgggaagggg acatgcatag gaaaagctcc cccaccccac 1140 caacaccttt gcaatagtac tatggtttat gagcaggcct cagaaaatca gtatttagta 1200 cctggttata acaggtggtg ggcatgcaat actgggttaa ctccctgtgt ttctaccaca 1260 gtctttaacc aatccaaaga tttctgtgtc atggtccagc tcgtcccccg ggtatactac 1320 catcctgagg aagtagtcat cgatgagtat gactatcggc ctacccgctc aaaaagagag 1380 cccgtgaccc ttaccctagc cgttatactt ggattaggga tggccggcgt ggggacaggg 1440 acagctgccc tgatcacagg accacagcag ctagaaaaag gacttggtga gctacatgca 1500 gccatgacag aagatctccg agccttagag gaatctgtta gcaacctaga agaatccctg 1560 acttctttat ctgaagtggt tctacagaac cgga 1594 <210> 89 <211> 1603 <212> DNA <213> porcine endogenous retrovirus <400> 89 atgcatccca cgttaaggcg gcgccacctt ccgattcggg gtggaagccg aaagactgaa 60 aatcccctta agcttcgcct ccatcgcgtg gtccatactc tgtcaataac tcctcaagtt 120 aatggtaaaa gcattgtgga cagcccgaac tcccataaac ccttatctct cacctggtta 180 cttactgact ccggtacagg tattaatatt aacagcactc aaggggagac tcccttgggg 240 acctggtggc ctgaattata tgtctgcctt cgatcagtaa tccctggtct caatgaccag 300 gccacacccc ccgatgtact ccgtgcttac gggttttacg tttgcccagg acccccaaat 360 aatgaagaat attgtggaaa tcctcaggat ttcttttgca agcaatggag ctgcgtaact 420 tctaatgatg ggaattggaa atggccagtc tctcagcaag acagagtaag ttactctttt 480 gttaacaatc ctaccagtta taatcaattt aattatggcc atgggagatg gaaagattgg 540 caacagcggg tacaaaaaga tgtacgaaat aagcaaataa gctgtcattc gttagaccta 600 gattacttaa aaataagttt cactgaaaaa ggaaaacaag aaaatattca aaagtgggta 660 aatggtatgt cttggggaat agtgtactat ggaggctctg ggagaaagaa aggatctgtt 720 ctgactattc gcctcagaat agaaactcag atggaacctc cggttgctat aggaccaaat 780 aagggtttgg ccgaacaagg acctccaatc caagaacaga ggccatctcc taacccctct 840 gattacaata caacctctgg atcagtcccc actgagccta acatcactat taaaacaggg 900 gcgaaacttt ttagcctcat ccagggagct tttcaagctc ttaactccac gactccagag 960 gctacctctt cttgttggct ttgcttagct tcgggcccac cttactatga gggaatggct 1020 agaggaggga aattcaatgt gacaaaggaa catagagacc aatgtacatg gggatcccaa 1080 aataagctta cccttactga ggtttctgga aaaggcacct gcatagggat ggttccccca 1140 tcccaccaac acctttgtaa ccacactgaa gcctttaatc gaacctctga gagtcagtat 1200 ctggtacctg gttatgacag gtggtgggca tgtaatactg gattaacccc ttgtgtttcc 1260 accttggttt tcaaccaaac taaagacttt tgcgttatgg tccaaattgt cccccgggtg 1320 tactactatc ccgaaaaagc agtccttgat gaatatgact atagatataa tcggccaaaa 1380 agagagccca tatccctgac actagctgta atgctcggat tgggagtggc tgcaggcgtg 1440 ggaacaggaa cggctgccct aatcacagga ccgcaacagc tggagaaagg acttagtaac 1500 ctacatcgaa ttgtaacgga agatctccaa gccctagaaa aatctgtcag taacctggag 1560 gaatccctaa cctccttatc tgaagtggtt ctacagaacc gaa 1603 <210> 90 <211> 1594 <212> DNA <213> porcine endogenous retrovirus <400> 90 atgcatccca cgttaagctg gcgccacctc ccgactcagg gtggagagct gaaaagactg 60 agaatcccct taagcttcgc ctccatcgcc tggttcctta ctctaacaat aactccccaa 120 gccagtggta aacgccttat agacagctcg aacccccgta gacccttatc ccttacctgg 180 ctgattattg accctcatac gggtgtcact gttaatagca ctcgaggtgt cgctcctaaa 240 ggcacctggt ggcctgaact gcatttctgc ctccgattga ttaaccccgc tgctaaaagc 300 acacctccca acctagtccg tagttatggg ttctattgct gcccaggcac agagaaagag 360 aaatactgtg ggggttccgg ggaatccttc tgtaggagat ggagctgcgt cacctccaac 420 gatggagact ggaaatggcc gatctctctc caggaccggg taaaattctc ctttgtcaat 480 tccggcccgg ggaagtacaa agttatggaa ctatataaag ataagagctg ttccccatca 540 gacttagatt atctaaaaat aagtttcact gaaaaaggaa aacaggaaaa tattcaaaag 600 tggataaatg gtatgagctg gggaatagtt ttttataaat atggaggggg agcagggtcc 660 actttaacca ttcgccttag ggtagagacg gggacggaac cccctgtggc agtgggaccc 720 aacaaagtac tggctgaaca ggggcccccg gccctggagc caccacacaa cttgccggtg 780 ccctaattaa cctcgctgcg gcctgacata acacagccgc ctggcaacgg taccactgga 840 ttgattccta ccaacatgcc tagaaactcc cccggtgttc ctgttaagac aggacagaga 900 ctctttagtc tcatccaggg agttttccaa gccatcaact ccaccaactc cgatgccact 960 tcttcttgtt ggctttgtct atcctcaggg cttccttatt atgagagaat ggctagagaa 1020 ggaaaattca atgtgaccaa agagcataga aatcaatgta catgggggtc ccgaaataag 1080 cttaccttca ctgaagtttc cgggaagggg acatgcatag gaaaagctcc cccatcccac 1140 caacaccttt gcaatagtac tatggtttat gagcaggcct cagaaaatca gtatttagta 1200 cctggttata acaggtggtg ggcatgcaat actgggttaa ctccctgtgt ttctaccaca 1260 gtctttaacc aatccaaaga tttctgtgtc atggtccagc tcgtcccccg ggtatactac 1320 catcctgagg aagtagtcat cgatgagtat gactattggc ctacccgctc aaaaagagag 1380 cccgtgaccc ttaccctagc cgttatactt agattaggga tggccggtgt ggggacaggg 1440 acagctgccc tgatcacagg accacagcag ctagaaaaag gacttggtga gctacatgca 1500 gccatgacag aagatctccg agccttagag gaatctgtta gcaacctaga agaatccctg 1560 acttctttgt ctgaagtggt tctacagaac agga 1594 <210> 91 <211> 1604 <212> DNA <213> porcine endogenous retrovirus <400> 91 atgcatccca cgttaaggcg gcgccacctc ccgattcggg gtagagatct gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgct tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccagtac aggtattact attaacagca ctcaagggga ggctccctta 240 aggacctggt ggcctgagtt atatgtctgc cttggatcgg taatccctgg tctcaacgac 300 caggccacac cccccgatgt actccatgct tacaggtttt atgtttgccc aggaccccca 360 aataataaaa aatactgtga aaatcctcag gatttctttt acaaacaata gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcaac aagacagagt aagttactct 480 tttgttaaca atcctaccac ttataatcaa tttaattatg gccatgggag ataaaaagat 540 tgacaacagc gggtacaaaa agatgtacaa aataagcaaa taagctgtaa tttgttagac 600 ctagattact taaaaataag tttcactgaa aaaaaaaaca agaaaatatt caaaagtggg 660 taaatggtat gtcttgaaaa ataatgtact atggaggctc tgggaaaagg aaaggatcta 720 ttctaactat tcgcctcaga atacaaactc agatggaacc tccggttgct ataggaccaa 780 ataagggttt ggccgaacaa ggacctccaa tccaagaaca gaggccatct cctaacccct 840 ctgattacaa tacaacctct ggatcagtcc ccactgagcc taacatcact attaaaacag 900 gggcgaaact ttttagccta atccagggaa cttttcaagc tcttaactcc acgactccag 960 aggctacctc ttcttgttgg ctttgcttag cttcgggccc accttactat gagaaaatgg 1020 ctagaggagg gaaattcaat gtgacaaaga aacatagaga ccaatgtaca tagggatccc 1080 aaaataagct tacccttact gaggtttctg gaaaagacac ctgcataggg agggttcccc 1140 catcccacca acacctttgt aaccacactg aagcctttaa tcgacctcta agagtcagta 1200 tctggtaccc ggttatgaca tgtggtggac atgtaatact ggattcaccc cttgtgtttc 1260 caccctggtt ttcaaccaaa ctaaagactt ttgcgttatg gtccaaattg tcccccgggt 1320 gtactactat cccgaaaaaa cggtccttga taaatatgac tatagatata atcggccaaa 1380 aagagaaccc atatccctga cactagctgt aatgctcgga ctgggagtgg ctgcaggcgt 1440 gggaacagga acggctgccc taatcacagg accacaacag ctagaaaaag gaattagtga 1500 cctacatcga attgtaacaa aagatctcca agccctaaaa caatctgtca gtaacctaga 1560 ggaatcccta acctccttat ctgaagtggt tctacagaac agga 1604 <210> 92 <211> 1606 <212> DNA <213> porcine endogenous retrovirus <400> 92 atgcatccca cgttaagccg gcgccacctc ccgattcggg gtggaaagcc gaaaagactg 60 aaaatcccct taagcttcgc ctccatcgcg tggttcctta ctctgtcaat aactcctcaa 120 gttaatggta aacgccttgt ggacagcccg aactcccata aacccttatc tctcacctgg 180 ttacttactg actccggtac aggtattaat attaacagca ctcaagggga ggctcccttg 240 gggacctggt ggcctgagtt atatgtctgc cttcgatcag taatccctgg tctcaatgac 300 caggccacac cccccgatgt actccgtgct tacgggtttt acgtttgccc aggaccccca 360 aataatgaag aatattgtgg aaatcctcag gatttctttt gcaagcaatg gagctgcgta 420 acttctaatg atgggaattg gaaatggcca gtctctcagc aagacagagt aagttactct 480 tttgttaaca atcctaccag ttataatcaa tttaattatg gccatgggag atggaaagat 540 tggcaacagc gggtacaaaa agatgtacga aataagcaaa taagctgtca ttcgttagac 600 ctagattact taaaaataag tttcactgaa aaaggaaaac aagaaaatat tcaaaagtgg 660 gtaaatggta tgtcttgggg aatagtgtac tatggaggct ctgggagaaa gaaaggatct 720 gttctgacta ttcgcctcag aatagaaact cagatggaac ctccggttgc tataggacca 780 aataagggtt tggccgaaca aggacctcca atccaagaac agaggccatc tcctaacccc 840 tctgattaca atacaacctc tggatcagtc cccactgagc ctaacatcac tattaaaaca 900 ggggcgaaac tttttagcct catccaggga gcttttcaag ctcttaactc cacgactcca 960 gaggctacct cttcttgttg gctttgctta gcttcgggcc caccttacta tgagggaatg 1020 gctagaggag ggaaattcaa tgtgacaaag gaacatagag accaatgtac atggggatcc 1080 caaaataagc ttacccttac tgaggtgtct ggaaaaggca cctgcatagg gagggttccc 1140 ccatcccacc aacacctttg taaccacact gaagccttta atcgaacctc tgagagtcag 1200 tatctggtac ctggttatga caggtggtgg gcatgtaata ctggattaac cccttgtgtt 1260 tccaccttgg ttttcaacca aactaaagac ttttgcgtta tggtccaaat tgtcccctgg 1320 gtgtactact atcccgaaaa agcagtcctt gatgaatatg actatagata taatcggcca 1380 aaaagagagc ccatatccct gacactagct gtaatgctcg gattgggaat ggctgcaggc 1440 gtgggaacag gaacggctgc cctaatcaca ggaccgcaac agctggagaa aggacttagt 1500 aacctacatc gaattgtaac ggaagatctc caagccctag aaaaatctgt cagtaacctg 1560 gaggaatccc taacctcctt atctgaagtg gttctacaga acagaa 1606 <210> 93 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> PERV-A1-F <400> 93 taatccctgg tctcaatgac ca 22 <210> 94 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> PERV-A1-R <400> 94 ttctcccaga gcctccatag ta 22 <210> 95 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> PERV-A2-F <400> 95 aagaacagag gccatctcct aa 22 <210> 96 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> PERV-A2-R <400> 96 cgattaaagg cttcagtgtg gt 22 <210> 97 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> PERV-A3-F <400> 97 tctgggagaa agaaaggatc tg 22 <210> 98 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> PERV-A3-R <400> 98 cgattaaagg cttcagtgtg gt 22 <210> 99 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> PERV-B1-F <400> 99 tgattaaccc cgctgttaaa ag 22 <210> 100 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> PERV-B1-R <400> 100 ctaggcggct gtgttatgtc ag 22 <210> 101 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> PERV-B2-F <400> 101 gggcaagtac aaagtgatga aa 22 <210> 102 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> PERV-B2-R <400> 102 caggaacacc tggggagttt ct 22 <210> 103 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> PERV-B3-F <400> 103 gggagcaggg tccactttaa c 21 <210> 104 <211> 24 <212> DNA <213> Artificial Sequence <220> <223> PERV-B3-R <400> 104 tgctcataaa ccacagtact atag 24 <210> 105 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> PERV-F <400> 105 atattcaaaa gtgggtaaat g 21 <210> 106 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> PERV-R <400> 106 gtgttggtgg gatgggggaa c 21

Claims (25)

  1. 서열목록 제1서열 내지 제92서열로 구성된 군으로부터 선택되는 무균 돼지 내인성 레트로바이러스의 env 단백질을 코딩하는 뉴클레오타이드 서열을 포함하는 env 뉴클레오타이드.
  2. 서열목록 제9서열, 제11서열, 제17서열, 제25서열, 제29서열, 제31서열, 제42서열, 제46서열, 제57서열, 제60서열, 제69서열, 제70서열, 제72서열, 제83서열, 제89서열 또는 제92서열에서 418번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  3. 서열목록 제9서열, 제11서열, 제17서열, 제25서열, 제29서열, 제31서열, 제42서열, 제46서열, 제57서열, 제60서열, 제69서열, 제70서열, 제72서열, 제83서열, 제89서열 또는 제92서열에서 672번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  4. 서열목록 제9서열, 제29서열, 제31서열, 제42서열, 제57서열, 제72서열, 제83서열 또는 제92서열에서 1133번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  5. 서열목록 제11서열, 제25서열, 제46서열, 제49서열 또는 제60서열에서 917번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  6. 서열목록 제29서열, 제31서열 또는 제83서열에서 46번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  7. 서열목록 제29서열, 제31서열, 제83서열 또는 제89서열에서 56번째 뉴클레오 타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  8. 서열목록 제29서열, 제31서열 또는 제83서열에서 140번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  9. 서열목록 제29서열, 제31서열 또는 제83서열에서 196번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  10. 서열목록 제29서열, 제31서열 또는 제83서열에서 209번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  11. 서열목록 제29서열, 제31서열 또는 제83서열에서 340번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  12. 서열목록 제29서열, 제31서열 또는 제83서열에서 491번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  13. 서열목록 제29서열, 제31서열 또는 제83서열에서 589번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  14. 서열목록 제29서열, 제31서열 또는 제83서열에서 685번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  15. 서열목록 제29서열, 제31서열 또는 제83서열에서 700번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  16. 서열목록 제11서열, 제17서열 또는 제60서열에서 745번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  17. 서열목록 제29서열, 제31서열 또는 제83서열에서 845번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  18. 서열목록 제29서열, 제31서열 또는 제83서열에서 866번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  19. 서열목록 제29서열, 제31서열 또는 제83서열에서 874번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  20. 서열목록 제29서열, 제31서열 또는 제83서열에서 886번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  21. 서열목록 제29서열, 제31서열 또는 제83서열에서 995번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  22. 서열목록 제12서열, 제30서열 또는 제43서열에서 28번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  23. 서열목록 제23서열, 제27서열 또는 제50서열에서 829번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  24. 서열목록 제12서열, 제30서열 또는 제43서열에서 902번째 뉴클레오타이드를 포함하는 10-100개의 연속 뉴클레오타이드 잔기 또는 그의 상보적인 뉴클레오타이드 서열을 포함하는 돼지 내인성 레트로바이러스를 동정하는 데 이용되는 유전적 마커.
  25. (a) 돼지로부터 핵산 분자를 분리하는 단계; 및 (b) 상기 제 2 항 내지 제 24 항 중 어느 한 항의 유전적 마커의 존재 유무를 확인하는 단계를 포함하는 돼지 내인성 레트로바이러스의 동정방법.
KR1020060032581A 2005-04-11 2006-04-11 국내 돼지에 존재하는 내인성 레트로바이러스의 엔밸로프유전자 KR100814341B1 (ko)

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