KR20060088090A - Health functional egg affording the high density lipoprotein in human body - Google Patents

Abstract

The present invention replaces inorganic salts such as salts used in chicken feed with silver nano inorganic salts to facilitate the absorption, metabolism and distribution of the chicken body and inhibit the growth of various bacteria in the chicken body by silver nano antibacterial activity to promote metabolism By easily releasing harmful substances into the body, it enhances the health functional properties of eggs produced by chickens, thereby providing functional eggs that increase the content of HDL cholesterol beneficial to the human body when cholesterol contained in egg yolk is ingested in the human body.

Egg, egg yolk, high density, cholesterol, feed, silver nano, inorganic salt, antibacterial activity

Description

Health functional egg affording the high density lipoprotein in human body}

The present invention relates to a healthy functional egg that increases the high density cholesterol content in the human body. More specifically, the present invention relates to a healthy functional egg produced by a chicken fed an inorganic salt substitute with a silver nano inorganic salt and a feed which greatly reduced the amount of antibiotics. The cholesterol contained in the functional functional egg yolk is consumed by the human body. Relates to a healthy functional egg that increases the HDL cholesterol content beneficial.

Cholesterol is a kind of steroid compound widely found as a cellular component of higher animals. It is a component of all animal cells necessary for growth and maintenance, regulates the physical state of biological membranes, and is a precursor of steroid hormones, vitamin D and bile acids. In addition, myelin and plasma lipoproteins of the brain are essential lipids required by the human body.

Such cholesterol is transported through the blood in the form of high density lipoprotein (HDL) called low density cholesterol (LDL) or low density lipoprotein (LDL) called low density cholesterol in the human body is used for metabolism. Low-density lipoproteins are an important blood cholesterol transporter, and because they move cholesterol from the liver to the blood, it increases the content of harmful cholesterol in the blood, while high-density lipoproteins move cholesterol from the blood to the liver to break down cholesterol, and then into the body through bile. It plays a role in promoting excretion. Therefore, more HDL cholesterol is beneficial to the human body. On the contrary, it is known that LDL cholesterol is harmful to the human body, causing arteriosclerosis and causing cerebrovascular and cardiovascular diseases.

In order to prevent various adult diseases such as arteriosclerosis, obesity, hypertension, angina pectoris, myocardial infarction and stroke, various researches are being conducted to reduce cholesterol content, and livestock farmers put various additives such as herbal medicines, antibiotics and bio-ceramic compositions Research has been actively conducted to improve the quality of meat and produce low-cholesterol meat products.

Korean Laid-Open Patent Publication No. 2004-27191 discloses a method for producing cholesterol-reduced eggs by feeding betacyclodextrin to poultry in the form of feed or drinking water. In addition, the Republic of Korea Patent Publication No. 2006-11578 'Poultry feed additives for producing low cholesterol and low cholesterol production method using the same in the poultry cholesterol inhibitors, feed and feed additives comprising the same, low cholesterol using the same Disclosed is a method of producing eggs. On the other hand, the Republic of Korea Patent Publication No. 1998-16882 'Chicken feed composition for egg production with reduced cholesterol content and eggs produced by administering the feed' in the ginkgo leaves, lumps and horsetail to produce eggs with low cholesterol content Disclosed are a chicken feed composition comprising one or more selected furanoid-containing plants, and a method for producing a low cholesterol content egg by administering the feed composition and eggs produced by the method.

However, cholesterol is an essential ingredient for the human body, and simply manufacturing cholesterol-lowering foods cannot impart health functionalities, and lowers the content of LDL cholesterol harmful to the human body and promotes the content of HDL cholesterol, which is beneficial to the human body. New challenges are required.

Technical problem to be achieved in the present invention is to replace the inorganic salts, such as salt used in chicken feed with silver nano inorganic salts to facilitate the absorption, metabolism, distribution, etc. in the chicken body and inhibit the growth of various bacteria in the chicken body by silver nano antibacterial activity By promoting the metabolism of the body by easily releasing harmful substances in the body to improve the health functional properties of the eggs produced by chickens to develop a functional egg that increases the HDL cholesterol content beneficial to the human body when the cholesterol contained in egg yolk will be.

An object of the present invention is to produce a chicken fed a feed composition for poultry containing 0.01 to 3 parts by weight of silver nano-coated inorganic salts, 0.001 to 0.1 parts by weight of antibiotics based on 100 parts by weight of poultry grains containing sugar, protein, etc. In the health functional egg, the health functional egg is to provide a health functional egg which raises the content of cholesterol in the blood to a high density cholesterol (HDL) content of more than 30% by weight of the total cholesterol content when ingested in the human body.

In addition, the silver nano-coated inorganic salts are characterized by containing 0.01 to 0.5 parts by weight of the silver nano compound with respect to 1 part by weight of one or more inorganic salts selected from sodium chloride, calcium chloride, potassium chloride, magnesium chloride, sodium sulfate.

In this case, the silver nano-coated inorganic salts are prepared by vapor-depositing silver nano particles having a particle size of 1 to 50 nm in inorganic salts.

Hereinafter, the present invention will be described in more detail.

The silver nano inorganic salts used in the present invention were prepared by using a method of preparing NAP (Nano Attached Powder), which is a method of depositing 99.99% of the nano-sized silver particles in an inorganic salt under vacuum using physical vapor deposition.

The silver nano inorganic salts prepared by the NAP manufacturing method are capable of producing high purity (99.99%) silver nano particles and easily control the size of the nano particles, thereby depositing 1-50 nm silver nano particles on the refined inorganic salts to provide excellent stability of the nano particles. Is characteristic. In addition, it is sterilized and deodorized by pure silver particles of 50 ppm of silver deposited on inorganic salts.

When these silver nano inorganic salts are contained in the feed, the chickens reduce all harmful bacteria in poultry such as chickens, and also function to selectively sterilize harmful bacteria in the intestine, thereby enhancing metabolism. In addition, silver has no side effects and is released into the body within 3 days after ingestion, thereby activating healthy cells of the living body to increase the immunity of poultry. Therefore, in the case of the present invention, by feeding the silver nano inorganic salts in the feed to the chicken to increase the bactericidal power against the intestinal harmful bacteria and increase the immunity, as well as inducing the proper harmony of cholesterol and lecithin in egg yolk produced by the chicken health functional It will produce an improved egg.

On the other hand, in the case of antibiotics put into the feed is completely digested and discharged after 2 to 3 weeks, the eggs produced before these antibiotics are completely digested, the problem of causing antibiotics in the eggs due to the residual antibiotics present in the chicken body Is being raised. In addition, the increase in antibiotic-resistant bacteria in eggs caused by antibiotic abuse can cause the genetic material containing resistance to be transferred to other bacteria by the transfer of a plasmid conjugation, which increases the resistance of bacteria from eggs to the human body. There is a risk of metastasis. For these reasons, antibiotic restrictions in eggs have recently been severely limited.

Lecithin corresponds to 70 to 80% of egg yolk phospholipids and contains water-soluble phosphate groups and fat-soluble fatty acids, which are commonly used as natural surfactants. Lecithin has the function of breaking down lipids and absorbing and degrading cholesterol precipitated from bile. Therefore, ingesting lecithin can prevent the body's accumulation of cholesterol by breaking down and absorbing cholesterol itself. In addition, lecithin is effective in preventing obesity because it plays a role in suppressing the accumulation of unnecessary fat, preventive effects of miscarriage and stillbirth, diabetes prevention to promote insulin secretion, and anti-cancer effects.

In the case of the healthy functional egg produced in the present invention, the yolk cholesterol is transported and stored in the form of high density cholesterol which is beneficial to the human body due to the action of lecithin in the yolk, thereby playing a beneficial role for the human body. In other words, the lecithin is absorbed and metabolized cholesterol so that it is transported and stored in the form of high density cholesterol that can prevent the invasion of cholesterol in the human body.

The present invention will be described in more detail with reference to the following examples. However, these examples do not limit the scope of the present invention.

Preparation Example 1 Preparation of Silver Nano Inorganic Salt-Containing Feed Composition

A poultry feed composition comprising 0.5 parts by weight of silver nano-coated inorganic salts and 0.01 parts by weight of antibiotics is prepared for 100 parts by weight of poultry grains containing sugar, protein, and the like. At this time, grains containing sugar and protein are prepared by combining grains selected from corn, wheat, soybeans, barley, rice bran, etc. to contain appropriate sugars, proteins and lipids. In addition, the silver nano-coated inorganic salts contain 0.1 parts by weight of silver nano compounds based on 1 part by weight of one or more inorganic salts selected from sodium chloride, calcium chloride, potassium chloride, magnesium chloride, sodium sulfate, etc., wherein the silver nano-coated inorganic salts have a particle size of 5 It is produced by vapor-depositing silver nanoparticles of -30 nm in inorganic salts.

Preparation Example 2 Preparation of Silver Nano Inorganic Salt-Containing Feed Composition

A poultry feed composition comprising 0.6 parts by weight of silver nano-coated inorganic salts and 0.015 parts by weight of antibiotics is prepared based on 100 parts by weight of poultry grains containing sugar, protein, and the like. At this time, grains containing sugar and protein are prepared by combining grains selected from corn, wheat, soybeans, barley, rice bran, etc. to contain appropriate sugars, proteins and lipids. In addition, the silver nano-coated inorganic salts contain 0.15 parts by weight of silver nano compounds with respect to 1 part by weight of one or more inorganic salts selected from sodium chloride, calcium chloride, potassium chloride, magnesium chloride, sodium sulfate, and the like. It is produced by vapor-depositing silver nanoparticles of -30 nm in inorganic salts.

Preparation Example 1 Preparation of Inorganic Salt-Containing Feed Composition

A poultry feed composition comprising 0.5 parts by weight of inorganic salts and 0.01 parts by weight of antibiotics is prepared for 100 parts by weight of poultry grains containing sugar, protein and the like. At this time, grains containing sugar and protein are prepared by combining grains selected from corn, wheat, soybeans, barley, rice bran, etc. to contain appropriate sugars, proteins and lipids. In addition, the inorganic salts are selected from sodium chloride, calcium chloride, potassium chloride, magnesium chloride, sodium sulfate and the like.

Preparation Example 2 Preparation of Feed Composition with Increased Antibiotic Content

A poultry feed composition comprising 0.5 parts by weight of inorganic salts and 0.5 parts by weight of antibiotics is prepared based on 100 parts by weight of poultry grains containing sugar, protein and the like. At this time, grains containing sugar and protein are prepared by combining grains selected from corn, wheat, soybeans, barley, rice bran, etc. to contain appropriate sugars, proteins and lipids. In addition, the inorganic salts are selected from sodium chloride, calcium chloride, potassium chloride, magnesium chloride, sodium sulfate and the like.

Example 1 Generation of Eggs through Feed Intake

As the experimental animals, 50 50-week-old laying hens (ISA brown leghorn) were completely randomly placed in 10 control groups and 10 test groups. Indoor temperature of the experimental animal breeding room was adjusted to 25 ℃, relative humidity 50%, contrast 16L: 8D conditions. Laying hens were fed for 6 weeks by free feeding of water and experimental diets prepared in Examples 1 and 2 and Comparative Examples 1 and 2 until the laying rate was constant. A test group ingesting the feed prepared in Preparation Example 1 is called a first group, and a test group ingesting the feed prepared in Preparation Example 2 is called a second group, and consumed the feed prepared in Preparation Example 1 This test group is called a third group, and the test group ingested the feed prepared in Preparation Example 2 is called a fourth group.

The eggs produced from each test group were obtained and the following tests were conducted.

Example 2 Cholesterol Analysis in Egg Yolk

Egg yolk cholesterol analysis of eggs produced in the four test groups was performed as follows.

2 g of egg yolk isolated from egg white was added at 0.6 ° C by adding 0.6 ml of 33% KOH solution, 9.4 ml of ethyl alcohol (EtOH) and 1 ml of internal standard 5α-cholestan (Sigma C-8300). React for 1 hour. Thereafter, 10 ml of hexane and 5 ml of distilled water were added to the mixture, and the mixture was left to stand. The supernatant was dehydrated with sodium sulfate, and the filtered test solution was analyzed by gas chromatography (Hewlett Packard 6890 Plus, USA). The inlet and detector temperatures of the gas chromatography using an HP-INNOWax column (0.25 μm × 30 m × 0.32 mm) were 330 ° C. and the carrier gas was nitrogen. Results of analysis of cholesterol content are shown in Table 1.

First group 2nd group Third group 4th group Cholesterol content (%) 1.32 ± 0.07 1.28 ± 0.08 1.36 ± 0.09 1.35 ± 0.07

Therefore, the cholesterol content of the first group and the second group ingesting the feed prepared in the preparation example of the present invention is lower than the cholesterol content of the third and fourth groups ingesting the feed prepared in the production comparison example of the present invention. Although the numbers were shown, the difference in cholesterol content could not be recognized. That is, the healthy functional eggs of the present invention did not show a significant decrease in cholesterol content as compared to the eggs produced by chickens fed a conventional feed.

However, the following example was measured whether the change in the high-density cholesterol content in the human body when ingesting the healthy functional eggs of the present invention and the eggs produced by ingesting a conventional feed.

Example 3 Blood Cholesterol Analysis

Forty healthy young people in their twenties were selected and divided into four groups. Ten test panels were set up in one group, and the blood cholesterol content of the panel was measured. After that, the eggs produced in Preparation Example 1 were taken into the test group 1 group, the eggs produced in Preparation Example 2 were taken into the test group 2 group, and produced in Preparation Example 1 in the test group 3 group. Ingested eggs are taken, and the fourth group is fed the eggs produced in Comparative Example 2. Ten panels of the four test groups were ingested five eggs daily for five days, and then the change in cholesterol content in the blood was measured on the sixth day. The cholesterol content in blood was measured by dividing total cholesterol content, low density cholesterol content and high density cholesterol content.

Generally, when the total cholesterol content is 200 mg / dl or less, it is recognized as a healthy normal value, and when the low density cholesterol content is 100 mg / dl or less, it is regarded as a healthy normal value, and when the high density cholesterol content is 50 mg / dl or more, it is regarded as a healthy normal value.

Table 2 shows data for measuring the initial blood cholesterol content and the cholesterol content of the six eggs produced in the first to fourth groups of the test group and the sixth day after the ingestion of eggs.

As shown in Table 2, in the test group ingesting the healthy functional egg of the present invention, the HDL cholesterol content was increased by about 20 to 25% compared to before the egg intake, Only LDL cholesterol content was increased and HDL cholesterol content was not significantly changed.

Therefore, in the case of the test group ingested the normal egg, only the total cholesterol content and LDL cholesterol content increased, it is considered to have a harmful effect on the human body. However, the test group ingesting the egg of the present invention slightly increased the total cholesterol content, but there was no significant change in the LDL cholesterol content and the HDL cholesterol content increased by about 20-25%, which proved to provide beneficial health functionalities to the human body. It is.

The effect of the present invention is to replace the inorganic salts, such as salts used in chicken feed with silver nano inorganic salts to promote metabolism to easily discharge harmful substances in the body to enhance the health functional properties of eggs produced by chickens in egg yolk It is to provide a healthy functional egg that increases the content of HDL cholesterol beneficial to the human body when the cholesterol is ingested in the human body.

Claims (3)

In a healthy functional egg produced by a chicken fed a poultry feed composition comprising 0.01 to 3 parts by weight of silver nano-coated inorganic salts and 0.001 to 0.1 parts by weight of antibiotics based on 100 parts by weight of poultry grains containing sugar and protein. , The healthy functional egg is a healthy functional egg, characterized in that the content of high cholesterol (HDL) in the blood rises to more than 30% by weight of the total cholesterol content when ingested in the human body The method according to claim 1, wherein the silver nano-coated inorganic salts contain 0.01 to 0.5 parts by weight of silver nano compounds based on 1 part by weight of one or more inorganic salts selected from sodium chloride, calcium chloride, potassium chloride, magnesium chloride, sodium sulfate, and the like. Functional egg According to claim 1, wherein the silver nano-coated inorganic salt is a healthy functional egg, characterized in that prepared by the vapor deposition of silver nano particles of particle size of 1-50 nm in inorganic salts