KR20050110719A - Cosmetic composition for moisturizing effect on the skin - Google Patents

Abstract

The present invention relates to a cosmetic composition for skin moisturizing containing Portulaca oleracea extract, natto gum, hyaluronic acid, and honey extract. More specifically, the cosmetic composition for moisturizing the skin of the present invention contains Portulaca oleracea extract, natto gum, hyaluronic acid, and honey extract, and skin pulling and roughness commonly occurring in women. Improves water shortage.

Description

Cosmetic composition for skin moisturizing {Cosmetic composition for moisturizing effect on the skin}

The present invention relates to a cosmetic composition for skin moisturizing containing Portulaca oleracea extract, natto gum, hyaluronic acid, and honey extract. More specifically, the cosmetic composition for moisturizing the skin of the present invention contains Portulaca oleracea extract, natto gum, hyaluronic acid, and honey extract, and skin pulling and roughness commonly occurring in women. Improves water shortage.

The skin is divided into three layers of epidermis, dermis and subcutaneous tissue in order from the outside, and it protects the organs of the body from temperature and humidity changes, ultraviolet rays and other physical and chemical external stimuli. In particular, the epidermis plays an important role in preventing evaporation of moisture inside the human body.

The epidermis is divided into the stratum corneum, granule layer, pore layer, and basal layer in order from the outside, the cells of the stratum corneum act like a brick and the intercellular lipids between the stratum corneum act as a mortar to form a skin barrier ( J. Invest.Drmatol. 80 (Suppl.), 44-49. 1983. In addition, the keratinocytes of healthy people have a high concentration of Natural Moisturing Factor (NMF) to help the skin retain moisture. For example, because amino acids, such as amino acids, are water soluble, they effectively combine with moisture Inhibit drying (J. Invest. Dermatol. 54, 24-31, 1970).

However, these days, the artificial temperature control of air conditioning and heating according to the change of environment and life patterns, the skin stress caused by various stresses and environmental pollution that occur in social life, the frequent face wash due to makeup habits and the increase of age The need for skin moisturizers is increasing because the moisture in the stratum corneum decreases due to various causes such as normal skin aging, and the skin becomes dry, the surface is rough, the skin becomes crumbly and moisturized, and it appears to be lifeless. .

Conventionally, a method of increasing moisture retention in the stratum corneum by using a humectant having a property of absorbing moisture or an occlusive moisturizer that prevents evaporation of moisture as a moisturizing agent is used. Examples of the humectant include glycerin, propylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, and 2-pyrrolidone-5-sodium carboxylate, but are very sticky or damp when applied to the skin. There is a drawback to feeling. In addition, as a closed moisturizer, lipid components such as ceramide, essential fatty acids and lipid complexes were used (J. Invest. Dermatol. (5), 731-740, 1994), but it is difficult to maintain the stability of the emulsion formulation and is transparent. There are disadvantages in inadequate preparation of gelled products.

However, in recent years, the claim that differentiation of the stratum corneum is important to prevent drying of the skin moisture, and the differentiation process that develops from the basal keratinocytes to the outermost stratum corneum cells should be performed normally to act as a skin moisture barrier. It turns out that you can. That is, during the process of keratinization, the cells produce natural moisturizing factors and intercellular lipids, and as a result, the stratum corneum becomes firm and flexible, thus functioning as a barrier. Physiologically, the tendency of the skin to dry with age increases, which can be interpreted as increasing the dropout time of the stratum corneum, decreasing the lipid synthesis ability of epidermal cells, and decreasing the amount of moisturizing factors and lipids in the stratum corneum. . Thus, by accelerating the differentiation of keratinocytes, a new approach has been made that can induce a strengthening of the skin barrier and enhance skin moisture retention and protection from the external environment.

Thus, the present inventors have studied a cosmetic composition excellent in the moisturizing effect of the skin to improve the skin pulling, roughness, lack of moisture, but excellent in the stability of the formulation without sticky or sloppy when applied to the skin, gusset extract, natto gum, The cosmetics containing hyaluronic acid and honey extract were prepared and confirmed to have excellent skin moisturizing effect when applied to human skin. This moisturizing effect promotes differentiation of keratinocytes, thereby enhancing the skin barrier. The present invention has been completed by revealing that it was induced to improve skin moisture retention function.

Therefore, an object of the present invention is to provide an excellent moisturizing effect on the skin by containing Machihyeon extract, natto gum, hyaluronic acid, and honey extract as an active ingredient for the skin moisturizing can improve the pulling, roughness, lack of moisture phenomenon of the skin It is to provide a cosmetic composition.

In order to achieve the above object, the skin moisturizing cosmetic composition according to the present invention is characterized in that it contains an extract, natto gum, hyaluronic acid, honey extract (Honey extract).

More preferably, the cosmetic composition for moisturizing the skin according to the present invention is 0.001-20% by weight of Machiko extract, 0.00001-10% by weight of natto gum, 0.00001-10% by weight of hyaluronic acid, and 0.00001-10% of honey extract. It is characterized by containing a weight%.

Hereinafter, the present invention will be described in more detail.

Portulaca oleracea extract used in the present invention is extracted from the leaves, stems, roots of Portulaca oleracea l -norepinephrine, vitamins A, B1, B2, C, α- tocopherol, β- carotene, omega-3 fatty acids, glutathione, It contains glutamic acid, malic acid, citric acid and the like, and has been used for various skin diseases since it has antibacterial, anti-inflammatory, wound healing, and anti-inflammatory effects on the skin.

In the present invention, the content of the extract of Machi Prefecture is preferably 0.001 to 20% by weight, less than 0.001% by weight of the effect on the skin is weak, and more than 20% by weight is not particularly suitable for cosmetics.

On the other hand, natto gum used in the present invention is a natural polymer component obtained by culturing microorganisms in soybean, and glutamic acid and fructose are the main components, and excellent raw material with excellent moisture retention and lubricity.

Natto gum content in the present invention is preferably 0.00001 ~ 10% by weight, less than 0.00001% by weight is less effective for the skin, more than 10% by weight is difficult to completely dissolve in water and sticky feeling is suitable as a cosmetic Not.

Meanwhile, hyaluronic acid used in the present invention is a natural polymer obtained by culturing microorganisms and is in the form of free acid or salt. In addition, the hyaluronic acid is a kind of mucopolysaccharide is present in the connective tissue of the human dermal layer is a component that plays an important role in water retention.

In the present invention, the content of hyaluronic acid is preferably 0.00001 to 10% by weight, less than 0.00001% by weight is less effective for the skin, more than 10% by weight it is difficult to completely dissolve in water, because the sticky feeling appears as a cosmetic Inappropriate.

On the other hand, the honey extract used in the present invention (Honey extract) is a material extracted from honey contains oligosaccharides (oligosaccharide), amino acids (amino acid), a large amount of trace elements. In particular, it has a biochemical composition similar to that of natural moisturizing factor (NMF), and shows excellent function in controlling skin moisture. In addition, it has excellent ability to retain bound water and moisture absorbed from the outside, so it maintains moist skin condition for a long time. It acts specially on the stratum corneum of the epidermis to adjust the balance of moisture, give long-term moisturizing effect and dehydration phenomenon. Delay.

In the present invention, the trade name Melhydran (manufacturer: Laboratoire Serobiologiques) was used as the honey extract, and the content of the honey extract is preferably 0.00001 to 10% by weight based on the total weight of the composition. It is less than 0.00001% by weight of the effect on the skin is weak, more than 10% by weight is difficult to dissolve completely in water and is not suitable as a cosmetic because of the sticky feeling.

Hereinafter, although an Example and a test example are given and this invention is demonstrated more concretely, this invention is not limited only to these examples.

[Test Example 1] keratinocyte differentiation promoting effect

Human keratinocytes were placed in a culture flask, cultured, and grown to about 80%, and then the samples of Table 1 were each added at a concentration of 5 ppm and treated for 4 days. Next, a denatured protein such as urea or sodium dodecyl sulfate (SDS) and a reducing agent such as β-mercaptoethanol were mixed to remove the dissolved protein. Then, the peptide concentration was analyzed by measuring the absorbance at 310 nm of the remaining stratum corneum, and the results are shown in Table 1 by comparing the increase by each sample based on distilled water as a negative control (0%).

matter Keratinocyte differentiation promoting effect March 73% Natto gum 45% Hyaluronic acid 27% Melhydran (Honey extract) 35% Distilled Water (Negative Control) 0%

From the results of Table 1, it can be seen that the keratinocyte differentiation promoting effect of the materials used in the present invention is very excellent.

[Examples 1-3 and Comparative Examples 1-3] Preparation of Cosmetics for Skin Moisturizing

The aqueous parts up to components 1 to 8 and the alcohol parts up to components 9 to 12 were dissolved at room temperature, respectively, according to the weight percents shown in Table 2, and then the alcohol parts were gradually added to the aqueous phase while solubilizing with stirring. Next, after the addition of the alcohol part was completed, stirring was continued for about 3-5 minutes, and then vacuum degassing to complete the preparation of the cosmetic.

Ingredients (content; weight percent) Comparative Example 1 Comparative Example 2 Comparative Example 3 Example 1 Example 2 Example 3 1.Purified water to100 to100 to100 to100 to100 to100 2.glycerine 3 3 3 3 3 3 3.Carbomer 0.2 0.2 0.2 0.2 0.2 0.2 4.triethanolamine 0.18 0.18 0.18 0.18 0.18 0.18 5.Machi Prefecture Extract - - One One 2 2 6.Natto gum - 0.2 - 0.2 0.2 0.4 7.hyaluronic acid - 0.1 - 0.1 0.1 0.2 8.Melhydran - 0.1 - 0.1 0.1 0.2 9.ethanol 3 3 3 3 3 3 10.Polyoxyethylene Hydrogenated Castor Oil 0.3 0.3 0.3 0.3 0.3 0.3 11. Incense Quantity Quantity Quantity Quantity Quantity Quantity 12. Preservative Quantity Quantity Quantity Quantity Quantity Quantity

Test Example 2 Skin Barrier Function Restoration Effect

After repeatedly applying acetone to the skin of hairless mice, the barrier function was impaired. The epidermal water loss (TEWL) was measured by servomed evaporimeter EP1 and measured at 4.0 g / ㎡ / h. When reached, the sample containing the composition of Table 2 was applied to a 5 cm 2 area. Next, the extent of barrier function recovery was evaluated by evaluating the extent of epidermal moisture loss after 1 hour, 2 hours, 4 hours, and 8 hours. At this time, a known lipid mixture (2: 1: 1 mixture of ceramide: cholesterol: fatty acid) was used as a positive control. The results are shown in Table 3 below comparing the changes over time based on the amount of epidermal moisture loss in the initial damaged barrier state (100%).

Epidermal moisture loss (%) sample 1 hour later 2 hours later 4 hours later 8 hours later Comparative Example 1 88 90 77 75 Comparative Example 2 66 54 48 39 Comparative Example 3 78 62 50 36 Example 1 63 54 41 27 Example 2 58 52 36 24 Example 3 51 44 37 23 Geological Mixture (Yangseong Control Group) 63 45 39 28

From the results of Table 3, it was confirmed that the skin barrier function recovery effect of Examples 1 to 3 according to the present invention is very excellent compared to Comparative Examples 1 to 3.

[Test Example 3] skin moisture content increase effect

Experimental effect of improving the skin moisturizing power for the cosmetic prepared in the composition of Table 2. First, divided into seven sets of 20 people per group for 140 people in their 20s to 30s without skin diseases, and each group of Examples 1 to 3 and Comparative Examples 1 to 3 and a positive control group as shown in Test Example 2, respectively. It was applied twice a day for 1 month to face and forearm. Before starting the application, measure the conductivity of the skin using a corneometer (CM820 courage Khazaka electronic GmbH, Germany) at constant temperature and humidity conditions (24 degrees, 40% humidity), and after 1 week, 2 weeks and 4 weeks And skin conductivity two weeks after application discontinuation (6 weeks) and the rate of increase was evaluated. The results are shown in Table 4 below.

Skin conductivity increase rate (%) sample 1 week later after 2 weeks 4 weeks later 6 weeks later Comparative Example 1 10 13 16 8 Comparative Example 2 41 43 48 24 Comparative Example 3 43 46 50 36 Example 1 57 62 59 42 Example 2 58 61 67 55 Example 3 60 67 73 61 Geological Mixture (Yangseong Control Group) 63 65 64 44

Since the skin conductivity is proportional to the skin moisture content from the results of Table 4, it was confirmed that the skin moisture content increase effect of Examples 1 to 3 according to the present invention is very excellent in the above results. In addition, in the case of Examples 2 and 3, even after two weeks of discontinuation (after six weeks in total), the moisture content was maintained higher than other examples, and thus, it was found to have a long-term moisturizing effect.

[Test Example 4]

The subjects of Test Example 3 were subjected to subjective efficacy evaluation through a questionnaire on skin pulling, roughness, and lack of moisture after using Examples 1-3 and Comparative Examples 1-3. The questionnaire survey was to indicate the satisfaction of the product for each item of skin pulling, roughness, lack of moisture, divided by 1-5 points (the higher the number, the higher the satisfaction), and the results are shown in Table 5 below.

sample Satisfaction (Average Score) Skin Pulling Improvement Skin Roughness Improvement Improvement of skin moisture deficiency Comparative Example 1 1.8 1.4 2.1 Comparative Example 2 1.3 2.2 2.3 Comparative Example 3 2.7 3.5 2.6 Example 1 3.5 3.7 3.2 Example 2 4.2 4.7 3.9 Example 3 4.0 4.8 4.1

From the results of Table 5, it can be seen that in the case of Examples 1 to 3 according to the present invention, the effect of improving the skin pulling, roughness, and lack of moisture was superior to Comparative Examples 1 to 3.

As described above, the cosmetic composition containing Machi Prefecture extract, natto gum, hyaluronic acid and honey extract provided by the present invention promotes the differentiation of keratinocytes of the skin and restores the skin barrier function, pulling, roughness of the skin. It has the effect of eliminating and increasing the luster can be usefully used as a cosmetic for moisturizing the skin.

Claims (2)

A cosmetic composition for skin moisturizing, comprising as an active ingredient Machi Prefecture extract, natto com, hyaluronic acid and honey extract. The method according to claim 1, wherein the active ingredient contains 0.001-20% by weight of Machiko extract, 0.00001-10% by weight of natto gum, 0.00001-10% by weight of hyaluronic acid, and 0.00001-10% by weight of honey extract. Cosmetic composition for skin moisturizing, characterized in that.