KR20050102761A - Concentrated mixture of plant extracts having insecticidal activity - Google Patents

Abstract

The present invention relates to a concentrated plant extract mixture having pesticidal activity.

In the method for preparing a plant extract concentrated mixture having insecticidal activity according to the present invention, the extract is concentrated with alcohol, Ginseng, red ginseng, branch vein and night country, and the extract concentrate 1 is prepared, and the extract of hot water is extracted from Camellia, Jinrang, Sagunja, white paper, and Yeo. Then, the extract concentrate 2 is prepared by concentrating, and then, each of the prepared extract concentrate 1 and extract concentrate 2 is mixed in the same ratio to prepare a plant extract concentrate mixture.

According to the present invention, there is provided a plant extract concentrated mixture having a specific pesticidal activity against various pests harmful to crops, and a method for producing a plant extract concentrated mixture having an insecticidal effect is provided.

Description

Concentrated MIXTURE OF PLANT EXTRACTS HAVING INSECTICIDAL ACTIVITY

The present invention relates to a concentrated plant extract mixture having pesticidal activity.

The use of pesticides and pesticides became active with the development of DDT by Mueller in 1939.

Although these chemical pesticides had a great effect at the beginning, they were found to be accumulated in animals and human bodies as their usage increased, and the boomerang effect was found to be increasingly damaging the environment and the food chain.

That is, the conventional chemical pesticides have a problem that 85 ~ 90% of the pesticides containing pesticides are scattered in the air, soil, and water, which continuously destroys the environment, becomes more resistant and resistant to pests, and harms the human body. .

Therefore, in recent years, various efforts have been made to solve residual pesticides and environmental pollution without any harm to the human body such as various microbial agents, breeding of varieties resistant to diseases and pests, and development of natural pesticides.

In Korean Patent Application Publication No. 2002-0068021 (Herbal extract fertilizer for plant disease control and its manufacturing method), Cheonmun-dong, Betel nut, Baekeun-geun, Cheonnyeonja, Clove, Health, Milk, Sagunja, Jimo, Daegye, Hakseul, Hwangbaekpi, Casualties For plant disease control, extracting wood, yeast, red pepper, young leaf, quince, black porridge, pogongyoung, kyokyo, gold and silver, erosion, dry chile, erectile, edible leaf Chinese medicine extract fertilizer is open to the public.

Korean Unexamined Patent Publication No. 2003-0068255 (Plants' extract-containing plant powdery mildew control composition) discloses a composition obtained by extracting a squirrel or a squirrel with an organic solvent, and concentrating the extract under reduced pressure.

Korean Patent Application Publication No. 1993-0009511 (Agricultural pest control agent) is characterized in that the stem and leaves of ginkgo biloba, stems and leaves of ginkgo biloba consists of water or extracts of water and alcohol and fine powder of stems and leaves of motherwort. It is disclosed about crop pest control agent to be said.

However, fertilizers and pest control agents using the conventional plant extracts as described above do not have a large insecticidal activity, which has a problem in that the effect is inferior.

In order to achieve the above object, it is an object of the present invention to provide a concentrated plant extract mixture having a specific pesticidal activity against a variety of pests harmful to crops and a method for producing the same.

The present invention relates to a concentrated plant extract mixture having pesticidal activity.

In the method for preparing a plant extract concentrated mixture having insecticidal activity according to the present invention, the extract is concentrated with alcohol, Ginseng, red ginseng, branch vein and night country, and the extract concentrate 1 is prepared, and the extract of hot water is extracted from Camellia, Jinrang, Sagunja, white paper, and Yeo. Then, the extract concentrate 2 is prepared by concentrating, and then, each of the prepared extract concentrate 1 and extract concentrate 2 is mixed in the same ratio to prepare a plant extract concentrate mixture.

Referring to the production method of the concentrated plant extract mixture of the present invention in more detail as follows.

In the method for preparing a concentrated plant extract having a pesticidal activity according to the present invention, grinding pulverulum, red ginseng, bran and wild gland, respectively, put 2 l of 75% ethyl alcohol per 1 kg of pulverized powder, and extract for 4 hours. The alcohol extract is heated to 90 ~ 100 ℃ 6 concentration to prepare an extract concentrate 1, respectively.

Camellia, Jinrang, Sagunja, White Paper, Yeo-ro, 10 liters of water per kilogram each, heated to 90-100 ℃ for 24 hours, and extracted with hot water. Concentrate while heating to 90 ~ 100 ℃, then filtered to prepare extract concentrate 2, respectively.

Each of the prepared extract concentrate 1 and extract concentrate 2 were mixed at the same ratio, 30% ethyl alcohol was added, the concentration and volume were adjusted, and the mixture was filtered to prepare a plant extract concentrate.

The inventors of the present invention, in order to develop a novel microbial pesticide with excellent biological control ability, nine kinds of pesticides and mortality of nine pesticides and extracts of medicinal plants, showing significant insecticidal effect Plants were selected.

Plants used in the preparation of the plant extract concentrated mixture of the present invention are, ginseng, red ginseng, branch vein, night country, camellia, Jinrang, Sagunja, white paper, young.

The inventors of the present invention went through a lot of trial and error to find a method that shows the most insecticidal effect using the above nine selected plants, and at the end of the study, Ginseng, red ginseng, branch vein, and yam were concentrated after alcohol extraction. Was found to have the highest pesticidal activity, and camellia, jinlang, monsoon, white paper, and distilled hydrothermal extract followed by alcohol extraction and concentrating the most.

The plant extract concentrated mixture of the present invention thus invented may be used in a liquid state, and may be used after being dried and powdered, and diluted to 400 to 1,200 times when used.

Plant extract concentrated mixture of the present invention is a pest of crops (BPH: Brown PlantHopper), Spotted Mung (TSSM: Two Spotted SpiderMite), Tobacco Cutworm (TCW: Tobacco CutWorm), Blueworm, Oyster, Aphid, Pear Tree, Green onion It has a specific insecticidal activity spectrum such as chestnut moth, carnivorous moth, whiskers, greenhouse dust, slug, aphid, rice weevil, pearworm moth, or moth moth. It can be usefully used as a functional environmentally friendly pesticide.

Plant extract concentrated mixture having an insecticidal effect of the present invention can be used in soil improving agent, composting agent, foliar spray, irrigation spray.

On the other hand, a brief description of the plant material used in the preparation of the concentrated plant extract mixture having pesticidal activity of the present invention.

Celiac refers to the bark of Picrasma quassioides Benn, which is known to have an anti-inflammatory and anti-inflammatory effect.

Ginseng is a legume, has high anti-allergic activity and is known to have diuretic, uterine contraction, antipyretic, analgesic and insecticidal effects.

Yak-guk is the abbreviation of wild chrysanthemum, it is known to have the effect of relieving poison, effective in swelling and changchang, and reducing the heat of the liver.

It is known that it is effective as a blood donor, hemostatic agent, nourishment, and tonic.

Camellia protects the liver and kidneys, strengthens muscles and bones, eliminates customs, relieves cramps and numbness, causes meridians, and is effective in treating individual, high blood pressure, nephritis, diabetes, cancer, gastrointestinal diseases, epilepsy, and nervous breakdowns. Known.

Betel nut is a palm tree and fruit, betel fruit is known to be effective in removing the tapeworms, constipation, edema, headache, and to increase the resistance to the epidemic virus, inhibit the skin fungi.

The sand stalk is a vine plant of the creeper family, and is known to be effective in roundworms, skin diseases, gastrointestinal diseases, and the like.

White paper is a plant of the genus Amphibori, which is called copper leaf. The root and stem of white paper are used for medicines such as cold, blood, pain, soothing, Jinhae, anemia, gynecological diseases, headache, diuresis, weight lifting, epilepsy, health, morale, toothache, pain and pain. It is known to have an antibacterial effect.

Yero is a perennial herb belonging to the family Liliaceae. It has been shown to lower blood pressure, hepatoprotective and bile secretion. It is effective in killing parasites in the stomach, and bathing cows, horses, dogs, etc. It is known to kill all insects such as ticks and fleas that are parasitic on the skin.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples, but these do not limit the scope of the present invention.

Example 1 Preparation of Plant Extract Concentrated Mixture Having Insecticidal Activity

On the market, they prepared Goren, Ginseng, Baekbaek, Noodles, Camellia, Betel Lang, Sagunja, White Paper, and Jeolla.

Cinnamon (root), red ginseng (root, stem), cypress (leaves, stem) and wild ginseng (root, stem) are ground and soaked in 2 kg of 75% Et-OH. Extraction was added with shaking for 4 hours.

The alcohol extract was filtered, and then concentrated six times while heating to 90 ° C., thereby preparing an extract concentrate 1.

Camellia (flowers, leaves), betel nuts (roots), sandals (roots), white paper (shells), and paths (roots) are not crushed, and 1 kg each of 10 l of water is heated and heated to 90 ° C for 24 hours. Extracted.

6 L of 75% Et-OH was added to 1 L of the hot water extract, heated to 90 ° C. for 6 hours, concentrated, and filtered to prepare extract concentrate 2.

The prepared extract concentrate 1 and the extract concentrate 2 were mixed in the same ratio, and then filtered by adjusting the volume with 30% Et-OH to prepare a plant extract concentrate mixture showing the final pesticidal activity.

Comparative Example 1 Comparative Preparation of Extracted Mixture

A material was prepared as in Example 1.

Cinnamon, red ginseng, baekbaek, and ginseng were pulverized, and then, 1 kg of each pulverized product was added with 2 L of 75% Et-OH to immerse, shaken for 4 hours, and then filtered.

Camellia, betel nut, four sands, white paper, yeoseo without grinding, put each 1 kg in 10 l of water and heated to 90 ℃ for 24 hours to extract the hot water.

6 L of 75% Et-OH was added to 1 L of the hot water extract, followed by extraction for 6 hours, followed by filtration.

Each of the prepared extracts was mixed at the same ratio to prepare an extract mixture.

Experimental Example 1 Activity Analysis of Plant Extract Concentrated Mixture Having Insecticidal Activity

The plant extract concentrated mixture of Example 1 of the present invention and the extract mixture of Comparative Example 1 were prepared.

Insecticidal activity analysis was commissioned to the chemical research institute as well as in-house analysis and analyzed in the following way, the analysis results are shown in Table 3.

1.Spray method

1) Spotted Mite: Approximately 30 adults were placed next to 3cm diameter kidney beans, sprayed with certain chemicals in the hood, and shaded after 24h and 48h.

2) Rice hoppers: 5 seedlings of rice seedlings were rolled on cotton wool and placed in a test tube, followed by 20 insects in 3 insects, sprayed with medicinal solution in the hood, and shaded after 24h and 48h.

3) Aphids, blue worms, oyster flies, pear trees, carnivorous moths, green chestnut moths, whiskers, and greenhouse dusts were applied directly to the developmental packaging and observed for insecticidal effects.

2. Leaf dipping method

1) Tobacco gerberabang: 5cm diameter cabbage leaves were immersed in the prepared medicinal solution for about 30 seconds, then shaded in the hood and inoculated with 10 three-larvae larvae of tobacco gerberabang and examined for insecticidal rate after 24h and 48h.

2) Chinese cabbage leaf moth: 5cm diameter cabbage leaf was immersed in the prepared drug solution for about 30 seconds, then shaded in the hood and inoculated with 10 larvae of Chinese cabbage leaf moth 3 larvae were examined after 24h, 48h.

3) Peach aphids: 5.5cm diameter round tobacco leaves were immersed in the prepared medicinal solution for about 30 seconds, then shaded in the hood and inoculated with 10 adult peach aphids at 24h and 48h.

<Table 3> Insecticide Activity Survey Results

division Comparative Example 1 Example 1 Brown Planthopper 24 h 100 100 48 h 100 100 Diamondback Moth 24 h 10 100 48 h 30 100 Two Spotted Spider Mite 24 h 0 100 48 h 0 100 Tobacco Cutworm 24 h 0 50 48 h 20 100 Whisker 24 h 100 100 48 h 100 100 Gullfly 24 h 100 100 48 h 100 100

* The numbers indicate Mortality (%).

-100 means 100% pesticide rate, and 0 indicates no efficacy.

3. Results of Insecticidal Activity

As shown in Table 3, the insecticidal activity plant extract concentrated mixture of Example 1 was superior to the insecticidal spectrum as well as the insecticidal effect than the extract mixture of Comparative Example 1.

That is, in Comparative Example 1 using the non-concentrated extract mixture, there was almost no insecticidal effect on Chinese cabbage moth, spotted mite, tobacco germweed moth, and especially when using the plant extract concentrated mixture of Example 1 of the present invention. Insect control effect is also enhanced in tobacco spider moths, which are very difficult to control.

In addition, insect repellent tests for the nymphs, aphids, blueworms, oyster flies, greenhouse powder, nectarine, green chestnut moth, and deep moth were 100 in 24 hours in the group using Example 1 of the present invention when applied directly by spraying in development packages. The insecticidal effect of% was shown.

In addition, it was found that there was no harm to bees, natural insects and human beings.

According to the present invention, there is provided a plant extract concentrated mixture having a specific pesticidal activity against various pests that cause harm to crops, and a method for producing the same.

1 is a manufacturing process of the plant extract concentrated mixture having pesticidal activity of the present invention.

Claims (6)

In the method of producing a plant extract having plant activity, Prepare the extract concentrate 1 by extracting alcohol and extracting Ginseng, red ginseng, side vein, and night country, Hot water extract camellia, Jinrang, Sagunja, white paper, and Jeolla, and concentrated to prepare extract concentrate 2, Comprising a mixture of each of the prepared extract concentrate 1 and extract concentrate 2 in the same ratio to produce a plant extract concentrated mixture, Method for producing a concentrated plant extract mixture having pesticidal activity. The method of claim 1, In the preparation of the extract concentrate 1, grits, red ginseng, branch vein, and wild gland were respectively ground, followed by adding 2 l of 75% ethyl alcohol per kg of ground powder, extracting for 4 hours, and then heating the alcohol extract to 90 to 100 ° C. To concentrate 6 times to prepare an extract concentrate 1, characterized in that Method for producing a concentrated plant extract mixture having pesticidal activity. The method of claim 1, In the preparation of extract concentrate 2, 10 liters of water per 1 kg of camellia, jinlang, Sagunja, white paper, and yeast were added and heated to 90-100 ° C for 24 hours, followed by 75% ethyl alcohol per 1 liter of this hot water extract. 6 L was added and concentrated while heating to 90-100 ° C. for 6 hours, followed by filtration to prepare extract concentrate 2, respectively. Method for producing a concentrated plant extract mixture having pesticidal activity. The method of claim 1, When the extract concentrates are mixed, the extract concentrates 1 and extract concentrates 2 are mixed at the same ratio, and then 30% ethyl alcohol is added and the concentration and volume are adjusted, followed by filtration to prepare a plant extract concentrate mixture. Method for producing a concentrated plant extract mixture having pesticidal activity. Prepared by the method of any one of claims 1 to 4, Concentrated plant extract mixture with insecticidal activity. Using the plant extract concentrated mixture prepared by the method of any one of claims 1 to 4, using rice plant, cabbage moth, spotted mite, tobacco gerbera moth, whiskers, oyster fly, greenhouse powder, blue worm, green chestnut moth, A method of controlling one or more pests selected from carnivorous moths, slugs, aphids, rice weevils, Ewha moths and dead moths.