KR20050090648A - The manufacturing method of natural tea having eliminatory effect of ethanol-induced hangover and containing mixed extract of gastrodia elata and hovenia dulcis thunb & its product - Google Patents

Abstract

The present invention reveals that there is a hangover removal effect on the cheonma, and by combining the natural substances such as earth tree (heaven tree) fruit in the appropriate ratio in order to provide a natural tea excellent in hangover elimination effect, synthetic drugs as raw materials It aims to ensure the safety that the hangover elimination drink cannot have and at the same time to allow the person who drinks it to lower the blood alcohol level and alleviate or eliminate the hangover symptoms caused by drinking.

Chopped and mixed with 7.5% of Cheonma, 35.0% of Raven (earth), 10.0% of Jujube, 10.0% of Duck, 10.0% of Brown Root, 9.0% of Cornus, 5.0% of Schisandra chinensis, 5.0% of Schisandra chinensis, 4.0% of Mint, and Elm 2.0%;

Adding 10 times purified water to the mixture above the total weight of the mixture and performing cold extraction at 85-90 ° C. for 6 hours;

Filtering the extract above to cool;

Adding the following additional substances to the extract, the extract 80%, liquid fructose 12.0617%, fragrance 0.1200%, stevione 0.0050%, citric acid 0.0044%, purified water 7.8089%; hangover It is characterized in that it is a natural tea produced by the manufacturing method and natural tea.

Description

The manufacturing method of natural tea having eliminatory effect of Ethanol-Induced Hangover and containing mixed extract of Gastrodia elata and Hovenia dulcis Thunb & its product}

The present invention relates to a natural tea with excellent hangover effect and a method of manufacturing the same. More specifically, the traditional natural materials such as extracts of cheon-ma and earth tree fruit as the main raw material, excellent body safety and hangover compared to similar products The present invention relates to a natural hangover tea excellent in effect and a method of manufacturing the same.

Korea is the country that drinks the most among the 30 member countries of the Organization for Economic Cooperation and Development (OECD). In particular, the consumption of solo wine such as soju is 5.6 times higher than the average consumption of 29 OECD countries except Korea, and the annual economic and social losses caused by drinking are estimated to be trillion won. The main cause of hangover symptoms as a result of this drinking culture is acetaldehyde, which is produced during the decomposition of alcohol.As the amount of acetaldehyde in the blood increases, blood pressure decreases and blood circulation to the brain worsens, causing headaches. It is known to cause nausea, vomiting, dizziness, thirst due to dehydration, diarrhea, hormone modulation and muscle pain.

Ethanol, a major component of alcohol, is rapidly absorbed by the gastrointestinal tract and is metabolized mainly by the liver. In the liver, there are three different enzyme systems involved in ethanol metabolism, such as alcohol dehydrogenase (ADH) pathway, microsomal ethanol-oxidizing system, and catalase. It is known that the ADH pathway mainly works under normal conditions. Normally consumed ethanol is metabolized to acetaldehyde by cytosolic ADH, which in turn is oxidized to acetate by mitochondrial aldehyde dehydrogenase (ALDH).

Drugs that can quickly and safely lower the concentration of ethanol in the blood after ethanol intake can alleviate the hangover caused by ethanol. In general, it has been reported that ethanol ingested orally lowers blood levels by two mechanisms that promote metabolism in vivo or inhibit absorption through gastrointestinal system. Recently, in response to the negative social trend of drinking culture and the growing interest in the development of new drugs, some medicines have been developed to reduce or eliminate hangovers caused by drinking, but these drugs show their own toxicity and side effects, which is a safer natural product. Much attention has been paid to the development of health drinks using.

It is an object of the present invention to find out that there is a hangover removal effect on Gastrodia elata Blume , and to provide a natural tea excellent in hangover effect by combining pure natural substances such as earth tree (hazelnut) fruit in such a ratio. This ensures the safety of the hangover elimination drink, which uses synthetic drugs as a raw material, and allows people who drink it to lower blood alcohol levels and alleviate or eliminate hangover symptoms caused by drinking. have.

The present inventors have combined and processed various kinds of natural extracts, which are expected to have a hangover removal effect or fatigue reduction effect, in various compositions, through the literature and the like. I confirmed it.

The present invention for achieving the above object,

Chopped and mixed with 7.5% of Cheonma, 35.0% of Raven (earth), 10.0% of Jujube, 10.0% of Duck, 10.0% of Brown Root, 9.0% of Cornus, 5.0% of Schisandra chinensis, 5.0% of Schisandra chinensis, 4.0% of Mint, and Elm 2.0%;

Adding 10 times purified water to the mixture above the total weight of the mixture and performing cold extraction at 85-90 ° C. for 6 hours;

Filtering the extract above to cool;

Adding the following additional substances to the extract, the extract 80%, liquid fructose 12.0617%, fragrance 0.1200%, stevione 0.0050%, citric acid 0.0044%, purified water 7.8089%; hangover It is characterized in that it is a natural tea produced by the manufacturing method and natural tea.

Gastrodia elata Blume is an underground tuber of perennial herb belonging to the Orchidaceae family, and its effects are found in the new farms, medicinal herbs , forest economy, Dongbogam, and medicinal herbs. It has a wide range of efficacies such as removing phlegm and moist, treating winds, relieving inflammation, increasing fluids, stopping pinching, stopping diarrhea, releasing poisons, relieving various weaknesses, and relieving pain. It is also known as an herbal medicine that is also effective in diabetic blood pressure, headache and diabetes. Chunma has been traded at high prices in Chinese medicine until recently, but recently, artificial cultivation technology has been developed to allow mass production. In the present invention, it was confirmed that the hangover removal effect is excellent by adding the cheonmama to the main components.

Hovenia dulcis Thunb, the main composition of the hangover natural tea according to the present invention is the hangover removal, thirst quenching, treatment of fecal irritation by alcohol poisoning, muscle relaxation caused by alcohol It has been reported to have effects such as alcohol metabolism and liver protection effect of alcohol. This ingredient is included with Cheonma to maximize hangover relief.

Alnus (Alnus japonica) is that the efficacy and colitis, and diarrhea, are used for treatment of trauma bleeding and bloody stool, Puerariae Radix (Puerariae radix) has antioxidant activity, fatty peroxidation inhibitory action and the interpolation function, such as fever, bleeding, convergence Known.

In addition, jujube, licorice, cornus oil, Schisandra chinensis, peppermint, and elm were mixed at a certain ratio, and liquid fructose, flavor, stevion, and citric acid were added. Could. Liquid fructose and flavorings are generally commercialized and can be easily obtained and used. In some cases, they can be used depending on the desired flavor within the range.

In addition, the materials used in the present invention are all items approved by the Food and Drug Administration Food Code.

Hereinafter, the experimental example of the present invention.

The inventors objectively verified the efficacy of the hangover removal natural tea according to the present invention based on scientific data. As a method, the pharmacokinetics of the change of blood ethanol concentration over time in experimental animals in vivo From the viewpoint of phosphorus, the efficacy of the hangover removal of the present invention was examined, and the safety of the product and the mechanism of change in blood ethanol concentration were observed through changes in the activity of the indicator enzyme and ethanol metabolizing enzyme of liver function. In addition, by conducting a comparative experiment with the efficacy of some products currently in circulation and on the market to confirm the superiority of the hangover removal effect of the present invention.

1. Preparation of natural tea for hangover

The natural tea for hangover used in this experiment was produced from Hokkaia dulcis Thunb, Gastrodia elata , Alnus japonica , and Root ( Cheonbuk Natural Food). A total of 16 natural items, including Puerariae radix ) and peppermint ( Mentha arvensis ), were prepared as raw materials.

First, as shown in the table below, the selected 10 materials were chopped and mixed at a predetermined ratio, and 10 times of purified water was added to the total mixture of the 10 materials, followed by low temperature extraction at 85 to 90 ° C. for 6 hours. The extract was filtered and cooled at room temperature, and liquid fructose, flavor, stevione, citric acid and purified water were added thereto, but 80% gastric extract, 12.0617% concentrated fruit juice, liquid fructose, 0.1200% flavor, 0.0050% stevia, Finally, a natural tea for hangover resolution was prepared by using a ratio of 0.0044% citric acid and 7.8089% purified water, which was used as a sample in this experiment.

2. Experimental Animals and Treatment

Animals were purchased from Daewoo Biolink (negative voice) of Daewoo Sprague-Dawley rats, weighing 220 ± 20 g, 6 weeks of age, at constant conditions (temperature: 22 ± 2 ° C, humidity: 55 ± 5%, Contrast: 12 hours light / dark cycle) was used after 5 days of adaptation, the experimental group was normal, control group, product administration group according to the present invention (tentative name 'Siwoncheong') and two other companies It was divided into five groups of families (P1 and P2).

First, the ethanol was fasted for 12 hours before oral administration of ethanol to eliminate ethanol obstruction through the gastrointestinal tract, and the hangover remover was administered orally 2 mL 30 minutes before ethanol administration (20 ), Ethanol was administered orally at a level of 3 g / kg body weight of 25% ethanol according to the method of Fujii et al. (25). The control group was orally administered with 2 mL of tap water instead of the sample, and the normal group gave no conditions.

To measure blood ethanol concentration over time, in group 4 except normal group, ethanol was administered orally over time (0.25, 0.5, 1, 2, 4, 6, 8, 10, and 12 hours). Blood collection was performed through the aorta (200 μL). Blood obtained over time was rapidly centrifuged at 4 ℃, 3,000 rpm for 15 minutes to obtain a serum, immediately stored in a -80 ℃ cryogenic freezer and frozen. The feed [Samyang Oil Feed Co., Ltd., Wonju, Gangwon-do] was fed again from 4 hours to 12 hours before blood collection and then fasted for 12 hours before the animals were treated. Water was supplied unlimitedly throughout the experiment.

The experimental animals were sacrificed rapidly from 24 hours after ethanol administration in consideration of daily fluctuations, and were subjected to open abdominal midline under ether anesthesia and blood loss from the abdominal aorta. Liver tissue was extracted after perfusion with saline portal vein at 4 ° C saline solution, and foreign substances on the surface were removed, and rapidly frozen in -80 ° C cryogenic freezer and stored for alcohol metabolic enzyme activity. The collected blood was allowed to stand at room temperature for 30 minutes, and then centrifuged at 3,000 rpm for 15 minutes to obtain serum, and used for measuring liver function indicator enzyme activity.

3. Determination of blood ethanol concentration

The ethanol content of serum collected from blood collection over time was measured using the alcohol measurement kit (# 332-UV, Sigma Chemical Co., St. Louis, USA) prepared by modifying the method of Bucher and Redetzki (26). It measured using. That is, 10 μl of serum and 3 ml of NAD-ADH solution were carefully mixed and incubated at 30 ° C. for 10 minutes to measure the change in absorbance according to the concentration of NADH generated at a wavelength of 340 nm. The ethanol concentration (mg / dL) of the blood was calculated using a standard solution.

(1) Calculation of Pharmacokinetic parameters

The PK Analyst® computer program (Micromath, Inc., Salt Lake City, USA) was used to measure the pharmacokinetic behavior of orally ingested alcohol in the blood. Using the ethanol concentration in the blood measured from 0 to 12 hours, time-zone concentration curves were drawn and appropriate models selected, followed by pharmacokinetic parameters, eg elimination rate constants (λ1, λ2, λ3). And λ4), elimination half-life- t½ and area under the blood concentration-vs.-time curves ( AUC ). And the highest blood concentration (Cmax) and the time to reach the highest blood concentration (Tmax) were read directly from the obtained blood concentration curve.

(2) Determination of liver function marker enzyme activity in serum

Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were measured by Sigma kit (# 505-P and 505, Sigma Chemical Co., Ltd.) prepared according to the method of Reitman and Frankel (27). , St. Louis, USA), and activity units are expressed in Sigma-Frankel (SF) units per mL of serum.

Sorbitol dehydrogenase (SDH) activity was measured by modifying the method of Gerlach (28). That is, 0.25 mL of serum and 25 μL of 12 mM NADH solution were added to 0.1 M Trisma buffer (pH 7.5), incubated at 25 ° C. for 10 minutes, and 0.25 mL of 4M fructose solution was added thereto, followed by wavelength 340 nm (DU 530, Beckman, USA) measured the absorbance reduction (ΔA / min) of NADH for 3-5 minutes. SDH activity was calculated using the measured absorbance reduction rate and the absorbance coefficient of NADH (6.22 × 10 -3 M? Cm-1). Activity units are expressed in mU per mL of serum.

(3) Measurement of liver tissue ethanol metabolic enzyme activity

The extracted liver tissue was sectioned at 4 ° C, and 4% of 0.25 M sucrose solution was added to a certain amount of the liver tissue and 20% (w /%) using a homogenizer (homogenizer, Polytron PT-MR 2100, Kinematica, Switzerland). v) crushed homogenate was prepared. The homogenate was centrifuged at 600 g for 10 minutes, and the supernatant from which the nucleus and the unbranched portion was removed was centrifuged at 10,000 g for 20 minutes to obtain a mitochondria fraction. The supernatant was ultracentrifuged at 105,000 g for 1 hour to obtain a cytosol fraction.

Cytosolic alcohol dehydrogenase (ADH) and mitochondrial aldehyde dehydrogenase (ALDH) activity in liver tissue were measured according to the methods of Bergmeyer (29) and Koivula and Koivusolo (30). The absorbance of NADH generated by reacting the substrate ethanol and coenzyme NAD + for 5 minutes at 37 ° C. was measured at 340 nm, and the activity unit expressed the amount of NADH produced by 1 mg of protein for 1 minute in n mole. Protein content was measured according to the method of Lowry et al. (31) and bovine albumin was used as a standard.

(4) Statistical Processing

Statistical analysis of the experimental results was carried out using the statistical package social science (SPSS) version 7.5, the mean ± standard error for each experimental group, the significance of each group was tested by ANOVA and Duncan's test with p <0.05.

4. Results and Discussion

(1) Measurement of changes in ethanol concentration in blood over time and fluctuations in pharmacokinetic parameters

The change in serum concentrations of ethanol taken orally over time is shown in Figure 1.

The control group showed the distribution in the body for 4 hours while maintaining the highest blood concentration after 1 hour of ethanol intake. The highest blood levels of the two comparators were also slightly different, but they were distributed in the body until 4 hours of ethanol intake, similar to the control group. On the other hand, the city administration group reached the highest concentration at 30 minutes of ethanol intake, and then showed a distribution profile up to 2 hours, and then the blood ethanol concentration began to decrease relatively rapidly from 4 hours while maintaining a lower concentration than other experimental groups. At 6 hours and 8 hours, there was a significant decrease of about 26% and 81%, respectively, compared to the control group.

Based on the data measured in Figure 1, the pharmacokinetic indicators of orally ingested ethanol in blood are shown in Tables 1 and 2.

Elimination rate constant (λ1, λ2, and λ3) between all groups except λ4 value for P2 groups showing a significant reduction compared to the control group were not otherwise change elimination half-life (t½) and maximum blood concentration (Cmax) also There was no change between all experimental and control groups. However, the time to reach the highest blood concentration (Tmax) and the area under the blood concentration-time curve detected from the first 0 hours to the last time ( AUC to last time) decreased by 33% and 21% in the initial administration group compared to the control, respectively. It was shown a tendency to (Table 1). The trapezoidal AUC to last time also showed a statistically significant (p <0.05) decrease of 21% in the primary administration group compared to the control group (Table 2).

These parameters, often used in the study of pharmacokinetic behavior, are useful for analyzing the absorption, distribution, and metabolism of drugs in the body, and for determining the in vivo kinetics of drugs entering the body through different absorption pathways. This is an indicator applied. In this experiment, Tmax, AUC to last time, and trapezoidal AUC to last time (p <0.05) resulted in a decrease in the initial administration group compared to the control group, and if the blood alcohol concentration could be lowered, the hangover caused by drinking could be eliminated. Considering the general fact that it can be considered, it is thought that the city office can alleviate or eliminate the hangover symptoms caused by ethanol intake by shortening the distribution and excretion time of ethanol in the body.

(2) Serum ALT, AST and SDH Activity Variation

It is considered that the value of the product will be improved when the health drink for the hangover is first secured, and thus, the indicator of liver damage in rats acutely administered ethanol after oral hangover removal Serum ALT, AST (34-36) and SDH (37) activity changes were measured to evaluate the effect on liver function in drinking in parallel with drinking (Table 3 and Figure 2).

Serum ALT, AST and SDH activities were not significantly different between normal and all experimental groups.

These results suggest that a single dose of 3g / kg ethanol did not show any change in the biochemical markers of liver damage in 24 hours, and that drinking of hangover did not affect normal liver function.

Health drinks made with natural products are generally known to be safe, but all drugs have a dose-response relationship and may be hazardous when overdose. In this experiment, the amount of natural tea for removing the hangover of 2mL orally administered to the experimental animals is equivalent to about 10 times the weight when ingested in humans. Therefore, safety is recognized in view of the result of no observed activity change of enzymes in liver function when ingested in parallel with ethanol, and in the present invention, since an item approved by the Food and Drug Administration of Korea Food and Drug Administration was originally used when selecting raw materials, It is natural that the results came out. Nuts fruit is also known from several studies to treat and prevent liver damage caused by chemicals. In general, ingestion of the hangover is usually limited to one time before drinking. Furthermore, the long-term use of the natural hangover tea may be effective in protecting liver damage from chronic alcohol consumption. .

(3) Changes in liver tissue ethanol metabolic enzyme activity

In general, the blood concentration of ethanol ingested orally is known to be reduced by the inhibition of absorption through the gastrointestinal tract or by the promotion of ethanol metabolic rate. ADH and ALDH activities were measured in liver tissues related to ethanol metabolizing enzymes to determine the exact mechanisms compared to the control group (Figure 3).

ADH and ALDH activity in liver tissues were not significantly different between normal group and ethanol-injected group regardless of hangover type. These results suggest that the intake of ethanol 30 minutes before the ethanol administration and 3 g / kg ethanol once in rats did not affect the enzyme activity involved in the ethanol metabolism. Kim and Park observed that hongkyungcheon extract did not affect the blood concentration of ethanol administered intraperitoneally, but decreased the blood concentration of ethanol administered orally, and hepatic tissue ADH and ALDH activity did not change. It is reported that this will inhibit the absorption of ethanol through the gastrointestinal tract. In the present study, the mechanism of decrease in blood ethanol concentration and significantly lower AUC of the primary administration group was not related to the ethanol metabolism, but the reduction mechanism was not determined because the ethanol intraperitoneal administration experiment was not performed. It is difficult and is considered to be a subject for further study.

As seen above, in the natural tea for the hangover according to the present invention, which is made from Gastrodia elata , etc., is used for orally hangover 30 minutes before ethanol (3 g / kg bw, 25% in distilled) administration. Blood ethanol concentrations and pharmacokinetic indicators of ethanol taken from the aorta with time of in vivo (0.25, 0.5, 1, 2, 4, 6, 8, 10 and 12 hours) in vivo We observed the efficacy of the hangover removal effect by observing the change of the product. The safety and mechanism of the change of ethanol concentration in blood were confirmed by the measurement of liver function indicator enzyme and ethanol metabolic enzyme activity variation.

1. The intake of natural tea for eliminating hangover showed lower ethanol concentration in blood compared to the control and other experimental groups, and markedly decreased from 4 hours after ethanol administration. The blood concentration-vs.-time curves ( AUC ) showed a significant (p <0.05) decrease of about 21% compared to the control.

2. Serum ALT, AST and SDH activity fluctuations were measured in rats treated with ethanol acutely after ingestion of hangover. These results suggest that the natural hangover tea as a health drink is safe without adversely affecting the living body.

3. ADH and ALDH activities related to ethanol metabolism in liver tissue did not show any change in all experimental groups compared to the control group. These results suggest that one dose of hangover and one dose of 3 g / kg ethanol did not affect the enzyme activity involved in ethanol metabolism in rats.

In summary, all the experimental results were taken by drinking natural tea for the hangover, which contains Gastrodia elata and Hovenia dulcis Thunb. The hangover symptoms can be alleviated or eliminated, and the safety of the hangover drink made by using synthetic medicine as a raw material is also ensured, which is considered to be excellent product.

Claims (2)

Chopped and mixed with 7.5% of Cheonma, 35.0% of Raven (earth), 10.0% of Jujube, 10.0% of Duck, 10.0% of Brown Root, 9.0% of Cornus, 5.0% of Schisandra chinensis, 5.0% of Schisandra chinensis, 4.0% of Mint, and Elm 2.0%; Adding 10 times purified water to the mixture above the total weight of the mixture and performing cold extraction at 85-90 ° C. for 6 hours; Filtering the extract above to cool; Adding the following additional substances to the extract, the extract 80%, liquid fructose 12.0617%, fragrance 0.1200%, stevion 0.0050%, citric acid 0.0044%, purified water 7.8089%; characterized in that it comprises a; doing A method of producing a natural tea for hangover, which contains extracts of cheon-ma and earth tree fruit. It is produced by the method of claim 1 Natural tea for hangover that contains extracts of celestial horses and earth tree fruit.