KR20050075331A - The use of fermented wheat-germ in the feeding and veterinary practice - Google Patents

Abstract

This invention relates to new uses of fermented wheat-germ extract namely for purposes of animal feeding and veterinary therapy. The subject matters of this invention are also the fodders, nutriments, premixes and veterinary preparations containing fermented wheat-germ extract.

Description

USE OF FERMENTED WHEAT-GERM IN THE FEEDING AND VETERINARY PRACTICE}

The present invention relates to new uses of fermented wheat-germ extracts, in particular new uses for feeding and veterinary purposes. Subjects of the present invention also include feeds, nutrients and premixes containing fermented wheat pear extracts.

Fermented wheat pear extract (hereinafter referred to as VET-HBM) and its production are described in WO 98/08694, which describes the immunostimulatory and metastatic inhibitory effects of fermented wheat pear extract. (The foregoing documents are incorporated herein by reference.) This aforementioned material is produced by fermenting wheat pears with Saccharomyces cerevisiae and drying the filtered fermentation liquid. The material obtained is characterized by a 2,6-dimethoxy-p-benzoquinone component which is about 0.4 mg / g dry material.

Surprisingly, in our investigation, we found that VET-HBM can be applied well to livestock and animal feeding. VET-HBM increases body weight faster and increases the animal's resistance to diseases, particularly infectious diseases. This serves in particular to increase the yield of meat and to improve the meat quality of farm animals, especially poultry and pigs, which are raised under large production environments, while at the same time providing better utilization of feed (better feed conversion ratio).

Economically large-scale production of pigs and poultry is an important factor in determining the profitability of livestock. Reducing feed volume is of great importance because feed prices are high. In the last 10 years, many good results have been achieved in the quantitative and qualitative indicators of the meat and eggs produced, with the help of the development of professionally synthesized feeds, and with the application of appropriately selected supplements. However, on the one hand it has been proved that more efficient feed ingredients, in particular feed additives, cannot be applied without worry (toxicity, resistance). That is, their efficiency decreases after a certain period of time, and they can be opposed in terms of environmental protection and possibly public health. After all, the object of the present invention is to find a body-identical and natural substance that can be effectively applied to the feeding and breeding of farm animals. This object meets the needs of the European Union to suppress antibiotics and body-abnormal substances in the field of yield enhancers.

In the course of our investigation, we attempted to produce the above-mentioned nature-based VET-HBM under the large-scale production environment of broiler chickens, geese and turkeys as well as pigs during preparatory and fattening periods after weaning. . VET-HBM was properly introduced into the tissues of the animals by mixing into a conventional feed. In the course of our experiments, VET-HBM improves body weight gain and specific feed utilization, increases resistance, and simultaneously reduces environmental contamination, thus supplementing early-, rearing- and final nutrition with VET-HBM. Has been shown to have a beneficial effect on the development of broiler chickens, geese, turkeys and pigs. In addition, the inventors have found that the egg viability and, consequently, the hatching frequency increase in hen cattle. In addition, the inventors have found that aorta cleft, which is often accompanied by rapid growth when fattening turkeys, can be substantially eliminated, which is particularly beneficial.

In addition, we studied the effects of VET-HBM on infections that typically occur in poultry farms, and surprisingly, mycoplasma (as well as coccidiosis, which VET-HBM is caused by Eimeria tenella), Protect animals against infections caused by Mycoplasma microorganisms, in particular, M. gallisepticum and M. synoviae, and other diseases (e.g. (Gumboro disease) has been shown to increase resistance.

Livestock poultry contamination by Mycoplasma gallicepticon and Mycoplasma sinobia still causes significant economic losses in poultry farming. As a result of this infection, gain of body weight and egg production decrease, and death, hatching loss, confiscation in slaughterhouses, etc. increase. Epithelial damage caused in the respiratory tract persists in a second bacterial infection, thereby further increasing losses.

In order to reduce economic losses, the use of other antibiotics (eg, tilosin, thiamuline, norfloxacine, enrofloxacine, etc.) is proposed. However, in recent years, authorities in other countries have desired to reduce the use of antibiotics (eg, the use of some antibiotics, including tylozin, has been banned as yield sensitizers), and they force the use of antibiotics used for human purposes. Claimed to do. For this reason, we investigated the effects of VET-HBM and provided very good results from previous feeding attempts against a widespread mycoplasma infection.

Surprisingly, the inventors have found that administration of VET-HBM can prevent the effect of mycoplasma infection, similar to the administration of tiamutine, best known as an anti-mycoplasmic antibiotic. Since mycoplasma infections are quite common everywhere in the world, this fact is of great economic importance. Economic losses caused by mycoplasma infection can be reduced by means of VET-HBM use. It may be particularly desirable when the use of antibiotics is attempted to be compelled in veterinary use and yield enhancement.

In addition, we investigated pigs in connection with M. hyopneumoniae present in the common swine population and causing very large economic losses, and surprisingly VET-HBM has been investigated in mycoplasma hyopneumoniae. It has been found that pigs can be protected against pneumoniae caused by the disease.

In addition, the present inventors investigated the effect of VET-HBM on coccidiosis, another parasitic disease caused by very widespread parasitic diseases in poultry. Because mass breeding forms have increased the chances of coccidia infection to the highest extent, the number of coccidia cases has increased anywhere in the world in recent decades. Seven strains of Emeria play a role in the formation of coccidiosis, and among these, pathogenic strains and weak pathogenic strains are likely. Among the strains that cause hemorrhagic enteritis and death, Emeria Tenella, which causes cecal coccidiasis, has considerable importance. Therefore, we prepared chickens infected with pure Demerian tenella strains.

Surprisingly, the inventors found that excretion of the conjugated sac of artificially infected chickens obtained VET-HBM supplements was reduced compared to the control group, which destroyed the intermediate form and caused significant damage to the cecum. It means that the parasite could not develop. As a result, VET-HBM can protect chickens against serious disease caused by E. tenella.

In addition to the foregoing, we studied the effect of VET-HBM on changes in antibody levels in chickens vaccinated against disease with Gambo. We found that VET-HBM administered to feed significantly increased antibody production in chickens, with an increase in antibody levels, which enhances the effectiveness of the CEVAC vaccine, which provides higher protection to animals during chicken hatching. Came out.

In addition, the inventors have observed that administration of VET-HBM significantly reduces the economic loss caused by stress effects (eg, heat and transport stress).

Based on the above findings, the present invention relates to the use of fermented wheat pear extract as feed supplement for the production of animal feed, nutrition or premixes. In the term used in the present invention, the term "animal" refers first to farm animals such as cattle, horses, pigs, poultry, rabbits, farmed fish; Zoo animals as well as pets and other domestic animals such as dogs and cats. The feed supplement according to the invention can be used as a yield enhancer for farm animals, preferably poultry such as broiler chickens, hens, roast goose, feather goose, liver goose, roast duck, turkey, as well as pigs and piglets.

According to another aspect, the present invention relates to a feed, nutrient and premix containing fermented wheat pear extract in addition to known feed, nutrient and premix ingredients. The feed and nutrients of the present invention contain wheat pear extract in an amount of about 0.001-10% by weight, preferably 0.01-5.0% by weight, most preferably 0.3-1.0% by weight. The feed and nutrient premix according to the present invention may contain wheat pear extract in an amount of about 0.001-50% by weight. The preparations according to the invention are prepared by mixing the fermented wheat pear extracts in a manner known per se with the aforementioned amounts of solid forgeable excipients, in the case of premixes, conventional vitamins and micro-elements and feeds, respectively. Prepared in such a way.

VET-HBM supplements according to the present invention are applied to the initial, rear and end feeds or nutrition, respectively, in part, or in all rearing processes, in combination. VET-HBM may also be applied to drinking water in animals.

According to another aspect, the present invention relates to a method for enhancing yield of farm animals. According to this method, fermented wheat pear extract as a yield enhancer is given to the animal's feed, which is fed the feed. The yield enhancer described above is applied in an amount of 0.1-6 g / kg feed, preferably 0.3-3 g / kg feed.

According to another aspect, the present invention provides an animal for preventing and / or reducing poultry coccidiosis infection, mycoplasma infection and infectious inflammation, and for enhancing antibody titers of vaccinated poultry. To the use of fermented wheat pear extracts. The fermented wheat pear extract is used to prevent mycoplasma galilicepticum or mycoplasma sinobia infection, to prevent and / or reduce pox's coccidia infection, as well as by mycoplasma hyopneumoniae in pigs. It can be advantageously used to prevent the resulting pneumonia. The present invention relates to the use of fermented wheat pear extract in the preparation of a formulation for the aforementioned purposes.

Veterinary preparations according to the invention containing fermented wheat pear extracts can be prepared in a conventional manner and are formulated to enhance the animal's resistance by mixing the active ingredient with one or more veterinary acceptable auxiliary materials, myco And agents that prevent and / or treat plasma infection and infectious inflammation, agents that prevent and / or treat poxidiosis infection in poultry, and agents that increase antibody titer of vaccinated poultry.

Formulations using ancillary materials typically applied to veterinary use can be formulated into tablets, pills, capsules, gels or pastes. These auxiliary materials are gelatin, natural sugars such as row sugar, lactose, maltose and dextrose, lecithin, pectin, cyclodextrin, dextran, polyvinylpyrrolidone, polyvinyl acetate, acacia rubber Xanthan gum, tragacanth, agar-agar, alginic acid, carboxymethyl cellulose, carboxymethyl cellulose sodium, hydroxypropyl cellulose, hydroxypropyl methyl cellulose or similar cellulose derivatives, emulsifiers, oils , Polyglycerol esters and glycerol esters derived from fats, in particular saturated fatty acids.

The amount of components in the formulation can vary, depending on various factors such as the individual needs of the animal to be treated. The dosage may depend in particular on the size of the animal to be treated and the type of disease to be prevented or treated. The daily dose may be administered in a single dose or divided into more partial doses per day.

The invention is further illustrated in the following examples, which are not intended to limit the invention.

The VET-HBM used in the examples below was prepared according to the following technique which corresponds substantially to Example 2 of WO 99/08694.

300 kg of crushed wheat pears (according to the Hungarian standard) and 100 kg of yeast (Saccharomyces cerevisiae) were placed in a 5 m ³ fermentor and drinking water was added until the volume reached 4000 L. The period was 18 hours, during which a continuous air supply (0.5 L air / L fermented liquid / min) and slow stirring (30 rev./min) were carried out 1 L / m ³ to prevent foaming Sunflower oil of was added to the mixture, after fermentation, the air supply and stirring were stopped, and the fermented liquid was first separated by a screw decanter, then by a separator, and finally by a sharpening separator.

Preparation of fraction 1

The fermented liquid was filtered sharply and the sharpness was confirmed microscopically. The filtered fermented liquid was substantially free of cells, meaning that up to one yeast cell was found up to 10 sight. The resulting fermented liquid, containing about 1.5% by weight of dry matter, was evaporated in a vacuum condenser at a temperature of 40-50 ° C., and the vacuum was stopped and then heated at atmospheric pressure for about 15 minutes. The dry matter content of the solution was then measured and first dissolved in hot water and then cooled maltodextrin was added to bring the dry matter content of the solution to about 30% by weight.

The solution was then spray dried in a shear nozzle rotating the spray dryer, where the temperature of the exiting air was about 90 ° C. The resulting final product contained 60% by weight fermented plant material and 40% by weight maltodextrin as powder. The dimethoxy-p-benzoquinone content measured by HPLC was 0.15 mg / g dry matter ± 20%.

Preparation of fraction 2

Biomass with 25-27% by weight of dry matter separated in a screw decanter is dried in a ratio of 1: 1 with respect to the finely ground crushed corn carriers in a fluidization dryer plant and granulating its granule size By 0.2-0.8 mm.

Preparation of the final product

Fractions 1 and 2 were mixed in a homogenizer of the Lodige system and carefully homogenized. The 2,6-dimethoxy-p-benzoquinone in the formulation obtained in this manner was 0.11 mg / g dry matter ± 20%.

Example 1

32,600 day old Broiler chicks (Shaver Starbo) were fed and three groups were formed from them. The control group "K" consisted of 16300 animals and the two experimental groups ("I" and "II") consisted of 8150-8150 baby chicks. The internal content of the feed corresponds to the requirements specified at the current breeding stage. VET-HBM standardized formulations were mixed in the initial, rearing and end stage nutrients of animals of the experimental groups "I" and "II" in an amount of 3 g / kg of feed.

Animals of the control "K" group and the experimental "II" group were treated with enrofloxacine [Avian Pathol. 19 , 511-522 (1990). Animals in experimental group "I" were not subjected to enrofloxacin and any other medical treatments common to chicken breeding. To prevent Gumbo-disease, the CEVAC vaccine (Phylaxia of Budapest, Hungary) was equally mixed in the drinking water of animals in all three groups.

The feeding system was automatically managed and connected to two tanks capable of holding 10-10 tons of feed. Changes in feed occurred gradually. Cover straw (litter) was about 120 m ³ dry pine pieces covered with a 6 cm depth which corresponds to about 100 ton at every change of the cover straw manure. Ventilation was solved by 44 ventilators with speed regulators, and the ventilator capacity was 10000 m ³ / hour, respectively.

result

1. Measurement of the number and percentage of deaths

In broiler chicken feeding experiments, hatching weakening as well as unusually hot summer temperatures (heat shock) resulted in mortality greater than normal mortality (5.3%). The mortality rate in the course of the experiment was 5.57% (913) in the control group, 4.9% (400) in the experimental group “I” and 4.04% (330) in the experimental group “II”.

Thus, based on the mortality rate it can be observed that VET-HBM has been of considerable help in the prevention of heat shock.

2. Measurement of Percentage of Weekly Weight Gain

In the course of the feeding experiment, weight gain to reduce breeding weeks was greater in all cases in experimental animals that obtained VET-HBM adjuvant than in animals in the control group. The results of weekly mass measurements and weekly weight gain percentages of the control group are shown in Table 1 below.

Weekly weight measurement of chicken Breeding week Group "K" (g) Group "I" (g) Weight gain (%) Group "II" (g) Weight gain (%) One 150 153 102.0 157 104.66 2 372 375 100.8 379 101.88 3 736 740 100.5 749 101.76 4 1232 1276 103.57 1288 104.54 5 1580 1589 100.56 1597 101.07 6 1953 2001 102.45 2055 105.22

3. Determination of the formation of fat indicators

The weight gain of the animals obtained with the VET-HBM adjuvant from Table 2 exceeded the value of the control group at the end of the experiment, exceeding 2.45% in group "I" and 5.22% in group "II".

Conversely, the specific utilization of the feed (feed conversion ratio) is lower in the experimental group than in the control group of group "K", which did not obtain auxiliary material, almost 12% (1.85 kg / kg) and 12.4% (1.84 kg / kg) lower in the experimental group "II".

Effect of VET-HBM on Large Breeding of Broiler Chickens Measure Control group "K" Experiment group "I" Experiment group "II" Initial animal count (100%) n = 16300 n-8150 n = 8150 Initial average weight (kg) 0.055 total: 896.5 0.053 total: 431.9 0.052 total: 423.8 Final animal count as a percentage of initial number n-1537094.00% n = 775095.09% n-782095.95% Final total weight (kg) 30017.6 15507.7 16070.1 Final Average Weight in Kg 1.953100% 2.001102.45% 2.055105.22% Total feed consumption 61154.3 27890.1 28789.2 Feed conversion ratio (kg / kg) 2.10 100% 1.8588.1% (-11.9) 1.8487.6% (-12.4)

In addition, the deadline for transporting broiler chickens was shortened by one week, and the slaughter experiments confirmed that the yield of lean meat, in particular breast meat and leg meat, was increased.

It should be noted that the medical treatments applied to conventional breeding techniques to date have been excluded from animals obtained only experimental group "I" and only VET-HBM. In spite of this fact, these chickens proved to be resistant like the members of the experimental group "II" which obtained conventional breeding techniques supplemented with VET-HBM.

The concentration of feces changed, the number of diarrhea decreased, and the concentration of litter improved due to the excretion of harder feces. This plays a larger role in terms of environmental protection, because it requires less change in the bulk of the straw than to save material and manpower.

We obtained similar results when VET-HBM was applied in an amount of 0.3 g / kg feed.

Example 2

In pig cages, under the environment of large scale production, three groups of 35 day old milked bacon piglets were tested, all groups 50-50. During nearly 60 days of feeding preliminary experiments, 3 g VET-HBM was mixed with the animal's 1 kg feed. Control animals were fed a feed that has been common in the present manufacture. However, two groups of experimental animals consumed feed containing VET-HBM supplementary materials from 35 to 92 days of age.

When weaned, the lids of the animals are divided into two of them, the first formed control group is "A" and the second formed group of experimental animals is "B" and "C", thereby generating genetic elements. Was removed. In this experiment, all three groups of the animals were mixed sex. In the rearing process, breeding, feeding and drinking techniques used are common in the present production. The piglets were fed Starter piglet nutriment from 35 to 95 days of age. Feed administration was carried out by the feeding facility of the Big Dutchman MC44-V03 system. Daily feed consumption was recorded by an LCD SCAN type feeding computer. In the course of the experiment, the following were recorded in both the control group and the experimental group:

-Number of initial and final animals,

The weight of the animal at the beginning of the experiment,

-Feed consumption by group,

-Hygiene, changes occurring in the clinical condition of the animal and the reasons for intermittent diseases and deaths,

Last average individual weight and total weight (individual biomass)

The results are shown in Table 3.

Effect of VET-HBM on the Breeding of Piglets Measure Control group "A" Experiment group "B" Experiment group "C" Initial animal count (100%) n = 50 n = 50 n = 50 Initial average weight (kg) 12.26 total: 613 12.04 total: 602 12.05 total: 602.5 Final animal count as a percentage of initial number n = 4794% n = 4998% n = 50 100% Final total weight (kg) 1385.5 1523.9 1613.0 Final Average Weight in Kg 29.48100% 31.10105.4% 32.26109.43% Total feed consumption (kg) 1637.7 1843.8 1930.0 Feed conversion ratio (kg / kg) 2.12100% 2.0094.33% (-5.67) 1.9190.09% (-9.91)

From Table 3, supplementation of VET-HBM to piglet feed reduced death levels. VET-HBM fed with feed in piglets 35 to 92 days of age had an effect on body weight gain in animals. That is, the weight of piglets of group "B" exceeded 5.4% (31.10 kg) of the control animal weight, whereas the weight of piglets of group "C" exceeded 9.43% (32.26 kg) of the weight of the control animal. .

Feed conversion ratios (specific utilization of feed) were lower than in control animals ("A") who did not receive supplements, ie 5.67% in animals of group "B" and 9.91% in animals of group "C".

Fecal concentrations changed and diarrhea did not occur in piglets in all experimental groups. Due to the excretion of hard excreta, the cover straw concentration was improved in all cases than in the control animals.

Example 3

Based on the smooth results, the administration of VET-HBM continued until the end of fattening. During the fattening process from 95 days of birth to the day of slaughter (172 days), pigs consumed fattening nutrients. In this case too, the feed contained 3 g VET-HBM. The results are shown in Table 4.

From Table 4, it can be seen that there was no death until the end of the fattening. Terminal pig weights were higher than those of the control group (108 kg), ie 1.8% (110 kg) higher in group "B" and 5.5% (114 kg) higher in group "C".

Feed conversion ratios (specific utilization of feed) were 10.9% and 15.2% lower in the animals of the experimental group than in the control animals, respectively. The concentration of excreta changed and diarrhea did not occur in the experimental animals. However, in the case of control animals, diarrhea swine developed.

 Effect of VET-HBM on the Breeding of Fat Pigs Measure Control group "A" Experiment group "B" Experiment group "C" Initial animal count (100%) n = 47 n = 49 n = 50 Initial average weight (kg) Total: 1385.56 31.10 total: 1523.9 32:16 Final animal count as a percentage of initial number n = 47100% n = 49100% n = 50 100% Final total weight (kg) 5076 5390 5700 Final Average Weight in Kg 108100% 110101.8% (+1.8) 114105.5% (+5.5) Total feed consumption (kg) 12213.9 11404.7 11484.4 Feed conversion ratio (kg / kg) 3.31100% 2.9589.1% (-10.9) 2.8184.8% (-15.2)

Example 4

Feeding experiments were conducted under the environment of large-scale production of roast goose and studied the effects of VET-HBM. Freshly hatched hybrid goslings of the 250-250 first class were tested, one of these groups as the experimental group and the other as the control group. The internal content parameters of the feed corresponded to the requirements defined for the current breeding stage. In the experimental group VET-HBM adjuvant was mixed in the animal's initial, rearing and terminal nutrients in an amount of 0.3 g / kg feed.

Animal quarters corresponded to current regulations for geese breeding (number of birds: 8 birds / m ² ). From day 3 to day 14 after approval, the room temperature of 32 ° C was gradually lowered to 20-22 ° C. Natural light was supplemented with artificial light in the course of the preliminary breeding period. In the course of the preliminary breeding period (4 weeks), the intake of the water of the animal was carried out through a tip drinking apparatus, followed by a pipe drinking apparatus at will.

In the course of the experiment, the following variables were recorded in both groups:

-Number of early and late animals,

-Clinical condition,

-Loss of death, indicating the reason for death,

Individual weight at 28 and 55 days of age, and

Feeding ratios at 28 and 55 days of age.

The results are shown in FIG.

From the results obtained, it can be seen that nutrition supplemented with wheat pear extracts fermented in goose breeding can be supplied very efficiently.

Effects of VET-HBM on Roasting Goose Breeding Measure Control group Experimental group Initial animal count (100%) n = 250 n = 250 Initial average weight (kg) 0.087 total: 21.75 0.087 total: 21.75 Number of final animals in control group percent n = 23895.2% n = 24196.4% (+ 1.2%) Final total weight (kg) 1193.33 1265.00 Average body weight (kg) at 28 days as a percentage of the control 2.030 100% 2.167106.74% (+6.74) Average body weight (kg) at 55 days as a percentage of control 5.014100 5.249 104.68 (+4.68) Feed conversion ratio (kg / kg) of 28 days as a percentage of the control 2.52 100% 1.5994.09% (-5.91) Feed conversion ratio (kg / kg) of 55 days as a percentage of the control 2.78 100% 2.5290.65% (-9.35%)

The clinical status of the experimental animals did not show any variation compared to the control animals. In the last 8 weeks of the preliminary breeding period, mortality in the experimental group decreased by 3.6% compared to the 4.8% value in the control group. The experimental group showed significant weight gain compared to the control group. By day 28, body weight gain in the experimental group was 6.7% improved over that of the control group, whereas at 55 days of age, the weight of the roast goose in the experimental group exceeded the body weight of the control group by 4.7%.

The utilization of feed in the experimental group improved significantly. The feed conversion ratio (specific feed utilization) was improved in the experimental group than in the control group, being 5.9% at the first 28 days and 9.35% lower until 55 days.

Example 5

Feeding experiments were conducted in broiler turkeys under the environment of large scale production, and the effects of feeding of feed supplemented with VET-HBM were investigated.

Experiments were carried out with turkeys of 1 day old, divided into four groups. The control group included 9300 females (A) and 8700 males (C) turkey pups, and the experimental group included 9600 females (B) and 9100 males (D) of meat type (BIG-6). Turkey chicks were included. 0.3 g of VET-HBM was added per kg of feed to the animals of groups B and D.

The experiment was carried out on a large turkey farm in which one day old meat hybrid turkey pup (origin: Nadudvar, Hungary) designated BIG-6 (Gigant) was introduced into the aforementioned group. Installation density was the same in all 4 groups (4 mice / m ² ). In buildings using thick straw breeding techniques, room temperature, ventilation and humidity are guaranteed to meet current age in accordance with technical regulations. Broiler turkey feed was the initial, rearing and end turkey nutrients common for breeding and fattening turkeys, respectively (they were combined according to the provisions of the Hungarian Feed Code (1990)); These feeds were supplemented with 0.3 BET-HBM per kg in experimental groups B and D.

The animals were fed initial nutrients up to 56 days of age, breeding nutrients from 57 to 112 days of age, and late nutrients from 113 days of age to the end of fattening. For the prevention and treatment of bacterial infections, Lincospectin, which is common for turkey fattening, was used in all four groups.

In the course of the experiment the following was recorded in both the control group and the experimental group:

-Number of initial and final animals,

-Loss of death, indicating the reason for death,

-Weight at the beginning and end of the experiment,

-Changes in health, clinical condition,

-Technical deficiencies in the breeding process, and

-Feed utilization data.

The obtained result is shown in FIG.

Effect of VET-HBM on turkey fattening Measure female cock Control group (A) Experiment group (B) Control group (C) Experiment group (D) Early animal count n = 9300 n = 9600 n-8700 n = 9100 Initial total weight (kg) 586 595 539 564 Initial average weight (kg) 0.063 0.062 0.062 0.062 Final animal count as a percentage of control n = 880494.66% n = 930796.94% (+ 2.28%) n = 819294.16% n = 871495.75% (+ 1.58%) Final total weight (kg) 77823 91581 138772 161906 Final average body weight in kg of control 8.76 100% 9.84112.32% 16.94100% 18.58109.68% Total feed consumption (kg) 12213.9 11404.7 11484.4 Feed conversion ratio as percentage of control (kg feed / kg weight gained) 3.27100% 3.0793.88% (-6.12) 3.24 100% 3.0594.13% (-5.87)

The clinical status of the experimental animals did not show any deviations compared to the control animals. Additional animals in the control group died 2.28% and 1.58%, respectively.

At the end of the experiment, body weight gain was greater in the two experimental groups, 12.32% in Group B and 9.68% in Group C.

The feed conversion ratio was also improved in the two experimental groups, which was 6.12% less in Group B and 5.87% less in Group C, respectively, compared to the control group.

It should be noted that the fact that aorta cleft and death due to rapid growth in the course of turkey fattening do not exist as a result of the administration of VET-HBM is of great advantage.

Example 6

Investigation of the effects on mycoplasma gallicepticon

This study was conducted with Arbor Acress chickens of meat types not infected with Mycoplasma sinobia. Freedom from Mike Plasma sinobia in animals has been demonstrated by histological serological screening studies in agglutination tests with the help of Mycoplasma gallicepticum and Mycoplasma sinobia antigens (Intervet International BVm; Boxmeer, The Netherlands). ). In addition, animals were performed in ELISA-tests based on monoclonal antibodies and using MYGA test-kits (Diagnosztikum Kft; Budapest, Hungary) and MYSA kits (Svanova, Uppsala, Sweden) (Czifra, Gy. et al .: Avian Dis. 37 , 680-688 (1993)]. The serological investigation gave a negative result. In addition, from the same incubation obtained with experimental animals, isolation of mycoplasma was isolated from the nasal cavity, trachea and air pockets of 20 day old chicks. Scand. 14 , 436-449 (1973)] and Frey's media [Frey, MC et al .: Am. J. Vet. Res. 29 , 2164-2171 (1968). This culture also closed with negative results.

120 animals were divided into four equal groups of equal numbers (30-30 animals), so that the average weight of the four groups did not deviate from each other in the Student's test.

For infection of experimental animals, Mycoplasma galilicepticon N ⁰ 1226, previously amplified for 24 hours in medium B, was used. The pear content was 9.5 × 10 ⁸ pfu / ml (pfu = flag forming unit).

The experimental groups were treated and infected as follows:

Group 1 was placed in a tightly sealed 200 liter box, sprayed with 10 ml sterile medium B therein, and the animals were kept in the box for 20 minutes. This group was then placed in an isolated room and no treatment was done to the animals. This group was considered as a negative control group.

Group 2 was placed in the same box sprayed with 10 ml Mycoplasma Galicepticum broth medium and the animals were kept in this box for 20 minutes. The group was then placed in an isolated room and no treatment was done. This group was considered as a monitoring control of infection.

Group 3 was infected in the same manner as Group 2, then placed in a third room and fed chick breeding nutrients containing VET-HBM at a concentration of 3 g / kg during the experiment.

Group 4 was infected in the same manner as Group 2, then placed in a fourth room and fed animals with nutrition containing 200 mg / kg of tiamutine (Biochemie GmbH, Kundl, Austria) It was.

To determine the effectiveness of the treatment, clinical signs, weight changes, feed conversion ratios were examined, in addition to pathological, histological and serological investigations as well as re-separation of mycoplasma. In the course of these evaluations, the following results were obtained.

result

1. Clinical investigation

Clinical signs and possible deaths were examined daily. There were no clinical signs in the treated animals (groups 3 and 4), whereas respiratory signs appeared after 6 days in the infected, untreated group, and 1-1 deaths occurred after 7 days and 9 days.

2. Gain weight

Body weight gain was statistically significantly lower in the infected, untreated group than in the untreated group of the control as well as the two treated groups. At the same time, in the two treated groups, body weight gain was to the same extent as the control group.

3. Feed Conversion Ratio

The feed conversion ratio increased to 0.45 kg / kg in the infected, untreated group, while maintaining the same level as the control group in both treated groups.

4. Pathological investigation

At the end of the experiment, all animals were examined for air pockets and peritoneal inflammation characteristic of mycoplasma gallicepticum infection by pathological dissection.

In group 1 all animals were negative, whereas in group 2 all animals showed airpocket and peritoneal inflammation with different severity. Pathological changes developed significantly more difficult in treated Groups 3 and 4, and their severity was significantly moderate than in Group 2 animals. The results of the groups treated with VET-HBM and thiamutine respectively did not differ from each other.

5. Histological investigation

In group 2, the number of lymphohystiocytic bronchitis and lobular interstitial pneumoniae as a result of infection increased significantly compared to uninfected group 1. At the same time, the variables in groups 3 and 4 treated with VET-HBM and thiamutine, respectively, remained at the same level as group 1, except that the number of lobulary interstitial pneumonia was significantly higher in group 3 than in the control group. . Groups 3 and 4 were not statistically different from each other as long as the investigated changes were considered.

6. Serological investigation

Plasma of all chickens was examined on slides with mycoplasma gallicepticum deadlock test. The severity of the response was recorded and the number of reactive animals and the sum total of the records was compared in a Chie square test. Group 1 remained negative until the end of the experiment. In groups 3 and 4 treated, significantly fewer animals showed serological responses (6 and 8 for 25 animals each) and the recordings were significantly lower than those in untreated group 2 (75 scores). And 11).

7. Mycoplasma Re-Separation

Reseparation of infectious mycoplasma strains was performed as follows. One hour after infection, 5-5 animals died. A 1-1 cm long tracheal piece was placed in 2 ml of liquid medium B, shaken for 3 minutes and then fold counted in the medium. Re-separation of strains used for infection at the end of the experiment was carried out using tampons from all chicken respiratory tissues (organs, lungs, air pockets) and other tissues (brain, liver, spleen, kidneys, heart) using tampons. Attempts were made to transport the sample from one organ to solid medium B. The agar slant was incubated for 10 days and then evaluated. Part of the isolates were identified by epifluorescent method using specific immune serum.

Immediately after infection, mycoplasma was not successfully isolated from the organs of group 1 animals. At the same time, 1 × 10 ² -2.7 × 10 ³ pfu / ml mycoplasma gallicepticum could be expressed from the organs of infected animals in group 2.

 At the end of the experiment, strains used for infection from group 1 could not be recultivated, whereas from group 2, especially from organs, lungs and air pockets, they could be recultivated in 64 cases. On the other hand, regrouping was significantly more successful (10 and 3 times, respectively) from Group 3 and Group 4, and from only some lungs and organs, but not from other internal tissues. No substantial difference was observed between the groups treated with VET-HBM and thiamutine, respectively.

Example 7

 E. Investigation of Effects on Tenella

48 day-old chickens were divided into 4 groups (12-12 animals in each group). The chicks were grouped in cages and the room temperature was 28 ° C. during the experiment. In the breeding process, animals consumed their usual initial nutrients and drinking water up to 14 days of age (group 1 and group 2). Nutrients from group 3 and group 4 were supplemented with 0.3 g VET-HBM per kg of feed.

Animals of group 2 and group 4 were infected with a suspension containing 2 × 10 ³ Emeria tenella sporulated conjugated sac.

Conjunctival sac excretion was observed in the feces starting 7 days after infection. Daily amounts of feces from animals belonging to the same group were weighed and homogenized individually. The same amount was measured from the excreta of each animal and it was homogenized with a 2.5% KrCr ₂ O ₇ solution. Daily conjunctival sac excretion of a given group was measured three times in a McMaster chamber. The results are shown in Table 7.

Measurement of Daily Junctional Cap Excretion in a McMaster Chamber Control group Processing group Group 1 uninfected Group 2 Infection, Mean ± SD Group 3 uninfected Group 4 Infection, Mean ± SD 1 day 0 21 500 ± 0.02 1 day 0 23 800 ± 0.03 2 days 0 120 500 ± 0.31 2 days 0 27 250 ± 0.07p <0.0001 3 Day 0 84 500 ± 0.11 3 Day 0 20 300 ± 0.08p <0.0001 4 Day 0 75 800 ± 0.22 4 Day 0 15 900 ± 0.14p <0.0001 5 Days 0 5 700 ± 0.13 5 Days 0 1 800 ± 0.02p <0.0001 6 days 0 950 ± 0.03 6 days 0 200 ± 0.01p <0.001 7 days 0 350 ± 0.02 7 days 0 15 ± 0.01p <0.001

It can be seen from Table 7 that the development of the conjugate sac in infected group 4, which obtained VET-HBM, was significantly less than in the infected group 2 animals living with traditional nutrients (p <0.0001 and p <0.001). In this group, the increase in excretion of the junctional sac significantly exceeded the treatment for several days, and this high level was maintained until the end of the experiment.

Body weight measurements on Days 0, 7, and 14 showed that body weight in artificially infected animals surviving with traditional nutrients gradually decreased until the end of the experiment, whereas in the infected and uninfected chick groups consuming VET-HBM It was demonstrated that a significant (p <0.001) acquisition was recorded.

Example 8

Determination of Antibody Levels in Vaccinated Chickens

A group of 7-7 day-old chicks, Ross-308 (source: Babolna, Hungary) were tested, and the chicks were first treated with eggs as a vaccine against disease with infectious gamma (Hungarian Budapest Philaxia) (CEVAC vaccine prepared from Phylaxia). As a basic nutrient for half the chickens, the original VET-HBM was given in an amount of 0.3 g / kg feed. The animals consumed feed and drinking water freely. Control and treated chickens were bleeding gradually on the first day and weekly, respectively. Their blood was collected separately, serum was separated by centrifuge and stored at −18 ° C. until processing.

The amount of antibody was measured by ELISA test. In the course of the test, the antigen is typically absorbed into the walls of a polystyrene plate having 96 wells. The specific antibody of the serum to be investigated is bound to the antigen but the unbound antibody is removed by washing and the system is filled with the species-specific antiglobulin serum conjugated with horseradish peroxidase or another effect. Antiglobulin-conjugated molecules that failed to participate in the reaction are removed by washing. The "sandwich", the antigen-antibody-antiglobulin conjugate, is visualized in the form of a color reaction by the addition of an enzyme substrate. In this experiment, the kit tested infectious bursitis antibody (ProfFLOK) IBDELISA Kit, manufactured by Guilford Kirkegaard & Perry Laboratories, Cat. No. 54-81-01), was used for the measurement.

The measurement was performed according to the method described above. For this measurement 50-50 μl serum was added to plate wells sensitized with antigen. Positive and negative control sera were placed in the wells of the front (-1, +2, -3) and terminal (-94, +95, -96) portions of the ELISA plate. The plates with serum were incubated for 30 minutes at room temperature, then they were washed with a washing solution (300 µl) and the solution was kept in the wells for 3 minutes and then drained. This washing step was repeated after 2 minutes. At this time, 100 μl conjugates from the kit were added to the samples for each well, and then they were incubated for 30 minutes at room temperature, and finally they were washed twice as described above. Thereafter, 100 μl substrate was added to the system, which was incubated for 15 minutes at room temperature. The reaction was stopped with 100 μl stop-solution. The developed green-blue color was read at 405-410 nm with an ELISA reader. Antibody titers were calculated from the obtained absorbance data measured to decrease weekly.

From these measurements it can be seen that the serum titers of the animals bleeding on day 1 were the same as the normal values of the control group (mean 13.5). The titer of serum samples collected at 1 week was increased compared to that of the control group (mean 17.4). At 2 weeks, this growth was further increased compared to the control group (mean 21.2). At 3 weeks, a strong increase in titer was observed under the influence of VET-HBM compared to the control group (mean 30.3). At 4 weeks, the titer of the treatment group tripled compared to the control group (mean 42.1). At week 5, the titer was four times higher than the value in the control group (mean 55.6). At week 6, the titer was nearly five times higher than the value in the control group measured at week 6 (mean 69.4).

Based on statistical evaluation, it was demonstrated that the values obtained were significant (p <0.001) and that they showed a sharp increase compared to the control group.

The present invention relates to a novel use of fermented wheat pear extracts, ie for the purposes of feeding and veterinary treatment of animals. Subjects of the invention are also feeds, nutrients, premixes and veterinary preparations containing fermented wheat pear extracts.

Claims (15)

Use of fermented wheat-germ extract as feed supplement for the production of feed, nutrients or premixes. Feed or nutrition containing fermented wheat pear extract in an amount of 0.001-10% by weight in addition to the usual feed and nutritional ingredients. A feed or nutrient premix containing fermented wheat pear extract in an amount of 0.001-50% by weight in addition to the usual premix ingredients. The feed of claim 2 or 3, wherein the wheat pear extract is obtained from a fermentation broth obtained during fermentation of wheat pear in the presence of Saccharomyces cerevisiae in an aqueous medium, Nutrition or premix. A fermented wheat pear extract is provided as a yield enhancer for animal feed, and the feed obtained in the above manner is fed to the animal. 6. A method according to claim 5, wherein the yield enhancer is used in an amount of 0.1-6 g / kg feed, preferably 0.3-3 g / kg feed. The method of claim 5 or 6, wherein the farm animals are cows, horses, rabbits, piglets, fattening pigs, broiler chickens, egg-laying hens, turkeys, geese or ducks. Use of fermented wheat pear extract for enhancing yield of farm animals. Use of fermented wheat pear extract for the preparation of a formulation for preventing and / or treating mycoplasma infection in animals. Use of fermented wheat pear extract for the preparation of a formulation for preventing and / or treating pneumonia caused by infectious inflammation of animals, in particular M. hyopneumoniae. Use of fermented wheat pear extract for the preparation of a formulation for preventing and / or treating poxidia infections. Use of fermented wheat pear extract for increasing antibody titer of vaccinated poultry. Use of fermented wheat pear extract to prevent and / or reduce poultry coccidiasis infection and / or infection of Mycoplasma gallisepticum or Mycoplasma synoviae. Use of fermented wheat pear extract for the prevention of pneumonia caused by Mycoplasma hyopneumoniae. The use according to any one of claims 8 and 11 to 13, wherein the wheat pear extract is mixed and used in a conventional feed in an amount of 0.1-6 g / kg feed.