CN115380993B - Clathrate compound containing baohuoside I, composition, preparation method and application thereof - Google Patents
Clathrate compound containing baohuoside I, composition, preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to the field of livestock consumption products, and particularly discloses a baohuoside I-containing clathrate compound, a baohuoside I-containing composition, a preparation method and application thereof. The baohuoside I glucan inclusion compound is prepared from the following raw materials in parts by weight: 20 to 500 parts of glucan and 5 parts of baohuoside I. The baohuoside I glucan inclusion compound can improve the bioavailability of baohuoside I, overcomes the defect of poor oral absorption in the existing baohuoside I application technology, can obviously improve the immunity of livestock, improves the smoothness and brightness of livestock fur, reduces the livestock skin inflammation, promotes the intestinal health of the livestock and has the value of practical popularization and application when being applied to the livestock.
Description
Technical Field
The invention relates to the field of livestock consumption products, and in particular relates to a baohuoside I-containing clathrate compound, a baohuoside I-containing composition, a preparation method and application thereof.
Background
Epimedium is a perennial herb, including berberidaceae plant Epimedium brevicornum Maxim, epimedium sagittatum Maxim, epimedium pubescens Maxim, epimedium wushanense T.S. Wushan, epimedium koreanum nakai, epimedium hirsutum nakai, etc., and has wide medicinal value, and is recorded in "pharmacopeia 2015 edition of Chinese beast". Baohuoside I is an in vivo active structure of herba Epimedii, and is named 3- (alpha-L-rhamnose pyranoxy) -4' -methoxy-5, 7-dihydroxy-8-propynyl flavone by International Union of Pure and Applied Chemistry (IUPAC). However, the oral absorption of baohuoside I is poor, and the bioavailability is low, so that the exertion of the effect of baohuoside I is limited.
With the continuous improvement of the social productivity level and the consumption level, the demand of people for livestock is continuously increased, and in order to meet the high standard of mass consumption, the demands for reducing the occurrence of various livestock diseases and improving the quality of livestock are required, so that the demand for the food function of the livestock is improved, but at present, no research report on food or feed additive which takes baohuoside I as an active ingredient and improves the quality of the livestock is reported.
Disclosure of Invention
In order to solve the problems, the invention provides a baohuoside I glucan inclusion compound which is prepared from the following raw materials in parts by weight:
20 to 500 parts of glucan and 5 parts of baohuoside I.
Further, the composition is prepared from the following raw materials in parts by weight:
95 parts of glucan and 5 parts of baohuoside I.
Further, the glucan is beta-1, 3/alpha-1, 3-glucan.
Further, the beta-1, 3/alpha-1, 3-glucan is prepared by taking sodium dihydrogen phosphate, potassium nitrate, magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate and sucrose as raw materials and adding agrobacterium pulcherrima ZX09 for fermentation.
The invention also provides a preparation method of the baohuoside I glucan inclusion compound, which comprises the following steps:
(1) Weighing the raw materials according to the proportion;
(2) Dissolving baohuoside I with ethanol to obtain baohuoside ethanol solution; adding water into dextran, stirring at 80-90 deg.C for 2-3 hr, adding baohuoside ethanol solution, stirring for 30-60min, and drying to obtain baohuoside I dextran clathrate.
Further, the concentration of the ethanol is 95 percent, mL/mL; the baohuoside concentration in the baohuoside ethanol solution is 5.0 percent, g/mL; the mass volume ratio of the glucan to the water is 1-5g: 100mL, preferably 1g:100mL.
The invention also provides a composition for improving the quality of livestock, which is a preparation prepared by taking the baohuoside I glucan inclusion compound as an active ingredient and adding auxiliary materials acceptable in medicines or foods; the preparation is an oral preparation; the oral preparation is granule, solution, powder or paste.
Further, the paste is prepared by mixing the following raw materials in parts by weight:
8-12 parts of L-carnitine substance, 1-3 parts of gamma-aminobutyric acid, 1-3 parts of antioxidant, 2-4 parts of cinnamon series derivative, 5-7 parts of flavonoid compound, 1-3 parts of lipophilic surfactant, 8-12 parts of glycerol monolaurate and 5-15 parts of baohuoside I glucan inclusion compound;
the L-carnitine substance comprises any one or more of L-carnitine, L-carnitine hydrochloride and L-carnitine tartrate, and L-carnitine is preferred;
the antioxidant comprises one or more of ethoxyquinoline, butyl hydroxyanisole, dibutyl hydroxytoluene, propyl gallate, tert-butyl hydroquinone, tea polyphenol, vitamin E, vitamin C, L-calcium ascorbate, L-sodium ascorbate, L-ascorbic acid-2-phosphate and L-ascorbic acid-6-palmitate, preferably tea polyphenol, vitamin E or vitamin C;
the cinnamyl series derivatives comprise one or more of cinnamyl alcohol, cinnamaldehyde, cinnamic acid, alpha-amyl cinnamaldehyde, glycolated cinnamaldehyde, o-methoxy cinnamaldehyde, alpha-hexyl cinnamaldehyde, alpha-amyl cinnamaldehyde, alpha-methyl cinnamaldehyde, p-methoxy cinnamaldehyde, alpha-butyl cinnamaldehyde, alpha-amyl cinnamaldehyde dimethyl acetal, methyl cinnamate, ethyl cinnamate, benzyl cinnamate, phenethyl cinnamate, cinnamyl cinnamate, ethyl hydrocinnamate, isobutyl cinnamate, 3-phenylpropyl cinnamate, isoamyl cinnamate, propyl cinnamate, allyl cinnamate, linalyl cinnamate, isopropyl cinnamate, cinnamyl acetate, cinnamyl isobutyrate, cinnamyl butyrate, cinnamyl isovalerate, cinnamyl propionate, cinnamyl formate, alpha-amyl cinnamyl acetate, alpha-amyl cinnamyl formate, alpha-amyl cinnamyl isovalerate, and any one or more of cinnamyl cinnamaldehyde;
the flavonoids compounds include one or more of alfalfa flavone, eucommia ulmoides flavone, soybean flavone and Ampelopsis grossedentata flavone, preferably soybean flavone;
the lipophilic surfactant comprises citric acid fatty acid glyceride.
The invention also provides a preparation method of the composition, which comprises the following steps:
mixing L-carnitine substance, gamma-aminobutyric acid and antioxidant to obtain powder A, heating and dissolving Cinnamomi series derivatives, flavonoids, lipophilic surfactant and monolaurin, mixing, adding powder A and baohuoside I dextran clathrate, and mixing.
The invention finally provides the baohuoside I glucan inclusion compound and application of the composition in preparing veterinary drugs or feeds or feed additives for improving the quality of livestock.
Furthermore, the veterinary drug, the feed or the feed additive is used for improving the immunity of livestock, improving the smoothness and brightness of fur of the livestock, reducing the skin inflammation of the livestock and promoting the intestinal health of the livestock.
Described in the invention "p"i.e., probability, reflecting the magnitude of the likelihood of an event occurring; statistics obtained by the significance test methodpGenerally, in the followingp<A statistical difference is found at 0.05,p<0.01 is that there is a significant statistical difference,p<0.001 is an extremely significant statistical difference; meaning that the probability that the difference between samples is due to sampling error is less than 0.05, 0.01, 0.001.
The baohuoside I glucan inclusion compound can improve bioavailability of baohuoside I, overcomes the defect of poor oral absorption in the existing baohuoside I application technology, can obviously improve immunity of livestock, improves smoothness and brightness of skin and hair of the livestock, reduces skin inflammation of the livestock, promotes intestinal health of the livestock and has practical popularization and application values when being applied to the livestock.
It will be apparent that various other modifications, substitutions and alterations can be made in the present invention without departing from the basic technical concept of the invention as described above, according to the common technical knowledge and common practice in the field.
The present invention will be described in further detail with reference to the following examples. This should not be understood as limiting the scope of the above-described subject matter of the present invention to the following examples. All the technologies realized based on the above contents of the present invention belong to the scope of the present invention.
Drawings
FIG. 1 is a graph of the effect of baohuoside I glucan on the growth performance of weaned pigs;
FIG. 2 is a graph of the results of the effect of baohuoside I glucan on the nutrient digestion and utilization of weaned pigs;
FIG. 3 is a graph showing the effect of baohuoside I glucan on the biochemical indicators of blood of weaned pigs;
FIG. 4 is a graph of the effect of baohuoside I glucan on weaned piglet leukocyte differential counts;
FIG. 5 is a graph of the results of the effect of baohuoside I glucan on the cellular immune function of weaned piglets;
FIG. 6 is a graph of the effect of baohuoside I glucan on the blood immunity index of weaned pigs;
FIG. 7 is a graph of the results of the effect of baohuoside I glucan on the small intestinal mucosal morphological structure of weaned piglets;
FIG. 8 is a graph of the results of the effect of baohuoside I glucan on the small intestinal mucosal morphology and structure of weaned piglets;
FIG. 9 is a graph of pet dog appearance statistics.
Detailed Description
The raw materials and equipment in the embodiment of the invention are obtained by commercial purchase, wherein the agrobacterium Prussonensis ZX09 (B)Agrobacterium pusense ZX 09) was discovered by the university of tokyo physicist, and filed for patent CN201010146371.2, which was assigned to sythetai, sikawa, new photobiotechnology limited, granted.
Example 1 preparation of baohuoside I glucan clathrate of the present invention
(1) Beta-1, 3/alpha-1, 3-glucan preparation
Adding 15-20L of water into a fermentation tank, adding 15-25g of sodium dihydrogen phosphate, 35-45g of potassium nitrate, 4-6g of magnesium sulfate, 0.1-0.2g of calcium chloride, 0.2-0.3g of ferrous sulfate, 0.05-0.10g of manganese sulfate, 35-45g of sodium macerate and 600g of cane sugar, dissolving and mixing uniformly, and adjusting the pH value to 7 by using a sodium hydroxide solution. Steam sterilizing at 110-130 deg.C for 30min, introducing sterile air, cooling the interlayer with cooling water, and cooling to obtain fermentation broth with total volume of 20-25L;
inoculating 200mL of agrobacterium putida ZX09 seed solution into a fermentation tank, adjusting the aeration flow to 20L/min, stirring at 250-300rpm, setting the fermentation temperature to 30 ℃, and culturing for 60h. After fermentation, putting the fermentation liquor into a barrel, adding 95% ethanol to precipitate the fermentation liquor, drying the precipitate by pressure, drying by using an air-blast drying oven, and crushing after drying to obtain a finished product of beta-1, 3/alpha-1, 3-glucan.
(2) Preparation of aqueous solution of beta-1, 3/alpha-1, 3-glucan
The beta-1, 3/alpha-1, 3-glucan is taken and mixed with water to form a beta-1, 3/alpha-1, 3-glucan aqueous solution with the concentration of 1.0 percent (0.01 g/mL).
(3) Preparation of baohuoside ethanol solution
Dissolving baohuoside I with 95% ethanol to obtain 5.0% (0.05 g/mL) baohuoside ethanol solution.
(4) Preparation of baohuoside I glucan inclusion compound
Stirring 1.0% (0.01 g/mL) of beta-1, 3/alpha-1, 3-glucan aqueous solution in a water bath at 80-90 ℃ for 2-3h, then adding 5.0% (0.05 g/mL) of baohuoside ethanol solution, wherein the volume ratio of the glucan aqueous solution to the baohuoside ethanol solution is 95. Cooling, pre-freezing the mixed solution in a refrigerator at-70 deg.C for 10-14 hr, freeze-drying in a vacuum freeze-drying machine for 16-20 hr, and pulverizing the dried product to obtain baohuoside I dextran clathrate.
Example 2 composition for improving poultry quality
The formula is as follows: 10 parts of L-carnitine, 2 parts of gamma-aminobutyric acid, 2 parts of vitamin C, 3 parts of cinnamaldehyde, 6 parts of daidzein, 2 parts of citric acid fatty glyceride, 10 parts of lauric acid monoglyceride, and 10 parts of baohuoside I glucan inclusion compound prepared in example 1;
the preparation method comprises the following steps: uniformly mixing L-carnitine, gamma-aminobutyric acid and vitamin C to obtain powder A; heating cinnamaldehyde, daidzein, citric acid fatty glyceride and lauric acid monoglyceride to 40-60 deg.C, dissolving, mixing, adding powder A and baohuoside I dextran clathrate, and mixing to obtain water-in-oil type poultry nutrition supplementing paste.
Example 3 composition for improving poultry quality
The formula is as follows: 8 parts of L-carnitine, 1 part of gamma-aminobutyric acid, 1 part of vitamin E, 2 parts of cinnamic acid, 5 parts of alfalfa flavone, 1 part of citric acid fatty glyceride, 8 parts of lauric acid monoglyceride, and 5 parts of baohuoside I glucan clathrate compound prepared in example 1;
the preparation method comprises the following steps: uniformly mixing L-carnitine, gamma-aminobutyric acid and vitamin E to obtain powder A; heating cinnamic acid, alfalfa flavone, citric acid fatty glyceride and lauric acid monoglyceride to 40-60 deg.C, dissolving, mixing, adding powder A and baohuoside I dextran clathrate, and mixing to obtain water-in-oil type poultry nutrition supplementing paste.
Example 4 composition for improving poultry quality
The formula is as follows: 12 parts of L-carnitine, 3 parts of gamma-aminobutyric acid, 3 parts of tea polyphenol, 4 parts of ethyl cinnamate, 7 parts of vine tea flavone, 3 parts of citric acid fatty glyceride, 12 parts of lauric acid monoglyceride, and 15 parts of baohuoside I glucan inclusion compound prepared in example 1;
the preparation method comprises the following steps: uniformly mixing L-carnitine, gamma-aminobutyric acid and tea polyphenol to obtain powder A; heating ethyl cinnamate, ampelopsis grossedentata flavone, citric acid fatty glyceride and lauric acid monoglyceride to 40-60 ℃, dissolving and mixing uniformly, adding the powder A and baohuoside I glucan inclusion compound, and mixing uniformly to obtain the water-in-oil type poultry nutrition supplement paste.
The advantageous effects of the present invention are described below by way of test examples.
Test example 1 effectiveness test of baohuoside I glucan clathrate in weaned pig
1. Purpose of the test
The application effect of the baohuoside I glucan inclusion compound prepared in example 1 on weaned piglets is preliminarily examined by examining the influence of the baohuoside I glucan inclusion compound on the production performance, nutrient digestion, intestinal health and immune function of the weaned piglets.
2. Test animals and test design
The test adopts single-factor test design. 108 healthy 21-day-old [ Du x (Long x big) ] weaned piglets were selected and randomly divided into 3 treatments (basal feed group, baohuoside I glucan mixture group and baohuoside I glucan inclusion group) according to the principle that the weights were close and half of the mothers and mothers, and each treatment was 6 replicates, and 6 piglets were replicated. The 7 days from the beginning of the test is a pre-feeding period, so that the piglets are adaptive to the environment and the feed. After the start of the formal test, each treated piglet was fed with the corresponding diet, and the period of the formal test was 28 days.
3. Experimental diet and feed management
The experimental basal diet was of the corn-soybean meal type, formulated with reference to NRC (2012) 7-11kg and 11-25kg stage swine nutritional needs and guidelines associated with the department of agriculture. The experimental diet is formed by respectively adding 5mg/kg baohuoside I, 95mg/kg beta-1, 3/alpha-1, 3-glucan mixture and 100mg/kg baohuoside I glucan inclusion compound into basic diet according to experimental design. All diets did not contain any antibiotics.
All experimental piglets were managed by conventional feeding. The experiment cleans and disinfects whole pig house, ensures the environment safety and sanitation of raising. During the test period, piglets eat and drink water freely, the temperature of the colony house is controlled to be 25-28 ℃, the humidity is not higher than 80%, the colony house is cleaned and disinfected regularly every day, and a plurality of disinfectants are alternately used, so that the bacterial drug resistance is reduced, and the ventilation, cleanness, dryness and sanitation of the colony house are kept. Piglet digestion trials were performed on days 24-27 of the trial.
4. Sample collection
(1) Feed sample: collecting each experimental diet sample according to a quartering method, and storing at-20 ℃ to be tested.
(2) Blood sample: after the experiment is finished, 1 piglet is randomly selected on an empty stomach to take 10mL of blood from the anterior vena cava, kept stand for 30min at low temperature, centrifuged at 3000r/min for 15min to prepare serum, and stored at-20 ℃ for later use.
(3) Tissue sample: at the end of the trial 1 piglet slaughter was selected at approximately the average body weight per replicate. After slaughtering, the abdominal cavity is opened quickly to separate intestinal tissues, empty tracts and ileum intestinal sections are collected, and intestinal contents are collected and stored at-80 ℃ for later use.
5. Index measurement
(1) Growth performance and health observation
During the test period, the health condition of the piglets is observed and recorded every day; piglets were weighed in replicates on an empty stomach at the beginning and end of the trial, and daily feed intake was recorded for calculation of Average Daily Feed Intake (ADFI), average Daily Gain (ADG) and feed-to-weight ratio (F/G) for each stage. Diarrhea rate = number of diarrhea piglets in trial period/(number of piglets x trial days) × 100%.
(2) Determination of nutrient level in feed raw material and feces
Respectively crushing the collected feed samples and the dried and remoistened manure samples, and sieving the crushed feed samples and the manure samples with a 40-mesh sieve, wherein the moisture, the energy, the crude protein and the crude ash in the feed and the manure samples are measured according to the method of feed analysis and feed quality detection technology.
(3) Measurement of blood biochemical indices
The total serum protein, albumin, urea nitrogen, blood sugar content, glutamic acid aminotransferase, aspartate transferase and alkaline phosphatase activity are measured by using the kit, and the operation is carried out according to the kit instructions.
(4) Differential blood leukocyte count
Differential blood leukocyte counts (neutrophils, lymphocytes, monocytes) were measured using a hematology analyzer.
(5) Peripheral lymphocyte subpopulation assay
Peripheral lymphocyte subpopulations (CD 3+, CD3+ CD4+, CD3+ CD8 +) were isolated using flow cytometry.
(6) Cytokine and humoral immunity index measurement
Serum IFN-gamma, IL-6, IL-12 and immune globulin IgG, igM were measured by ELISA kit of pig.
(7) Intestinal histomorphometry
Tissue sections of jejunum and ileum sections are prepared, HE staining is carried out, and the microscopic morphology (villus height, crypt depth and intestinal wall thickness) of the small intestine is observed under a microscope.
(8) Intestinal content flora detection
Extracting total bacteria DNA of jejunum and caecum chyme, and measuring the quantity of total bacteria, escherichia coli, bifidobacteria and lactobacillus by using RT-PCR technology.
6. Results and discussion
(1) Influence of baohuoside I glucan on growth performance of weaned pigs
As shown in fig. 1, the experimental group can significantly improve the growth performance of piglets. Compared with the basal diet group and the mixture group, the inclusion compound group obviously improves the average daily feed intake of piglets within 1-28 days (p= 0.05) and average daily gain: (p<0.05 And remarkably reduces the diarrhea rate of piglets: (p<0.05 And the feed meat ratio has no obvious difference in the whole period.
(2) Influence of baohuoside I glucan on digestion and utilization of weaned pig nutrients
As shown in FIG. 2, the test group was effectiveImprove the nutrient digestibility of the feed. The inclusion group significantly increased the feed dry matter, energy, crude protein, ash digestibility compared to the basal diet group and the mix group (p<0.05）。
(3) Influence of baohuoside I glucan on blood biochemical indexes of weaned pigs
As shown in fig. 3, the test group significantly affected serum total protein, urea nitrogen and glutamic-pyruvic transaminase activities. The inclusion group significantly increased the total serum protein content and significantly reduced the serum urea nitrogen content and alanine aminotransferase activity (as compared to the basal diet group and the cocktail group) (ii)p<0.05 Indicating that the body's ability to synthesize protein using amino acids is enhanced and the body's rapid growth is maintained.
(4) Influence of baohuoside I glucan on differential white blood cell count of weaned piglets
As shown in FIG. 4, the inclusion group had a significant effect on the numbers of neutrophils, monocytes and lymphocytes: (p<0.05). The leucocytes are used as a huge blood cell family, the neutrophils and the monocytes can phagocytose foreign matters to generate corresponding antibodies, the leucocytes play roles in curing collective injury and resisting pathogen invasion and disease immunity in organisms, and the lymphocytes can secrete and carry the antibodies to participate in cellular and humoral immunity.
As shown in fig. 5, the clathrate group had a significant effect on CD3+, CD4+ and CD8+ cell numbers: (p<0.05). CD4+ and CD8+ cells are the major lymphocytes that collectively maintain immune stability and balance, and their mutual restriction regulates the body's immune homeostasis. CD4+ increase indicates an increase in immunoglobulin production by B cells and an increase in cellular immunity, and CD8+ increase indicates immunosuppression, which can inhibit the production of antibodies by B cells and inhibit the killing function of killer T cells. The test result shows that the baohuoside I glucan inclusion compound can influence the conversion of peripheral T lymphocytes of piglets and regulate the immune system of organisms.
As shown in FIG. 6, the test group was able to regulate the blood IFN-. Gamma.content. IFN-gamma is an important immune regulator, and can make cell produce antiviral protein by cell surface receptor action so as to inhibit virus replication. This trialIn the experiment, the inclusion compound group has no obvious influence on the levels of the cytokines IL-6 and IL-12, but can obviously increase the level of IFN-gamma in blood (p<0.05 Shows that the baohuoside I glucan inclusion compound can improve the disease resistance of piglets to a certain extent. Compared with the control group and the mixture group, the inclusion compound group also obviously improves the IgM and IgG levels in blood, which indicates that the immune epidemic prevention mechanism of the organism starts to be started at the moment, and the immune level is improved to enhance the resistance.
(5) Influence of baohuoside I glucan on intestinal health of weaned pigs
As shown in fig. 7, the experimental group was able to induce significant histological changes in the ileal and jejunal mucosa. The inclusion compound group can obviously reduce the depth of ileum crypt and increase the villus height and the intestinal wall thickness of ileum: (p<0.05 ); and significantly increases jejunal crypt depth, villus height and intestinal wall thickness: (p<0.05). The test result shows that the baohuoside I glucan inclusion compound can influence the intestinal development by changing the morphological structures of ileum and jejunum mucous membranes, thereby improving the nutrient digestibility.
As shown in FIG. 8, the inclusion complex group significantly increased the number of bifidobacteria jejunum and cecal: (p<0.05 Increasing the number of Lactobacillus caecum: (p<0.05 And significantly reduce the number of cecal escherichia coli (c) ((b))p<0.05 ); but the test group has no significant influence on the total bacteria number of jejunum and cecum.
7. Conclusion
Under the test condition, compared with basic feed and a mixture of baohuoside I glucan added, the baohuoside I glucan inclusion compound added into the feed can improve the growth performance of piglets at the whole period of 1-28 days after weaning, obviously improve the nutrient digestibility of the feed, improve the immunity function of piglets, is favorable for maintaining the small intestine shape structure of the piglets, obviously improve the intestinal micro-ecological environment and promote the intestinal health of the piglets.
Test example 2 effectiveness test of baohuoside I glucan clathrate-containing composition in pet dogs
Dividing 200 pet dogs into two groups according to the principle of half each male and female, wherein the two groups are respectively an experimental group and a control group, and the difference of general data between the two groups has no statistical significance: (p>0.05 Comparable to each other). The pet dogs were fed 90 days without any additional addition of 150mg/kg of the composition prepared in example 2 to the pet food of the experimental group, and other nutritional supplements were not fed during the experiment, and the results of each group of pet dogs were observed and recorded, as shown in fig. 9.
As can be seen from FIG. 9, the skin condition, hair gloss, hair loss and tear marks of the pet dogs in the test groups are obviously improved compared with the control groups, and the composition containing baohuoside I glucan inclusion compound can ensure that the overall body state of the pet dogs is healthier and the pet dogs grow better.
In conclusion, the baohuoside I glucan inclusion compound can improve the bioavailability of baohuoside I, overcomes the defect of poor oral absorption in the existing baohuoside I application technology, can obviously improve the immunity of livestock, improves the smoothness and brightness of livestock fur, reduces the livestock skin inflammation, promotes the intestinal health of the livestock and has the value of practical popularization and application when being applied to the livestock.
Claims (7)
1. A baohuoside I glucan inclusion compound for improving the quality of livestock is characterized in that: the composition is prepared from the following raw materials in parts by weight:
95 parts of beta-1, 3/alpha-1, 3-glucan and 5 parts of baohuoside I;
the beta-1, 3/alpha-1, 3-glucan is prepared by taking sodium dihydrogen phosphate, potassium nitrate, magnesium sulfate, calcium chloride, ferrous sulfate, manganese sulfate and sucrose as raw materials and adding agrobacterium pulcherrima ZX09 for fermentation.
2. The process for preparing baohuoside I glucan clathrate compound of claim 1, wherein: it comprises the following steps:
(1) Weighing the raw materials according to the proportion of claim 1;
(2) Dissolving baohuoside I with ethanol to obtain baohuoside ethanol solution; adding water into dextran, stirring at 80-90 deg.C for 2-3 hr, adding baohuoside ethanol solution, stirring for 30-60min, and drying to obtain baohuoside I dextran clathrate.
3. The method of claim 2, wherein: the concentration of the ethanol is 95 percent, mL/mL; the baohuoside ethanol solution contains baohuoside with concentration of 5.0% and g/mL; the mass volume ratio of the glucan to the water is 1-5g: 100mL.
4. A composition for improving livestock quality, comprising: the baohuoside I glucan inclusion compound is a preparation prepared by taking the baohuoside I glucan inclusion compound as an active ingredient and adding auxiliary materials acceptable for medicines or foods; the preparation is an oral preparation; the oral preparation is granule, solution, powder or paste.
5. The composition of claim 4, wherein: the ointment is prepared by mixing the following raw materials in parts by weight:
8-12 parts of L-carnitine substance, 1-3 parts of gamma-aminobutyric acid, 1-3 parts of antioxidant, 2-4 parts of cinnamon series derivative, 5-7 parts of flavonoid compound, 1-3 parts of lipophilic surfactant, 8-12 parts of glycerol monolaurate and 5-15 parts of baohuoside I glucan clathrate compound of claim 1;
the L-carnitine substance comprises any one or more of L-carnitine, L-carnitine hydrochloride and L-carnitine tartrate;
the antioxidant comprises one or more of ethoxyquinoline, butyl hydroxyanisole, dibutyl hydroxytoluene, propyl gallate, tert-butyl hydroquinone, tea polyphenol, vitamin E, vitamin C, L-calcium ascorbate, L-sodium ascorbate, L-ascorbic acid-2-phosphate and L-ascorbic acid-6-palmitate;
the cinnamon series derivatives comprise one or more of cinnamyl alcohol, cinnamaldehyde, cinnamic acid, alpha-amyl cinnamyl alcohol, ethylene glycol cinnamaldehyde, o-methoxy cinnamaldehyde, alpha-hexyl cinnamaldehyde, alpha-amyl cinnamaldehyde, alpha-methyl cinnamaldehyde, p-methoxy cinnamaldehyde, alpha-butyl cinnamaldehyde, alpha-amyl cinnamaldehyde dimethyl acetal, methyl cinnamate, ethyl cinnamate, benzyl cinnamate, phenethylcinnamate, cinnamyl cinnamate, ethyl hydrocinnamate, isobutyl cinnamate, 3-phenylpropyl cinnamate, isoamyl cinnamate, propyl cinnamate, allyl cinnamate, linalyl cinnamate, isopropyl cinnamate, cinnamyl acetate, cinnamyl isobutyrate, cinnamyl butyrate, cinnamyl isovalerate, cinnamyl propionate, cinnamyl formate, alpha-amyl cinnamyl acetate, alpha-amyl cinnamyl formate, and alpha-amyl cinnamyl isovalerate;
the flavonoid compound comprises one or more of alfalfa flavone, eucommia ulmoides flavone, soybean flavone and vine tea flavone;
the lipophilic surfactant comprises citric acid fatty acid glyceride.
6. A method of preparing the composition of claim 4, wherein: it comprises the following steps:
mixing L-carnitine substance, gamma-aminobutyric acid and antioxidant to obtain powder A, heating and dissolving Cinnamomi series derivatives, flavonoids, lipophilic surfactant and monolaurin, mixing, adding powder A and baohuoside I dextran clathrate, and mixing.
7. Use of baohuoside I glucan clathrate according to claim 1 and composition according to claim 4 in the preparation of veterinary drugs or feed additives for improving livestock quality, characterized in that: the feed or the feed additive is a feed or a feed additive for improving the immunity of livestock and promoting the intestinal health of the livestock; the veterinary drug is a veterinary drug for promoting intestinal health of livestock.
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| CN101810254A (en) * | 2009-12-30 | 2010-08-25 | 沈阳科丰牧业科技有限公司 | Low-chitosan oligochitosan-epimedium extractive ecological feed additive |
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| CN101810254A (en) * | 2009-12-30 | 2010-08-25 | 沈阳科丰牧业科技有限公司 | Low-chitosan oligochitosan-epimedium extractive ecological feed additive |
| CN102144716A (en) * | 2010-02-08 | 2011-08-10 | 上海百树生物科技有限公司 | Herba epimedii extract feedstuff additive and preparation method thereof |
| CN103800916A (en) * | 2014-02-13 | 2014-05-21 | 重庆市中药研究院 | Glucan inclusion compound of water-insoluble small molecule drug and preparation method thereof |
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