KR20050069719A - Insecticide composition containing tephrosia extract - Google Patents
Insecticide composition containing tephrosia extract Download PDFInfo
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- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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Abstract
본 발명은 천연 살충 조성물에 관한 것으로서, 더욱 상세하게는 테프로시아 추출물이 함유되어 있어 각종 농작물 저해 해충 및 파리, 모기와 같은 해충에 대한 방제효과가 우수하다.The present invention relates to a natural insecticidal composition, and more particularly, it contains an extract of teprocia, which is excellent in controlling pests such as various crops inhibiting pests and flies and mosquitoes.
Description
본 발명은 천연 살충 조성물에 관한 것으로서, 더욱 상세하게는 테프로시아(Tephrosia purpurea) 추출물을 함유하여 각종 해충에 대한 방제효과가 우수하고 특히 응애, 배추 좀나방과 같은 난방제 해충에 대한 방제효과가 탁월할 뿐만 아니라 인축에는 전해 무해한 천연 살충 조성물에 관한 것이다.The present invention relates to a natural insecticidal composition, and more particularly, it contains Tephrosia purpurea extract, which is excellent for controlling various pests, and in particular, for controlling insect pests such as mites and Chinese cabbage moths. Not only is it excellent, it relates to a natural pesticidal composition that is electrolytically harmless to human beings.
오늘날, 각종 유기합성 농약의 대량 살포에 따른 토양 및 수질 오염 등의 환경 공해가 문제시되고 있으며, 이에 따라 저독성이거나 혹은 무공해성의 천연 농약에 대한 연구가 활발히 진행되고 있다. 또한, 모기, 파리 등의 해충을 제거하기 위한 살충제로 여러가지 살충제가 사용되고 있으나, 독성이 강하고 환경오염을 일으키며 일시적인 살충효과는 나타나더라도 내성이 생겨 전체 개채수가 오히려 증가하는 기현상마저 나타나고 있는 실정이다. 이러한 부작용으로 인해 뉴질랜드, 호주 등지에서는 살충제의 사용이 법으로 금지되어 있다. Today, environmental pollution such as soil and water pollution caused by the large-scale spraying of various organic synthetic pesticides is a problem, accordingly, research on natural pesticides with low toxicity or no pollution is being actively conducted. In addition, various insecticides are used as insecticides to remove pests such as mosquitoes and flies, but even if the toxicity is strong, causing environmental pollution, and even if a temporary insecticidal effect occurs, resistance is generated and even the overall number of dogs is increasing. Due to these side effects, pesticides are prohibited by law in New Zealand and Australia.
따라서, 본 발명의 목적은 광범위한 살충 스펙트럼을 나타낼 뿐만 아니라 인축에 독성이 없는 천연 살충제 조성물을 제공하는 것이다. Accordingly, it is an object of the present invention to provide natural insecticide compositions that exhibit a broad spectrum of pesticidal spectrum and are not toxic to shrinkage.
본 발명은 테프로시아(Tephrosia purpurea) 추출물을 유효량 함유하는 천연 살충 조성물을 그 특징으로 한다.The present invention is characterized by a natural insecticidal composition containing an effective amount of Tephrosia purpurea extract.
본 발명이 원료로 사용하는 천연식물의 특성을 간략하게 요약하면 다음과 같다.Briefly summarized the characteristics of the natural plant used as a raw material of the present invention are as follows.
테프로시아(Tephrosia purpurea) 는 어독성을 나타내는 식물로 잘 알려져 있다. 테프로시아는 테프로신(tephrosin)이라는 물고기를 화학적으로 기절시키는 아주 약한 독을 가지고 있으며 이 독은 포유류에는 영향을 주지 않는다. 테프로시아 나무에서 어독 성분을 얻는 방법은 매우 간단하여 절구나 막자사발등에 테프로시아 가지를 꺾어 넣고 갈아서 얻게 된다. Tephrosia purpurea is well known for its fish toxicity. Teprosia has a very weak poison that chemically stuns a fish called tephrosin, which does not affect mammals. The method of obtaining the poisonous ingredient from the Tepprosia tree is very simple, and it is obtained by cutting and cutting the Teponia eggplant into the mortar or mortar.
본 발명의 테프로시아 추출물은 통상의 극성 유기용매를 사용하여 추출하였는 바, 대표적으로는 메탄올, 에탄올, 이소프로판올, n-프로판올, 벤질알콜, 프로필 셀룰로스, 감마-부틸로락톤, N-메틸-2-피롤리돈, 메틸 나프탈렌, 지방산 메틸 에스터 등 통상적인 용매들이 단독 혹은 혼합 사용이 가능하다. 그러나 용매의 선정은 테프로시아에 포함된 유효살충 성분의 추출효율에 지대한 영향을 미치므로 주의 깊게 산정하여 사용한다. 또한, 추출 시간과 온도는 유효활성 성분의 열분해가 없는 80℃ 이하의 온도에서 5-10 시간 추출, 여과, 농축 한다. 본 발명에서 사용한 추출 방법은 전통적 방법에 따른 것으로 충분하였다.Teprocia extract of the present invention was extracted using a conventional polar organic solvent, typically methanol, ethanol, isopropanol, n-propanol, benzyl alcohol, propyl cellulose, gamma-butyrolactone, N-methyl-2 Conventional solvents such as pyrrolidone, methyl naphthalene, fatty acid methyl ester can be used alone or in combination. However, the choice of solvent has a great influence on the extraction efficiency of the effective pesticides contained in Tepsia, so it should be carefully calculated and used. In addition, the extraction time and temperature is extracted, filtered and concentrated for 5-10 hours at a temperature of 80 ° C. or less without pyrolysis of the active ingredient. The extraction method used in the present invention was sufficient according to the traditional method.
한편, 천연식물로부터 얻어지는 생약의 경우 그 원산지, 채취시기, 보관기간 및 보관상태에 따라 그 유효 활성성분의 함량이 크게 달라지는 바, 각각의 추출물에 대해서는 지표물질을 선정하고 이들 지표물질의 함량으로서 조성비를 한정하는 것이 보다 바람직하다. 본 발명에서는 테프로시아 지표물질로서 로테논(rotenone)을 선정하였다.On the other hand, in the case of herbal medicines obtained from natural plants, the content of the active ingredient varies greatly depending on the origin, collection time, storage period and storage condition. For each extract, the indicator material is selected and the composition ratio is used as the content of the indicator material. It is more preferable to limit. In the present invention, rotenone was selected as the indicator of teprocia.
따라서, 본 발명의 천연 살충 조성물은 지표물질로서 그 조성을 한정할 수 있는 바, 본 발명에 따른 천연 살충 조성물은 전체 조성물 100 g을 기준으로 로테논 0.015 ∼ 5 g을 포함할 수 있다. 본 발명의 천연 살충 조성물을 구성함에 있어, 유효 활성성분의 함량비가 0.015% 미만인 경우 다양한 해충에 대해 충분한 살충효과를 나타낼 수 없다. 유효성분의 함량이 많을수록 살충효과가 향상되나 5% 이상이 되면 함량 증가에 따른 효과 향상의 속도가 둔해지고, 경제성과 제형 제조의 용이성 등을 고려하여 0.015-5% 가 바람직하다. Therefore, the natural pesticidal composition of the present invention may limit its composition as an indicator material, and the natural pesticidal composition according to the present invention may include 0.015 to 5 g of rotenone based on 100 g of the total composition. In constructing the natural pesticidal composition of the present invention, when the content ratio of the active ingredient is less than 0.015%, it may not exhibit sufficient pesticidal effect against various pests. Increasing the content of the active ingredient is improved insecticidal effect, but if more than 5% is slowed the speed of effect improvement by increasing the content, considering the economical and ease of formulation formulation is preferably 0.015-5%.
이상에서 설명한 바와 같은 본 발명은 다음의 제조예에 의거하여 천연식물 추출물을 얻었고, 또한 다음의 실시예에 의거하여 천연 살충 조성물을 제조하였으며, 얻어진 각 실시예의 천연 살충 조성물에 대해서는 실험예의 방법으로 살충활성을 측정하였다. 또한, 다음의 제조예 및 실시예는 일 구현예로서 본 발명이 이러한 제조예 및 실시예에 의해 한정되는 것은 아니다.According to the present invention as described above, natural plant extracts were obtained according to the following preparation examples, and natural insecticidal compositions were prepared according to the following examples. Activity was measured. In addition, the following Preparation Examples and Examples as an embodiment, the present invention is not limited by these Preparation Examples and Examples.
제조예 1 : 프로테시아 추출물의 제조Preparation Example 1 Preparation of Proteacia Extract
프로테시아 나뭇잎을 2.0 ∼ 4.0 cm 정도로 세절하였다. 세절된 프로테시아 1 kg에 3L의 메탄올을 첨가한 후 믹서기로 완전히 분쇄한 다음 중탕기로 50도의 온도에서 8시간동안 끓인 후 잔류물을 필터로 거른 후 다시 가온하여 농축시켰다. The protea leaves were cut to about 2.0 to 4.0 cm. 3 L of methanol was added to 1 kg of shredded proteacia, which was then pulverized thoroughly with a blender, boiled at 50 ° C. for 8 hours in a water bath, and the residue was filtered off and concentrated by heating again.
참고예 : 지표물질의 선정Reference Example: Selection of Indicators
프로테시아 추출물의 지표물질인 로테논 화합물에 대한 HPLC 분석에는 Waters사의 용매 구배 펌프(gradient pump)를 사용하였으며 용매로는 0.1N 인산 수용액 / 아세토니트릴 용매를 초기 비율 90:10 비율에서 시작하여 90분까지 0:100 비율에 이르도록 구배시켜 적용하였다. 컬럼은 Phenomenex C18 이었고, 자외선 검출기는 295 nm에서 측정하였다. 시료는 오토 샘플러 통하여 10 마이크로씩 주입하였다. 결과는 동사의 Autochro software에서 분석 처리하였다. HPLC analysis of rotenone compounds, an indicator of proteacia extract, was carried out using Waters' solvent gradient pump.The solvents were 0.1N aqueous solution of phosphate / acetonitrile starting at 90:10 ratio and starting at 90:10. Gradients were applied to the 0: 100 ratio by minutes. The column was Phenomenex C18 and the ultraviolet detector was measured at 295 nm. Samples were injected 10 microns each through an auto sampler. The results were analyzed by the company's Autochro software.
이상의 방법으로 선정된 지표물질은 다음과 같다.Indicator substances selected by the above method are as follows.
화학식 1Formula 1
로테논은 녹는점이 163℃인 무색의 결정체이고, 포유동물에 대해서는, 50％ 치사량은 LD=350-1500㎎/㎏(집쥐의 구경투여)으로 나타났다.Rotenone is colorless crystals with a melting point of 163 ° C. For mammals, 50% lethal dose was LD = 350-1500 mg / kg (caliber administration of mice).
실시예 : 천연 살충 조성물의 제조Example: Preparation of Natural Insecticidal Compositions
상기 제조예의 방법으로 얻어진 각각의 추출물을 유화제 및 용매와 혼합하여 천연 살충 조성물을 제조하였다. 이때, 조성물의 균일한 유화를 위하여 표준 유화제로서 폴리옥시에틸렌 솔비탄 모노 올레이트(Koremul SFT-85 한농화성)와 폴리옥시에틸렌 옥틸 페닐 에테르(Koremul OP-10 한농화성)을 사용하였다. 용제로는 에탄올을 사용하였다. 조성물의 로테논 주성분의 함량을 3%로 조절하였다. 이 농약을 100배(농도 0.03%), 200배(0.015%), 400배(0.0075%), 800배(0.00375%)로 희석하였다. Each extract obtained by the method of Preparation Example was mixed with an emulsifier and a solvent to prepare a natural pesticidal composition. At this time, polyoxyethylene sorbitan monooleate (Koremul SFT-85 concentrating) and polyoxyethylene octyl phenyl ether (Koremul OP-10 concentrating) were used as standard emulsifiers for uniform emulsification of the composition. Ethanol was used as a solvent. The content of the rotenone active ingredient of the composition was adjusted to 3%. The pesticides were diluted 100 times (concentration 0.03%), 200 times (0.015%), 400 times (0.0075%) and 800 times (0.00375%).
표 1Table 1
실험예 1Experimental Example 1
상기 표 1의 조성으로 제조된 천연 살충 조성물의 약효작용에 대한 활성을 비교하기 위하여 다음과 같은 실험을 실시하였다.In order to compare the activity of the natural pesticidal composition prepared in the composition of Table 1 for the efficacy of the following experiment was carried out.
지름이 5 cm인 양배추잎을 준비된 약액에 30 초간 담구었다가 꺼내어 후드내에서 음건시킨 후 배추좀나방(DBM; Diamond-back moth, plutella xylostella)의 3령 유충을 20 마리씩 접종하여 24 시간과 48 시간 경과후에 각각 살충률을 측정하여 다음 표 2에 나타내었다.Soak cabbage leaves 5 cm in diameter for 30 seconds in the prepared solution, and then dry in the hood, and inoculate 20 3 larvae of Chinese cabbage moth (DBM; Diamond-back moth, plutella xylostella) each for 24 hours and 48 After the passage of time, the insecticidal rate was measured and shown in Table 2 below.
표 2TABLE 2
배추좀나방에 대한 약효 시험결과Drug Test Results for Chinese Cabbage Moth
실험예 2Experimental Example 2
진드기 30개체를 각각 시험을 위하여 1X1X1m인 가제로 싼 상자에 넣고, 상기 실시예 2와 실시예5의 제품을 분무하였다. 5,10,20분 후, 그리고 24시간 경과후의 살충률을 측정하여 다음 표 3 에 나타내었다. Thirty individual ticks were placed in boxes packed with a gauze of 1 × 1 × 1 m for testing, respectively, and the products of Examples 2 and 5 were sprayed. After 5, 10, 20 minutes, and after 24 hours the pesticidal rate was measured and shown in Table 3.
표 3TABLE 3
진드기에 대한 약효 시험결과 (살충율%)Drug test results for ticks (% insecticide)
이상에서 설명한 바와 같이, 본 발명의 천연 살충 조성물은 생약 추출물로 구성되어 있어 인축에 전혀 무해하면서도 각종 농작물 저해 해충 및 파리, 모기와같은 다양한 해충에 대한 방제효과가 우수하다. As described above, the natural insecticidal composition of the present invention is composed of herbal extracts, which are completely harmless to human beings, and are excellent in controlling various pests such as pests and flies and mosquitoes.
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| KR1020030102080A KR20050069719A (en) | 2003-12-31 | 2003-12-31 | Insecticide composition containing tephrosia extract |
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| KR20050069719A true KR20050069719A (en) | 2005-07-05 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103044440A (en) * | 2012-12-31 | 2013-04-17 | 深圳市华农生物工程有限公司 | Critical extraction method for rotenone |
| CN103651598A (en) * | 2013-12-30 | 2014-03-26 | 成都新朝阳作物科学有限公司 | Pesticide containing Annona reticulata and derris elliptica extracts, and preparation method and application thereof |
-
2003
- 2003-12-31 KR KR1020030102080A patent/KR20050069719A/en not_active Application Discontinuation
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103044440A (en) * | 2012-12-31 | 2013-04-17 | 深圳市华农生物工程有限公司 | Critical extraction method for rotenone |
| CN103651598A (en) * | 2013-12-30 | 2014-03-26 | 成都新朝阳作物科学有限公司 | Pesticide containing Annona reticulata and derris elliptica extracts, and preparation method and application thereof |
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