KR20050051619A - The manufacturing method of dried citrus unshiu peel tea using organic citrus unshiu - Google Patents

The manufacturing method of dried citrus unshiu peel tea using organic citrus unshiu Download PDF

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KR20050051619A
KR20050051619A KR1020050038866A KR20050038866A KR20050051619A KR 20050051619 A KR20050051619 A KR 20050051619A KR 1020050038866 A KR1020050038866 A KR 1020050038866A KR 20050038866 A KR20050038866 A KR 20050038866A KR 20050051619 A KR20050051619 A KR 20050051619A
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dermis
tea
organic
citrus
particles
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KR1020050038866A
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Korean (ko)
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KR100665016B1 (en
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김동일
허담
유경미
김채은
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동우당제약(주)
허담
김동일
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/06Treating tea before extraction; Preparations produced thereby
    • A23F3/14Tea preparations, e.g. using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/06Treating tea before extraction; Preparations produced thereby
    • A23F3/12Rolling or shredding tea leaves
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/16Tea extraction; Tea extracts; Treating tea extract; Making instant tea
    • A23F3/163Liquid or semi-liquid tea extract preparations, e.g. gels, liquid extracts in solid capsules
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/16Tea extraction; Tea extracts; Treating tea extract; Making instant tea
    • A23F3/22Drying or concentrating tea extract
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/34Tea substitutes, e.g. matè; Extracts or infusions thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/40Tea flavour; Tea oil; Flavouring of tea or tea extract

Abstract

본 발명은 유기농 감귤을 이용한 진피차의 제조 방법에 관한 것으로, 감귤의 과피를 반건조 한 진피를 일정 크기 이하로 분쇄하고, 이를 그 크기에 따라 다관용 및 티백용으로 분류하며, 각각 열을 가해 풍미를 더하는 로스팅 공정과 적절한 포장으로 진피차를 제조하여, 이 진피차를 방법에 따라 고온의 물에 우려내어 마심에 따라, 건강에 좋고, 맛과 향이 우수한 진피차를 용이하게 마실 수 있는 것이다.The present invention relates to a method of manufacturing dermis tea using organic citrus fruits, and to crush the semi-dried dermis of the tangerine's rind to a certain size or less, and classify it according to its size into a multi-use and tea bag, and apply heat to each flavor The dermis tea is prepared by a roasting process and appropriate packaging, adding the dermis tea to hot water according to the method, and drinking the tea with good taste and aroma.

본 발명의 제조 방법을 도1의 공정도를 참조하여 대략 설명하면, 먼저, 유기농으로 재배된 감귤을 선별하고, 이를 깨끗이 씻어 물기를 없애는 감귤의 세척 및 건조 공정(S1), 상기 감귤의 껍질에서 과육 부분을 분리하여 과피를 모으는 감귤의 과육 및 과피 분리 공정(S2), 분리된 과피를 반건조하는 과피 건조 공정(S3), 건조된 과피인 진피를 0.8㎜ 이하로 분쇄하는 진피 분쇄 공정(S4), 분쇄된 진피를 그 크기에 맞게 다관용과 티백용으로 분리하는 진피 입자 분류 공정(S5), 분류된 진피 입자를 각각 열가공하는 진피 입자 로스팅 공정(S6), 마지막으로, 로스팅 공정을 거친 진피 입자를 다관용 및 티백용과 같이 진피차를 먹는 방법에 맞게 적절히 포장하는 진피차 포장 공장(S7)의 제조 방법으로 진피차를 완성한다.Referring to the manufacturing method of the present invention with reference to the process diagram of Figure 1, first, first, the organically grown citrus, and washing and drying the tangerine to remove water by washing it clean (S1), pulp in the peel of the tangerine Citrus pulp and skin separation process (S2), which separates the parts and collects the skin, skin drying process (S3), semi-drying the separated skin, and dermis grinding process (S4), which pulverizes the dried dermis to 0.8 mm or less. , Dermis particle sorting process (S5) for separating the pulverized dermis into multi-tubes and tea bags according to its size, dermal particle roasting process (S6) for thermally processing the sorted dermis particles, respectively, and finally, the dermis having been roasted Dermal tea is completed by the manufacturing method of the dermis tea packaging factory (S7) that the particles are properly packaged according to the method of eating dermis tea, such as for tea and tea bags.

이와 같이 유기농 감귤을 이용한 다관용 및 티백용 진피차는 대략 80℃ 정도의 물에 우려내어, 기호에 따라 꿀이나, 설탕 등을 첨가하여 마실 수 있음에 따라, 맛과 향이 우수한 진피차의 음용이 간단하고, 비타민 C 등의 파괴를 최소화하며, 피로회복 및 감기예방에 좋고, 농약, 왁스와 같은 성분이 포함되지 않은 유기농 감귤을 사용하므로 인체에 해로운 화학 성분이 체내에 축적되지 않으며, 보관이 용이한 특징이 있다.As such, the dermis tea for tea and tea bags using organic citrus can be drunk with water of about 80 ℃, and can be drunk with honey or sugar according to your taste. Minimizes the destruction of vitamins, vitamins, etc. It is good for fatigue and cold prevention, and organic tangerines that do not contain ingredients such as pesticides and waxes do not accumulate harmful chemicals in the body and are easy to store. There is this.

Description

유기농 감귤을 이용한 진피차의 제조 방법 { The manufacturing method of dried citrus unshiu peel tea using organic citrus unshiu }{The manufacturing method of dried citrus unshiu peel tea using organic citrus unshiu}

본 발명은 유기농 감귤을 이용한 진피차의 제조 방법에 관한 것으로, 감귤의 과피를 반건조 한 진피를 일정 크기 이하로 분쇄하고, 이를 그 크기에 따라 다관용 및 티백용으로 분류하며, 각각 열을 가해 풍미를 더하는 로스팅 공정과 적절한 포장으로 진피차를 제조하여, 이 진피차를 방법에 따라 고온의 물에 우려내어 마심에 따라, 건강에 좋고, 맛과 향이 우수한 진피차를 용이하게 마실 수 있는 것이다.The present invention relates to a method of manufacturing dermis tea using organic citrus fruits, and to crush the semi-dried dermis of the tangerine's rind to a certain size or less, and classify it according to its size into a multi-use and tea bag, and apply heat to each flavor The dermis tea is prepared by a roasting process and appropriate packaging, adding the dermis tea to hot water according to the method, and drinking the tea with good taste and aroma.

일반적으로 진피라 함은 감귤의 껍질을 건조한 것으로, 이는 감귤의 과육보다 많은 비타민 C가 들어 있어, 피로회복과 감기예방, 피부미용에 좋고, 칼슘이 체내에 흡수되도록 도와주며, 또, 진피에 포함된 비타민 P는 과일류 중에서 감귤에만 함유된 성분으로, 비타민 C의 작용을 더욱 강화시키고, 모세혈관을 보호하기 때문에 고혈압과 동맥경화를 예방하며, 이 밖에도 진피는 음식을 먹고 토하거나 설사를 할 때, 속이 더부룩하고 얹혔을 때에도 치료제로 사용되는데, 이는 그대로 달여 차로 먹거나, 다른 약재와 혼합하여 달여 먹고, 또는, 그 향이 좋아서 입욕제로도 사용되어, 몸을 상쾌하게 하고, 몸을 보온한다.Generally, the dermis is dried citrus peel, which contains more vitamin C than citrus pulp, which is good for fatigue recovery, cold prevention, skin care, and helps calcium absorb into the body. Vitamin P is a fruit-only ingredient in citrus fruits, which enhances the action of vitamin C and protects capillaries, preventing high blood pressure and arteriosclerosis, and the dermis when eating, vomiting, or diarrhea. It is also used as a remedy even when the stomach is simmered and topped, which is eaten as a tea as a decoction, mixed with other medicines, decocted, or used as a bath because of its fragrance, which refreshes the body and keeps the body warm.

그러나 상기와 같은 효과에도, 통상 방법으로 재배된 감귤은 병충해 방지를 위한 농약을 치고, 신선도를 유지하기 위해 피막제를 바르며, 왁스를 발라 보기 좋게 하므로, 진피를 만들기 위해서 감귤의 껍질을 그대로 사용하기보다는, 과피로 분리하기 전 감귤을 깨끗이 씻어, 과육을 분리해낸 과피를 썩지 않게 건조하는 등의 관리가 필요하며, 이 진피를 차로 마실 때에는, 진피가 건조된 상태이므로, 오래 끊여 우러나오게 해야하므로 차 마시기가 간단하지 않을뿐더러, 오래 달이면서 비타민 C가 파괴되는 문제점이 있으며, 진피의 용량을 적정하게 맞추지 못하면, 너무 연하거나, 향이 진해 마시기 곤란한 문제점이 있었다. However, despite the above effects, citrus cultivated by the conventional method is applied to the pesticides to prevent pests, to coat the film to maintain freshness, and to apply wax to make it look better, rather than using the peel of citrus to make the dermis In addition, care should be taken to clean the tangerines before they are separated into skins, and to dry the skins from which the flesh is separated, to avoid decay.When drinking this dermis with tea, since the dermis is dry, it must be cut off for a long time to drink tea. Not only is not simple, and there is a problem that vitamin C is destroyed for a long time, and if the dose of the dermis is not properly adjusted, it is too soft, or the fragrance is difficult to drink.

따라서 본 발명은 상기의 문제점을 해결하고자, 유기농 감귤의 과피를 반건조한 진피를 일정 크기 이하로 분쇄하고, 이를 그 크기에 따라 다관용 및 티백용으로 분류하며, 이 분류된 진피 입자에 열을 가해 풍미를 더하는 로스팅 공정과, 적절한 포장을 거쳐 진피차를 제조하여, 이를 온도가 높은 물에 우려내어 맛과 향이 우수한 진피차를 용이하게 마실 수 있음에 따라, 비타민 C의 파괴를 최소화하고, 피로회복과 감기예방, 피부미용에 좋고, 보관이 용이한 진피차를 제공하는데 그 목적이 있다.Therefore, the present invention, in order to solve the above problems, crush the semi-dried dermis of organic tangerine peel to a certain size or less, and classify it according to the size for multi-tolerance and tea bags, by applying heat to the classified dermis particles The dermis tea can be manufactured through a roasting process that adds flavor and proper packaging, and it can be easily consumed with a high taste and aroma because it is soaked in high temperature water to minimize the breakdown of vitamin C and to recover from fatigue and cold. The purpose of the present invention is to provide a dermis tea that is good for prevention and skin beauty and easy to store.

본 발명은 유기농 감귤을 이용한 진피차의 제조 방법에 관한 것으로, 감귤의 과피를 반건조 한 진피를 일정 크기 이하로 분쇄하고, 이를 그 크기에 따라 다관용 및 티백용으로 분류하며, 각각 열을 가해 풍미를 더하는 로스팅 공정과 적절한 포장으로 진피차를 제조하여, 이 진피차를 방법에 따라 고온의 물에 우려내어 마심에 따라, 건강에 좋고, 맛과 향이 우수한 진피차를 용이하게 마실 수 있는 것이다.The present invention relates to a method of manufacturing dermis tea using organic citrus fruits, and to crush the semi-dried dermis of the tangerine's rind to a certain size or less, and classify it according to its size into a multi-use and tea bag, and apply heat to each flavor The dermis tea is prepared by a roasting process and appropriate packaging, adding the dermis tea to hot water according to the method, and drinking the tea with good taste and aroma.

본 발명의 제조 방법을 도1의 공정도를 참조하여 대략 설명하면, 먼저, 유기농으로 재배된 감귤을 선별하고, 이를 깨끗이 씻어 물기를 없애는 감귤의 세척 및 건조 공정(S1), 상기 감귤의 껍질에서 과육 부분을 분리하여 과피를 모으는 감귤의 과육 및 과피 분리 공정(S2), 분리된 과피를 반건조하는 과피 건조 공정(S3), 건조된 과피인 진피를 0.8㎜ 이하로 분쇄하는 진피 분쇄 공정(S4), 분쇄된 진피를 그 크기에 맞게 다관용과 티백용으로 분리하는 진피 입자 분류 공정(S5), 분류된 진피 입자를 각각 열가공하는 진피 입자 로스팅 공정(S6), 마지막으로, 로스팅 공정을 거친 진피 입자를 다관용 및 티백용과 같이 진피차를 먹는 방법에 맞게 적절히 포장하는 진피차 포장 공장(S7)의 제조 방법으로 진피차를 완성한다.Referring to the manufacturing method of the present invention with reference to the process diagram of Figure 1, first, first, the organically grown citrus, and washing and drying the tangerine to remove water by washing it clean (S1), pulp in the peel of the tangerine Citrus pulp and skin separation process (S2), which separates the parts and collects the skin, skin drying process (S3), semi-drying the separated skin, and dermis grinding process (S4), which pulverizes the dried dermis to 0.8 mm or less. , Dermis particle sorting process (S5) for separating the pulverized dermis into multi-tubes and tea bags according to its size, dermal particle roasting process (S6) for thermally processing the sorted dermis particles, respectively, and finally, the dermis having been roasted Dermal tea is completed by the manufacturing method of the dermis tea packaging factory (S7) that the particles are properly packaged according to the method of eating dermis tea, such as for tea and tea bags.

본 발명에 따른 진피차의 제조 방법을, 도1의 공정도에 따라 순서대로 설명하면, 먼저 감귤의 세척 및 건조 공정(S1)은, 선별된 유기농 감귤을 깨끗하게 씻어, 외피에 부착된 먼지를 완전히 제거하고, 이를 닦거나 바람을 불어 건조하는 것이며, 감귤의 과육 및 과피 분리 공정(S2)은, 상기 깨끗하게 건조된 감귤에서 과육을 분리하고 과피, 즉, 감귤피를 모으는 것으로, 수작업 또는, 기계를 이용한 작업이 가능하다.DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS A method for manufacturing dermis tea according to the present invention will be described in order according to the process diagram of FIG. 1. First, the washing and drying process (S1) of citrus fruits is performed by thoroughly washing selected organic citrus fruits to completely remove dust attached to the skin. To clean, or blow and blow dry, the fruit tangerine and peeling process (S2) of separating the flesh from the clean dried citrus and collect the peel, that is, citrus peel, by hand or by machine This is possible.

다음으로, 과피 건조 공정(S3)은, 상기 분리한 과피를 햇빛에 자연 건조하는 것으로, 완전히 건조하는 것보다는 반건조하는 것이 바람직한데, 과피를 햇빛에 두고 건조하는 이유는 그늘에서 하는 것보다는 맛과 향이 더 우수하기 때문이며, 반건조하는 이유는 후설 할 분쇄 과정에서 적절한 크기의 입자를 얻고, 로스팅 과정에서 열을 받았을 때, 겉만 쉽게 타서 버리는 손실률을 낮추기 위함이며, 이 과피 건조 공정(S3)으로 감귤피를 건조한 진피를 얻는다.Next, the skin drying step (S3), the dried skin is naturally dried in the sunlight, it is preferable to semi-dry rather than completely dried, the reason for drying the skin in the sunlight is more delicious than the shade This is because the fragrance is better, and the reason for semi-drying is to obtain particles of the appropriate size in the grinding process to be sedimented, and to reduce the loss rate that is easily burned away when heated in the roasting process. Citrus peels get dry dermis.

또한, 진피 분쇄 공정(S4)은, 상기 건조된 과피, 즉 진피를 0.8㎜ 이하로 분쇄하는 공정으로, 진피 입자가 0.8㎜보다 클 경우에는, 맛과 향이 연하여 식감이 떨어지고, 기호성을 높이기 위해 많은 양이 소비되므로 경제적이지 못하며, 다음 진피 입자 분류 공정(S5)은, 상기 분쇄된 진피 입자를 그 굵기에 따라, 다관용과 티백용으로 분리 및 선별하는 것으로, 상기 다관용은 진피 입자를 0.5 내지 0.8㎜의 굵기로 선별하여, 다관을 이용한 찻물을 우려낼 때, 거름망에서 빠져나오지 않도록 하고, 티백용은 진피 입자를 0.3㎜ 이하의 굵기로 사용하여 진피차의 기호성을 높인다. In addition, the dermis grinding step (S4) is a step of grinding the dried skin, that is, the dermis to 0.8 mm or less, when the dermal particles are larger than 0.8 mm, the taste and aroma are soft, the texture is poor, to improve palatability It is not economical because a large amount is consumed, and the following dermal particle sorting process (S5) separates and selects the pulverized dermal particles according to their thickness, for multi-use and tea bags, and the multi-use for removing dermis particles 0.5 Selected to a thickness of 0.8 mm to prevent tea from using a multi-tube, so as not to escape from the strainer, for tea bags to increase the palatability of the dermis by using the dermis particles in a thickness of 0.3 mm or less.

이은 진피 입자 로스팅 공정(S6)은, 선별된 진피 입자를 열을 가해 향과 기호성을 높이는 것으로, 이는 고온의 열로 일시에 가열할 경우, 진피 입자의 내부가 로스팅되기 전에, 표면이 타버려 기호성이 떨어지고, 버려지는 손실률이 높아지므로, 로스팅의 열원을 할로겐 라이트 히터를 사용하면, 진피 입자의 내·외부를 골고루 로스팅하여 맛과 향이 증대할 뿐만 아니라, 원적외선이 방사되므로 품질이 향상되며, 마지막으로, 진피차 포장 공정(S7)은, 로스팅 과정을 거친, 진피차를 포장하되, 크기 0.3㎜ 이하의 진피 입자를 1 내지 1.3g의 무게로 티백에 담아 티백용으로 제공하거나, 또는, 단위량에 맞추어 포장한 다관용으로 포장하여 진피차 제조를 완료한다.This dermal particle roasting process (S6) is to heat the selected dermal particles to increase the fragrance and palatability, which is that when the heat is temporarily heated with a high temperature heat, the surface of the dermal particles burns before the inside of the dermal particles are roasted As the loss rate of falling and discarding is increased, when the halogen heat heater is used as the heat source of roasting, the inside and the outside of the dermis particles are roasted evenly to increase the taste and aroma, and the far infrared rays are radiated to improve the quality. Dermis tea packaging process (S7), the dermis, the dermal tea is packaged, but the dermis particles having a size of 0.3 mm or less packed in a tea bag with a weight of 1 to 1.3g provided for a tea bag, or packed according to the unit amount Packaged for multi-pipes to complete dermal tea production.

이와 같이 유기농 감귤을 이용한 다관용 및 티백 타입의 진피차는 온도가 높은 물, 바람직하기로는 80℃의 물에 1g 정도를 우려내어, 기호에 따라 꿀이나, 설탕 등을 첨가한 진피차를 마심으로써, 맛과 향이 우수한 진피차의 음용이 용이하고, 오래 끊이거나 달이지 않아도 되므로, 비타민 C 등의 파괴를 최소화하고, 피로회복 및 감기예방에 좋으며, 농약, 왁스와 같은 성분이 포함되지 않은 유기농 감귤을 사용하므로 인체에 해로운 화학 성분이 체내에 축적되지 않고, 보관이 용이한 특징이 있다.As described above, tea and tea bag-type dermis teas using organic citrus fruits have a high temperature, preferably 80 ° C., about 1 g, and then drink honey or sugar-derived tea. It is easy to drink dermis tea with excellent fragrance and does not have to be long or hung, so it minimizes the destruction of vitamin C, is good for fatigue and cold prevention, and uses organic tangerines that do not contain ingredients such as pesticides and waxes. There is a characteristic that chemical substances harmful to human body do not accumulate in the body and are easy to store.

한편, 제주산 온주밀감(Citrus unshiu)의 진피를 이용한 성분과 기호성 대한 실험을 하였으며, 먼저 이에 대한 실험 재료와 방법에 있어, 시료의 전처리 방법, 과피, 과육 용매 추출 및 수울 계산에 대한 내용, 총 페놀 함량 측정 방법, 디피피에이치(DPPH) 자유기 소거능 측정 방법, 세포의 독성 측정 방법, 진피차의 이화학적 검사 방법, 진피차의 관능 검사 방법 등에 대한 내용을 설명하면 다음과 같다.On the other hand, ingredients and palatability using dermis of Jeju citrus unshiu were tested. First, in the experimental materials and methods, the pretreatment method of the sample, the extract of the rind, the pulp solvent, and the calculation of the amount of water, total The phenol content measurement method, DPPH free radical scavenging ability measurement method, cell toxicity measurement method, physicochemical test method of dermis tea, sensory test method of dermis tea will be described as follows.

첫 번째, 시료의 전처리 과정을 살펴보면, 밀감을 선별 세척하고, 밀감에서 과피를 분리하며, 이 과피를 -70℃로 동결 건조 2mm이하로 분쇄한 후, 4℃에서 보관하며 사용하고, 진피차 침출 조건은 진피 건조 분말을 각각 1g씩을 취하여 실험에 사용한다.First, in the pretreatment of the sample, the mandarin is selectively washed, the peel is separated from the mandarin, and the peel is pulverized to -70 ° C. to less than 2 mm, stored at 4 ° C., and used for leaching the dermis. 1g each of the dermis dry powder is used for the experiment.

두 번째, 과피, 과육 용매 추출 및 수율 계산에 대해 살펴보면, 진피 건조 분말은 각각 핵산, 클로로포름, 에탄올 순으로 극성을 달리하면서 추출하되, 이는 진피 5g에 핵산 5㎖을 넣고, 4℃에서 30분간 항온 수조(Bio Shaker, BR-30LF, TAITEC, Japan)에서 진탕한 후, 핵산 용액을 회수하며, 그 남은 잔사에 클로로포름 5㎖을 다시 넣어 같은 방법으로 추출을 한 후 회수하고, 다시 남은 잔사에 메탄올 5㎖을 넣어 같은 방법으로 순차적으로 추출하며, 각각의 용액은 농축기(Buchi, 461, Germany)로 감압하면서 농축, 건조하여 무게를 측정하였고, 티에이치에프(THF, tetrahydrofuran) 용액 3㎖에 넣어, -20°C에 보관하면서 사용하였다.Second, the dermis, pulp solvent extraction and yield calculation, the dermal dry powder is extracted with different polarities in the order of nucleic acid, chloroform, ethanol, respectively, which is 5ml of the nucleic acid in 5g of the dermis, incubated for 30 minutes at 4 ℃ After shaking in a water bath (Bio Shaker, BR-30LF, TAITEC, Japan), the nucleic acid solution was recovered, and 5 ml of chloroform was added to the remaining residue, followed by extraction in the same manner. ㎖ was added sequentially, and each solution was concentrated under reduced pressure with a concentrator (Buchi, 461, Germany), dried and weighed. THF (THF, tetrahydrofuran) was added to 3 ml of the solution and stored at -20 ° C.

세 번째, 총 페놀 함량 측정 방법은, Folin ciocalteu 법을 일부 변형하여 측정하는데, 이는 희석된 1㎖의 시료나 표준물질(chlorogenic acid : Sigma Chemical Co., St. Louis, MO, USA)을 녹인 티에이치에프(THF) 용액 1㎖에 증류수 9㎖을 넣어 교반하고, 3분 후 Folin ciocalteu phenol reagent(Sigma Chemical Co., St. Louis, MO, USA) 1㎖을 첨가하여 교반하며, 5분 후, 7% Na2CO3 용액 10㎖을 가하여 교반하고, 증류수 25㎖로 희석한 후 23°C에서 90분 동안 정치하며, 이에 분광광도계 (DU 530 spectrophotometer, Beckman, 4300N, Fullerton, USA)를 이용하여 750 nm에서 흡광도를 측정하였고. 총 페놀함량은, chlorogenic acid (Sigma Chemical Co., St. Louis, MO, USA)로 표준 검량곡선을 작성하여 계산하였으며, 100g 습식중량에 대한 지에이이(GAE, mg gallic acid)으로 나타내었다.Third, the total phenol content measurement method is a variation of the Folin ciocalteu method, which is a tea prepared by dissolving diluted 1 ml samples or standards (chlorogenic acid: Sigma Chemical Co., St. Louis, MO, USA). 9 ml of distilled water was added to 1 ml of HF solution, followed by stirring. After 3 minutes, 1 ml of Folin ciocalteu phenol reagent (Sigma Chemical Co., St. Louis, Mo., USA) was added thereto, followed by stirring. 10 ml of 7% Na 2 CO 3 solution was added thereto, stirred, diluted with 25 ml of distilled water, and allowed to stand at 23 ° C. for 90 minutes, using a spectrophotometer (DU 530 spectrophotometer, Beckman, 4300N, Fullerton, USA). Absorbance was measured at 750 nm. The total phenol content was calculated by preparing a standard calibration curve with chlorogenic acid (Sigma Chemical Co., St. Louis, MO, USA) and expressed as mg gallic acid (GAE) for 100 g wet weight.

네 번째, 디피피에이치(DPPH, 1,1-Diphenyl-2-picrylhydrazy) 자유기 소거능 측정 방법은, Chu 등의 방법에 따라 진피 용매추출물의 농도별 희석용액 0.2㎖에, 4×10-4 M 1,1-Diphenyl-2-picrylhydrazy(DPPH : Sigma Chemical Co., St. Louis, MO, USA) 용액 0.8㎖를 가하여 10초간 혼합하고, 상온에서 10분간 방치한 후, 517nm에서 흡광도를 측정하였으며, 대조군은 티에이치에프(THF) 0.2㎖에 디피피에이치(DPPH) 용액 0.8㎖를 가한 후, 상온에서 10분간 방치한 후, 517nm에서 흡광도를 측정한 것으로 하였는데, 그 식은 다음과 같다.Fourth, the DPPH (1,1-Diphenyl-2-picrylhydrazy) free group scavenging ability measurement method, In 0.2 ml of dilution solution of the dermal solvent extract according to the method of Chu et al., 4 × 10 -4 M 1,1-Diphenyl-2-picrylhydrazy (DPPH: Sigma Chemical Co., St. Louis, MO, USA) solution 0.8 ml was added, mixed for 10 seconds, and left at room temperature for 10 minutes, and then absorbance was measured at 517 nm. The control group was added 0.2 ml of THF to 0.8 ml of DPH solution, and then added at room temperature for 10 seconds. After leaving for one minute, absorbance was measured at 517 nm. The formula is as follows.

DPPH radical scavenging activity(%) DPPH radical scavenging activity (%)

= (1-sample absorbance/control absorbance)×100  = (1-sample absorbance / control absorbance) × 100

다섯 번째, 세포 독성 측정에 대해 설명하면, PC-3, LN-CaP의 전립선암 세포는 한국 세포주 은행(KCLB)에서 분양받아 10% fetal bovine serum(FBS : Sigma Chemical Co., St. Louis, MO, USA)와 1% penicillin 및 streptomycin(Sigma Chemical Co., St. Louis, MO, USA)이 포함된 RPMI-1640 (GIBCOTM, Invitrogen, CA, USA)을 이용하여 배양하였으며, 진피 추출물의 세포독성 실험은 Nagao 등의 방법에 따라 3 - (4, 5 - dimethylthiazol - 2 -yl) -2, 5 - diphenyl tetrazolium bromide ( MTT : Sigma Chemical Co., St. Louis, MO, USA) 시약을 사용하여 실시하되, 5×104 cell/well 농도로 96 well plate에 분주하고, 37℃, 5% CO2 incubator에서 72시간 배양 한 후, 배양에 사용된 배지를 제거하며, 새로운 배지 180 μℓ와 진피 추출물 20ℓ를 첨가하되, 상기 첨가한 진피 추출물의 농도는 각각 0, 2.5, 12.5, 25.0 μg/㎖로 첨가하며, 다시 24시간 배양한 후 배지를 제거하고 0.5 mg/㎖ 농도의 엠티티(MTT)시약을 well당 200 μℓ씩 분주한 다음, 4시간 후, ELISA microplate reader(Bio-rad, Benchmark, USA)를 이용하여 595 nm에서 흡광도를 측정하였다.Fifth, let's talk about measuring cytotoxicity. Prostate cancer cells of PC-3 and LN-CaP were distributed by the Korea Cell Line Bank (KCLB) and 10% fetal bovine serum (FBS: Sigma Chemical Co., St. Louis, MO, USA), 1% penicillin and streptomycin (Sigma). Chemical Co., St. Louis, MO, USA) was cultured using RPMI-1640 (GIBCO , Invitrogen, CA, USA), and the cytotoxicity of the dermal extract was determined according to Nagao et al. 4, 5-dimethylthiazol-2 -yl) -2, 5-diphenyl tetrazolium bromide (MTT: Sigma Chemical Co., St. Louis, MO, USA) using a reagent, at a concentration of 5 × 10 4 cell / well After dispensing in a 96 well plate, incubating for 72 hours at 37 ° C., 5% CO 2 incubator, removing the medium used for the culture, and adding 180 μl of fresh medium and 20 L of dermis extract, the concentration of the added dermis extract Are added at 0, 2.5, 12.5, 25.0 μg / mL, and cultured again for 24 hours, and then the medium was removed, and 200 μL of well (MTT) reagent at a concentration of 0.5 mg / mL was used. After 4 hours, absorbance was measured at 595 nm using an ELISA microplate reader (Bio-rad, Benchmark, USA).

여섯 번째, 진피차의 이화학적 검사를 살펴보면, pH 측정은 진피 건조분말 1, 2g을 60℃, 80℃, 100℃의 물 80㎖에 각각 3분씩 침지한 후 침출액 중 5㎖를 취해 pH meter(710A, Orion, U.S.A)로, 가용성 고형분의 측정은 휴대용 굴절당도계(Atago, Japan)로 측정하여 ˚Brix로 표시하였고, 색차는 액체용 액세서리(accessary)가 부착된 색도계(Colorimeter, CM S7W, Minolta, Japan)를 사용하여 시료의 색도를 측정하되, 컬러 스페이스(Color space)는 헌터(hunter) 색체계인 L(명도), a(적색도), b(황색도)로 조정하여 측정하였다. Sixth, in the physicochemical examination of dermis tea, pH measurement was performed by immersing 1, 2 g of dried dermis powder in 80 ml of water at 60 ° C, 80 ° C, and 100 ° C for 3 minutes, and then taking 5 ml of leachate and taking a pH meter (710A). , Orion, USA), the measurement of soluble solids was measured with a hand-held refractometer (Atago, Japan) and expressed in ° Brix, and the color difference was measured by a colorimeter with a liquid accessory (Colorimeter, CM S7W, Minolta, Japan). ) Was used to measure the chromaticity of the sample, but the color space was measured by adjusting the hunter color system L (brightness), a (redness), and b (yellowness).

일곱 번째, 진피차 관능 검사 방법을 살펴보면, 이는 진피차의 관능적 차이를 알아보기 위해 서울대학교 식품영양학과 대학원 학생 20명을 대상으로 사전에 관능평가 교육, 훈련을 하고 진피 냄새, 색, 신맛, 쓴맛, 종합적 기호도를 평가였으며, 모든 특성은 7점 척도를 사용하였고, 숫자가 클수록 해당 항목의 특성이 높도록, 즉, 7점은 ‘매우 강하다’,혹은 4점은 ‘매우 좋다’, 1점은 ‘보통이다’‘매우 약하다.’ 또는, ‘매우 나쁘다.’로 표시하였다.Seventh, the dermal tea sensory test method is to examine the sensory differences between dermis tea and sensory evaluation education and training for 20 graduate students of the Department of Food and Nutrition, Seoul National University. The degree of preference was evaluated, and all the characteristics used the 7-point scale, so that the higher the number, the higher the characteristics of the item, that is, 7 points are 'very strong', or 4 points are 'very good', and 1 point is 'normal' `` Very weak, '' or `` Very bad. ''

마지막으로, 통계처리에 대해 설명하면, 이는 SAS/STAT TM User's guide 8.0판 프로그램을 이용하여 분산분석(ANOVA analysis of variance)과 Duncan's multiple range test를 이용하여 실시하고, 확률 값(Probability values)은 p<0.05수준에서 해석하였다.Finally, the statistical process is described using ANOVA analysis of variance and Duncan's multiple range test using the SAS / STAT ™ User's Guide 8.0 edition program. Probability values are p Interpretation was at the level of <0.05.

다음으로, 상기의 처리 방법을 통해 실험 결과, 즉, 진피의 일반 성분, 침출 온도별 진피차의 특성, 총 페놀 함량과 자유기 소거능, 암세포에 대한 진피 용매 추출물의 사멸효과, 진피 침출차의 관능 검사에 대한 결과를 표1 내지 표7을 참조하여 살펴보되, 첫 번째로, 진피의 일반 성분을 표1 및 표2를 참조하여 살펴보면, 먼저, 표1과 같이 가식부 100g 당 조탄수화물(61.6±0.3 mg), 조지방(15.0±20 mg), 조단백질(8.3±2.1 mg), 조회분(7.6±0.1 mg), 수분은 (7.5±0.2 mg)의 순으로 나타났으며, 다음 무기성분 함량의 분석결과 표2에서와 같이, 진피 100g당 무기질 함량은, 칼륨 3029±110 mg, 칼슘 705±20 mg, 마그네슘 495±10 mg으로 칼륨, 칼슘, 마그네슘, 인, 나트륨, 철, 아연의 순으로 나타났다. Next, the experimental results through the above treatment method, that is, the general components of dermis, characteristics of dermis tea at different leaching temperatures, total phenol content and free radical scavenging ability, killing effect of dermal solvent extracts on cancer cells, sensory test of dermis leaching tea Looking at the results with reference to Tables 1 to 7, but first, looking at the general components of the dermis with reference to Table 1 and Table 2, first, as shown in Table 1 crude carbohydrate per 100g of edible (61.6 ± 0.3 mg) , Crude fat (15.0 ± 20 mg), crude protein (8.3 ± 2.1 mg), crude ash (7.6 ± 0.1 mg), and moisture (7.5 ± 0.2 mg) in that order. As in, the mineral content per 100g of dermis was potassium 3029 ± 110 mg, calcium 705 ± 20 mg, magnesium 495 ± 10 mg in the order of potassium, calcium, magnesium, phosphorus, sodium, iron, zinc.

Physicochemical composition contents of the Citrus unshiu peel(per 100g edible portion)Physicochemical composition contents of the Citrus unshiu peel (per 100g edible portion) Moistures (g)Moistures (g) Crude protein (%)Crude protein (%) Crude fat (%)Crude fat (%) Crude carbohydrate (g)Crude carbohydrate (g) Crude ash(g)Crude ash (g) 7.5±0.27.5 ± 0.2 8.3±0.18.3 ± 0.1 15.0±0.515.0 ± 0.5 61.6±0.161.6 ± 0.1 7.6±0.17.6 ± 0.1

1)All mean values are triplicate determinations. Mean ± standard deviation. 1) All mean values are triplicate determinations. Mean ± standard deviation.

CaCa MgMg NaNa KK FeFe PP ZnZn 705±20705 ± 20 495±10495 ± 10 329±11329 ± 11 3029±1103029 ± 110 17±517 ± 5 361±35361 ± 35 7.6±0.17.6 ± 0.1

1)All mean values are triplicate determinations. Mean ± standard deviation. 1) All mean values are triplicate determinations. Mean ± standard deviation.

두 번째, 침출 온도별 진피 차의 특성을 표3을 참조하여 살펴보면, 이는 진피 침출 차 가공특성을 알아보기 위하여 침출 온도별 pH, 색도, 가용성 고형분의 양을 측정하였으며, 이는 진피 1g 차의 pH는 침출 온도 60℃에서 6.5, 100℃일 때 6.0으로 침출 온도가 높아짐에 따라 진피 차의 pH가 감소하는 것을 보이며, 또, 진피건조 분말 차 2g의 pH는 온도별로 6.3, 6.2, 5.9로 진피 1g의 온도에 따른 pH 변화와 비슷한 경향을 보였으나, 진피 2g 차가 1g 더욱더 낮은 pH값을 나타내며, 차 침출 시 pH에 영향을 주는 요소는 차중에 함유된 유기산이고, 제주산 감귤 과즙에서 검출된 유기산의 종류는 oxalic acid, citric acid, malic acid였으며, 과육에서는 citric acid가 높게 검출되었다(Song EY et al, 1998). Second, look at the characteristics of the dermis tea by leaching temperature with reference to Table 3, which measured the amount of pH, chromaticity, soluble solids by leaching temperature in order to determine the dermis leaching tea processing characteristics, which is the pH of dermis 1g tea The pH of the dermis tea decreased as the leaching temperature was increased to 6.0 at 6.5 and 100 ° C at the leaching temperature of 60 ° C. Also, the pH of the dried dermal tea 2g was 6.3, 6.2, and 5.9 for each temperature of 1g of the dermis. The pH tends to change with temperature, but 2g of dermis shows a lower pH value than 1g, and the factor affecting pH during tea leaching is the organic acid contained in tea, and the type of organic acid detected in Jeju citrus juice Were oxalic acid, citric acid and malic acid, and citric acid was detected in pulp (Song EY et al, 1998).

또한, 본 연구에도 진피 함량이 많고, 침출 온도가 높을수록 유기산의 침출이 많아져 pH가 감소하는 것으로 생각되며, 진피 침출 차의 온도별 고형물 양은 진피 1g의 경우 60, 80, 100℃에서 모두 0.9 ˚Brix이였고, 진피 2g의 경우 60, 80, 100℃에서 각각 0.9, 0.9, 1.0 ˚Brix로 나타나 침출 온도가 증가할수록 침출하는 고형분이 많아지는 것으로 보였으나 통계적으로 유의성은 없었으며, 침출 온도에 따른 침출 차의 색차는 온도가 증가할수록 명도는 낮아져 진피 1g의 차의 L값이 60℃에서 95.6 이였으나, 100℃에서 92.6을 보였다.In addition, in this study, the higher the dermis content and the higher the leaching temperature, the higher the leaching of the organic acid, which is thought to decrease the pH.The amount of solids by temperature of the dermis leaching tea was 60, 80, All were 0.9 ˚Brix at 100 ℃ and 0.9g, 0.9, 1.0 ˚Brix at 60, 80 and 100 ℃ for 2g of dermis, respectively, and the leaching solids increased with increasing leaching temperature, but there was no statistical significance. The color difference of the leaching tea according to the leaching temperature decreased with increasing temperature, and the L value of the tea of 1g of dermis was 95.6 at 60 ℃ but 92.6 at 100 ℃.

또, 진피 1g차의 적색도는 60℃에서 1.0 이였으나 100℃에서 2.8을, 황색도는 60℃에서 24.8, 100℃에서 31.5로 침출 온도가 증가할수록 적색, 황색도가 높아지는 경향을 보였고, 색차결과는 2g 진피 차도 진피 1g 차와 비슷한 색차 경향을 보였으며, 침출 분말 양이 많을수록 적색도, 황색도는 높아지는 경향을 나타내고, 따라서, 침출 온도가 증가함에 따라 침출 차의 밝기는 밝아지고, 적색도와 황색도는 증가하여 전체적으로 노란색이 강해지는 것으로 나타났다. In addition, the redness of the dermis 1g was 1.0 at 60 ° C, but 2.8 at 100 ° C and 24.8 at 60 ° C, and 31.5 at 100 ° C, respectively. The 2g dermis tea also showed a similar color difference to the 1g tea dermis, and as the amount of leaching powder increased, the redness and yellowness tended to increase. Therefore, as the leaching temperature increased, the brightness of the leaching tea became brighter and the redness and yellowness increased. The degree was increased to show that the yellow became stronger overall.

The changes of pH and color of the Citrus unshiu tea with different leaching temperatures The changes of pH and color of the Citrus unshiu tea with different leaching temperatures samples 1) samples 1) pH 2) pH 2) Soluble Solid(˚Brix)Soluble Solid (˚Brix) color 3) color 3) LL aa bb T1-60T1-60 6.5a 6.5 a 0.90.9 95.6 ± 0.4 a 95.6 ± 0.4 a 1.0 ± 0.0 c 1.0 ± 0.0 c 24.8 ± 0.4 c 24.8 ± 0.4 c T1-80T1-80 6.2ab 6.2 ab 0.90.9 93.4 ± 0.4 ab 93.4 ± 0.4 ab 1.7 ± 0.1 c 1.7 ± 0.1 c 30.9 ± 0.4 b 30.9 ± 0.4 b T-100T-100 6.0b 6.0 b 0.90.9 92.6 ± 0.3 b 92.6 ± 0.3 b 2.8 ± 0.1 a 2.8 ± 0.1 a 31.5 ± 0.3 a 31.5 ± 0.3 a T2-60T2-60 6.3a 6.3 a 0.90.9 94.1 ± 0.3 a 94.1 ± 0.3 a 2.0 ± 0.0 b 2.0 ± 0.0 b 26.7 ± 0.3 c 26.7 ± 0.3 c T2-80T2-80 6.2ab 6.2 ab 0.90.9 91.9 ± 0.5 b 91.9 ± 0.5 b 3.1 ± 0.0 a 3.1 ± 0.0 a 32.8 ± 0.2 a 32.8 ± 0.2 a T2-100T2-100 5.9b 5.9 b 1.01.0 90.0 ± 0.2 c 90.0 ± 0.2 c 3.5 ± 0.2 a 3.5 ± 0.2 a 32.9 ± 0.3 a 32.9 ± 0.3 a

1) Means 1) Means

T1-60 : One milligram of the Citrus unshiu peel tea with leaching at 60℃ for 5 minT1-60: One milligram of the Citrus unshiu peel tea with leaching at 60 ℃ for 5 min

T1-80 : One milligram of the Citrus unshiu peel tea with leaching at 80℃ for 5 minT1-80: One milligram of the Citrus unshiu peel tea with leaching at 80 ℃ for 5 min

T1-100 : One milligram of the Citrus unshiu peel tea with leaching at 100℃ for 5 minT1-100: One milligram of the Citrus unshiu peel tea with leaching at 100 ℃ for 5 min

T2-60 : Two milligram of the Citrus unshiu peel tea with leaching at 60℃ for 5 minT2-60: Two milligram of the Citrus unshiu peel tea with leaching at 60 ℃ for 5 min

T2-80 : Two milligram of the Citrus unshiu peel tea with leaching at 80℃ for 5 minT2-80: Two milligram of the Citrus unshiu peel tea with leaching at 80 ℃ for 5 min

T2-100 : Two milligram of the Citrus unshiu peel tea with leaching at 100℃ for 5 minT2-100: Two milligram of the Citrus unshiu peel tea with leaching at 100 ℃ for 5 min

1)All mean values are triplicate determinations. Mean ± standard deviation. 1) All mean values are triplicate determinations. Mean ± standard deviation.

2) and 3) Values in the same column that are followed by a different letter are significantly different(p<0.05) by Duncan's multiple range test. 2) and 3) Values in the same column that are followed by a different letter are significantly different (p <0.05) by Duncan's multiple range test.

세 번째, 총 페놀 함량과 자유기 소거능을 표4를 참조하여 살펴보면, 진피로부터 추출한 3가지 용매 추출물의 항산화 활성을 실시한 결과 진피 추출물 중 에탄올 추출물이 높은 총 페놀 함량과 자유기 소거 효과를 나타냈으나, 클로로포름 용매 추출물의 총 페놀 함량 또한 다른 감귤류와 비슷한 함량을 나타내었다.Third, looking at the total phenol content and free radical scavenging ability with reference to Table 4, the antioxidant activity of the three solvent extracts extracted from the dermis showed high total phenolic content and free radical scavenging effect. The total phenolic contents of the solvent extracts of chloroform also showed similar contents to other citrus fruits.

Recovered weight, the total phenolics contents and scavenging activity of DPPH radicals of the Citrus unshiu peel extracts Recovered weight, the total phenolics contents and scavenging activity of DPPH radicals of the Citrus unshiu peel extracts Peel extractPeel extract Weight recoverd(㎎/㎖)Weight recoverd (mg / ml) Total phenolics 2) Total phenolics 2) Scavenging activity(%) 3) Scavenging activity (%) 3) HexaneHexane 9.179.17 65.1c 65.1 c 55.3c 55.3 c ChloroformChlororoform 9.909.90 76.9b 76.9 b 65.2b 65.2 b EthanolEthanol 34.3834.38 89.0a 89.0 a 70.2a 70.2 a

1)All mean values are triplicate determinations. Values in the same column (total phenolics and scavenging activity) that are followed by a different letter are significantly different (p<0.01) by Duncan's multiple range test. 1)Total phenolics content, expressed in milligrams of gallic acid equivalents per 100g of fresh fruits. 3) Means of DPPH radical scavenging activity on 1 mg/mL of each extract 1) All mean values are triplicate determinations. Values in the same column (total phenolics and scavenging activity) that are followed by a different letter are significantly different (p <0.01) by Duncan's multiple range test. 1) Total phenolics content, expressed in milligrams of gallic acid equivalents per 100g of fresh fruits. 3) Means of DPPH radical scavenging activity on 1 mg / mL of each extract

네 번째, 암세포에 대한 진피 용매 추출물의 세포사멸 효과를 설명하면, 이는 먼저, 진피 3가지 용매 추출물의 전립선 암세포 억제 효과를 살펴보기 위하여 PC-3, LN-CaP의 전립선 암세포에 대한 세포 사멸실험을 하였고, 그 결과를 표5 및 표6에 도시하였으며, 본 실험에 사용한 PC-3, LN-CaP 세포는 모두 androgen에 의존적인데, 이 androgen 호르몬은 남자의 남성성을 대표하는 호르몬으로, 이 호르몬의 과량 분비는 전립선 암세포의 성장을 돕는(Jane M. 1998) 특징이 있고. 따라서, 2가지 세포에 추출물을 처리해서 세포 독성을 살펴봄으로써 호르몬 의존적인 과정을 효과적으로 억제하는지 알 수 있었으며, 진피 용매 추출물 중 에탄올 용매 추출물은 두 가지 암세포에 억제 효과가 모두 좋은 것으로 나타나며, 즉, 진피 에탄올 추출물 모두 PC-3, LN-CaP 세포에서 대조군에 비하여 60% 정도의 높은 억제율을 나타낸 것으로, 세포상에서 진피 용매 추출물이 전립선암을 억제한다고 할 수 있다. Fourth, explaining the apoptosis effect of the dermal solvent extract on cancer cells, first, to examine the prostatic cancer cell inhibitory effect of the three solvent extracts of the dermis, PC-3, LN-CaP cell death experiments on prostate cancer cells The results are shown in Tables 5 and 6, and the PC-3 and LN-CaP cells used in this experiment are all dependent on androgen, and this androgen hormone is a hormone representing male masculinity. Excessive secretion is characterized by the growth of prostate cancer cells (Jane M. 1998). Therefore, it was found that the treatment of the extracts on the two cells effectively inhibited the hormone-dependent process by examining the cytotoxicity. The ethanol solvent extract of the dermal solvent extracts showed a good inhibitory effect on both cancer cells, that is, the dermis. Both ethanol extracts showed a high inhibitory rate of about 60% in PC-3 and LN-CaP cells compared to the control group, and the dermal solvent extracts inhibited prostate cancer on the cells.

마지막으로, 진피 침출 차의 관능검사를 설명하되, 온도 수준을 달리하여 침출한 진피 차의 관능적 검사 결과를 나타낸 표7를 참고하여 설명하면, 이는 관능검사항목에서 진피 냄새, 색, 신맛, 쓴맛, 종합적 기호도를 평가하였고, 진피 냄새, 색깔, 종합적 기호도 항목에서 유의적 차이를 보여주며, 진피 1g의 진피냄새는 60℃에서 4.30, 80℃에서 4.92, 100℃에서 5.99로 100℃ 온도에서 침출한 진피 차의 진피 냄새가 가장 강하다고 평가 되었으며(p<0.05), 이것은 진피 2g의 경우도 같은 결과로 온도가 높을수록 진피 냄새가 강해지는 것을 알 수 있었다.Finally, the sensory test of the dermis leaching tea is described, but referring to Table 7 showing the sensory test results of the leaching tea with different temperature levels, the sensory test items include dermal odor, color, sourness, bitter taste, The overall acceptability was evaluated, and there was a significant difference in dermal odor, color and overall acceptability.The dermal odor of dermis 1g was 4.30 at 60 ℃, 4.92 at 80 ℃, 5.99 at 100 ℃, and leached at 100 ℃. The dermal odor of tea was evaluated to be the strongest (p <0.05). This is the same result for 2g of dermis. As the temperature is higher, the dermal odor becomes stronger.

그리고 상기 표3의 색차 결과에 보인 바와 같이, 침출 온도가 높을수록 진피 차의 b값(황색도)이 유의적으로 증가하는 것으로 나타났는데, 관능검사 결과 또한 진피 양이 많고, 침출 온도가 높을수록 진피 차의 색이 노랗다고 평가되었으며, 침출 온도가 증가할수록 진피 차의 신맛과 쓴맛도 점차 높게 평가되었으나 유의적 차이는 없었으며, 종합적 기호도는 진피 1g의 경우 60℃에서 3.84, 80℃에서 5.42, 100℃에서 4.11로 평가되었고, 진피 2g의 경우 각각 3.55, 4.33, 4.00으로 진피 1g을 80℃ 온도에서 침출한 진피 차의 종합적 기호도가 가장 높게 평가되었다. As shown in the color difference results of Table 3, the higher the leaching temperature, the higher the b-value (yellowness) of the dermis difference was observed. The color of the dermis tea was evaluated as yellow, and as the leaching temperature increased, the sour and bitter taste of the dermis tea were gradually evaluated, but there was no significant difference.The overall acceptability was 3.84 at 60 ℃ and 5.42 at 80 ℃ for 1g of dermis. In the case of 2g of dermis, the overall acceptability of the dermis tea, which leached 1g of dermis at 80 ° C, was evaluated to be 4.11 at 100 ° C.

따라서, 이것은 100℃와 같이 고온에서 진피 차를 침출하여 음용 시 진피의 여러 가지 향기와 떫은맛 성분들이 함께 우려 나와 80℃에서 침출한 진피 차의 기호도보다 낮게 평가되는 것으로 사려되며, 반대로 60℃와 같은 온도에서는 진피의 독특한 성분들이 충분히 침출되지 않아 기호도가 낮게 평가되는 것으로 보이므로, 진피 1g 양을 80℃에서 침출하여 진피 차를 음용 하는 것이, 진피 차의 기호도를 높일 수 있는 바람직한 방법으로 사료된다. Therefore, this is considered to be lower than the preference of the dermis tea leached at 80 ℃ because the various flavors and astringent components of the dermis are leached at the high temperature such as 100 ℃ to drink the drink Since the unique components of the dermis are not sufficiently leached at the temperature, and thus the palatability seems to be low, it is considered that leaching the dermis tea by leaching the amount of 1 g of the dermis at 80 ° C. is considered a preferable method to increase the palatability of the dermis tea.

Scores by sensory evaluation of the Citrus unshiu tea with different leaching temperaturesScores by sensory evaluation of the Citrus unshiu tea with different leaching temperatures samples 1) samples 1) Sensory evaluation 2) Sensory evaluation 2) Citrus aromaCitrus aroma ColorColor SourSour BitterBitter Overall acceptabilityOverall acceptability T1-60T1-60 4.30c 4.30 c 3.85b 3.85 b 3.903.90 4.784.78 3.84b 3.84 b T1-80T1-80 4.92ab 4.92 ab 4.94ab 4.94 ab 4.004.00 4.764.76 5.42a 5.42 a T-100T-100 5.99a 5.99 a 5.24a 5.24 a 4.084.08 4.794.79 4.11b 4.11 b T2-60T2-60 4.80c 4.80 c 3.99b 3.99 b 3.923.92 4.774.77 3.55c 3.55 c T2-80T2-80 5.92a 5.92 a 5.02ab 5.02 ab 4.044.04 4.884.88 4.33b 4.33 b T2-100T2-100 6.09a 6.09 a 5.65a 5.65 a 4.094.09 4.924.92 4.00b 4.00 b

1) Means 1) Means

T1-60 : One milligram of the Citrus unshiu peel tea with leaching at 60℃ for 5 minT1-60: One milligram of the Citrus unshiu peel tea with leaching at 60 ℃ for 5 min

T1-80 : One milligram of the Citrus unshiu peel tea with leaching at 80℃ for 5 minT1-80: One milligram of the Citrus unshiu peel tea with leaching at 80 ℃ for 5 min

T1-100 : One milligram of the Citrus unshiu peel tea with leaching at 100℃ for 5 minT1-100: One milligram of the Citrus unshiu peel tea with leaching at 100 ℃ for 5 min

T2-60 : Two milligram of the Citrus unshiu peel tea with leaching at 60℃ for 5 minT2-60: Two milligram of the Citrus unshiu peel tea with leaching at 60 ℃ for 5 min

T2-80 : Two milligram of the Citrus unshiu peel tea with leaching at 80℃ for 5 minT2-80: Two milligram of the Citrus unshiu peel tea with leaching at 80 ℃ for 5 min

T2-100 : Two milligram of the Citrus unshiu peel tea with leaching at 100℃ for 5 minT2-100: Two milligram of the Citrus unshiu peel tea with leaching at 100 ℃ for 5 min

1)All mean values are triplicate determinations. Mean ± standard deviation. 1) All mean values are triplicate determinations. Mean ± standard deviation.

2) and 3) Values in the same column that are followed by a different letter are significantly different(p < 0.05) by Duncan's multiple range test. 2) and 3) Values in the same column that are followed by a different letter are significantly different (p <0.05) by Duncan's multiple range test.

이와 같이 본 발명은, 유기농 감귤의 과피를 반건조한 진피를 일정 크기 이하로 분쇄하고, 이를 그 크기에 따라 다관용 및 티백용으로 분류하며, 이 분류된 진피 입자에 열을 가해 풍미를 더하는 로스팅 공정과, 적절한 포장을 거쳐 진피차를 제조하여, 이를 온도가 높은 물에 우려내어 맛과 향이 우수한 진피차를 용이하게 마실 수 있음에 따라, 비타민 C의 파괴를 최소화하고, 피로회복과 감기예방, 피부미용에 좋으며, 보관이 용이한 효과가 있다.As described above, the present invention is to crush the semi-dried dermis of the organic citrus peel to a certain size or less, and classify it as a multi-use and tea bag according to its size, and add a flavor by applying heat to the classified dermis particles It is possible to prepare dermis tea through proper packaging, so that it can be easily drunk with high temperature and high taste and flavor, so that the destruction of vitamin C can be minimized, and fatigue recovery, cold prevention, and skin care are possible. It is good and has the effect of easy storage.

도 1은 본 발명에 따른 공정도.1 is a process chart according to the present invention.

Claims (3)

유기농 감귤을 씻어 물기를 없애는 감귤의 세척 및 건조 공정(S1), 상기 감귤의 껍질에서 과육 부분을 분리하여 과피를 모으는 감귤의 과육 및 과피 분리 공정(S2), 분리된 과피를 반건조하는 과피 건조 공정(S3), 건조된 과피인 진피를 크기 0.8㎜이하로 잘게 분쇄하는 진피 분쇄 공정(S4), 분쇄된 진피 입자를 그 크기에 따라 0.5 내지 0.8㎜의 다관용과, 그 이하의 티백용으로 나뉘는 진피 입자 분류 공정(S5), 분류된 진피 입자를 각각 열가공하는 진피 입자 로스팅 공정(S6), 마지막으로, 상기 로스팅한 진피 입자를 분류된 대로 다관용과 티백용으로 각각 포장하는 진피차 포장 공장(S7)으로 진피차를 제조함을 특징으로 하는 유기농 감귤을 이용한 진피차의 제조 방법.Citrus washing and drying process of washing organic tangerines to remove moisture (S1), peeling fruit and peeling process of citrus fruit (S2) to separate the flesh part from the peel of the tangerine, and drying the peeled skin to semi-dried separated skin Process (S3), the dermis grinding step (S4) to finely pulverize the dried dermis to a size of 0.8 mm or less, the pulverized dermal particles for 0.5 to 0.8 mm of multi-tubules, and tea bags below The dermis particle sorting process (S5) which is divided into dermis particle roasting process (S6) which heat-processes the sorted dermis particles, respectively, and finally, the dermis tea packing plant which packs the roasted dermis particles separately for assortment for tea and tea bags. (S7) Method of producing dermis tea using organic tangerines, characterized in that the dermis tea is produced. 제 1항에 있어서,The method of claim 1, 상기 진피차 포장 공장(S7)에서는, 크기 0.3㎜ 이하의 진피 입자를 1 내지 1.3g로 티백에 담아 티백용으로 포장됨을 특징으로 하는 유기농 감귤을 이용한 진피차의 제조 방법. In the dermis tea packaging plant (S7), the dermis particles of less than 0.3 mm in size in a tea bag packed in a tea bag 1 to 1.3 g, the method for producing dermis tea using organic tangerines, characterized in that for packaging. 제 1항 또는 제 2 항에 있어서,The method according to claim 1 or 2, 상기 진피 입자 로스팅 공정(S6)에서는, 할로겐 라이트 히터를 이용한 열가공을 함을 특징으로 하는 유기농 감귤을 이용한 진피차의 제조 방법. In the dermal particle roasting step (S6), a method of producing dermis using organic tangerines, characterized in that the heat processing using a halogen light heater.
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KR101226459B1 (en) * 2010-12-08 2013-01-28 문근식 Manufacturing Method of Dried Citrus Unshiu Peel Tea And Citrus Unshiu Peel Tea Using The Same
KR101326076B1 (en) * 2013-07-19 2013-11-07 이종민 Leaching type citron tea and manufacturing method thereof and tea bag contained leaching type citron tea
KR20160011461A (en) * 2014-07-22 2016-02-01 롯데칠성음료주식회사 Mixed tea composition of herbal medicine having the improved taste, and preparation thereof
KR20220132872A (en) * 2021-03-24 2022-10-04 더불어 주식회사 Manufacturing method of citrus peel tea
WO2023121300A1 (en) * 2021-12-21 2023-06-29 주식회사 뉴트라잇 Composition comprising extract of roasted citrus by-products as active ingredient for prevention, amelioration, or treatment of sleep disorder or insomnia

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KR100840639B1 (en) 2007-01-29 2008-06-24 동우당제약(주) Tea which tangerine peel is used as main materials and its manufacturing method
KR101268845B1 (en) 2011-11-07 2013-05-29 허담 Garnish of solid tea with tangerine peel and its manufacturing method

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101226459B1 (en) * 2010-12-08 2013-01-28 문근식 Manufacturing Method of Dried Citrus Unshiu Peel Tea And Citrus Unshiu Peel Tea Using The Same
KR101326076B1 (en) * 2013-07-19 2013-11-07 이종민 Leaching type citron tea and manufacturing method thereof and tea bag contained leaching type citron tea
KR20160011461A (en) * 2014-07-22 2016-02-01 롯데칠성음료주식회사 Mixed tea composition of herbal medicine having the improved taste, and preparation thereof
KR20220132872A (en) * 2021-03-24 2022-10-04 더불어 주식회사 Manufacturing method of citrus peel tea
WO2023121300A1 (en) * 2021-12-21 2023-06-29 주식회사 뉴트라잇 Composition comprising extract of roasted citrus by-products as active ingredient for prevention, amelioration, or treatment of sleep disorder or insomnia

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