KR20040082061A - a soy sediment laws governing manufacture - Google Patents

a soy sediment laws governing manufacture Download PDF

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KR20040082061A
KR20040082061A KR1020030016670A KR20030016670A KR20040082061A KR 20040082061 A KR20040082061 A KR 20040082061A KR 1020030016670 A KR1020030016670 A KR 1020030016670A KR 20030016670 A KR20030016670 A KR 20030016670A KR 20040082061 A KR20040082061 A KR 20040082061A
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beans
soybeans
red pepper
water
fermented
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KR100496568B1 (en
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송태영
김세환
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송태영
김세환
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/50Fermented pulses or legumes; Fermentation of pulses or legumes based on the addition of microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L11/00Pulses, i.e. fruits of leguminous plants, for production of food; Products from legumes; Preparation or treatment thereof
    • A23L11/05Mashed or comminuted pulses or legumes; Products made therefrom
    • A23L11/07Soya beans, e.g. oil-extracted soya bean flakes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/09Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/10Natural spices, flavouring agents or condiments; Extracts thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/015Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with pressure variation, shock, acceleration or shear stress or cavitation
    • A23L3/0155Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with pressure variation, shock, acceleration or shear stress or cavitation using sub- or super-atmospheric pressures, or pressure variations transmitted by a liquid or gas
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/16Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by heating loose unpacked materials
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L31/00Edible extracts or preparations of fungi; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23PSHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00Shaping or working of foodstuffs characterised by the products
    • A23P10/40Shaping or working of foodstuffs characterised by the products free-flowing powder or instant powder, i.e. powder which is reconstituted rapidly when liquid is added
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • A23V2250/208Fungi extracts

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Agronomy & Crop Science (AREA)
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  • Biotechnology (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

PURPOSE: A method of making fermented soybean paste and red pepper paste by culturing the mycelium of Phellinus linteus in steamed beans is provided. The fermented soybean paste and red pepper paste have synergistic action between pharmacological efficacy of Phellinus linteus and anticancer effect of the fermented food and thus are effective for prevention of cancer. CONSTITUTION: Beans are soaked in warm water of 80deg.C for 2hr, steamed for 20min and then added with calcium carbonate to control the pH to 6.5. The beans are sterilized for 1.5hr in a pressurized tank maintaining the temperature of 130deg.C and the pressure of 1.5atm, inoculated with the mycelium of Phellinus linteus and then cultured at 22 to 25deg.C for 15 days. They are aged for 1 to 3 months to give fermented soybean paste. The fermented beans prior to aging are dried to a moisture content to 10% or less, ground and then mixed with starch powder, red pepper powder, starch sugar and salt. Then the mixture is aged for 3 months or more to give red pepper paste.

Description

상황버섯 균사체를 이용한 된장 및 고추장의 제조방법{a soy sediment laws governing manufacture}Manufacturing method of Doenjang and Gochujang using Situation Mushroom Mycelia {a soy sediment laws governing manufacture}

본 발명은 상황버섯 균사체를 이용한 된장 및 고추장의 제조방법에 관한 것으로서, 더욱 상세하게는 상황버섯의 균사체를 증자된 콩에 배양하여 항암 효과가 매우 뛰어난 된장 및 고추장을 제공함에 있는 것이다.The present invention relates to a method for producing doenjang and red pepper paste using the situation mushroom mycelium, and more particularly, to provide a doenjang and red pepper paste having excellent anticancer effect by cultivating the mycelia of the situation mushroom in the soybean.

일반적으로 상황버섯은 민주름 버섯목 구멍장이 버섯과에 속하는 목재부후성 담자균류에 속하며 예로부터 위통, 무릎 관절통 등에 우수한 효능을 발휘하는 한방약재로 널리 알려져 있고, 근래에는 상황버섯의 균사체를 액체 배양한 결과 폴리사카라이드계의 항암 면역 활성물질을 생산하는 것이 발견되었다.In general, the situation mushroom is a woody mildew fungus belonging to the Democratic Mushroom Fungus, which belongs to the mushroom family, and has been widely known as an herbal medicine that shows excellent efficacy in stomach pain and knee joint pain, etc. As a result, it has been found to produce anti-cancer immunoactive substances of polysaccharides.

전술한 항암 면역 활성물질은 면역기능을 강화하는 인터루킨이나 인터페론등 사이토킨의 생산을 촉진하여 세포면역과 체액면역에 의한 면역기능을 현저히 보강시켜 각종 종양과 바이러스 감염에 대한 저항력을 높일 수 있는 것으로서 항암 효과가 매우 뛰어난 것으로 밝혀진 바 있다.The above anti-cancer immune active substance promotes the production of cytokines such as interleukin or interferon to enhance immune function, thereby reinforcing the immune function by cellular immunity and humoral immunity, thereby increasing resistance to various tumors and viral infections. Has been found to be very good.

그러나, 전술한 상황버섯은 자연계에서는 널리 분포되지 않은 휘귀종이기 때문에 수량이 많지 않아 가격이 비싸고 일반인이 쉽게 구하기 어려워 약재 이외의 용도로 사용되는 경우가 거의 없는 실정이지만, 근래에는 상황버섯의 균사체를 인공적으로 배양하여 재배하는 방법이 개발되어 다양하게 활용되고 있다.However, the above-mentioned situation mushroom is a rare species that is not widely distributed in the natural world, so the quantity is not high and the price is high and it is difficult to obtain easily by the general public. Artificially cultured and cultivated methods have been developed and utilized in various ways.

그러나, 상황버섯의 인공재배는 기술적으로 매우 까다로워 기술적인 문제가 제기되고, 여름에만 생산이 가능한 것이므로 생산되는 계절이 제한되어 소비자들에게 충분한 양의 상황버섯을 제공할 수 없었다.However, artificial cultivation of situation mushrooms is technically very difficult, and technical problems have been raised, and production only in summer, so that the seasons produced are limited, so it is not possible to provide a sufficient amount of situation mushrooms to consumers.

따라서, 상황버섯의 가격이 여전히 비싸게 형성되는 관계로 소비자들에게 저렴한 가격대의 상황버섯을 충분히 제공할 수 없었으므로 상황버섯의 사용범위가 상당히 제한적이었다.Therefore, since the price of the situation mushroom is still expensive, the range of use of the situation mushroom was quite limited because it was not possible to provide consumers with the situation mushroom at a low price.

한편, 된장이나 고추장과 같은 장류는 예로부터 널리 이용된 우리의 전통 발효식품으로서 영양분이 풍부하고 암의 발생을 억제하는 효과가 뛰어난 식품이다.On the other hand, jangs such as doenjang and red pepper paste are our traditional fermented foods that have been widely used since ancient times, and are rich in nutrients and excellent in inhibiting cancer.

또한, 된장이나 고추장을 담그는 주원료인 콩(大豆)에는 식물성 피토에스트로겐인 이소플라본이 함유되어 있고, 이소플라본은 각종 암을 억제하고 골다공증, 심혈관 질환, 갱년기 증상을 예방할 수 있을 뿐 아니라 다양한 생리활성 작용을 나타내는 제니스틴, 데이드진, 글라이시틴으로 이루어진 단백질 화합물로 보고되고 있다.In addition, soybean (大豆), the main ingredient for soybean paste or red pepper paste, contains isoflavone, a phytoestrogen, and isoflavone can suppress various cancers and prevent osteoporosis, cardiovascular disease, menopausal symptoms, and various physiological activities. It has been reported as a protein compound consisting of Genistin, Dadezine, and Glycithin.

이와 같은 이소플라본의 효과는 카톨릭의대에서 열린 국제 암예방학회 학술대회와 제3회 국제암심포지엄에서 밝혀진 바 있고, 이 학술대회에서 미국 알라마바대학 약리독성학과 스테픈 반슨 교수는 "생후 2∼20일된 쥐를 대상으로 발암물질을 투여한 후 다시 이소플라본을 투여한 결과 종양이 50%이상 억제되었다"고 밝혔고 이외에도 여러 학술논문에서 이소플라본의 항암효과를 보고하고 있다.This effect of isoflavones was revealed at the International Cancer Prevention Conference and the 3rd International Cancer Symposium held at the Catholic Medical School.In this conference, Professor Stephen Barnson, Department of Pharmacology and Toxicology, University of Alabama, Isoflavones were suppressed by over 50% after administration of carcinogens to Japanese rats. ”In addition, several academic papers report the anticancer effects of isoflavones.

따라서, 상황버섯의 균사체를 이용하여 콩을 직접 발효 숙성시켜 된장이나 고추장을 제조하면 각각의 재료들이 갖는 효능들의 상충작용에 의하여 항암 효과를 극대화시킬 수 있는 완전한 식품의 제조가 가능하다는 것을 발견하였고, 이에 균사체를 이용한 된장 및 고추장을 개발하게 되었다.Therefore, it was found that the preparation of soybean paste or red pepper paste by fermenting soybeans directly using the mycelium of situation mushrooms enables the production of complete foods that can maximize the anticancer effect by the conflicting effects of the ingredients. Soybean paste and red pepper paste using mycelium were developed.

본 발명은 상기한 문제점을 감안하여 창안한 것으로서, 그 목적은 상황버섯의 균사체를 증자된 콩에 배양하여 발효 및 숙성시켜 항암 효과가 매우 뛰어난 된장 및 고추장을 제공함에 있는 것이다.The present invention has been made in view of the above problems, and its object is to provide a doenjang and red pepper paste with excellent anticancer effect by culturing the mycelium of the situation mushrooms in the soybean and fermenting and aging.

상기한 목적을 달성하기 위한 본 발명의 특징은, 품종이 우수한 양질의 콩을 선별하여 정화된 깨끗한 물로 세척하는 단계; 세척된 콩을 80℃의 온수에 담그고, 2시간 동안 침지시켜 콩을 불리는 단계; 불려진 콩을 탱크에 투입하여 함께 주입된 물을 끓이거나 고열의 스팀을 부여하여 20분간 삶아서 콩을 팽화(膨化)시키는 증자단계; 삶아진 콩에 물기를 제거한 후 탄산칼슘을 고르게 투여하여 콩의 수분함유량이 50%를 유지되도록 하고, 수소이온농도(pH)가 6.5정도를 유지토록 하여약산성으로 조절하는 콩의 수분 및 산도의 조절단계; 수분 및 산도가 적절하게 조절된 콩을 1.5기압과 130℃를 유지하는 압력탱크 내부에 투입하여 1시간 30분간 압력과 열을 가하여 각종 유해세균들을 멸균시키는 단계; 멸균된 콩을 식혀서 냉각시키는 단계; 증자되어 팽화된 콩을 접종실에 투입하여 액상의 상황버섯 균사체를 고르게 분무하여 종균을 접종하는 단계; 종균이 접종된 콩을 배양실의 온도가 22∼25℃를 유지한 상태에서 15일간 배양하여 콩을 발효시키는 단계; 발효된 콩을 소금물에 담그어 1∼3개월 숙성(aging)시키는 단계에 의하여 된장이 완성됨을 특징으로 하는 상황버섯 균사체를 이용한 된장의 제조방법에 의하여 달성될 수 있는 것이다.Features of the present invention for achieving the above object, the step of selecting a variety of high-quality beans and washing with purified clean water; Soaking the washed beans in warm water at 80 ℃, soaking for 2 hours to call the beans; A steaming step in which the soaked beans are put into a tank to boil the water injected together or given high heat steam to boil for 20 minutes to swell the beans; Water is removed from the boiled beans and calcium carbonate is evenly administered to maintain the water content of the soybeans at 50% and to control the moisture and acidity of the soybeans to be slightly acidic to maintain a pH of 6.5. step; Sterilizing various harmful bacteria by applying pressure and heat for 1 hour and 30 minutes in a pressure tank maintained at 1.5 atm and 130 ° C., so that the moisture and acidity are properly adjusted; Cooling the sterile beans by cooling; Injecting the increased and expanded bean into the inoculation chamber to inoculate the spawn by spraying evenly the liquid situation mushroom mycelium; Fermenting the soybeans inoculated with the spawn seed incubated for 15 days while maintaining the temperature of the culture chamber at 22 to 25 ° C; The fermented soybeans can be achieved by the method of producing miso using the situation mushroom mycelium, characterized in that the miso is completed by immersing in fermented salt water for 1 to 3 months.

도 1은 본 발명의 제조과정을 순차적으로 나타낸 제조 공정도.1 is a manufacturing process diagram sequentially showing the manufacturing process of the present invention.

이하, 상기한 목적을 달성하기 위한 바람직한 실시예를 도 1에서 도시한 제조 공정도를 참조하여 상세히 설명하면 다음과 같다.Hereinafter, a preferred embodiment for achieving the above object will be described in detail with reference to the manufacturing process diagram shown in FIG.

품종이 우수한 양질의 콩(大豆)을 선별하여 정화된 깨끗한 물로 세척하고, 세척된 콩을 약 80℃를 유지하는 온수(溫水)에 담근 후 약 2시간 동안 침지시켜 콩을 불린다.High-quality soybeans (大豆) with excellent varieties are selected and washed with purified water, soaked in soaked water for about 2 hours and soaked in soybeans.

이 단계에서는 선별된 양질의 콩을 깨끗한 물로 세척하여 콩에 달라붙은 이물질들을 제거하는 역할과 함께 콩을 물에 침지(沈漬)시켜 충분한 수분이 콩에 흡수되도록 하여 콩이 불려지도록 하여 이어지는 증자단계가 원활하게 이루어지도록 하는 역할을 수행한다.In this step, the selected high quality soybeans are washed with clean water to remove foreign substances stuck to the soybeans, and the soybeans are soaked by soaking enough water to soak in the soybeans. It plays a role to make the operation smoothly.

이어서, 충분히 불려진 콩을 물과 함께 탱크에 투입하여 물을 끓이거나 고열의 스팀을 부여하여 약 20분간 삶아서 콩을 팽화(膨化)시킨다.Subsequently, enough soy beans are poured into the tank together with water to boil the water or boil for about 20 minutes by applying high heat steam to swell the beans.

이 단계는 불려진 콩을 삶거나 고열의 스팀을 부여하여 콩을 증자시키는 단계로 콩이 팽화되어 분자간의 간격이 넓어지면서 겔(gel) 상태를 유지함에 따라 종균의 배양이 원활하게 이루어질 수 있도록 하는 중요한 단계이다.This step is to boil the soybeans or to give steam at high temperature to increase the soybeans. The soybean is expanded and the gap between molecules widens to maintain the gel state. Step.

이어서, 삶아진 콩에 물기를 제거한 후 탄산칼슘을 고르게 투여하여 콩의 수분함유량이 50%를 유지하도록 하고, 수소이온농도(pH)는 6.5를 유지하도록 하여 약산성(弱酸性)으로 조절한다.Subsequently, after the water is removed from the boiled soybeans, calcium carbonate is evenly administered to maintain the water content of the soybeans at 50%, and the hydrogen ion concentration (pH) is maintained at 6.5 so as to be slightly acidic.

이 단계는 콩의 수분 함유량과 산도를 적절하게 조절하여 종균이 배양되기 위한 최적의 조건을 제공하는 중요한 단계이다.This step is an important step to provide the optimum conditions for the spawn to be grown by properly adjusting the water content and acidity of the beans.

이때 사용되는 탄산칼슘(CaCO3)은 자연계에 존재하는 염 중에서 가장 많고 그 형태도 여러 가지이나 주로 조개껍질을 습식 분쇄하고 멸균처리 한 호분(胡粉)을 사용하는 것이 바람직하다.At this time, calcium carbonate (CaCO3) is the most number of salts present in nature and many forms, but it is preferable to use a wet crushed and sterilized crushed clam shell.

조개껍질을 추출한 탄산칼슘은 식용으로 사용할 수 있는 것이므로 인체에 무해하면서도 값이 싸고, 비중도 크지 않아 구입 및 사용이 편리할 뿐 아니라 수분을 흡착하므로 수분함유량의 조절이 용이하면서도 산성을 중화시켜 산도를 적절하게 조절하는 역할을 수행한다.Calcium carbonate extracted from shellfish is harmless to human body because it can be used for food. It is not easy to purchase and use because it is not expensive. It is easy to purchase and use, and it absorbs moisture, so it is easy to control moisture content and neutralize acidity. It has the role of making appropriate adjustments

이어서, 수분 및 산도가 적절하게 조절된 콩을 1.5기압과 130℃를 유지하는 압력탱크 내부에 투입하여 약 1시간 30분 정도 멸균시켜 각종 유해세균들을 제거한다.Subsequently, the soybeans in which water and acidity are properly adjusted are put into a pressure tank maintaining 1.5 atm and 130 ° C. to sterilize for about 1 hour and 30 minutes to remove various harmful bacteria.

이 단계에서는 콩의 발효에 나쁜 영향을 미치는 각종 유해세균을 제거하여 멸균시키는 단계로 콩의 발효를 활성화시키는 중요한 역할을 한다.In this step, it is important to activate the fermentation of soybeans by sterilizing them by removing various harmful bacteria which adversely affect soybean fermentation.

이어서, 멸균된 콩을 식혀서 30∼40℃로 냉각시킨 후 팽화된 콩을 접종실에 투입하여 액상의 상황버섯 균사체를 고르게 분무하여 종균을 접종한다.Subsequently, the sterilized soybeans are cooled and cooled to 30 to 40 ° C., and then the swollen soybeans are added to the inoculation chamber to inoculate the seedlings by evenly spraying the liquid situation mushroom mycelia.

상기 상황버섯 균사체는 상황버섯 배양지에서 배양시킨 액체 균사체를 구입하여 사용하였다. 그러나 균사체는 통상적으로 사용되는 방법으로 배양하여 콩에 접종할 수 있다.The situation mushroom mycelium was used to purchase a liquid mycelium cultured in a situation mushroom culture. However, mycelium can be inoculated in soybean by culturing in a conventional manner.

예를 들면 상황버섯의 원균을 액체배지에서 20∼35℃의 온도로 배양하는 방법으로 상황버섯 종균배양을 완료한 후, 액체 배지를 용기에 주입하여 20∼25℃의 온도에서 약한 빛을 조사(照射)하면서 17∼30일간 배양하는 방법으로 상황버섯의 균사체를 형성하고, 찌꺼기를 여과시킨 후 균사체와 물을 4 : 6 비율로 혼합하여 희석시킨 균사체 용액을 증자된 콩에 고르게 분무하여 종균을 접종시킬 수 있었다.For example, after completion of the culture of spawn mushroom spawn by incubating the fungi of the situation mushroom in a liquid medium at a temperature of 20 to 35 ° C, the liquid medium is injected into a container and irradiated with weak light at a temperature of 20 to 25 ° C. Cultivate mycelium of situation mushrooms by culturing for 17-30 days, filter the residue, mix the mycelium with water in a ratio of 4: 6, and spray the diluted mycelium solution evenly on the soybean to inoculate spawn seed. I could make it.

이어서, 상황버섯 균사체의 종균이 완료되면 배양실의 온도가 22∼25℃를 유지되도록 한 상태에서 약 15일간 배양하여 콩을 발효시킨다.Subsequently, when the seedling of the situation mushroom mycelium is completed, the soybean is fermented by culturing for about 15 days while maintaining the temperature of the culture chamber at 22 to 25 ° C.

이 단계에서는 고열에 의하여 증자되면서 팽화된 콩을 30∼40℃로 냉각시킨 후 상황버섯의 균사체를 접종시켜 약 15일간 배양하는 단계로 균사체의 포자가 발효되면서 활성화되어 종국(種麴)이 배양된다.In this step, the soybeans expanded by high heat are cooled to 30 to 40 ° C, inoculated with mycelium of situation mushrooms, and incubated for about 15 days. .

이 단계는 팽화된 콩의 전분질 원료에서 상황버섯 균사체의 종균이 배양되어 순수한 국균을 많이 증식하여 종국을 형성하고 국균이 무수하게 증식되어 발효가 촉진되는 과정으로 후술되는 된장이나 고추장의 숙성단계를 활성화시켜 맛있고 영양분이 풍부한 장류를 제조할 수 있도록 하는 중요한 단계이다.This step is a process in which the spawn of the situation mushroom mycelium is cultured in the starch raw material of the expanded soybeans to multiply a lot of pure Korean bacteria to form a final state, and the bacteria are multiplied so that fermentation is promoted. This is an important step in making delicious and nutritious soy sauce.

이어서, 발효된 콩을 소금물에 담그고, 1∼3개월 숙성(aging)시켜 된장을 완성하였다.Then, the fermented soybeans were soaked in brine and aged for 1 to 3 months to complete the miso.

이 단계에서는 발효된 콩을 염도 17∼20%의 소금물에 침지시켜 염분 함유량이 7∼11%이 유지되도록 하고, 1∼3개월 간 숙성시키는 담금 및 숙성단계로 콩에 배양된 상황버섯 균사체의 종균이 배양된 코지에 의하여 발효 숙성되면서 된장이 완성되는 최종 단계이다.In this step, the fermented soybeans are immersed in salt water of 17 to 20% of salt so that the salt content is maintained to 7 to 11%. Fermentation and fermentation by this cultured koji is the final stage of making doenjang.

이와 같이 된장이 발효 숙성되면 콩의 주성분인 단백질과 글리코겐이 분해되면서 맛있고 영양이 풍부한 고품질의 된장을 얻을 수 있는 것이다. 이때, 상황버섯 균사체의 성분이 된장에 고르게 흡수되어 발효 및 숙성되면서 항암성이 탁월한 된장이 완성된다.When fermented and fermented soybean paste, proteins and glycogen, which are the main ingredients of soybeans, are broken down to obtain high quality miso that is delicious and nutritious. At this time, the components of the situation mushroom mycelium is evenly absorbed in the doenjang fermented and matured to complete the doenjang excellent anticancer properties.

한편, 경우에 따라서는 배양단계를 통해 발효된 콩을 소금물에 담그지 않고 콩을 건조시킨 후 분쇄하여 녹말가루, 고춧가루, 물엿, 소금을 물에 버무린 후 숙성시켜 고추장을 제조할 수도 있다.Meanwhile, in some cases, the soybean fermented through the culturing step may be dried after crushing the beans without immersion in salt water to prepare red pepper paste by soaking starch powder, red pepper powder, starch syrup, and salt in water.

즉, 배양단계에 의하여 발효된 콩을 건조실에 투입하여 수분함유량 10%이하로 건조시키는 단계; 건조된 콩을 분쇄기에 투입하여 고운 분말로 분쇄하는 단계; 분쇄된 콩 분말에 녹말가루, 고춧가루, 물엿, 소금을 고르게 혼합하여 물과 함께 버무리는 혼합단계; 버무려진 반죽물을 3개월 이상 숙성시키는 단계에 의하여 발효 숙성된 고추장을 완성할 수 있는 것이다.That is, adding the fermented soybeans to the drying chamber by the culture step to dry to less than 10% moisture content; Pulverizing the dried beans into a fine powder; A mixing step of mixing together with water by mixing starch powder, red pepper powder, starch syrup and salt with crushed soybean powder; By fermenting the soaked dough for three months or more will be able to complete the fermented aged gochujang.

상기 녹말은 찹쌀가루, 멥쌀가루, 보릿가루, 밀가루 등을 사용할 수 있고,바람직하기로는 엿기름가루를 물에 담가 아밀로오스 효소를 추출하여 그 물로 녹말을 반죽한 후 약 60℃ 이하의 온도에서 녹말의 일부를 당화시킨 다음, 녹말을 완전히 효소화시키고, 거기에 발효된 콩가루, 고춧가루, 소금, 물엿을 넣어 버무리는 방법이 바람직하며 이 방법으로 담근 고추장은 윤택이 나고 단맛이 강해 풍미가 좋은 고추장을 제공할 수 있다.The starch may be used for glutinous rice flour, non-glutinous rice flour, barley flour, flour, etc. Preferably, the malt powder is soaked in water, the amylose enzyme is extracted, the starch is kneaded with the water, and the starch is part of the starch at a temperature of about 60 ° C. or lower. After saccharifying, the starch is completely enzymatically mixed with fermented soybean powder, red pepper powder, salt, and starch syrup. The red pepper paste soaked in this way is glossy and sweet, so that it can provide good flavor. Can be.

경우에 따라서는 상기 재료에 납두균(納豆菌)을 첨가하여 60∼65 ℃에서 추가 숙성시키는 속양법(速釀法)으로 2∼3일 뒤에는 먹을 수 있도록 고추장을 제조할 수도 있다.In some cases, kochujang may be prepared so that it can be eaten after 2-3 days by adding soybean fungus to the material and further aging at 60 to 65 ° C.

전술한 방법으로 제조된 본 발명의 고추장은 녹말이 가수분해되어 생성된 당의 단맛, 콩의 가수분해로 생성된 아미노산(酸)의 구수한 맛, 고춧가루의 매운맛, 소금의 짠맛이 잘 조화되어 고추장 특유의 맛을 얻을 수 있고, 당화력과 단백질의 분해력이 강한 상황버섯 균사체를 국균(麴菌)으로 발효시킨 것이므로 상황버섯 균사체의 성분이 고르게 흡수되면서 발효 및 숙성되어 항암성이 탁월한 고추장이 완성되었다.The kochujang of the present invention prepared by the method described above is unique in that the sweet taste of sugar produced by hydrolysis of starch, the sweet taste of amino acids produced by hydrolysis of soybean, the spicy taste of red pepper powder, and the salty taste of salt are in harmony. The taste is obtained, and the state mushroom mycelium, which has strong glycosylation and protein decomposition ability, is fermented with Korean bacteria, so that the components of the situation mushroom mycelium are absorbed evenly and fermented and matured.

경우에 따라서는 전술한 제조방법에 의하여 제조된 된장과 고추장을 건조실에서 각각 건조시키고, 고운 분말로 분쇄하여 포장한 후 유통시킬 수도 있다.In some cases, the doenjang and red pepper paste prepared by the above-described manufacturing method may be dried in a drying chamber, pulverized into fine powder, packaged, and then distributed.

이와 같이 된장과 고추장을 분말로 제조하여 유통시키는 경우에는 유통고장에서 된장이나 고추장이 과도하게 발효 숙성되는 것을 방지할 수 있는 것이므로 유통기간을 상당기간 연장시킬 수 있는 등의 이점이 있는 것이다.In the case of distributing soybean paste and red pepper paste in powder as described above, it is possible to prevent excessive fermentation and fermentation of doenjang or red pepper paste in distribution failure, so that the distribution period can be extended for a considerable period of time.

이상에서는 본 발명의 바람직한 실시예에 대하여 도시하고 또한 설명하였으나, 본 발명은 상기한 실시예에 한정되지 아니하며, 청구범위에서 청구하는 본 발명의 요지를 벗어남이 없이 당해 본 발명이 속하는 분야에서 통상의 지식을 가진 자라면 누구든지 다양한 변형 실시가 가능한 것은 물론이고, 그와 같은 변경은 기재된 청구범위 내에 있게 된다.Although the preferred embodiments of the present invention have been illustrated and described above, the present invention is not limited to the above-described embodiments, and the present invention is not limited to the above-described embodiments without departing from the spirit of the present invention as claimed in the claims. Various modifications can be made by those skilled in the art, and such modifications are intended to fall within the scope of the appended claims.

이상에서 상술한 바와 같은 본 발명은, 품종이 우수한 콩을 삶은 후 탄산칼슘을 투여하여 콩의 수분함유량과 산도를 적절하게 조절하고, 압력탱크에서 높은 열과 압력을 가하여 각종 유해세균들을 멸균시킨 후 액상의 상황버섯 균사체를 접종하여 충분히 발효시킨 콩을 소금물에 담근 후 숙성시키거나 녹말가루, 고춧가루, 물엿, 소금을 고르게 혼합하여 숙성시키면 영양분이 풍부하고 풍미가 뛰어난 된장 및 고추장을 제조할 수 있었고, 이와 같이 제조된 장류는 상황버섯의 뛰어난 효능과 된장이나 고추장이 갖는 발효식품의 효능이 서로 상충작용을 일으켜 항암 효과를 극대화시킬 수 있는 완전한 식품을 구현할 수 있는 것이므로 각종 암이나 골다공증, 심혈관 질환과 같은 다양한 질병을 예방할 수 있었을 뿐 아니라 암세포의 성장을 억제시키는 효과와 함께 암을 유발하는 돌연변이를 억제하여 암을 예방할 수 있는 최상의 발효 식품을 제공하여 국민의 건강을 증진시켜 줄 수 있는 등의 이점이 있는 것으로서 된장 및 고추장의 대외 경쟁력을 최대한 높여줄 수 있는 것이다.As described above, the present invention, after boiling soybean varieties excellently administered calcium carbonate to properly control the water content and acidity of the soybeans, by applying high heat and pressure in a pressure tank sterilized various harmful bacteria after liquid Soybean fermented with mushroom mycelium in fermented soybean fermented in salt water and then aged or evenly mixed with starch powder, red pepper powder, starch syrup, and salt to produce nutrient-rich and flavorful doenjang and red pepper paste. The prepared soybeans can produce a complete food that can maximize the anti-cancer effect due to the conflict between the excellent efficacy of the situation mushroom and the fermented foods of doenjang or red pepper paste, so that various kinds of cancers, osteoporosis, cardiovascular diseases, etc. Not only prevented the disease but also suppressed the growth of cancer cells As to suppress the mutations that cause cancer offers the best fermented foods can prevent cancer with which the advantages of such can give to enhance people's health is to give maximizes the competitiveness of soybean paste and red pepper paste.

Claims (3)

품종이 우수한 양질의 콩을 선별하여 정화된 깨끗한 물로 세척하는 단계;Selecting high quality soybeans and washing them with clean water; 세척된 콩을 80℃의 온수에 담그고, 2시간 동안 침지시켜 콩을 불리는 단계;Soaking the washed beans in warm water at 80 ℃, soaking for 2 hours to call the beans; 불려진 콩을 탱크에 투입하여 함께 주입된 물을 끓이거나 고열의 스팀을 부여하여 20분간 삶아서 콩을 팽화(膨化)시키는 증자단계;A steaming step in which the soaked beans are put into a tank to boil the water injected together or given high heat steam to boil for 20 minutes to swell the beans; 삶아진 콩에 물기를 제거한 후 탄산칼슘을 고르게 투여하여 콩의 수분함유량이 50%를 유지토록 하고, 수소이온농도(pH)는 6.5가 유지되도록 하여 약산성으로 조절하는 콩의 수분 및 산도의 조절단계;After removing the water from the boiled soybeans, calcium carbonate is evenly administered to maintain the water content of the soybeans to maintain 50%, and the hydrogen ion concentration (pH) is maintained at 6.5 to adjust the moisture and acidity of the soybeans to be slightly acidic. ; 수분 및 산도가 적절하게 조절된 콩을 1.5기압과 130℃를 유지하는 압력탱크 내부에 투입하여 1시간 30분간 압력과 열을 가하여 각종 유해세균들을 멸균시키는 단계;Sterilizing various harmful bacteria by applying pressure and heat for 1 hour and 30 minutes in a pressure tank maintained at 1.5 atm and 130 ° C., so that the moisture and acidity are properly adjusted; 멸균된 콩을 식혀서 냉각시키는 단계;Cooling the sterile beans by cooling; 증자되어 팽화된 콩을 접종실에 투입하여 액상의 상황버섯 균사체를 고르게 분무하여 종균을 접종하는 단계;Injecting the increased and expanded bean into the inoculation chamber to inoculate the spawn by spraying evenly the liquid situation mushroom mycelium; 종균의 접종이 완료된 콩을 배양실의 온도가 22∼25℃를 유지한 상태에서 15일간 배양하여 콩을 발효시키는 단계;Fermenting the soybeans by incubating the soybeans which have been inoculated with the spawn seed for 15 days while maintaining a temperature of 22 to 25 ° C. in the culture chamber; 발효된 콩을 소금물에 담그어 1∼3개월 숙성(aging)시키는 단계에 의하여 된장이 완성됨을 특징으로 하는 상황버섯 균사체를 이용한 된장의 제조방법.Method of producing doenjang using the situation mushroom mycelium characterized in that the miso is completed by immersing the fermented soybeans in brine for 1 to 3 months. 품종이 우수한 양질의 콩을 선별하여 정화된 깨끗한 물로 세척하는 단계;Selecting high quality soybeans and washing them with clean water; 세척된 콩을 80℃의 온수에 담그고, 2시간 동안 침지시켜 콩을 불리는 단계;Soaking the washed beans in warm water at 80 ℃, soaking for 2 hours to call the beans; 불려진 콩을 탱크에 투입하여 함께 주입된 물을 끓이거나 고열의 스팀을 부여하여 20분간 삶아서 콩을 팽화(膨化)시키는 증자단계;A steaming step in which the soaked beans are put into a tank to boil the water injected together or given high heat steam to boil for 20 minutes to swell the beans; 삶아진 콩에 물기를 제거한 후 탄산칼슘을 고르게 투여하여 콩의 수분함유량이 50%를 유지되도록 하고, 수소이온농도(pH)가 6.5정도를 유지토록 하여 약산성으로 조절하는 콩의 수분 및 산도의 조절단계;After removing water from the boiled beans, calcium carbonate is evenly administered to maintain the water content of the soybeans to maintain 50%, and the hydrogen ion concentration (pH) is maintained at about 6.5 to control the moisture and acidity of the soybeans to be slightly acidic. step; 수분 및 산도가 적절하게 조절된 콩을 1.5기압과 130℃를 유지하는 압력탱크 내부에 투입하여 1시간 30분간 압력과 열을 가하여 각종 유해세균들을 멸균시키는 단계;Sterilizing various harmful bacteria by applying pressure and heat for 1 hour and 30 minutes in a pressure tank maintained at 1.5 atm and 130 ° C., so that the moisture and acidity are properly adjusted; 멸균된 콩을 식혀서 냉각시키는 단계;Cooling the sterile beans by cooling; 증자되어 팽화된 콩을 접종실에 투입하여 액상의 상황버섯 균사체를 고르게 분무하여 종균을 접종하는 단계;Injecting the increased and expanded bean into the inoculation chamber to inoculate the spawn by spraying evenly the liquid situation mushroom mycelium; 종균의 접종이 완료된 콩을 배양실의 온도가 22∼25℃를 유지한 상태에서 15일간 배양하여 콩을 발효시키는 단계;Fermenting the soybeans by incubating the soybeans which have been inoculated with the spawn seed for 15 days while maintaining a temperature of 22 to 25 ° C. in the culture chamber; 발효된 콩을 건조실에 투입하여 수분함유량 10%이하로 건조시키는 단계;Adding the fermented soybeans to a drying chamber to dry the water content below 10%; 건조된 콩을 분쇄기에 투입하여 고운 분말로 분쇄하는 단계;Pulverizing the dried beans into a fine powder; 분쇄된 콩 분말에 녹말가루, 고춧가루, 물엿, 소금을 고르게 혼합하여 물과 함께 버무리는 혼합단계;A mixing step of mixing together with water by mixing starch powder, red pepper powder, starch syrup and salt with crushed soybean powder; 버무려진 반죽물을 3개월 이상 숙성시키는 단계에 의하여 고추장이 완성됨을 특징으로 하는 상황버섯 균사체를 이용한 고추장의 제조방법.Method for producing red pepper paste using the situation mushroom mycelium characterized in that the red pepper paste is completed by the step of aging the kneaded dough 3 months or more. 제1항과 제2항에 의하여 제조된 된장과 고추장을 건조실에서 각각 건조시키고, 고운 분말로 분쇄하여 포장한 후 유통시킴을 특징으로 하는 상황버섯 균사체를 이용한 된장 및 고추장의 제조방법.The method for producing doenjang and red pepper paste using the situation mushroom mycelium, characterized in that the dried doenjang and red pepper paste prepared according to claim 1 and 2 are dried in a drying chamber, pulverized and packaged into fine powder.
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101236113B1 (en) * 2010-07-09 2013-02-21 김세영 Method for manufacturing raw powder of red pepper paste
KR101334412B1 (en) * 2012-03-14 2013-11-29 김동유 The method of preparing powder-enzyme using cereal's buds for soybean sauce
KR101478910B1 (en) * 2013-06-04 2015-01-08 전남대학교산학협력단 Soybeans for soybean paste and manufacturing method thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101236113B1 (en) * 2010-07-09 2013-02-21 김세영 Method for manufacturing raw powder of red pepper paste
KR101334412B1 (en) * 2012-03-14 2013-11-29 김동유 The method of preparing powder-enzyme using cereal's buds for soybean sauce
KR101478910B1 (en) * 2013-06-04 2015-01-08 전남대학교산학협력단 Soybeans for soybean paste and manufacturing method thereof

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