KR20040056080A - Cosmetic composition for preventing and therapeutic for a pimple - Google Patents

Abstract

PURPOSE: Provided is a cosmetic composition for preventing and treating a pimple using Poygonum tinctorium Lour extract. The composition has excellent antibacterial effect on Propionibacterium acnes causing a pimple, and antioxidation activity. CONSTITUTION: A cosmetic composition for the prevention and treatment of a pimple is characterized by containing 0.001-20.0 wt.% of Poygonum tinctorium Lour extract, based on the dried weight of the composition. The extract of Poygonum tinctorium Lour is obtained by the steps of: drying and pulverizing Poygonum tinctorium Lour; extracting it with a solvent; filtering the extract; distilling the filtrate under reduced pressure and concentrating it; and freeze-drying the concentrate and pulverizing it.

Description

Cosmetic composition for preventing and treating acne containing Namsil extract {Cosmetic composition for preventing and therapeutic for a pimple}

The present invention relates to a cosmetic composition having an acne preventive and therapeutic action, and more particularly, excellent antibacterial action against propionibacterium acnes known as a causative agent for worsening acne, and also present on the skin surface. The present invention relates to a cosmetic composition containing a substance having excellent antioxidant power, which can reduce peroxidation of sebum components and degeneration of the product due to skin irritation and atmospheric contact.

Acne is an acronym for vulgar acne, which refers to the inflammation of many hair follicles on the face, especially the cheeks and forehead of puberty. Some may develop on the chest or back, and some may develop at age 40. Initially, the hair follicles are full of cortex and keratin, resulting in yellowish lumps, sometimes with blackened peaks. When you squeeze the cheese will come out. It is then inflamed and reddish. When a bacterium is infected, it is concentrated and turns yellow. The better spot is a crater-shaped trail. Usually these four kinds are mixed. The cause is due to the infection of bacteria in the sebum excessively secreted by the action of innate qualities and sex hormones, especially male hormones. Women 22 years and older often worsen just before menstruation. The cause of acne is not yet clear, but it can generally be summarized as an increase in sebum secretion, keratinization and peroxidation of lipid components, proliferation of acne-causing bacteria, and inflammation and exacerbation. One of the important features related to the development of acne is the increase in sebum secretion, which is known to be closely related to the androgen, a male hormone. The secretion of male hormones above normal levels promotes sebaceous gland function, and the excess sebum is stagnated in the hair follicles due to the keratinization of the hair follicle wall, creating scleroderma. Sebum accumulated in the hair follicles, which cannot be discharged to the outside, blocks the pores and prevents the inflow of external air, so the inside of the hair follicles becomes an environment where propionibacterium acnes, which are anaerobic bacteria, can proliferate smoothly. Propionibacterium acnes secrete lipolytic enzymes and chemical factors to produce free fatty acids, trigger the immune response of leukocytes, and destroy the hair follicle wall, causing the contents to leak into the dermis, resulting in inflammatory reactions. . Therefore, the general treatment for acne can be summarized into four cases: suppression of excessive sebum production, inhibition of keratinization of the hair follicle wall, inhibition of proliferation of propionibacterium acnes, and minimization or inhibition of inflammatory response. .

It's a good idea to treat these acne quickly, but wash your face in the mornings and evenings, and use cosmetics such as astringent lotions, varnishing creams, foundations, twin cakes, or cold creams. If you have a disorder or constipation, give a fundamental treatment. Avoid mental stress and eat mainly beef, fish and vegetables. As an external medicine, cummerfeld liquid has been used for a long time, and sulfa antibiotics, vitamins A, B2, B6, and hormones are used. However, these formulations have side effects such as skin rashes, dry skin, and allergies may occur depending on the individual. Salicylic acid, in particular, is low in effectiveness and may worsen acne due to side effects such as skin rash. Hormonal agents have the disadvantage of increasing capillaries and dilating pores. In oriental medicine, acne is regarded as a chronic disease caused by weakened skin immunity due to dysfunction of the circulatory system and autonomic nerves. As a treatment for this, it is understood that the most basic method is to restore the immune function and blood circulation of the skin to the normal state based on the basic principle of clearing detoxification (毒 解毒). However, the above medicine has the disadvantage of internal medicine. Therefore, while there is an advantage of fundamentally blocking acne by treating internal organs and improving the constitution, it is not only easy to heal long-term constitution problems with short-term oral medicine, but even if such problems are solved, the onset, advanced acne and other Problems with skin diseases often remain intact. In other words, the topical medicine used directly on the skin is more effective considering various aspects such as the speed and economic efficiency of the effect.

Accordingly, an object of the present invention is to provide a composition that can easily prevent and treat acne by solving the above-described disadvantages of conventional acne treatments.

Another object of the present invention is to provide a cosmetic composition that can be used in the form of a cosmetic composition for preventing and treating acne.

Furthermore, another object of the present invention is to provide a manufacturing method that can easily prepare the main components of the composition for preventing and treating acne.

The present inventors have performed a number of studies to achieve the above object of the present invention, as a result, has an excellent antibacterial action against propionibacterium acnes acting as the main causative agent of acne worsening, It has excellent antioxidant power and can be expected to suppress sebum peroxidation and alleviation of keratinization, and to find natural products containing substances with high safety even after long-term use and to effectively extract active ingredients from them, and to provide compositions containing them By completing the present invention.

One configuration for achieving the above object of the present invention has excellent antibacterial action against propionibacterium acnes acting as the main causative agent of acne exacerbation, leaving a material with high safety even after long-term use ( Persicaria tinctoria H.GROSS) is used to extract, the extract of Namsil containing 0.001% to 20.0% by weight on a dry weight basis, the prevention of acne is added to the cosmetic composition that can be added to the conventional And it provides a cosmetic composition having an effect effective for treatment.

Namsil, the main component of the present invention as described above, is a yearly herbaceous plant of the Eurasian family, commonly called the side, also called the cradle to resemble the Eternal side and the stem and leaves of the side is called Cheongdae. Namsil, which is native to China, is brought in as a dye source and grown on farms. The height is 50 ~ 60cm and the stem is reddish purple. Red flowers bloom in August-September to form water inflorescences, which run densely at the end of the main stem. Fruits ripen in September-October, achenes are surrounded by flower cover, and are 2mm long and dark brown. Since ancient times, the leaves of Namsil contain indigo, which is an indigo dye, and it was used as a dye. Cheongdae was also used as a beauty paint for eyebrows. Namsil has excellent clearing detoxification action to reduce heat, digestion, detoxification, and bactericidal action has been used as an antidote for digestive disorders and addiction of all drugs, or have been used to treat chronic eczema and contact dermatitis.

In addition, Namsil of the present invention is a plant that has been used in herbal medicine for a long time, even in the case of ingestion of the body, the stability of the human body is confirmed, and there is little irritation to the skin and mucous membranes, and the plant is excellent in safety.

Korean Patent Laid-Open No. 2000-6570 discloses a bioactive extract containing raw ethyl acetate solubles. However, there is no mention of antimicrobial effects such as inhibition of propionibacterium acnes by these ethyl acetate extracts.

The content of Namsil extract in the acne prevention and treatment composition according to the present invention contains 0.001% to 20.0% by weight on a dry weight basis, preferably 0.005% to 10.0% by weight. If the amount is less than 0.005% by weight, the antimicrobial activity against propionibacterium acnes may be somewhat insufficient, and when more than 10.0% by weight is used, it is difficult to prepare the composition and ensure long-term stability. In addition, the concentration of less than 0.005% by weight of the antioxidant power is low, it is difficult to expect the peroxidation inhibitory effect of the sebum component, so the efficacy of the present skin composition is halved.

Namsil extract-containing skin composition of the present invention as described above can be applied to any shape, such as liquid, cream, paste or solid form cream, lotion, skin lotion, pack, foundation, makeup base as well as soap, facial foam, body It is also applicable to facial products such as cleansers, cleaning agents, and hair products such as shampoos and rinses, and, as necessary, ingredients commonly used in external preparations for skin within the scope of not impairing the effects of the present invention, for example, aqueous components, oily substances. It is possible to mix | blend with a component, surfactant, a humectant, a thickener, a preservative, a fragrance | flavor, a pigment | dye, etc.

Moreover, it can also be used together with antibacterial agents, such as benzoyl peroxide, tetracycline, erythromycin, and triclosan, hormones, such as androgens, steroids, and vitamin A derivatives, such as a retinoid, which have been used for conventional acne treatments.

According to another configuration of the present invention, Namsil extract of the present invention is

a) powdering dried male thread;

b) extracting the powdered male thread with a solvent;

c) filtering the extract filtrate;

d) concentration of the filtrate by distillation under reduced pressure; And

e) freezing and drying the concentrated filtrate to powder again.

According to another configuration of the present invention, the solvent used in the extraction step is purified water, methanol, ethanol, propanol, isopropanol, butanol, acetone, glycerol, propylene glycol, 1,3-butylene glycol, methyl acetate, ethyl acetate , At least one selected from the group consisting of hexane, diethyl ether and dichloromethane.

In particular, it is preferable to use a polar solvent such as purified water, methanol, ethanol, acetone, glycerol, propylene glycol, 1,3-butylene glycol in the extraction solvent. Namsil extract obtained using this extract solution has a strong antimicrobial effect against propionibacterium acnes, and excellent antioxidant power can alleviate the peroxidation and keratinization of sebum components. Meanwhile, Namsil extracts obtained using nonpolar solvents such as hexane, diethyl ether, and dichloromethane also exhibit antimicrobial and antioxidant effects against propionibacterium acnes, but are relative to extracts using polar solvents. It is a low side, and the solubility and ease of emulsification is poor, it is not preferable because the difficulty in preparing the composition for the skin.

Hereinafter, the present invention will be described in detail through Examples and Comparative Examples. However, these examples are only for illustrating the present invention and the present invention is not limited thereto.

Example 1

Extract of active ingredient of Namsil

100 ml of crushed Namsil (dried herb) powder was added to 1000 g of methanol (80%), which was immersed at room temperature for 1 week, followed by repeated extraction and filtration twice. The filtrate was concentrated by distillation under reduced pressure, and freeze-dried again. 18.6 g of powder were obtained.

Test Example 1

Determination of Antimicrobial Activity

Antibacterial activity is measured by a broth dilution method using a 96-well microplate. Heat-sterilized Brainheart Infusion Broth medium (BHI broth) is injected into each well of a 96-well microplate, followed by two-fold dilution of the extract sample. After injecting the test cell suspension into all wells, the cells are cultured anaerobicly at 37 ° C. After incubation for 3 to 4 days, the alamar blue solution is dispensed into each well and incubated for about 1 hour, and then the color development is observed to determine the concentration of the color change from blue to pink as the MIC value. .

Antimicrobial Activity against Propionibacterium acnes sample MIC (ppm) ATCC 6919 Namsil Extract 10 to 30 Triclosan 20 to 40 Salicylic acid 2000

As shown in the table, Namsil extract showed a very good antibacterial activity compared to benzoyl peroxide, which has been used as an active ingredient in conventional acne treatments, and showed a comparable or superior effect even when compared to triclosan, which is known to have a wide range of antibacterial activity. Therefore, when the extract of Namsil of the present invention is formulated into a composition for skin for the purpose of preventing and treating acne, it is expected to exhibit an excellent preventive and healing effect.

Test Example 2

Measurement of antioxidant effects

After dissolving the extract sample in an appropriate concentration in ethanol, a sample solution of various concentrations is prepared through step dilution. 50 μl of each sample solution is added to each well of a 96-well microplate, and then 100 μl of DPPH (1,1-Diphenyl-2-picrylhydrazyl) solution dissolved in ethanol at 20 mM concentration again. The change in absorbance is measured at 517 nm over a fixed time period. The reaction was carried out for 30 minutes, and the concentration of the sample showing 50% of the initial absorbance was expressed as a value of SC ₅₀ , and the antioxidant effect was evaluated using this.

Antioxidant Effect through DPPH Radical Scavenging Activity sample SC ₅₀ (517 nm.ppm) Namsil Extract 132.7 Butylene hydroxy toluene (BHT) 243.3

As shown in the table, Namsil extract was found to have a markedly superior antioxidant effect compared to BHT that has been commonly used as an antioxidant. Based on the above results, when using the extract of Namsil in combination with the skin composition, it is expected not only to suppress the peroxidation and keratinization of lipid components, but also to improve the safety of the skin and the phase stability of the composition.

Test Example 3

Evaluation of Antimicrobial Activity of Extracts Containing Namsil Extract

In order to evaluate the antimicrobial effect of the male-containing composition according to the present invention, the facial soaps of Example 2 and Comparative Example 1 were prepared using the composition shown in Table 3, and the paper-disk method was used. After diluting each composition with a 10% solution, the paper-disk (diameter 8 mm) was immersed in the solution for the same time, and then taken out and placed on an agar plate inoculated with the test bacteria and incubated. After incubation for 1 to 2 days, the antimicrobial effect of the composition was qualitatively determined by measuring the presence and absence of the development zone around the paper-disk, and the results are shown in Table 4.

Composition of face wash soap for measuring the effect (% by weight) Name Example 2 Comparative Example 1 Palm oil fatty acid 2.0 2.0 glycerin 0.5 0.5 Titanium dioxide 0.3 0.3 EDTA-2Na 0.1 0.1 Spices Quantity Quantity Color Quantity Quantity Namsil Extract 1.0 - Soap Chips (Base) Remaining amount Remaining amount Sum 100 100

Antimicrobial Activity against Propionibacterium acnes sample Diameter of growth inhibition zone (mm) ATCC 6919 Example 1 (10% solution) 16 Comparative Example 1 (10% solution) Lowland not formed

As a result of evaluating the antimicrobial effect on the dilute solution of the face-wash soap containing Namsil extract of the present invention, it showed a very excellent effect as shown in the [Table 4]. According to these results, it is possible to expect a preventive and therapeutic effect on the acne of the skin composition containing the extract of the present invention, namely, facial soap.

Test Example 4

Evaluation of Acne Treatment Effects

In order to evaluate the clinical effect of the acne prevention and treatment of acne-containing composition according to the present invention, the skin lotion of Example 3 and Comparative Example 2 was prepared with the composition as shown in Table 6, and tested for acne holders. In other words, for 40 women in their 20s with acne symptoms, the skin lotion having the composition shown in Table 6 was used twice a day for 3 weeks, and the condition of acne was classified according to the criteria of Table 5, and compared by visual and photographing. It judged through and the result is shown in Table 7.

Rating Skin condition 0 normal One Small number of cotton veins 2 Medium cotton patch without inflammation 3 Scalp of inflammatory papules 4 Small nodules and inflammatory scalp 5 Papular nodules 6 Moderate inflammatory nodules 7 Large number of inflammatory nodules 8 Severe nodular scalp 9 Severe cystic and inflammatory nodules

※ If the skin condition of acne in clinical trial subjects showed more than 3 grade reduction compared with the beginning of the experiment, it is excellent. If 1 ~ 2 grade is decreased, it is good. If there is no difference between grades, it is judged as worse. It was.

Composition of skin lotion for measuring effect (% by weight) Name Example 3 Comparative Example 2 ethanol 5.0 2.0 glycerin 3.0 0.5 1,3-butylene glycol 3.0 0.3 Polyoxyethylene alkyl ether 0.5 0.1 Spices Quantity Quantity Namsil Extract 1.0 - Purified water Remaining amount Remaining amount Sum 100 100

Evaluation of Acne Treatment Effects division Grading system Great Good no effect worse Example 2 14 (70%) 5 (30%) 1 (5%) - 20 (100%) Comparative Example 2 - 2 (10%) 15 (75%) 3 (15%) 20 (100%)

Acne prevention and treatment effect of the composition according to the present invention was found to have a very good effect as shown in Table 7.

Example 4-8

Hereinafter, the composition of the various types of cosmetic composition containing a Namsil extract obtained according to Example 1 of the present invention is shown in Table 8-12.

Cream (% by weight) Stearic acid 8.0 glycerin 5.0 Squalene 5.0 Propylene glycol 3.0 Self-emulsifying glycerin monostearate 2.5 Monostearic acid polyoxyethylene sorbitan (20 E.O.) 1.5 Vaseline 1.5 Spices Quantity antiseptic Quantity Namsil Extract 1.0 Purified water Remaining amount Sum 100

Lotion (% by weight) Liquid paraffin 5.0 glycerin 3.0 Squalene 3.0 Propylene glycol 3.0 Vaseline 2.0 ethanol 2.0 Monostearic acid polyoxyethylene sorbitan (20 E.O.) 1.5 Triethanolamine 0.5 Spices Quantity antiseptic Quantity Namsil Extract 1.0 Purified water Remaining amount Sum 100

Liquid foundation (% by weight) Titanium dioxide 8.0 Myristic acid isopropyl 5.0 Talc powder 5.0 Liquid lanolin 3.0 Liquid paraffin 3.0 glycerin 3.0 Bentonite 0.5 Cetostearyl alcohol 0.5 Carboxymethyl Cellulose Sodium 0.3 Coloring pigment Quantity Spices Quantity antiseptic Quantity Namsil Extract 1.0 Purified water Remaining amount Sum 100

Essence (% by weight) Sorbitol 8.0 Polyoxyethylene glycol 1500 6.0 ethanol 5.0 glycerin 3.0 1,3-butylene glycol 3.0 Polyoxyethylene oleyl alcohol ether 1.0 olive oil 0.3 Hyaluronic acid 0.2 Spices Quantity antiseptic Quantity Namsil Extract 1.0 Purified water Remaining amount Sum 100

Cleansing Foam (% by weight) Myristic acid 15.0 Stearic acid 12.0 Potassium hydroxide 5.0 glycerin 3.0 1,3-butylene glycol 3.0 Monostearic acid polyoxyethylene sorbitan (20 E.O.) 2.0 EDTA-2Na 0.1 Pigment Quantity Spices Quantity antiseptic Quantity Namsil Extract 1.0 Purified water Remaining amount Sum 100

As described above, the method of extracting the active ingredient of Namsil according to the composition of the present invention and the cosmetic composition containing the same not only show an excellent effect on the prevention and treatment of acne, but also the peroxidation of the sebum component present on the skin surface and the skin irritation and the atmosphere. Provided is a method for easily obtaining an antioxidant active ingredient having excellent antioxidant power, which can reduce the denaturation of the product by contact, and provides a cosmetic composition that contains these extracts in a proportion to prevent acne or to treat with excellent effects. It is a useful invention.

Claims (5)

A cosmetic composition for preventing and treating acne, characterized in that it contains an extract of Persilaria tinctoria H. GROSS, and a conventional cosmetic composition is added thereto. The cosmetic composition for preventing and treating acne according to claim 1, wherein the extract of Namsil contains 0.001% to 20.0% by weight on a dry weight basis. The cosmetic composition according to claim 1 or 2, wherein the cosmetic material is a cream, lotion, skin lotion, pack, foundation, essence, cleansing foam, soap, facial foam, or body cleanser. a) powdering dried Persimaria tinctoria H. GROSS; b) extracting the powdered male thread with a solvent; c) filtering the extract filtrate; d) concentration of the filtrate by distillation under reduced pressure; And e) a method of producing Namsil extract, characterized in that it is prepared by a method comprising the step of lyophilizing the concentrated filtrate to powder again. The method of claim 4, wherein the extraction solvent is purified water, methanol, ethanol, propanol, isopropanol, butanol, acetone, glycerol, propylene glycol, 1,3-butylene glycol, methyl acetate, ethyl acetate, hexane, diethyl ether, dichloro A method for producing Namsil extract, characterized in that at least one from the group consisting of methane.