KR102524746B1 - How to make herbal composition for improving acne - Google Patents

Abstract

The present invention relates to a method for manufacturing an herbal composition for alleviating acne. In addition, according to the present invention, a method for manufacturing an herbal composition for alleviating acne containing active ingredients effective for acne skin using a mixed extract containing an herbal composition is provided. The present invention relates to the method for manufacturing the herbal composition for alleviating acne comprising: (a) a raw material preparation step; (b) a raw material grinding step of freezing the raw materials at a temperature of -200℃ to -50℃ and grinding the same to an average particle size of 10 to 100 mesh; (c) a drying step of drying the pulverized raw materials at a temperature of 50℃ to 65℃ for 10 to 15 hours; and (d) an extraction step of extracting the dried raw materials.

Description

How to make herbal composition for improving acne}

The present invention relates to a method for manufacturing an herbal composition for improving acne, and more particularly, to a method for manufacturing an herbal composition for improving acne containing an active ingredient effective for acne skin using a mixed extract containing the herbal composition.

Acne is a polymorphic skin disease that appears in the form of cotton wool, pustules, papules, and nodules on the face, chest, and shoulders, and mainly occurs during adolescence with secondary maturation. It has been reported that about 80% of cases occur between the ages of 11 and 30, and about 6% of patients after the age of 30 have been reported, but recently, the incidence of adult acne due to stress is increasing. This disease is unpleasant to the patient and can cause aesthetic damage due to scarring and skin pigmentation. Accordingly, interest in the prevention and treatment of acne is increasing.

Since the exact cause of the occurrence of acne has not been identified and has various etiologies in terms of physiological and environmental aspects, it is difficult to appropriately treat acne that occurs in various types. In general, complex factors such as excessive sebum secretion from the sebaceous glands, hormonal imbalance, genetic predisposition, abnormal keratosis in the hair follicle, proliferation of Propionibacterium acnes, and inflammatory reactions are known to be the causes (Farrar, M. D. and Ingham, E. Acne: Clin. Dermatol. 2004. 22: 380-384; Kligman, A. M. J. Invest. Dermatol. 1974. 62: 268-287) Accordingly, as a basic method for treating acne, correcting the change in character due to keratinization of the hair follicle and reducing the activity of the sebaceous gland , antibacterial action against major strains causing acne, and skin anti-inflammatory action are applied.

Staphylococci and Micrococci, which are aerobic bacteria, and equivalents belonging to the genus Propionibacterium, which are anaerobic bacteria, reside on normal skin, and acne may occur due to the activity of these strains. In particular, Propionibacterium acnes, an anaerobic strain with lipophilicity, is pointed out as the main causative agent of acne (Burkhart CG et al., Postgrad. Med. J. 1999 75: 328). P. acnes hydrolyzes triglycerides of sebum produced by the male hormone androgen or other factors, then produces free fatty acids through the secretion of lipase and chemoattractant factors, causing an inflammatory response. . As the inflammatory reaction progresses, hair follicle cells are destroyed, and finally, the inflammatory reaction progresses to the dermis. In addition, secondary infection by other bacteria may occur after inflammation occurs.

The development of sebaceous glands, which is another cause of acne, occurs rapidly during the period of secondary sexual characteristics. When the secretion function becomes active due to the development of the sebaceous gland, sebum accumulates in the hair follicle and causes a rash. In addition, stagnant sebum blocks hair follicles and blocks air circulation, helping the growth of anaerobic bacteria such as P. acnes.

Hair follicle keratinization easily occurs in the dermal papilla, which is the end of the hair follicle, and the thickened keratin layer is peeled off in the hair follicle, resulting in closure of the dermal papilla, which forms a comedone. When the pores of the hair follicle or the opening of the sebaceous gland are blocked or narrowed by keratin, the normal discharge of sebum is suppressed and sebum stagnates in the dermal papilla. As a result, the proliferation of acne-related anaerobic bacteria increases, and the product stimulates the epithelial cells of the dermal papilla, resulting in more exfoliation (Baek Hye-yeon, Sookmyung Women's University master's thesis, Cosmetics, 2009. pp 7).

Acne treatment methods proposed so far include controlling sebum secretion using female hormones such as estrogen, an inhibitor of male hormones, or anti-androgenic agents, and removing dead skin cells using salicylic acid or retinoic acid, a vitamin A derivative. However, anti-inflammatory agents for suppressing inflammation or antibiotics for suppressing bacterial infection are most commonly used. However, when using conventional antibiotics such as tetracycline, erythromycin, and clindamycin, side effects such as the emergence of resistant bacteria, tissue and organ damage, and hypersensitivity reactions have emerged as a major problem. Accordingly, there is a need for research on antibacterial and anti-inflammatory properties for the treatment of acne by using natural substances that are less toxic and can reduce side effects.

An object of the present invention is to provide a method for preparing an herbal composition for improving acne.

In addition, another object of the present invention is to provide a method for preparing a herbal composition for improving acne containing active ingredients effective for acne skin by using a mixed extract containing the herbal composition.

According to one aspect of the present invention, in the method of manufacturing an herbal composition for improving acne, (a) a first raw material obtained by mixing one or two or more selected from turmeric root, magnolia bark, sorghum root, and centella asiatica root: , Eucommia, Sokdan, Deer Antler, Safflower In, Tosaja, Sukjihwang, Tortoiseshell, Cornus officinalis, Wolfberry, Licorice, Angelica, Ginger root, Gangjinhyang, Hwanpi, Sandpiper, and Goehwa selected as the second raw material. = 2.3~3.5: raw material preparation step of preparing mixed raw materials at a weight ratio of 1.3~1.8; (b) a raw material pulverization step of freezing the raw material at a temperature of -200 ° C to -50 ° C and pulverizing the raw material to an average particle size of 10 to 100 mesh; (c) a drying step of drying the pulverized raw material at a temperature of 50° C. to 65° C. for 10 to 15 hours; and (d) an extraction step of extracting the dried raw material.

The first raw material may be mixed and used in a weight ratio of turmeric root: magnolia bark: ginseng root: centella asiatica = 1.0: 1.2-1.5: 0.8-1.3: 1.0-1.5.

The second raw material may be used by immersing in an extract mixed in a weight ratio of Noni adventitious root extract: Eoseongcho extract = 0.8-2.1: 1.3-2.5 for 10 to 15 hours.

The (d) extraction step is to obtain an extract by putting the dried raw materials and solvent into a stirrer and repeating the process of stirring and extracting at room temperature (25 ± 2 ° C) at 150 rpm to 200 rpm for 12 to 24 hours three times to obtain an extract. Tea extraction step; A secondary extraction step of obtaining an extract by putting the dried raw materials and solvent into a low-temperature and high-pressure extractor and repeating the process of extracting for 1 hour to 3 hours at a pressure of 7.0 bar to 8.5 bar three times; Including, the first extraction step extract: the second extraction step extract = 0.5 ~ 0.8: can be used by mixing in a weight ratio of 1.3 ~ 1.5.

The herbal composition may be commercialized as any one or more of pharmaceutical compositions, cosmetic compositions, and food compositions.

According to the present invention as described above, it is possible to provide a method for manufacturing an herbal composition for improving acne.

In addition, according to the present invention, it is possible to provide a method for preparing an herbal composition for improving acne containing an active ingredient effective for acne skin using a mixed extract containing an herbal composition.

In addition, according to the present invention, the herbal composition prepared by the manufacturing method of the herbal composition for improving acne can be commercialized as medicines, cosmetics, and foods.

1 is a manufacturing process diagram of a manufacturing method of an herbal composition for improving acne of the present invention.
2 is a test result of fibroblast proliferation promoting activity.

Details of other embodiments are included in the detailed description and drawings.

Advantages and features of the present invention, and methods of achieving them, will become clear with reference to the detailed description of the following embodiments taken in conjunction with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but may be implemented in various different forms, and unless otherwise specified in the following description, components, reaction conditions, and content of components are expressed in the present invention. All numbers, values and/or expressions are to be understood as being qualified in all cases by the term "about" as such numbers are inherently approximations that reflect, among other things, various uncertainties of measurement that arise in obtaining such values. . Also, when numerical ranges are disclosed herein, such ranges are contiguous and include all values from the minimum value of such range to the maximum value inclusive, unless otherwise indicated. Furthermore, where such a range refers to an integer, all integers inclusive from the minimum value to the maximum value inclusive are included unless otherwise indicated.

Further, in the present invention, where ranges are stated for a variable, it will be understood that the variable includes all values within the stated range inclusive of the stated endpoints of the range. For example, a range of "5 to 10" includes values of 5, 6, 7, 8, 9, and 10, as well as any subrange of 6 to 10, 7 to 10, 6 to 9, 7 to 9, and the like. inclusive, as well as any value between integers that fall within the scope of the stated range, such as 5.5, 6.5, 7.5, 5.5 to 8.5 and 6.5 to 9, and the like. For example, a range of "10% to 30%" may range from 10% to 15%, 12% to 15%, as well as values such as 10%, 11%, 12%, 13%, and all integers up to and including 30%. It will be understood to include any sub-range, such as 18%, 20% to 30%, and the like, as well as any value between reasonable integers within the scope of the stated range, such as 10.5%, 15.5%, 25.5%, and the like.

In the present invention, the term "extract" refers to water, methanol, ethanol, lower alcohol having 1 to 4 carbon atoms such as butanol, methylene chloride, ethylene, acetone, hexane, ether, chloroform, ethyl acetate, butyl acetate, N, N- Extracts obtained by leaching using dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,3-butylene glycol, propylene glycol or a mixture thereof, and extracts obtained using supercritical extraction solvents such as carbon dioxide and pentane Or refers to a fraction obtained by fractionating the extract, and the extraction method may be applied by any method such as cooling, reflux, heating, ultrasonic radiation, supercritical extraction, etc. in consideration of polarity, extraction degree, and preservation degree of the active substance. In the case of fractionated extracts, including fractions obtained by suspending the crude extract in a specific solvent and then mixing and standing with solvents having different polarity, the crude extract is sequentially fractionated using the solvents in the order of increasing or decreasing polarity. and further includes fractions obtained by chromatography using properties such as size, charge, hydrophobicity, and affinity. In addition, the meaning of the extract may include a concentrated liquid or solid extract from which the extraction solvent is removed by lyophilization, vacuum drying, hot air drying, spray drying, or the like.

In addition, in the present invention, the meaning of the "active ingredient" means a component that exhibits the desired activity alone or that can exhibit activity in combination with a carrier having no activity itself.

Terms not specifically defined in the present invention follow the meanings or dictionary meanings commonly used in the art.

The composition of the present invention may contain the active ingredient in any amount (effective amount) as long as it can exhibit acne improvement activity, antioxidant activity or anti-inflammatory activity depending on the use, formulation, blending purpose, etc., but a typical effective amount is the entire composition. On a weight basis it will be determined within the range of 0.001% to 15% by weight. Here, "effective amount" refers to the amount of an active ingredient capable of exhibiting acne-improving activity, antioxidant activity, or anti-inflammatory activity in a mammal, preferably a human, to which it is applied. Such an effective amount can be determined empirically within the ordinary skill of the skilled artisan.

The present invention can provide a method for preparing an herbal composition for improving acne.

1 is a manufacturing process diagram of a manufacturing method of an herbal composition for improving acne of the present invention.

Referring to FIG. 1, the present invention is (a) a first raw material mixed with one or two or more selected from turmeric root, magnolia bark, ginseng root, and centella asiatica: , Tosaja, Sukjihwang, tortoise shell, cornus officinalis, goji berry, licorice, angelica root, kangjinhyang, joint hwanpi, sandalwood, and a mixture of two or more selected raw materials = 2.3~3.5: 1.3~1.8 A raw material preparation step of preparing raw materials mixed in a weight ratio (S100); (b) a raw material crushing step (S200) of freezing the raw material at a temperature of -200 ° C to -50 ° C and pulverizing the raw material to an average particle size of 10 to 100 mesh; (c) a drying step of drying the pulverized raw material at a temperature of 50° C. to 65° C. for 10 to 15 hours (S300); and (d) an extraction step (S400) of extracting the dried raw material.

The first raw material may be mixed and used in a weight ratio of turmeric root: magnolia bark: ginseng root: centella asiatica = 1.0: 1.2-1.5: 0.8-1.3: 1.0-1.5.

The second raw material may be used by immersing in an extract mixed in a weight ratio of Noni adventitious root extract: Eoseongcho extract = 0.8-2.1: 1.3-2.5 for 10 to 15 hours.

The noni adventitious root extract contains rubiadin, which is a major component, and the rubiadin is known to be effective in preventing and alleviating allergies and to have an antioxidant action.

The Eoseongcho extract is known to have excellent antibacterial and anti-inflammatory effects, and is also effective in anti-allergy, antioxidant, and immunity enhancement.

The (d) extraction step is to obtain an extract by putting the dried raw materials and solvent into a stirrer and repeating the process of stirring and extracting at room temperature (25 ± 2 ° C) at 150 rpm to 200 rpm for 12 to 24 hours three times to obtain an extract. Tea extraction step; A secondary extraction step of obtaining an extract by putting the dried raw materials and solvent into a low-temperature and high-pressure extractor and repeating the process of extracting for 1 hour to 3 hours at a pressure of 7.0 bar to 8.5 bar three times; Including, the first extraction step extract: the second extraction step extract = 0.5 ~ 0.8: can be used by mixing in a weight ratio of 1.3 ~ 1.5.

The herbal composition may be commercialized as any one or more of pharmaceutical compositions, cosmetic compositions, and food compositions.

Subjects to which the composition of the present invention can be applied (prescribed) are mammals and humans, particularly preferably humans.

In a specific aspect, the composition of the present invention can be identified as a cosmetic composition. In addition to the active ingredient, the cosmetic composition may include ingredients commonly used in cosmetic compositions, such as stabilizers, solubilizers, surfactants, vitamins, conventional adjuvants such as pigments and spices, and carriers. .

The cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , oil, powder foundation, emulsion foundation, wax foundation and spray may be formulated, but is not limited thereto. More specifically, it may be prepared in the form of softening lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, specifically water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol , 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan fatty acid ester, and the like.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Star cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the formulation of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide Ether sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

The cosmetic composition of the present invention may be prepared according to a method for preparing a cosmetic composition known in the art by including the active ingredient and the above-described carrier component.

The composition of the present invention can be conceived as a pharmaceutical composition in another specific aspect.

The pharmaceutical composition of the present invention includes a pharmaceutically acceptable carrier, excipient, etc. in addition to its active ingredient, and is formulated into topical formulations such as creams, lotions, ointments (semi-solid external medicines), microemulsions, gels, pastes, and transdermal preparations. (TTS) (eg patch, bandage, etc.) and the like.

In the above, "pharmaceutically acceptable" means that it does not inhibit the activity of the active ingredient and does not have toxicity more than is adaptable to the subject of application (prescription).

Examples of pharmaceutically acceptable carriers include lactose, glucose, sucrose, starch (eg corn starch, potato starch, etc.), cellulose, its derivatives (eg sodium carboxymethyl cellulose, ethyl cellulose, etc.), malt, gelatin, talc, solid lubricants (eg, stearic acid, magnesium stearate, etc.), calcium sulfate, vegetable oils (eg, peanut oil, cottonseed oil, sesame oil, olive oil, etc.), polyols (eg, propylene glycol, glycerin, etc.), alginic acid, and the like; may be used by selecting one or more suitable ones according to the formulation of the pharmaceutical composition of the present invention. For suitable pharmaceutically acceptable carriers and formulations, reference may be made to Remington's Pharmaceutical Sciences (19th ed., 199 5). The pharmaceutical composition of the present invention may further include an emulsifier (eg TWEENS), a wetting agent (eg sodium lauryl sulfate), a coloring agent, a flavoring agent, a stabilizer, a preservative, water, saline, a phosphate buffer solution, and the like.

Suitable excipients may be selected and used according to the formulation of the pharmaceutical composition of the present invention, for example, suspending or dispersing agents such as sodium carboxymethyl cellulose, methyl cellulose, hydropropylmethyl cellulose, sodium alginate, and polyvinylpyrrolidone. can

The daily dosage of the pharmaceutical composition of the present invention is usually in the range of 0.001 to 150 mg/kg body weight, and may be administered once or divided into several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in light of various related factors such as the route of administration, the age, sex, weight, and severity of the patient, the dosage limits the scope of the present invention in any aspect. should not be understood as

In a specific aspect, the composition of the present invention can be regarded as a food composition.

The food composition of the present invention may include sweeteners, flavors, physiologically active ingredients, minerals, and the like in addition to the active ingredients.

Sweeteners can be used in amounts that give the food a moderately sweet taste, and can be natural or synthetic. Preferably, natural sweeteners are used, and natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose, and maltose.

Flavors may be used to improve taste or aroma, and both natural and synthetic flavors may be used. Preferably, it is the case of using a natural one. In case of using natural ones, in addition to flavor, the purpose of enhancing nutrition can also be combined. As a natural flavoring agent, it may be obtained from apples, lemons, tangerines, grapes, strawberries, peaches, etc., or obtained from green tea leaves, roundworms, bamboo leaves, cinnamon, chrysanthemum leaves, jasmine, and the like. Also, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, and ginkgo can be used. Natural flavors can be liquid concentrates or solid extracts. In some cases, synthetic flavors may be used, and synthetic flavors may include esters, alcohols, aldehydes, terpenes, and the like.

As the physiologically active substance, catechins such as catechin, epicatechin, gallocatechin, and epigallocatechin, and vitamins such as retinol, ascorbic acid, tocopherol, calciferol, thiamine, and riboflavin may be used.

Examples of minerals include calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, silicon, sodium, sulfur, vanadium, zinc, and the like.

In addition, the food composition of the present invention may include a preservative, an emulsifier, an acidulant, a thickener, and the like, if necessary, in addition to the sweetener.

These preservatives, emulsifiers, etc. are preferably added and used in very small amounts as long as the purpose to which they are added can be achieved. A trace amount means a range of 0.0005% by weight to about 0.5% by weight based on the total weight of the food composition when expressed numerically.

Preservatives that may be used include sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate, EDTA (ethylenediaminetetraacetic acid) and the like.

Examples of emulsifiers that may be used include gum acacia, carboxymethylcellulose, xanthan gum, pectin, and the like.

Acidulants that may be used include acid salt, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, phosphoric acid, and the like. Such an acidulant may be added so that the food composition has a titratable acidity for the purpose of inhibiting the growth of microorganisms in addition to the purpose of enhancing the taste.

As thickeners that can be used, suspending agents, sedimentation agents, gel forming agents, bulking agents and the like may be mentioned.

Hereinafter, the present invention will be described with reference to Examples and Experimental Examples. However, the scope of the present invention is not limited to these Examples and Experimental Examples.

(Example) Preparation of herbal extract

As the first raw material, turmeric root, magnolia bark, sorghum root, and Centella asiatica root were mixed and prepared in a weight ratio of 1.0: 1.2: 0.8: 1.0, and corydalis was selected as the second raw material, and Noni adventitious root extract: Eoseongcho extract = 0.8: 1.3 After preparing by immersing in the extract mixed at a weight ratio for 12 hours, the first raw material: the second raw material was prepared by mixing at a weight ratio of 2.3: 1.3.

The mixed raw materials were frozen at -150 ° C, pulverized to an average particle size of 50 mesh, and then dried at 50 ° C for 12 hours.

The dried raw material was put into a stirrer with 70% ethanol, and the process of stirring and extracting at room temperature (25±2℃) for 20 hours at 150 rpm was repeated three times to obtain the primary extract, and the dried raw material was mixed with 70% ethanol. Into a low-temperature and high-pressure extractor together with, the process of extraction for 2 hours at a pressure of 8.0 bar was repeated three times to obtain a secondary extract. The first extract: the second extract = 0.7: mixed in a weight ratio of 1.5 to obtain the herbal extract of the present invention.

(Experimental Example 1) Antioxidant activity (DPPH radical scavenging ability measurement)

After preparing a DPPH solution by adding DPPH to 0.1 mM in 100% methanol, each sample in Example was 2% (w / v), 1% (w / v), 0.5% (w / v), 0.1% ( w/v) and 0.01% (w/v). After vortexing vigorously for 10 seconds and leaving it in the dark for 30 minutes, the absorbance is measured at 517 nm using ELISA. Antioxidant activity was expressed as percentage of DPPH radical scavenging ability based on the absorbance of the control group using pure water.

(%) 1st raw material Second raw material 1st raw material + 2nd raw material mixing process
density curcuma magnolia gosam Centella Asiatica Turmeric + Magnolia
+Gosam + Centella asiatica
mix corydalis Noni + Eoseongcho
immersion 2% 78 87 31 26 95 68 88 95 One% 79 87 27 19 95 62 88 96 0.5% 60 57 21 12 96 60 90 98 0.1% 21 53 21 9 88 58 86 93

Table 1 shows that the DPPH radical scavenging activity of the extract obtained by mixing the first raw material and the second raw material, that is, the herbal extract of the present invention according to the Example, was generally higher than that of the single extract of the first raw material and the single extract of the second raw material, This shows that the antioxidant activity is high. In particular, the treatment concentration of 0.1% (w/v) of the mixed extract showed a high antioxidant activity of 93% even at a low concentration.

(Experimental Example 2) Acne improvement activity - measurement of antibacterial activity against acne bacteria (Propionibacterium acnes)

Disc diffusion assay was used to measure antibacterial activity. Propionibacterium acnes, a test strain, was inoculated into 10 ml of liquid medium (GAM Broth) and subcultured 3 times for 24 hours at 37 ° C to be used as an antibacterial activity test strain. .

In the antibacterial activity test, the paper disc was inserted into the liquid medium (GAM Broth) in which the test strain was cultured, taken out, sufficiently wetted with the liquid medium in which the test strain was cultured, and each sample of the above example was 2 times using distilled water. 20 μl of the Paper Discs were added at concentrations of % (w/v), 1% (w/v), 0.5% (w/v), 0.25% (w/v), and 0.01% (w/v). did After incubation at 37 ° C. for 24 hours, the antibacterial activity was analyzed by measuring the size of the growth inhibition ring (clearzone, cm) generated around the disc. Distilled water used for diluting the extract was used as a negative control group.

1st raw material 2nd raw material 1st raw material + 2nd raw material mixing density
(%) curcuma magnolia gosam Centella Asiatica Turmeric + Magnolia
+Gosam + Centella asiatica
mix corydalis Noni + Eoseongcho
immersion 0% 0cm 0cm 0cm 0cm 0cm 0cm 0cm 0cm 0.25% 0cm 1.3cm 0cm 0cm 1.5cm 0cm 1.2cm 1.8cm 1.5% 0cm 1.5cm 0cm 0cm 1.8cm 0cm 1.5cm 2.2cm One% 0cm 2cm 0cm 0cm 2.2cm 0cm 2.2cm 2.5cm 2% 0cm 2.1cm 0cm 0cm 2.5cm 0cm 2.3cm 2.8cm

Looking at the results of Table 2, the extract obtained by mixing the first raw material and the second raw material, that is, the herbal extract of the present invention according to the embodiment has excellent antibacterial activity compared to the single extract of the first raw material and the single extract of the second raw material. .

(Experimental Example 3) Fibroblast proliferation promoting activity test (MTT assay)

Fibroblasts were counted using a hemocytometer at 1 Х 10 4 cells/well in the same way in a 96-well plate containing DMEM medium containing 0% FBS, then aliquoted and cultured for 48 hours. The herbal composition of the following examples was 0.1% (w / v), 0.05% (w / v), 0.01% (w / v), 0.005% (w / v), 0.001% (w / v) FBS- It was replaced with free DMEM and further cultured for 24 hours (sample dilution was diluted with culture medium). After incubation, 50 μl of 3-,4,5-di methylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide solution (2.5 mg/ml) was added, followed by further incubation for 3 hours, and the cell culture medium was completely removed. 200 μl of dimethyl sulfoxide was treated with 1 ml per well, stirred, and 100 μl was taken from 96 wells, and absorbance was measured at 570 nm by EnzymeLinked Immunosorbent Assay (ELISA).

The results are shown in Figure 2 below. 2 is a test result of fibroblast proliferation promoting activity. Referring to Figure 2, it can be seen that most of the fibroblasts proliferated in proportion to the treatment concentration.

(Experimental Example 4) Skin trouble improvement evaluation

In order to evaluate the improvement effect on skin acne trouble, 20 men and women (10 men, 10 women / 5 teenagers, 5 people in their 20s, 5 people in their 30s, 5 people in their 40s) were tested through an example. Cosmetics (lotions) containing the prepared herbal composition were applied to the troubled skin for 2 weeks, and sensory evaluation was performed according to the evaluation criteria in Table 3 below.

The evaluation criterion was evaluated as the average value of the subjects with a perfect score of 100 points.

Evaluation standard evaluation value Satisfaction with use 98 Acne skin improvement degree 99 Satisfaction with the moisturizing power of cosmetics 95 Improvement of wounds, etc. 88 flavor 70

Looking at the results of Table 3, it can be confirmed that the cosmetics (lotions) containing the herbal composition of the present invention prepared according to the examples received excellent evaluation in the overall satisfaction, acne improvement degree, moisturizing power, and wound improvement evaluation. there was.

Therefore, the herbal composition prepared by the manufacturing method of the herbal composition of the present invention can provide an effect of improving acne skin by improving anti-inflammatory and antioxidant effects by including various herbal ingredients.

Those skilled in the art to which the present invention pertains will understand that the present invention can be embodied in other specific forms without changing its technical spirit or essential features. Therefore, the embodiments described above should be understood as illustrative in all respects and not limiting. The scope of the present invention is indicated by the claims to be described later rather than the detailed description above, and all changes or modifications derived from the meaning and scope of the claims and equivalent concepts thereof are included in the scope of the present invention. should be interpreted

S100: raw material preparation step
S200: raw material crushing step
S300: Drying step
S400: Extraction step

Claims (5)

In the manufacturing method of the herbal composition for improving acne,
(a) First raw material that is a mixture of one or more selected from among turmeric root, magnolia bark, sorghum root and centella asiatica root: corydalis, sagebrush, eucalyptus, sokdan, antler, safflower, tosaja, sukjihwang, tortoiseshell, cornus officinalis, Goji berry, licorice, angelica root, kangjinhyang, haphwan husk, sandalwood, and a mixture of two or more selected raw materials = 2.3~3.5: raw materials to prepare mixed raw materials at a weight ratio of 1.3~1.8 preparatory stage;
(b) a raw material pulverization step of freezing the raw material at a temperature of -200 ° C to -50 ° C and pulverizing the raw material to an average particle size of 10 to 100 mesh;
(c) a drying step of drying the pulverized raw material at a temperature of 50° C. to 65° C. for 10 to 15 hours; and
Method for manufacturing an herbal composition for improving acne, comprising: (d) an extraction step of extracting the dried raw material. According to claim 1,
The first raw material,
Turmeric root: magnolia bark: sorghum root: Centella asiatica root = 1.0: 1.2-1.5: 0.8-1.3: 1.0-1.5 A method for producing an herbal composition for improving acne, characterized in that it is mixed and used in a weight ratio. According to claim 1,
The second raw material,
Noni adventitious root extract: Eoseongcho extract = 0.8 ~ 2.1: A method for producing an herbal composition for improving acne, characterized in that it is used by immersing for 10 to 15 hours in an extract mixed at a weight ratio of 1.3 to 2.5. According to claim 1,
The (d) extraction step,
A primary extraction step of obtaining an extract by putting the dried raw materials and solvent into a stirrer and repeating the process of stirring and extracting at room temperature (25 ± 2 ° C) for 12 to 24 hours at 150 rpm to 200 rpm three times to obtain an extract;
A secondary extraction step of obtaining an extract by putting the dried raw materials and solvent into a low-temperature and high-pressure extractor and repeating the process of extracting for 1 hour to 3 hours at a pressure of 7.0 bar to 8.5 bar three times; including,
The method for producing an herbal composition for improving acne, characterized in that the extract of the first extraction step: the extract of the second extraction step = 0.5 ~ 0.8: mixed in a weight ratio of 1.3 to 1.5. According to claim 1,
The herbal composition,
A method for preparing an herbal composition for improving acne that can be commercialized as any one or more of pharmaceutical compositions, cosmetic compositions, and food compositions.

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