KR20040018684A - composition of skin agent containing extract of Fumariaceae - Google Patents

Abstract

PURPOSE: Provided is a dermatological composition containing an extract of Fumariaceae as a main active ingredient. Therefore, it excellent anti-aging effect on the skin. CONSTITUTION: A dermatological composition is characterized by containing an extract of Fumariaceae, particularly Dicentra spectabisis, as a main active ingredient. The extract is obtained by using an extraction solvent selected from purified water, methanol, ethanol, glycerine, ethylacetate, butyleneglycol, propyleneglycol, dichloromethane and hexane.

Description

Composition for skin external preparation containing calendula and plant extract as main active ingredients {composition of skin agent containing extract of Fumariaceae}

The present invention relates to an external composition for skin containing the genus quince and plant extract as the main active ingredients, and more particularly, it contains 0.001-30.0% by weight of a bleeding extract, and an antioxidant effect, matrix metalloproteinase (matrix metallo) effective in inhibiting the activity of proteinase (hereinafter referred to as 'MMP'), especially collagenase (hereinafter referred to as "MMP-1") and gelatinase (hereinafter referred to as "MMP-2"). The present invention relates to a skin external composition having excellent expression inhibitory effect of MMP-1 and MMP-2 overexpressed in human fibroblasts.

Human skin is subject to various physical and chemical changes during the aging process, and the cause is largely divided into internal aging and photo-aging. Among the ultraviolet rays reaching the earth's surface from the sun, UVC is absorbed, scattered, and filtered in the ozone layer in the upper part of the atmosphere, so it does not affect the natural photochemical reaction much. Among the ultraviolet rays that directly affect the skin, the energy of UVB is about 8% of the UVA and biologically important because it is not completely filtered out of the ozone layer. UVB has a lot of effect on cell damage caused by sunburn. UVA also penetrates deep into the skin's epidermis and dermis, primarily from the sun, but also from artificial lamps.

Synthesis and degradation of extracellular matrix such as collagen is regulated in vivo, but the synthesis decreases as aging progresses, and the expression of matrix metalloproteinase (MMP), an enzyme that breaks down collagen, is promoted, resulting in decreased skin elasticity. And wrinkles are formed. Ultraviolet irradiation also activates these degrading enzymes.

Matrix metalloproteinases (MMPs) are calcium and zinc-dependent endopepes secreted from cells such as polymorphonuclearneutrophils, macrophage, gingival fibroblasts, bone cells Endopeptidase acts at neutral pH and uses various extracellular substrates as substrates. MMPs are broadly classified as MMP-1, MMP-2, and MMP-9 according to their substrate specificity. Fibroblast-type collagenase, an enzyme, includes collagen types I, II, III, VIII, VIII, VIII and gelatin. ) Facilitates the action. MMP-2 belongs to 72-kD gelatinase (A) and degrades gelatin, collagen types IV, V, VIII, VIII, elastin and fibronectin as substrates. Metallolastase also degrades elastin with MMP-13. Particularly, among the matrix metalloproteinases (MMP), type IV collagenase 72-kD and 92-kD collagenase are enzymes that break down type IV collagen, a major structural component of the basement membrane, the first barrier of cancer metastasis. It is the most important enzyme in infiltration and metastasis. The search and development of inhibitors against type IV collagenase has the function of finding and developing drugs useful for the treatment of rheumatoid, periodontal disease, and corneal ulcer caused by cancer invasion and metastasis and degradation of collagen connective tissue. .

The molecular structure of matrix metalloproteinases (MMPs) is generally characterized by five regions: signal peptides, propeptide, catalytic sites, hinge sites, and pepsin. Gelatinase, MMP-2 and MMP-9 have one fibronectin-like collagen site and MMP-9 additionally has another type V collagen site. These enzymes are secreted into the inactivated proenzymes and Zymogens, which are not stored in the cell and find activity as the signal peptide is lost. The function of the propeptide is involved in maintaining the inactivation of the enzyme and is cleaved in several steps. To induce the activity of these enzymes, detergents, oxidizing agents, organometallic substances and enzymes such as trypsin or prasmin are involved to release zinc from the enzyme catalyst site from cysteine, and the free zinc interacts with water to hydrolyze the substrate. Decompose Substrate metalloproteinases (MMPs) of neutrophils and polymorphonuclear leukocytes are primarily activated by oxidative processes. First, when hydrogen peroxide is generated in cells, it is induced by myeloperoxidase in the presence of chlorine ions. It is converted to chloric acid, which activates the substrate metalloproteinase (MMP). Matrix metalloproteinases (MMPs) in fibroblasts are activated by proteases such as trypsin and plasmin. Only a small amount of the activated enzyme performs a predetermined physiological function. When it is secreted and activated in excess, it causes various diseases mentioned above.

It is known that MMPs express genes by growth factors and cytokines to secrete enzymes. In mesenchymal and keratinocytes of mesodermal tissues, which are caused by blood vessels, connective tissues, and lymphatic vessels, cytokines such as growth factor and interleukin-1β, tumor necrosis factor-α, platelet-derived growth factor, and epidermal growth factor, While it promotes gene expression of (MMP), interferon-α and gluticocorticoids and transforming growth factor-β inhibit the gene expression of matrix metalloproteinases (MMPs). In addition, parathyroid hormone, endotoxin, and prostaglandin are known to increase the synthesis of matrix metalloproteinase (MMP) in relation to bone resorption. This genetic control mechanism shows that collagenase is synergistically modified by activator protein-1 for genes by synergistic transcription factors such as c-fos and c-jun, and additionally by the synergy of PEA3 and NIP protein binding sites. And maximal transcriptional activity in the stromelysin promoter. Neutrophil matrix metalloproteinase (MMP) regulation is primarily regulated by granulation of lysosomes rather than at the transcription level.

Looking at the patents related to the anti-aging of the skin, there is a cosmetic composition that promotes collagen synthesis or contains a peptide or plant extract as a collagenase inhibitor. In order to prevent skin aging, the conventional technique merely promotes collagen synthesis or inhibits the activity of collagenase, whereas the results of the study of bleeding heart extract have been shown to act on the substrate metalloproteinase to improve the antioxidant effect and skin wrinkle improvement. The same anti-aging efficacy has been found.

Dicentra spectabisis is a herbaceous plant belonging to the genus Dicentra, which is native to China. The plant is a perennial plant, 40 ~ 60cm high.

The whole is whiteish green, the stem is soft, standing upright and branching. The leaves are alternate, the petiole is long, and split into two pieces of three pieces. Cracked piece is wedge shaped like an upside down egg with pointed tip and edge at the edge. Flowers bloom pink in April-June, hanging on one side at the end of the main stem as a gunshot inflorescence. Corollas are convex pouches. Four petals gather to form a flat heart, and the two outer petals form a honey bag at the bottom. The two inner petals merge to form a tubular projection. Sepals are 2, thin, scaly, and fall early. There are six stamens, divided into two bodies, and one pistil. Fruits are long oval capsules. A white-flowered cultivar, D. spectabilis alba , whose leaves are deeply dug up into several small pieces, which are widely planted in other varieties of Dicentra. Bigger than them Widely planted varieties include D. eximia , which grows around Mount Elegeni in eastern North America, and blooms in small pink flowers from April to September. Growing in the Pacific coastal forests from California to British Columbia in the United States, there are many varieties planted as horticultural species in D. formosa .

Known by the elderly as stalks and vine peony, the plant reacts sensitively to soil, flowering in its original color in alkaline soil and red or white in acidic soil. Although it is a poisonous plant, young shoots are eaten as herbs and also used as herbs. The wide petals and flower sake resemble the mouth of a fish, creating an illusion of being underwater. In spring, young leaves are harvested and boiled as herbs. Herbal medicine is taken from the herbs and dried is called a golden sac (피), the blood is well selected and small (消腫) is effective for the treatment of bruises and boils. There are 0.1-2% alkaloids in the outposts and roots. The main ingredients are protopene and decentrin. Other copticins, kelerytrins, kelly rubins, and kelly rutin were also isolated. Alkaloids are 0.18% in the outpost and 1,22% in the roots. Protopene is 0.14% in the outpost and 1.01% in the roots. Dicentrin is anesthetic and causes cramps in large amounts. In addition, it is known that when injected into warm-blooded animals, fever lowering action is twice as strong as antipyrine (Ref., Korean Resource Plant).

Studies on Fuchsia Fumariaceae plants are described in U.S. Pat.No.511,6749 and U.S. Pat.No.4769452, but these inventions only separate useful enzymes from Fuchsia Fumariaceae plants. Oral medications that do not involve external preparations or those that act as transferases for specific enzymes.

The present invention is a method of selecting a calendula color and a plant extract from a variety of natural products, confirming the efficacy as a raw material of the external skin preparations, developing a method of using the same, and by using this raw material to produce a skin external preparation having a predetermined functionality The purpose is to provide.

That is, an object of the present invention is to provide a natural extract showing an excellent anti-aging effect such as antioxidant effect, skin wrinkle prevention effect by increasing the substrate metalloproteinase inhibitor and expression inhibitory function.

In another aspect, the present invention is to provide a method for producing a skin external preparation using the material as a skin external preparation raw material from the point that the target efficacy is more greatly selected when selecting a specific extraction solvent in the preparation of Hyunho colors and plant extracts and the skin external preparation.

In order to achieve the above object, the present invention is that the extract of the color of the cortex and plants using one or more solvents selected from purified water, methanol, ethanol, glycerine, ethyl acetate, butylene glycol, propylene glycol, dichloromethane and hexane It is to provide a topical skin composition containing the main color and the plant extract as a main active ingredient.

In another aspect, the present invention is to provide a skin external composition, characterized in that the extract of Hyunho color and plant extract using 10-95% ethanol as the extraction solvent.

In another aspect, the present invention provides a composition for external application for skin, characterized in that the extract is obtained by cold condensation at room temperature, heat filtration, and further obtained by concentrating the solvent under reduced pressure or lyophilization.

In addition, the present invention provides a topical skin composition, characterized in that the content of Hyunho colors and plant extracts is 0.001-30.0% by weight based on the total lyophilized weight of the external skin composition. When the content of the extract is less than 0.001% by weight, there is almost no skin improvement effect, and when the content of the extract is more than 30.0% by weight, the degree of increase in the effect of increasing the content is insignificant and thus it is not economical.

In addition, the present invention, the external composition for skin dermal calendula and plant, characterized in that selected from the formulation of the lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type and water-in-oil (W / O) type Provided is an external skin composition containing the extract as a main active ingredient.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, these Examples are only illustrative description of the present invention and the scope of the present invention is not limited to these Examples.

Example 1: Preparation of bleeding heart extract

After dipping, the dry bleeding heart was refluxed three times for 5 hours with an aqueous 95% (V / V) ethanol solution, and then cooled and filtered through Whatman # 5 filter paper. The filtered extract was concentrated under reduced pressure and lyophilized at 50 ° C or lower. A bleeding extract was prepared using one or more solvents selected from purified water, ethanol, butylene glycol, and propylene glycol so as to contain 0.001 to 30.0% by weight of the vacuum concentrate and the lyophilisate.

Example 2 Experiment of Measuring Antioxidant Effect Using NBT Method

In this example, to determine the antioxidant effect of the bleeding heart extract obtained in Example 1 as a comparative sample of other antioxidants, that is, green tea extract and vitamin E under laboratory conditions, antioxidant activity was measured using the NBT method.

In order to measure the antioxidant effect, the active oxygen produced by xanthine and xanthine oxidase is measured by the NBT method, and the effect of the test substance removing the active oxygen, that is, the active oxygen scavenging effect, is evaluated. Free radicals are produced by xanthine and xanthine oxidase. This active oxygen reacts with Nitro Blue Tetrazolium (NBT) to measure the active oxygen scavenging rate by measuring the blue color produced by this at a wavelength of 560 nm.

As a measuring method

0.05M Na ₂ CO ₃ ------------------------------------ 2.4ml

2.3mM xanthine solution ---------------------------------- 0.1ml

3.3mM EDTA Solution ------------------------------------ 0.1ml

4.BSA solution ---------------------------------------- 0.1ml

5.0.72 mM NBT solution ---------------------------------- 0.1ml

6.Xanthine Oxidase Solution ----------------------------- 0.1ml

7.6 mM CuCl ₂ solution ----------------------------------- 0.1ml

① Add 1,2,3,4,5 to Bayer's bottle and add 0.1ml of sample solution to it and leave at 25 ℃ for 10 minutes.

② Add 6 and stir quickly, and start incubation for 20 minutes at 25 ℃.

③ After that, add 7 to stop the reaction and measure the absorbance St at 560nm.

④ For the blank test, measure the absorbance Bt by using distilled water instead of the sample solution as above.

⑤ Also, the blank of the sample solution is operated in the same manner using distilled water instead of 6 to measure the absorbance Bo.

The result of the effect was calculated by Equation 1, the results are shown in Table 1.

% Inhibition = [1- (St-So) / (Bt-Bo)] X 100

St: absorbance at 560 nm after enzyme reaction of sample solution

Bt: absorbance at 560 nm after enzymatic reaction of blank test solution

So: absorbance at 560 nm before reaction without enzyme addition of sample solution

Bo: absorbance at 560 nm before reaction without enzyme in blank test solution

The results showed excellent antioxidant effect in all the samples tested as shown in Table 1, and it was confirmed that bleeding heart extract also had similar antioxidant power as green tea extract and vitamin E, which are widely used as antioxidants.

Sample Name Antioxidative effect(%) Dicentra D.W.Extract 85 Digestive Ethanol Extract 92 Green Tea Extract 94 vitamin 87

Example 3 Experimental Measurement of Human Fibroblast Activity

In this example, the cell line was Hs-68, which is a mouse-derived fibroblast, to confirm the effect of human fibroblast activity (Cell proliferation) of the alfalfa extract obtained in Example 1 and the number of 2 X 10 ⁵ cells per well in a 12-well plate. Dispense the cells and incubate for 24 hours. Each raw material is treated at a concentration that does not cause cytotoxicity, and after 24 hours of treatment with the sample, the medium is removed, and 500ul fresh medium and 60ul MTT solution are added to each well, followed by incubation in a 37 ° C CO ₂ incubator for 2 hours. Lysate the cells and transfer 100ul of each to a 96-well plate. Absorbance was measured at 565 nm of ELISA reader.

The degree of cell activity was calculated by Equation 2 below. Based on the number of cells that did not process anything. The results are shown in Table 2, it can be seen that the bleeding heart extract is relatively excellent in the cell activity effect.

Cell activity rate (%) = (cell number in sample treatment-reference cell number) / reference cell number X 100

Activity rate below 10 10-20% 20-50% 50% or more Active effect none usually Great Very good

The results are shown in Table 3.

% Active Dicentra Extract 0.1% 137 Dicentra Extract 0.01% 112 Control 100

Example 4: in vitro MMP-1 inhibition assay

Substrate metalloproteinase (MMP-1) inhibitory activity was measured in vitro in a screening type biochemical model based on the use of purified collagenase and its substrate, collagen and gelatin in contact with fluorescein. (EnzChek ™ gelatinase / collagenase kit, molculaprobe). Collagenase purified from Clostridium histiocom was supplied in the EnzChek ™ gelatinase / collagenase kit. This enzyme has dual action on collagen types I and IV, gelatin. A reaction buffer consisting of DQ-collagen purified from porcine skin and conjugated to fluorescein with 0.05M Tris-HCl, 0.15M NaCl, 5mM CaCl ₂ and 0.2mM sodium azide (pH 7.6) was EnzChek (TM) gelatinase. Collagenase kit (Molcula Probes) was used. Dicentra extract was dissolved in the reaction buffer. It is 4; 2; 0.4; Tested at 0.2; 0.04% (/ v). Dilutions of the test extracts were incubated with 25 ug / ml of DQ-collagen and 0.1 U / ml of collagenase for 15 minutes, 45 minutes, and 120 minutes at room temperature.

In each experimental condition, the control corresponding to the collagenase and DQ-collagen mixture was also incubated in the same manner. Also in each experimental condition a sample called Blank, hereinafter 'enzyme free blank', was incubated in the presence of DQ-collagen and in the absence of collagenase. Each experiment was performed three times.

After 15, 45 and 120 minutes, the signal corresponding to the degradation of DQ-collagen was measured with a fluorometer (excitation: 485 nm, emission 505 nm). The fluorescence value of each sample was measured based on the 'enzyme free blank' fluorescence value. Results are expressed as percent variance for fluorescence units per sample and control. The results are shown in pluorescein units / sample.

All tests used 1,10-phenanthroline, a nonspecific metal chelator inhibitor, as a control inhibitor. At 0.1 U / ml collagenase the inhibitor is suitably at a concentration of 0.2 mM.

In conclusion, the bleeding heart extract tested at 0.04 to 4% (v / v) under selected experimental conditions retained dose dependent anticollagenase / gelatinase activity.

The results are shown in Tables 4 and 5, and the digestive ethanol extract inhibited 70% of the collagen degrading activity of Clostridium collagenase at 0.04%, which was similar to the inhibitory effect of 2mM 1,10-phenanthroline. Digestive hydrothermal extract inhibited 75% of collagen degrading activity at 0.2% treatment, which was superior to the inhibitory effect of 2mM 1,10-phenanthroline.

0.1 U collagenase 2 mM 1,10-phenanthroline Digestive Ethanol Extract 4 2 0.4 0.2 0.04 Fluorescence 220.1 62.17 13 41 69 85 98 Enzyme activity 100% 28 5 18 31 38 44 Inhibition rate - 72 95 82 69 62 56

0.1 U collagenase 2 mM 1,10-phenanthroline Dicentra D.W. extract 4 2 0.4 0.2 0.04 Fluorescence 220.1 62.17 25.448 78 82 109 112 Enzyme activity 100% 28 11.4 25 37 49 50.8 Inhibition rate - 72 88.6 75 63 51 49.2

Example 5: in vitro MMP-2 inhibition assay

Matrix metalloproteinase (MMP-2), gelatinase inhibitory activity was measured in vitro in a screening type biochemical model, where MMP-2 or gelatinase A was a merosa that degrades specific components of the extracellular matrix. As proteases it degrades gelatin (modified collagen), collagen I, IV, VIII, and VIII, and fibronectin, laminin and elastin. MMP-2 purified from human fibroids was obtained from Colbiochem and gelatin in contact with its substrate fluorescein (EnzChek ™ gelatinase / collagenase kit, Mocula Probes).

MMP-2 inhibitory activity of bleeding heart extract was tested in the same manner as the MMP-1 experiment. Dilutions of the test extracts were incubated with 25 ug / ml DQ-gelatin and 0.1 U / ml gelatinase for 15 minutes, 45 minutes, and 120 minutes at room temperature.

The results are shown in Table 6 and Table 7. Digestive ethanol extract inhibited 82% of the gelatin degrading activity of gelatinase purified from human fibroids when treated with 0.04%. % Was inhibited, similar to the inhibitory effect of 2 mM 1,10-phenanthroline.

0.1 U gelatine 2 mM 1,10-phenanthroline Digestive Ethanol Extract 4 2 0.4 0.2 0.04 Fluorescence 417.4 74.03 24 53 77 102 253 Enzyme activity 100% 17.7 5.7 12.7 18 24 60 Inhibition rate - 82.2 94.2 87.3 82 76 40

0.1 U collagenase 2 mM 1,10-phenanthroline Dicentra D.W. extract 4 2 0.4 0.2 0.04 Fluorescence 220.1 62.17 25.448 78 82 109 112 Enzyme activity 100% 28 11.4 25 37 49 50.8 Inhibition rate - 72 88.6 75 63 51 49.2

Example 6: Evaluation of the inhibition of MMP-1 and MMP-2 expression by bleeding heart extract after UV irradiation

This Example was subjected to ELISA to measure the concentration of MMP-1 and MMP-2 after UV irradiation and sample addition of the bleeding heart extract obtained in Example 1.

Human dermal fibroblasts are irradiated with UVA at an energy of 5 J / cm 2 using a UV chamber. UV irradiation dose and culture time were established through preliminary experiments to maximize the expression of MMP in fibroblasts. Negative controls were wrapped in tinfoil and maintained at the same time in the environment of UVA. UVA emission was measured using a UV radiometer. The cells during the UVA irradiation are intact in the previously dispensed medium and irradiated with UVA and then exchanged with the medium containing the sample for 24 hours of incubation, and then the medium is recovered and coated in 96-well. The primary antibody (MMP-1 (Ab-5) monoclonal antibody and MMP-2 (Ab-3) monoclonal antibody) is treated and reacted at 37 ° C. for 60 minutes. After reacting the secondary antibody anti-mouse IgG (whole mouse, alkaline phosphatase conjugated) for about 60 minutes, the alkaline phosphatase substrate solution (1mg / mlρ-nitrophenyl phosphate in diethanolamine buffer solution) was reacted at room temperature for 30 minutes and then the microplate reader was reacted. Measure the absorbance at 405 nm. As a control, the one without addition of the sample is used.

In case of UV-induced expression, the bleeding ethanol extract showed more than 90% inhibition rate compared to the control group without MMP-1 and MMP-2 expression, which is similar to the inhibition rate of retinol used as a control.

Test group % Inhibition of MMP-1 expression % Inhibition of MMP-2 expression Control - - Dicentra hydrothermal extract 0.1% 58 83 Digestive Ethanol Extract 0.1% 95 91 Retinol 96 90

Examples 7 to 9 and Comparative Example 1

In this example, the cosmetics containing the bleeding heart extract obtained in Example 1 were prepared, and the skin wrinkle improvement and skin beauty effects of humans were evaluated by comparative experiments with Comparative Example 1.

The cosmetics used in the comparative experiments are in the form of a cream, the composition of which is shown in Table 9. First, the phase b) recorded in Table 9 is heated and stored at 70 ° C. To this, a) phase is added, and after pre-emulsification, it is emulsified uniformly with a homomixer, and then cooled slowly to prepare a cream (Examples 7 to 9 and Comparative Example 1). For 20 experimenters (20-35 year old female), the creams prepared in Examples 7 to 9 were applied to the right side of the face and the creams prepared in Comparative Example 1 were applied twice a day for 3 consecutive months to the left side of the face. It was.

After completion of the experiment, the skin wrinkle reduction effect is collected before and after using the product for 3 months by using a silicone resin copy (replica) around the face of the eye, and using the skin fine wrinkle device and skin image analyzer, the condition of the eye wrinkles and the color difference meter are used. Compare skin tone improvement.

Raw material Example Comparative example 7 8 9 end Stearyl alcohol 8 8 8 8 Stearic acid 2 2 2 2 Stearin cholesterol 2 2 2 2 Squalene 4 4 4 4 2-octyldodecyl alcohol 6 6 6 6 Polyoxyethylene (25 mol addition) alcohol ester 3 3 3 3 Glyceryl monostearic acid ester 2 2 2 2 I Dicentra Extract 0.1 One 5 - Propylene glycol 5 5 5 5 Purified water Quantity Quantity Quantity Quantity

Note) Unit: Weight%

Item % Reduction Comparative example Example 9 Deepest crease depth (RZ, mm) 1.54 12.7 Average wrinkle depth (RA, mm) 13.6 21.3

Table 10 compares the average eye wrinkle depth and wrinkle reduction rate of the experimenter using the cream prepared in Example 9 with the experimenter using the cream of Comparative Example 1. As shown in Table 10, it can be seen that the skin wrinkle improvement effect is excellent in the skin around the face of the experimenter who applied the cream containing bleeding heart extract.

Other examples are shown below. That is, the lotion, milky lotion and essence containing the bleeding heart extract obtained in Example 1 were prepared in Examples 10 to 12. The lotion, milky lotion and essence containing the bleeding extract showed excellent effects on skin improvement such as antioxidant effect, collagen synthesis promoting effect, and skin wrinkle improvement.

Example 10 Preparation of Lotion Containing the Digestive Extract Extracted in Example 1

To 8 g of 95% ethanol, 0.05 g of polypyrrolidone, 0.1 g of oleyl alcohol, 0.2 g of polyoxyethylene monooleate, 0.2 g of fragrance, 0.1 g of paraoxybenzoic acid methyl ester, a small amount of antioxidant, and a small amount of pigment are dissolved. . 0.05 g of the bleeding heart extract obtained in Example 1 and 5 g of glycerin were dissolved in 85.33 g of purified water, and then the mixed solution was added and stirred to obtain a lotion having a skin improvement effect.

Example 11: Preparation of milky milk containing bleeding heart extract obtained in Example 1

1.2 g of cetyl alcohol, 10 g of squalane, 2 g of petrolatum, 0.2 g of paraoxybenzoic acid ethyl ester, 1 g of glycerin monoesteralide, 1 g of polyoxyethylene (20 mole added) monooleate and 0.1 g of fragrance were mixed and dissolved at 70 ° C. , 0.5 g of the bleeding heart extract obtained in Example 1, 5 g of dipropylene glycol, 2 g of polyethylene glycol-1500, 0.2 g of triethanolamine, and 76.2 g of purified water were dissolved by heating to 75 ° C. Both mixtures were emulsified and cooled to obtain an emulsion having an oil-in-water (O / W) type skin improvement effect.

Example 12 Preparation of a Cosmetic Solution Containing a Dicentra Extract Obtained in Example 1

5 g of 95% ethyl alcohol, 1.2 g of polyoxyethylene sorbitan monooleate, 0.3 g of kitulose, 0.2 g of sodium hyaluronate, 0.2 g of vitamin E-acetate, 0.2 g of sodium licorice, 0.1 g of paraoxybenzoic acid ethyl ester, 1 g of the bleeding heart extract obtained in Example 1 and an appropriate amount of pigment were mixed to obtain a essence having a skin improvement effect.

As described above, the extract of the cortex and plant extract according to the present invention specifically inhibits the activity of substrate metalloproteinase (MMP), specifically collagenase (MMP-1) and gelatinase (MMP-2), as enzyme inhibitors. Cosmetic compositions such as lotion, cream, milky lotion, and packs containing such bleeding extracts in human dermal dermal fibroblasts by UV irradiation have anti-aging effects by MMP enzyme inhibitory effect and MMP expression inhibitory effect induced by UV light. It can be seen.

Claims (7)

A skin external preparation composition containing an extract of an Fumaiaceae plant as a main active ingredient. The composition for external application for skin according to claim 1, wherein the Fumaiaceae plant is dicentra spectabisis. The method according to claim 1 or 2, characterized in that the extraction is carried out using one or more solvents selected from purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane and hexane as the extraction solvent, A composition for external application of skin, comprising an extract of the genus Cortex and a plant as its main active ingredient. According to claim 3, 10-95% ethanol is used as the extraction solvent, characterized in that the skin external composition containing the extract of the genus horticulture as the main active ingredient. According to claim 1 or 2, wherein the extract is a liquid obtained by cooling, heating and filtration at room temperature, further characterized in that the solvent obtained by concentrated under reduced pressure or lyophilization, containing an extract of an orange and plant as the main active ingredient Skin external preparation composition. According to claim 1 or 2, wherein the content of Hyunho color and plant extract is 0.001-30.0% by weight based on the freeze-dried weight of the total cosmetic composition, the external skin preparation containing Hyunho color and plant extract as the main active ingredient Composition. The cosmetic composition of claim 1 or 2, wherein the cosmetic composition is selected from a lotion, gel, water-soluble liquid, cream, essence, oil-in-water (O / W) type, and water-in-oil (W / O) type. To the external composition for skin containing the genus quince and plant extract as the main active ingredient.