KR100630410B1 - Topical compositions of herbal extracts for prevention and cure of acne - Google Patents

Abstract

An externally applied composition for treating acne is provided to effectively inhibit proliferation of Propionibacterium acnes by comprising at least one methanol extract of aloe, hemp seed, Chelidonii Herba and Corydalis turtschaninovii extract. The external composition for preventing and treating acne comprises 0.1-5 wt.% of at least one herb extract selected from the group consisting of Chinemys reevesii Gray extract, aloe extract, hemp seed extract, Chelidonii Herba extract and Corydalis turtschaninovii extract.

Description

Topical compositions for the prevention and treatment of acne containing herbal extracts {topical compositions of herbal extracts for prevention and cure of acne}

Figure 1 shows the activity of P.acnes for each concentration of ear plate extract of the present invention.

Figure 2 shows the activity of P. acnes for each concentration of the extract of the present invention.

Figure 3 shows the activity of P. acnes for each concentration of the extract as soon as the present invention.

Figure 4 shows the activity of P. acnes for each concentration of the extract of white buckwheat extract of the present invention.

Figure 5 shows the activity of P. acnes for each concentration of corydalis extract of the present invention.

Figure 6a is a state immediately after applying the herbal extract of the present invention to the inner skin of the arm.

Figure 6b is after 48 hours after applying the herbal extract of the present invention to the inner skin of the arm.

Antimicrobial agents used as external preparations for the prevention and treatment of acne include erythromycin, clindamycin, and benzoyl peroxide. However, the antimicrobial agents used to treat the acne have problems such as generation of resistant bacteria, non-selective sterilization or bacteriostatic action. Therefore, there is a need to find an effective substance that can replace these existing antimicrobial agents.

In the process of selecting natural substitutes, the inventors have found that the ear extract, presbyopia, mazain, white oyster and methanol extract of Hyunho color effectively inhibit P. acnes proliferation. The composition containing the herbal extract of the present invention may provide an effective means for preventing or treating acne, which is problematic for many people, by replacing the existing antimicrobial agents used in the treatment of acne.

The present invention effectively prevents the growth of P. acnes by preventing the acne outbreaks that exhibit P. acnes proliferation inhibitory ability that can be used for the prevention and treatment of acne, presence, mage, white oysters, a composition containing methanol extract of scallop And to provide as a therapeutic.

BBL Gas Pack anaerobic culture vessel was used to measure the growth inhibitory effect of P. acnes . The test strain was Propionibacterium acnes KCTC3314.

The herbal medicines used for the measurement of growth inhibitory effect are ear plate, rosacea, mazain, white oyster, and navy color. 50 g of each herb was extracted with 500 ml of methanol for 3 hours by reflux cooling to remove the solvent, and the residue was used as a sample for the following experiment. It was.

To test the growth inhibitory effect of the acne causative bacteria of the herbal extracts were suspended 50mg of the herbal extracts in 1ml of distilled water was used as a sample solution.

The medium used for culturing P. acnes was Actinomyces broth agar medium.

The microorganisms were cultured in a 37 ° C. incubator.

Example 1 Inhibitory Effect of Ear Disc Extract on P. acnes

50 mg of ear plate extract is suspended in 1 ml of distilled water and centrifuged. The supernatant is mixed with 4 ml of Actinomyces broth agar medium, and the plated medium is made. The cultured P. acnes microorganisms are suspended in sterile distilled water and A ₆₀₀ is 0.1 3 µl inoculation was carried out using Pipetman and cultured to observe the growth of the bacteria. Experimental results were as shown in FIG.

Example 2 Growth Inhibition Effects of P. acnes Extracts from

By suspending the classis extract 50mg in 1ml of distilled water, and centrifugation the supernatant was mixed with 60, 40, 20 ㎕ respectively in Actinomyces broth agar medium 4ml making the plate medium were suspended in sterile distilled water in cultured P. acnes microorganisms yi A ₆₀₀ 0.1 3 µl inoculation was carried out using Pipetman and cultured to observe the growth of the bacteria. Experimental results were as shown in FIG.

Example 3 Growth Inhibition Effect of P. acnes of Mazarine Extracts

Suspended 50 mg of the extract in 1 ml of distilled water and centrifuged to mix the supernatant 60, 40, 20 μl each in 4 ml of Actinomyces broth agar medium to prepare a plate medium and cultured P. acnes microorganisms suspended in sterile distilled water to A ₆₀₀ 0.1 μl was used to inoculate 3 μl using Pipetman and cultured to observe the growth of the bacteria. Experimental results were the same as in FIG. 3.

Example 4 Growth Inhibition Effect of P. acnes Extract

50 mg of Baekgulchae extract was suspended in 1 ml of distilled water and centrifuged. The supernatant was mixed with 4, 60 ml of Actinomyces broth agar medium, and the plated medium was made. The cultured P. acnes microorganisms were suspended in sterile distilled water to give A ₆₀₀ . 0.1 μl was used to inoculate 3 μl using Pipetman and cultured to observe the growth of the bacteria. Experimental results were as shown in FIG.

Example 5 Growth Inhibition Effect of Corydalis Root Extract on P. acnes

By suspending the Corydalis extract 50mg in 1ml of distilled water, and centrifugation the supernatant was mixed with 60, 40, 20 ㎕ respectively in Actinomyces broth agar medium 4ml making the plate medium were suspended in sterile distilled water in cultured P. acnes microorganisms yi A ₆₀₀ 0.1 3 µl inoculation was carried out using Pipetman and cultured to observe the growth of the bacteria. Experimental results were as shown in FIG.

As can be seen from the above examples, ear plate, sashimi, madin, baekryechae, hyacinth extract can be found to effectively inhibit P. acnes , the cause of acne-producing bacteria at low concentrations, so that they can be used for the prevention and treatment of acne. Can be.

For reference, the concentration of antimicrobial agents used in prophylactic treatment of acne in the clinic is 1.5-3.0% for erythromycin, 1% for clindamycin, and 5% for benzoyl peroxide.

Therefore, the composition of the present invention containing one or more of the ear plate, presbyopia, mazain, baekryechae, Hyunho color extract of the present invention can inhibit the growth of acne-causing bacteria by substituting conventional antimicrobial agents used in conventional acne treatment It is possible to provide an effective acne prevention and treatment agent while minimizing the skin effects resulting from the use of conventional antibiotics.

Test Example 1

In order to test the skin applicability of ear plate, presbyopia, mazain, baekryechae, and calendula extract, the skin safety test was performed to determine the effect of contact dermatitis at concentrations of 0.01% to 5%. 50 μl of the diameter of the suspension suspension was imposed on the inner side of the arm (FIG. 6A), and after 48 hours, redness was examined (FIG. 6B) to examine irritation in 20 people. As shown in Table 1, there was no possibility of causing contact dermatitis.

Extract concentration Contact rate of dermatitis (%) Earboard Mazain White oyster Corydalis craftiness 0.01% 0 0 0 0 0 0.1% 0 0 0 0 0 0.5% 0 0 0 0 0 1.0% 0 0 0 0 0 5.0% 0 0 0 0 0

The formulation of the acne preventive and therapeutic agent of the present invention may be any formulation that can be applied externally to the skin such as cream, lotion, solution, etc., and may be provided by a conventional formulation method.

Formulation Example 1

An example of a lotion formulation as an example of the formulation is as follows (unit weight percent).

Crude Extract 0.1%, Stearic Acid 0.5%, Cetanol 1.2%, Glyceryl Trioctanoate 1.5%, Squalane 2.0%, Propyl Paraben 0.1%, Butyl Paraben 0.05%, Dimethicone 0.3%, Solbitan Seskiol 0.2%, Tuin 60 0.3%, Glycerin 7.0%, Imidazolidinyl urea 0.15%, Triethanolamine 0.15%, Carbomer 0.1%, Distilled water appropriate amount.

Test Example 2

The results of the evaluation of the efficacy evaluation on the prevention and treatment of acne outbreak for 10 adults of Formulation Example 1 are as follows. In the prevention and treatment of acne, a lotion preparation containing 0.1% of the extract was applied to the skin for 10 days after washing twice a day in the morning and evening, as shown in Table 2 below. It showed an effect.

A review item In effect no effect Test score Prevention of Acne 7 people 3 people 70% Acne improvement 8 people 2 people 80%

Therefore, the preferred concentrations of ear extract, rosacea, mazain, baekryechae, Hyunho-red extract as external preparations to be applied to the present invention are expected to be effective in preventing and treating acne. In some cases, up to 10% by weight may be used.

If it is used at 0.1% by weight or less, it is difficult to expect the desirable effects of the present invention. If it is used at 10% by weight or more, further effects cannot be expected, which is not preferable.

For the prevention and treatment of acne, it is preferable to apply the external preparation to the skin after washing twice a day in the morning and evening.

It can contribute to improving the quality of life of humans by providing natural acne prevention and treatments containing ear extract, presbyopia, mazain, white oyster, and corydalis extract at low concentrations that inhibit the growth of P. acnes bacteria.

Claims (2)

An external preparation composition for the prevention and treatment of acne containing 0.1 wt% to 5 wt% of at least one selected from ear plate extract, sediment extract, mother extract, white oyster extract, and corydalis extract. The external preparation composition according to claim 1, wherein the acne prevention and treatment effect is due to inhibition of propagation of Propionibacterium acnes ( P. acnes ).

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