KR20020078134A - Advanced Cosmetics Comprising Tannin as an Active Ingradient - Google Patents
Advanced Cosmetics Comprising Tannin as an Active Ingradient Download PDFInfo
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Abstract
Description
본 발명은 탄닌을 유효성분으로 함유하는 기능성 화장품에 관한 것이다. 좀 더 구체적으로, 본 발명은 항산화제로 알려져 있는 탄닌을 유효성분으로 함유하는 기능성 화장품에 관한 것이다.The present invention relates to a functional cosmetic containing tannin as an active ingredient. More specifically, the present invention relates to a functional cosmetic containing tannin, known as an antioxidant, as an active ingredient.
동물의 피부는 외부환경으로 부터 생체를 보호하는 1차적인 보호막으로서, 외부환경의 영향을 여과없이 받게 된다. 이러한 영향 중, 대기 오염물질 또는 자외선 등의 유해요인에 의한 손상이 두드러지는데, 자외선의 경우, 지구에 도달하는 빛의 0.5%에 해당하는 극히 적은 양이지만, 홍반, 색소침착, 주름 및 대부분의 피부암을 유발시키는 요소로서 작용한다고 알려져 있다. 최근에는 생태계의 불균형 및 불안정화로 인해서, 대기권의 오존층이 점차 얇아짐에 따라서 지표에 도달하는 자외선의 양은 점차 늘어가고 있으며, 오존층이 1% 감소함에 따라서 악성흑색종이 0.6%씩 증가한다고 보고되고 있는 실정이다. 따라서, 자외선 및 기타 영향에 의한 피부손상을 치료하려는 다각적인 노력이 지속되고 있다.The skin of an animal is the primary protective film that protects the living body from the external environment, and receives the effects of the external environment without filtering. Among these effects, damage caused by harmful pollutants such as air pollutants or ultraviolet rays is prominent. In the case of ultraviolet rays, an extremely small amount corresponding to 0.5% of the light reaching the earth, erythema, pigmentation, wrinkles and most skin cancers It is known to act as an inducing factor. Recently, due to the imbalance and destabilization of the ecosystem, the amount of ultraviolet rays reaching the surface is gradually increasing as the ozone layer in the atmosphere becomes thinner, and malignant melanoma increases by 0.6% as the ozone layer decreases by 1%. to be. Accordingly, various efforts have been made to treat skin damage caused by ultraviolet rays and other effects.
근래에 와서, 녹차추출물이 피부손상 및 피부질환의 치료에 효과적이라는 것이 밝혀져 있고, 이의 유효성분이 카테킨 화합물이라고 보고되어 있다. 이에, 카테킨 화합물을 이용하여 피부손상을 치료하려는 노력이 계속되고 있으나, 녹차에서 추출되는 카테킨 화합물은 많은 화합물의 혼합물인 관계로 제품개발에 큰 어려움을 겪고 있는 실정이다.In recent years, it has been found that green tea extract is effective for the treatment of skin damage and skin diseases, and its active ingredient is reported to be a catechin compound. Thus, efforts to cure skin damage using catechin compounds continue, but catechin compounds extracted from green tea are a mixture of many compounds, and thus are experiencing great difficulties in product development.
따라서, 피부손상 및 피부질환에 효과적인 단일성분을 개발하여야 할 필요성이 끊임없이 대두되었다.Therefore, there is a constant need to develop effective single components for skin damage and skin diseases.
이에, 본 발명자들은 카테킨 화합물 중 피부손상 및 피부질환에 효과적인 성분을 찾아내고자 예의 연구노력한 결과, 녹차에서 추출된 카테킨 화합물 중, 탄닌이 주름의 치료 및 자외선으로 인한 홍반 및 색소침착의 생성억제에 특이적인 효과를 나타내며, 탄닌을 유효성분으로 함유하는 기능성 화장품을 피부에 처리하면, 이러한 효과가 극대화됨을 확인하고, 본 발명을 완성하게 되었다.Accordingly, the present inventors have made intensive studies to find an effective ingredient for skin damage and skin diseases among catechin compounds. Among the catechin compounds extracted from green tea, tannins are specific for the treatment of wrinkles and the suppression of erythema and pigmentation due to ultraviolet rays. When the functional cosmetics containing tannins as an active ingredient are treated on the skin, it is confirmed that such effects are maximized and the present invention has been completed.
결국, 본 발명의 주된 목적은 탄닌을 유효성분으로 함유하는 기능성 화장품을 제공하는 것이다.After all, the main object of the present invention is to provide a functional cosmetic containing tannin as an active ingredient.
도 1은 탄닌의 피부주름치료 효과를 나타내는 그래프이다.1 is a graph showing the skin wrinkle treatment effect of tannins.
도 2는 탄닌의 홍반형성억제 효과를 나타내는 그래프이다.2 is a graph showing the erythema suppression effect of tannins.
도 3은 탄닌의 피부경화억제 효과를 나타내는 그래프이다.3 is a graph showing the skin hardening inhibitory effect of tannins.
본 발명의 탄닌을 유효성분으로 함유하는 기능성 화장품은 1 내지 10%(w/v)의 탄닌과 화장료를 포함한다.Functional cosmetics containing tannin of the present invention as an active ingredient includes 1 to 10% (w / v) of tannin and cosmetics.
탄닌(tannin)은 주로 식물의 잎이나 줄기, 뿌리, 열매 등에 널리 분포되어 있는 폴리페놀계 물질이며, 특히 감, 밤, 녹차 그리고 덜 익은 과일류에 많이 함유되어 있다. 현재까지 알려진 탄닌은 혈관에 쌓여 있는 콜레스테롤 지방산이나 노폐물 등을 용해시켜 주는 작용을 하여, 뇌졸중, 관상동맥 혈전증, 협심증, 심근경색과 같은 혈관계 질환의 예방과 치료에 효과적임이 보고되어 있을 뿐만 아니라, 유해산소를 제거하는 항산화제, 항암제 및 돌연변이 유발 물질의 활성을 감소시키는 물질로서 알려져 있다. 근래에는, 세포에 대한 산화성 손상(oxidant damage)으로부터 세포를 보호하는데 중요한 역할을 하고, 많은 종류의 곰팡이, 효모, 세균 및 바이러스의 생육을 억제시키는 효과가 보고되고 있다.Tannin is a polyphenolic substance widely distributed in the leaves, stems, roots, and fruits of plants, and is particularly found in persimmons, chestnuts, green tea, and less ripe fruits. Tannins, known to date, dissolve cholesterol fatty acids and waste products accumulated in blood vessels, and have been reported to be effective in preventing and treating vascular diseases such as stroke, coronary thrombosis, angina pectoris, and myocardial infarction. It is known as a substance that reduces the activity of antioxidants, anticancer agents and mutagenic substances which remove oxygen. In recent years, it has been reported to play an important role in protecting cells from oxidant damage to cells and to inhibit the growth of many types of fungi, yeast, bacteria and viruses.
한편, 피부와 관련하여 탄닌의 효과에 대한 연구는 미흡한 실정이지만, 지금까지의 연구결과로는 탄닌이 일차적으로 피부보호막을 형성함으로써 물리적이고 화학적인 요소로 인해 발생되는 피부암을 억제시킬 수 있다고 알려져 있는데, 전임상실험 연구를 통해 밝혀진 탄닌의 피부암 억제 기작은, 유리라디칼의 생성을 억제하고 발암물질로 인한 세포의 종양화유도를 억제함으로써 피부암 생성을 억제시킨다고 보고된 바 있다.On the other hand, studies on the effects of tannins on the skin have been insufficient. However, previous studies have shown that tannins can form skin barriers to prevent skin cancer caused by physical and chemical factors. The mechanism of inhibiting skin cancer of tannins revealed by preclinical studies has been reported to inhibit skin cancer production by inhibiting the production of free radicals and inhibiting the induction of tumor cells by carcinogens.
본 발명의 탄닌을 유효성분으로 함유하는 기능성 화장품은 통상적으로 사용되는 화장료를 함유할 수 있는데, 예를 들면 수용성 스킨제제화를 위하여 글리세롤, 프로필렌글리콜, 1,3-부틸렌글리콜, 솔비톨, 폴리에틸렌글리콜, 카르복시비닐폴리머, 잔탄검, 카르복시메틸셀룰로오스, 하이드록시에틸셀룰로오스, 하이드록시메틸셀룰로오스, 로커스트빈검, 알란토인, 카라기난 등을 첨가할 수 있으며; 점도와 경도조절제로 밀납, 파라핀 왁스, 스테아릴알콜, 카르나우바 왁스, 칸데릴라 왁스 및 칼슘스테아레이트, 알루미늄스테아레이트, 아연스테아레이트, 위치하젤(witchhazel) 등을 사용할 수 있고; 자외선 흡수제로 부틸메톡시디벤조일메탄, 옥틸메톡시신나메이트 등을 사용할 수 있으며; 안료로는 이산화티탄, 미립자 이산화디탄, 카올린, 나이론 파우다, 탈크, 세리사이트, 마이카, 폴리메틸메타크릴레이트 등의 체질 안료와 황색산화철, 흑색산화철, 적색산화철, 울트라마린, 산화크롬, 수산화크롬 등의 착색안료를 사용할 수 있고; 보습제로 1,3-부틸렌글리콜, 농글리세린, 에틸렌글리콜 등과 키틴, 키토산, 히아론산, 하이알루로닌산, 젖산, 글리콜산 등의 천연보습 물질들을 이용할 수 있으며; 방부제로 파라옥시안식향산 에스테르류, 이미다졸리디닐우레아 등을 사용할 수 있을 뿐만 아니라, 상기한 성분들을 제품특성에 따라 1종 또는 2종이상 혼용 배합할 수도 있다.Functional cosmetics containing tannin of the present invention as an active ingredient may contain cosmetics commonly used, for example, glycerol, propylene glycol, 1,3-butylene glycol, sorbitol, polyethylene glycol, Carboxyvinyl polymer, xanthan gum, carboxymethyl cellulose, hydroxyethyl cellulose, hydroxymethyl cellulose, locust bean gum, allantoin, carrageenan and the like can be added; Beeswax, paraffin wax, stearyl alcohol, carnauba wax, candelilla wax and calcium stearate, aluminum stearate, zinc stearate, witchhazel and the like as viscosity and hardness modifiers; Butylmethoxydibenzoylmethane, octylmethoxycinnamate and the like can be used as the ultraviolet absorber; As pigments, extender pigments such as titanium dioxide, finely divided titanium dioxide, kaolin, nylon powder, talc, sericite, mica, polymethyl methacrylate, yellow iron oxide, black iron oxide, red iron oxide, ultramarine, chromium oxide, chromium hydroxide, etc. Can be used for coloring pigments; As moisturizers, natural moisturizing substances such as 1,3-butylene glycol, concentrated glycerin, ethylene glycol and the like, chitin, chitosan, hyaluronic acid, hyaluronic acid, lactic acid, glycolic acid, etc. may be used; As the preservative, not only paraoxybenzoic acid esters, imidazolidinyl urea and the like can be used, but also the above-described components may be mixed in one kind or two or more kinds according to product characteristics.
또한, 본 발명의 기능성 화장품은 메이컵 화장료의 제형, 크림, 로션, 스킨, 아스트리젠트, 화장수, 에센스 등의 제형으로 제조될 수 있으며, 특히, 10 내지 30%(v/v)의 화장료 및 70 내지 90%(v/v)의 정제수를 포함하는 수용성 화장품의 제형으로 제조함이 가장 바람직하다.In addition, the functional cosmetics of the present invention may be prepared in the formulation of makeup cosmetics, creams, lotions, skins, astringents, lotions, essences, etc., in particular, 10 to 30% (v / v) of cosmetics and 70 Most preferably, the formulation is prepared in the form of a water-soluble cosmetic containing from 90% (v / v) purified water.
본 발명의 기능성 화장품은 함유된 탄닌의 효과로 인하여 자외선에 의하여 유발된 피부주름, 홍반 및 색소침착을 치료 또는 억제하는 효과를 나타내므로, 피부손상의 예방 및 치료에 널리 활용될 수 있을 것이다.Functional cosmetics of the present invention exhibits the effect of treating or inhibiting skin wrinkles, erythema and pigmentation caused by ultraviolet rays due to the effect of the tannin contained, it can be widely used for the prevention and treatment of skin damage.
이하, 실시예를 통하여 본 발명을 보다 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those of ordinary skill in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .
실시예 1: 기능성 화장품의 제조 Example 1 Preparation of Functional Cosmetics
다음과 같은 3가지의 탄닌을 포함하는 기능성 화장품을 유연화장수 형태로 제조하였다.Functional cosmetics containing the following three tannins were prepared in a flexible cosmetic form.
정제수 80.1%(v/v), 1,3-부틸렌글리콜 8.5%(v/v), 글리세린 10.0%(v/v), 카르복시비닐폴리머 0.2%(v/v), 카라기난 0.075%(v/v), 알란토인 0.1%(v/v), 위치하젤 1.0%(v/v) 및 하이알루로닌산 0.025%(v/v)의 혼합물에 탄닌 2.5%(w/v)를 용해시킨 화장품 A를 제조하였다.Purified water 80.1% (v / v), 1,3-butylene glycol 8.5% (v / v), glycerin 10.0% (v / v), carboxyvinyl polymer 0.2% (v / v), carrageenan 0.075% (v / v) v) cosmetic A containing 2.5% (w / v) of tannin dissolved in a mixture of allantoin 0.1% (v / v), witch hazel 1.0% (v / v) and hyaluronic acid 0.025% (v / v). Prepared.
정제수 84.87%(v/v), 1,3-부틸렌글리콜 7.5%(v/v), 프로필렌글리콜5.0%(v/v), 카르복시비닐폴리머 0.2%(v/v), 카리기난 0.08%(v/v), 알란토인 0.1%(v/v), 위치하젤 1.0%(v/v) 및 젖산 1.5%(v/v)의 혼합물에 탄닌 1.25%(w/v)를 용해시킨 화장품 B를 제조하였다.Purified water 84.87% (v / v), 1,3-butylene glycol 7.5% (v / v), propylene glycol 5.0% (v / v), carboxyvinyl polymer 0.2% (v / v), carrageenan 0.08% ( v / v), Allantoin 0.1% (v / v), Witch Hazel 1.0% (v / v) and lactic acid 1.5% (v / v) dissolved in tannin 1.25% (w / v) to prepare a cosmetic B It was.
정제수 88.1075%(v/v), 1,3-부틸렌글리콜 8.0%(v/v), 프로필렌글리콜 2.0%(v/v), 카르복시비닐폴리머 0.2%(v/v), 카리기난 0.08%(v/v), 알란토인 0.1%(v/v), 위치하젤 1.0%(v/v), 하이알루로닌산 0.0125%(v/v) 및 글리콜산 0.5%(v/v)의 혼합물에 탄닌 2%(w/v)를 용해시킨 화장품 C를 제조하였다.Purified water 88.1075% (v / v), 1,3-butylene glycol 8.0% (v / v), propylene glycol 2.0% (v / v), carboxyvinyl polymer 0.2% (v / v), carrageenan 0.08% ( v / v), allantoin 0.1% (v / v), witch hazel 1.0% (v / v), tannin 2 in a mixture of hyaluronic acid 0.0125% (v / v) and glycolic acid 0.5% (v / v) Cosmetic C prepared by dissolving% (w / v) was prepared.
실시예 2: 기능성 화장품의 주름치료효과 Example 2 Wrinkle Treatment Effect of Functional Cosmetics
실시예 1에서 제조된 기능성 화장품의 주름치료효과를 다음과 같이 알아보았다: 먼저, 6주령된 누드마우스(albino SKH-1 hairless mice, 대한실험동물 실험사, 대한민국)를 자외선램프가 구비된 자외선 상자에 넣고, 150mJ/cm2의 조사량으로 1일 1회, 6주동안 자외선을 조사하였다. 이어, 실시예 1에서 제조된 화장품 A를 200㎕/㎠의 양으로 누드마우스에 처리한 실험군과, 탄닌성분이 없는 것을 제외하고는 화장품 A와 동일한 조성비를 가지는 화장품을 200㎕/㎠의 양으로 누드마우스에 처리한 대조군을 비교하고, 그 결과를 피부복제 키트(skin replica kit, C+K, 독일)를 이용하여 계측하였다(참조: 도 1). 도 1은 마우스의 주름생성빈도를 나타내는 그래프이다. 도 1에서 보듯이, 화장품 A를 처리한 실험군은 대조군에 비하여,주름의 발생빈도가 약 70%정도 감소되었음을 알 수 있었다.The anti-wrinkle effect of the functional cosmetics prepared in Example 1 was examined as follows: First, a six-week-old nude mouse (albino SKH-1 hairless mice, Korean Experimental Animal Tester, Korea) was a UV box equipped with an ultraviolet lamp. It was put in, and irradiated with ultraviolet rays for 6 weeks once a day at a dose of 150mJ / cm 2 . Next, the cosmetic group prepared in Example 1 was treated with nude mice in an amount of 200 μl / cm 2, and the cosmetics having the same composition ratio as cosmetic A in the amount of 200 μl / cm 2 except for the absence of tannin component. Control groups treated with nude mice were compared and the results were measured using a skin replica kit (C + K, Germany) (see FIG. 1). 1 is a graph showing the frequency of wrinkles in the mouse. As shown in Figure 1, the experimental group treated with cosmetic A, compared to the control group, it can be seen that the incidence of wrinkles reduced by about 70%.
실시예 3: 기능성 화장품의 홍반형성 및 피부경화 억제효과 Example 3 effect of inhibiting erythema formation and skin hardening of functional cosmetics
자외선 조사전에 실시예 1에서 제조한 화장품 A를 200㎕/㎠의 양으로 누드마우스에 처리한 실험군과, 탄닌성분이 없는 것을 제외하고는 화장품 A와 동일한 조성비를 가지는 화장품을 200㎕/㎠의 양으로 누드마우스에 처리한 대조군에, 자외선을 150 mJ/cm2의 조사량으로 20분간 조사하는 조작을 1일 1회씩 10일간 반복하고, 매일 실험군과 대조군의 피부를 직접 스캐닝하여 홍반이 형성된 면적을 측정하여 비교하고(참조: 도 2), 피부의 경화정도를 측정하여 이를 비교하였다(참조: 도 3).The amount of 200 μl / cm 2 of the cosmetic group having the same compositional ratio as the cosmetic group A, except that there was no tannin component and the experimental group treated with the cosmetic A prepared in Example 1 in the amount of 200 μl / cm 2 before UV irradiation The control group was treated with nude mice for 20 minutes at a dose of 150 mJ / cm 2 for 10 minutes, and the area of erythema was formed by directly scanning the skin of the experimental group and the control group every day. (See FIG. 2), and the degree of hardening of the skin was measured and compared (see FIG. 3).
도 2는 자외선을 조사하기 전의 홍반면적을 기준으로 하여, 시간의 경과에 따라 생성된 홍반면적을 상대적으로 나타낸 그래프로서, 점선은 대조군을 나타내고 실선은 실험군을 나타낸다. 도 2에서 보듯이, 대조군의 경우, 3일 경과 후 홍반면적이 급격히 증가하여, 일정수준의 홍반이 계속적으로 유지되었으나, 실험군의 경우, 2일 경과 후 홍반면적이 증가하여 7일 후 정상수준을 유지하였으며, 최대홍반면적은 대조군의 최대홍반면적의 절반에 불과하였다.Figure 2 is a graph showing the erythema area generated over time, based on the erythema area before irradiation with ultraviolet rays, the dotted line represents the control group and the solid line represents the experimental group. As shown in FIG. 2, in the control group, the erythema area rapidly increased after 3 days, and a certain level of erythema was continuously maintained. However, in the experimental group, the erythema area increased after 2 days, thereby increasing the normal level after 7 days. The maximum erythema area was only half of that of the control group.
도 3은 자외선을 조사하기 전의 경화정도를 기준으로 하여, 시간의 경과에 따라 피부의 경화도를 상대적으로 나타낸 그래프로서, 점선은 대조군을 나타내고 실선은 실험군을 나타낸다. 도 3에서 보듯이, 대조군의 경우, 처음부터 피부의 경화도가 급격히 증가하여, 비교적 높은 수준으로 유지됨을 알 수 있었으나, 실험군의 경우, 피부의 경화도가 서서히 증가하여, 비교적 낮은 수준으로 유지됨을 알 수 있었다.3 is a graph showing the degree of curing of the skin over time, based on the degree of curing before irradiation with ultraviolet rays, the dotted line represents the control group and the solid line represents the experimental group. As shown in FIG. 3, in the case of the control group, the degree of sclerosis of the skin was increased rapidly and maintained at a relatively high level, but in the experimental group, the degree of sclerosis of the skin was gradually increased and maintained at a relatively low level. there was.
이상에서 상세히 설명하고 입증하였듯이, 본 발명은 항산화제로 알려져 있는 탄닌을 유효성분으로 함유하는 기능성 화장품을 제공한다. 본 발명의 탄닌을 유효성분으로 함유하는 기능성 화장품은 1 내지 10%(w/v)의 탄닌과 화장료를 포함한다. 본 발명의 탄닌을 유효성분으로 함유하는 기능성 화장품은 자외선에 의한 피부의 홍반침착 및 피부경화를 억제하므로, 피부손상의 예방 및 치료에 널리 활용될 수 있을 것이다.As described and demonstrated in detail above, the present invention provides a functional cosmetic containing tannin known as an antioxidant as an active ingredient. Functional cosmetics containing tannin of the present invention as an active ingredient includes 1 to 10% (w / v) of tannin and cosmetics. Functional cosmetics containing tannin of the present invention as an active ingredient inhibits erythema deposition and skin hardening of the skin by ultraviolet rays, and thus may be widely used for the prevention and treatment of skin damage.
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KR20040012361A (en) * | 2002-08-02 | 2004-02-11 | 영동특산영농조합법인 | Ink-Jet Ink Composition Include Extracted Tannin from Persimmon |
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JPS61109708A (en) * | 1984-10-31 | 1986-05-28 | Kobayashi Kooc:Kk | Cosmetic |
JPH0669933B2 (en) * | 1985-04-11 | 1994-09-07 | 鐘紡株式会社 | Skin cosmetics |
JPS63313707A (en) * | 1987-06-16 | 1988-12-21 | Seven Kagaku:Kk | Skin cosmetic |
JP2878353B2 (en) * | 1989-12-06 | 1999-04-05 | 株式会社資生堂 | External preparation for skin |
KR19980034827A (en) * | 1996-11-08 | 1998-08-05 | 성재갑 | Skin Whitening Composition |
JP3650245B2 (en) * | 1997-02-27 | 2005-05-18 | 御木本製薬株式会社 | Whitening cosmetics |
KR19990038400A (en) * | 1997-11-05 | 1999-06-05 | 성재갑 | Cosmetic composition for preventing skin aging |
KR20000047004A (en) * | 1998-12-31 | 2000-07-25 | 박인배 | Novel tyrosinase-inhibiting agent |
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KR20040012361A (en) * | 2002-08-02 | 2004-02-11 | 영동특산영농조합법인 | Ink-Jet Ink Composition Include Extracted Tannin from Persimmon |
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KR101440677B1 (en) * | 2012-07-11 | 2014-09-17 | 중앙대학교 산학협력단 | Novel Hydrolysable Tannis Isolated from the Leaves of Cleyera japonica Thunberg and Anti-Oxidative Use Thereof |
KR20190020530A (en) * | 2017-08-21 | 2019-03-04 | 충남대학교산학협력단 | Cosmetic Composition which comprises Diplectria barbata Extract as an active ingredient |
KR20200114654A (en) * | 2019-03-29 | 2020-10-07 | 대구대학교 산학협력단 | Skin whitening composition comprising gamma-ray irradiated kaki-tannin oligomer as effective component |
KR20200125833A (en) * | 2019-04-25 | 2020-11-05 | 농업회사법인주식회사 네이처팜 | Composition for anti-inflammation, anti-wrinkle and skin regeneration containing subcritical water-treated persimmon tannin as effective component |
KR102646357B1 (en) * | 2022-12-27 | 2024-03-12 | (주) 옵트바이오 | Tannin-containing plant extract-derived extracellular vesicles and use thereof for improving skin conditions |
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