KR20020067368A - The method of artificial cultivation for veiled lady mushroom - Google Patents

Abstract

PURPOSE: Provided is a method of artificial cultivation for veiled lady mushroom, thereby massively providing veiled lady mushroom which has abundant nutriments to promote nerve growth and to prevent obesity. CONSTITUTION: The method of artificial cultivation for veiled lady mushroom characterized by: using PBA medium extracted from potato and bamboo sawdust, as a growth medium of veiled lady fungus; using the black oak lumber until veiled lady mushroom is developed; cutting the black oak lumber into a short length, putting it into a thermostable vinyl bag and sterilizing it at 98-100 deg. C for 10 hours under atmospheric pressure; burying the black oak lumber then covering culture soil and rice straw thereon sequentially; removing the rice straw 10 days after veiled lady mushroom seeds are formed; and developing fruit bodies of the veiled lady mushroom 20-25 days after formation of veiled lady mushroom seeds.

Description

Artificial cultivation method of mantle mushroom {THE METHOD OF ARTIFICIAL CULTIVATION FOR VEILED LADY MUSHROOM}

The present invention relates to a method for artificially cultivating nutritious mango mushrooms,

More specifically, by artificially cultivating in order to provide a large amount of nutrients containing nutrients that promote neurodevelopment of children and the elderly and prevent obesity in women, the development of processed products by using the above-mentioned mushrooms In addition, it is possible to increase farmers' income through easy cultivation of mango mushrooms, thereby improving the economics of artificial cultivation of the whole mango mushrooms, and providing them at a low price by cultivating a large amount of mango mushrooms. The present invention relates to a method of artificial cultivation of mantle mushrooms to maximize reliability and satisfaction for users and producers of processed products.

Generally, under natural conditions, mantle mushrooms have a short survival time and are rarely easily collected.

The mantle mushroom belongs to the pile mushroom family, is an unusual kind of fruiting body develops under certain environmental conditions after forming eggs first in the process of fruiting body development.

That is, since the growth of mycelium is difficult to grow artificial mycelium, mushroom mycelial growth is fast, rich in nutrition and manufacturing technology of the medium is not easy to dry, and the mushroom fungus is slower than other mushrooms As the fruiting body is also generated in a unique condition, selection of a suitable material or control of physiological conditions should be appropriate, and environmental conditions at the time of generation and growth of manta mushrooms have a special difficulty in determining environmental conditions suitable for artificial cultivation.

Therefore, it is not easy to artificially cultivate the mantle mushroom has not yet a technology for artificial cultivation.

As a result, due to the difficulties described above, there is no technology for artificially cultivating mango mushrooms, and the development of processed products using the mango mushrooms is not active and at the same time, income is not increased for farmers who grow mushrooms, and prices are increased and consumed. There is a problem that the reliability and satisfaction are minimized for users and producers of processed products.

The present invention has been made to solve the difficulties and problems as described above has the following object.

The present invention by cultivating artificially to provide a large amount of nutrients containing nutrients to promote neurodevelopment of children and the elderly and to prevent obesity of women, to facilitate the development of processed products using the above-mentioned mushrooms, easy The cultivation of one mantle mushroom increases the farmer's income, thereby improving the economics of the artificial cultivation of the entire mantle mushroom, and providing it at a low price by cultivating a large amount of mantle mushroom. The purpose is to provide producers with artificial cultivation methods of manta mushrooms that maximize reliability and satisfaction.

Figure 1 is a photograph showing the eggs and fruiting bodies of manmade mushrooms artificially cultivated by the present invention.

Hereinafter, with reference to the accompanying drawings which are preferred embodiments of the present invention described above will be described in detail.

In the following description of the present invention, if it is determined that a detailed description of a related known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description thereof will be omitted.

In addition, the terms to be described below are terms set in consideration of functions in the present invention, which may vary depending on the intentions or customs of the producers producing the products, and the definitions should be made based on the contents throughout the specification.

First, the present invention, as shown in Figure 1, Figure 1 shows a picture showing the eggs and fruiting bodies of manganese mushrooms artificially cultivated by the present invention.

That is, the present invention uses a medium (PBA) extracted from potato and bamboo sawdust as a medium for growth of manta mushrooms.

Potassium extract medium (PDA) is generally used as a culture medium for culturing mushrooms, and MEA (Malt extract media), YMA (Yeast extract media) and MCM (Mushroom completemedia) are used. Growth was extremely slow, especially in the ASI 32003 strain with slow mycelial growth, it was difficult to remove the mycelium for the next experiment due to prolonged mycelial growth in PDA, YMA and MCM.

However, as a result of experiment in the medium (PBA) extracted from the potato and bamboo sawdust it can be seen that the mycelial growth rate is fast, the density of the mycelia is also high (see Table 1 below).

In addition, the acidity of the medium (PBA) extracted from the potato and bamboo sawdust can be seen in Table 2 below that pH 5.0 is appropriate.

Comparison of Mycelial Growth of Manta Mushrooms According to Different Types of Solid Medium Strain Mycelial growth by media type (mm / 14 days) PDA PPA BMA PBA MEA YMA MCM ASI 32002 38.3 ++++ 50.3 +++ 65.7 +++ 69.0 ++++ 50.7 ++++ 12.7 ++ 43.0 +++ ASI 32003 17.3 ++++ 20.3 +++ 30.7 ++ 26.7 ++++ 26.0 +++ 9.0 ++ 18.3 ++

PDA: Potato 200, Sugar 20, Agar 20 g / ℓ

PPA: Potato 200, Sugar 20, Peptone 1, KH ₂ PO ₄ 1, MgSO ₄ 0.5, Agar 20

BMA: Sawdust 20, Sugar 20, Malt extract 7, Peptone 1, KH ₂ PO ₄ 1, MgSO ₄ 0.5, Agar 20

PBA: Potato 200, Sawdust 20, Sugar 10, Malt extract 7, Peptone 1, KH ₂ PO ₄ 1, MgSO ₄ 0.5, Agar 20

MEA: Malt extract 20, Peptone 5, Agar 20

YMA: Yeast extract 3, Malt extract 3, Peptone 5, Sugar 10, Agar 20

MCM: Sugar 20, Yeast extract 2, KH ₂ PO ₄ 0.4, K ₂ HPO ₄ 1, MgSO ₄ 0.5, Agar 20

Mycelial density: + very weak, ++ weak, +++ high, ++++ very high

Mycelial Growth of Mantle Mushrooms with Different pH Strain Mycelial growth by medium pH (mm / 14 days) 4.0 5.0 6.0 7.0 8.0 9.0 ASI 32002 46 51 19 17 15 12 ASI 32003 22 26 12 13 10 8

Table 2 shows the experimental potato sawdust extract medium (PBA) at the culture temperature of 25 ℃.

In addition, the mantle mushroom is longer than the other mushrooms, the culture period is limited, fruiting conditions are limited and grown in the soil under conditions of high temperature and high humidity, it is cultivated in the medium material rather than sawdust, rice straw, waste cotton for a long time after incubation Logs that can be stored and survived until the fungus occurs due to burial in the soil are the best.

The types of logs include oak, pine and acacia, among which the logs of oak have higher culture period and culture completion rate, better growth density and mycelial growth than other logs, and the fruiting body of the manta mushroom is well formed. (See Table 3 below).

The mantle mushroom is cultivated by embedding it in the soil, so the length of the oak logs is important, and the shorter the length of the logs, the shorter the cultivation period, so it is advantageously cut into 10-20 cm for convenience (see Table 4 below). And, the cut oak solid wood is put into a heat-resistant vinyl (Hyden Film) bag and then made a pot to be sterilized by maintaining for 10 hours at a normal pressure of 98 ~ 100 ℃.

It can be seen that the 10 hours of normal pressure sterilization is shorter in culture period and higher in culture completion rate than 5 hours of normal pressure and 2 hours of high pressure (see Table 5 below).

Culture Characteristics of Manta Mushrooms According to Different Wood Species Tree species Incubation period (days) Culture completion rate (%) Mycelial density Mycelial Formation kind of oak 62 90 +++ +++ Pine tree 65 75 ++ + (Bacilli only on bark) acacia 80 40 + -(Delay of initial adhesion)

Table 3 above shows the results of 10 cm length of wood and 10 hours of autoclaving.

Cultivation Characteristics of Mango Mushrooms According to the Length of Wood Solid wood length Incubation period (days) Culture completion rate (%) Transfer period (days after sunk) Number of fruiting bodies (pcs / ㎡) 10 cm 50 90 45 135 20 cm 62 90 45 156

Table 4 shows the results of 10 hours of oak and atmospheric sterilization.

Culture Characteristics of Mantle Mushrooms According to Sterilization Methods Sterilization Method Incubation period (days) Culture completion rate (%) Atmospheric pressure 5 hours 65 50 10 hours of atmospheric pressure 62 90 High pressure 2 hours 70 75

Table 5 shows the results when the length of the oak logs 20 cm and sterilization temperature at 98 ~ 100 ℃, 121 ℃ at high pressure.

In addition, the oak wood, which has been cultured in the above, is buried in the ground and then covered with a clean masato over the wood cutting surface, and is thinly coated with rice straw to prevent drying.

At this time, the log must be buried upright, and the cover thickness should be covered by about 2cm from the cut surface of the log (see Table 6 below).

The soil for soil cover is advantageous for clean masato to maintain soil voids and soil moisture (see Table 7 below), and if the straw is coated with a thin layer of rice straw on the soil, the humidity of the soil surface and the mycelial and egg formation of the soil layer are formed. However, when the rice straw is covered until the fruiting body development period is continued, the mycelial growth and egg formation continue to die, so that the eggs of the mantle mushrooms start to form around 10 days to prevent this. Remove the rice straw covered in

After the egg is formed, the fruiting body is developed after 20 to 25 days if the environmental conditions are appropriate, and the vinyl house grower can harvest three cycles per year.

It is preferable that the temperature of the changing temperature is kept as small as possible by keeping the temperature of 20 ~ 25 ℃ in the growth period of the mycelia after burial the oak tree of the mycelia mushroom culture of the mantle mushroom in the vinyl house cultivator. To make the best use of the room temperature control in the range of 18 ~ 35 ℃, and to keep the indoor humidity of the egg and fruiting body generator to 90 ~ 98% (see Table 9 below).

Low humidity during egg growth causes cracks on the surface of the egg and stops the growth of eggs. Low humidity during the development of fruiting bodies results in severe malformations such as tearing of mushrooms during development.

Fruiting Body Characteristics of Manta Mushrooms According to Wood Burying Method and Covering Thickness division Investment method Cover thickness (cm) Bury Lying down 2 5 Egg formation quantity (piece / ㎡) 149 156 189 110 Fruit body generation amount (pcs / ㎡) 91 68 104 79 Fruiting body live weight (g / ㎡) 1074 918 1196 897

Table 6 above shows the result of when the oak logs are 20 cm long and the cover thickness test zone is buried.

Fruiting Outbreaks of Manta Mushrooms (ASI 32002) According to the Covering Type Cover type Egg (Volva) (piece /0.2㎡) Fruiting body sand 5 3 Masato 5 3

Rice straw cover effect on egg formation division Mycelial Injury Mycelia Egg formation (ea / ㎡) Normal Number of Growth (Pcs / 0.2㎡) Quantity (g / ㎡) Weight per site (g / piece) all Large pocket Teen and skirt Rice straw cloth ++++ +++ 223 100 1320 49.3 31.3 13.2 Rice straw removal +++ ++++ 115 108 1123 39.1 24.5 10.4

Table 8 above shows that the oak logs are 20 cm, and the depth of the cover is buried 2 cm.

Formation of fruiting bodies of Amanita mushroom (ASI 32002) according to temperature and humidity in a greenhouse division Room temperature (℃) Indoor humidity (%) Changing temperature (18-35) Calm temperature (22-25) 70-78 80-88 90-98 Egg formation Well done Poorly formed mycelial growth Delay in development Surface crack Well done Fruiting body development Well done Not deployed Not deployed Malformation Well done

Eventually, the mantle mushroom cultivated by the present invention can be mass-produced, and can use this to produce natural preservatives, cosmetics, and mushroom dishes, which can be a main income source for farmers, and the mantle mushrooms imported. All of them can be supplied domestically.

On the other hand, the present invention can be variously modified and take various forms in the cultivation method consisting of the above configuration.

It is to be understood, however, that the present invention is not limited to the specific forms referred to in the above description, but rather includes all modifications, equivalents and substitutions within the spirit and scope of the invention as defined by the appended claims. It should be understood to do.

As described above, the present invention, by artificially cultivating in order to provide a large amount of nutrients containing nutrients to promote neurodevelopment of children and the elderly and prevent obesity of women, the development of processed products using the above The effect of activating and increasing the farmer's income by the easy cultivation of mantle mushrooms improves the economics of the artificial cultivation of the mantle mushroom overall, and can be provided at a low price by cultivating a large amount of mantle mushrooms. It is obvious that this invention is a very useful invention that can simultaneously achieve various effects such as maximizing reliability and satisfaction to users and producers of processed products.

Claims (4)

Using a medium (PBA) extracted from potato and bamboo sawdust as a medium for growth of manta mushrooms, It is possible to store for a long time after the incubation and to be easily buried in the soil so that the germ culture period and culture completion rate are high, and the mycelial growth density and mycelial formation are good until the mantle mushroom is generated. Oak solid wood, Cut the length of the oak solid wood, and put the cut oak solid wood into a heat-resistant vinyl (Hiden film) bag and then make a pot for 10 hours at 98 ~ 100 ℃ atmospheric pressure sterilization, The oak logs, which have been cultured in the above, are buried in the ground, covered with clean masato over the cut surface of the oak logs, and then coated with a thin straw to prevent drying. If the rice straw is covered until the fruiting body development period, mycelial growth and egg formation continue, so that many eggs die, and to prevent this, the rice straw covered with the above is removed about 10 days after the egg starts to form. Artificial cultivation method of the mantle mushroom, characterized in that the fruiting body is developed after 20 to 25 days after the egg of the mantle mushroom is formed. The method of claim 1, The length of the oak solid wood artificial cultivation method of the mantle mushroom, characterized in that to run by cutting to 10 ~ 20cm to shorten the culture period. The method according to claim 1 or 2, The oak logs are erected and buried, the artificial cultivation method of the mantle mushroom, characterized in that to cover the cover thickness of about 2cm from the cut surface of the oak solid wood. The method of claim 1, The cultivation of the mango mushrooms to be harvested in a greenhouse house grower, After buried the oak logs of the mycelium mushroom cultured in the plastic house cultivator is completed to keep the geothermal temperature at 20 ~ 25 ℃ during mycelial growth, In the egg and fruiting body generator, the indoor temperature is controlled in the range of 18 ~ 35 ℃ by utilizing the natural conditions to the maximum. Indoor humidity of the egg and fruiting body generator is artificially cultivated method, characterized in that to carry out to maintain a high 90 ~ 98%.