KR20020050009A - Composition of cleansing agents for alleviating skin itching - Google Patents

Abstract

PURPOSE: A detergent composition for relieving and preventing skin itch containing an extract of Symphytum officinale L. Sophorae radix and Mentha piperita is provided which shows an excellent inhibiting effect on histamine release. CONSTITUTION: This detergent composition comprises 10% by weight of a Symphytum officinale L., 1% by weight of a Sophorae radix extract and 1% by weight of a Mentha piperita extract. The Symphytum officinale L. extract contains 0.01 to 10% by weight of allantoin, Sophorae radix extract contains 0.005 to 10% by weight of flavonoid, and Mentha piperita contains 0.05 to 5% by weight of menthol.

Description

Skin Itching & Suppressing Cleanser Composition {COMPOSITION OF CLEANSING AGENTS FOR ALLEVIATING SKIN ITCHING}

The present invention relates to skin itch relief and inhibitory cleanser compositions. More specifically, the present invention can relieve the itching that can be caused by various skin diseases by inhibiting the release of histamine, which is the main mechanism of skin itch, and the skin containing the extract, ginseng and peppermint extract A cleaning composition.

Itching is defined as the feeling of the skin that you want to scratch. This is a callus of several skin diseases and can be a serious condition in some patients. So far it has been found that there is no specific receptor for detecting itching, and it is thought to be due to unspecified autonomic nerve endings present in the dermo-epidermal junction between epithelial cells or the dermis and epidermis, such as local pain. Itching is produced by the very localized physical stimulation of the skin, and large-scale irritation acts as an inhibitor of the perception of itching. Scratching is a reaction phenomenon that relieves itching by activating the central inhibitor mechanism through myelinated 'A' fibroblasts. Itching, like pain, is transmitted through the slow flow of nonmyelination nociceptor 'C' fibroblasts through the dorsal horn of the spinal cord. Whether or not the pain-carrying halo and the itching-carrying are the same is still in dispute. Itching and pain are shown to pass along the same neurons. However, different excitation and suppression mechanisms in the central nervous system make them feel completely different sensations. The detection of itching is mainly by central control by excitatory and inhibitory neuronal circuits located in the substantial gelatinosa of the dorsal horn and by the opioid system. Severe itching after morphine epidural morphine is a well-known phenomenon, which can be antagonized by naloxoxne, in the presence of μ-opioid receptors in the central nervous system. Because. Itching in inflammatory skin diseases is due to the activity of inflammatory mediators including histamine protease, interleukin-2, substance P, opioid peptides, and the like. In addition, prostaglandin E does not act as an intermediary by itself but also enhances histamine activity. Histamine is the most well known substance that mediates itching and pain. It is mainly released from mast cells and by the cross linking of FCεR1α receptor with high affinity. Histimin is also inhibited by inflammatory mediators such as substance blood, stem cell factor (c-kit ligand), and vasoactive intestinal peptide, apart from the S.C. May be liberated from skin mast cells.

Therefore, there is a need for a method of suppressing and alleviating itching by effectively suppressing the release of histamine.

The present invention has been made to solve the above problems, the present inventors based on the mechanism of occurrence of itching published to date, the most known release of histamine as the main mechanism of the itch inhibition mechanism, effectively inhibiting the release of histamine An extract formulation was found and the present invention was completed.

Accordingly, an object of the present invention is to provide a composition capable of suppressing and alleviating itching by inhibiting the release of histamine.

In order to achieve the above object, the present invention provides a cleansing composition for mitigating and preventing itching, characterized in that it contains a Comfrey extract, Ginseng extract and Peppermint extract.

The extract and gosam extract is preferably obtained by cold settling at room temperature using an extraction solvent, the peppermint extract is preferably obtained by the steam distillation method.

The extractant is composed of purified water, methanol, ethanol, propanol, butanol, glycerol, propylene glycol, 1,3-butylene glycol, methyl acetate, ethyl acetate, benzene, hexane, diethyl ether, dichloromethane and mixtures thereof. It is preferably selected from the group.

In addition, the composition of the present invention preferably contains 0.1 to 10% by weight of Comfrey extract, 0.1 to 1% by weight of red ginseng extract and 0.01 to 1% by weight of peppermint extract, and most preferably, based on the total weight of the composition. , 10% by weight Comfrey Extract, 1% by weight Red Ginseng Extract and 1% by weight Peppermint Extract.

In addition, as an active ingredient to alleviate the itch, it is preferable that the Comfrey extract contains 0.01 to 10% by weight of allantoin, the red ginseng extract contains 0.005 to 10% by weight of flavonoids, and the peppermint extract contains 0.05 to 5% by weight of menthol.

Hereinafter, the present invention will be described in more detail.

General characteristics and pharmacological action of each ingredient

General properties and pharmacological actions of each component used in the composition of the present invention are as follows.

The name of the Comfrey is Symphytum officinale L. The name of the Comfrey is the dialect of con firma, meaning "bonding the bones", and the name Sumphytum is the Greek symbol "bonding". It comes from. A herbaceous plant, Borage is a perennial plant native to Europe and Asia, with several varieties. Like marshes and shades, planted under trees to grow well. The mature plants are 1.2 m in size, covered with thin hairs, and the outer leaves are 60 × 20 cm in size. As it has long been known under the name Sarassence Root (root of Saracens), the soldiers from the Crusaders' expedition were told with its utility. It has been popular since ancient times as a wound medicine, and it was well known as a osteopath in the Middle Ages.

The scientific name of Ginseng is Sophora flavescenes or Sophora angustifolia, and the herbal name is Sophorae radix. The ginseng has the shape of columnar or conical root, 5-20 cm in length, 2-3 cm in diameter, dark brown to yellow brown on the outer surface, clear vertical wrinkles, and yellowish white with fibrous skin. It also has a characteristic nausea, a bitter taste, and residuals. Alkaloids, which are the most important active ingredients, include matrin, oxymatrin, and sophoranol, and flavonoids include curaridin and isohydroicartin. ). In addition, the leaves contain luteonin-7-glucose, and the seeds contain cytisine and the like. The use of ginseng is used as a high-density agent, peripheral vasoconstrictor, antipyretic analgesic, and parasitic parasite. Toxicity is 0.4 g / kg, so avoid large doses. (Ref. Han Dae-seok; Pharmacology of the same name, 194-196, 1994 ).

Peppermint's scientific name is Mentha piperita, and there are more than 3,500 different species distributed in 180 genera around the Mediterranean. It was first cultivated near London, England in 1750, and was first used as an antiperspirant, a digestive aid that degassed the stomach. Most commonly used peppermint leaves contain 0.5-4% of volatile oils, of which 50-78% are single menthol and 5-20% are menthol components combined with other ingredients. When peppermint oil is applied to the skin, it is known to have analgesic and counterirritant effects that increase blood flow and reduce pain.

Preparation of Comfrey, Red Ginseng and Peppermint Extract

Referring to the extract of Comfrey, Ginseng, Peppermint used in the present invention.

Comfrey dried leaves, stems, and roots in a cow, cooled in about 10-fold extraction solvent for 5 days, and then filtered and concentrated to extract the extract. The ginseng was first purified and powdered from the root of red ginseng, chilled in about 10 times the extraction solvent for 5 days, and then filtered and concentrated to extract the red ginseng extract. Peppermint dried leaves and stems in the cow for several days and extracted by direct steam distillation. The extraction solvent used for the extraction of each raw material is purified water, methanol, ethanol, propanol, butanol, glycerol, propylene glycol, 1,3-butylene glycol, methyl acetate, ethyl acetate, benzene, hexane, diethyl ether, It is preferable to use one or two or more kinds of solvents in dichloromethane.

Histamine Free Inhibitory Evaluation Experiment Using Experimental Animals

For the evaluation of histamine free inhibitors, Sprague Dawley (male / 6-week-old) was used as an experimental animal and compound 48/80 was used as a histamine free trigger. First, select 4 locations to be injected into the skin of the back of the experimental animal, and place Compound 48/80 and various concentrations of Compri, Ginseng and Peppermint extracts in two of the four locations, and Compound 48/80 in the other two locations. Bay was injected. Comfrey, red ginseng and peppermint extracts were water extracts, and their concentrations were 1.0%, 0.1%, 0.01%, and 0.001%, respectively. 200 μl intravenous injection of 3% Evans blue, a reagent for staining histamine in the tail of the injected experimental animals, was allowed to stand for 1 hour, after which the epidermis was peeled off and only the blue stained area was cut with a knife. Cut out. Subsequently, it was immersed in 1 ml of 1 N KOH solution and reacted at 37 ° C. overnight to dissolve the tissue. To this was added 9 ml of a mixture of 0.6 M phosphoric acid and acetone (5:13) and centrifuged to remove tissue residues, and the supernatant was measured with a spectrophotometer at 620 nm.

% Inhibition = [(A-B) / A] × 100

A = amount of histamine stained with Evans blue (without extract)

B = amount of histamine stained on Evans blue (when extract is added)

Experimental results by the inhibition rate calculation method is shown in Table 1 below.

One 2 3 4 5 6 7 8 9 10 11 12 13 14 Comfrey Extract (%) 0.1 1.0 5.0 10.0 - - - - - - 10.0 10.0 10.0 - Red ginseng extract (%) - - - - 0.01 0.1 1.0 - - - 1.0 - 1.0 - Peppermint Extract (%) - - - - - - - 0.01 0.1 1.0 - 1.0 1.0 - % Inhibition 23.3 35.1 50.4 77.2 15.0 34.6 52.9 21.4 34.3 45.8 80.1 81.4 94.2 0

As can be seen from the results of Table 1, as a result of confirming the effectiveness of each of the extract and ginseng, peppermint extract and the mixture, when the mixture of the extract, ginseng, peppermint extract 10%, 1%, 1%, respectively, increase between extracts Itching effect was the best by the action.

Experiment to evaluate itching suppression using human

In order to confirm the anti-itch effect on the human skin of the compositions prepared in Table 1, after applying a pin chamber and a scanpore tape at a designated position of the back of 20 subjects After closing for 24 hours, it was removed. Then, the solution was pricked with a syringe niddle and left for one hour, and then the reaction degree was checked after 15 minutes by dropping a compound 48/80 1% solution, which is an itch-causing substance, drop by drop. The degree of reaction was checked by the degree of redness, and the longest diameter of the redness was measured and compared to the size thereof.

One 2 3 4 5 6 7 8 9 10 11 12 13 14 Redness size (cm) 2.7 2.5 2.0 1.0 2.9 2.4 1.9 2.7 2.2 1.8 0.8 0.7 0.2 3.2 Control percentage (%) 15.6 21.9 37.5 68.8 9.4 25.0 40.6 15.6 31.3 43.8 75.0 78.1 93.8 100

As can be seen from the results of Table 2, as a result of confirming the effectiveness of each of the extract and ginseng, peppermint extract and the mixture, the same as the results of Table 1, 10%, 1%, 1% of the extract, ginseng, peppermint respectively It was found that the best itching effect by the synergism between the extracts when mixed with%.

Validation and content evaluation of each extract

In order to confirm the active ingredient and anti-itch effect of each of the Comfrey, Ginseng and Peppermint extracts, the extracts transferred to each of the extracts and the non-polar solvent layer were developed into two-phase solvents systems, which were effective according to their polarities. It is effective to prevent itching by combining preparative solutions containing the same components by using column chromatography to separate and fractionate components and thin layer chromatography (TLC). The components were separated, and the active ingredients were finally identified by identification using analytical equipment such as mass and NMR. As a result of identifying and confirming the active ingredients of each extract, the extract solvent contained allantoin in an amount of 0.01 to 10% by weight, in red ginseng, flavonoids in an amount of 0.005 to 10% by weight, and peppermint in a menthol ingredient in a content of 0.05 to 5% by weight. It was confirmed that these were components exhibiting a major itching inhibitory effect. The anti-itching ability of each active ingredient was evaluated in humans and the like.

Allantoin Flavonoids menthol Blank Redness size (cm) 1.0 1.2 1.5 3.1 Control percentage (%) 67.7 61.3 51.6 100

As described above, the present inventors included the Comfrey, Ginseng, Peppermint extract in the composition of a body cleanser, soap, and evaluated the alleviation of itching and allergy. It was evaluated that the itching effect was excellent while the composition did not exhibit side effects such as skin redness.

Accordingly, the present invention provides a composition for preventing and alleviating itching by including the extract, ginseng, peppermint extract obtained by the above method in the cleaning composition, each composition based on the base of each product in general not containing excess fat Comprising the total composition of each composition, ginseng, peppermint extract 0.1 to 10% by weight, 0.01 to 1% by weight, characterized in that it contains 0.01 to 1% by weight.

The composition of the present invention may further include salicylic acid, glycyrrhetinic acid, alpha bisabolol, etc., which are substances capable of inhibiting inflammation, in order to increase the effect. In addition, the composition of the present invention may further include a flavor, a pigment, a fungicide, an antioxidant, a preservative, a moisturizing agent, and the like, and may further include a thickener, an inorganic salt, a synthetic polymer material, and the like to improve physical properties.

EMBODIMENT OF THE INVENTION Hereinafter, this invention is demonstrated based on an Example and a comparative example. However, these examples are only for illustrating the present invention, but the present invention is not limited thereto.

EXAMPLE

Examples 1-4 and Comparative Examples 1-4 (systemic cleaners)

Systemic cleaner base, Compri, ginseng, peppermint extract was added to each of the weight percent listed in Table 4 to prepare a systemic cleaner composition so that the composition is uniformly mixed through a process of stirring, heating, degassing, cooling in a mixer. Systemic cleansers containing comfrey, red ginseng and peppermint extracts can generally be prepared by convenient methods.

Raw material name Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 N-lauryl-L-glutamic acid triethanolamine (30% aqueous solution) 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 N-laurylmethyltaurine sodium (30% aqueous solution) 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 Sodium Lauryl Ether Sulfate 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 Polyglyceryl laurate 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Lauroyl diethanolamide 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Propylene glycol 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 Comfrey Extract 10.00 5.00 1.0 0.1 10.00 10.00 - 20.00 Ginseng extract 1.0 0.5 0.1 0.01 - 1.0 - 2.0 Peppermint Extract 1.0 0.5 0.1 0.01 1.0 - - 2.0 Purified water 21.00 27.00 31.80 32.88 22.00 22.00 33.00 9.00 Spices Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity dyes Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity Metal ion blockers, preservatives Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity

Histamine release inhibition experiment by topical application

In order to confirm the effect of preventing and treating the itch on the systemic cleanser composition prepared in Examples and Comparative Examples, each composition was applied twice a day for 3 days at a designated location of a laboratory animal (Sprague-Dawley) and 30 minutes After being left to rinse, compound 48/80 was injected into the site and 3% Evans Blue reagent was injected intravenously into the tail of the experimental animal. After 1 hour, lethality was extracted and the stained areas were extracted to evaluate histamine release inhibition.

The free inhibition rate of histamine is usually judged to have an itching effect only when the result is 20% or more. The histamine free inhibition test results are shown in Table 5.

Example 1 Example 2 Example 3 Example 4 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Histamine glass inhibition rate (%) 98.4 61.3 43.4 21.2 5.43 0.00 0.00 99.6

As can be seen in Table 5, the systemic detergent containing Compri, Ginseng, Peppermint extract was shown to have a superior histamine release inhibitory power than it does not contain it. As can be seen from the above results, the inhibitory power was slightly lower when the Comfrey, Ginseng and Peppermint extracts were added less than 0.1, 0.01, and 0.01% by weight, respectively, and the inhibition was more similar than that when 10% by weight was added. Thus, the content of Comfrey, Ginseng, Peppermint extract was excellent in the histamine free inhibitory force within the range of 0.01 to 10% by weight.

Examples 5-8 and Comparative Examples 5-8 (soaps)

Soap base (including moisture and various additives) and Comfrey, Ginseng and Peppermint extracts were mixed well in a mixer according to the weight percents shown in Table 6, and then extruded, cut and molded in a soap manufacturing machine to form a soap composition in the form of a solid soap. Prepared. The comfrey, ginseng, peppermint extract can be prepared by including not only general soap, but also synthetic soap and composite soap by a conventional soap manufacturing method.

Raw material name Example 5 Example 6 Example 7 Example 8 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 1.Soap base (including 13% moisture) 88.00 94.00 98.80 99.88 89.00 89.00 100.00 76.00 2. Comfrey Extract 10.00 5.00 1.0 0.1 10.0 10.0 - 20.0 3. Ginseng Extract 1.0 0.5 0.1 0.01 - 1.0 - 2.0 4. Peppermint Extract 1.0 0.5 0.1 0.01 1.0 - - 2.0 5. Spices Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity 6. Pigment Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity 7. Antioxidant (IDT) Quantity Quantity Quantity Quantity Quantity Quantity Quantity Quantity

Histamine release inhibition experiment by topical application

In order to confirm the effect of preventing and treating the itch on the soap compositions prepared in Examples and Comparative Examples, each composition was applied twice a day for 3 days at a designated position on the back of the experimental animal (Sprague-Dawley) for 30 minutes. After being left to wash, compound 48/80 was injected into the site and 3% Evans Blue reagent was injected intravenously into the tail of the experimental animal. After 1 hour, the lethal stained area was extracted to evaluate histamine release inhibition.

The inhibition rate of itching is determined to have a histamine release effect only when the result is 20% or more. The results of the histamine release inhibition experiment are shown in Table 7 below.

Example 5 Example 6 Example 7 Example 8 Comparative Example 5 Comparative Example 6 Comparative Example 7 Comparative Example 8 Histamine glass inhibition rate (%) 88.9 54.8 31.7 20.1 4.13 0.00 0.00 95.8 Workability Good Good Good Good Good Good Good Bad

As shown in Table 7, it was shown that the histamine free inhibitors were superior to those containing no soaps containing Comfrey, Ginseng and Peppermint extract. As shown in the above results, the inhibitory power was slightly lower when the Comfrey, Ginseng and Peppermint extracts were added less than 0.1, 0.01, and 0.01% by weight, respectively. Thus, the content of Comfrey, Ginseng, Peppermint extract was excellent in the histamine free inhibitory force within the range of 0.01 to 10% by weight.

As seen above, the cleaning composition of the present invention has an excellent inhibitory effect on the histamine glass, and thus is excellent in preventing and alleviating itching.

Claims (6)

It comprises a comfrey extract, ginseng extract and peppermint extract cleaning composition for itch relief and prevention. According to claim 1, wherein the Comfrey extract and ginseng extract is obtained by cold soaking at room temperature using an extraction solvent, the peppermint extract is a detergent composition for itching relief and prevention, characterized in that obtained by the steam distillation method. The method of claim 2, wherein the extraction solvent is purified water, methanol, ethanol, propanol, butanol, glycerol, propylene glycol, 1,3-butylene glycol, methyl acetate, ethyl acetate, benzene, hexane, diethyl ether, dichloromethane and Itching cleaning composition for alleviating and preventing itching, characterized in that it is selected from the group consisting of these. The itch of claim 1 or 2, comprising 0.1 to 10% by weight of Comprix extract, 0.1 to 1% by weight of red ginseng extract and 0.01 to 1% by weight of peppermint extract based on the total weight of the composition. Palliative and preventive cleaning composition. The composition of claim 4, wherein the composition comprises 10% by weight of Comfrey extract, 1% by weight of ginseng extract, and 1% by weight of peppermint extract, based on the total weight of the composition. According to claim 1 or claim 2, wherein as an active ingredient to reduce the itch, the Comfrey extract is 0.01 to 10% by weight of allantoin, the red ginseng extract is 0.005 to 10% by weight of flavonoids and the peppermint extract is 0.05 to 5% by weight It contains a%, cleaning composition for itch relief and prevention.