KR20020047762A - Cosmetics comprising sanguisorba root extracts for antioxidation - Google Patents

Abstract

PURPOSE: Provided are cosmetics comprising extracts of Sanguisorba officinalis L., for antioxidation. The extract of Sanguisorba officinalis L. show excellent free radical removing effects, therefore, the cosmetics comprising the extract has excellent prevention effects on wrinkle and skin aging. CONSTITUTION: Sanguisorba officinalis L. is extracted with C1-3 anhydrous or hydrous alcohol, or ethyl acetate at 4-30 deg.C for 3-20 days then the extract is concentrated, wherein diethylether soluble substances are removed therefrom before or after extraction process. The cosmetic contains 0.0001-5 wt.% of the extract in dried weight.

Description

Cosmetics comprising Sanguisorba root extracts for antioxidation

The present invention relates to an antioxidant (free radical scavenging) cosmetic containing a fat milk extract.

Free radical theory was proposed by D. Harman in the mid-1950s and has recently attracted attention in the academic and related industries. Radicals exist in the human body for a number of reasons. These radicals destroy cells, cut connective tissue in the dermal layer of the skin, or cause cross-linking, resulting in wrinkles, skin cancer, cell killing, rheumatoid arthritis, atopic dermatitis, It can cause various problems such as acne. Radicals are produced by the phagocytosis of leukocytes, the electron transport system during the production of ATP in mitochondria, the action of myeloper oxide (MPO), ultraviolet light, tobacco, normal metabolic processes, stress, pollutants, bacteria and the like.

Of course, there are antioxidants (superoxide dismutase (SOD), catalase, vitamin E, vitamin C, ubiquinol, etc.). Increasingly, however, antioxidant systems begin to break down due to age, pollution, ultraviolet light, and stress. Thus the antioxidant system is neutralized and the radicals increase gradually.

This increased radical destroys the dermal connective tissues of collagen, elastin, hyaluronic acid, and so on, causing subcutaneous subsidence of the skin (wrinkling), and also oxidizing the lipid part of the cell membrane to destroy cells. It causes symptoms such as dermatitis, acne, and skin cancer. In addition, this radical is involved in the spontaneous oxidation reaction (dopaquinone-> melanin) during melanin formation, causing blemishes, freckles, and wrinkles. Therefore, conventionally, ascorbic acid, α-tocopherol, SOD, and the like have free radical scavenging effects, and are used as a method of preventing wrinkles and other skin diseases by blending them in cosmetics or medicines. There has been a problem that the price is not only expensive but also difficult to obtain a practical effect because of poor chemical stability at the time of formulation.

The present inventors have solved the above problems, and as a part of the research to find a better raw material, as a result of searching several natural products, found that the extract of fat milk is excellent as a radical scavenger to complete the present invention.

Therefore, an object of the present invention is to provide an antioxidant (free radical scavenging) cosmetic containing a fat milk extract.

Antioxidant cosmetics according to the present invention comprises a fat-oil extract as a free radical scavenging component.

Fat milk refers to the root of the Sanguisorba officinalis L., a rose-ceae plant. Application of oil extract to skin has anti-inflammatory and vasoconstrictive effects.In herbal and folk remedies, it is used for astringents, hemostasis, addictive diarrhea, colitis, erythema, excessive inflammatory menstruation, postpartum hemorrhage, fibroid myoma, gum inflammation, stomatitis, etc. do. (Reference: Herbal Ingredients and Use)

In addition, the components of fat milk include tannin, gallic acid, ursolic acid, ellagic acid, sanguisorbin (C ₄₅ H ₆₂ O ₁₈ ), and fomolic acid. ), Quercetin glycosides, kaempfrol glycosides, and leucoanthocyanidins (see Chinese herbal metabolism, ingredients and use of herbs, new drug botany).

The fat milk extract may be prepared by extracting the dried fat milk at 4 to 30 ° C. for 3 to 20 days with anhydrous or hydrous alcohol having 1 to 3 carbon atoms, or ethyl acetate.

In addition, the fat milk extract may be prepared by extracting fat milk with water, anhydrous or hydrous alcohol having 1 to 3 carbon atoms, to ethyl acetate at 50 to 100 ° C. for 3 to 24 hours, and then concentrating.

On the other hand, the diethyl ether soluble component may be removed before or after performing the extraction process and then concentrated to prepare a more refined fat milk extract.

In the above extraction step, the fat oil is finely used, and the extraction solvent is added at a volume of 1 to 15 times the weight of the sample, and it is preferable that the extraction is carried out by mounting a cooling condenser to prevent evaporation of the active ingredient.

The fat milk extract prepared as described above may be added to conventional basic cosmetics, for example, softening water (skin), nutrient cosmetics (milk lotion), nutrition cream, massage cream, essence, pack, etc., to prepare an antioxidant cosmetic. The amount of the fat or oil extract used in the cosmetic is preferably 0.0001 to 5% by weight, preferably 0.001 to 3% by weight based on the weight of the cosmetic. At concentrations of 0.0001% by weight or less, it is difficult to obtain a substantial effect of radical elimination, and at concentrations of 5% by weight or more, the product stability is adversely affected.

Hereinafter, the present invention will be described in more detail with reference to the following Examples and Experimental Examples, but the present invention is not limited thereto.

Example 1

100 g of fat milk was finely chopped and placed in 1 L of 70% ethyl alcohol, which was extracted by dipping at room temperature for 10 days, filtered through 300 mesh filter paper, and allowed to stand for 1 week, and then the precipitate was filtered twice with filter sheet of EDVENTECH 7. Then, the resultant was concentrated completely at 50 ° C. using a vacuum concentrator to obtain a dry weight of 12.14 g.

Example 2

100 g of fat milk was finely chopped and placed in 1 L of 30% ethyl alcohol, which was extracted by dipping at room temperature for 10 days, filtered through 300 mesh filter paper, and allowed to stand for 1 week, and the precipitate was filtered twice with filter sheet of EDVENTECH 7. Then, the resultant was concentrated completely at 50 ° C. using a vacuum concentrator to obtain a dry weight of 15.08 g.

Example 3

100 g of fat milk was finely chopped, and placed in 1 L of 70% ethyl alcohol. The extract was boiled at 100 ° C. for 8 hours in an extractor equipped with a cooling condenser. The extract was filtered through 300 mesh filter paper and left for 1 week, and the precipitate was filtered twice with Filter No. 7 of Edbentech. Then, the resultant was concentrated completely at 50 ° C. using a vacuum concentrator to obtain a dry weight of 27.66 g.

Example 4

100 g of fat milk was finely chopped, and placed in 1 L of 30% ethyl alcohol. The extract was boiled at 100 ° C. for 8 hours in an extractor equipped with a cooling condenser. The extract was filtered through 300 mesh filter paper and left for 1 week, and the precipitate was filtered twice with Filter No. 7 of Edbentech. Then, the resultant was concentrated completely at 50 ° C. using a vacuum concentrator to obtain a dry weight of 25.08 g.

Example 5

100 g of fat milk was finely chopped, and placed in 1 L of 70% ethyl alcohol. The extract was boiled at 100 ° C. for 8 hours in an extractor equipped with a cooling condenser. The extract was filtered through 300 mesh filter paper and left for 1 week, and the precipitate was filtered twice with Filter No. 7 of Edbentech. The extract was mixed well by adding 1 L of diethyl ether, and then completely separated into two layers. Then, 1 L of ethyl acetate was added to the lower aqueous layer, mixed well, and then completely separated into two layers. This ethyl acetate layer was concentrated to 50 ° C. using a vacuum concentrator to give a dry weight of 5.95 g.

Experimental Example 1

The free radical scavenging effect was measured for Examples 1 to 5.

Experiments were carried out using the DPPH (1,1-diphenyl-2-picryl-hydrazyl) method (Blois, M.S. Nature 181, 1190, 1958), and DPPH was used by SIGMA. To 1 ml of 0.2 mM DPPH methanol solution, extracts of various concentrations of ethanol and 2 ml of methanol were added and stirred well, followed by reaction at room temperature for 10 minutes. Absorbance was measured at 517 nm, wherein purified water was used as a control instead of fat milk extract.

The free radical scavenging effect was obtained using Equation 1 below, and the results are compared with the free radical scavenging effect of ascorbic acid.

Free Radical Scavenging Effect of Fat Milk Extract Experimental substance Final experiment concentration (%) Free radical scavenging rate (%) SC ₅₀ Example 1 0.0014% 96.24% 0.00081% Example 2 0.0014% 92.40% 0.00078% Example 3 0.0014% 95.97% 0.00077% Example 4 0.0014% 94.02% 0.00082% Example 5 0.0009% 95.32% 0.00043% Ascorbic acid 0.005% 91.28% 0.0009%

SC ₅₀ : concentration of the sample that eliminates 50% of free radicals (50% scavenging

Concentration

As can be seen in Table 1, the free radical scavenging effect of the fat milk extract was superior to the conventional ascorbic acid.

Prescription Example 1

Prescription examples of the flexible cosmetics (skin) of the cosmetics containing a fat milk extract is as follows. The fat milk extract here refers to that of Example 1.

ingredient Content (% by weight) Butylene Glycol Glycerin Carboxyvinyl Polymer Disodium Ethylenediaminetetraacetic Acid Ethanol Triethanolamine Solbitan Monostearate Hydrogenated Castor Oil Phenyltrimethicone Preservatives Flavors 4.08.00.060.017.00.20.80.43.50.40.10.01 balance 5.0 system 100

Prescription Example 2

Prescription example of a lotion in the cosmetic composition containing a fat milk extract is as follows. The fat milk extract here refers to that of Example 1.

ingredient Content (% by weight) Stearic acid Cetyl alcohol Glyceryl monostearate Polyoxyethylene sorbitan monostearate sorbitan sesquioleate liquid paraffin squalane carlylic / capric triglyceride carboxyvinyl polymer butylene glycol triethanolamine preservative fragrance colorant resin oil extract 1.01.01.01.01.04.03.04.00.15.00.20.40.10.01 Balance 5.0 system 100

Prescription Example 3

Prescription example of nutrition cream in the cosmetic composition containing the fat milk extract is as follows. The fat milk extract here refers to that of Example 1.

ingredient Content (% by weight) Stearic acid cetyl alcohol glyceryl monostearate polyoxyethylene sorbitan monostearate sorbitan sesquilate glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate wax fluid paraffin squalane carlylic / capric triglyceride carboxyvinyl Polymer Butylene Glycol Glycerine Triethanolamine Preservatives Flavoring Pigment Resin Resin Oil Extract 2.02.02.00.50.51.01.04.04.04.00.35.03.00.50.40.10.01 Balance 5.0 system 100

Since the oil extract according to the present invention has an excellent free radical scavenging effect, it can provide an excellent antioxidant cosmetic for preventing wrinkles and skin aging.

Claims (6)

Antioxidant cosmetic containing fat extract. The anti-oxidant cosmetic according to claim 1, wherein the fat or oil extract is prepared by extracting the fat or oil with anhydrous or hydrous alcohol having 1 to 3 carbon atoms to ethyl acetate for 3 to 20 days, and then concentrating. The anti-oxidant cosmetic according to claim 1, wherein the fat milk extract is prepared by extracting fat milk with water, anhydrous or hydrous alcohol having 1 to 3 carbon atoms to ethyl acetate for 3 to 24 hours at 50 to 100 ° C, and then concentrating. . The antioxidant cosmetic composition according to claim 2 or 3, wherein the fat or oil extract is prepared by removing the diethyl ether soluble component before or after performing the extraction process and then concentrating. The antioxidant cosmetic composition according to claim 1, wherein the fat or oil extract is contained 0.0001 to 5% by weight in a dry weight. The antioxidant cosmetic composition according to claim 1, wherein the fat or oil extract is added to the basic cosmetic selected from a flexible lotion, a nourishing lotion, a nourishing cream, a massage cream, an essence or a pack.