KR20010100728A - Manufacturing method of foods in silk with enzyme - Google Patents

Manufacturing method of foods in silk with enzyme Download PDF

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Publication number
KR20010100728A
KR20010100728A KR1020000024253A KR20000024253A KR20010100728A KR 20010100728 A KR20010100728 A KR 20010100728A KR 1020000024253 A KR1020000024253 A KR 1020000024253A KR 20000024253 A KR20000024253 A KR 20000024253A KR 20010100728 A KR20010100728 A KR 20010100728A
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enzyme
silk
fibroin
amino acid
manufacturing
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KR1020000024253A
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Korean (ko)
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김정수
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김정수
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C07K14/43586—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Insects & Arthropods (AREA)
  • Birds (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

누에고치(실크)에서 식용불가능한 부분인 세리신을 제거하고 피브로인을 얻는 단계와 ; 이때 얻어지는 피브로인에 효소가 잘 침투할 수 있도록 조건을 만들어 주는 단계와 ; 피브로인에 효소를 작용시키는 단계와 ; 이를 일정시간과 일정온도에서 반응시켜 식용으로 할 수 있는 방법(아미노산 제조)을 제공한다.Removing the edible sericin from the cocoon (silk) and obtaining fibroin; Making conditions so that the enzyme can penetrate the fibroin obtained at this time; Acting an enzyme on fibroin; It provides a method (made by amino acid) that can be made edible by reacting this at a certain time and a constant temperature.

Description

효소에 의한 실크의 식용화 방법{MANUFACTURING METHOD OF FOODS IN SILK WITH ENZYME}Method of Edible Silk by Enzyme {MANUFACTURING METHOD OF FOODS IN SILK WITH ENZYME}

본 발명은 누에고치(실크)에서 아미노산 제조방법에 관한 것인데, 일반적으로, 누에고치(실크)에서 식용할 수 없는 세리신을 제거하기 위해 가성소다를 사용하고 있다.BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for producing amino acids in cocoon (silk). Generally, caustic soda is used to remove edible sericin from silkworm cocoon (silk).

또한, 이때 얻어지는 피브로인으로부터 아미노산을 얻기 위해 염산으로 가수분해하여 가성소다로 중화하는 방법을 쓰고 있다.In addition, in order to obtain an amino acid from the fibroin obtained at this time, a method of hydrolyzing with hydrochloric acid and neutralizing with caustic soda is used.

이러한 방법은 제조공정이 쉽고 비용이 적게 드는 반면, 생산량이 적게 얻어지는 단점이 있고, 또 화학약품을 사용한 방법이어서 거부반응을 이르킬 수 있는 문제점이 있다.While this method is easy and inexpensive in manufacturing process, there is a disadvantage in that a small amount of production is obtained, and there is a problem in that it is a method using chemicals that can lead to rejection.

본 발명은 상술한 바와 같은 화학약품에 의한 거부반응을 방지하고, 질좋은 아미노산을 제조하기 위한 것이다.The present invention is to prevent rejection by the chemicals described above, and to produce a quality amino acid.

특히, 본 발명은 누에고치(실크)가 갖는 당뇨, 고혈압, 뇌졸중 , 간기능, 노인성치매, 성기능장애, 성장장애 등을 개선할 수 있는 우수한 기능성을 갖고 있으며, 산업적으로도 인공혈관, 콘텍트렌스, 수술봉합사 등의 의료기구와 화장품제료 등으로 용도가 많은 아미노산과 그 제조방법을 제공하기 위한 것이다.In particular, the present invention has excellent functionality to improve diabetes, hypertension, stroke, liver function, senile dementia, sexual dysfunction, growth disorders, etc. that cocoon (silk) has, industrially artificial blood vessels, contact, To provide a large number of amino acids and a method of manufacturing the same as a medical device such as surgical suture and cosmetics.

이러한 목적을 이루기 위하여, 본 발명은 (가) 누에고치로에 탄산나트륨을 반응시켜 식용불가능한 세리신을 제거하고 피브로인을 얻는 단계와; (나) 이때 얻어진 피브로인을 염화칼슘을 써서 단단한 구조로부터 효소가 잘 침투할 수 있도록 느슨하게 해 주는 단계와; (다) 상기 피브로인에 효소를 첨가한는 단계와; (라) 상기 (다)단계를 거친 재료를 일정시간, 일정온도에 의해 식용 할 수 있는 아미노산을 제조하는 제조방법과 이러한 방법을 사용한 아미노산을 제공한다.In order to achieve this object, the present invention comprises the steps of: (a) reacting the cocoon with sodium carbonate to remove inedible sericin and to obtain fibroin; (B) loosening the fibroin obtained at this time using calcium chloride to allow the enzyme to penetrate well from the rigid structure; (C) adding an enzyme to the fibroin; (D) It provides a manufacturing method for producing an amino acid that can be edible at a predetermined temperature, a material passed through the step (c) and a amino acid using the method.

이하, 본 발명의 바람직한 실시예를 구체적으로 설명한다.Hereinafter, preferred embodiments of the present invention will be described in detail.

누에고치(실크)로부터 잡물을 제거하고 일정량을 1%정도의 탄산나트륨용액에 넣어 95℃를 유지하면서 2시간을 처리한 다음 충분한 물로 수세한다. 다음 이것을 탈수하고 자연건조시켜 피브로인을 얻는다. 이때 얻어진 피브로인은 그 구조가 매우 단단하여 느슨한 구조를 만들기 위해 30%농도의 염화칼슘용액을 만들어 여기에 피브로인을 넣어 90℃로 10시간을 반응시킨다. 그리고는 셀룰로스 투석 방법 등으로 염을 제거한다. 즉, 증류수를 수시로 갈아주면서 48시간을 교반시킨다.Remove the miscellaneous material from silkworm cocoon (silk), add a certain amount to 1% sodium carbonate solution, treat it for 2 hours while maintaining 95 ℃, and wash with plenty of water. This is then dehydrated and air dried to obtain fibroin. The fibroin obtained at this time is very hard to make a loose structure, 30% calcium chloride solution is made to put the fibroin and reacted for 10 hours at 90 ℃. The salt is then removed by cellulose dialysis or the like. That is, stirring 48 hours while changing the distilled water from time to time.

이렇게해서 중성염 등 불순물이 제거된 단백질에 분해효소인 플레보자임(Flavourzyme)을 시료의 0.8%정도 넣어 50℃를 유지하면서 10시간 분해시킨다.Thus, flavozyme, a degrading enzyme, was added to a protein from which impurities such as neutral salts were removed, and then decomposed for 10 hours while maintaining the temperature at 50 ° C.

그리고는 이액을 농축한 다음 동결건조시키면 펩티드 및 아미노산인 가루가얻어진다. 이렇게 제조된 아미노산은 다음과 같은 방법으로 그 종류와 함량을 측정하였다. 즉, 시료를 원심분리에 의한 침전물을 제거한 다음 시료의 일정량을 취하여 TFA용액 20㎖를 가하여 단백질을 제거한 후 이 용액20㎕를 취하여 HPLC로 분석했다.The concentrate is then concentrated and lyophilized to yield a powder that is a peptide and an amino acid. The amino acid thus prepared was measured in the following manner and its content. In other words, the precipitate was removed by centrifugation, and then a predetermined amount of the sample was taken, 20 ml of TFA solution was added to remove the protein, and 20 µl of the solution was taken and analyzed by HPLC.

상술한 바와 같은 누에고치(실크)로 부터의 아미노산 분석 결과는 다음과 같다.The results of amino acid analysis from the cocoon (silk) as described above are as follows.

(mg%) (mg%)(mg%) (mg%)

아스파틴산 2.2 글르타민산 5.4Aspartic Acid 2.2 Glutamic Acid 5.4

아스파라긴 0.9 세린 26.4Asparagine 0.9 Serine 26.4

트레오닌 2.0 프롤린 2.2Threonine 2.0 Proline 2.2

티로신 4.7 발린 3.9Tyrosine 4.7 Valine 3.9

메티오닌 58.0 이소루신 1.8Methionine 58.0 Isoleucine 1.8

루신 3.1 페닐알라닌 3.0Leucine 3.1 Phenylalanine 3.0

라이신 2.8Lysine 2.8

Total 116.4Total 116.4

상기한 바와 같이 본 발명에서는 누에고치(실크)로부터 13종류의 아미노산을 얻을 수 있었다.As described above, in the present invention, thirteen kinds of amino acids were obtained from silkworm cocoons (silk).

상술한 바와 같이 본 발명에 따라, 누에고치(실크)를 아미노산으로 분해함에 있어서 기존의 염산에 의한 분해 방법보다는 본 방법인 효소에 의한 방법은 위생적인 방법이어서 선호도면 뿐아니라 생산량도 많이 얻을 수 있는 잇점이 있다. 특히 본 발명은 당뇨, 고혈압, 뇌졸중 , 간기능, 노인성치매, 성기능장애, 성장장애 등의 예방, 치료에 효과적일 뿐아니라, 산업적으로도 인공혈관, 콘텍트렌스, 수술봉합사 등의 의료기구와 화장품제료 등에 이용될 수 있는 재료가 되는 그러한 아미노산 제조 방법이다.According to the present invention as described above, in the decomposition of silkworm cocoon (silk) into amino acids, the method by the enzyme of the present method rather than the conventional decomposition method by hydrochloric acid is a hygienic method can be obtained a lot of production as well as preferences There is an advantage. In particular, the present invention is effective in the prevention and treatment of diabetes, hypertension, stroke, liver function, senile dementia, sexual dysfunction, growth disorders, etc., as well as medical devices and cosmetic preparations such as artificial blood vessels, contacts, surgical sutures, etc. It is such an amino acid manufacturing method which becomes a material which can be used etc.

Claims (1)

(가) 누에고치에서 세리신을 제거하여 피브로인을 얻는 단계와 ;(A) removing sericin from the cocoon to obtain fibroin; (나) 피브로인의 구조를 느슨하게하여 효소의 침투를 용이하게하는 단계와 ;(B) loosening the structure of fibroin to facilitate penetration of the enzyme; (다) 분해 효소를 첨가하는 단계와 ;(C) adding a degrading enzyme; (라) 상기 (다)단계를 거친 재료를 일정시간과 일정온도를 반응시켜 아미노산을 제조하는 아미노산 제조방법.(D) Amino acid production method for producing an amino acid by reacting the material passed through the step (c) a predetermined time and a predetermined temperature.
KR1020000024253A 2000-05-06 2000-05-06 Manufacturing method of foods in silk with enzyme KR20010100728A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010079285A (en) * 2001-07-03 2001-08-22 주육현 Method of production by enzymatic digestion of functional silk amino acid-peptide materials using cocoon
KR100476716B1 (en) * 2001-12-08 2005-03-16 이승수 A health foods using vegetable worms of making the blood circulate more freely and It's manufacturing method
WO2006014033A1 (en) 2004-07-31 2006-02-09 Brainguard Co., Ltd. Silk peptide improving neuroprotective and neurofunctional effects and a method of its preparation
WO2013032411A1 (en) * 2011-08-26 2013-03-07 Agricultural Research Development Agency (Public Organization) Silk-based bioactive oligopeptide compositions and manufacturing process therefor
KR102107816B1 (en) * 2019-12-09 2020-05-07 주식회사 네이처센스 농업회사법인 Silk-derived peptide composition for improving cognitive function and memory

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010079285A (en) * 2001-07-03 2001-08-22 주육현 Method of production by enzymatic digestion of functional silk amino acid-peptide materials using cocoon
KR100476716B1 (en) * 2001-12-08 2005-03-16 이승수 A health foods using vegetable worms of making the blood circulate more freely and It's manufacturing method
WO2006014033A1 (en) 2004-07-31 2006-02-09 Brainguard Co., Ltd. Silk peptide improving neuroprotective and neurofunctional effects and a method of its preparation
EP1773864A4 (en) * 2004-07-31 2009-02-18 Biogrand Co Ltd Silk peptide improving neuroprotective and neurofunctional effects and a method of its preparation
AU2004322006B2 (en) * 2004-07-31 2012-05-24 Brainguard Co., Ltd. Silk peptide improving neuroprotective and neurofunctional effects and a method of its preparation
US10010574B2 (en) 2004-07-31 2018-07-03 Brainguard Co., Ltd. Silk peptide for improving neuroprotective and neurofunctional effects and a method of its preparation
WO2013032411A1 (en) * 2011-08-26 2013-03-07 Agricultural Research Development Agency (Public Organization) Silk-based bioactive oligopeptide compositions and manufacturing process therefor
KR102107816B1 (en) * 2019-12-09 2020-05-07 주식회사 네이처센스 농업회사법인 Silk-derived peptide composition for improving cognitive function and memory

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