KR20010078917A - The new Bacillus sp. having an excellent digestive effect of organic material and the treatment method of food waste - Google Patents

Abstract

PURPOSE: A novel Bacillus sp. strain having improved decomposability of organic materials and a method for treating food wastes using the same are provided, therefore, the food wastes can be effectively and easily treated and reused. CONSTITUTION: The novel Bacillus sp. strain GENO-200 (KCCM-10,263) decomposing organic materials is isolated from soil by the steps of: collecting soil, inoculating in an NB broth and incubating them at 37 deg. C for 5 days; determining and comparing the productabilities of amylase and protease for the fermented culture; and selecting a strain and identifying the strain as Bacillus sp. GENO-200. The method for treating food wastes using the novel strain comprises the steps of: mixing Bacillus sp. GENO-200 or its fermented culture with wood chip; fermenting the mixture at 30 to 40 deg. C for 1 to 5 hours; removing water and adding food wastes into the water removed mixture; fermenting the mixture for 1 day and continuously adding food wastes and Bacillus sp. GENO-200 or its fermented culture for 1 month to decrease the weight of food wastes and reuse the remnant as an organic fertilizer.

Description

New Bacillus sp strains with excellent organic decomposition ability and food waste treatment method using the same {The new Bacillus sp. having an excellent digestive effect of organic material and the treatment method of food waste}

The present invention relates to a novel Bacillus sp strain having excellent organic matter degrading ability and flame resistance and heat resistance, and a food waste treatment method using the same.

In general, a large amount of food waste is generated in each household or restaurant, which amounts to 25 to 35% of the total amount of household waste, and such food waste contains an excessive amount of water, which causes considerable cost and difficulty in processing. In addition, there was a problem that bad smell occurs when the organic matter of food waste is decomposed and various pathogens grow natively, which greatly affects hygiene.

As a conventional food waste treatment method for solving the problems as described above, a method of incineration by using a landfill method and an incinerator is widely applied.

However, when burying food waste, a large amount of leachate is inevitably generated, which not only contaminates the surrounding environment but also groundwater, and causes economic problems due to securing and establishing a landfill.

In addition, the incineration of food waste requires a lot of energy due to the incineration of the nature of the wet waste, which incurs a lot of expenses and the harmful effects on the human body due to the generation of toxic substances such as dioxin when incorporating aromatic chemicals. Of course, there was a problem causing serious environmental pollution.

In addition, recently, a method of forcibly drying the moisture contained in food waste by using a dryer to reduce the amount of food waste, a method of recycling into fertilizer or feed, and a method of extinguishing food waste by using microorganisms, etc. It is proposed.

However, the drying method is causing secondary pollution problems due to the discharge of waste gas and waste water, which is harmful to the human body, and requires a large amount of water to be dried, which requires excessive costs, and recycles it as a fertilizer or feed and uses microorganisms. In the method of extinguishing food waste, it is avoided due to severe odor generated when organic matter is decomposed and its efficacy is not satisfactory, so it is rarely used in actual use. Due to the development of a disease known to be harmful to the human body, it is rarely used as a feed recently. In particular, the composting method has been positively evaluated in terms of recycling, but the degradation of the soil due to the accumulation of salinity in the residue has been raised as a problem.

On the other hand, in the method of extinguishing food waste using microorganisms, since food waste has a high salt concentration, it is not easy to grow microorganisms. This is not only a problem that is excessively consumed, but also the treatment efficiency is not good, and in the case of microorganisms that do not grow at a high temperature due to heat generation as food is decomposed by microorganisms, it does not express a satisfactory effect on continuous food waste disposal. there was.

It is therefore an object of the present invention it has excellent salt tolerance and heat resistance while the organic resolution superior to produce a-amylase and protease at the same time as well as a continuous food waste processing be processing efficiency novel Bacillus suitable for excellent food waste treatment SP (Bacills sp ) Strains.

Another object of the present invention to provide a method of treating food waste using a novel Bacillus sp (Bacills sp) strain of the object.

In order to achieve the above-mentioned objects as well as other objects that are easily expressed, the present invention isolates and cultivates the strains from the seashore soils to obtain a culture solution, and the organic matter resolution is measured based on the pharmacopeia standard. As well as having an organic decomposition ability as well as excellent flame resistance and heat resistance it was found that the effect is excellent in the treatment of food waste.

1 is a perspective view showing an example food waste treatment apparatus used in the present invention.

2 is a partial cutaway perspective view of the food waste treating apparatus of FIG. 1.

Figure 3 is a cross-sectional view showing the configuration of the air injection device in the food waste treatment apparatus of FIG.

Figure 4 is a cross-sectional view showing the configuration of the deodorizing means in the food waste treatment apparatus of FIG.

<Description of the symbols for the main parts of the drawings>

10: shredder 20: feeder

21: screw 30: decomposition reaction tank

31: stirring device 33: opening

35: sliding plate 40: discharge means

41: first screw 42: joint

43: second screw 44: drive motor

45 housing 46 discharge pipe

47: cap 50: filter box

60: air injection device 61: air inlet hole

62: blocking plate 63: blowing fan

65: thermometer sensor 67: heater

70: deodorization means 71: on-off valve

73: intake pipe 75: first branch pipe

77: second branch 79: exhaust pipe

80: first deodorizer 80a: second deodorizer

81, 81a: activated carbon fiber 83, 83a: heating coil

90: ceramic heating wire

By simultaneously producing amylase and protease according to the present invention, it has excellent flame retardancy and heat resistance, and has excellent bactericidal and heat resistance, and is capable of continuously processing food waste as well as new Bacillus sp GENO- suitable for treating food waste with excellent treatment efficiency. The 200 strain is a simple bacilli and has a cell size of 1.1 × 3.8 μm, has a motility, shows a positive response to gram staining, forms an elliptical spore at the center, and has a growth temperature range of 15 to 65 ° C. and a salt concentration of 25%. It is capable of growing up to, catalase positive, starch, casein cellulose degradability, amylase titer is about 6,000 units / ml, protease titer is characterized by about 4,000 units / ml.

In addition, the food waste treatment method according to the present invention is mixed with a wood chip (wood chip), such as sawdust or the like microorganism strain or aged for 1 to 5 hours at 30-40 ℃, then dehydrated and shredded food waste After the addition, it is characterized by culturing for one day, and then the food waste and the microbial strain or culture from the next day and continuously treated to reduce the food waste for one month and the remaining amount is reused as organic fertilizer.

In addition, in addition to the Bacillus sp . GENO-200 strain in the above-mentioned food waste treatment method, if the microorganisms having excellent lipase generating ability and microorganisms having high fibrinolytic ability are treated together, the weight loss of food waste is further enhanced.

Bacillus sp . GENO-200 strain of the present invention is isolated from soil close to the beach. The culture medium obtained by culturing the strain in a liquid medium produces high titers of amylase and protease among digestive enzymes to promote the digestion of food waste, as well as high salt concentration and excellent growth at high temperature, in the treatment of food waste. It is possible to solve the problem of killing microorganisms due to the increase in temperature as the pretreatment or digestion proceeds to reduce the salt concentration in question.

Therefore, the use of the strain or the culture of the present invention can be easily processed to reduce the food waste 95% or more.

In the food waste treatment method of the present invention, wood chips are not particularly limited, and it is effective to use readily available sawdust, wood chips and Bacillus sp . GENO-200 strain or culture. The ratio of wood chips: cultures = 20 to 50: 1 based on the silver culture was effective for the maturation of microorganisms as well as the digestion of food waste in the future.

Maturation Bacillus sp . GENO-200 strain is to be adapted to the food treatment apparatus, it is preferable to perform for 1 to 5 hours at 30-40 ℃.

Food waste should be dehydrated and crushed. In some cases, if the salt concentration is too high, it may be used by washing with water.

Food waste is put into 50 ~ 100 ㎏ every day, and if it exceeds 100 ㎏ there is a problem that the treatment efficiency is lowered or the processing time is excessively consumed, if less than 50 ㎏ it is a microorganism due to depletion of nutrients There is a disadvantage that the growth of the food waste is not satisfactory in the future, the treatment efficiency of food waste is reduced.

In addition, microorganisms excellent in the ability to generate lipase and microorganisms in which the cellulose decomposing ability is additionally used in the food waste treatment method are not particularly limited, but one or more kinds of lipase excellent in producing ability and deodorizing ability may be used. In the present invention, Candida Cylindracea ATCC 1483 was used as a microorganism having excellent lipase production, and Thermobifida Fusca ATCC 27730 was used as a microorganism having excellent fibrinolytic ability .

In the present invention, the titer of protease and amylase was measured by the following method.

※ protease

0.6% casein solution (0.6 g of casein is precisely weighed, dissolved in 20 ml of 0.1 N NaOH solution, dissolved and cooled, and then adjusted to pH 7.2 with 0.1 N phosphoric acid and 100 ml of water). 1 ml of the culture diluted at 2,000 times was added and allowed to react for 10 minutes. Then, 2 ml of 0.4M trichloroacetic acid solution (65.4 g of trichloroacetic acid dissolved in water to 1 l) was added thereto and left for 25 minutes, followed by 1 ml of filtrate, 5 ml of 0.4M sodium carbonate solution (42.4 g of sodium carbonate (primary) in 1 liter of water) is mixed with 1 ml of polyline solution and left for 20 minutes, and the absorbance E is measured at 660 nm.

Separately, take 1 ml of the culture diluted 2,000 times and put it into the test tube, mix 2 ml of 0.4M trichloroacetic acid solution, mix it, add 1 ml of 0.6% casein solution, filter it after 10 minutes, and filter with 1 ml of filtrate. Measure the absorbance E _o .

Separately, make tyrosine standard at 50 µg / ml, take exactly 1 ml of this solution and color it with a culture solution to measure absorbance E _s .

Protease titer = (E-E _o ) / E _s × 50 × 4/10 × dilution factor

※ amylase titer

2% soluble starch solution (2 g of Merck starch is mixed with a small amount of water, slowly added to 50 ml of boiling water and continuously boiled for 5 minutes, and cooled to 10 ml with 10 ml of Mcilvain buffer at pH 5.6 and water) Preheat 10 ml at 40 ° C for 10 minutes, add 1 ml of 25,000-fold diluted culture, and react for 30 minutes. Add 4 ml of ferring solution (34.660 g of copper sulfate dissolved in water to 500 ml) and place on a direct fire. Boil for 5 minutes, cool in running water, and shake with 2 ml of 25% alkaline tartarate (173 g of potassium citrate and 50 g of sodium hydroxide dissolved in water to make 500 ml) and 2 ml of 25% sulfuric acid. Titrate with sodium sulfate solution (12.409 g of sodium thiosulfate dissolved in water to 1 l) and perform a blank test using water instead of culture.

Amylase titer = 1.62 × (test value-measured value) × 0.1 × dilution factor × f (regularity coefficient of 0.05 N sodium thiosulfate)

The food waste treatment apparatus used in the present invention used an apparatus as shown in FIGS. 1 to 4.

That is, the control unit 6 is installed on one side of the main body 2 having a main body 2 formed with an inlet 5 that is selectively opened and closed by the lid 4 to inject food, and the control unit 6 Is operated by an operation unit (not shown) provided on the upper one side of the main body (2).

The main body 2 has a built-in shredder 10 for cutting, crushing and crushing the input food waste, the transfer device 20 for transporting the crushed food waste from the shredder 10 is the shredder 10 Installed at the bottom of the The inside of the conveying device 20 is equipped with a screw 21 for conveying food through the rotational force, the decomposition reaction tank 30 that is connected to the conveying device 20 can accommodate the food waste transported is It is installed.

The decomposition reaction tank 30 is provided with a stirring device 31 for stirring food introduced into the inside, the lower portion is formed with an opening 33 having a predetermined width along the longitudinal direction.

A sliding plate 35 capable of opening and closing the opening 33 is installed at a lower side of the decomposition reaction tank 30, and the sliding plate 35 may be manually opened or closed by an operator. have.

In addition, the discharge means 40 is provided in the lower portion of the decomposition reaction tank 30 in order to automatically discharge the food wastes disintegrated and extinguished in the decomposition reaction tank 30 to the outside.

The discharge means 40 is integrally mounted to the lower portion of the decomposition reaction tank 30 and connected to one end of the housing 45 and the housing 45 in communication with the opening 33 can move freely A discharge pipe 46 made of a flexible material is provided. The first screw 41 is disposed inside the housing 45 for discharging the food wastes in which the first screw 41 is disintegrated and extinguished. The second screw 43 is installed in the discharge pipe 46. The first screw 41 and the second screw 43 are connected by the joint 42.

In addition, the drive motor 44 is installed on one side lower portion of the housing 45 to be connected to the first screw 41, so that the first screw 41 is rotated by the rotation of the drive motor 44. do.

In addition, a cap 47 for opening and closing the opening end of the discharge pipe 46 is installed at the tip of the discharge pipe 46 to prevent odor generated through the discharge pipe after discharging the food waste.

On the other hand, the decomposition reaction tank (30) is provided with a heater (not shown) is heated in the winter or cold waste is introduced when the temperature of the decomposition reaction tank 30 is sharply lowered to heat the decomposition reaction tank (30).

An upper portion of the decomposition reaction tank 30 is provided with an air injection device 60 for introducing air into the decomposition reaction tank 30 and adjusting the temperature of the air introduced therein. In the lower portion of the air injection device 60, a heater unit 67 for heating the air introduced from the air inlet hole 61 is installed to adjust the temperature of the inlet air, the upper air is introduced at a predetermined interval A plurality of blocking plates 62 are formed to guide the flow of air to guide the flow of air, and one side of the thermometer side sensor 65 is installed to detect and control the air temperature in the air injection device 60. That is, the thermometer sensor 65 detects when the temperature of the air in the air injection device 60 is lower than the temperature in the decomposition reaction tank 30 to operate the heater unit 67, where the heater unit 67 ) Heats the blocking plate 62, so the air inlet path itself has the same effect as a heat exchanger. In this case, a separate blower fan 63 is installed inside the air inlet hole 61 to operate by a command of the control unit so that external air can be forcibly introduced into the air injection device 60.

The upper part of the decomposition reaction tank 30 is provided with a filter box 50 for filtering the dust contained in the odor gas (ammonia gas, trimethylamine, methyl mecaptan, hydrogen sulfide, acetic acid), the filter box 50 Although not shown inside, a filter and a humidity sensor are installed. The humidity measurement sensor may adjust the amount of air introduced into the decomposition reaction tank 30 by sensing the humidity of the odor gas exhausted.

Deodorizing means 70 is finally installed inside the main body 2 to finally filter the malodorous gas filtered primarily through the filter box 50 and to burn and remove the dust.

Looking at the configuration of the deodorizing means 70, the primary odor gas filtered from the filter box 50 selectively flows from the intake pipe 73 to the first branch pipe (75) or the second branch pipe (77). This is done by an on / off valve 71 operating by command of the control unit. On the first branch pipe 75, a first deodorizer 80 for filtering and burning the malodorous gas introduced from the first branch pipe 75 is installed, and on the second branch pipe 77, A second deodorizer 80a for filtering and burning the malodorous gas introduced from the second branch pipe 77 is provided. In addition, a ceramic heating wire 90 is provided on the exhaust pipe 79 for re-burning the exhaust gas filtered by the first and second deodorizers 80 and 80a. Exhaust gas that does not can be discharged to the outside through the exhaust pipe (79).

In addition, the first deodorizer 80 is provided with a plurality of activated carbon fibers 81 are spaced apart from each other, the heating coil 83 is installed at a lower portion of each of the activated carbon fibers 81 at a predetermined interval. do. Similarly, the second deodorizer 80a is provided with a plurality of activated carbon fibers 81a spaced apart from each other, and a heating coil 83a is installed at a lower portion of each activated carbon fiber 81a at a predetermined interval. do.

The activated carbon fiber has a fine hole of 5 ~ 10Å, has a specific surface area of 1,000 ~ 2,000 ㎡ / g, the adsorption force is 200 times or more than activated carbon, can be regenerated at a lower temperature than ordinary activated carbon, nonwoven fabric type It is made of high quality and good maintenance.

To explain the operation, first, the cover 4 formed on the outside of the main body 2 is opened and food waste is put into the inlet 4a. The input food waste is crushed and crushed by the crusher 10 and moved to the transfer device 20. Food waste is transferred to the decomposition reaction tank 30 by the rotation of the screw 21 mounted inside the transfer device 20.

The food waste transported to the decomposition reaction tank 30 is agitated by the rotation of the stirring device 31, and the food waste and microorganisms are mixed at this time, so that the decomposition is quickly performed. In addition, the inside of the decomposition reaction tank 30 is to maintain the proper humidity and oxygen supply, which can be achieved by the air injection device (60). That is, when the blowing fan 63 of the air injection device 60 is rotated by a motor (not shown), external air is forcibly introduced through the air inlet hole 61, and the introduced air is introduced into the blocking membrane 62. It is moved along the flow path formed by the supply to the interior of the decomposition reaction tank (30).

At this time, if the thermometer sensor 65 detects the temperature of the inlet air and is lower than the set temperature, it is heated and supplied through the heater unit 67, and the humidity measurement sensor (not shown) built in the filter box 50 is Humidity of the exhaust gas is measured to adjust the amount of air introduced.

During the process of decomposition and extinction of food, the odor gas generated is first filtered through the filter box 50 and moved to the deodorizing means 70 through a transfer pipe (not shown).

The malodorous gas passing through the intake pipe 73 of the deodorizing means 70 flows into the first deodorizer 80 via the first branch pipe 75. At this time, the second branch pipe 77 is closed by the on-off valve 71 so that no odor gas is introduced. The introduced malodorous gas is filtered while passing through a plurality of activated carbon fibers 81, and is filtered for about 6 to 10 hours. Therefore, after the filtration operation is performed for a predetermined time, power is supplied by the control unit, and each heating coil 83 is heated to remove odorous substances such as dust stuck to the activated carbon fibers 81 from 5 to 20. It is regenerated by burning at 350 ~ 500 ℃ for minutes. At this time, the opening / closing valve 71 closes the first branch pipe 75 by the command of the controller, opens the second branch pipe 77 and simultaneously moves the odor gas to the second deodorizer 80a. Done.

The operation of the second deodorizer 80a is the same as the operation of the first deodorizer 80 mentioned above.

In addition, the exhaust gas filtered from the first deodorizer 80 and the second deodorizer 80a may be burned again while passing through the ceramic heating wire 90 to remove the last odorous substance. At this time, the temperature of the ceramic heating wire 90 is suitably about 800 to 1100 ° C. Therefore, the exhaust gas almost free of odor is finally discharged to the outside.

In addition, the food waste, in which all the decomposition and extinction treatment is completed, may be automatically discharged to the outside by the discharge means 40 installed in the lower portion of the decomposition reaction tank (30).

That is, when the sliding plate 35 provided in the lower portion of the decomposition reaction tank 30 is opened, the processed food waste passes through the opening 33 in the lower portion of the decomposition reaction tank 30 and moves to the housing 45. In this state, when the cap 47 blocking the discharge pipe 46 is opened and the driving motor 44 is operated, the first screw 41 rotates by the rotational force and the second screw connected by the joint 42 ( 43) rotates. The food waste is discharged to the outside by the forward rotation of the first screw 41 and the second screw 43. In this case, since the discharge pipe 46 is made of a flexible material and can move freely, the processed food waste can be put in a desired storage container or storage place.

On the other hand, when the drive motor 44 is rotated in reverse, the first screw 41 and the second screw 43 is rotated in reverse to dispose the food waste remaining in the housing 45 and the discharge pipe 46 to the decomposition reaction tank 30 ) To the inside. When the transfer is completed, the sliding plate 35 is closed to prepare for disassembling and extinguishing the food waste to be input again.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the following examples are presented to illustrate the present invention and are not intended to limit the scope of the present invention.

Example 1 Selection of Microbial Strains of the Invention

Strains isolated from the near sea soil of Gwangyang, Jeollanam-do were inoculated once with platinum in NB liquid medium (0.3% broth, 0.5% peptone) and shaken at 37 ° C. for 5 days.

After the pharmacopoeia standards of the culture measured producing ability of amylase and protease, and to identify from among the strains selected a strain that produces amylase and protease horizontal highest station, it was identified as a new Bacillus sp (Bacillus sp.). This is called Bacillus sp GENO-200 ( Bacillus sp . GENO-200), and the strain was deposited on April 30, 2001 to the Korea Microbiological Conservation Center of the Korean spawn association of Korea and deposited with accession number (accession number: KCCM-10,263). ) Was granted.

Identification of the strains was carried out using the general bacterial identification and MIDI methods described in Bergey's manual of determinative bacteriology, Microbiological methods, Manual of methods for general bacteriology, Microbiology a laboratory manual, Bergey's manual of systematic bacteriology and food engineering experiments. .

Bacillus sp GENO-200 ( Bacillus sp . GENO-200) strains of the present invention were identified in the results and culture characteristics are shown in the following table.

Culture Characteristics of Bacillus sp. GENO-200 Strain of the Invention Colony form Indeterminate Colony surface lubricity Colony corners Roughness Colony Evolution protrusion Colony Blur Opacity Colony color Pale cream Colony gloss Polish

The colony morphology on the Nutrient agar plate medium was indefinite, the color was pale creamy, the surface was shiny and smooth, the growth of the cells in the nutrient liquid medium was good, and it did not form pellicles. In addition, precipitation of cells was observed.

Morphological Characteristics of Bacillus sp. GENO-200 Strains of the Invention. shape Bacillus Cell size 1.1 × 3.8 μm Yundong Province + Flagella + Spore formation + Gram dyeing +

As shown in Table 2, the Bacillus sp GENO-200 strain of the present invention is a simple bacilli, the cell size is 1.1 × 3.8 ㎛, motility, shows a positive response to Gram staining, forms an elliptical spore in the center It was.

Physiological Characteristics of Bacillus sp. GENO-200 Strains of the Invention. Growth temperature range 15 to 65 ° C Growth pH range 4 to 10 Growth NaCl Limit <25% Catalase + Oxidase + Urease + Lipase (Twin 80 Hydrolysis) + Galactosidase + Arginine Dehydrolase - Starch hydrolysis + Casein hydrolysis + Cellulose hydrolysis + Esculin hydrolysis - Indole formation - H ₂ S Formation in TSI Agar Media + Levan formation from sucrose + NH ₃ formation from peptone - NH ₃ Formation from Arginine + Gelatin liquefaction + Use of site rate + Methyl red test + Nitrogen reduction + Denitrification + Milk reaction: Coagulation - Milk reaction: peptonization + O-F test Fermentation Tyrosine breakdown - Phenylalanine Deaminase +

* In the above table, + represents positive and-represents negative.

As shown in Table 3, the growth temperature range of the Bacillus sp GENO-200 strain of the present invention is 15 ~ 65 ℃, growth pH range is 4 ~ 10, it is possible to grow up to 25% salt concentration. Catalase positive and starch, casein and cellulose degradability were found.

Example 2 Review of Mass Production Conditions and Waste Treatment Effects of the Strains of the Invention

To a 100 ml Erlenmeyer flask, 20 ml of medium consisting of 4% corn starch, 2% soybean meal, (NH ₄ ) ₂ HPO _4, 1% NaCl, 0.02%, and MgSO ₄ 0.02% was added, After inoculating once, the strain was cultured for 24 hours while maintaining a pH of 6.8 to 7.0, the culture temperature of 37 ℃ as a seed, inoculated by 1.0% (v / v) of the spawn culture medium in 20ml of the medium and shaking culture As a result of measuring the titer of amylase and protease by taking culture medium at intervals of 24 hours, the titer of amylase was 6,000 units / ml and protease was about 4,000 units / ml after 36 hours of culture.

After freeze drying the culture, 2 kg of lyophilized culture medium and 60 kg of sawdust were added to the food waste treatment apparatus described in the detailed description of the present invention and aged at 35 ° C. for 3 hours, and then collected at a restaurant, dehydrated and shredded. 70 kg of waste was added and treated for 1 day.

After 1 day, 70 kg of food waste and 100 g of lyophilized microorganisms and culture medium with skim milk powder (10%) were added together and treated for 30 days. As a result, food waste was 89% reduced.

Example 3 Review of Food Waste Treatment Efficiency by Mixed Strains

The same method as Example 2 was used, but Bacillus sp GENO-200 as a microorganism ( Bacillus sp . GENO-200), Candida Cylindracea ATCC 1483 as a bacterium having excellent lipase generating ability , Thermobifida Fusca ATCC 27730 was used.

Candida Cylindracea ATCC 1483, which has excellent lipase- producing ability , and microbial Thermobifida Fusca ATCC 27730, which has high cellulose degrading capacity , according to the disclosure method. , PH 5, peptone 5g, yeast extract 5g incubated for pH 5.0, incubation temperature 25 ℃, aerobic 36 to 42 hours, thermopida fusca 3.0g tryptophan per liter of distilled water, 50g yeast extract, 3g glucose , K ₂ HPO ₄ 1g incubated at pH 7.4, incubation temperature of 37 ℃, aerobic 48 to 72 hours and used for freeze drying, when the number of viable cells was 10 ⁸ cfu / g 6: 2: 2 The reduction ratio of the food waste was 98% as a result of mixing at a ratio of.

Example 4 Oral Toxicity Test

Specific pathogens free of 7-8 weeks of age, 6 females weighing 25-29 g and 6 males weighing 34-38 g, temperature 22 ± 1 ° C, humidity 55 ± 5%, illumination 12L / 12D Was bred in the animal room. Mice were allowed to acclimate for about a week before being used in the experiment. Feed for experimental animals (Jeil Jedang Co., Ltd., mice and rats) and drinking water were supplied after sterilization and free ingestion.

Culture supernatants of the strains obtained in Example 4 were orally administered 0.2 ml per 20 g of mouse body weight. Samples were administered orally once and observed for side effects or lethality for 10 days after administration. That is, on the day of dosing, changes in general symptoms and the presence or absence of dead animals were observed at least once in the morning, at least 1 pm, 4 hours, 8 hours, 12 hours, and the next day until the 10th day of administration. In addition, the animals were killed and dissected at 10 days of administration, and the internal organs were visually examined. Changes in body weight were measured at daily intervals from the day of administration to observe the weight loss phenomenon of the animals.

This experiment was carried out for the purpose of deriving the basic data on the toxicity by grasping the degree of acute toxicity in the oral route of the culture supernatant of the strain of the present invention was obtained as follows.

In the case of acute oral toxicity, no lethal animals were observed for 10 days at the dose of 1000 mg / kg of the strain culture supernatant of the present invention. An autopsy of the surviving animals was performed 10 days later, and there were no gross lesions, and normal body weight gain was observed without any weight loss for 10 days from the day after the administration.

In conclusion, the strain culture supernatant of the present invention was not observed toxicity upon oral administration at the above concentration.

As described above, in the present invention, the microorganism strain or culture is mixed with wood chips such as sawdust and aged for 1 to 5 hours at 30 to 40 ° C., and then dehydrated and crushed food waste is added thereto. After one night of cultivation and from the next day, the food waste and the microbial strain or culture were added continuously to reduce the food waste for one month, and the remaining amount was reused as organic fertilizers, thereby easily treating the food waste.

Claims (5)

As a simple bacilli, the cell size is about 1.1 × 3.8㎛, motility, shows a positive response to gram staining, elliptical spores are formed in the center, the growth temperature range is 15 ~ 65 ℃, can grow up to 25% salt concentration Bacillus sp GENO-200 ( Bacillus sp . GENO), which has catalase-positive and starch and casein cellulose degradability, and has a titer of amylase of about 6,000 units / ml and a protease titer of about 4,000 units / ml . -200, microorganism accession number: KCCM-10,263) strain. Bacillus sp . GENO-200 ( Bacillus sp . Then, the food waste treatment method characterized in that the culture for one day and the food waste and the microbial strain or culture from the next day is added continuously to reduce the food waste for one month and the remaining amount is reused as organic fertilizer. The method of claim 2, wherein the microorganisms having excellent lipase generating ability and microorganisms having high fibrinolytic ability are used together. The method of claim 2, wherein the wood chips and Bacillus sp . GENO-200 strain or the culture is characterized in that the ratio of wood chips: culture = 20 to 50: 1 based on the culture. How to dispose of garbage. According to claim 3, Candida Cylindracea ATCC 1483 as a microorganism with excellent ability to produce lipase, Thermobifida Fusca ATCC 27730 as a microorganism with excellent fibrinolytic ability is used. How to dispose of food waste.