KR100277347B1 - Novel Lactic Acid Bacteria Lactobacillus Fermentum Kwai 2524 for Feeding Food Waste - Google Patents

Abstract

The present invention is a novel lactobacillus isolated from pig feces, Lactobacillus Permantum KY 2524 for feeding food waste (deposited institution: Korean spawn association, date of deposit: November 18, 1998, accession number: KFCC-11060) and this It relates to a livestock feed used and its manufacturing method. Lactobacillus Permanentum KY 2524 of the present invention exhibits a survival rate of 10% or more when incubated for 180 minutes in an acidic buffer solution of pH 2.1; Oxgall exhibited a survival rate of at least 95% for 120 minutes in 0.15 and 0.30% (w / v) added buffer, respectively; Survive 20 minutes at 70 ° C .; Resistance to Virginia mycin, neoterra (neomycin plus terramycin), ASP (ASP), aureomycin, thiamulin; It is a new lactic acid strain that has excellent acid resistance, bile acid resistance, heat resistance, antibiotic resistance and proliferation ability in food wastes, which grows from about 10 ⁶ to 10 ⁸ cfu when incubated for 15 hours in non-neutralized food wastes. Can be used as a probiotic for the addition of livestock feed, in particular can be useful for the feed of food waste by fermentation.

Liquid livestock feed prepared from food waste using Lactobacillus fermentum KY 2524 will contain lactic acid bacteria above 10 ⁹ cfu per gram of feed, and nutritionally by adding fertilized water and / or molasses to the medium during fermentation. It is possible to produce liquid livestock feed that is more balanced, palatable, and has increased feed efficiency.

Description

Novel Lactic Acid Bacteria Lactobacillus Fermentum Kwai 2524 for Feeding Food Waste

The present invention is a lactobacillus fermentum KY 2524 (Lactobacillus fermentum KY 2524) for depositing food waste with a novel lactic acid bacteria isolated from pig feces (depository institution: Korean spawn association, date of deposit: November 18, 1998, accession number: KFCC-11060) and a livestock feed using the same and a method of manufacturing the same.

Since Korea's food culture is vegetarian-oriented and there are many broth-type foods, the dietary life has been westernized, but the amount of food waste is still higher than in other countries. In addition, most of the food waste is discarded as it is, not recycled regardless of the city or rural areas. In the past, food waste emitted from homes was used as feed for pigs, but nowadays, due to the development of the livestock industry and the enlargement of the breeding unit, it is not possible to breed leftover foods. It is used. Therefore, the share of feed prices in pigs is increasing day by day, and huge amounts of money are being spent abroad for the purchase of feedstock. In 1996, the amount of food waste generated per day was 14,532 tons, which accounted for 29.1% of the total household waste. In terms of money, more than 8 trillion won is wasted annually. 92.8% of the food wastes are landfilled, 3.9% are incinerated and only 3.3% are recycled as compost or feed. Since food waste has high water content and contains organic matters, sewage and odors are generated during collection and transportation, and high levels of leachate are generated when landfilling causes pollution of soil and groundwater, and prevents the cost of prevention. It raises the treatment cost and causes secondary environmental pollution such as bad smell, harmful gas and air pollution caused by corruption. In addition, the high water content lowers the incineration efficiency during waste incineration, thereby increasing the incineration cost such as supplying auxiliary fuel, and generating harmful substances that adversely affect the human body such as hydrogen chloride and dioxins due to the lowering of the incineration temperature. Therefore, there is an urgent need for efficient treatment and recycling of food wastes, and the suggestion of food wastes has been suggested as a preferred method.

At present, food waste is mainly used for pig feed, and as a feed method, it is provided as a raw food or dried, fermented, and wet fermented feed. It is not preferable to use it as a feed for livestock because food waste is easy to rot and there is a possibility of animal disease caused by contamination of pathogenic microorganisms.

On the other hand, the method of drying by feed with high speed hot air and steam by mechanical equipment is convenient to collect and transport by weight loss, and there is no concern about infectious diseases because pathogens are killed by high temperature, but nutrients are not uniform and drying costs are high. There is a problem.

In addition, the wet fermentation method of fermenting organic matter of food waste by adding microorganisms and yeasts to fermentation equipment minimizes the loss of organic and inorganic matters during fermentation and regulates nutritional balance, thus making it possible to produce high-quality feedstock, but at a low cost. It is not easy to supply a sufficient amount of water conditioner, and if the decay microorganisms among aerobic thermophilic microorganisms remain in the fermented product, it may deteriorate and cause odor, and it is unsanitary and the nutritional value of the product is also reduced. do.

In addition, food waste is usually 80% or more water content, the main components are cellulose of vegetables, carbohydrates such as rice, noodles, protein of meat and fish, and fat of Uji, etc. Grain> Fruits. In addition, the nutritional component is very variable depending on the source and season, but the protein is 12-43%, crude fat 8-30%, crude fiber 2-9%, salt concentration up to 4.5%, pH is about 4.7 It is enough. Therefore, most microorganisms do not grow in food waste, and only some pathogenic bacteria and food rot grow in food waste. Therefore, it is difficult to effectively contain microorganisms useful for livestock even in a wet fermentation method in which microorganisms are fermented. .

In addition, the conventional techniques for feeding food waste are mostly concentrated on the technology of the fertilizer method or feed manufacturing equipment, and the method of nutritionally balanced, palatable and increasing feed efficiency using food waste. Things were insignificant or little.

Therefore, the present inventors have studied new microorganisms that can effectively ferment food wastes and feed them and at the same time secure safety and benefits for livestock, and as a result, isolate and identify new lactic acid bacteria from pig feces. .

The present invention is a novel lactic acid bacteria isolated from pig stool, lactobacillus fermentum KY 2524 (depositing institution: Korean spawn association, deposit date: excellent in acid resistance, bile acid resistance, heat resistance and antibiotic resistance, and at the same time excellent proliferation ability in food waste) November 18, 1998, Accession No .: KFCC-11060) and its use.

In addition, an object of the present invention is to provide a livestock feed and a method for producing the same, the food waste feed using Lactobacillus Permanentum KY 2524.

1 is a growth curve of the Lactobacillus permanent KY 2524 in the neutralized food waste,

Figure 2 is a growth curve of Lactobacillus Permanentum KY 2524 in food waste added with immersion water,

3 is a growth curve of Lactobacillus permanent KY 2524 in molasses-added food waste,

4 is a growth curve of Lactobacillus permanent KY 2524 in food waste mixed with immersion water and molasses.

Lactobacillus latement KY 2524 according to the present invention is a novel strain of Lactobacillus latement isolated from the feces of piglets to secure safety and benefits for livestock.

Isolation and Identification of Strains

After collecting the feces of piglets and adjusting it to pH 3.0, anaerobic culture was performed on LBS agar plate medium, a selective medium of Lactobacillus strains, and the colonies shown were purely subcultured in MRS medium. Primary separation with lactobacillus. The isolated strain was allowed to stand for 3 hours in an anaerobic dilution adjusted to pH 2.5 again, and the surviving strains were secondarily selected as a highly acid resistant strain, and for 3 hours in an anaerobic dilution with 0.15% oxgall. After the reaction, the cells are cultured in MRS medium and selected as strains with bile. Among them, after selecting strains having heat resistance, strains resistant to antibiotics mainly used in livestock feed were selected, and finally, strains showing excellent growth ability in food wastes collected on the market were isolated. The isolated lactic acid bacteria were identified through a buggy's manual and carbohydrate digestion experiments, and the isolated strain was identified as a new strain of Lactobacillus fermentum, and the new strain was identified as Lactobacillus fermentum KY 2524. Korea spawn association, date of deposit: November 18, 1998, accession number: KFCC-11060), the strain characteristics are as follows.

Strain characteristics

(1) form of colony

Form of colony when anaerobic incubation at 37 ℃ for 48 hours in MRS agar plate

1) Shape: Round

2) Size: 1-3mm

3) Hue: off-white

4) Surface: Smoothly

(2) form of bacteria

Bacterial Activity of Anaerobic Cultures at 37 ° C for 48 Hours in MRS Agar Plates

1) Cell shape: rod type

2) Gram Dyeing: Positive

3) Mobility: None

4) Spore Formability: None

(3) physiological properties

1) Growth temperature: Growth range: 25 ~ 45 ℃

Optimum Temperature: 35 ~ 40 ℃

2) Growth pH: Growth range: pH 2.0∼8.0

Optimum pH: pH 4.5 ~ 7.0

3) Influence of oxygen: breathable anaerobic

4) Catalase:-

5) Applied Solidification:-

6) Gas Formation: Slightly

7) Beta-galactose degrading enzyme: +

8) alpha-glucose degrading enzyme: +

9) Sugar availability: L-Arabinose: +

Adonitol:-

Galactose: +

D-glucose: +

D-fructose:-

D-Mannose:-

Sorbitol:-

Amigdalin:-

Cellobiose:-

Maltose: +

Lactose: +

Melibiose: +

Saccharose: +

Melezitose:-

Raffinose: +

Inulin:-

Amidodon:-

Genthiobis:-

Gluconate:-

(4) acid resistance

Lactobacillus permanent KY 2524 showed high acid resistance by surviving more than 10% even when incubated for 180 minutes in an acidic buffer solution of pH 2.1.

(5) bile acid resistance

Lactobacillus permanent KY 2524 showed excellent bile acid resistance by surviving more than 95% in oxgall buffer with 0.15 and 0.30% (w / v), respectively, for almost 120 minutes.

(6) heat resistance

100 ml of Lactobacillus Permanentum KY 2524 was inoculated into 2 ml of sterilized base diluent, and heat-treated for a predetermined time, and then plated on an MRS medium to determine heat resistance. It showed high heat resistance.

(7) antibiotic resistance

The resistance of the Lactobacillus fermentum KY 2524 to seven antibiotics (rinsmycin, virginiamycin, neoterra, asp, sulfatiazole, aureomycin, and thiamuline) currently being added to domestic animal feed were measured. . First, the antibiotics were dissolved in 1 g of 10 ml of sterilized distilled water in order to separate the antibiotics and excipients, and then centrifuged at 1500 rpm for 110 minutes, and the supernatant was passed through a 0.45 μm membrane filter to make a stock solution. 1 ml of rinsomycin (Lycommycin + Spectinomycin), 2.5 ml of Viriniamycin, 1 ml of Neomycin (teromycin), Asp (ASP 250: Chlorotetracycline + Snow) per 100 ml in TS agar plate 1 ml of patazole + penicillin), 0.5 ml of sulfatiazole, 1 ml of aureomycin, and 2 ml of thiamulin, respectively, and then lactobacillus fermentum KY 2524 was plated on the medium at 37 ° C. The antibiotic resistance was tested by anaerobic treatment for 48 hours at. Testing showed no resistance to sulfatiazole and rinsomycin, but resistance to all five other antibiotics.

(8) Growth in food waste

The result of cultivation in general food wastes collected on the market showed that Lactic acid bacteria did not grow properly, whereas Lactobacillus Percentum KY 2524 grew from about 10 ⁶ to 10 ⁸ cfu after 15 hours incubation, showing excellent growth ability even in food wastes. It was.

As described above, the Lactobacillus pertumtum KY 2524 of the present invention is a new strain of Lactobacillus pertumtum isolated from pig feces, which has superior acid resistance, bile acid resistance, heat resistance and antibiotics compared to lactic acid bacteria which are conventionally used as livestock probiotics. It is suitable to be used as livestock probiotics due to its resistance, and at the same time, unlike the existing lactic acid bacteria, it shows high proliferative power in food wastes, so it is suitable for food waste foods.

Liquid Feeding Process of Food Waste Using Lactobacillus Fermentum KY 2524

1) Process Ⅰ

Remove the foreign matter from the collected food waste, cut it with a shredder, stir and grind to make porridge, and neutralize it to pH 5.5-6.5 using a neutralizing agent.

Food waste can be used either from home or from a restaurant. However, since food wastes have different characteristics depending on the source or season, it is difficult to secure a feed with a uniform nutritional value. Therefore, it is preferable to collect and use only fresh ones discharged from group meals or large restaurants, rather than households with low emissions. In addition, the perishables can be processed quickly from the point of release to the point of feed to minimize the likelihood that the toxins produced from the debris will remain in the feed.

Among the food wastes, foreign matters that are problematic are vinyl, metals, glass, porcelain debris, toothpicks, detergents, plastics, disinfectants, etc., and these metals are removed from the collected food wastes, and the metals are secondarily selected using magnets. Remove

The sorted food waste is transferred to a stirrer and stirred, and then pulverized in a pulverizer to make porridge.

Agitated food waste is about pH 4.7, which is low for microorganisms to grow, so neutralize it with a pH of 5.5-6.5 using a neutralizing agent such as sodium hydroxide, potassium phosphate, ammonium phosphate or ammonia.

In addition, in order to increase the nutritional value and palatability of the feed to the food waste during the stirring process, 1 to 30% of the immersion water generated as a by-product in the production of corn starch is added, and / or from sugar cane to sugar by-products. Extra molasses can be added to the dietary value of about 1 to 20%. At this time, the added immersion water may provide a carbon source or nitrogen source necessary for the growth of lactic acid bacteria with a crude protein content of 40% or more, and molasses may improve the flavor of food waste livestock feed to brix 80 or more.

2) Process II

Sterilize the food waste in Process I.

Sterilization is usually performed for 1 to 5 minutes at a high temperature of 150 ~ 160 ℃ to sterilize pathogens and other various bacteria present in food waste.

3) Process III

Liquid livestock feed is prepared by inoculating and fermenting Lactobacillus percent KY 2524 into the food waste of step II.

After cooling the temperature of the sterilized food waste in Step II to about 30-40 ° C., inoculated therein with about 10 ^{6-10 7} cfu per gram of food waste, and about 16-24 hours. It is fermented and aged while stirring. At this time, Lactobacillus fermentum KY 2524 releases an enzyme that breaks down nutrients as it proliferates, so starch and carbohydrates derived from grains in food waste are broken down into glucose, proteins are broken down into amino acids, and volatile lactic acid bacteria produce. Organic acids and lactic acid reduce odors and immunity from food waste.

In addition, the feed may be prepared by adding an appropriate amount of additive feed including starch sweet food, protein sweet food, fat sweet food, other minerals, vitamins, flavors, and the like, or may be added to feed animals.

In addition, the liquid feed may be dried as needed to form a dry feed.

Usage

Lactobacillus Fermentum KY 2524, a new lactic acid strain isolated from pig feces, has excellent acid resistance, bile acid resistance, heat resistance and antibiotic resistance as well as useful for activation of enterobacterial flora of common lactic acid bacteria. It can be used as a probiotic for the addition of livestock feed with functions of prevention of mortality, improvement of milk fat, breeding efficiency, feed efficiency and growth promotion.

In addition, by using the Lactobacillus Permanentum KY 2524 of the present invention by fermenting the food waste can be effectively recycled food waste discharged to the waste, the liquid feed of the present invention prepared in this way the lactic acid bacteria 10 g per feed ^Since it contains ⁹ cfu or more, it contains about 10-100 times more microorganisms than the concentration of microorganisms contained in the probiotic added to livestock feed. Therefore, the feed of the present invention does not need to add a separate probiotic, and because it is obtained in a liquid state, the vitality of the lactic acid bacteria is also stronger than that of the dry probiotic, and the feed during storage or transportation due to the contained lactic acid bacteria and lactic acid bacteria culture Is less likely to be contaminated by decaying bacteria and pathogenic bacteria.

Hereinafter, the present invention will be described in more detail with reference to Examples. However, the scope of the present invention is not limited by the following examples, and ordinary changes by those skilled in the art are possible within the scope of the technical idea of the present invention.

Example 1

Isolation and Identification of Lactobacillus Permanentum KY 2524

Piglets were collected and homogenized in an anaerobic diluent (1 L, 4.5 g of potassium phosphate, 6.0 g of sodium phosphate, 0.5 g of L-cysteine, 0.5 g of Tween-80, 1.0 g of Bactoagar), followed by pH of the stock solution with concentrated hydrochloric acid. Adjust to 3.0 and stir for 1 hour. The plate was then plated on LBS agar plate medium, which is a selective medium for Lactobacillus strains, and anaerobic culture was performed at 37 ° C. for 48 hours. The colonies shown at this time were purely subcultured in MRS medium for 48 hours, and then separated into Lactobacillus having acid resistance through gram staining and microscopic observation. The isolated strains were allowed to stand in an anaerobic dilution adjusted to pH 2.5 again for 3 hours, and the surviving strains were secondarily selected as highly acid resistant strains. In addition, after reacting for 3 hours in an anaerobic dilution solution containing 0.15% oxal, the strains grown in MRS medium were selected as bile strains, and among these strains, they survived after 20 minutes at 70 ° C. Strains were selected and again strains with excellent resistance to antibiotics were selected. Antibiotics include rinsmycin (Rincomycin + Spectinomycin), Virginiamycin and Neoterra, which are currently added to domestic feed.

7 antibiotics were used (neomycin + teramycin), ASP (ASP 250: chlorotetracycline + sulfatiazole + penicillin), sulfatiazole, aureomycin and thiamulin. Finally, isolates having excellent growth ability from food wastes collected on the market were identified through the Burgy's manual and carbohydrate digestion experiments, and named as Lactobacillus permanent KY 2524.

Example 2

Culture in food waste

Commercially-collected food waste was 50g measured as it was, without adjusting pH, and sterilized at 121 ° C for 15 minutes. The sterilized food waste was inoculated with 1% each of four kinds of lactic acid bacteria including Lactobacillus latement KY 2524, and the number of bacteria was measured every 3 hours while incubating at 37 ° C for 15 hours. The bacterial count was measured every 3 hours by taking 1 ml of the food waste culture solution and diluting it in dilute physiological saline solution, spreading the diluted solution on MRS or M17 agar plate medium and incubating for 48 hours at 37 ° C. The numbers were counted and compared. The results are shown in Table 1 below.

Table 1

Fungal species Viable Count (cfu / mL) 0 hours 3 hours 6 hours 9 hours 12 hours 15 hours KY 2524 5.5 × 10 ⁶ 1.0 × 10 ⁷ 5.2 × 10 ⁷ 1.4 × 10 ⁸ 2.3 × 10 ⁸ 2.7 × 10 ⁸ Lactobacillus B 2.4 × 10 ⁶ 3.0 × 10 ⁷ 4.7 × 10 ⁷ 1.7 × 10 ⁸ 1.7 × 10 ⁸ 4.5 × 10 ⁷ Lactobacillus A 1.5 × 10 ⁷ 1.5 × 10 ⁷ 1.7 × 10 ⁷ 5.4 × 10 ⁷ 4.8 × 10 ⁷ 4.4 × 10 ⁷ Lactobacillus B 2.5 × 10 ⁷ 2.6 × 10 ⁷ 3.3 × 10 ⁷ 3.2 × 10 ⁷ 3.1 × 10 ⁷ 3.1 × 10 ⁷

cfu: colony forming unit

As can be seen from Table 1, Lactobacillus Percentum KY 2524, unlike other lactic acid bacteria, showed a high proliferation rate of 2.7 × 10 ⁸ cfu even in non-neutralized food waste medium, and did not die after 15 hours of incubation. The bacterial count was maintained. In general, food waste is not a suitable medium for the growth of lactic acid bacteria. Therefore, in order for lactic acid bacteria to grow normally, it is necessary to secrete abundant enzymes capable of breaking down nutrients in food waste. It seems to have this ability to grow in the world.

Example 3

Culture in Neutralized Food Waste

Lactobacillus fermentum KY 2524 was inoculated in a 10 ml MRS liquid medium in a certain amount and incubated overnight at 37 ℃. Neutralize the same food waste as Example 2 collected on the market with sodium hydroxide to pH 6, measure 50 g, sterilize at 121 ° C. for 15 minutes, and inoculate 1% of the culture solution into sterilized food waste medium at 37 ° C. The culture was measured every 3 hours while incubating for 21 hours. For the measurement of the number of bacteria, take 1 ml of the food waste culture solution every 3 hours, dilute in dilute physiological saline solution, spread the diluted solution on MRS agar plate medium, incubate for 48 hours at 37 ℃, and then display the colony number. Counting, and the results are shown graphically in FIG.

As a result of the culture, Lactobacillus permanent KY 2524 was grown at a concentration of 7.8 × 10 ⁸ cfu / ml in the neutralized food waste medium, which was about 3 times higher than the non-neutralized food waste.

Example 4

Cultivation in Food Waste Added with Jade Water

Neutralize the same food waste as Example 2 collected on the market with sodium hydroxide to pH 6, add immersion water produced as a by-product when producing corn starch at 20% concentration, and weigh 50 g to sterilize at 121 ° C. for 15 minutes. It was. Sterile food waste medium was inoculated with 1% of Lactobacillus permanent KY 2524 incubated overnight in a 10 ml MRS liquid medium, and the number of bacteria was measured every 3 hours while incubating at 37 ° C. for 21 hours. The method of measuring the number of bacteria was the same as in Example 3, and the results are shown graphically in FIG.

As a result of the culture, Lactobacillus permanent KY 2524 was grown to a maximum of 1.2 × 10 ⁹ cfu / g, showing a high growth rate compared to the medium of Example 3 without the addition of immersion water. In addition, the concentration of lactic acid bacteria is about 10 to 100 times higher than the concentration of the microbial preparation for livestock, which indicates that the feed fermented food waste of the present invention can also be used as a livestock probiotic.

Example 5

Cultivation in Food Waste Added with Molasses

Neutralize the same food waste as Example 2 collected on the market with sodium hydroxide to pH 6, add molasses produced as a by-product from sugar cane to a concentration of 10%, and then weigh 50 g at 121 ° C. for 15 minutes. Sterilized. Sterile food waste medium was inoculated with 1% of Lactobacillus permanent KY 2524 incubated overnight in a 10 ml MRS liquid medium, and the number of bacteria was measured every 3 hours while incubating at 37 ° C. for 21 hours. The measurement method of the number of bacteria was the same as in Example 3, the results are shown graphically in FIG.

As a result of the culture, Lactobacillus permanent KY 2524 was grown to a maximum of 9.65 × 10 ⁸ cfu / g in molasses-containing food waste medium, showing a higher growth rate than the medium of Example 3 without adding molasses.

Example 6

Cultivation in Food Waste Added with Jade Water and Molasses

Neutralize the same food waste as Example 2 collected on the market with sodium hydroxide to pH 6, mix and add ammonia and molasses at 20% and 10% concentrations, respectively, and weigh 50g to sterilize at 121 ° C for 15 minutes. It was. Sterile food waste medium was inoculated with 1% of Lactobacillus permanent KY 2524 incubated overnight in a 10 ml MRS liquid medium, and the number of bacteria was measured every 3 hours while incubating at 37 ° C. for 21 hours. The measurement method of the number of bacteria was the same as in Example 3, the results are shown in the graph in FIG.

As a result of the culture, Lactobacillus Permantum KY 2524 was grown to 1.5 × 10 ⁹ cfu / g or more in a medium containing mixed water of immersion and molasses in neutralized food waste, and cultured in the neutralized food waste of Example 3. Of course, the growth rate was also higher than that of Example 4 and Example 5, in which only molasses and immersion water were added, respectively. Therefore, it was determined that the culture of Lactobacillus fermentum KY 2524 in food waste added with immersion water and molasses could maintain a high bacterial count for a certain period of time during the storage of feed.

Example 7

Culture on Food Waste According to Lactic Acid Bacteria Inoculation Concentration

Neutralize the same food waste as Example 2 collected on the market with sodium hydroxide to pH 6, mix and add ammonia and molasses at 20% and 10% concentrations, respectively, and weigh 50g to sterilize at 121 ° C for 15 minutes. It was. In sterile food waste medium, Lactobacillus permanent KY 2524 incubated overnight in 10 ml MRS liquid medium for 10 g each³, 10⁴, 10⁵, 10⁶, 10⁷Cfu was inoculated, and the number of bacteria was measured every 3 hours while incubating at 37 ° C. for 21 hours, and the measurement method was the same as in Example 3. As a result of measurement, initial inoculation concentration of Lactobacillus permanent KY 2524 is 10³To 10⁵cfu / g When you do about 10⁸cfu Only grew about 10⁶To 10⁷10 when the initial inoculation of cfu / g⁹cfu It grew more than this.

As can be seen from the above examples, the Lactobacillus permanent KY 2524 according to the present invention is a strain having excellent acid resistance, bile acid resistance, heat resistance and antibiotic resistance in addition to the usefulness of general lactic acid bacteria, and is used as a probiotic for adding livestock feed. In particular, since it shows a high proliferative force in food waste, it can be usefully used for the feed of food waste by fermentation.

In addition, the liquid feed prepared from food waste using the Lactobacillus Permanentum KY 2524 of the present invention contains the lactic acid bacteria above 10 ⁹ cfu per gram of feed, so a large amount of lactic acid bacteria effective in animals without the need to add a separate probiotic. As the liquid is obtained, lactic acid bacteria are more vigorous than the dry probiotics, and the feed can be fed to the pigs by a pressure pump, and due to the contained lactic acid bacteria and lactic acid bacteria cultures, the feed is decayed during storage or transportation. The possibility of contamination by pathogenic bacteria or the like is reduced. In addition, when fermentation of food waste by Lactobacillus fermentum KY 2524 is added to the medium of fertilized water and / or molasses, it is possible to obtain a more nutritionally balanced, palatability and increased feed efficiency.

Claims (7)

Lactobacillus isolated from pig feces, Lactobacillus fermentum KY 2524, characterized by excellent acid and bile acid resistance, heat resistance, antibiotic resistance and excellent proliferation in food waste. , Deposit date: 18 November 1998, accession number: KFCC-11060). 1) Survival rate of more than 10% when incubated for 180 minutes in acidic buffer solution of pH 2.1 2) The survival rate of more than 95% in 120 minutes in the oxgall (0.15, 0.30% (w / v) added buffer solution, respectively) 3) Survival after 20 minutes at 70 ℃ 4) Resistant to Virginiamycin, Neoterra (Neomycin + Terramycin), ASP, Aureomycin, and Thiamulin 5) Proliferation from about 10 ⁶ to 10 ⁸ cfu when incubated for 15 hours in non-neutralized food waste Probiotics for livestock feed, characterized in that it contains the cells of Lactobacillus Permanentum KY 2524 (Deposit Organization: Korean spawn association, Deposit Date: November 18, 1998, Accession No .: KFCC-11060). Method for producing livestock feed, characterized in that fermentation of food wastes using Lactobacillus Permanentum KY 2524 (depository institution: Korean spawn association, deposit date: November 18, 1998, accession number: KFCC-11060) as a spawn. . The method according to claim 3, wherein fermentation is carried out by adding immersion water, molasses or a mixture thereof to the food waste. The method according to claim 3, wherein the food waste is fermented after adjusting to pH 5.5 to 6.5. The method of claim 3, (a) adding 1-30% of immersion water and 1-20% of molasses to the food waste; (b) neutralizing the mixture obtained in (a) to pH 5.5-6.5; (C) The production method characterized in that the inoculated at a pH neutralized mixture obtained in (b) the Lactobacillus Percentum KY 2524 at an initial inoculation concentration of 10 ⁶ ~ 10 ⁷ cfu / g and fermentation. Livestock feed, characterized in that produced by the method of any one of claims 3 to 6.