MXPA06003777A - Adapted microorganism compound for biodegrading the organic fraction contained in solid residues, and process for the preparation thereof. - Google Patents

Abstract

The present invention provides an adapted microorganism compound for biodegrading the organic fraction contained in solid residues. The genetic, metabolic and morphologic structure of said adapted microorganisms effectively works mineralising the biodegradable solid residues resulting from crop and municipal solid wastes, thus reducing the gas and lixiviate production. Said compound enriches and increases the concentration of beneficial microorganisms, thereby generating high quality biological manures used in the agricultural production, the recovery and preservation of soils under parameters established in the sustainable organic agriculture wherein it is intended to preserve, recover and use nature or environment without generating any negative impact thereto.

Description

COMPOSED OF ADAPTED MICROORGANISMS FOR BIODEGRADING ORGANIC FRACTION CONTAINED IN SOLID WASTE AND PROCESS FOR YOUR PREPARATION BACKGROUND OF THE INVENTION The problems of environmental pollution that afflict the large cities of the planet require prompt and efficient solutions that allow improving the quality of life of people and guarantee the conservation and recovery of natural resources.

This research emerges as a need in the search for solutions or alternatives to deal with the serious problem of environmental pollution such as that contributed by the municipal solid waste generated in the different activities of human development.

Initially, the large quantities of garbage and the high percentage of organic matter contained in these wastes were observed and analyzed, which are produced daily in the cities and municipalities, their collection, handling and final disposal; and the inconveniences and damages that arise in the societies due to the lack of responsibility or environmental and social conscience.

The problem of municipal solid waste begins with excessive production, lack of separation at the source, poor disposition, lack of areas for management and the rate of treatment or use.

Among the most important problems that are caused, is the production of polluting gases, which affect the atmosphere, the leached pollutants of soils, groundwater and surface waters and also the generation of disease centers or vectors that transmit them.

To reduce this problem that basically occurs in the use of so-called sanitary landfills and landfills, there is conventional composting, which emerges as an alternative to this problem and is carried out using organic matter from crop residues, municipal solid waste and waste. of slaughter plants.

The compost has an anaerobic section that compromises the majority of its volume producing acid reactions that generate several types of gases, its humidity oscillates in an average of 70% and with a pH that varies between 2 to 10. The excess of humidity that occurs it diminishes the aeration and interferes with self-combustion, due to the heat capacity of the water.

With these conditions, the proliferation of microorganisms such as Clostridium, Pseudomonas, Licheniformes, Streptomyces vulgaris, etc. , whose activity is developed by obtaining energy from sulphated components emitting these gases into the atmosphere.

Likewise, regarding the Carbon - Nitrogen ratio (C / N), the optimum range in organic waste for a proper composting is between 20 and 50 to 1. The excesses of either of the two components lead to a deficiency situation. If the starting residue is rich in carbon and poor in nitrogen, biodegradation will be slow, temperatures will not be high and carbon will be lost in the form of carbon dioxide. Otherwise, in high relative concentrations of nitrogen, it will be transformed in ammonia, preventing the correct biological activity and generating the production of odorous gases.

Under certain conditions some gases are produced, which represent 89% of the volume produced by biochemical reactions, within this type of gases are: • c¾ Methane Gas (60 - 70%) • H2S Hydrochloric Acid (2 - 5%) • CO - Carbon Monoxide (2 - 5%) • co2 Carbon Dioxide (26 - 30%) • ¾ and N2 - Hydrogen and Nitrogen in smaller proportion.

With the humidity that is handled in traditional composting, high levels of leaching are present that contaminate soils, surface waters and groundwater. The leachate is a product that has a Biochemical Oxygen Demand (BOD) that ranges between 20,000 and 80,000 molecules.

The compost generated has a large amount of microorganisms and its effect has not been as expected, there are risks to apply this biostimulator in soils used for pastures or forage crops due to the presence of a certain type of pathogenic bacteria for both plants and animals.

The invention presented arises as an alternative to solve the problems described above because it controls the pathogenic microorganisms present in the process of biodegradation in composting and strengthens the proliferation of beneficial microorganisms such as, the nitrificantés that provide nitrogen required for plant and other saprophyte reproduction, accelerating biodegradation and stabilization.

This invention starts from the observation of conventional compost and after an analysis of the process, enrichment, identification and characterization of the interacting microorganisms was carried out, making a selection of those that presented the best metabolic activity and importance in the different stages of the process. . Thus, the selected microorganisms were subjected to variations of their original environment and medium, seeking a suitable change, adaptation and / or conversion to optimize factors such as time, capacity, speed of biodegradation and product obtained.

This induced adaptation is a response of the mechanisms of microbial resistance that allow a few to withstand, develop and proliferate despite their contact with adverse environments, generating in the term of a few hours fully efficient microorganisms in the specific task of organic biodegradation and under the required conditions .

To obtain the mixture object of the present invention, an adaptation of the microorganisms is carried out, in search of better activity in the biodegradation and mineralization. This adaptation was made by manipulating the physicochemical variables of time, pH, temperature, luminosity, concentration of organic material, organochlorines, organophosphates and mercurials. In the end, a suitable mixture was obtained for the treatment and biodegradation of organic solid waste and the subsequent production of a biological fertilizer with high contents of Nitrogen, Phosphorus, Potassium and minor elements.

During the whole investigation, with each one of the bacteria and in each of the stages were carried out: 1. Modifications to the initial medium. 2. Gram and green malachite colorations for differentiation and observation of cell structures, 3. Growth curves and colony forming unit count per milliliter (CFU / ml) at the times determined for this investigation.

The strains were subjected to direct illumination (visible light) in each of the stages, seeking to contribute to the adaptation and expected modification.

The organic waste that was added to the medium consisted of 80% carbohydrates.

MATERIALS AND METHODS SAMPLING For the taking of samples, cylindrical samples were used to obtain them from a certain depth, then they were transported in polyethylene bags. Transporting the samples to the laboratory under conditions that avoid excess heat and that met the parameters of sterility and thermal insulation. Each sample consisted of 500 g of material in biodegradation. The number of places for sampling was 10 different sites where decaying organic matter was present. From each one, a sample was taken in duplicate at different stages of the decomposition process. The Microcosm Model was used for the isolation of microorganisms (Kabir et al., 1995). The strains were classified by traditional methods (numerical taxonomy) and immunochemical (indirect immunofluorescence-IFI, according to Lle ot and Stead, 1991). Pure strains from different places were used. The sampling places were: 1. Stuffed Doña Juana. 2. Garbage dump El Cortijo. 3. Rubbish dump of Mondoñedo. . Waste Market Square. 5. Residues of Farms. 6. Slaughterhouse waste 7. Domestic waste, The time (time) of sampling was a: A. 2 hours of unloaded B. 1 day of unloaded C. 2 days of unloaded D. 4 days of unloaded E. 12 days of unloaded F. 20 days of unloaded and buried G. 30 days of unloaded and buried H. 40 days of unloaded and buried I. 60 days of unloaded and buried J. 70 days of unloaded and buried From the collected organic material taken to the laboratory, the work of enrichment, isolation and identification of the different species that were in the decomposition process began.

The state of the samples and the processes presented similarities in factors such as pH, odor, color, high humidity, gas generation and leachate in relation to decomposition time.

Each sample of the material acquired, was completely identified, with place of origin, date, time and stage of sampling, organoleptic characteristics and responsible for the intake.

ENRICHMENT, ISOLATION AND IDENTIFICATION MICROORGANISMS The beginning of the work with the sample in the laboratory did not exceed two hours after the taking. We performed a pre-enrichment, isolation and finally the macroscopic, microscopic and biochemical identification of the microorganisms by means of standardized methods and techniques for a specific purpose. This determination was made in the mesophilic and thermophilic stage and in aerobic conditions as anaerobic, in the different processes.

During 12 years of studies and observations with the species of microorganisms present in the process, it was determined that only 10 species of microorganisms were the most functionally relevant, in addition they developed and exercised a much more efficient and metabolically optimal biodegradable and controlling role in the process from biodegradation, in addition to the speed of growth, nutritional behavior, development and biodegradable activity.

These 10 species of microorganisms were induced a change in their genetic and morphological structure, due to the continuous exposure direct illumination (visible light) and different conditions where a certain factor or nutritive constituent was increased or decreased, generating a change and adaptation that It allowed to work efficiently, limiting the time, temperature, pH, humidity and concentrations of organic materials and heavy metals, simulating the possible components of the contaminated organic waste, coming from municipal solid waste (garbage). Finally, the product obtained by inoculating these organisms into a biodegradation process was a biological fertilizer of excellent quality, with high contents of nitrogen, phosphorus and potassium (NPK) and with outstanding physicochemical and microbiological characteristics to be used in sustainable organic agriculture.

PROCEDURE PERFORMED Microorganisms are planted in liquid medium and solid medium simultaneously in each stage and with different modifications in terms of pH, temperature, humidity addition of organochlorines and organic material in proportions that induce an adaptation - selection by means of resistance and metabolic utilization, with direct radiation. In the end, they were evaluated to determine significant differences in: 1. Growth rate 2. Percentage of Inhibition measured in Number of CFU / ml All the tests of thermal stability and pH for all strains showed that the bacterial species withstood the stress to which they were subjected, achieving an initially low activity until achieving variable and high activity, in temperature ranges well above the standard of its habitat. As for the stability tests, their efficient activity at neutral pHs was determined and finally the stress was managed for the microorganisms that changed their condition from strict anaerobic to aerobic, working in each of the tests with permanent forced aeration or percentage oxygen injection.

Initially the microorganisms were planted in triplicate with incubation temperatures defined to later perform microbiological count according to standards of "microbial growth assessment.

The tests were carried out for each species, analyzing the times required for the total coverage of the field of the box. Once this time was determined, it was left to rest for a period of 3 to 5 days and then it was submitted to the next change that had been programmed for each of the investigations.

ADAPTATION OF SELECTED SPECIES Bacillus licheniformis It is a Gram positive, non-pathogenic, facultatively anaerobic, spore-forming bacillus, its initial size is 1.2 μp? wide x 3.5 μ long in average, its optimum temperature of growth oscillates between 10 to 25 ° C and its activity develops within a pH of 5.5 - 6.5, with a humidity from 20 - 40%. The spores that it produces allow it to resist hostile environments, either by heat or drought.

The culture medium used for 1icheniformis is a nutrient medium, consisting of peptone, distilled water, yeast extract and cellulose agar.

STAGES PERFORMED IN THE LABORATORY E-1 On day 1, the culture medium is incubated at 25 ° C and constant oxygen is injected, keeping the other parameters of the primitive culture medium constant. With this change, a mortality of 5% was registered.

E-2 On day 9 the strains are reseeded modifying the culture medium, increasing the temperature range from 25 ° C to 30 ° C, with this change a mortality of 12% is registered.

E-3 On day 21 the microorganisms obtained were reseeded, maintaining the same parameters of the culture medium, adding traces of organochlorines, showing a mortality of 38% microorganisms.

E-4 On day 48, 10% organic waste was added to the culture medium of the microorganisms obtained, this change has a mortality rate of 25%.

E-5 On day 81 the microorganisms obtained are reseeded, maintaining the same conditions until this stage, a second change is made at a temperature of 30 ° C to 35 ° C. There was a mortality of 25%.

E-6 On day 101 the adapted microorganisms were subjected to a change, adding traces of organophosphates to the culture medium, showing a mortality of the microorganisms of 32%.

E-7 On day 126 the obtained strain was subjected to a change in the culture medium, adding 15% of dry and crushed organic material, the other parameters are maintained. In this stage, mortality of 25% of the population was presented.

E-8 On day 146 the strains were reseeded modifying the culture medium, altering the temperature from 35 ° C to 40 ° C with this change a mortality of 25% is registered.

E-9 On day 166 the adapted microorganisms undergo a change adding mercurial traces, presenting a mortality of microorganisms of 30%.

E-10 On day 191 to the culture medium of the microorganisms was added 20% of dry and crushed organic material, keeping the other parameters. In this stage there was a mortality of 18% of the population. E-ll On day 203 the strains were reseeded modifying the culture medium, altering the temperature from 40 ° C to 45 ° C with this change a mortality of 22% is registered.

E-12 On day 220 the microorganisms underwent a change in the culture medium, increasing in 10% traces of organochlorines, maintaining the other parameters. In this stage, a mortality of 27% was presented.

E-13 On day 243, 25% organic waste was added to the culture medium, maintaining the other parameters, with a 15% mortality rate.

E-14 On day 260 the strains were reseeded modifying the culture medium, altering the temperature from 45 ° C to 50 ° C with this change a mortality of 20% is registered.

E-15 On day 283 the microorganisms are subjected to a change in the culture medium, increasing in 10% the traces of organophosphates, maintaining the other parameters. In this stage, a mortality of 25% is presented.

E-16 On day 312 30% organic waste was added to the culture medium maintaining the other parameters, with a mortality of 10%.

E-17 On day 325 the strains are reseeded modifying the culture medium, altering the temperature from 50 ° C to 55 ° C; With this change, mortality of 8% was registered.

E-18 On day 343 with the adapted microorganisms, mercurial traces are increased in the culture medium in a 10%; the other parameters are maintained, with a mortality of the microorganisms of 20%.

E-19 On day 366 the adapted microorganisms underwent a change in the culture medium, increasing organic waste by 40%, maintaining the other parameters, registering a mortality of 10%.

E-20 On day 394 the strains are reseeded by modifying the temperature from 55 ° C to 60 ° C, the other parameters of the culture medium are maintained, this change registers a mortality of 5%.

E-21 On day 419 traces of organochlorines are increased by 15% to the culture medium of the adapted microorganisms, the other parameters are maintained, showing a mortality of 10%.

E-22 On day 436 the culture medium of the obtained strain is subjected to a change adding a 50% concentration of dry and crushed organic material is also subjected to a 5% decrease in humidity from 40% to 35%, keeping the other parameters. In this trial, a mortality of 12% is presented.

E-23 On day 451 the strains are reseeded by modifying the temperature from 60 ° C to 65 ° C, the other parameters of the culture medium are maintained, and the oxygen injection is constant, this change registers a mortality of 5%.

E-24 On day 466 to the culture medium of the adapted microorganisms, traces of mercurial are increased by 10%, the other parameters are maintained, showing a mortality of 8%.

E-25 Day 481 is subjected to a change adding a 55% concentration of dry and crushed organic material to the culture medium of the obtained strain, keeping the other parameters. In this trial a mortality of 6% is presented.

E-26 On day 501 the strains are reseeded by modifying the temperature from 65 ° C to 70 ° C, the other parameters of the culture medium are maintained, this change registers a mortality of 12%.

E-27 On day 510 organ traces of phosphorus are increased by 10% to the culture medium of the adapted microorganisms, the other parameters are maintained, showing a mortality of 8%.

E-28 Day 524 is subjected to a change adding a 60% concentration of dry and stabilized organic material to the culture medium of the obtained strain, maintaining the other parameters. In this trial a mortality of 5% is presented during the process.

E-29 On day 534 the strains are reseeded by modifying the temperature from 70 ° C to 75 ° C, the other parameters of the culture medium are maintained, this change registers a mortality of 5%.

E-30 On day 542 to the culture medium of the adapted microorganisms, organ traces of phosphorus are increased by 10%, the other parameters are maintained, showing a mortality of 8%.

E-31 Day 552 is subjected to a change to the culture medium of the strain obtained by adding a 60% concentration of organic material dry and stabilized, keeping the other parameters. In this trial, a mortality of 3% is presented during the process.

RESULTS OBTAINED At the end of the adaptation process, the medium and the cultivation characteristics were the following: Comparative Table No.l The microorganism presents a normal activity in these conditions, being in its membranes traces of Nitrogen.

It is also observed that the organic material constituted in 80% of carbohydrates stabilizes in a period of 35 days on average, unlike the wild microorganism with which the time was 120 days.

BASILLUS lícheniformís - GRAMPOSITIVO - AEROBIOS or ANAEROBIOS OPTIONAL Colony counts are performed every 36 hours - Gram green colorations of mala uita Clostridivm pasteuranium These microorganisms are Gram-positive, anaerobic and heterotrophic bacilli, their natural habitat is soil, they lack a cytochrome system and a mechanism for phosphorylation with electron transport, and hence they obtain their ATP only by phosphorylation at the level of substrate.

Its optimal development occurs in a range of 20 to 30 ° C of temperature. They are mobile, they move using a flagellum. They are chemoorganotrophic. This bacillus is facultative anaerobic.

STAGES PERFORMED IN THE LABORATORY E-1 On day 1 the strains are reseeded modifying the culture medium, injecting forced aeration and increasing the pH from 5.0 to 5.5%, with this change a mortality of 5% is registered.

E-2 On day 9 the strains are reseeded modifying the culture medium, injecting forced aeration and increasing the temperature from 35% to 40%, with this change \ registers a mortality of 12%.

E-3 On day 22, the microorganisms obtained were reseeded, maintaining the forced aeration and the same parameters of the culture broth, adding traces of organochlorines, showing a mortality of 22%.

On day 43 the same parameters of the culture medium are maintained, and in addition 10% of pulverized and sterilized organic waste is added, this change presents a mortality of 15% of the microorganisms.

On day 58 the microorganisms obtained are subjected to a pH change increasing from 5.5 to 6.0. A mortality of 5% was registered, the total coverage of the field was given after 5 days.

E-6 On day 73 the strains are reseeded modifying the culture medium, injecting forced aeration and increasing the temperature from 40% to 45%, with this change a mortality of 5% is registered.

E-7 Day 83 is subjected to microorganisms adapted to a change, adding mercurial traces, presenting a mortality of microorganisms of 18%.

E-8 On day 103 the same parameters of the culture medium are maintained and in addition 15% of pulverized and sterilized organic waste is added, this change presents a mortality of 12% of the microorganisms.

E-9 On day 123, the microorganisms obtained are subjected to a pH change increasing from 6.0 to 6.5. A mortality of 10% was registered, the total coverage of the field was given after 5 days.

E-10 On day 135 the strains are reseeded modifying the culture medium, injecting forced aeration and increasing the temperature from 45% to 50%, with this change a mortality of 12% is registered.

E-ll The 150th day is subjected to the microorganisms adapted to a change, adding mercurial traces, showing a mortality of the microorganisms of 20%.

E-12 On day 170 the same parameters of the culture medium are maintained and in addition 15% of pulverized and sterilized organic waste is added, this change presents a mortality of 12% of the microorganisms.

E-13 On day 183, the microorganisms obtained are subjected to a pH change increasing from 6.5 to 7.0. A mortality of 10% was recorded.

E-14 On day 200 the strains are reseeded modifying the culture medium, injecting forced aeration and increasing the temperature from 50% to 55%, with this change a mortality of 18% is registered.

E-15 The day 220 is subjected to microorganisms adapted to a change, adding traces of organophosphates, presenting a mortality of microorganisms of 15%.

E-16 On day 245 the microorganisms obtained are subjected to an increase of stabilized organic waste and maintaining forced aeration, incubated at 55 ° C, registering a mortality of 8%.

E-17 On day 257, the microorganisms obtained are subjected to a pH change, increasing from 7.0 to 7.5. A mortality of 10% was recorded.

E-18 On day 274 the strains are reseeded modifying the culture medium, injecting forced aeration and increasing the temperature from 55% to 60%, with this change a mortality of 10% is registered.

E-19 Day 294 is subjected to microorganisms adapted to a change, adding traces of organochlorines, showing a mortality of microorganisms of 15%.

E-20 On day 317 the microorganisms obtained are subjected to an increase in stabilized organic waste and maintaining forced aeration, incubated at 60 ° C, registering a mortality of 12%.

E-21 On day 367 the strains are reseeded modifying the culture medium, injecting forced aeration and increasing the temperature of 60% to 65%, with this change a mortality of 10% is recorded.

E-22 Day 354 is subjected to microorganisms adapted to a change, adding traces of mercurial, showing a mortality of microorganisms of 8%.

E-23 On day 369 the microorganisms obtained are subjected to an increase in stabilized organic waste and maintaining forced aeration, incubated at 65 ° C, registering a mortality of 5%.

E-24 On day 382 the strains are reseeded modifying the culture medium, injecting forced aeration and increasing the temperature from 65% to 70%, with this change a mortality of 8% is registered.

E-25 The day 392 is subjected to microorganisms adapted to a change, adding traces of mercurial, showing a mortality of microorganisms of 4%.

E-26 On day 402 the microorganisms obtained are subjected to an increase of stabilized organic waste and maintaining forced aeration, incubating at 70 ° C, registering a mortality of 6%.

RESULTS OBTAINED Table No .2. Comparative Table Initial and Final Characteristics.

The microorganism presents a normal activity in this medium being in its cellular membrane traces of Nitrogen.

It is also observed that the organic material constituted in 80% of carbohydrates stabilizes in a period of 35 dxas on average and unlike the wild microorganism with which the time was 120 days.

Clostridium pasteuranium Colonies are re-planted every 24 hours - Green ram coloration of bad uita Arthrobacter globiformis It is a Gram positive bacterium, non pathogenic, sporophobic, aerobic, its initial size is 1.2 μt wide x 2.5 μta long on average, the optimal growth temperature ranges between 20 and 30 ° C. Its activity develops in a pH of 6.5 to 7.0 and with a humidity of 90%.

The cells of Arthrobacter sp. in complex media, they undergo a marked change in their shape throughout the growth cycle. The older cultures consist of coccoid cells, in other stages they are bacillary. The cells are immobile or mobile by means of a polar flagellum or several lateral flagella. It does not form endospores. Chemoorganotrofo. They form little or do not form acid from glucose. Bacteria typically from the soil.

For its maintenance in the laboratory it was cultivated in a nutritious agar with the minimum essential elements, establishing pH, humidity and optimal temperature.

STAGES PERFORMED IN THE LABORATORY E-1 On day 1 the strain was sown in a modified medium, altering the humidity from 90% to 85%, after incubation there is a slow growth until 48 hours and a mortality of 14%.

E-2 On day 16 the previously obtained strains were reseeded in a modified medium, altering the temperature from 30 ° C to 35 ° C. A mortality of 10% is recorded.

E-3 On the 26th the microorganisms obtained were sown with the addition of -5% organic waste. This change allowed a slow development and a mortality of 14% of the microorganisms was presented.

E-4 On day 46 the microorganisms obtained are reseeded, with the addition of traces of organochlorines, showing a mortality of 48%.

E-5 On day 96, the microorganisms obtained undergo a second humidity change, from 85% to 80%. A mortality of 15% was recorded. E-6 On day 119 the strains are reseeded in the culture medium but altering the temperature from 35 ° C to 40 ° C. A mortality of 32% is recorded.

E-7 On day 184 the obtained strain was subjected to a change in the culture medium, adding 10% of dry and crushed organic material, maintaining the other parameters. A mortality of 10% is presented.

E-8 On day 194, organophosphate traces were added to the culture medium of the adapted microorganisms, showing a 43% mortality.

E-9 On day 214 the microorganisms obtained are reseeded, and the culture medium is altered from 80% to 75%, maintaining the other parameters, showing a mortality of 28%.

E-10 On day 264 the strains are reseeded by modifying the culture medium, altering the temperature from 40 ° C to 42 ° C. A mortality of 15% is recorded.

E-11 On day 287 the culture medium of the microorganisms is added 15% of dried and crushed organic material, the other parameters are maintained. There is a mortality of 52%.

On day 352 the adapted microorganisms were subjected to a change increasing in 5% mercurial traces, showing a mortality of microorganisms of 42%.

On day 382 the microorganisms are subjected to a change in humidity, from 75% to 70% the other parameters are maintained, showing a mortality of 27%.

On day 427 the strains were reseeded by modifying the culture medium, altering the temperature from 42 ° C to 45 ° C. A mortality of 24% is recorded.

On day 455, 20% of organic waste was added to the culture medium maintaining the other parameters, showing a mortality of 35%.

On day 491 the microorganisms underwent a change in the culture medium, increasing in 10% traces of organochlorines, maintaining the other parameters. A mortality of 37% is presented.

E-17 On day 521 the adapted microorganisms were subjected to a humidity change of 70% to 65%, maintaining the other parameters, presenting a mortality of 24%.

E-18 On day 569 the strains were reseeded modifying the culture medium, altering the temperature from 45 ° C to 50 ° C, registering a mortality of 22%.

E-19 On day 589, 25% of organic waste was added to the culture medium maintaining the other parameters, showing a mortality of 28%.

E-20 On day 622 the microorganisms were subjected to a change in the culture medium, increasing in 10% traces of organophosphates, maintaining the other parameters, presenting a mortality of 30%.

E-21 On day 645, the culture medium of the microorganisms was subjected to a humidity change, from 65% to 60%, keeping the other parameters incubating at 50 ° C. In this trial, a mortality of 37% of the population is presented.

E-22 On day 685 the strains were reseeded modifying the culture medium, altering the temperature range from 50 ° C to 55 ° C, registering a mortality of 19%.

E-23 On day 706 the adapted microorganisms underwent a change in the culture medium, increasing to 30% the organic residues, maintaining the parameters, presenting a mortality of 24%.

E-24 On day 741 the microorganisms adapted to the culture medium increased mercurial traces by 10%, maintaining the parameters, showing a mortality of 26%.

E-25 On day 778 the humidity level was changed from 60% to 55% to the culture medium of the microorganisms obtained, keeping the other parameters, incubating at 55 ° C. There is a mortality of 17%.

E-26 On day 818 the strains were reseeded by modifying the culture medium, altering the temperature from 55 ° C to 60 ° C, registering a mortality of 17%.

E-27 On day 845 the obtained strain was subjected to a change in the culture medium, adding 35% of dry and crushed organic material, maintaining the other parameters, showing a mortality of 26%.

E-28 On day 878 traces of organochlorines were added in 15% to the culture medium of the adapted microorganisms, the other parameters are maintained, showing a mortality of 42%.

E-29 On day 913 the humidity level of 55% to 50% was altered to the culture medium of the adapted microorganisms, maintaining the other parameters and incubating at 65 ° C. There was a mortality of 45%.

E-30 On day 946 the strains were reseeded modifying the culture medium, altering the temperature range from 60 ° C to 65 ° C, registering a mortality of 16%.

E-31 On day 971 the organic material dried and crushed to the culture medium of the obtained strain was increased to 50%, maintaining the other parameters, showing a mortality of 24%.

E-32 On day 1001 traces of organophosphates were increased by 15% to the culture medium of the adapted microorganisms, the other parameters are maintained, showing a mortality of 26%.

E-33 On day 1026 the humidity level of 50% to 45% of the culture medium of the obtained microorganisms is altered, maintaining the other parameters and incubating at 65 ° C. There is a mortality of 23%.

E-34 On day 1056 the strains were reseeded modifying the culture medium, altering the temperature from 65 ° C to 68 ° C, registering a mortality of 14%.

E-35 On day 1077, the dry and crushed organic material of the culture medium of the obtained strain was increased to 55%, maintaining the other parameters, showing a mortality of 25%.

E-36 On day 1104 traces of organophosphorus were increased by 20% of the culture medium, the other parameters were keep and incubate at 68 ° C. There is a mortality of 32%.

E-37 On day 1145 the moisture content of 45% to 40% of the culture medium of the obtained microorganisms is altered, maintaining the other parameters and incubating at 68 ° C. There is a mortality of 23%.

E-38 On day 1177 the strains were reseeded modifying the culture medium, altering the temperature of 68 ° C to 70 ° C, registering a mortality of 12%.

E-39 On 1192, the dry and crushed organic material of the culture medium of the obtained strain was increased to 60%, maintaining the other parameters, showing a mortality of 18%. E-40 On day 1213 traces of mercurials were increased by 25% of the culture medium, the other parameters were maintained and incubated at 68 ° C. A mortality of 12% is presented.

E-41 On day 1250 the humidity level of 40% to 35% of the culture medium of the microorganisms obtained is altered, maintaining the other parameters and incubating at 70 ° C. A mortality of 15% is presented.

E-42 On day 1285 the strains were reseeded by modifying the culture medium, altering the temperature from 70 ° C to 72 ° C, registering a mortality of 10%.

E-43 On day 1297 traces of organochlorines were increased by 30% of the medium-culture of the adapted microorganisms, the other parameters are maintained and incubated at 72 ° C. There is a mortality of 7%.

RESULTS OBTAINED Table No. 3 Comparative Table The microorganism presents a normal activity in said medium, with traces of Nitrogen in its cell membrane.

It is also observed that the organic material constituted in 80% of carbohydrates stabilizes in a period of 35 days on average and unlike the wild microorganism with which the time was 120 days.

ARTHROBACTER globiformis - GRAMPOSITIVO A GRAMNEGATIVA- AEROBI / Colony counts are performed every 12 hours - Gram green malachite colorations Bacillus megaterium It is a Gram positive bacillus, spore-forming, aerobic or facultative anaerobe; its initial size is 1.5 μ? wide x 5 μt? long on average, its optimum growth temperature ranges from 3 to 45 ° C and its activity develops within a pH of 3 to 5.5, with a humidity of 60%. The spores that it produces allow it to resist hostile environments, either by heat or drought.

The culture medium used for B. megaterium is a nutrient medium, consisting of peptone, distilled water, yeast extract and NaCl.

STAGES PERFORMED IN THE LABORATORY E-1 On day 1 the pH was changed from 5.5 to 5.7 to the culture medium and incubated at 45 ° C, keeping the other parameters of the initial culture medium constant, registering a mortality of 12%.

E-2 On the 11th, the strains were replanted, modifying the culture medium, altering the humidity from 60% to 55%, registering a mortality of 18%.

E-3 On day 36 the strains were reseeded by modifying the culture medium, altering the temperature from 45 ° C to 48 ° C, registering a mortality of 22%.

E-4 On day 49 the microorganisms obtained were reseeded, maintaining the same parameters of the culture medium, adding traces of organochlorines, showing a 28% mortality of the microorganisms.

E-5 On day 74, 10% organic waste was added to the culture medium of the microorganisms obtained, showing a mortality of 25%.

E-6 On day 102 the microorganisms obtained are reseeded, maintaining the same conditions until this stage, a second change is made to the pH of 5.7 to 6.0. There was a mortality of 22%.

E-7 On day 119 the humidity is changed from 55% to 50% of the culture medium of the obtained microorganisms. A mortality of 24% was registered.

E-8 On day 139 the strains were reseeded modifying the culture medium, altering the temperature from 48 ° C to 50 ° C, registering a mortality of 20%.

E-9 On day 154 the adapted microorganisms were subjected to a change, adding traces of organophosphates to the culture medium, showing a 30% mortality of the microorganisms.

E-10 On day 189 the obtained strain was subjected to a change in the culture medium, adding to 15% dry and crushed organic material, the other parameters are maintained, showing a mortality of 23%.

E-ll On day 209, the culture medium of the obtained microorganisms was subjected to a third pH change from 6.0 to 6.5, with a mortality of 15%.

E-12 On day 223 the microorganisms obtained are reseeded, and the culture medium is altered by 50% humidity. 48% maintaining the other parameters, presenting a mortality of 12%.

E-13 On day 247 the strains were reseeded by modifying the culture medium, altering the temperature from 50 ° C to 52 ° C, registering a mortality of 18%.

E-14 On day 263 the adapted microorganisms undergo a change adding mercurial traces, presenting a mortality of the microorganisms of 26%.

E-15 On day 295, 20% of organic material was added dried and crushed to the medium. culture of the microorganisms, maintaining the other parameters, showing a mortality of 28%.

E-16 On day 325 the adapted microorganisms undergo a pH change of 6.5 to 6.8, maintaining the other parameters and presenting a mortality of 8%.

E-17 On day 337 these microorganisms were subjected to a change in humidity, from 48% to 45%, maintaining the other conditions, showing a mortality of microorganisms of 10%.

E-18 On day 355 the strains were reseeded by modifying the culture medium, altering the temperature of 52 ° C, registering a mortality of 15%.

E-19 On day 367 the microorganisms underwent a change in the culture medium, increasing in 10% traces of organochlorines, maintaining the other parameters. In this stage, mortality was 20%.

E-20 On day 395, 25% organic waste was added to the culture medium, maintaining the other parameters, showing a mortality of 20%.

E-21 On day 420 the obtained strain was subjected to a pH change of 6.8 to 7.0, maintaining the other parameters, showing a mortality of 5%.

E-22 On day 430 the adapted microorganisms were subjected to a humidity change, from 45% to 40%, maintaining the other parameters, showing a mortality of 8%.

E-23 On day 445 the strains were reseeded modifying the culture medium, altering the temperature from 55 ° C to 60 ° C, registering a mortality of 10%.

E-24 On day 460 the microorganisms are subjected to a change in the culture medium, increasing in 10% traces of organophosphorus, maintaining the other parameters. In this stage a mortality of 15% is presented.

E-25 On day 475, 30% of organic waste was added to the culture medium, maintaining the other parameters, with a mortality of 16%.

E-26 On day 495, the microorganisms obtained undergo a change in pH, from 7.0 to 7.2, maintaining the other parameters of the culture medium, with a mortality of 5%.

E-27 The 505 day was subjected to a humidity change of 40% to 38% to the culture medium, maintaining the other parameters and incubating at 60 ° C, presenting a mortality of 7%.

E-28 On day 517 the strains are reseeded modifying the culture medium, altering the temperature from 60 ° C to 65 ° C; With this change, a mortality rate of 12% was registered.

E-29 On day 523 with the adapted microorganisms, mercurial traces were increased to 10% in the culture medium, the other parameters were maintained, and a microorganism mortality of 17% was presented.

E-30 On day 531 the adapted microorganisms underwent a change in the culture medium, increasing in 40% the organic residues, maintaining the other parameters, registering a mortality of 8%.

E-31 On day 536 the humidity is changed 38% to 36% to the culture medium of the microorganisms obtained, maintaining the other parameters and incubating at 65 ° C. There was a mortality of 12%.

E-32 On day 541 the strains are reseeded modifying the temperature from 65 ° C to 70 ° C, the other parameters of the culture medium are maintained, this change registers a mortality of 15%.

E-33 On day 544 to the culture medium of the adapted microorganisms, traces of organochlorines are increased by 15%, the other parameters are maintained, showing a mortality of 10%.

E-34 Day 549 is subjected to the culture medium of the obtained strain to a change, adding a 50% concentration of dry and crushed organic material, maintaining the other parameters, showing a mortality of 8%.

E-35 On day 554 the humidity is reduced from 36 to 32% incubating at 70 ° C the culture medium of the adapted microorganisms, the other parameters are maintained, showing a mortality of the microorganisms of 15%.

E-36 On day 572 the strains are reseeded by modifying the temperature from 70 ° C to 72 ° C, the other parameters of the culture medium are maintained, this change registers a mortality of 12%.

E-37 On day 597 traces of mercurials increase by 10% to the culture medium of the microorganisms adapted, the other parameters are maintained, presenting a mortality of 18%.

E-38 Day 644 is subjected to a change to the culture medium of the obtained strain, adding a 55% concentration of dry and crushed organic material, maintaining the other parameters. In this trial a mortality of 10% is presented.

E-39 At day 679 the humidity decreases from 32 to 30% incubating at 72 ° C of the culture medium of the adapted microorganisms, the other parameters are maintained, showing a mortality of the microorganisms of 8%.

E-40 On day 699 the strains are reseeded modifying the temperature from 72 ° G to 75 ° C, the other parameters of the culture medium are maintained, this change registers a mortality of 5%.

E-41 On day 724 organ traces of phosphorus are increased by 10% to the culture medium of the microorganisms adapted, the other parameters are maintained, presenting a mortality of 5%.

E-42 Day 759 is subjected to a change adding a 60% concentration of dry and stabilized organic material to the culture medium of the obtained strain, maintaining the other parameters, showing a mortality of 8%.

E-43 On day 799 the humidity is reduced from 30% to 28% by incubating the culture medium of the adapted microorganisms at 75 ° C, the other parameters are maintained, showing a mortality of the microorganisms of 4%.

RESULTS OBTAINED At the end of the adaptation process, the medium and the cultivation characteristics were the following: Table No.4 Comparative Table The microorganism presents a normal activity in these conditions, being in its membranes traces of Nitrogen.

It is also observed that the organic material constituted in 80% of carbohydrates stabilizes in a period of 35 days on average, unlike the wild microorganism with which the time was 120 days.

BASILLUS MEGATERIUM - GRAMPOSITIVE | AEROBICS or OPERATIVE ANAEROBICS Colony counts are performed every 48 hours - Gram green colorations of malac Bacillus subtillis It is a Gram positive bacillus, esporógeno, strict aerobic, with a thick layer of murein, its initial size is on average of 0.75 μt? of thickness x 0.9 μt? long; Its ambient temperature of growth oscillates between 15 to 55 ° C, given that the natural habitat of B. subtilis is the soil, which is subject to great fluctuations in temperature. However, the cells of this microorganism undergo a phenotypic change when the temperature is changed from 30 ° C to 45 ° C or to 80 ° C. Its activity develops within a pH of 4.7 to 5.5, a humidity of 70 to 80% and accepts levels of minimum toxic concentration, represented in traces.

It is used for the production of an antibiotic called bacitracin, which acts against Gram-negative by damaging its cell membrane and inhibiting wall formation. Also in the production of enzymes such as bacterial amylase useful in the paper and textile industry, and enzyme applied to tan leather, remove stains and soften meat.

The Microcosm Model was used for the isolation of microorganisms (Kabir et al., 1995). The strains were classified by traditional methods (Taxonomy numerical) and immunochemical (indirect immunofluorescence-IFI, according to Llewot and Stead, 1991). Pure strains from different sectors were used. The culture medium used for B. subtilis is a nutritive medium, with 5% agar-soybeans maintaining the pH parameters, minimum oxygen levels, humidity and temperature of its original state.

STAGES PERFORMED IN THE LABORATORY E-1 On day 1 the culture medium is changed to the initial pH of 5.9, keeping the other parameters constant. With this change, growth is slow and mortality is 68%.

E-2 On day 46 the strains were reseeded modifying the culture medium and 10% organic matter was added; With this change, a mortality of 52% was recorded.

E-3 On day 86 with the microorganisms obtained, maintaining the same parameters of the culture broth, they undergo a temperature change of 40 ° C to 55 ° C, presenting a mortality of 14%.

E-4 On day 106 a reseeding of microorganisms is carried out, maintaining the same parameters of the culture broth, except for humidity, which is altered from 80% to 70%, presenting a low mortality of 7%.

E-5 On day 126 a reseeding of microorganisms is carried out, maintaining the same parameters, increasing traces of organochlorines, showing a mortality in the culture of microorganisms by 78%.

E-6 On day 186 a reseeding of microorganisms is performed, a second pH change of 5.9 to 6.1 is made to the culture medium. There was a mortality of 31%.

E-7 On day 251 the obtained strain was subjected to a change in the culture medium, adding 15% of dry and crushed organic material, keeping the other parameters constant. In this stage there was a mortality of 8% of the population.

E-8 On day 271 the adapted microorganisms are subjected to a temperature change of 57 to 59 ° C, keeping the other parameters; this change presents a mortality of 24%.

E-9 On day 296 the microorganisms obtained undergo a change in humidity, from 70% to 65%, presenting a mortality of 55%, mobility and growth is greater, visualizing a change at the phenotype level.

E-10 On day 346 with the microorganisms obtained a reseeding is performed, maintaining the same parameters, adding traces of organophosphates, showing a mortality of 65%.

E-ll On day 394 the microorganisms undergo a third pH change from 6.1 to 6.5, keeping the other parameters; In this change, mortality was 45%.

E-12 On day 514 the microorganisms are subjected to a change in the culture medium, adding 20% of dry and crushed organic material, maintaining the other parameters and incubating at 59 ° C. In this trial a mortality of 22% is presented.

E-13 On day 524 the adapted microorganisms are subjected to a temperature change of 59 to 62 ° C, with a mortality of 17%.

E-14 On day 531 these microorganisms are subjected to a change in humidity, from 65% to 55%, maintaining the other parameters, there was a mortality of microorganisms of 58%.

E-15 On day 596 with the microorganisms obtained a reseeding is performed, maintaining the same parameters, adding mercurial traces, showing a mortality of 68%.

E-16 On day 626 the microorganisms obtained undergo a fourth pH change, from 6.5 to 6.8, maintaining the other parameters of the culture medium, showing a mortality of 24%.

E-17 On day 641 was added to the culture medium of the obtained strain, 25% of organic material dried and crushed, keeping the other parameters of humidity, temperature and incubating at 62 ° C. In this stage there was a mortality of 24% of the population.

E-18 On day 656 the adapted microorganisms are subjected to a temperature change of 62 ° C to 65 ° C, maintaining the parameters of humidity, culture medium, concentrations of chemical traces and pH, presenting a mortality of 20%.

E-19 On day 681 these microorganisms undergo a change in humidity, from 55% to 53%, maintaining the other parameters, showing a mortality of microorganisms of 58%.

E-20 On day 746 with the microorganisms obtained a reseeding is performed, maintaining the same parameters, adding traces of organochlorines in 10%, presenting a mortality of 36%.

E-21 On day 801 the microorganisms obtained undergo a fifth pH change of 6.8 to 7.0, maintaining the other parameters of the culture medium; presenting a mortality of 2%.

E-22 On day 816, the culture medium of the microorganisms was added with 30% of dried and crushed organic material, maintaining the other parameters of humidity, temperature and incubating at 65 ° C. In this stage there was a mortality of 5% of the population.

E-23 On day 831 the adapted microorganisms are subjected to a temperature change of 65 ° C to 68 ° C, maintaining the parameters of humidity, culture medium and pH, presenting a mortality of 7%.

E-24 On day 841 the adapted microorganisms undergo a humidity change, from 53% to 50%, maintaining the other parameters, registering a mortality of 23%.

E-25 On day 856 with the adapted microorganisms, traces of organophosphates increase by 10% in the culture medium, maintaining the other parameters, registering a mortality of 68%.

E-26 On day 886 the microorganisms obtained undergo a sixth pH change, from 7.0 to 7.2, keeping the rest parameters of the culture medium and incubating at 68 ° C. There was a mortality of 17%.

E-27 On day 896 the obtained strain is subjected to a change in the culture medium, adding 40% of dry and crushed organic material, maintaining the other parameters of humidity, temperature and incubating at 68 ° C. A mortality of 13% is recorded.

E-28 On day 904 the adapted microorganisms are subjected to a temperature change of 68 to 72 ° C, maintaining the other parameters of the culture medium, showing a mortality of 10%.

E-29 On day 911 the adapted microorganisms are subjected to a humidity change of 50% to 45%, maintaining the other parameters of the culture medium, presenting a mortality of 12%.

E-30 On day 926, the mercury traces were increased by 10% to the culture medium of the microorganisms obtained, maintaining the other parameters, showing a mortality rate of 32%.

E-31 On day 954 to the culture medium of microorganisms was added 50% of dry and crushed organic material, maintaining the other parameters of humidity, temperature and incubated at 12 ° d, presenting a mortality of 14% of the population.

E-32 On day 975, the microorganisms obtained were subjected to a humidity change, from 45% to 40%, with a mortality rate of 16%.

E-33 On day 1023 a medium temperature change of 72 ° C to 75 ° C was made to the culture medium of the microorganisms obtained, with a mortality of 20%.

E-34 On day 1054 the culture medium of the microorganisms obtained is made an increase of organic material to the culture medium, incubating at 75 ° C, presenting a mortality of 18%.

E-35 On day 1118 a change in humidity is made to the culture medium of the microorganisms obtained, decreasing it from 40 to 36% and incubating at 75 ° C, presenting a mortality of 20%.

E-36 On day 1178 the culture medium of the microorganisms obtained was changed by increasing the incubation from 75 to 78 ° C, with a mortality of 10%.

E-37 From day 1198 the strains are maintained in an incubation at 78 ° C, humidity of 36% and a culture medium with more than 50% of stabilized organic material, presenting a mortality of 14%.

RESULTS OBTAINED The characteristics of the medium are as follows (Table No. 5): Table No. 5. Comparative Table Initial and Final Characteristics At the end of the adaptation process, the microorganism presents a normal activity in this medium, and phosphorus traces (P2O5) are found in its cell membrane.

It is also observed that the organic material constituted in 80% of carbohydrates stabilizes in a period of 35 days on average unlike the wild microorganism with which the time was 120 days.

BASILLUS SUBTILLIS - GRAMPOSITIVO - AEROBIO STRICTO Colony counts are performed every 24 hours using green ram colorations of bad uita Bacillus stearother ophilus This species is a Gram positive, thermophilic, spore-forming, aerobic or anaerobic facultative bacillus. Its initial size is 0.9 μ? T? wide x 3.5 μp \ long on average, its optimum growth temperature ranges from 30 to 75 ° C, its cell membrane is completely smooth, and its activity develops in a pH range of 2.0 to 4.5 and a humidity of 60 %. It is one of the important bacteria when the temperature increase begins. This microorganism is usually dominant in areas with temperatures around 55 ° C.

The culture medium is nutritious, with 5% agar-soybeans maintaining the pH parameters, minimum levels of oxygen, humidity and temperature that its original state required.

STAGES PERFORMED IN THE LABORATORY E-1 On day 1 the culture medium is changed the initial acidity degree, taking it to a pH of 4.7 keeping the other parameters constant, registering a mortality of 16%.

E-2 On day 21 the strains are reseeded modifying the culture medium, altering the humidity from 60% to 55%; With this change, a mortality of 24% is registered.

E-3 On day 66 the microorganisms obtained, maintaining the same parameters of the culture broth, undergo a change, increasing the percentage of organic waste by 10%; this change presents a mortality of 32% of the microorganisms.

E-4 On day 116 the microorganisms obtained are reseeded, maintaining the same parameters of the culture broth, adding traces of organochlorines, showing a mortality of microorganisms of 38%.

E-5 On day 176 the microorganisms obtained are reseeded, maintaining the same conditions until this stage; a second pH change is made from 4.7 to 5.0. There was a mortality of 32%. Those who resisted the change had a delay in their growth.

E-6 On day 226 the microorganisms obtained, a second change to humidity is made, from 55% to 50%. A mortality of 25% was recorded. Those who resisted the change had a delay in their growth.

E-7 On day 271, the strain obtained was subjected to a change in the culture medium, adding dry and crushed organic material up to 15%, maintaining the other parameters. In this stage there is a mortality of 42% of the population.

E-8 The day 301 was subjected to microorganisms adapted to a change, adding traces of organophosphates, showing a mortality of microorganisms of 27%.

E-9 On day 346 the microorganisms obtained underwent a third pH change from 5.0 to 5.5; presenting a mortality of 15% and there is a growth in its structure.

E-10 On day 366 the microorganisms obtained are reseeded, and the culture medium is altered by 50% humidity. 45% maintaining the other parameters, presenting a mortality of 25%.

E-ll On day 411, 20% of dried and crushed organic material was added to the culture medium of the microorganisms, keeping the other parameters. In this stage there was a mortality of 40% of the population.

E-12 On day 486 the microorganisms were subjected to a change in the culture medium, adding mercury chemical traces, maintaining the other parameters. In this stage, mortality was 33%.

E-13 On day 546 the adapted microorganisms undergo a pH change from 5.0 to 6.0, maintaining the other parameters to which the strain has been subjected, presenting a mortality of 15%.

E-14 On day 564 these microorganisms are subjected to a humidity change, from 45% to 42%, maintaining the other parameters; presenting a mortality of 12%.

On day 574, 25% organic waste was added to the culture medium, maintaining the other parameters, registering a mortality of 33%. 16 On day 619 the microorganisms obtained are reseeded in a culture medium, increasing traces of organocerated by 10%, maintaining the other parameters, registering a mortality of 22%.

E-17 On day 644 the obtained strain was subjected to a pH change of 6.0 to 6.5, keeping the other parameters. In this stage, a mortality of 20% of the population was presented.

E-18 On day 659 the adapted microorganisms are subjected to a humidity change of 42% to 40%, maintaining the other parameters, presenting a mortality of 16%.

E-19 On day 677, 30% of organic waste was added to the culture medium, maintaining the other parameters, with a mortality of 15%.

E-20 On day 697, traces of organophosphates were increased by 10% in the culture medium of the adapted microorganisms, with a mortality rate of 12%.

E-21 On day 715 the microorganisms obtained undergo a pH change of 6.5 to 6.8, maintaining the other parameters of the culture medium, showing a mortality of 8%.

E-22 On day 722 the culture medium of the microorganisms was subjected to a humidity change of 40% to 38%, maintaining the other parameters and incubating at 75 ° C. In this trial, mortality of 18% of the population is presented.

E-23 On day 747 the adapted microorganisms undergo a change in the culture medium, increasing organic waste by 40%, maintaining the other parameters, with a 20% mortality rate.

E-24 On day 762 with the microorganisms adapted to the culture medium, the mercurial traces were increased by 10%, maintaining the other parameters, with a microorganism mortality of 15%.

E-25 On day 782, the culture medium was subjected to a pH change of 6.8 to 7.0, maintaining the other parameters, with a microorganism mortality of 15%.

E-26 On day 802 the culture medium was subjected to a humidity change, from 40% to 38%, maintaining the other parameters of the culture medium and incubating at 75 ° C. There was a mortality of 10%. · E-27 On day 817 the obtained strain was subjected to a change in the culture medium, adding 50% of dry and crushed organic material, keeping the other parameters. In this trial, mortality was 8%.

E-28 On day 824 with the adapted microorganisms, traces of organochlorines were increased in the culture medium. 15%, maintaining the other parameters, showing a mortality of microorganisms of 18%.

E-29 On day 849 the adapted microorganisms were subjected to a change in pH, from 7.0 to 7.2, maintaining the other parameters of the culture medium, presenting a mortality of 15%.

E-30 On day 861 humidity is changed from 38% to 36% to the culture medium of the microorganisms obtained, keeping the other parameters, incubating at 75 ° C. There was a mortality of 12%.

E-31 On day 876 the organic matter is increased to the culture medium of the microorganisms by 55%, maintaining the other parameters, showing a mortality of the microorganisms of 6%.

E-32 On day 876 traces of organophosphorus are increased by 15% to the culture medium of the microorganisms, maintaining the other parameters, showing a mortality of microorganisms of 4%.

RESULTS OBTAINED At the end of the adaptation process, the microorganism presents a normal activity in the medium, being found in its cell membrane traces of phosphorus (P2O5).

The characteristics of the culture medium and microbial development were as follows: Table No. 6. Comparative Table Initial and Final Characteristics It is also observed that the organic material constituted in 80% of cellulolytic fibers stabilizes in a period of 35 days on average, unlike the wild microorganism with which the time was 120 days.

BASILLUS Stearothermophilus - GRAMPOSITIVE - AEROBICS or OPERATIVE ANAEROBICS Colony counts are performed every 48 hours - Gram green colorations of mala uita Cellulomonas flavigena Gram positive gram-positive bacilli, irregular, 0.5 μt? in diameter by 0.7-2.0 fíra or more in length, which sometimes are straight, angular or curved. As the crop ages, the bacilli shorten. Its optimum development occurs between 30 to 37 ° C of temperature. They are mobile, they move using a flagellum that is usually polar or subpolar. They are chemoorganotrophic. This bacillus is aerobic but can develop in conditions of anaerobiosis. It has cellulolytic activity.

The culture medium used for its maintenance is a nutritious medium, with 5% agar-soybeans maintaining the parameters of pH, humidity and optimum temperature for its growth.

STAGES PERFORMED IN THE LABORATORY E-1 On day 1, the microorganisms obtained were developed under the same parameters of the initial culture medium, but with the addition of 5% of organic residues, presenting a decrease or mortality of 12%.

E-2 On day 16 the strains are reseeded modifying the culture medium, altering the humidity from 90% to 85%, with this change a mortality of 10% is registered.

E-3 On day 26 the strains were replanted by modifying the culture medium, altering the temperature from 37 ° C to 40 ° C; With this change, a mortality rate of 14% is registered.

E-4 On day 46 the microorganisms obtained are reseeded, maintaining the same parameters of the culture broth, adding traces of organochlorines, showing a mortality of the microorganisms of 48%.

E-5 On day 96 the obtained strain is subjected to a change in the culture medium, adding 10% of dry and crushed organic material, keeping the other parameters constant. In this stage there is a mortality of 15% of the population.

E-6 On day 119, the culture medium of the obtained microorganisms is changed from 85% to 80% humidity. A mortality of 32% was recorded.

E-7 On day 184 the strains are reseeded modifying the culture medium, altering the temperature from 40 ° C to 45 ° C, with this change a mortality of 22% is registered.

E-8 On day 214 the culture medium of the adapted microorganisms is subjected to a change adding traces of organophosphates, showing a mortality of the microorganisms of 43%.

E-9 On day 259 to the culture medium of the microorganisms is added 15% of organic material dry and crushed, keeping the other parameters constant. In this stage there is a mortality of 24% of the population.

E-10 On day 286 the microorganisms obtained are reseeded, and the culture medium is altered the humidity range of 80% to 75% maintaining the other parameters, presenting a mortality of 35%.

E-ll On day 323 the strains are reseeded modifying the culture medium, altering the temperature range from 45 ° C to 50 ° C, with this change a mortality of 22% is recorded.

E-12 On day 353 the adapted microorganisms undergo a change adding mercurial traces, showing a mortality of microorganisms of 48%.

E-13 On day 401 a 20% organic waste is added to the culture medium maintaining the other parameters, with a mortality of 12%.

E-14 On day 421 these microorganisms are subjected to a humidity change, from 75% to 70%, the other conditions are maintained, showing a 30% mortality of the microorganisms.

E-15 On day 454 the strains are reseeded modifying the culture medium, altering the temperature from 50 ° C to 55 ° C; With this change, a mortality rate of 20% is registered.

E-16 On day 477 the microorganisms are subjected to a change in the culture medium, increasing the chemical traces of organophosphates by 10%, keeping the rest parameters. In this stage, a mortality of 37% is presented.

E-17 On day 517. 25% of organic waste is added to the culture medium, maintaining the other parameters, with a mortality of 15%.

E-18 On day 538 the adapted microorganisms are subjected to a humidity change of 70% to 65%, maintaining the other parameters, showing a mortality of 32%.

E-19 On day 573 the strains were replanted by modifying the culture medium, altering the temperature from 55 ° C to 60 ° C, with this change a mortality of 35% was recorded.

On day 610 with microorganisms adapted to the culture medium, traces of organophosphorus are added in 10%, maintaining the other parameters, showing a mortality of microorganisms of 42%.

E-21 On day 650 the adapted microorganisms undergo a change in the culture medium, increasing to 30% the organic waste, maintaining the other parameters, showing a mortality of 17%.

E-22 On day 677 the culture medium of the microorganisms is subjected to a humidity change, from 65% to 60%; maintaining the other parameters and incubating at 60 ° C. In this trial, a mortality of 26% of the population is presented.

E-23 On day 710 the strains are reseeded modifying the culture medium, altering the temperature range from 60 ° C to 65 ° C. With this change, a 38% mortality rate is recorded.

E-24 On day 795 with the adapted microorganisms, mercurial traces were added in a 10% to the culture medium, the other parameters that up to this stage had been submitted were maintained, showing a mortality of the microorganisms of 68%.

E-25 On day 882 the strain obtained is subjected to a change in the culture medium, adding 35% of organic material dry and crushed, keeping the other parameters. In this trial a mortality of 65% is presented.

E-26 On day 972 the humidity level is altered from 60% to 58%, to the culture medium, maintaining the other parameters and .incubating at 65 ° C. A mortality of 24% is presented.

E-27 On day 1057 the strains are reseeded modifying the culture medium, altering the temperature from 65 ° C to 68 ° C; With this change, a mortality of 43% is recorded.

E-28 On day 1109 with the adapted microorganisms, traces of organophosphates were added to the culture medium in 15%; the other parameters are maintained, showing a mortality of microorganisms of 40%.

E-29 On day 1159 the obtained strain is subjected to a change in the culture medium, adding 40% of dry and crushed organic material, maintaining the other parameters. In this trial, a mortality of 32% is presented.

E-30 On day 1214, with the microorganisms obtained, moisture was altered from 58% to 55% in the culture medium, maintaining the other parameters of the culture medium, incubating at 68 ° C. There is a mortality of 36%.

E-31 On day 1261 the strains are reseeded modifying the culture medium, altering the temperature from 68 ° C to 70 ° C; With this change, a mortality rate of 32% is registered.

E-32 On day 1302 with the adapted microorganisms, traces of organochlorines are added to the culture medium in 15%; the other parameters are maintained, with a 30% mortality of the microorganisms.

E-33 On day 1334, the strain obtained is subjected to a change in the culture medium, adding 50% of dry and crushed organic material, maintaining the other parameters. In this trial a mortality of 26% is presented.

E-34 On day 1359 with the microorganisms obtained, the humidity medium is changed from 55% to 50% to the culture medium, maintaining the other parameters of the culture medium, incubating at 70 ° C. There is a mortality of 23%.

E-35 On day 1380 the strains are reseeded modifying the culture medium, altering the temperature from 70 ° C to 72 ° C; With this change, a mortality rate of 28% is registered.

E-36 On day 1417 with the adapted microorganisms, mercurial traces increase by 15% in the culture medium; the other parameters that up to this stage have been maintained are maintained, with a mortality of the microorganisms of 26%.

E-37 The 1452 day is subjected to the strain obtained to a change in the culture medium, adding 55% of organic material dry and crushed, keeping the other parameters. In this trial, mortality of 18% is presented.

E-38 On day 1474 with the microorganisms obtained, the culture medium is altered from 50% to 45%, maintaining the other parameters of the culture medium, incubating at 72 ° C. A mortality of 15% is presented.

E-39 On day 1493 the strains are reseeded modifying the culture medium, altering the temperature from 72 ° C to 74 ° C; With this change, a mortality of 12% is registered.

E-40 On day 1510 with the adapted microorganisms, traces of organophosphates increase by 20% to the culture medium; the other parameters that up to this stage have been submitted are maintained, with a 17% mortality of the microorganisms.

E-41 On day 1530 the obtained strain is subjected to a change in the culture medium, adding dry and crushed organic material, maintaining the other parameters. In this trial, a mortality of 12% is presented.

E-42 On day 1544 with the microorganisms obtained, the culture medium is altered from 45% to 40%, maintaining the other parameters of the culture medium, incubating at 7 ° C. There is a mortality of 8%.

E-43 On day 1557 the strains are reseeded modifying the culture medium, altering the temperature of 74 ° C to 77 ° C; With this change, a mortality of 5% is registered.

E-44 On 1572 with the adapted microorganisms, traces of organochlorines are increased in the culture medium in a 25% the other parameters are maintained, with a mortality of the microorganisms of 12%.

E-45 On 1587 the obtained strain is subjected to a change in the culture medium, adding dry and crushed organic material, maintaining the other parameters. In this trial a mortality of 6% is presented.

E-46 On day 1593 the humidity is altered to the culture medium from 401 to 361, maintaining the other parameters of the culture medium, incubating at 11 ° Z. A mortality of 10% is presented.

E-47 On day 1613 traces of mercury were increased to the culture medium by 30% and incubated at 77 ° C, the other parameters were maintained, showing a mortality of 12%.

E-48 On day 1633 the obtained strain is subjected to a change in the culture medium, adding dry and crushed organic material, keeping the others, parameters. In this trial a mortality of 6% is presented.

E-49 On day 1650 the humidity is changed from 36% to 32%, maintaining the other parameters of the culture medium, incubating at 77 ° C. A mortality of 4% is presented.

RESULTS OBTAINED At the end of the adaptation process, the culture medium and the development characteristics of the microorganism were as follows (Table No 7): Table No. 7. Comparative Table Initial and Final Characteristics The growth and development of the microorganism is optimal with these physicochemical and environmental characteristics. In its cellular membrane presents traces of potassium.

It is also observed that the organic material constituted in 80% of cellulolytic fibers is stabilized in a period of 35 days on average, differing from the behavior of the wild strain of Cellulomonas. sp. whose biodegradation time is 120 days.

Cellulomonas flavigena - GRAMPOSITIVO A GRAMNEGATIVA- AEROBIAS Colony counts are done every 24 hours - Gram green coloration of mala uita Streptomyces thermonitrificans Are Gram-positive microorganisms, forming endospores, have irregular bacilli from 0.5 to 1.0 μt in diameter by 0.7 - 2.0 μp? or longer, which are sometimes straight, angular or curved. As the crop ages, the bacilli shorten. Its optimal development occurs in a range of 25 to 65 ° C of temperature. They are mobile, they move using a flagellum that is usually polar or subpolar. They are chemoorganotrophic. This bacillus is aerobic but can develop in conditions of anaerobiosis.

STAGES PERFORMED IN THE LABORATORY On day 1 the strains are replanted by modifying the culture medium, injecting forced aeration and decreasing the humidity from 90% to 85%, with this change a mortality of 8% is recorded and the total coverage of the field is gives at 2 days.

E-2 On the 8th the microorganisms obtained are reseeded, maintaining the forced aeration and the same parameters of the culture broth, adding traces of organoclorados, presenting a mortality of 43% and the field is filled at 8 days.

E-3 On day 20 the same parameters of the culture medium are maintained, and in addition 10% of pulverized and sterilized organic waste is added, this change presents a mortality of 40% of the microorganisms and the field is totally covered at 55 days.

E-4 On day 80 the obtained microorganisms are subjected to a humidity change of 85% to 80%. A mortality of 35% was registered, the total coverage of the field was given at 42 days.

E-5 On day 127 to the culture medium of the adapted microorganisms, traces of organophosphates are added, showing a mortality of the microorganisms of 45% and the coverage of the field is given at 58 days.

E-6 On day 190 a change in the culture medium is applied to the obtained strain, adding 15% of dry and crushed organic material, maintaining the other constant parameters. In this stage, a mortality of 37% of the population, totally covering the field at 41 days.

On day 236 the culture medium is altered the humidity range of 80% to 75% maintaining the other parameters, showing a mortality of 35% and the field is completely covered at 39 days.

E-8 On day 280 the adapted microorganisms are subjected to a change, adding traces of mercury, showing a mortality of microorganisms of 32%, the total coverage of the field is given at 55 days.

On day 340, 20% of dry and crushed organic material is added to the culture medium of the microorganisms, the other parameters are kept constant. In this stage there is a mortality of 34% of the population, recovering the field completely at 45 days.

E-10 On day 390 they undergo a change of humidity to these microorganisms, from 75% to 70%, keeping the rest conditions, presenting a mortality of the microorganisms of 30%; the total coverage of the field is recorded at 43 days.

E-ll On day 438 the microorganisms are subjected to a change in the culture medium, increasing in 10% the chemical traces of organophosphorus, maintaining the other parameters. In this stage, a mortality of 28% is presented.

E-12 On day 483, 25% of organic waste is added to the culture medium, maintaining the other parameters, with a mortality of 30%.

E-13 On day 535 they undergo a change of humidity to the adapted microorganisms, from 70% to 65%, maintaining the other parameters, presenting a mortality of 35%.

E-14 On day 590 microorganisms adapted to the culture medium are increased by traces of organophosphates by 10% and temperature by 65 to 72 ° C, the rest parameters that up to this stage have been submitted are maintained, showing a mortality rate of 28%.

E-15 On day 638, 30% organic waste is added to the culture medium, maintaining the other parameters, with a mortality of 25%.

E-16 The 691 day is subjected to a change of humidity range to the culture medium of the microorganisms, from 65% to 60%; maintaining the other parameters, incubating at 72 ° C. In this trial, a mortality of 22% of the population is presented.

E-17 On day 746 mercurial traces are added in 10% to the microorganisms adapted to the culture medium, the other parameters are maintained; presenting a mortality of microorganisms of 20%.

E-18 Day 786 is subjected to a change in the culture medium adapted microorganisms, increasing organic waste by 40%, maintaining the other parameters, showing a mortality of 21%.

E-19 On day 834 the humidity of the culture medium is altered, from 60% to 55%, maintaining the other parameters of the culture medium, incubating at 72 ° C. A mortality of 26% is presented.

E-20 On day 892 traces of organophosphorus are added in 15% to the culture medium of the adapted microorganisms, maintaining the other parameters, showing a mortality of the microorganisms of 24%.

E-21 On day 952, the obtained strain is subjected to a change in the culture medium, adding 50% of dry and crushed organic material, keeping the other parameters. In this trial a mortality of 20% is presented.

E-22 On day 987 the humidity is altered to the culture medium, from 55% to 50%, maintaining the other parameters of the culture medium, incubating at 72 ° C. A mortality of 15% is presented.

E-23 On day 1027 traces of organochlorines are increased by 15% to the culture medium of the adapted microorganisms; the other parameters are maintained, with a mortality of 12%.

E-24 On day 1057 the culture medium is subjected to a change, adding 50% of dry and crushed organic material, keeping the other parameters, incubating at 11 ° 0 .. There is a mortality of 20%.

E-25 On day 1099 the humidity level is altered to the culture medium, from 50% to 45%, maintaining the other parameters of the culture medium, incubating at 72 ° C. A mortality of 22% is presented.

E-26 On day 1144 mercurial traces were increased by 20% to the culture medium of the adapted microorganisms; the other parameters are maintained, presenting a mortality of 34%.

E-27 The 1209 day undergoes a change in the culture medium, adding 55% of dry organic material and crushed, keeping the other parameters, incubating at 72 ° C. A mortality of 30% is presented.

E-28 On day 1260 the humidity level is altered to the culture medium, decreasing from a range of 45% to 40%, maintaining the other parameters of the culture medium, incubating at 72 ° C. There is a mortality of 23%.

E-29 On day 1297 to the culture medium of the adapted microorganisms, organochlorine traces are increased in a 25%; the other parameters are maintained, with a mortality of 15%.

E-30 On day 1335 the culture medium is subjected to a change, adding 60% of dry and crushed organic material, keeping the other parameters, incubating at 72 ° C. There is a mortality of 16%.

E-31 On day 1370 the degree of humidity is altered to the culture medium, decreasing from a range of 40% to 35%, maintaining the other parameters of the culture medium, incubating at 72 ° C. There is a mortality of 8%.

RESULTS OBTAINED Table No.8. Comparative Table Initial and Final Characteristics.

The microorganism presents a normal activity in said medium, with traces of Nitrogen in its cell membrane.

It is also observed that the organic material constituted in 80% of carbohydrates stabilizes in a period of 35 days on average and unlike the wild microorganism with which the time was 120 days.

STREPTOMYCES thermonitrífícans - GRAPHICS - FACULTALY AEROBICS Replanting colonies every 24 hours - Gram green malachite colorations Bacillus brevis It is a Gram positive, non-pathogenic, sporogenic, facultatively anaerobic bacillus; its initial size is 0.85 μt wide x 1.2 μp? long on average, its optimum growth temperature ranges from 21 to 37 ° C and in total absence of oxygen. Its activity develops within a pH of 2.0 to 4.5, at an average humidity of 80%. This microorganism is obtained from the rumen.

The cells of B. brevis produce gramicidin, an antibiotic used against Gram-positive bacteria.

The culture medium used for B. brevis is nutritious medium, with 5% agar-soybeans maintaining the pH parameters, C02 levels and cyclic increase of oxygenation and direct radiation, humidity and temperature for optimal development.

STAGES PERFORMED IN THE LABORATORY E-1 On day 1, forced aeration at 2.5% was injected into the culture medium, maintaining the other parameters and incubation at 36 ° C. With this change, a mortality of 75%, therefore there is a slow growth of the colony.

E-2 On day 91, 5% forced aeration is injected into the culture medium and the humidity is altered from 80% to 75%, maintaining the. other parameters. With this change, a mortality of 25% is registered.

E-3 On day 115, 7.5% forced aeration was injected into the culture medium and the temperature was altered from 36 ° C to 40 ° C; With this change, a mortality rate of 48% is registered.

E-4, On day 160, 10% forced aeration was injected into the culture medium and traces of organophosphates were added, with this change a mortality of 68% was registered.

E-5 On day 232, 12.5% forced aeration was injected into the culture medium and 10% of organic waste was added, keeping the other parameters, this change registers a mortality of 30%.

E-6 On day 264, 15% forced aeration was injected into the culture medium and the pH was altered from 5 to 5.3, maintaining the other parameters, with this change a mortality of 70% was recorded.

E-7 On day 344 forced aeration is injected at 17.5% to the culture medium, humidity is altered, from 75% to 70% maintaining the other parameters of the culture broth, showing a mortality of 28%.

E-8 On day 376, 20% forced aeration is injected into the culture medium and the temperature is altered from 40 ° C to 45 ° C maintaining the other parameters of the culture broth, with a 38% mortality rate.

E-9 On day 411, 22.5% forced aeration is injected into the culture medium and traces of organophosphates are added, keeping the other parameters, showing a mortality of the microorganisms of 40%.

E-10 On day 473, forced aeration at 25% was injected into the culture medium and 15% of organic material was added, maintaining the other parameters, presenting a mortality of microorganisms of 25%.

On day 496, 27% forced aeration is injected into the culture medium and the pH is altered from 5.3 to 5.5. A mortality of 72% was recorded.

On day 569, 30% forced aeration is injected into the culture medium and humidity is changed from 70% to 65%. A mortality of 23% was recorded.

On day 589, forced aeration at 32.5% is injected into the culture medium and subjected to a change in temperature, from 45 ° C to 48 ° C, the other parameters are maintained. In this stage there is a mortality of 25% of the population.

On day 616, 35% forced aeration is injected into the culture medium and mercurial traces are added, keeping the other parameters. In this stage there is a mortality of 32% of the population.

E-15 On day 661, forced aeration of 37.5% was injected into the culture medium and it was increased to 20% of organic waste, registering a mortality of 43%.

E-16 On day 713, forced aeration at 40% was injected into the culture medium and the pH was changed from 5.5 to 5.8, with a mortality of 62%.

E-17 On day 767, forced aeration at 42.5% was injected into the culture medium and the humidity was changed from 65% to 60%, with a mortality of 20%, mobility and growth being low.

E-18 On day 792, 45% forced aeration is injected into the culture medium and the temperature is altered from 48 ° C to 50 ° C, mortality is registered at 18%, mobility and growth are low.

E-19 On day 815, forced aeration of 47.5% was injected into the culture medium and traces of organophosphates were increased by 10%, showing a mortality of 36%.

E-20 On day 858, forced aeration at 50% was injected into the culture medium, adding 25% of organic waste, showing a mortality of 25%.

E-21 On day 880, forced aeration of 52.5% was injected into the culture medium, altering the pH from 5.8 to 6.0; the other parameters are maintained; in this change, a mortality of 53% is presented.

E-22 On day 925, 55% forced aeration is injected into the culture medium and the humidity is altered, from 60% to 55%, the other parameters are maintained; in this change, mortality of 18% is presented.

E-23 On day 945 forced aeration is injected 57.5% into the culture medium and the temperature is altered from 50 ° C to 55 ° C, maintaining the other parameters. In this stage, a mortality of 20% is presented.

E-24 On day 971, 60% forced aeration is injected into the culture medium and traces of organophosphates are increased by 10%, keeping the rest parameters. In this trial a mortality of 25% is presented.

E-25 On day 1006 forced aeration is injected to 62.5% to the culture medium and 30% of stabilized organic waste is increased, registering a mortality of 32%.

E-26 On day 1049, 65% forced aeration is injected into the culture medium and the pH is altered, from 6.0 to 6.2, registering a mortality of 43%.

E-27 On day 1087 forced aeration is injected at 67.5% to the culture medium and the humidity is altered, from 55% to 50%, maintaining the other parameters, registering a mortality of 15%.

E-28 On day 1111 forced aeration is injected 70% to the culture medium and the temperature is altered, from 55 ° C to 60 ° C, maintaining the other parameters, presenting a mortality of 16%.

E-29 On day 1129, forced aeration at 72.5% was injected into the culture medium, increasing the traces of organophosphorus by 10%, maintaining the other parameters of the culture, showing a mortality of microorganisms of 27%.

E-30 On day 1162, 75% forced aeration is injected into the culture medium and 40% of organic waste is added, maintaining the other parameters of the culture, showing a 25% mortality of the microorganisms.

E-31 On day 1191, forced aeration of 77.5% was injected into the culture medium, increasing the pH from 6.2 to 6.5, maintaining the other parameters of the culture medium; presenting a mortality of 25%.

E-32 On day 1215, 80% forced aeration is injected into the culture medium and the humidity is reduced, from 50% to 45%, maintaining the other parameters of the culture medium, registering a mortality of 18%.

E-33 On day 1233, 82.5% forced aeration is injected into the culture medium and the temperature is altered from 60 ° C to 65 ° C, keeping the other parameters. There is a mortality of 12% of the population.

E-34 On day 1247, 85% forced aeration was injected into the culture medium and the mercurial traces were increased by 10%, maintaining the other parameters and incubating at 65 ° C. There is a mortality of 28% of the population.

E-35 On day 1277, forced aeration of 87.5% was injected into the culture medium and organic waste was increased to 50%, maintaining the other parameters, with a 15% mortality rate.

E-36 On day 1300, 90% forced aeration is injected into the culture medium and the pH is changed from 6.5 to 6.8, maintaining the other parameters, showing a mortality of 21%.

E-37 On day 1317, forced aeration of 92.5% was injected into the culture medium and humidity was altered from 45% to 40%, the other parameters were maintained. A mortality of 12% is recorded.

E-38 On day 1332, 95% forced aeration is injected into the culture medium and the percentage of mercurial traces is increased by 15%, keeping the other parameters. A mortality of 25% is recorded.

E-39 On day 1360 forced aeration is injected to 97.5% to the culture medium and the pH is changed from 6.8 to 7.0, the other parameters are maintained, presenting a mortality of 15%. ? -40 On day 1375 forced aeration is injected 100% to the culture medium and the temperature is changed from 65 ° C to 70 ° C, the other parameters are maintained, showing a mortality of 8%.

E-41 On day 1392 the culture medium was maintained with forced aeration, the pH was changed from 7.0 to 7.2, maintaining the other parameters of the culture medium; registering a mortality of 12%.

E-42 The day 1415 was maintained with forced aeration to 100% the culture medium, humidity is altered from 40% to 36%, maintaining the other parameters of the culture medium; registering a mortality of 25%.

E-43 On day 1443 the culture medium was maintained with 100% forced aeration, increasing the temperature level from 70 to 72 ° C and maintaining the other parameters of the culture medium; registering a mortality of 17%.

E-44 On day 1468 the culture medium was maintained with 100% forced aeration, incubating at 72 ° C and an increase in the stabilized organic matter of the culture medium was carried out, maintaining the other parameters of the culture medium; registering a mortality of 11%.

E-45 On day 1483 the culture medium was maintained with forced aeration, incubating at 72 ° C and an increase was made to the culture medium adding chemical traces, maintaining the other parameters of the culture medium; registering a mortality of 15%.

E-46 The day 1512 was maintained with 100% forced aeration of the culture medium, increasing the temperature level of the from 72 to 75 ° C and maintaining the other parameters of the culture medium; registering a mortality of 10%.

E-47 On day 1530 the culture medium was maintained with 100% forced aeration, incubating at 75 ° C and an increase was made to the culture medium adding chemical traces, maintaining the other parameters of the culture medium; registering a mortality of 7%.

RESULTS OBTAINED At the end of the adaptation process, the microorganism presents a normal activity in a medium with the following characteristics (Table No.9): Table No. 9. Comparative Table Initial and Final Characteristics As an important characteristic, B. brevis changes its condition of anaerobic respiration due to development under aerobic conditions.

It is also observed that the organic material constituted in 80% of cellulolytic fibers stabilizes in a period of 35 days on average, unlike the wild microorganism with which the time was 120 days.

BACILLUS BREVIS - GRAPHICS - OPACIOUS ANAEROBICS Colony counts are carried out every 24 hours - Green ram coloration of bad uita Bacillus thuringiensis It is a Gram positive bacillus, not pathogenic, sporagen, facultative anaerobe, its initial size is on average 1.2 μt wide x 4.8 μtt? long on average, its optimum temperature environment of growth ranges from 10 to 45 ° C, its cell membrane is completely smooth and its activity develops within a pH of 6.5 to 7.2 with a humidity of 60%.

The B. thuringiensis cells form crystalline inclusions visible under the optical microscope during sporulation. These crystalline inclusions are composed of protoxins, which contain d-endotoxins, of insect-specific insecticidal activity. Some varieties of Bacillus thuringiensis can also produce another toxin, b-exotoxin. It is a toxin that occurs during vegetative growth, and is a nucleotide derivative of adenine that functions as an inhibitor of RNA polymerase. But the use of this toxin is prohibited in some countries, because it is toxic also for mammals.

The culture medium to maintain B. thuringiensis is a nutritious medium, with 5% agar-soybeans maintaining the H parameters, minimum oxygen levels, humidity and temperature of its original state.

STAGES PERFORMED IN THE LABORATORY E-l On day 1, forced aeration at 5% is injected into the culture medium, keeping the other parameters constant. The incubation temperature was 50 ° C. With this change, a mortality rate of 16% was recorded, with an increase in the growth rate of the colonies and CFU / ml. E-2 On day 21, 10% forced aeration is injected into the culture medium and humidity is reduced from 60% to 55%; With this change, a mortality of 24% is registered, during which time it was necessary to inoculate it at 50 ° C.

E-3 On day 56, 15% forced aeration is injected into the culture medium and 10% organic waste is added, keeping the other parameters, incubating at 50 ° C, this change registers a mortality of 32%.

E-4 On day 106, 20% forced aeration is injected into the culture medium, maintaining the other parameters, they add mercurial traces, showing a 38% mortality of the microorganisms.

E-5 On day 166, forced aeration at 25% is injected into the culture medium and the temperature range of 50 ° C is altered to 55 ° C, maintaining the other parameters, presenting a mortality of 32%.

E-6 On day 216, 30% forced aeration is injected into the culture medium and the humidity range is changed from 55% to 50%. A mortality of 25% was recorded.

E-7 On day 261, forced aeration at 35% was injected into the culture medium and subjected to a change in the culture medium, adding 15% of dry and crushed organic material, maintaining the other parameters constant. In this stage there is a mortality of 42% of the population.

E-8 On day 351, 40% forced aeration is injected into the culture medium and subjected to an increase in chemical traces, adding traces of organophosphates, registering a mortality of 27%, mobility and growth is low.

E-9 On day 396, 45% forced aeration is injected into the culture medium and the temperature range of 55 ° C of 65 ° C is altered, with a mortality rate of 45%; mobility and growth is variable.

E - 10 On day 416, 50% forced aeration is injected into the culture medium and the humidity range is altered from 50% to 45% with a mortality of 25%.

E-ll On day 461, 55% forced aeration is injected into the culture medium and the percentage of organic waste is altered, increasing it to 20%, the other parameters of humidity, temperature, etc. remain; in this change a mortality of 40% was registered.

E-12 On day 536, 60% forced aeration is injected into the culture medium and the chemical traces are increased in organocerated by 5%, maintaining the other parameters, and incubating at 65 ° C. In this trial a mortality of 33% is presented.

On day 596, 65% forced aeration is injected into the culture medium and the temperature range of 65 ° C to 68 ° C is altered, with a mortality of 15%.

On day 614 forced aeration is injected 70% to the culture medium and the humidity range is altered from 45% to 40%, the other parameters are maintained, showing a mortality of the microorganisms of 33%.

On day 659, 75% forced aeration is injected into the culture medium and the percentage of organic waste is increased to 25%, maintaining the other parameters of the culture, with a mortality rate of 18% for microorganisms.

On day 684, 80% forced aeration was injected into the culture medium and the percentage of mercurial traces was increased by 10%, keeping the other parameters, showing a mortality rate of 28%.

On day 699, 85% forced aeration is injected into the culture medium and subjected to an increase in the range of temperature of 68 ° C to 70 ° C, presenting a mortality of 15%.

E-18 On day 719, 90% forced aeration is injected into the culture medium and the humidity range is altered, decreasing the range from 40% to 36%, maintaining the other parameters, with a mortality of 15%.

E-19 On day 739, 95% forced aeration is injected into the culture medium and the organic residues are increased to 30% by incubating at 70 ° C and maintaining the other parameters, with a mortality of the microorganisms of 12%.

E-20 On day 754 forced aeration is injected 100% to the culture medium and the percentage of chemical traces is increased by 10% organophosphorus, the other parameters are maintained. A mortality of 16% is recorded.

E-21 On day 772, 100% forced aeration to the culture medium, a 36% humidity range, is maintained, is subjected to a temperature increase of 70 ° C to 72 ° C, with a mortality of 35%.

E-22 On day 827 the forced aeration is maintained 100% to the culture medium and incubated at 72 ° C, the humidity range is reduced from 36% to 34%, maintaining the other parameters of the culture medium, registering a 29% mortality.

E-23 On day 872, forced aeration at 100% was maintained in the culture medium, incubation at 72 ° C, increasing the percentage of organic residues to the culture medium, at this stage there is a mortality of 20% of the population, there is more mobility and growth.

E-24 On day 887, forced aeration at 100% was maintained in the culture medium, and the percentage of chemical traces was increased by 10% organochlorines, registering a mortality of 15%. The turbidity is uniform.

E-25 On day 912, 100% forced aeration is maintained in the culture medium, it is subjected to an increase in temperature from 72 ° C to 74 ° C, registering a mortality of 15%.

The turbidity is uniform.

E-26 On day 927 the forced aeration is maintained 100% to the culture medium and incubated at 74 ° C, the humidity range is reduced from 34% to 32%, maintaining the other parameters of the medium of? ? ^ ??, registering a mortality of 8%.

E-27 On day 947, forced aeration at 100% was maintained in the culture medium, incubation at 74 ° C, increasing the percentage of organic residues to the culture medium, at this stage there is a 15% mortality of the population, there is more mobility and growth.

E-28 On day 962, forced aeration at 100% was maintained in the culture medium and the percentage of chemical traces was increased by 20% of mercury, registering a mortality of 10%. The turbidity is uniform.

RESULTS OBTAINED At the end of the adaptation process, the culture medium and microbial development have the following characteristics (Table No. 10): Table No. 10. Comparative Table Initial and Final Characteristics.

As an important characteristic, B. thuringiensis changes its facultative anaerobic condition, due to its development under aerobic conditions. and also observes that the organic material constituted by 80% of cellulolytic fibers stabilizes in a period 35 days on average, unlike the Ivestre microorganism with which the time was 120 days.

BASILLUS thuringiensis - GRAMPOSITIVO - ANAEROBIOS OPTIONAL | Colony counts are performed every 12 hours - Gram green malachite colorations FINALIZATION OF WORK OF ADAPTATION PRODUCTION INOCULO BIODEGRADADOR At the end of the adaptation work of the microorganisms, it is observed that the culture medium used for all is composed of: Conventional nutritive broth Soybean Agar 10% Organic material 50% pH 7 - 7.2 Temperature between 65 and 78 ° C Humidity between 30 and 36% For the above and taking into account the final characteristics of the adapted microorganisms, for obtaining the sample, these are added to said culture medium in amounts such that they fall within the following ranges: After obtaining the above mixture with the microorganisms in the laboratory, it is transported in conventional liquid medium in suitable containers that allow the maintenance of its characteristics and properties. The concentration of microorganisms is 2.5 - 3X10e CFU / ml.

INOCULATION IN BIODEGRADATION PROCESSES The process of inoculation of the mixture in the field includes the following steps: • Perform manually or mechanically a separation of the non-biodegradable waste, in case of arriving at a process without its proper selection and crushing by means of the use of machinery the material to allow a greater contact area and consequently a better action of microorganisms. Also maintain or obtain material moisture between 20 and 30%.

Submit the organic solid waste to be treated to a pH stabilization process from 7.0 to 7.2, using conventional methods for this purpose.

Organize and distribute the waste in bundles of 2.5 meters (m) high, by 3 m wide, by 10 or more in length, depending on the latter measure of the quantities to be handled.

Carry out a first inoculation taking into account that the highest percentage within the ranges, is the microorganisms that degrade carbohydrates and in a lesser proportion the cellulolytics. These begin the process of biodegradation in the initial or adaptation stage by gradually elevating the temperature product of their metabolic reactions. This increase reaches approximately up to 63 ° C. Perform a second inoculation of the mixture at 15 days, changing the percentages so that they are inverted to the previous inoculation, being the concentration of cellulolytic microorganisms and lower carbohydrate decomposers.

It is necessary to keep track of the process and establish the critical control points that guarantee the quality of the process. For this initially it is necessary to control and avoid the proliferation of insects, samurai rodents or carrion birds, as well as analyze possible generation of bad odors and leachates.

The microbiological process from the first inoculation has an approximate duration of 35 days, after which the biodegraded material is transferred to a greenhouse or suitable enclosure to perform screening operations and obtain adequate granulometry.

Claims (5)

CLAIMS Having described the invention as above, the content of the following claims is claimed as property:

1. A compound of microorganisms for biodegrading organic fraction contained in solid waste, characterized in that it comprises a mixture of the following microorganisms: Bacillus licheniformis, Bacillus brevis, Bacillus megaterium, Bacillus stearothermophilus, Bacillus subtilis, Bacillus thuringiensis, Clostridium pasteuranium, Streptomyces thermonitrificans, Arthrobacter globiformis, Celluíomonas fl vigena.

2. The compound according to claim 1, characterized in that said organisms are found at the following percentages: 5 to 15% of Bacillus licheniformis, 5 to 20% of Bacillus brevis, 5 to 15% of Bacillus megaterium, 5 to 10% of Bacillus stearothermophilus, 5 to 10% of Bacillus subtilis, 5 to 10% of Bacillus thuringiensis, 5 to 15% of Clostridium pasteuranium, 5 to 15% of Streptomyces thermonitrificans, 5 to 20% of Artárobacter globiformis, 5 to 10% of Cellulomonas flavigena.

3. The compound according to claim 1, characterized in that the ingredients of the culture medium of the compound and the conditions are: 40% conventional nutritive broth, 10% soy agar, 10% cellulose agar and 40% crushed organic matter and sterilized The conditions are: pH between 7.0 and 7.2, humidity between 30 and 36% and a temperature between 65 and 78 ° C.

4. The compound according to claim 1, characterized by being applied to an organic fraction that meets the following characteristics: a. Organic waste with a pH of 7.0 to 7.2 b. Free biodegradable material in 80% other materials. c. Moisture of organic material between 20 and 40% d. Construction of 3 m. at 11 m wide x 4.5 m at 6.5 m high and the length depends on the quantities handled which can be from 20 to 85 meters long.

5. A process for the preparation of the compound of claim 1, characterized in that it comprises the following steps: a) A first inoculation that must be carried out with a greater quantity of carbohydrate mineralizing microorganisms, on average 65 to 80% and a lower amount of cellulose mineralizers. b) A reinforcement with this same mixture after eight days. c) A first application at 15 days with the compound made up of 65% to 80% of cellulolytic fiber mineralizing microorganisms and smaller amount of carbohydrate mineralizing microorganisms. d) A fourth inoculation or second reinforcement with this microbial compound eight days later. e) A fifth inoculation with a third mixture of pathogen-controlling microorganisms.