KR20010053470A - Plant growth enhancement against phytopathogenic fungi and/or soil borne pests - Google Patents

Abstract

The present invention relates to a mixture of useful substances having plant-enhancing and / or plant-healing effects against attack by phytopathogenic fungi and / or soil pests, which comprises a mixture of alkyl (poly) glycosides of the o / w type An aqueous fatty alcohol formulation which is in combination with a compatible surfactant compound and / or the use of a lower polyhydric alcohol and a fatty acid partial ester.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a plant growth enhancer for plant pathogenic fungi and / or pests of soil origin,

Previously unpublished German Patent Application No. 197 01 127.6 describes low-foaming surfactant concentrates for use in promoting plant growth. More particularly, the patent application discloses a low-foaming wetting adjuvant in a highly concentrated form, or a flowable and spillable aqueous surfactant-based concentrate for enhancing the distribution and permeation of water around and around the root of the plant during watering (Poly) glycocide compound, hereinafter referred to as " APG compound " - and a mixture of it and an olefinically unsaturated fatty alcohol, and an optional Is included as a low water solubility alcohol as a partial ester of a polyhydric alcohol and a fatty acid as a foam inhibitor / defoamer and additionally as a viscosity modifier.

The technical description of the further development described below uses the principles of the above-mentioned prior German patent application. Accordingly, the disclosure of this prior German patent application is specifically incorporated herein as part of the disclosure of the present invention.

The technical description of the above-mentioned patent application is set forth in the following problem: The apparent simplicity of the area of the pavement of the land, more particularly the area of the land densely planted, can cause considerable difficulty. One example of this is the grass area, which is partly walked and / or attached to a means of transportation, where relatively small, even relatively large, grasses can dry out despite regular watering. If an attempt is made to give water to this area, water can not penetrate deeply into the soil, and more particularly, to the root of the grass. The result is that plants within the area can become defective, flat-rooted and malnourished through overdrying. In accordance with the foregoing description of the prior application, the surfactant adjuvant is considered to be an o / w APG compound known to be used on a large scale in different areas as a whole. APG compounds are surface active adjuvants for cleaning agents, especially laundry detergents.

The foregoing description of the prior patent application describes the observation that APG-based aqueous wetting adjuvants are also effective wetting adjuvants of soil irrigation to stimulate plant growth. However, the following problems remain to be addressed in this regard: the APG-based non-ionic surfactants of this type are distinguished by a particularly high foaming property in aqueous formulations. For use in detergents, this is normally welcomed by consumers. However, in the context of the present application to which this invention pertains, this is not only undesirable, but may even be distinctly disadvantageous. While typical bubble-inhibitor additions are known in the area of detergents, the present invention is inadequate to promote related applications, i.e., healthy plant growth. The technical description of the above-mentioned prior patent application uses a selected defoamer or foam inhibitor which, in combination with an APG-based surfactant component, substantially promotes plant growth rather than interferes with it. The selected defoamer is a partial ester of the fatty alcohol and / or lower polyhydric alcohol and the fatty acid mentioned above.

Another problem presented by the description of the prior patent application is that the multicomponent mixture of interest can be constituted in the form of a concentrate which can be immediately diluted with water and even the " dispensability " of an appropriate concentrate has been identified even at room temperature. The technical solution provided by the description according to the prior patent application is that the o / w-type APG compound together with the selected bubble inhibitor of the type mentioned in the aqueous concentrate whose viscosity is controlled through the use of a limited amount of lower water- In the above-mentioned multi-component mixture.

Further developments in accordance with the present invention described below are described in the following additional observations: The APG-based wetting adjuvant of this type not only promotes the permeation of irrigation water into the soil structure, above all, into the root of the plant, Multicomponent aqueous mixtures introduced into and / or applied to the upper parts of plants exhibit unexpected plant-enhancing and / or plant-regenerating effects against plant infections and / or soil pests by phytopathogenic fungi. Areas of soil fungal populations associated with plant roots, and plant-growth-promoting fungi from the mycotic region, are unaffected. Conversely, the multicomponent combination according to the invention produces a plant-growth-stimulating effect in the mycotic and / or rhizosphere regions, which is reflected in the promotion of metabolism and subsequent initiation of plant growth. This is most of all accompanied by the enhancement of healthy plant growth by increased resistance to soil pests, more particularly insects and / or nematodes. However, without any requirement for a complete description of this effect, the additional factor is a useful carbon source, such as a foam inhibitor as described in the partial ester of fatty alcohols and / or fatty acids introduced into the soil with a surfactant APG component, It is involved in the lifespan of the plant, which is controlled by various microorganisms, and also indirectly enhances the plant's resistance and resistance to phytopathogenic fungal infections.

EP 0 230 598 B1 describes the use of alkyl glycoside compounds as agents for controlling harmful organisms in compositions for protecting crops and ornamental plants. For the purposes of the present invention, in addition to the appropriate APG components, the APG compounds listed in the above document include a very broad range of APG compounds, more particularly corresponding to APG ethoxylates. More particularly, the document describes the use of aqueous formulations of APG compounds for plant infections by leaf lice, red spiders and mildew. The present invention is related, more particularly to the use of a surfactant APG component that is in mixture with fatty alcohols and / or fatty acid partial esters within the soil and plant roots, and is of the APG-based Multicomponent mixtures are not disclosed in EP 0 230 598 B1 and there is no reference to the problem of unwanted effervescence or to the technical solution provided by the description of the present invention.

[Subject of the Invention]

Accordingly, the present invention relates to the use of an aqueous formulation as a plant-fortified and / or plant-regeneration mixture (multi-component mixture) effective against infection of plants by phytopathogenic fungi and / or soil pests:

- an ecologically safe surfactant compound (APG compound) from an alkyl (poly) glycoside family of the o / w type and

- partial esters of fatty alcohols and / or lower polyhydric alcohols with fatty acids.

In accordance with the teachings of the present invention, multicomponent mixtures of the type defined herein are used, inter alia, in commercial plant manufacture, landscaping and commercial or domestic horticulture.

As defined in accordance with the present invention, a description of each component of the multicomponent mixture used is given below.

o / w type APG compound

Reference is made firstly to APG compounds, more particularly to the literature on the nature and preparation of APG compounds of the type to which the invention relates, [Hill et al., "Alkyl Polyglycosides", VCH-Verlagsgesellschaft mbH, Weinheim 1997] and extensive scientific knowledge. EP 0 230 598 B1 also provides detailed information on the nature and manufacture of APG compounds.

Preferred APG compounds for the present invention are characterized in that the alkyl (oligo) glucoside compounds in which the alkyl group is derived at least predominantly from straight chain fatty alcohols are at least partially, more particularly at least mainly used. Compounds of this type are surface active adjuvants used for a wide range of applications. Many factors are important for its use on industrial scale. It is known that APG-based wetting agents can be described entirely in natural materials. This is obtained as a product of the reaction of mono-, oligo- and / or poly-saccharides with fatty alcohols. When polysaccharides and / or higher oligosaccharides are used with fatty alcohols, the depolymerization is initiated by hydrolysis and / or alcoholysis during the acid-catalyzed reaction before the desired APG compound is first produced. Preferred saccharide components for the formation of APG compounds are glucose and the corresponding oligo- and poly-glucose. However, other suitable reactants are saccharides based on mannose, galactose, arabinose and other compatible mono-, oligo- and / or multi-saccharides.

A suitable form of APG compound for the present invention is a compound of the formula RO- (G) _{x wherein} R is a primary, preferably at least 6 carbon atoms, preferably 8 to 24 carbon atoms, G is a straight chain aliphatic hydrocarbon radical comprising 18 carbon atoms and G is a glycosose unit comprising 5 or 6 carbon atoms, preferably glucose. In the family of surfactants to which the present invention is concerned, the so-called DP value, which indicates the degree of oligomerization x and the distribution of monoglycosides and oligoglycosides, is usually in the range of 1 to 10, such as about 1.2 to 5, A number of 1.2 to 4, more preferably a number of about 1.2 to 2.

The surfactant component of the mentioned type capable of forming an o / w type APG compound, i.e. oil-in-water emulsion, is distinguished by a relatively high HLB value, i.e. an HLB value of more than 7, preferably 8 or more than 9 And is particularly important when the HLB value is in the range of 10 to 18. [

The fatty alcohols

As mentioned above, there is a multifunctional importance to the above components of the multicomponent mixture used in the present invention. On the one hand, it is effective as a foam inhibitor in practical applications of multicomponent mixtures in water-diluted preparations and, on the other hand, when used in microbial growth, more particularly in the soil, more particularly in the vicinity of plant roots, Lt; / RTI > Fatty alcohols can be degraded aerobically and anaerobically by natural processes. As a crucial carbon source for organic nutritional growth in the present invention, they represent lipophilic lipophilic hydrocarbon radicals and a relatively high concentration of energy-conferring C-H groups within the molecular structure.

In the present invention, preferred fatty alcohols in the multicomponent mixture are monoolefinic and / or polyolefinic unsaturated fatty alcohols, distinguished by at least 6 to 8 carbon atoms in the molecule and containing 10 to 28 carbon atoms, more particularly Particularly preferred are the corresponding fatty alcohols containing from 12 to 24 carbon atoms. Another desirable parameter for the selection of a suitable fatty alcohol is within the solidification range of the component in the multi-component mixture. Corresponding components having a solidification range of 20 DEG C or less, more particularly 10 to 15 DEG C or less, are preferred.

Although it is preferred in the present invention to use fatty alcohols described in the natural materials of the type discussed herein, the description according to the present invention is not limited to these fatty alcohols. Fatty alcohols of synthetic origin, which may even have side chains, are also suitable mixture ingredients in the description according to the invention. In particular, the setting of the solidification range of the alcohol component (s) can be effected in this manner.

Suitable fatty acid moieties esters for the invention

In addition to and / or instead of the fatty alcohols defined above, the fatty acid partial esters of the lower monohydric alcohols may also be important component mixtures in the description according to the invention. In view of the lower polyhydric alcohols, corresponding compounds comprising in particular from 2 to 6 carbon atoms, more particularly from 3 to 5 carbon atoms, are suitable. The corresponding glycerol partial esters are of particular importance, with the merit of immediate accessibility as a natural substance in connection with this. The fatty acids in the family of the partial esters of interest correspond generally to corresponding compounds containing from 10 to 24 carbon atoms and the corresponding C _12-20 monocarboxylic acids are particularly suitable. The fatty acids of this type are known to be commercially available on a large scale as starting materials from natural sources. In this case, it may also be particularly advantageous to use monoolefinic and / or polyolefinic unsaturated fatty acids. A particularly suitable family of active substances is the partial esters of olefinically unsaturated C _16/18 monocarboxylic acids and glycerol, and the corresponding monoesters are also particularly important. This type of fatty acid ester, which is technically feasible on a large scale, is glycerol monooleate.

In the same manner as the above-described fatty alcohols, the fatty acid esters having the constitution described herein can be decomposed in the aerobic and anaerobic conditions in the soil by natural processes. In addition, the material is an important carbon source for organic nutrient growth of microorganisms adjacent to plant roots. Its use also indirectly enhances plant growth by stimulating soil microbial growth.

DETAILED DESCRIPTION OF THE INVENTION [

In the present invention, it is possible to use only the fatty alcohol or only the fatty acid partial ester as the mixture component together with the APG compound. However, one embodiment is characterized by the combined use of fatty alcohols and fatty acid partial esters. The preferred mixing ratio, expressed in parts by weight of the anhydrous components, is in the range of about 1: 1 to 1:10. Fatty alcohol and fatty acid partial esters are preferably mixed at a ratio of 1: 1 to 1: 5 (parts by weight of anhydrous components), more preferably in a ratio of 1: 1 to 1: 3.

In the multi-component mixture used according to the invention, the APG component is used in an amount of at least substantially the same amount as the weight of the fatty alcohol and / or partial ester base, and the APG for the fatty alcohol and / or the partial ester is 1: 1 to 5 : 1, preferably 1: 1 to 3: 1, more preferably 1.5 to 2.5: 1. This figure represents the parts by weight of the mixture described in the dry mix component (s).

However, the description according to the present invention is not limited to the use of an APG component in excess. Mixtures with corresponding excess fatty alcohols and / or fatty acid partial esters used as foam inhibitors are also included within the scope of the description according to the invention.

In the above mentioned German patent application 197 01 127.6, multicomponent mixtures of the type in which the invention is concerned are commercially available and are supplied to the consumer, usually in the form of a liquid aqueous concentrate. The user then dilutes the concentrate that is needed with a larger amount of water to apply the multicomponent mixture to the soil and plant. To ensure that the multicomponent mixture is immediately in the form of a dilutable concentrate with water, its proper "dispensability" must be ensured even at room temperature. The abovementioned description of the prior application discloses that when the aqueous APG concentrate is mixed with a foam inhibitor / defoamer based on the fatty alcohols and / or partial esters mentioned above, a thickened gel, i.e. a gel which no longer flows freely, Clearly. Thus, it has been proposed in the previous application to ensure even more room-temperature addition of a lower amount of monohydric alcohol, more particularly by adding a limited amount of ethanol, to ensure fluidity and effluxability. In connection with the use according to the invention this applies equally to the mixture. Preferred lower polyhydric alcohols are the corresponding compounds containing up to 4 carbon atoms.

In one important embodiment, the description according to the invention is characterized in that a lower monovalent alcohol and, more particularly, a lower polyhydric alcohol which is fluid at room temperature, is used as a mixture component in addition to the above-mentioned components of the multicomponent mixture. Also, in the multicomponent mixture, the polyhydric alcohol has multifunctional significance. As a liquid mixture component having a relatively high melting point, this particularly facilitates the flowability required for handling the concentrate and the possibility of distributing the concentrate; On the other hand, these components - after introduction into the soil - above all, act as nutrients in the rhizosphere and / or in the mycorrhaphy region, which are in the form of additional carbon sources for microbial growth. Preferred polyhydric alcohols are the corresponding compounds containing 2 to 6 carbon atoms, preferably 2 to 4 carbon atoms, and glycerol and / or glycol are particularly important. The solid solubility of the components in water may also be useful for practical application of a multi-component mixture. The preferred amount for the mixture components discussed here is usually in the range of at most 30 to 35% by weight, preferably at most 20 to 25% by weight, more preferably at 5 to 15% by weight The weight percentage of the multi-component mixture substrate in the form of the above-mentioned concentrate, which is diluted with water.

In addition to or in place of the unsaturated fatty alcohol described above as a component of the mixture, olefinically unsaturated terpene alcohols are also suitable as foaming inhibitors / defoamers. Terpene alcohols are non-cyclic or monocyclic, cyclic or triglyceride, polyolefinically unsaturated alcohols of plant origin containing from 10 to 40 carbon atoms. The terpene alcohol is preferably used in the form of its naturally occurring mixture as a foam inhibitor / defoamer. Representatively, [Rompp, Chemielexikon, p. 3451, Vol. 4, 9th revision 1991], which is a mixture of various terpineols, for example a mixture of? - and? -Terpineol,? -Pentyl alcohol, borneol and isoborneyol. Pine oil contains minor amounts of other non-alcohol compounds such as camphor, anethole and estragol. Pine oil is obtained from root wood and resin-containing stubs of various fine species, for example, by extraction with white oil or chloroform, followed by fractionation and distillation.

The above-mentioned defoamer components are usually present in smaller amounts than the APG component based on the active material. Mixtures in which the defoamer component can be used in significantly lesser amounts are preferred. Accordingly, a multi-component concentrate having a defoamer content of about 10 to 25% by weight, preferably about 15 to 20% by weight, is suitable for the present invention.

The lower water-soluble monohydric alcohols used as a viscosity modifier are determined in the form and amount by the total amount of water present in the multi-component mixture, the optional low-water-soluble polyhydric alcohol and the main ingredients mentioned above. Addition of a viscosity regulator of about 5 to 7 wt% or more as a lower limit and 12 to 25 wt% or more as an upper limit is particularly suitable. The addition of about 5 to 20% by weight, more particularly 10 to 15% by weight, of ethanol has a sufficient influence on the viscosity in the direction of the desired flowability and exudability of the multi-component mixture, even at room temperature.

Finally, another embodiment of the present invention is characterized in that the active ingredient or a mixture thereof, selected from the field of phosphorus and / or nitrogen containing fertilizers, is introduced into the top part of the substrate and / or the plant to be treated, . The components which are the carrier of the two elements are preferably representative of a family of such compounds. If desired, other carriers containing large amounts of - and / or micronutrients for plant growth may be used in this connection.

In one particularly important embodiment of the invention, the lipophilic compound of P and / or N is used as a component containing at least some lipophilic moieties. Particularly preferred representatives of such adjuvants are thus the phospholipids and / or derivatives thereof as described in DE 44 37 313, which is cited earlier as a key representative of such components.

Preferred components of this class (d) are esters of monohydric and / or polyhydric alcohols and phosphoric acids containing lipophilic residues within their molecular structure. Among them, partial esters of the corresponding phosphoric acids are particularly suitable and are usually used in the form of their (partial) salts.

Thus, suitable phosphate esters are partial esters of fatty alcohols that introduce the required lipophilic component into the phosphate ester molecule through the hydrocarbon radical of the fatty alcohol. Especially suitable are partial esters of linear fatty alcohols and phosphoric acids, at least substantially prepared using C _6-10 fatty alcohols and / or lower ethoxylates thereof. However, basically, phosphoric esters of higher fatty alcohols, for example containing from 12 to 24 carbon atoms, are also suitable and in this connection the corresponding olefinically unsaturated fatty alcohol radicals are of particular importance.

However, particularly preferred phosphoric esters of this lower series are phospholipids and phospholipid derivatives. These are both affinity materials obtained from plant or animal cells. In the present invention, preferred phospholipids are the corresponding compounds of plant origin and the resulting phospholipid derivatives. A particularly preferred representative of the series (a) is a sugar phospholipid, also commonly referred to as lecithin. Sphingolipids are less desirable. Suitable known materials are diacyl phospholipids, phosphatidyl choline, phosphatidyl ethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylglycerol, phosphatidylglycerophosphate, diphosphatidylglycerol, N-acylphosphatidylethanolamine and phosphatidic acid. Monoacyl phospholipids, lysophosphatidyl choline, lysophosphatidyl ethanolamine, lysophosphatidylinositol, lysophosphatidylserine, lysophosphatidylglycerol, lysophosphatidylglycerophosphate, lysophosphatidylglycerol, lys-n-acyl phosphatidylethanolamine and lysophosphatidic acid. Phosphatidyl glycerides, commercially available as plant or animal lecithin and cephalins, are available on an industrial scale and are available in large quantities. Such formulations are obtained, for example, from oils such as corn oil or cottonseed oil or soybean oil.

Other preferred ingredients are lecithin, lecithin hydrolyzate and / or chemically modified lecithin. If the multicomponent mixture is applied to the top part of the plant, more particularly the leaves, additional N components are preferably not used, but these components may also be used in combination with other N-containing components.

The water content of the multicomponent concentrate is typically less than or equal to about 50 weight percent, assuming a lower value in the preferred embodiment. Thus, a maximum value of about 30 to 45% by weight is particularly suitable for the water content of the multicomponent mixture. Usually, the water content of the multicomponent mixture is much lower, for example in the range of 10 to 20% by weight, preferably 10 to 15% by weight.

In practice, the multicomponent mixtures are applied to soils and / or plants treated in water-diluted form in an amount of at least about 0.5 g / m ² , preferably at least 1 g / m ² , as described in the anhydrous mixture. An application amount of 0.5 to 35 g / m ² is particularly suitable and an application amount of 1 to 20 g / m ² is particularly suitable (in particular an application amount of the APG compound, fatty alcohol and / or fatty acid partial ester, in an anhydrous multi- . The preferred upper limit of application is in the range of 10 to 15 g / m < ² >.

In practice, the concentrate described above is diluted with more water and then finely sprayed, preferably in the form of an aqueous emulsion / dispersion. It may be advantageous in this connection to use a mixture diluted with water having a weight ratio of the multi-component mixture to water of at least 1:10, preferably at least 1:15 to 20 or more.

On an actual level, the description according to the present invention, on the one hand, is intended to prevent the infection by unwanted phytopathogenic fungi populations, on the other hand to protect against soil pests such as unwanted insects and, To give a possibility that is not yet known. In contrast to the usual control of the pests ever made by selected synthetic preparations, which are often not related to the molecules of the natural substance base, the present invention relates to an active-substance mixture whose molecular structure is broadly, use. In this regard, the desired action mechanism is to influence, in particular, the resistance potential of the plant, and more particularly to the metabolic process in the root zone and to the microbial population in the soil associated with the life course of the plant It is indirect reinforcement of the plant by zooming.

The description in accordance with the present invention also includes the important features described below and the actual level of effectiveness in stimulating soil microbial growth: the introduction of the multicomponent mixture according to the invention into the soil is a measurable And an increase in the amount of phosphate available for plant growth. Strengthening and growth of soil microbial guns also obviously result in reinforcement of the aspect of plants in the plant roots. The second effect of increasing root growth is very pronounced in, for example, the grass field with uneven growth density. The supply of roots-available phosphorus to the affected zone more particularly results in a more horizontal spread of the grass roots and an expansion of the roots, especially to the area of the soil without grass, and thereafter, Lt; RTI ID = 0.0 > and / or < / RTI > These effects are disclosed by using multi-component formulations according to the present invention. More particularly, the spread of root growth can rapidly appear as a primary consequence of plant fortification. In this regard, the measurement of the soluble phosphate in the area of the affected soil indicates an increase in the phosphate component already described. This is another initial effect that is fundamental to achieving the object of the present invention, namely plant fortification and / or plant regeneration - not yet known in this form.

The following examples illustrate selected embodiments of the disclosure in accordance with the present invention.

A comparative study in which the development and promotion of the growth of salad plants in a window box filled with standard soil was measured under the same working conditions and the results obtained are reported in the following examples. More particularly, the presence of a multicomponent mixture according to the invention as described in the partial esters of fatty alcohols and / or fatty acids which are in mixture with o / w type APG compounds and the dependence of plant growth under standard conditions at specific concentrations were determined. The effect of using various amounts of conventional plasticizers was further measured in comparative tests.

An aqueous formulation of the product marketed by Henkel KGaA under the trade name Magic Wet at various standard concentrations was used as the o / w type APG compound as a mixture with the multicomponent mixture-fatty alcohol and / or fatty acid partial esters according to the invention. A liquid fertilizer commercially available as Substral (trade name), which is characterized in that the ratio of N to P ₂ O ₅ to K ₂ O is 6: 2: 4 when the usual fertilizer is additionally used in the comparative test. Details of the active substances and auxiliaries of the particular concentration used are given in the following examples.

Materials and methods of comparative growth tests

A 6.7-liter window box with a surface area of 0.06 m ² was used as the test vessel. Lettuce (Lamb's lettuce) seeds were seeded in a window box filled with standard soil and incubated for 8 weeks until ready to be harvested. During this period, the window box was supplied with a specific test solution at regular intervals (1 time before sowing, 9 times after sowing), and only water was given for only four different cases.

Four different dilutions of Magic Wet as shown in the following table were used as test solutions. Within each diluted Magic Wet solution, three different levels of fertilizer were tested:

a) Does not give fertilizer (Mutation types 3, 6, 9, 12, 15)

b) 15 g Substral long-term fertilizer per box (variants 2, 5, 8, 11, 14)

c) 30 g Substral long-term fertilizer per box (variants 1, 4, 7, 10, 13)

The various tests mentioned (mutants) yielded the results shown in Table 1 for the use of water-diluted Magic Wet solutions.

Variant product Dilution Total amount per window box (0.06 m ² ) during the test period 1, 2, 3 Magic Wet 1.0% 27.5 g 4, 5, 6 Magic Wet 0.5% 13.75 g 7, 8, 9 Magic Wet 0.25% 6.88 g 10, 11, 12 Magic Wet 0.125% 3.44 g 13, 14, 15 water - -

The first test was performed in December (Test I) and was repeated in February (Test II) according to the following conditions: The highest concentration of Magic Wet when water was poured on variants 1, 2 and 3 was too high, No information on any practical relevance could be obtained. Therefore, the growth test using this highest concentration was not continued.

During the testing process, the following generally valid observations were made:

In Test I, plants not treated with Magic Wet, in particular, exhibited very high failure rates, which could be due to excessive water cycles. Melting was observed in the corresponding plants of variants 13, 14 and 15, that is, plants not treated with Magic Wet were affected by the corresponding foliar fungus. In contrast, a low concentration of Magic Wet was added, and in other respects fungal infection was prevented in mutants 4-12 through the same water cycle and growth conditions.

The following observations apply to the plant yield of the test used in Test I:

In all tests, five individual but equally treated window boxes were assigned to specific variants (n = 5). The highest fertilizer level (30 g Substral per box) produced comparatively lower biomass (= yield) in Test I than the non fertilizer variant and fertilizer applied to 15 g Substral. Average biomass is shown in Table 2 below.

Variant Magic Wet dilution n Fertilizer Biomass 5 0.5% 5 15 g 42.4 g 6 0.5% 5 - 35.8 g 8 0.25% 5 15 g 43.5 g 9 0.25% 5 - 43.9 g 11 0.125% 5 15 g 41.5 g 12 0.125% 5 - 42.6 g 14 0 4 15 g 31.0 g 15 0 5 - 33.3 g

Variants 14 and 15, untreated with Magic Wet, produced an average of 31 to 33 grams of biomass. By comparison, variants treated with Magic Wet (more particularly 5, 8, 9, 11, 12) produced about 10 g higher biomass. At the same time, there was no significant difference between the 0.5%, 0.25%, and 0.125% Magic Wet concentration levels.

Also in Test II, treatment with 0.5%, 0.25% and 0.125% diluted solutions of Magic Wet (% = weight percentage) produced higher biomass than the untreated control. Table 3 below lists the beneficial data of Test Series II. Substral was used again as a "fertilizer" with a volume of 30 g and 15 g, or not at all. In all comparative test series, the biomass that can be obtained using the Magic Wet is clearly larger than the equivalent test when the Magic Wet is not used. This is especially evident when the greatest amount of Substral fertilizer is used (30 g). This basically a large amount of fertilizer is clearly more resistant to plants through the use of Magic Wet; Expected salt damage is buffered.

Table 3 below contains particularly interesting additional information.

In Tests 13 'and 14', the Magic Wet with the multicomponent mixture was replaced by a combinable amount of o / w pure APG compound. In other words, the experiment was carried out without additional carbon source used in the Magic Wet for bacterial growth. A comparison of the biomass yields obtained in tests 13 'and 14' with the concomitant variants of tests 8 'and 9' shows a pronounced reduction in the biomass yield. In fact, the yields obtained in tests 13 'and 14' are much lower than the biomass yield of test 10 ', ie comparative tests using the same amount of Substral fertilizer without Magic Wet. The plant enrichment and irritation effects of Magie Wet can clearly be attributed to the entire mixture, not just the APG component.

Variant Magic Wet dilution n Fertilizer Biomass 3 ' 0.25 5 30 g 15.8 g 4' 0.125 5 30 g 8.7 g 5 ' 0 5 30 g 5.7 g 16 0.5 5 - 36.8 g 17 0.25 5 - 41.5 g 19 0 5 - 35.6 g 8' 0.25 5 15 g 37.4 g 9 ' 0.125 5 15 g 39.0 g 10 ' 0 5 15 g 28.1 g 13 ' 0.114% APG (= 0.25% MW) 5 15 g 26.4 g 14 ' 0.057% APG (= 0.125%) 5 15 g 19.6 g

Example 2.1

A standard grass mixture (80% Festuca rubra and DSV 414: 20 g / m ² with 20% Agrostis stolonifera) for golf greens was applied to 20% top soil and 80% sand the sprinkled on standard forage based layer with filled tray 144 cm ² (0.2 mm). During the 8-week incubation period in the greenhouse, the herb was fertilized 5 times with liquid fertilizer and cut 3 times (including measurements of fresh and dry weight). After cutting, the treatment of the mixture according to the invention marketed by the present applicant under the trade name " Magic Wet ", together with the application of liquid fertilizer (0.5% NPK 12-4-6 solution) (5th week, 7th week, 8th week). After an 8-week incubation period, the herbs were transferred to a conditioning chamber (10 캜, light 14/10). After 1 day of acclimation, the herbicide was inoculated by overlaying three separate dilute mixtures of Microdochium nivale, the causative agent of eye mold. The grass covered with filter paper was then placed in a conditioning chamber for 8 days under plastic film. Thereafter, evaluation was conducted by visually examining whether or not an eye mold was infected. Until the end, the amount of fungal mycelium visible as a percentage of the per-tray area was evaluated.

All of these mutations were performed 10 times:

Variant density The total concentration Control = water - - Magic Wet 1 g / m ² 3 g / m ² Magic Wet 2 g / m ² 6 g / m ²

In the case of the control mutant type, the average infection of the grass by the mycelial fungi was 78%. These values were reduced by more than half in the Magic Wet treated tray (see Table 1). In addition to this health-sustaining effect of the mixture according to the invention, there was also an indication of growth stimulation reflected, for example, in the 2 g / m ² variant, to 21% higher fresh weight of grass.

Variant Fungal infection Average fresh weight (g / trays) Average dry weight (g / trays) Control = water 78% 0.80 0.19 Magic Wet 1 g / m ² 35% 0.91 0.20 Magic Wet 2 g / m ² 36% 0.97 0.21

The mixture according to the invention is suitable for preventive use to protect golf green against excessive fungal infections.

Example 2.2

A standard grass mixture (DSV 414: 20 g / m ² ) for golf greens was sprayed onto a 144 cm ² tray (0.2 mm) filled with a standard grass substrate layer of 20% top soil and 80% sand. During the 7-week incubation period in the greenhouse, the herb was fertilized 5 times with liquid fertilizer and cut 3 times (including measurements of fresh and dry weight). After 8 weeks of incubation, the eye mold mixture was inoculated with trachea. After 10 days of inoculation, the grasses were sprayed in various ways. On the 14th day after application, the effects of various treatments were visually examined and evaluated. Until the end, the amount of fungal mycelium visible as a percentage of the per-tray area was evaluated.

The following treatments were carried out all 10 times:

Amount of Derosal applied Magic Wet 100% = 18 mg / m < ² > - 75% - 50% - 75% 2 g / m ² 50% 2 g / m ² Control = water -

As expected, after treatment with a standard dose of bactericide, a small amount of mycelium was obtained. However, even the fungicide could not completely inhibit the infection (25% of the trough area affected) (Table 4). Reductions in bactericides resulted in larger infections. Addition of "Magic Wet" 2 g / m ² resulted in an improvement of the effectiveness of the fungicide. For example, using 75% of the usual fungicide dose alone, the fungal infection was 40%, while the Magic Wet was only 35% when used with a fungicide. Also, the presence of Magic Wet had an advantageous effect on biomass.

Variant Fungal infection Average fresh weight (g / trays) Average dry weight (g / trays) Control = water 80% 4.0 0.82 100% disinfectant 25% 4.1 0.87 75% disinfectant 40% 4.1 0.86 75% Fungicide + Magic Wet 2 g / m ² 35% 4.3 0.88 50% disinfectant 50% 4.1 0.85 50% disinfectant + Magic Wet 2 g / m ² 45% 4.2 0.88

As shown in the preceding examples, the mixtures according to the present invention support the therapeutic effect of a bactericide (Derosal in the example), and thus can be used for the treatment of diseases such as, for example, Drechslera, Pythium or Microdochium ) Species, especially where resistance is expected to be enhanced. Therefore, the mixture according to the present invention broadens the range of application of conventional bactericides.

Above all, the amount by which a conventional bactericide is used is reduced by the mixture according to the invention, so that a very serious effect known to have a bactericide for water, soil and habitat can be limited. In addition, a reduction in the amount in which the claimed conventional disinfectant is used can be equalized with an increase in the time interval between the two applications. This reduces the risk of harmful fungi that further enhances resistance.

The mixture according to the invention differs from conventional disinfectants in both its fast and complete biodegradability and overall different mechanism of action. Whereas conventional disinfectants act directly on the metabolism of fungi, the mixtures according to the invention are not understood in detail yet, but they cause changes in the environment. Thus, the protection of cultivated plants against fungi and soil waters through the combined use of conventional biocides and mixtures according to the invention is based on two different but complementary principles.

Example 3

The laboratory-isolated and purified herbicide pathogen was inoculated on four agar test plates and the mycelial growth was measured after incubation for 5 days at room temperature. Nutrient medium of PDA was used. After autoclaving, the mixture according to the invention or the respective components were added at various concentrations after sterile filtration. Agar plates without active material (i.e., only PDA medium) were used as controls. Plants with the usual fungicides Derosal and Folicur at concentrations of 10 ppm and 100 ppm were included in the study, as many bactericides have been available over the years to cause resistance pathogens.

Component 1 = Glucopon 215 45.4 wt%

Component 2 = HD-Ocenol 80/85 4.9 wt%

Component 3 = Edenor GMO 14.6 wt%

result :

Pathogen: Pythium ultimum

The mixtures according to the invention are suitable for inhibiting fungi (P. vestii) causing root rot in growing (Table 5). Since all three components tested are related to the inhibitory effect, the effect appears from the whole mixture. While Folicur is limited in its effectiveness, the fungicide Derosal * is ineffective against fungi.

Pythium Ulthomorphic variant Average radial mycelial growth (mm) Decrease with respect to the control group Control = water 54.5 - Derosal 10 ppm * 54.3 - Derosal 100 ppm * 51.7 5% Folicol 10 ppm 42.3 22% Folicol 100 ppm 0.0 100% Magic Wet 150 ppm 31.0 43% Component 1 100 ppm 35 35% Component 2 100 ppm 54 - Component 2 5000 ppm 31 43% Component 3 100 ppm 54 - Component 3 5000 ppm 35 35%

Pathogen: Microdochium nivale

The mixtures according to the invention are suitable for inhibiting fungi (micrococcioliellae) which cause snow mold during growth (see Table 6: Magic Wet 150 ppm = 2 g / m ² : isolate "Nor77 "50% inhibition). Since all three components tested are related to the inhibitory effect, the effect appears from the whole mixture.

Mikrodo Kiumini Ballet Average radial mycelium growth (mm) Decrease with respect to the control group Variant Isolate Nor77 Isolate Nor2 Isolate Nor77 Isolate Nor2 Control = water 24.3 26.5 - - Component 1 100 ppm 29.8 22.0 - 17% Component 2 5000 ppm 12.5 11.8 48% 44% Component 2 100 ppm 17.8 23.7 27% 10% Component 2 5000 ppm 15.9 20.3 34% 23% Component 3 10 ppm 21.3 22.3 12% 16% Component 3 100 ppm 23.8 24.0 2 % 9%

The present invention is particularly important in the case of isolates with resistance to the conventional bactericides described herein, for example: two of four isolates from the micrococciomyum valleys (Table 6).

Pathogen: Laetisaria fuciformis (Laetisaria fuciformis)

The mixtures according to the invention are suitable for inhibiting pathogens (Laetitia pseudomorphs) which cause red threads during growth (Table 7). Since all three components tested are related to the inhibitory effect, the effect appears from the whole mixture.

Laetitia larvae pseudomorphic variant Average radial mycelium growth (mm) Decrease with respect to the control group Control = water 14.5 Derosal 10 ppm 0.0 100% Derosal 100 ppm 0.0 100% Folicol 10 ppm 0.0 100% Folicol 100 ppm 0.0 100% Magic Wet 150 ppm 8.5 41% Control = water 47.0 - Component 1 10 ppm 41.8 11% Component 1 100 ppm 43.0 8 % Component 2 100 ppm 43.0 8 % Component 2 5000 ppm 32.5 31% Component 3 100 ppm 43.3 8 %

Pathogen: Drechslera dictyiodes

The mixtures according to the invention are suitable for inhibiting pathogens (Drexsleridoticiodes) responsible for leaf blight disease during growth (Table 8). It has been demonstrated that the fungicide Derosal * is less effective than the mixture according to the invention.

Drexslera Dictioides variant Average radial mycelium growth (mm) Decrease with respect to the control group Control = water 19.5 Derosal 10 ppm * 16.8 14% Derosal 100 ppm * 14.3 27% Folicol 10 ppm 0.0 100% Folicol 100 ppm 0.0 100% Magic Wet 10 ppm 19.3 One % Magic Wet 100 ppm 12.0 33%

Pathogen: Drechslera siccans

The mixtures according to the invention are suitable for inhibiting pathogens (Drexlera spp.) Causing leaf blight disease during growth (Table 9). The fungicide Derosal * has proven totally ineffective against this pathogen.

Drake Slaera variant Average radial mycelium growth (mm) Decrease with respect to the control group Control = water 18.3 Derosal 10 ppm * 19.8 - Derosal 100 ppm * 20.0 - Folicol 10 ppm 0.0 100% Folicol 100 ppm 0.0 100% Magic Wet 10 ppm 17.5 4 % Magic Wet 100 ppm 16.5 10%

Pathogen: Drechslera poae (Drechslera poae)

The mixtures according to the invention are suitable for inhibiting pathogens (Drexlerapoa) which cause leaf blight disease during growth (Table 10). While the fungicide Folicur was only effective at high doses, the fungicide Derosal * was ineffective against pathogens.

Variant of Drexslerapoa Average radial mycelium growth (mm) Decrease with respect to the control group Control = water 6.5 - Derosal 10 ppm * 6.5 - Derosal 100 ppm * 6.2 5% Folicol 10 ppm 6.2 5% Folicol 100 ppm 0.0 100% Magic Wet 10 ppm 6.5 - Magic Wet 100 ppm 5.8 11%

As indicated in the preceding examples, the mixtures according to the invention support the therapeutic effect of a bactericide (in the example Derosal) and are particularly resistant to the treatment of diseases such as Drexlera, Pythium or Microcidoum species, Can be used if it is expected to be enhanced. Therefore, the mixture according to the present invention broadens the range of application of conventional bactericides.

Claims (18)

A plant-fortified and / or plant-regeneration mixture (multicomponent mixture) useful for the infestation of plants by phytopathogenic fungi and / or soil pests, the use of an aqueous formulation comprising: - an ecologically safe surfactant compound (APG compound) from an alkyl (poly) glycoside family of the o / w type and - partial esters of fatty alcohols and / or lower polyhydric alcohols with fatty acids. The method according to claim 1, wherein the P and / or N compounds containing at least some lipophilic moieties and, if desired, other large amounts of - and / or micronutrients for plant growth are added to the roots and / Lt; RTI ID = 0.0 > and / or < / RTI > Use according to claims 1 or 2, wherein an APG compound according to at least a substantially saturated C _8-14 head-fractionated fatty alcohol is used and an APG compound which comprises at least predominantly C _8/10 fatty alcohol in the APG molecular structure Lt; / RTI > is preferred. 4. Use according to any one of claims 1 to 3, characterized in that the alkyloligoglucoside is used as the APG compound. 5. A process according to any one of claims 1 to 4, characterized in that it comprises a fatty alcohol containing from 6 to 8 or more carbon atoms in the molecule, preferably from 10 to 28 carbon atoms, more particularly from 12 to 24 carbon atoms Characterized in that mono- and / or polyolefinically unsaturated fatty alcohols are used in the multicomponent mixture. 6. Use according to any one of claims 1 to 5, characterized in that fatty alcohols having a solidification range of 20 DEG C or lower, preferably 10 DEG C to 15 DEG C or lower are used. A fatty acid partial ester of a polyhydric alcohol comprising 2 to 6 carbon atoms, preferably 3 to 5 carbon atoms, more particularly a glycerol partial ester according to any one of claims 1 to 6 For use. 8. A composition according to any one of claims 1 to 7, characterized in that the fatty acid partial esters are derived from _C10-24 , more in particular _C12-20 fatty acids, and monoolefinic and / or polyolefinic unsaturated fatty acids are also preferred Purpose. 9. Use according to any one of claims 1 to 8, characterized in that partial esters of olefinically unsaturated C _16/18 monocarboxylic acids with glycerol, more particularly glycerol monooleate, are used. 10. Use according to any one of claims 1 to 9, characterized in that the fatty alcohols and partial esters, and preferably the APG compounds, are also described in the natural substance. 11. The process according to any one of claims 1 to 10, wherein the fatty alcohol and fatty acid partial esters are used in a ratio of from 1: 1 to 1:10, preferably from 1: 1 to 1: 5, more preferably from 1: 3 (parts by weight of a water-free component). 12. Use according to any one of the preceding claims, wherein the APG component is used in at least substantially equivalent weights as described in fatty alcohols and / or partial esters and the mixing ratio of APG: fatty alcohols and / 1: 1 to 5: 1, preferably 1: 1 to 3: 1, more preferably 1.5: 2.5: 1, in terms of parts by weight based on the weight of the anhydrous mixture. 13. Use according to any one of claims 1 to 12, characterized in that a lower polyhydric alcohol, preferably comprising 2 to 6 carbon atoms, more preferably 2 to 4 carbon atoms, more particularly glycerol and / or glycol Lt; / RTI > to the component mixture. 14. A process according to any one of claims 1 to 13, which is obtainable as an additive viscosity modifier from a flowable concentrate of a lower monohydric alcohol, preferably comprising from 1 to 4 carbon atoms, more particularly a multi-component mixture containing ethanol Characterized in that one dilute aqueous formulation is used. 15. Use according to any one of claims 1 to 14, characterized in that, in addition to or instead of the fatty alcohol, the multi-component mixture comprises terpene alcohol as the mixture component (s). The composition according to any one of claims 1 to 15, characterized in that it has an amount of from 0.5 to 35 g / m ² , preferably from 1 to 20 g / m ² , more preferably from 15 g / m < ² >, preferably in the form of an aqueous emulsion / dispersion, characterized in that a multi-component mixture is applied. 17. Use according to any one of the claims 1 to 16, characterized in that a mixture diluted with water in a weight ratio of the multi-component mixture to water is at least 1: 10, preferably at least 1: 15 to 20, . 18. Use according to any of the claims 1 to 17, characterized in that the multicomponent mixture is used in commercial plant production, landscaping and commercial or domestic horticulture.