CN115226722A - Composition of isothiocyanate and abamectin B2, preparation and application - Google Patents

Abstract

The invention discloses a composition of isothiocyanate and abamectin B2, a preparation and application thereof, wherein the composition comprises the following components in parts by weight: the weight ratio of the isothiocyanate to the abamectin B2 is (1-9) to (9-1). The compound preparation can be prepared into soluble liquid and microcapsule suspending agent, and is applied to the prevention and treatment of soil nematodes. The compound agent has obvious synergy, high nematode killing activity, small relative dosage and obvious fumigation contact killing effect on pests such as soil nematodes and the like.

Description

Composition of isothiocyanate and abamectin B2, preparation and application

Technical Field

The invention belongs to the technical field of pesticide compounding, particularly relates to a pesticide composition for preventing and treating nematode in soil, and particularly relates to a compound agent of isothiocyanate and abamectin B2 and application thereof in preparation of a pesticide fumigant, such as: an isothiocyanate and abamectin B2 composition, a preparation and application.

Background

Isothiocyanates (ITCs) are widely present in cruciferous plants, and particularly in Brassica and Armoracia plants, in appreciable amounts. The content and type of isothiocyanate compounds contained in different plants are different, and most plants contain Allyl Isothiocyanate (AITC), aryl isothiocyanate, propyl isothiocyanate, 4-isopentenyl isothiocyanate, phenyl isothiocyanate, benzyl isothiocyanate, 5-methylthiopentoisothiocyanate, 6-methylthiohexyl isothiocyanate, 7-methylthioheptyl isothiocyanate, etc. Depending on the group of the side chain R, it is classified into three main groups: aliphatic, aromatic, indole. In plant tissues, various types of ITCs coexist, and it is possible that fatty ITCs first exert the biological fumigation effect, and then aromatic ITCs are slowly released to exert the synergistic effect and prolong the effective period. In addition to the flavoring effect, a number of scientific verifications have been made to date for the inhibitory effect of ITCs substances present in cruciferous plants on pathogenic bacteria. The ITCs have strong inhibiting effect on bacteria such as staphylococcus aureus, tubercle bacillus, escherichia coli, helicobacter pylori, bacillus subtilis and the like. It also has high inhibitory rate on fungi such as Candida albicans and Fusarium oxysporum.

Avermectins are sixteen-membered macrolide compounds with insecticidal, acaricidal and nematicidal activities, which were first developed by Dazhou Zhi et al, north Riidy university, and Merck, USA, and are produced by fermentation of Streptomyces griseus (Streptomyces griseus). The natural abamectin contains 8 components, mainly 4 components are A1a, A2a, B1a and B2a, and the total content is more than or equal to 80 percent; the corresponding 4 homologues with smaller proportion are A1B, A2B, B1B and B2B, and the total content is less than or equal to 20 percent. The abamectin B2 has strong contact poisoning and stomach poisoning effects, has a broad spectrum, and has high activity on various crop pests, mites and parasitic nematodes. The abamectin B2 has a unique action mechanism, and is not easy to cause the resistance of pests. The target body of the action is a gamma-aminobutyric acid (GABA) receptor in the peripheral nervous system of the insect. The abamectin B2 can promote the release of gamma-aminobutyric acid from nerve endings, enhance the combination of the gamma-aminobutyric acid and receptors on cell membranes, increase chloride ions entering cells, and hyperpolarize the cell membranes, so that nerve signal transmission is inhibited, and paralysis and death are caused.

CN1124075C discloses a composition containing isothiocyanate and application thereof, which contain AITC and an aldehyde group or ketone group polyol antibacterial composition, and are mainly used for the purposes of treating microorganisms, keeping vegetables fresh and treating perishable goods. CN101133739 has proposed several formulations of products using allyl isothiocyanate as a main active ingredient for soil, food, textile and tobacco warehouses, respectively, and provides a fumigating method very convenient for users, and also indicates the specific application objects and dosages of AITC as a pesticide product.

CN113749090A discloses an abamectin B2 composition, a fermentation process and a preparation method thereof. CN113693074A discloses an acaricidal composition containing abamectin B2, which has a certain synergistic effect on pest mites on crops, and can reduce the usage amount of pesticides, reduce the cost and delay the generation of pathogens and resistance. CN111820229B proposes that the composition of the abamectin B2 and the fluopyram is used for killing nematodes, so that the control effect on root-knot nematodes is greatly improved, and the synergistic effect is more obvious.

In the longitudinal view of the above patents, the avermectin B2 or isothiocyanate is mainly used as the active ingredient for agricultural application or combined with other agents, and is used for preventing and treating common agricultural diseases and pests, and the patents and products for applying the avermectin B2 and isothiocyanate as a compound preparation to prevent and treat recalcitrant soil nematodes are not published and reported so far.

Disclosure of Invention

The invention aims to provide a composition and a compound agent of isothiocyanate and abamectin B2 with obvious synergistic effect.

The invention also aims to provide the application of the compound agent of the isothiocyanate and the abamectin B2 in preparing the pesticide fumigant for preventing and controlling the soil nematodes.

In order to achieve the purpose, the invention adopts the technical scheme that:

the composition of the isothiocyanate and the abamectin B2 is characterized in that the weight ratio of the isothiocyanate to the abamectin B2 in the composition is (1-9) to (9-1).

Optionally, the weight ratio of the isothiocyanate to the abamectin B2 is (4-9) to (3-1).

Optionally, the isothiocyanate is selected from at least one of benzyl isothiocyanate, 2-phenylethyl isothiocyanate and p-trifluoromethylphenyl isothiocyanate.

The composition of the isothiocyanate and the avermectin B2 is characterized in that the isothiocyanate is benzyl isothiocyanate, and the mass ratio of the benzyl isothiocyanate to the avermectin B2 is 7.

The composition of the isothiocyanate and the abamectin B2 is characterized in that the isothiocyanate is benzyl isothiocyanate, 2-phenylethyl isothiocyanate or p-trifluoromethylphenyl isothiocyanate, and the mass ratio of the isothiocyanate to the abamectin B2 is (3-2) to (2-3).

An isothiocyanate and abamectin B2 preparation, which contains the isothiocyanate and abamectin B2 composition.

Optionally, the preparation is a soluble liquid;

the soluble liquid comprises the following components in percentage by weight:

1 to 60 percent of isothiocyanate, 0.2 to 20 percent of abamectin B2, 12 to 60 percent of emulsifier, 1 to 8 percent of ascorbic acid and 100 percent of solvent;

wherein the emulsifier is 500, 602, OP-10, well201, well205 or M30;

the solvent is ethyl acetate or sec-butyl acetate.

Optionally, the preparation is a microcapsule suspending agent;

the microcapsule suspending agent comprises the following components in percentage by weight:

1 to 10 percent of abamectin B, 1 to 20 percent of isothiocyanate, 10 to 20 percent of solvent, 2 to 20 percent of wall material, 2 to 10 percent of emulsifier and 0.1 to 2 percent of thickener; supplementing water to 100%;

wherein the emulsifier is 500, 1601, OP-10, TW-20 or TW-80; the solvent is ethyl acetate, sec-butyl acetate or turpentine; the thickening agent is xanthan gum; the wall material is tetraethoxysilane, formaldehyde or urea.

The composition of isothiocyanate and abamectin B2 is applied to preparing the medicines for preventing and treating soil nematodes.

The isothiocyanate and abamectin B2 preparation is applied to preparing the soil nematode prevention and treatment drug.

The invention has the beneficial effects that:

the invention takes isothiocyanate as a main active ingredient and takes avermectin B2 as a synergistic ingredient; the method is suitable for soil treatment of different occasions such as greenhouse soil, protected land and the like; has nematicidal biological activity; the composition mainly fumigates and kills plant root-knot nematodes, has obvious synergistic effect, reduces the dosage of isothiocyanate, reduces toxicity and is beneficial to environmental protection.

Detailed Description

The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.

The existing nematicide registered products with isothiocyanate as an active ingredient contain 20% of allyl isothiocyanate aqueous emulsion and 20% of soluble liquid respectively, and high-content allyl isothiocyanate is mainly used for soil fumigation or soil film fumigation, but the product can penetrate through polyolefin plastic films such as polyethylene, polypropylene and the like and has strong irritation to the skin and eyes of people in the using process, so that certain limitation is caused to the application of the product. To effectively assist the new product in use, a synergistic combination of fumigation is needed to reduce the content of the preparation. The sixteen-element macrolide compounds with insecticidal, acaricidal and nematicidal activities are generated by fermenting Streptomyces griseus (Streptomyces griseus) in Streptomyces. The abamectin B2 inhibits normal nerve impulse conduction of a target organism by stimulating and releasing rich gamma-aminobutyric acid (GABA) to paralyze, antifeedant and cause death of nematodes.

Because nematodes are hidden in plant roots or soil, their control is particularly difficult, which requires that the agent has strong contact and fumigation activity or good penetration. The isothiocyanate compound has good fumigating activity on nematodes in soil, the abamectin B2 has good contact killing activity on the nematodes, and the combination of the two effectively controls soil-borne pests in different action modes and action mechanisms.

When carrying out an isothiocyanate soil fumigation test, the inventor finds that an isothiocyanate single agent has the defects of limited penetrating capacity, short acting time, easiness in being influenced by soil moisture, easiness in hydrolysis under an acidic condition and the like in use, has abnormal pungent odor, is easy to hurt operators in pesticide application, and necessarily finds that the effect can be remarkably enhanced, the acting time and the acting effect are increased, the prevention and treatment effect is improved, the dosage of the pesticide is reduced, and the environmental pollution is reduced by compounding.

Therefore, through a large number of experiments, the inventor prepares three isothiocyanate single agents of benzyl isothiocyanate, 2-phenylethyl isothiocyanate and p-trifluoromethylphenyl isothiocyanate, and carries out fumigation activity screening on the root-knot nematode.

Abamectin is one of the insecticides, acaricides and parasiticides which have been found to be the most efficient nowadays. The abamectin has strong contact poisoning and stomach poisoning effects, is broad-spectrum, and has high activity on various crop pests, mites and parasitic nematodes. Unlike other chemical pesticides, abamectin B2 inhibits normal nerve impulse conduction of target organisms by stimulating release of abundant gamma-aminobutyric acid (GABA) to paralyze, antifeedant and cause death of nematodes.

The fumigation activity of the isothiocyanate is obvious, but the duration is short, through screening the isothiocyanate synergist, the effect of the composition of the abamectin B2 and the isothiocyanate is obvious compared with that of a single agent, the using amount of the isothiocyanate is reduced, the quick effect is obvious, the duration is long, the composition is more friendly to the environment, is safe to people and livestock, a more ideal fumigation medicament can be formed, and the isothiocyanate and the abamectin B2 are compounded into a new nematicide.

The mechanism of the fumigating action of the athomin (allyl isothiocyanate) on the pests is as follows: the horseradish peroxidase binds to the mitochondrial complexes I and IV and inhibits the activity thereof, resulting in incomplete oxygen reduction, and active oxygen radicals are generated in vivo, and in the case where the CAT and POD activities are inhibited and the SOD activity is increased, it further results in excessive accumulation of oxygen radicals, thereby causing lipid peroxidation of the mitochondrial membrane, and ultimately death.

In order to achieve the purpose of the invention, the ratio screening of two compounds is further carried out to find that the weight ratio of the effective component isothiocyanate to the synergistic component abamectin B2 is (1-9) to (9-1), and the weight ratio of the isothiocyanate to the abamectin B2 is (1-9).

The compound preparation of the isothiocyanate and the abamectin B2 can be prepared into pesticide preparations which are well known in the field, such as soluble liquid, microcapsule suspending agent and the like. Can be made into different dosage forms according to different application occasions.

The soluble liquid comprises the following components in percentage by weight: 1 to 60 percent of isothiocyanate, 0.2 to 20 percent of abamectin B2, 12 to 60 percent of emulsifier, 1 to 8 percent of ascorbic acid and 100 percent of solvent. Wherein the emulsifier is 500, 602, OP-10, well201, well205 or M30 (the four are Fujianwell chemical production); the solvent is ethyl acetate or sec-butyl acetate, wherein a mixed solvent is preferred, and the ratio of ethyl acetate to sec-butyl acetate is 1 (V/V). The preparation process comprises the following steps: firstly, ascorbic acid is put into mixed mother liquor of abamectin B2 and isothiocyanate which are weighed quantitatively and fully and evenly stirred. And fully and uniformly mixing the agricultural emulsifier and the solvent which are weighed accurately and quantitatively. And finally, continuously stirring the two solutions in a preparation tank for 1-3 hours. The product is subpackaged in double-barrier plastic bottles and stored in a sealed way.

The microcapsule suspending agent comprises the following components in percentage by weight: 1 to 10 percent of abamectin B, 1 to 20 percent of isothiocyanate, 10 to 20 percent of solvent, 2 to 20 percent of wall material, 2 to 10 percent of emulsifier and 0.1 to 2 percent of thickener; 0.1 to 2 percent of defoaming agent; and water is replenished to 100 percent. Wherein the emulsifier is 500, 1601, OP-10, TW-20, TW-80; the solvent is ethyl acetate, sec-butyl acetate or turpentine; the thickening agent is xanthan gum; the wall materials are tetraethoxysilane, formaldehyde, urea and the like. The preparation process comprises the following steps: the method comprises the steps of firstly oscillating and uniformly mixing the abamectin B2, the isothiocyanate, the agricultural emulsifier and the solvent which are quantitatively weighed, slowly dripping the mixed solution into a reaction bottle filled with pure water, homogenizing and emulsifying for a certain time at a high speed, and dispersing the mixed solution into small droplets under the action of high-speed shearing. The mixture is homogeneously dispersed in the water solution to form stable O/W emulsion. And (3) dropwise adding a certain amount of ethyl orthosilicate into the emulsion, uniformly stirring, performing ultrasonic treatment for 10min, continuously stirring at room temperature for reacting for 4h, and adding auxiliaries such as a thickening agent and a defoaming agent.

The production process is completed by a machine under a closed condition, and personnel are prevented from directly contacting the liquid medicine.

The composition of isothiocyanate and abamectin B2 is used for preparing a pesticide fumigant for preventing and treating soil nematodes.

The meloidogyne incognita (m.incognita) used in the following experiments was isolated and identified by the laboratory itself.

Experimental example 1:

the three compounds and the compound agent thereof are used for measuring the indoor fumigation activity research of the meloidogyne incognita, and 6 treatments are set in the test;

(1) 18 μ g/mL benzyl isothiocyanate;

(2) 2-phenylethyl isothiocyanate at 18 μ g/mL;

(3) 18 μ g/mL of p-trifluoromethylphenyl isothiocyanate;

(4) 18 μ g/mL benzyl isothiocyanate +2 μ g/mL avermectin B2 (9;

(5) 18 μ g/mL 2-phenylethyl isothiocyanate +2 μ g/mL abamectin B2 (9;

(6) 18 μ g/mL p-trifluoromethylphenyl isothiocyanate +2 μ g/mL avermectin B2 (9;

TABLE 1 Fumigation Activity of different isothiocyanate mono-and combination formulations against Meloidogyne spp biological Activity

Treatment of	24h mortality (%)	48h mortality (%)	Mortality rate (%) at 72h
				1	65.8	73.2	78.5
2	49.6	63.1	72.2
				3	45.2	49.7	66.5
4	72.3	85.1	90.3
				5	60.1	77.0	80.2
6	56.1	73.2	83.9

And (3) determining the fumigation activity of the sample on the nematodes by adopting a fumigation poisoning method. Placing a culture dish filled with a medicament to be tested with a certain concentration on the bottom layer of a closed dryer (the medicament concentration is the medicament volume/the dryer volume), placing a 24-hole plate filled with the nematode to be tested on the upper layer of the dryer, quickly sealing the dryer, placing the dryer in an environment with the temperature of 25 +/-2 ℃ for culturing, taking out the 24-hole plate after 24 hours and 48 hours, and observing the survival condition of the nematode.

Experimental example 2

The isothiocyanate, the abamectin B2 and the compound agent thereof respectively research the indoor biological activity of the root-knot nematode, and 7 treatments are set in the test;

(1) 20 μ g/mL benzyl isothiocyanate;

(2) 20 μ g/mL 2-phenylethyl isothiocyanate;

(3) 20 μ g/mL of p-trifluoromethylphenyl isothiocyanate;

(4) 20 mu g/mL of abamectin B2;

(5) 20 μ g/mL benzyl isothiocyanate +5 μ g/mL avermectin B2 (4;

(6) 20 μ g/mL 2-phenylethyl isothiocyanate +5 μ g/mL abamectin B2 (4;

(7) 20 μ g/mL p-trifluoromethylphenyl isothiocyanate +5 μ g/mL avermectin B2 (4;

TABLE 2 indoor bioactivity of different isothiocyanates single and compound preparations on Meloidogyne spp

Preparing a drug-containing suspension with a certain concentration by a 24-hole disc method, adding the prepared second-instar larvae of the root-knot nematodes, observing and counting the mortality of the nematodes after 24 hours, 48 hours and 72 hours, wherein benzyl isothiocyanate, 2-phenylethyl isothiocyanate and p-trifluoromethylphenyl isothiocyanate have certain contact killing activity on the root-knot nematodes. The abamectin B2 has good contact activity, when the concentration of the abamectin B2 is 20 mu g/mL, the control effect on the meloidogyne incognita for 24h and 48h is 65.6 percent and 77.5 percent respectively, the biological activity of the compound agent is obviously higher than that of a single agent, and the using amount of isothiocyanate can be obviously reduced.

Experimental example 3

And (3) measuring the biological activity of the 2-phenylethyl isothiocyanate and abamectin B2 composition soluble liquid agent on the root-knot nematode.

The 2-phenylethyl isothiocyanate and the abamectin B2 obtained in the method are respectively combined according to different proportions to prepare a soluble liquid agent, and the indoor biological activity of the root-knot nematode is compounded and screened. The method comprises the steps of researching the indoor biological activity determination of the compound medicaments with different proportions on the root-knot nematode by adopting a 24-hole disc method, checking results after 24 hours, and respectively calculating LC (liquid chromatography) of 2-phenylethyl isothiocyanate and LC (liquid chromatography) of abamectin B2 ₅₀ And one of the single doses is set as a standard medicament to be mixed with LC ₅₀ Calculating the toxicity index of each single agent, and further calculating the theoretical toxicity index of the mixed agent. The synergistic effect of the compounded medicament is evaluated according to the co-toxicity coefficient, and the calculation formula is as follows:

the toxicity index of the mixed preparation theory = ∑ single preparation toxicity index x the mass percentage content of the single preparation in the mixed preparation

Note: when CTC is more than or equal to 120, the effect is synergistic; when CTC is less than or equal to 80, the antagonism is obtained; additive effect when 80 < CTC < 120.

TABLE 3 indoor bioactivity of the compounded preparation against root-knot nematodes

From the above table, 2-phenylethyl isothiocyanate, abamectin B2=4, has the best activity against meloidogyne incognita, LC ₅₀ 8.6 mu g/mL, a coefficient of synergy of 192.73, significant synergy, and LC ₅₀ The value is lower, and the practical possibility of application to production is provided.

Experimental example 4

And (3) measuring the biological activity of the benzyl isothiocyanate and abamectin B2 composition microcapsule suspending agent on the root-knot nematode.

The benzyl isothiocyanate and the abamectin B2 obtained in the above way are respectively combined according to different proportions, and are prepared into a microcapsule suspending agent, and the indoor biological activity of the root-knot nematode is compounded and screened. And (3) researching the indoor biological activity determination of the compound medicaments with different proportions on the root-knot nematode by adopting a 24-hole disc method, and checking the result after 24 hours. The cotoxicity coefficient was calculated according to the formula in experimental example 3.

TABLE 4 indoor bioactivity of the compounded preparation against root-knot nematodes

From the above table, it can be seen that benzyl isothiocyanate, abamectin B2=7, has the best activity against meloidogyne incognita, and LC thereof ₅₀ 8.8 mu g/mL, a coefficient of synergy of 208.88, significant synergy, and LC ₅₀ The value is low, and the method has practical application possibility in production.

Experimental example 5

The biological activity of the p-trifluoromethylphenyl isothiocyanate and abamectin B2 composition soluble liquid agent on the root-knot nematode is measured according to the method of the experimental example 3.

TABLE 5 indoor bioactivity of the compounded preparation against root-knot nematodes

From the above table, p-trifluoromethylphenyl isothiocyanate: abamectin B2=9 ₅₀ 10.8 mu g/mL, the synergistic coefficient is 180.64, the obvious synergy is achieved, and LC ₅₀ The value is lower, and the practical possibility of application to production is provided.

Experimental example 6

Potting test of benzyl isothiocyanate and abamectin B2 compound preparation on meloidogyne incognita

Soil collected from a cultivated layer (the depth is about 0-15 cm) of a tomato field with serious root knot nematode disease in the base of a modern agricultural park in Weinan, shanxi province is subjected to heat preservation and moisture preservation, and tomato diseased roots infected with the root knot nematode disease are buried and are allowed to rot in the soil. After a period of time, the soil contains a large number of tomato root-knot nematode larvae. The soil (absolute moisture content about 20%) was then mixed well and loaded into pots, 2kg per pot. Then, the following concentration gradient was followed: 10. 50 and 125 mu L/kg (weight ratio of effective components to soil), respectively burying the compound preparation in a position of about 5cm in a basin, and pouring a small amount of clear water. And finally, sealing the pot and the sand applied with the pesticide for 7 days by using a polyvinyl chloride plastic film, and transplanting the tomato seedlings after opening the pot and the sand for 4 days. Meanwhile, 41.7% fluopyram suspension with a concentration of 40mg/kg was used as a drug control and a treatment without any drug was set as a blank control, and each treatment was repeated 5 times and cultivated under the same water and fertilizer management conditions. The results were counted two months later and the results are as follows.

TABLE 6 potted plant test results of the compound preparation of BITC and avermectin B2 on Meloidogyne incognita

Note: in the table, "B" represents BITC (benzyl isothiocyanate) "and" A "represents avermectin B2.

From the results, the control effects of the single agent of the BITC and the abamectin B2 respectively reach 62% and 65% when 50 mu L/kg of treatment is carried out, and the control effect can reach 65% when the weight ratio of the compound agent of the benzyl isothiocyanate to the abamectin B2 is 7 and 10 mu L/kg of treatment is carried out, so that the control effect of the BITC is obviously improved, and the use amount of the BITC is reduced.

Example 1

And (3) producing a soluble liquid agent line killing A by using the composition of 14% benzyl isothiocyanate and 6% abamectin B2.

175 kg of 40 percent benzyl isothiocyanate extraction mother liquor and 31.25 kg of 96 percent abamectin B2 original drug are accurately and quantitatively weighed. Mixing the two active medicinal liquids in blending tank (1). Then 18 kg of ascorbic acid is accurately weighed and put into the blending tank (1) to be fully and uniformly mixed again. Adding 602 kg of emulsifier and OP-1050 kg of emulsifier into the blending tank (2), mixing, adding 215.75 kg of ethyl acetate, and fully and uniformly mixing. Transferring all the kilogram materials in the blending tank (2) into the blending tank (1) through a pipeline, sealing the opening of the tank, and starting the stirrer to stir for 1 hour. 500 kg of benzyl isothiocyanate soluble liquid product is prepared. The content of benzyl isothiocyanate in the product is 14 percent through sampling analysis, the content of abamectin B2 is 6 percent, and the product A is the line killing product A with the concentration of 20 percent, and the product is subpackaged into double-barrier plastic bottles with 500mL of medicine in each bottle.

Example 2

Production of composition soluble liquid containing 9% of p-trifluoromethyl phenyl isothiocyanate and 1% of abamectin B2 for killing B in line

69.3 kg of 65% p-trifluoromethyl phenyl isothiocyanate extraction mother liquor and 5.2 kg of raw pesticide with the abamectin B2 content of 96% are accurately and quantitatively weighed. Mixing the two active medicinal liquids in blending tank (1). Then accurately weighing 15 kg of ascorbic acid, and putting the ascorbic acid into the blending tank (1) to be fully and uniformly mixed again. Adding 80 kg of TW and 1601 50 kg of emulsifier into the blending tank (2), mixing, adding 350.5 kg of sec-butyl acetate, and fully mixing. Transferring all the kilogram materials in the blending tank (2) into the blending tank (1) through a pipeline, sealing the opening of the tank, and starting the stirrer to stir for 1 hour. 500 kg of p-trifluoromethylphenyl isothiocyanate soluble liquid product is obtained through preparation. The content of the para-trifluoromethylphenyl isothiocyanate in the product is analyzed by sampling to be 9 percent, the content of the avermectin B2 is 1 percent, namely the product B is a 10 percent linear killing product B, and the product is subpackaged into double-barrier plastic bottles, wherein each bottle contains 500mL of the medicine.

Example 3

And 3, producing a microcapsule suspending agent line killing C by 8 percent of 2-phenylethyl isothiocyanate and 2 percent of abamectin B2 composition.

111.1 kg of 2-phenylethyl isothiocyanate extraction mother liquor accounting for 36 percent and 10.4 kg of raw pesticide with the abamectin B2 content accounting for 96 percent are accurately and quantitatively weighed. Mixing the two active medicinal liquids in blending tank (1). Then 50 kg of sec-butyl acetate, 50 kg of TW and 213.5 kg of water are accurately weighed, mixed uniformly and sheared at high speed for 30min. Adding 61 kg of tetraethoxysilane into a blending tank (2), completely transferring the 61 kg of materials in the blending tank (2) into the blending tank (1) through a pipeline, sealing the opening of the tank, starting a stirrer to stir uniformly, performing ultrasonic treatment for 1h, continuing stirring for 4h, adding 5 kg of thickening agent xanthan gum, and preparing to obtain 500 kg of 2-phenylethyl isothiocyanate soluble liquid product. The content of 2-phenylethyl isothiocyanate in the product is analyzed by sampling to be 8 percent, the content of abamectin B2 is 2 percent, namely the microcapsule suspending agent line killing C product with the content of 10 percent is obtained, and the product is subpackaged into plastic bottles with 500mL of medicine in each bottle.

Example 4

And (3) producing a microcapsule suspending agent of 7% benzyl isothiocyanate and 3% abamectin B2 composition by using a liquid agent line killing method D.

175 kg of 40 percent benzyl isothiocyanate extraction mother liquor and 31.25 kg of 96 percent abamectin B2 original drug are accurately and quantitatively weighed. Mixing the two active medicinal liquids in blending tank (1). Then 100 kg of sec-butyl acetate is accurately weighed and put into the blending tank (1) to be fully and uniformly mixed again. Adding 34 kg of emulsifier agricultural emulsion and 50 kg of emulsifier OP-10 kg into a blending tank (2), mixing, adding 618 kg of urea-formaldehyde resin prepolymer, and fully and uniformly mixing. Transferring all kilogram materials in the blending tank (2) into the blending tank (1) through a pipeline, sealing the opening of the tank, and starting a stirrer to stir for 1 hour. And adjusting the pH value to 2 in batches, reducing the rotating speed, heating to 60 ℃, and carrying out curing reaction for 1h. And adjusting the pH value to 7.0 at the end of the reaction, adding 2kg of thickening agent, and adding water to complement to 1000 kg, thus preparing 1000 kg of the benzyl isothiocyanate abamectin capsule suspending agent product. The content of benzyl isothiocyanate in the product is 7 percent and the content of abamectin B2 is 3 percent through sampling analysis, namely the 8 percent microcapsule suspending agent line killing D product is obtained, and the product is subpackaged into plastic bottles, wherein each bottle contains 500mL of medicine.

Example 5

Selecting a field block for planting tomatoes all year round in a Shanxi Weinan vegetable base, seriously harming southern root-knot nematodes for a long time, dividing cells with consistent sizes, wherein the area of each cell is 20m ² The two-dimensional pesticide is randomly arranged, holes with the same size are opened, roots are irrigated in each hole by hole application, the contrast agent is 41.7% fluopyram microcapsule suspending agent (Germany Bayer), the treatment group agent is 20% soluble liquid agent line killing A (14% benzyl isothiocyanate and 6% abamectin B2), the soluble liquid agent line killing A is respectively diluted by 2000 times, the hole application amount is 250ml, and the statistical results after two months show that the control group and 20% soluble line killing control effects are respectively 45% and 83%.

Example 6

Selecting a field block with serious cucumber root-knot nematode infection in a vegetable test base in corridor city of Hebei province, dividing the field block into uniform cells, and randomly arranging the cells in blocks, wherein the area of each cell is 20m ² Protection rows are arranged among the cells and around the test field. Directly pouring water, coating film, fumigating soil for 7 days, and transplanting seedlings at intervals of 14 days. The control agent is 20% fosthiazate aqueous emulsion (Jiangsu Huifeng biological agriculture Co., ltd.), the treatment group agents are 10% soluble liquid agent linekill B (9% para-trifluoromethylphenyl isothiocyanate and 1% avermectin B2), the treatment group agents are respectively diluted by 1200 times, the dosage per square meter of each treatment is 15g, and statistics is carried out after two monthsThe root knot number of each gram of root is 61 percent and 89 percent respectively.

Example 7

The test is carried out in Li Jiazhuang Town of Anqiu city, shandong province, the selected test field is open-air ginger field, the ginger variety is flat top No. 4, seeding is carried out at the bottom of 3 months, and harvesting is carried out at the bottom of 10 months. The experimental field root-knot nematode occurs frequently and has consistent soil fertility. The random block arrangement is that each cell comprises 3 ridges and has an area of about 30m ² (15 m × 2 m), and a guard row is set for each cell. During the test period, the 10% microcapsule suspending agent line killing C produced in example 3 is not adopted, the contrast group adopts three treatment modes of commercial 20% horse radish hormone soluble liquid and clear water, each treatment mode is repeated, the microcapsule suspending agent is diluted by 600 times, the commercial horse radish hormone soluble liquid is diluted by 1200 times, the ditch is filled, the using amount of each ridge is 10L, soil is covered, then a thick film is laid, the ditch is sealed for seven days, and then the ditch is opened for four days, and ginger is planted. Other agents for preventing and controlling nematodes are not used during the test period, and other management is consistent with normal farming operation. And (5) checking the root knot condition in the ginger harvesting season. The control effect of the treatment group and the control group is 89 percent and 76 percent respectively.

Example 8

Soil with serious meloidogyne incognita disease is collected from Dali county in Weinan city, shanxi province and is filled into flowerpots, and 3 kilograms of soil is used in each flowerpot. And (3) filling 100 pots, wherein 50 pots are filled with 250mL of clean water to serve as blank control, and the blank control pots are tightly wrapped by polyvinyl chloride plastic films. Then the 20% soluble liquor produced in example 1 was diluted 1200 times and 250mL of the diluted liquid medicine was filled in each treatment tank, the treatment groups were wrapped up in the same way, sealed for 7 days, the wrapped film was removed, and left to be aerated for 4 days. Then transplanting tomato seedlings, two plants per pot. Tomato seedlings were allowed to grow for 45 days under the same management conditions. The blank control shows that 94 tomato seedlings die from 100 melon seedlings, only 4 tomato seedlings die from 100 tomato seedlings on the soil treated by the pesticide, and the control effect reaches 96%.

Example 9

Soil blocks seriously infected by root-knot nematodes are uniformly mixed and loaded into flowerpots, and each pot is filled with 2kg of soil blocks. Then, the 10% microcapsule suspension produced in example 4 was diluted 300 times with the nematocide D (7% benzyl isothiocyanate +3% avermectin B2), 300ml of the diluted drug was applied to each pot, the pot was buried in a position of about 5cm, the pot to which the drug was applied was sealed with a polyvinyl chloride plastic film for 7D, and after opening 4D, cucumber seeds were sown in the pots, and the blank and the treatment group were 240 seeds. Statistics of the emergence rate after half a month, the control had little survival. The mortality rate of the drug-treated group was only 11.7%.

Although the invention has been described in detail with respect to the general description and the specific embodiments thereof, it will be apparent to those skilled in the art that modifications and improvements can be made based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims (10)

1. The composition of the isothiocyanate and the abamectin B2 is characterized in that the weight ratio of the isothiocyanate to the abamectin B2 in the composition is (1-9) to (9-1).

2. The composition of isothiocyanate and avermectin B2 of claim 1, wherein the weight ratio of isothiocyanate to avermectin B2 is (4-9) to (3-1).

3. The isothiocyanate and avermectin B2 composition of claim 1 or 2, wherein the isothiocyanate is selected from the group consisting of benzyl isothiocyanate, 2-phenylethyl isothiocyanate and p-trifluoromethylphenyl isothiocyanate.

4. The composition of the isothiocyanate and the abamectin B2 is characterized in that the isothiocyanate is benzyl isothiocyanate, and the mass ratio of the benzyl isothiocyanate to the abamectin B2 is 7.

5. The composition of the isothiocyanate and the abamectin B2 is characterized in that the isothiocyanate is benzyl isothiocyanate, 2-phenylethyl isothiocyanate or p-trifluoromethylphenyl isothiocyanate, and the mass ratio of the isothiocyanate to the abamectin B2 is (3-2) to (2-3).

6. An isothiocyanate and abamectin B2 formulation comprising the isothiocyanate and abamectin B2 composition of any one of claims 1 to 5.

7. The isothiocyanate and avermectin B2 formulation of claim 6, wherein said formulation is a soluble liquor;

the soluble liquid comprises the following components in percentage by weight:

1 to 60 percent of isothiocyanate, 0.2 to 20 percent of abamectin B2, 12 to 60 percent of emulsifier, 1 to 8 percent of ascorbic acid and 100 percent of solvent;

wherein the emulsifier is 500, 602, OP-10, well201, well205 or M30;

the solvent is ethyl acetate or sec-butyl acetate.

8. The isothiocyanate and avermectin B2 formulation of claim 6, wherein said formulation is a microcapsule suspension;

the microcapsule suspending agent comprises the following components in percentage by weight:

1 to 10 percent of abamectin B, 1 to 20 percent of isothiocyanate, 10 to 20 percent of solvent, 2 to 20 percent of wall material, 2 to 10 percent of emulsifier and 0.1 to 2 percent of thickener; supplementing water to 100%;

wherein the emulsifier is 500, 1601, OP-10, TW-20 or TW-80; the solvent is ethyl acetate, sec-butyl acetate or turpentine; the thickening agent is xanthan gum; the wall material is tetraethoxysilane, formaldehyde or urea.

9. Use of the isothiocyanate and avermectin B2 composition of any one of claims 1-5 in the preparation of a medicament for controlling soil nematodes.

10. Use of the isothiocyanate and abamectin B2 formulation of any of claims 6 to 8 in the preparation of a medicament for controlling soil nematodes.