KR20010045177A - Microbial activator for using in treating sewage, night soil and industrial waste water and manufacturing method of them - Google Patents

Abstract

PURPOSE: Disclosed is an agent for cultivating microorganism for using in the biological treatment of wastewater. Especially, the agent is effective in cultivating Bacillus. CONSTITUTION: The agent comprises silicic fertilizer, MgSO4·7H2O, and peat in a ratio of 55-65 wt%, 18-22 wt.%, and 18-22 wt.% respectively. Silicon of silicic fertilizer need to the growth of Bacillus. Magnesium of MgSO4·7H2O need to activate Bacillus, and sulfur has vitamin and component of amino acid. Also, peat has much Bacillus, and various minerals for growth of Bacillus are eluted from peat.

Description

Microbial culture agent for the biological treatment of sewage, manure and industrial wastewater and its manufacturing method {MICROBIAL ACTIVATOR FOR USING IN TREATING SEWAGE, NIGHT SOIL AND INDUSTRIAL WASTE WATER AND MANUFACTURING METHOD OF THEM}

The present invention relates to a microbial culture agent and a method for manufacturing the same, and more particularly, to a microbial culture agent for predominantly culturing Bacillus bacteria among microorganisms used to treat sewage, manure and industrial wastewater and the like. It is about.

In general, sewage, manure and industrial waste water (hereinafter referred to as waste water) can be said to be suspended or dissolved by mixing water and pollutants. Thus, the treatment of waste water is a series of processes for separating and removing contaminants mixed in water and suspended or dissolved. The treatment of waste water is to remove contaminants that have a smaller specific gravity and volume than water by physical methods such as filtration or sedimentation, and chemically remove contaminants that are difficult to remove by physical methods. The chemical method uses chemicals to agglomerate fine particles so that contaminants can be removed physically. At this time, the manure includes livestock-related organic waste, industrial wastewater is to include food-related organic waste.

On the other hand, when the particles of contaminants are very fine or dissolved in a dissolved state, and it is difficult to remove them by physical and chemical methods, biological treatment is used to absorb or decompose contaminants using microorganisms. Although, recently, a method for removing such contaminants by filtration such as membrane filtration has been proposed, biological treatment is recognized as the most economical and stable treatment of wastewater. The treatment of waste water can obtain satisfactory treatment water only by the physical and chemical treatment methods depending on the characteristics of the waste water, but most treatment plants require a combination of physical, chemical and biological methods. In particular, the importance of biological treatment methods is increasing as the demand for advanced treatment is increased due to the strengthened water quality standards for effluent from wastewater treatment plants.

Recently, Bacillus bacillus has been known to have excellent effects in the treatment of organic wastewater as well as the removal of nitrogen and phosphorus. Bacillus bacteria are used for the treatment of wastewater, which is a wastewater treatment system implemented by Daekyung Machinery Technology Co., Ltd. under the trade name B-Best-Bacillus System. Bacillus bacteria hydrolyze not only organic substances but also hardly degradable cellulose, proteins, starches and fats, and directly ingest the ammonia, hydrogen sulfide, and amines, which are odors generated when treating wastewater. Decompose to remove odor. Since Bacillus bacteria have endogenous spores, they can adapt to changes in environmental conditions compared to microbial groups that are applied to existing wastewater treatment even if the surrounding environmental conditions change. → Survival is repeated for the same process as spores, so it is possible to maintain high concentration of cells. Since the surface of Bacillus bacteria is covered with a sticky substance, adsorption and aggregation occur well, and the formed floc has good precipitation. In addition, nitrogen or phosphorus contained in the sludge is known to be present in the cells of the spores Bacillus bacteria, there is no fear of eluting in the sedimentation basin or sludge concentration tank.

As such, in order to treat wastewater using Bacillus bacteria that can efficiently treat not only organic substances but also nitrogen and phosphorus, it is most important to allow Bacillus bacteria to become dominant species.

However, in the operation of a general wastewater treatment plant, general bacteria are predominant and dominated by Bacillus bacteria. Therefore, it is difficult to cultivate and maintain Bacillus at a high concentration because the activity of general bacteria is predominant and lacks the nutrients required for Bacillus. As a way to improve this, by varying the operating conditions, such as sensitizers, to try to dominate Bacillus bacteria, this takes a considerable period of time and difficult to maintain the growth and high concentration of Bacillus bacteria.

The present invention is to solve such a conventional problem, the purpose of the new type of sewage, which can improve the treatment capacity by effectively and stably cultivating microorganisms used in biological treatment of sewage, manure and industrial wastewater, To provide a microbial culture agent for use in the biological treatment of manure and industrial wastewater and a method for producing the same. In particular, the present invention promotes the growth and proliferation of spore formation and germination of the Bacillus microorganism group, which is excellent in the treatment of organic wastewater and also excellent in the removal of nitrogen and phosphorus, promotes its growth and proliferation, and significantly enhances the activity of Bacillus bacteria. It is an object of the present invention to provide a microbial culture agent and its preparation method for use in biological treatment of sewage, manure and industrial wastewater in a new form that can be predominantly cultured.

1 is a diagram showing microbial cultures for use in the biological treatment of sewage, manure and industrial wastewater in accordance with an embodiment of the present invention;

Figure 2 is a diagram showing the state of the treated water when the culture medium 1 applied to the J sewage treatment plant of the microbial culture of Figure 1;

Figure 3 is a photograph showing the culture state of Bacillus bacteria in the reactor of the J sewage treatment plant obtained the result of Figure 2;

Figure 4 is a diagram showing the state of the treated water when the culture medium 2 of the microbial culture of Figure 1 applied to the Y manure treatment plant;

Figure 5 is a photograph showing the culture of Bacillus bacteria in the reaction tank of the Y manure treatment plant obtained the result of Figure 4;

Figure 6 is a diagram showing the state of the treated water when the culture medium 3 of the microbial cultures of Figure 1 applied to the D food wastewater treatment plant;

Figure 7 is a photograph showing the culture of Bacillus bacteria in the reaction tank of the D food wastewater treatment plant obtained in the result of FIG.

According to a feature of the present invention for achieving the above object, the microbial culture agent for use in the biological treatment of sewage, manure and livestock, and industrial wastewater of the present invention includes siliceous fertilizer and magnesium sulfate heptahydrate.

The siliceous fertilizer contains silicon, manganese and magnesium. This siliceous fertilizer is mainly used for the leaching of silicon, and the leaching amount of manganese and magnesium is very low. At this time, the silicon is a component necessary for the growth of vegetative cells of Bacillus bacteria to filamentous body. The magnesium sulfate heptahydrate contains magnesium and sulfur components. Magnesium activates and stabilizes various macromolecules in microbial cells. Sulfur contains amino acids and various vitamins necessary for microorganisms.

Such a microbial culture agent of the present invention promotes the growth of microorganisms while supplying mineral components. In particular, the microbial culture agent of the present invention allows Bacillus bacteria to dominate culture. The microbial culture agent may also be referred to as a microbial activator, microbial nutrient, microbial growth agent and microbial growth promoter.

Such a microbial culture agent of the present invention may further include peat in its preferred embodiment. In addition, blast furnace slag, diatomaceous earth, slaked lime, zinc sulfate and copper sulfate may be further included. In addition, blast furnace slag, diatomaceous earth, peat, slaked lime, zinc sulfate, copper sulfate and manganese chloride tetrahydrate may be further included.

The peat contains a large amount of Bacillus bacteria, and elutes various mineral components necessary for the growth of Bacillus bacteria. The blast furnace slag contains silicon, manganese, iron and the like, mainly for the purpose of elution of iron, to improve the sedimentation of the sludge. At this time, iron is a cofactor of the respiratory enzyme of Bacillus. The slaked lime is a component for replenishing calcium. At this time, calcium serves to increase the durability when Bacillus bacteria form spores. The zinc sulfate, copper sulfate and manganese chloride tetrahydrate are added to replenish zinc, copper and manganese, respectively. At this time, zinc and copper act as cofactors of various enzyme actions, and manganese is a necessary component when inducing sporulation of Bacillus bacteria.

As described above, the microbial culture agent of the present invention is 55-65% of the siliceous fertilizer, 18-22% of the peat, and the magnesium sulfate heptahydrate, based on 100% of the total mixture of the siliceous fertilizer, peat, and magnesium sulfate heptahydrate. It may consist of a mixing ratio of 18-22%. Also, with respect to 100% of the mixture consisting of siliceous fertilizer, blast furnace slag, diatomaceous earth, magnesium sulfate heptahydrate, slaked lime, zinc sulfate and copper sulfate, 43-47% siliceous fertilizer, 28-32% blast furnace slag, and 7- diatomaceous earth 9%, magnesium sulfate heptahydrate may be 11-13%, slaked lime 3-5%, zinc sulfate 0.3-0.7% and copper sulfate 0.3-0.7%. In addition, for the total amount of the mixture consisting of siliceous fertilizer, blast furnace slag, diatomaceous earth, peat, magnesium sulfate heptahydrate, slaked lime, zinc sulfate, copper sulfate and manganese chloride tetrahydrate, the siliceous fertilizer is 19-23%, and the blast furnace slag is 38-. 42%, diatomaceous earth 7-9%, peat 11-14%, magnesium sulfate heptahydrate 11-13%, slaked lime 3-5%, zinc sulfate 0.3-0.7%, copper sulfate 0.3-0.7%, chloride Manganese tetrahydrate may consist of a mixing ratio of 1.5-2.5%.

In order to facilitate the growth of Bacillus bacteria, the components and the mixing ratio of such a culture agent were selected through the experiment to select the dissolution concentration of each component, and then the elution concentration of the necessary components was determined through the dissolution test of the raw materials containing each component. Next, it is calculated and calculated so that more than the target concentration can be eluted based on the dissolution concentration. Therefore, when it is out of the range of the mixing ratio of each component constituting the culture medium as described above, the effective function as a culture medium for dominant Bacillus bacteria is not exhibited.

According to another feature of the present invention for achieving the object as described above, the method for producing a microbial culture agent for use in the biological treatment of sewage, manure and livestock, and industrial wastewater is to grind so that the particle size of peat is 200 mesh or more Step and; And putting the siliceous fertilizer and magnesium sulfate heptahydrate into the pulverized peat in a mixer and mixing for a predetermined time.

According to another feature of the present invention for achieving the above object, the method for producing a microbial culture agent for use in the biological treatment of sewage, manure and livestock, and industrial wastewater so that the particle size of the blast furnace slag is 200 mesh or more Grinding; The pulverized blast furnace slag includes a step of mixing the silicate fertilizer, diatomaceous earth, magnesium sulfate heptahydrate, slaked lime, zinc sulfate and copper sulfate in a mixer for a predetermined time.

According to another feature of the present invention for achieving the above object, the method for producing a microbial culture agent for the biological treatment of sewage, manure and livestock, and industrial wastewater is 200 mesh particle size of blast furnace slag and peat Grinding to be abnormal; The pulverized blast furnace slag and peat include a step of mixing the silicate fertilizer, diatomaceous earth, magnesium sulfate heptahydrate, slaked lime, zinc sulfate, copper sulfate and manganese chloride tetrahydrate in a mixer for a predetermined time.

On the other hand, in the above methods, the reason for limiting the particle size of peat and blast furnace slag to 200 mesh or more is that if the particle size of peat and blast furnace slag is large, the elution of the necessary components is lowered, and a larger amount must be added, and the water is dissolved in This is to prevent the metering from being difficult due to clogging of the pipe due to accumulation in the lower part of the dissolution tank and the pipe during the injection. If the particle size of peat and blast furnace slag particles is 200 mesh or more, the particles are dispersed and the amount of elution of necessary components is increased and dissolution and injection are easy.

Such a method of preparing a microbial culture agent for use in biological treatment of sewage, manure and livestock, and industrial wastewater of the present invention, in the preferred embodiment, the mixing time may be 7 minutes to 13 minutes.

In the present invention, the Bacillus microorganism group, which is a microorganism to be cultured, is a gram-positive coliform bacterium that is morphologically belonging to a simple bacilli and can maintain an endogenous spore in an adverse environment. The dominant culture and high concentration growth of Bacillus bacteria utilize the means of filamentous cell formation avoided by causing sludge swelling in treatment plants such as sewage, manure and industrial wastewater (hereinafter referred to as wastewater). In other words, Bacillus bacteria grows rapidly in a state of sufficient nutrients, and the filamentous bodies are connected to each other without falling off, and under such rapid growth, most of the nutrients are consumed to reach a nutrient deficient state. At this time, if the increase in the cell mass through the increase in the return cost, the individual cells forming the filamentous body becomes a severe lack of nutrients to reach the limit. Then, as the spores are formed, the secretion of various antibiotics and the mucous substances are discharged to promote sedimentation and the filamentous bodies are disassembled to increase the number of cells including spores. Spores thus formed germinate under the supply of nutrients and grow into new cells, so exponential growth of cells is possible.

Therefore, the microbial culture agent of the present invention is composed of components capable of promoting the growth of Bacillus bacteria while supplying various mineral components necessary for the growth and sporulation of Bacillus bacteria and spores germination again. Bacillus bacteria that are promoted by the use of the microbial culture agent of the present invention include Bacillus subtilis, Bacillus licheniformis, Bacillus fastidiosus, Bacillus sphaericus, Bacillus megaterium, Bacillus polymyxa, Bacillus macerans and the like.

On the other hand, in the preparation of the microbial culture medium according to the present invention, each of the components should be mixed according to the mixing ratio within a predetermined range, if spore formation and germination of Bacillus bacteria when the mixing ratio of each component is out of the predetermined range This affects the performance of the culture agent.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to FIGS. 1 to 7.

1 is a diagram illustrating microbial cultures for use in the biological treatment of sewage, manure and industrial wastewater in accordance with an embodiment of the present invention.

Referring to Figure 1, the microbial culture for Bacillus according to an embodiment of the present invention is made of three kinds in particular to be used according to the type of waste water. For convenience of explanation, three kinds of microbial culture agents will be described as culture agent 1, culture agent 2, and culture agent 3 below. Culture agent 1 is intended for use in a treatment plant for treating sewage, culture agent 2 is specifically used for a treatment plant for treating manure and livestock waste water, and culture agent 3 is specifically used for a plant treating industrial waste water, ie, food waste water. It is to use.

The culture agent 1 is a microbial culture agent composed mainly of siliceous fertilizer, peat, and magnesium sulfate heptahydrate (salt salt; MgSO ₄ · 7H ₂ O). The culture agent 1 may be composed of 55-65% of the siliceous fertilizer, 18-22% of the peat, and 18-22% of the magnesium sulfate heptahydrate based on 100% of the total amount of the mixture. In the present preferred embodiment, the culture agent 1 comprises 60% of the siliceous fertilizer, 20% of the peat, and 20% of the magnesium sulfate heptahydrate. In the production method of the culture agent 1, first, the peat is pulverized so that the particle size is 200 mesh or more. Peat, siliceous fertilizer and magnesium sulfate heptahydrate pulverized to a particle size of 200 mesh or more are added to the mixer at the above-described mixing ratio and mixed for about 10 minutes. In a preferred embodiment, the ground peat is 20% based on the total amount of the mixture, the siliceous fertilizer is 60% and the magnesium sulfate heptahydrate is 20%. Culture medium 1 made in this way is packaged in a packaging machine to be used externally.

Culture medium 2 is a microorganism culture consisting of siliceous fertilizer, blast furnace slag, diatomite, magnesium sulfate heptahydrate, slaked lime (Ca (OH) ₂ ), zinc sulfate and copper sulfate (CuSO ₄ ) as main ingredients. Jay. Culture medium 2 is 43-47% siliceous fertilizer, 28-32% blast furnace slag, 7-9% diatomaceous earth, 11-13% magnesium sulfate hydrate, 3-5%, the zinc sulfate is 0.3-0.7% and the copper sulfate may be composed of a mixing ratio of 0.3-0.7%. In the present preferred embodiment, the culture agent 2 is 45% of the siliceous fertilizer, the blast furnace slag is 30%, the diatomaceous earth is 8%, the magnesium sulfate heptahydrate is 12%, the calcined lime is 4%, the zinc sulfate is 0.5% % And the copper sulfate consists of 0.5%. In the production method of the culture agent 2, first, the blast furnace slag is pulverized so that the particle size is 200 mesh or more. Blast furnace slag pulverized to a particle size of 200 mesh or more, siliceous fertilizer, diatomaceous earth, magnesium sulfate heptahydrate and slaked lime were added to the mixer at the above-mentioned mixing ratio and mixed for about 10 minutes. Put into the mixer and mix for about 10 minutes. In a preferred embodiment, the ground blast furnace slag is 30% based on the total amount of the mixture, the siliceous fertilizer is 45%, the diatomaceous earth is 8%, the magnesium sulfate heptahydrate is 12%, the calcined lime is 4%, the zinc sulfate is 0.5 % And the copper sulfate is made to 0.5%. In this way, the culture agent 2 is packaged in a packaging machine to be used externally.

Culture medium 3 is a microbial culture medium consisting of siliceous fertilizer, blast furnace slag, diatomaceous earth, peat, magnesium sulfate heptahydrate, slaked lime, zinc sulfate, copper sulfate and manganese chloride tetrahydrate. In the culture medium 3, the siliceous fertilizer was 19-23%, the blast furnace slag was 38-42%, the diatomaceous earth was 7-9%, the peat was 11-14%, and the magnesium sulfate heptahydrate was 100% of the total amount of the mixture. 11-13%, the lime is 3-5%, the zinc sulfate 0.3-0.7%, the copper sulfate 0.3-0.7% and the manganese chloride tetrahydrate may be composed of a mixing ratio of 1.5-2.5%. In a preferred embodiment of the culture agent 3, the siliceous fertilizer is 21%, the blast furnace slag is 40%, the diatomaceous earth is 8%, the peat is 12%, the magnesium sulfate heptahydrate is 12%, the lime is 4% The zinc sulfate is 0.5%, the copper sulfate is 0.5% and the manganese chloride tetrahydrate is 2%. In the production method of the culture agent 3, first, the blast furnace slag and peat are pulverized to have a particle size of 200 mesh or more. Blast furnace slag, peat, siliceous fertilizer, diatomaceous earth, magnesium sulfate heptahydrate and slaked crushed to a particle size of 200 mesh or more were put in a mixer at the above-mentioned mixing ratio and mixed for about 10 minutes, and zinc sulfate, copper sulfate and chloride were added to the mixed mixture. Manganese tetrahydrate is added to the mixer at the above-mentioned mixing ratio and mixed for about 10 minutes. In a preferred embodiment, the ground blast furnace slag is 40% based on the total amount of the mixture, the peat is 12%, the siliceous fertilizer is 21%, the diatomaceous earth is 8%, the magnesium sulfate heptahydrate is 12%, the slaked lime is 4% The zinc sulfate is 0.5%, the copper sulfate is 0.5% and the manganese chloride tetrahydrate 2%. Culture medium 3 made in this way is packaged in a packaging machine to be used externally.

Figure 2 is a diagram showing the state of the treated water when the culture medium 1 of the microbial culture of Figure 1 applied to the J sewage treatment plant, Figure 3 is a culture state of Bacillus in the reaction tank of the J sewage treatment plant obtained the result of FIG. Is a picture showing.

Figure 2 shows the results of obtaining a highly treated water while normalizing the treatment plant by administering a culture agent 1 for Bacillus bacterium according to a preferred embodiment of the present invention to the J sewage treatment plant. The J sewage treatment plant was designed and operated to treat 16,000 ㎥ of sewage using Bacillus bacteria per day. However, the results of the operation for the first two months before the experiment using the culture agent 1 did not reach a constant level of the water quality of the treated water, and the total number of bacteria and the number of Bacillus bacteria in the reactor were kept significantly low. As a result of administering 2% of the culture agent 1 to the biochemical oxygen demand (BOD) to the J sewage treatment plant, the treated state of the sewage was improved, and the BOD before the use of the culture agent 1 was 20.8. Indicated. In addition, as shown in FIG. 3, the results of the experiment using the culture agent 1 showed that the total number of bacteria and the number of Bacillus bacteria increased rapidly, and the number of Bacillus bacteria was more than 90% of the total number of bacteria. .

As shown in FIG. 3 (the same is true for FIGS. 5 and 7 to be described later), Bacillus bacteria isolated from the sludge of each treatment plant were synthesized by synthesizing artificial media containing each component (sewage, manure and industrial wastewater) in a laboratory. Inoculation is a culture experiment. The rate of microbial culture was 1% of BOD of artificial media, maintained at 35 ℃ and pH 6.5-7.5, and the microbial state was taken after aerobic cultivation. The image captured by the microscope was outputted using an in-lab imaging system equipped with a back light.

Figure 4 is a diagram showing the state of the treated water when the culture medium 2 of the microbial culture of Figure 1 applied to the Y manure treatment plant, Figure 5 is a culture state of Bacillus in the reaction tank of the Y manure treatment plant obtained the result of FIG. Is a picture showing.

Figure 4 shows the result of obtaining a highly treated water while normalizing the treatment plant by administering the culture agent 2 for Bacillus bacteria of the preferred embodiment of the present invention to the Y manure treatment plant. Y manure treatment plant was designed and operated to treat 270㎥ of bacteria per day using Bacillus bacteria. However, the experiment was maintained for at least two months for the adaptation and activation before the experiment using the culture agent 2, the water quality of the treated water did not reach a certain level, the ammonia odor and odor in the reaction tank was not treated I was driving. As a result of administering 2% of the culture agent 2 compared to BOD in the Y manure treatment plant, after 10 days, the ammonia odor and odor disappeared and the total nitrogen (T-N) was remarkably decreased. In addition, as a result of measuring the total number of cells, the difference was significantly different from that before the administration of the culture agent 2, and the ratio of other various bacteria was greatly reduced. In addition, as shown in Figure 5, the total cell concentration of Bacillus bacteria increased more than 92%, the number of Escherichia coli rapidly decreased to maintain 10 / ml or less.

Figure 6 is a diagram showing the state of the treated water when the culture medium 3 of the microbial cultures of Figure 1 applied to the D food wastewater treatment plant, Figure 7 is a bacterium of Bacillus in the reaction tank of the D food wastewater treatment plant obtained the result of FIG. Photo shows the culture.

Referring to Figure 6, by administering the culture agent 3 for Bacillus bacteria according to a preferred embodiment of the present invention to the D food wastewater treatment plant of the starch manufacturing plant shows a result of obtaining a highly treated water while normalizing the treatment plant. The D food wastewater treatment plant was designed and operated to treat 2,700 m 3 of food wastewater per day using Bacillus bacteria. However, the results of operation for the first two months before the experiment using the culture medium 3, the water quality did not reach a certain level, especially the chemical oxygen demand (COD) was high, the total number of cells in the reactor and The number of Bacillus bacteria was kept significantly low. As a result of administering 2% of the culture agent 3 to BOD in the food waste water treatment plant as described above, the treatment state of the food waste water was improved. As shown in FIG. 7, the total number of bacteria and the number of Bacillus bacteria increased rapidly. The number of Bacillus bacteria accounted for more than 90% of the total number of bacteria.

In a treatment plant where contaminants are removed by mixing and culturing a large number of microorganisms in general wastewater, Bacillus bacteria grow rapidly and form filaments when an environment suitable for growth is established. However, in an adverse environment, spores form and enter a state of rest and do not grow any more, and germinate and multiply when an environment of suitable conditions is formed again. Therefore, the microbial culture agent according to the above-described microbial culture agent and production method is administered to the wastewater treatment plant using Bacillus bacteria, so that Bacillus bacteria are dominantly cultured at a high concentration.

In a preferred embodiment of the present invention, the microbial culture agent for Bacillus bacteria, in particular three kinds of microbial culture agent to be used in accordance with the characteristics of the object to be treated in order to efficiently remove sewage, manure and industrial wastewater (food wastewater) to provide. This is because Bacillus microorganisms are different in the type of Bacillus bacteria predominant depending on the type of substrate (wastewater). In addition, the types of Bacillus bacteria predominant in the treatment of sewage, organic wastewater and livestock manure are 8-12 species among the total of about 40 Bacillus genus. This is because the mineral content is different. For example, in the case of sewage, calcium and various inorganic salts were sufficiently included in the amount of BOD, and thus, they were not added separately, and the necessary mineral components were supplemented by adding peat, which contains a large amount of Bacillus bacteria, which appears a lot during sewage treatment.

According to the present invention, it is possible to remarkably promote the growth of Bacillus bacteria, a group of microorganisms excellent in the treatment of organic wastewater and also excellent in the removal of nitrogen and phosphorus, showing excellent effects on spore formation and germination, etc. Dominate and maintain high concentrations quickly. Thus, the biological treatment of sewage, manure and industrial wastewater can be carried out effectively and reliably.

Claims (11)

A microbial culture agent for use in the biological treatment of sewage, manure, and industrial wastewater, which promotes the growth of microorganisms by supplying minerals, including siliceous fertilizers and magnesium sulfate heptahydrate. The method of claim 1, Microbial culture for use in the biological treatment of sewage, manure and industrial wastewater further comprising peat. The method of claim 1, A microbial culture agent for use in the biological treatment of sewage, manure and industrial wastewater further comprising blast furnace slag, diatomaceous earth, slaked lime, zinc sulfate and copper sulfate. The method of claim 1, A microbial culture for use in the biological treatment of sewage, manure and industrial wastewater further comprising blast furnace slag, diatomaceous earth, peat, slaked lime, zinc sulfate, copper sulfate and manganese chloride tetrahydrate. The method of claim 2, The sewage consisting of 55-65% of the siliceous fertilizer, 18-22% of the peat and 18-22% of the magnesium sulfate heptahydrate with respect to 100% of the total mixture of the siliceous fertilizer, peat and magnesium sulfate heptahydrate. Microbial media for use in biological treatment of waste, manure and industrial wastewater. The method of claim 3, wherein The total amount of the mixture consisting of siliceous fertilizer, blast furnace slag, diatomaceous earth, magnesium sulfate heptahydrate, slaked lime, zinc sulfate and copper sulfate is 43-47% siliceous fertilizer, 28-32% blast furnace slag and 7-9% diatomaceous earth. , Microorganisms for use in the biological treatment of sewage, manure and industrial wastewater with a mixture of 11-13% magnesium sulfate heptahydrate, 3-5% slaked lime, 0.3-0.7% zinc sulfate and 0.3-0.7% copper sulfate Culture agent. The method of claim 4, wherein For the total amount of the mixture consisting of siliceous fertilizer, blast furnace slag, diatomaceous earth, peat, magnesium sulfate heptahydrate, slaked lime, zinc sulfate, copper sulfate and manganese chloride tetrahydrate, the siliceous fertilizer is 19-23%, the blast furnace slag is 38-42% , 7-9% diatomaceous earth, 11-14% peat, 11-13% magnesium sulfate heptahydrate, 3-5% slaked lime, 0.3-0.7% zinc sulfate, 0.3-0.7% copper sulfate, manganese chloride Cargo is a microbial culture for use in the biological treatment of sewage, manure and industrial waste at a mixing ratio of 1.5-2.5%. Grinding the peat to have a particle size of at least 200 mesh; Method of producing a microbial culture agent for use in the biological treatment of sewage, manure and industrial wastewater comprising the step of mixing the silicate fertilizer and magnesium sulfate heptahydrate in the pulverized peat in a mixer for a predetermined time. Grinding the blast furnace slag to a particle size of at least 200 mesh; Microbial cultivation for use in the biological treatment of sewage, manure and industrial wastewater comprising the step of mixing the pulverized blast furnace slag with siliceous fertilizer, diatomaceous earth, magnesium sulfate heptahydrate, slaked lime, zinc sulfate and copper sulfate in a mixer for a certain period of time. Preparation method of the agent. Grinding the blast furnace slag and peat so as to have a particle size of 200 mesh or more; Biological fertilization of sewage, manure and industrial wastewater comprising the step of mixing the pulverized blast furnace slag and peat into a mixer for a period of time by adding siliceous fertilizer, diatomaceous earth, magnesium sulfate heptahydrate, hydrated lime, zinc sulfate, copper sulfate and manganese chloride tetrahydrate to a mixer. A method of preparing a microbial culture agent for use in processing. The method according to any one of claims 8 to 10, The method of producing a microbial culture for use in the biological treatment of sewage, manure and industrial wastewater, wherein the mixing time is 7 minutes to 13 minutes.