KR20010017053A - Cosmetic composition containing extracts of Calyx kaki, Lasiophaera Seu Calvatin or Fructus polygoni Hydropiper - Google Patents

Abstract

PURPOSE: A cosmetic composition containing Calyx kaki extract, Lasiophaera Seu Calvatin extract, or Fructus polygoni Hydropiper extract as an effective ingredient is provided, which improves liver spots and freckles, and shows good skin whitening effect without side effect. CONSTITUTION: A process for the preparation of Calyx kaki extract, Lasiophaera Seu Calvatin extract, or Fructus polygoni Hydropiper extract comprises: pulverizing each Calyx kaki, Lasiophaera Seu Calvatin, or Fructus polygoni Hydropiper, and extracting by boiling 3hrs in a circulation extractor after adding water, anhydrous or hydrated primary alcohol of carbon number 1-4, ethylacetate or acetone; filtering with a 100mesh filter sheet, and extracting the residue one more time by same process; adding each extract, and filtering with a Whatman No.2 filter sheet at room temperature to eliminate non-soluble materials; concentrating under decompression at 60deg.C using a distiller with a cooling condenser, and freeze-drying to get each extract powder. The cosmetic composition contains 0.0001-10.0wt.% of Calyx kaki extract, Lasiophaera Seu Calvatin extract, or Fructus polygoni Hydropiper extract.

Description

Cosmetic composition containing corpse extract, quince extract or raal extract extract {Cosmetic composition containing extracts of Calyx kaki, Lasiophaera Seu Calvatin or Fructus polygoni Hydropiper}

The present invention relates to a cosmetic composition containing a carcass extract, a hair extract or a raal extract. More specifically, the present invention relates to a cosmetic composition containing at least one selected from carcass extracts, horsetail extracts and raal extracts with excellent melanin production inhibitory effect as an active ingredient.

It is everyone's constant desire to have white, fair skin. Human skin color is genetically determined by the concentration and distribution of melanin in the skin, but is also influenced by environmental or physiological conditions such as solar ultraviolet rays, fatigue and stress. Melanin is a type of amino acid tyrosine (tyrosine) enzymes called tyrosinase (tyrosinase) acts to convert to dopa (DOPA), dopaquinone (dopaquinone) is produced through a non-enzymatic oxidation reaction. However, although the pathway through which melanin is made is known, it is still unknown in detail the mechanism by which tyrosinase acts, the mechanism that induces melanin synthesis.

On the other hand, by inhibiting the synthesis of melanin pigment can not only realize the skin whitening by brightening the skin tone, but also improve the hyperpigmentation of skin such as blemishes, freckles due to ultraviolet rays, hormones, heredity.

Therefore, conventionally, substances having inhibitory activity against tyrosinase, such as hydroquinone, ascorbic acid, kojic acid, and glutathione, are incorporated into ointments or cosmetics, and thus whitening, blemishes, and freckles of skin. Although it has been used for the purpose of improvement, the whitening effect of hydroquinone is recognized, but the amount of use is extremely limited due to severe skin irritation. In addition, ascorbic acid is easily oxidized, and cosmetics containing the same cause problems such as discoloration and deodorization, and thiol compounds such as glutathione and cysteine have a characteristic unpleasant odor and have problems with transdermal absorption. In addition, these glycosides and derivatives have a high polarity, so there is a problem to use them as a component of a cosmetic. In addition, placenta extract has a problem that the whitening effect is insufficient.

Therefore, the present inventors have developed numerous whitening agents to develop new whitening agents in native plants and animals using B16 mouse melanoma cells, which can screen even substances that inhibit melanin synthesis induction. As a result of repeated experiments, it was found that the carcass extract, the hair extract extract, and the ralli extract have a very strong melanin production inhibitory effect and a whitening effect, and completed the present invention.

The present invention relates to a cosmetic composition exhibiting blemishes, freckles, and skin whitening effects, characterized in that it contains at least one selected from carcass extracts, quince extracts and raal extracts as an active ingredient.

The carcass is a calyx of the genus Disopyros kaki L, a deciduous tree of the Ebenaceae family. The Latin name is Calyx kaki. The body has been used as a herbal medicine such as vomiting and coughing. Carcass components are known to include substances such as succinic acid, quercetin, kaempferol, neodiospyrin, betulin, and daucosterol.

Laval is the dried fruit of Lasioshpaera fenzlii Reich. Of the Lycopodiaceae family. The Latin name is Lasiophaera Seu Calvatin. The pill has been used in oriental medicine for hemostatic and antibacterial effects such as trauma bleeding and nosebleed (Chinese medicine dictionary). The constituents of the hair are known to include substances such as leucine, tyrosine, uream, ergosterol, and gemmatein.

Laurea is the fruit of Polygonum hydropiper L. (Glyphaceae), a plant of the Polygonaceae family. Latin name is Fructus polygoni Hydropiper. Lalary has been used as a herbal medicine to treat diuresis, tosaboktok, edema, etc. (Chinese medicine dictionary). The constituents of rallies are known to include substances such as gallic acid, alanine, serine, arginine, quercetin, rutin, hyperoxide, fuginin and drimeol. .

According to the present invention, the carcass, marbal, and raal, which can be purchased as a medicine, are first extracted with an organic solvent such as anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms or ethyl acetate or acetone, and then concentrated under reduced pressure, and then lyophilized or spray dried. Prepare the primary extract. The carcass, marbal, and ralyo extracts prepared in this way have very potent melanin synthesis inhibitory effects in B-16 mouse melanoma cells. In addition, when the external body ointment or cosmetics are prepared with dried carcass, horsetail, and racial extract, and applied to human skin, the skin whitening effect is very excellent.

In addition, commercially available carcasses, yams, and rallies are available for pulverization and are refluxed with 5-20 volumes of water or 1-4 anhydrous or lower alcohols, ethyl acetate or acetone with respect to the dry weight of the crushed powder. Extract by heating 1-5 hours at 50-100 ° C. in the extractor. After filtering with filter cloth, the residue is extracted one more time in the same manner. The extracts were combined and concentrated under reduced pressure, followed by freeze drying or spray drying to obtain a dry extract. When dried carcass, horsetail, and raloretus extracts were treated with B-16 mouse melanoma cells, they showed very potent melanin synthesis inhibitory effects.

By using the obtained body, hair, raal extracts as described above to prepare a variety of cosmetics, such as skin external ointment or lotion, cream, supple cosmetics, nourishing cosmetics, packs, essences. It was found that the skin whitening effect was very excellent when the cosmetics containing the body, the hair, and the rala extract were prepared and applied to the skin of a person. In addition, no side effects occur, indicating that carcass, hydration and raal extracts are safe and highly effective for improving blemishes and freckles and skin whitening agents.

In the preparation of external skin ointments or cosmetics, carcass extracts, horse extracts, raal extracts, etc. are appropriately 0.0001-10.0% by weight, preferably 0.0005-10.0% by weight in dry weight. If the concentration of the extract is less than 0.0001% by weight can not expect a distinct effect, at a concentration of more than 10% by weight does not show any increase in effect as the content increases.

Hereinafter, although this invention is demonstrated in detail based on an Example, it turns out that the technical scope of this invention is not limited only to these Examples.

Example 1-18

100 g of a pulverized crushed product obtained from a commercially available carcass, a mash and a ralcum was put in 3 L of a solvent such as water, anhydrous or hydrous lower alcohol having 1 to 4 carbon atoms, ethyl acetate or acetone and 3 hours in a reflux extractor with a cooling capacitor. After boiling and extracting, the resultant was filtered with 100 mesh filter cloth and the residue was extracted once more in the same manner. Each extract was combined and filtered at room temperature with Whatman No. 2 filter paper to remove insoluble materials, and then concentrated under reduced pressure at 60 ° C. in a distillation apparatus equipped with a cooling condenser and freeze-dried to dry each dry weight as shown in Table 1 below. Got.

Experimental Example 1

Each of the extracts obtained according to Examples 1-18 was added to the culture medium of B-16 mouse melanoma cells to test the whitening effect at the cellular level (see Lotan R., Lotan D. Cancer). Res. 40: 3345-3350, 1980). Each extract obtained according to Example 1-18 was added to a culture medium of B-16 melanoma cells with a hydroquinone aqueous solution so as to have a final concentration of 0.005%, followed by incubation for 3 days, followed by trypsin treatment. Melanin was extracted from the culture vessel by centrifugation. Sodium hydroxide solution thereto (1N concentration) was added to dissolve the melanin 1㎖ boiled 10 minutes using a spectrophotometer at 400 nanometers (㎚) the amount of the unit cell allowance (10 ⁶ cell) of melanogenesis by measuring the absorbance at It was carried out by the method shown in the absorbance, the relative melanin production relative to the control was calculated as the inhibition rate (%). The results are shown in Table 2.

As can be seen from the results of Table 2, considering that each sample obtained according to Examples 1-18 is an extract that is not purified at all, compared to hydroquinone, a known whitening substance, it is very effective for the melanoma cells of cultured mice. It can be seen that there is a strong melanin production inhibitory ability.

Experimental Example 2.

Melanoma cells of rats cultured in the same manner as in Experimental Example 1 were examined for their ability to inhibit melanin production according to the concentration of the extracts of Examples 1, 7 and 13. The results are shown in Table 3 below.

As can be seen in the results of Table 3, it can be seen that the corpse extract, the valse extract, and the ralyo extract have a very strong melanin production inhibitory ability against the cultured melanoma cells. Hydroquinone has a strong melanin production inhibitory ability at low concentrations, but the cytotoxicity is severe and can not be tested at 0.0001% or more, whereas the extract of the present invention is the maximum amount of hydro that can be used without showing cytotoxicity even at a concentration of 0.05% or more It has a higher melanin production inhibitory effect than quinone. In addition, since the body extract, hair extract, and raal extract have a very high melanin production inhibitory ability, considering that it is not a single substance but a mixture, it can be very useful for improving freckles and freckles or skin whitening.

Hereinafter, the preparation example of the cosmetics containing a corpse, a paw, and a racial extract is shown by the manufacture example.

Preparation Example 1-3 and Comparative Example 1. Skin Ointment

According to the prescription shown in Table 4 below, a skin external ointment containing a body, a paw, and a ladle extract was prepared. Here, carcass, horsetail, and ralyo extract refers to those of Examples 5, 11, and 17, respectively.

Preparation Example 4-6 and Comparative Example 2. Cream cosmetics

According to the formulation shown in Table 5 below, the cream in the cosmetics containing the carcass, the paw, the rale extract extract. Herein, carcass, horseradish and ralyo extracts refer to those of Examples 5, 13 and 21, respectively.

Preparation Example 7-9 and Comparative Example 3. Flexible Cosmetics

According to the formulation shown in Table 6 below, a flexible cosmetic water in the cosmetics containing the carcass, scabbard, raalic extract was prepared. Herein, carcass, horseradish and ralyo extracts refer to those of Examples 5, 13 and 21, respectively.

Preparation Example 10-12 and Comparative Example 4. Nutrients

According to the prescription shown in Table 7 was prepared nutrient cosmetics in the cosmetics containing the carcass, horseshoe, raal extract. Herein, carcass, horseradish and ralyo extracts refer to those of Examples 5, 13 and 21, respectively.

Preparation Example 13-15 and Comparative Example 5. Pack

According to the formulation shown in Table 8 below, a pack of cosmetics containing carcass, horsetail, and raal extract was prepared. Herein, carcass, horseradish and ralyo extracts refer to those of Examples 5, 13 and 21, respectively.

Preparation Example 16-18 and Comparative Example 6. Essence

According to the prescription shown in Table 9 below, the essence of the cosmetics containing the carcass, the hair, the raal extract extracts. Herein, carcass, horseradish and ralyo extracts refer to those of Examples 5, 13 and 21, respectively.

Experimental Example 3

Twenty healthy men and women were selected to attach aluminum foil in two rows of six holes with a diameter of 7 mm in the lower part of both arms and irradiated with 60mJ / ㎠ light using a ORIEL solar simulator 1000W at a distance of 10 cm from the arm. It was. The irradiation site was washed well with 70% ethanol aqueous solution before irradiation. Twice a day from 3 days before the irradiation to 3 weeks after the irradiation, the samples prepared according to Preparation Examples 1-18 and Comparative Examples 1-6 and the samples containing the extracts of carcasses, paws, and ladles were prepared. It was applied in the same row in pairs (for pack formulations of Preparation Examples 13, 14, 15 and Comparative Example 5, it was removed 15 minutes after application). For each, the pigmentation degree of the preparation example and the comparative example was visually judged, and the degree to which the preparation example suppressed the pigmentation compared to the comparative example was evaluated in three stages of distinct effects, effects, and no differences, and the results are shown in Table 10 Same as

As can be seen from the results in Table 10 above, the whitening ointment and cosmetics containing the carcass, bristles and raal extracts prepared according to Preparation Examples 1-18 showed a whitening effect on at least 9 of 20 subjects. In the comparative experiments of Preparation Examples 1, 2, 3 and Comparative Example 1, in which a large amount of designated, Wasong extract was applied, 73% showed pigmentation inhibitory effect, and no side effects occurred in the skin. It is a safe and effective cosmetics for improving blemishes and freckles or skin whitening.

According to the present invention, the cosmetic composition containing at least one selected from carcass extracts, horse extracts and raal extracts as an active ingredient has excellent blemishes, freckles, and skin whitening effects, but does not exhibit any side effects in the skin.

Claims (3)

Cosmetic composition showing a blemish, freckles improvement and skin whitening effect, characterized in that it contains one or more selected from a body extract, a claw extract and a rale extract as an active ingredient. The cosmetic composition according to claim 1, which contains 0.0001-10.0% by weight of a carcass, a paw, and a raal extract in dry weight. The extract according to claim 1, which is obtained by extracting the carcass, the bristles and the rallies with water or a lower alcohol having 1 to 4 carbon atoms, ethyl acetate or acetone at least once, and distilling the extract under reduced pressure followed by freeze drying or spray drying. Cosmetic composition characterized in that.