KR20000060970A - Black Tea Which Contains Nephrite Jade Powder And Process For Preparing Thereof - Google Patents

Abstract

PURPOSE: Method for preparing black tea containing Nephrite powder that it can have medical effects by mixing Nephrite powder with black tea prepared through existing ways is provided. CONSTITUTION: The black tea containing Nephrite powder is provided by mixing 3-10wt% Nephrite powder of 150-1000 granularity with a usual mixer while manufacturing black tea, and then packing in a small quantity for comfortable drinking. And the black tea containing Nephrite powder can be prepared by heating into concentrated liquid of a certain degree brix, and then mixing 3-10 wt% of Nephrite. The composition of the Nephrite powder comprises silicon 34%, magnesium 10%, calcium 4.9%, iron 0.23%, aluminum 0.16%, cooper 0.17%, cobalt 0.046%, manganese 0.14%, tin 0.024%, beryllium 0.00072%, silver 0.0013%, titanium 0.0038%, nickel 0.0028%, and chromium 0.0030%. Thereby, the black tea prepared through this method has a synergy of the germination and detoxification effects by black tea and the functions of Nephrite jade activating cells, removing heavy metals, regulating pH, and softening hard water, which are verified by various experiments.

Description

Black Tea Which Contains Nephrite Jade Powder And Process For Preparing Them {Black Tea Which Contains Nephrite Jade Powder And Process For Preparing Thereof}

The present invention relates to black tea containing nephrite powder and a method for manufacturing the same, in particular, the ability to cure diseases of the human body (headache, insomnia, indigestion, hand-to-hand, etc.), the ability to remove impurities from objects (heavy metals, etc.) Black tea containing fine jade powder with brown rice fiber structure, which is a very thin fiber of granite granules, which has the ability of improving water quality and promoting the growth of animals and plants And to a method of manufacturing the same.

In general, it is well known that jade or tea is a useful material for the human body. The former jade is roughly divided into jadeite and jadeite, and jadeite is a lead fluoride mineral belonging to the pyroxene family. , Soda monoclinic system, hardness is a dense mass like crystal, light is black, cyan, green, transparent or translucent, and jade is generally called jadeite. Point.

Nephrite jade, on the other hand, is a monoclinic whiskey mineral of Inosilicates, and is divided into purgatory and serpentine superbasic purgatory among high-grade surrogate rocks, Their quality is determined by their fine structure, that is, the degree of fineness of hollowstone-amphetite sperm into fastenings and fibers, and it is known that the finer the fiber, the better the quality.

According to a report published in the German authoritative medical literature ("KRAFF der KRISTALLE und der EDELSTEINE" by Mauda Palmer Die Verborgene), jadeite and purgatory are two different ores, which, like most gemstones, contain silicon and oxygen. While jadeite is formed of granular crystals, jadeite is composed of countless crystals and particulate aggregates such as fibers and hairs, and in this respect, jadeite is different from jadeite. Unlike the aluminum composition, it contains three minerals that are beneficial to the human body, namely calcium, iron, and magnesium, which have a great effect on the healing of diseases caused by hypertension, diabetic circulatory disorders, heart disease and kidney disorders. It was announced.

On the other hand, even in Dongbobogam, the classic medicine of oriental medicine, if you put jade into Omiju and Jiyuju, it turns into water, and if you put it in holjangsu, it is good to eat. When eaten at the same size as (체 大), the waste is discharged to the outside of the body, and 1 doeolsul and 1 yujicho (地 楡 草), 1 doe (稻米), 3 egret (白露) mixed together in a copper bowl When the rice is cooked, the oxol turns into water, which is called oxal (玉液) and is called Shinseonokjang (신농 옥장). Shinnongboncho, Dangboncho, and the main cortex According to the jade powder powder to make the enlightenment and taking it not only has the effect of moisturizing the five intestines and exhausting the waste in the body completely, ① it is effective in the digestive system by removing the heat in the stomach, ② Bronchial asthma, fever and heartburn It is good for severity and quenches thirst. ③ If you wear swelling clothes like sesame seeds, your body will fly and become lighter and longer. ④ It will help lubricate the lungs and help the vocal cords. ⑤ Nourishes the hair. It is said to nourish the function of the intestine, and especially to relieve stressful neurological disorders. It is known from the early days that rubbing the skin for a day, the main component of purgatory is consistent with the main elements of the human body and exerts an excellent effect on the human body without side effects.

However, purgatory is known to have excellent medical effects, but due to its scarcity, it is only limited to some personal jewelry ornaments such as necklaces, rings, and bracelets.

On the other hand, black tea is produced using the leaves and fruits of the tea tree, which is an enzymatic oxide of green tea (Camellia Shinensis). In particular, black tea has been widely consumed as a favorite food since ancient times, and in recent years, various pharmacological ingredients contained in black tea are known to be useful for the human body, and are receiving great attention as health supplements. That is, tea contains vitamins C and A and caffeine, which acts as a diuretic, cardiac, and exciting. In particular, polyphenols contained in black tea are widely known to have antidiabetic and heavy metal detoxification effects.

Therefore, the object of the present invention is to develop and pay attention to the medical efficacy of the purgatory and black tea as described above, during the manufacture of black tea mixed with 3 to 10 parts by weight of the nephrite powder having a particle size of 150 to 1000 mesh The present invention is to provide a black tea containing the purgatory powder which has the palatability as well as activating cell function, promoting blood circulation and promoting metabolism, and anticancer drugs, and a manufacturing method thereof.

In order to achieve the above object, the black tea containing the purgatory powder of the present invention is black tea using the leaves and the fruit of the tea according to claim 1, characterized in that the total weight of the black tea is mixed in the ratio of 3 to 10 parts by weight of the purgatory powder do.

The particle size of the nephrite powder is 150 to 1000 mesh, characterized in that the composition ratio (semi-quantitative analysis value (%)) as follows.

Silicon 34

Magnesium 10

Calcium 4.9

Iron 0.23

Aluminum 0.16

Copper 0.17

Cobalt 0.046

Manganese 0.14

Tin 0.024

Beryllium 0.00072

Is 0.0013

Titanium 0.0038

Nickel 0.0028

Chromium 0.0030

Miscellaneous 0

In order to achieve the above object, the method for preparing black tea containing the purgatory powder of the present invention comprises preparing 3 to 10 parts by weight of the purgatory powder having a particle size of 150 to 1000 mesh to the total weight of the black tea. It is characterized by.

In general, the particle size of the purgatory powder is generally 150-350 mesh, but when ductility and touch are to be increased, it is advantageous to use the finer particles of 350-1000 mesh, which are finer particles. If the grain size of the jade powder is smaller than the above range, the roughness of the product is increased, which is not preferable.

In addition, if the addition amount of the nephrite powder is less than 3 parts by weight, the effect due to the addition of nephrite can not be expected, and the higher the addition amount of the nephrite powder improves the inherent efficacy of the nephrite, but if the addition amount of the nephrite powder is too large, the raw material In addition to deteriorating the properties of the product to make the product vulnerable, due to the high price of the nephrite powder, the price of the product is increased, so 10 parts by weight or less of the total weight is preferable.

The nephrite powder used in the above is economically advantageous in terms of cost reduction because it is used to make products such as sculptures from the nephrite and to wash and finely grind the remaining residues.

In addition, the black tea has excellent palatability and pharmacological action, such as ginseng, three hundred seconds. Tea materials such as gamgucha, sansa tea, sasang tea, quince tea, Bokbuncha, Oga tea, Mokhyang tea, thawed tea, clear tea and dermis tea can be added. The above ingredients are known to have effects such as brain circulation, digestion promotion, kidney supplementation, thirst elimination, semen promotion, nerve emulsification, rejuvenation, lower body reinforcement, vision protection, and foot and foot insulation.

1 to 6 show the effect of purgatory used in the present invention on the cell growth of digitalis inana (Digitalis Ianata),

1 is a graph showing the change in the total volume of the culture medium of the cells proliferating in the growth medium,

Figure 2 is a graph showing the change in cell volume,

Figure 3 is a graph showing the change in bio weight of cells,

4 is a graph showing the change in dry weight of cells,

5 is a graph showing the change in pH in cell growth medium,

6 is a graph showing the change in pH of the production medium after the addition of purgatory powder,

7 is a table showing the condition of the water analyzer containing the purgatory water used in the present invention,

8 is a table showing the results of analysis of the water quality containing the purpura used in the present invention.

Hereinafter, a more preferred embodiment of the black tea containing the purgatory powder according to the present invention and a manufacturing method thereof will be described in detail.

Black tea is manufactured by a known method. In other words, they work to select the decayed or insect-eaten ingredients from the leaves or berries of the harvested tea. Selected tea raw materials are soaked in clean water for about 30 minutes or more, and after soaking, the raw materials are shaken and washed with a general sprayer. The water pressure of the sprayer should only be at least 30 Lb / in ² . This is to effectively clean the foreign matter, such as foreign matter on the raw material. After washing the raw material, the sample is drained and steamed at 60 to 100 ° C. for about 2 to 3 hours in a steam cooker to which water is added 5 to 10 times the weight of the sample. Steam treatment is intended to allow the inherent pharmacological components to be eluted to the outside as possible. In addition, it is preferable to use a steam cooker equipped with a water cooler to minimize evaporation of the inherent aroma of tea. The steamed sample is dried and pulverized to obtain black tea. In order to have more palatability from the tea, it may be passed through a roasting process as a far infrared distributor. Black tea obtained by such a known method is mixed with 3 to 10 parts by weight of nephrite powder having a particle size of 150 to 1000 mesh, which is prepared in advance to the total weight of black tea, using a general mixer. The green tea thus mixed is packaged in tea bags in small quantities to simplify drinking.

In addition, the black tea may be heated to a predetermined temperature until it has a predetermined brix degree (Degree Brix) to make an extract (concentrate), and then mixed with the nephrite powder in the range of 3 to 10 parts by weight of the total weight to produce a product. Hereinafter, the embodiment will be described in detail.

<Example 1>

Raw materials such as leaves and sprouts of selected tea are soaked in water for about 30 minutes or more, and after being soaked, they are washed with a sprayer (water pressure is about 30 Lb / in ² or more) while shaking the raw materials. The drained sample is steamed at 60 to 100 ° C. for about 2 to 3 hours in the steam cooker to which water is added 5 to 10 times the weight of the sample. The steamed sample is dried and then pulverized to obtain black tea. 3 parts by weight of nephrite powder having a particle size of 150 to 1000 mesh is mixed with respect to the total weight of the black tea to obtain a product.

<Example 2>

Raw materials such as leaves and sprouts of selected tea are soaked in water for about 30 minutes or more, and after being soaked, they are washed with a sprayer (water pressure is about 30 Lb / in ² or more) while shaking the raw materials. The drained sample is steamed at 60 to 100 ° C. for about 2 to 3 hours in the steam cooker to which water is added 5 to 10 times the weight of the sample. The steamed sample is dried and then pulverized to obtain black tea. 10 parts by weight of nephrite powder having a particle size of 150 to 1000 mesh is mixed with respect to the total weight of black tea to prepare a product. Experimental results for black tea containing the purgatory powder prepared by the above method was the same as the experimental examples (Experimental Example 1 and Experimental Example 2).

<Experimental example 1>

In this experimental example, in order to examine the preference, taste, and the like of black tea added with purgatory powder, the purgatory powder containing black tea prepared in the above example and the black tea containing no purgatory powder were prepared. Quiet) was put in water to drink in accordance with the taste of the testers in the range of approximately 10g or less so that the testers do not know. The results are shown in Table 1 below.

Characteristic Jade salt (the number of the respondents) Control salt (number of respondents) I don't know (number of respondents) Gives a more refreshing taste 28 20 2 Excellent palatability. 30 16 4 I feel a good fragrance 25 21 4

As shown in the above experimental results, black tea added with purgatory powder proved to be superior to or comparable to the control black tea.

<Experimental example 2>

For 50 adult men and women who complain or suffer from symptoms such as headache, numbness, indigestion, and constipation, the black tea containing the purgatory powder prepared in the above example is continued three times a day (about 3 to 5 g) for about 30 days. After drinking, the effect was evaluated by survey. The results are shown in Table 2 below.

Effect Determination Disease No effect Effective Considerable effect Cure effect system headache 2 6 3 One 12 Constipation One 7 5 4 17 Urine 3 4 One 4 12 Indigestion One 4 3 One 9 system 7 (14%) 21 (42%) 12 (24%) 10 (20%) 50 (100%)

As shown in the results of the experiment, when the product is continuously drinking as tea, headache (83%), constipation (94%), urinary pain (75%), indigestion (89%) is alleviated, the effect as a dietary supplement It can be seen that there is.

<Experimental Example 3>

The following experimental examples test the presence of lead, heavy metals, and other toxic components in the jadeite added to the present invention.

<Lead content test> sample Jade minutes Sample property White powder Job number Federal Institute of F.D.A.American Studies IW 091394-1 Way Atomic absorption analysis result Not detected

<Heavy metal> sample Jade powder Sample property White Job number Federal IW 080894-4 Way USP 23 result Not detected

<Inorganic elution test> sample Jade powder Sample property White Job number Federal IW 080894-4 Way As result As

100 g of the sample was extracted with 1 liter of water by autoclave treatment, and the extract was analyzed. This is shown in Table 6 below.

Analyte Results (ppm) Detection limit (ppm) Arsenic (As) ND 0.05 Barium (Ba) ND 0.20 Cadmium (Cd) 0.006 0.005 Chlorine (Cl) ND One Chrome (Cr) ND 0.01 Copper (Cu) ND 0.05 Fe ND 0.10 Pb ND 0.05 Manganese (Mn) ND 0.02 Mercury (Hg) ND 0.0005 Nitrate (NO ₃ ) ND 0.1 Selenium (Se) ND 0.05 Silver (Ag) ND 0.01 Sulfate (SO ₄ ) 1.85 One Zinc (Zn) ND 0.01

* ND = concentration is below or not detected

As shown in the above experimental results, the purgatory powder used in the present invention has no lead, heavy metals, and other toxic components harmful to the human body, so it has been proven stable when added to black tea.

<Experiment 4>

In the present experimental example, the experiment on the effect of the purgatory powder used in the preparation of black tea of the present invention for the experimental sphere (rabbit) was performed as shown in Table 7.

<Primary eye irritation test> sample Jade minutes Sample property Milky powder Way As result As

Method: US Federal Register Vol 43.No 163-1978.8.22 p37359

Experimental procedure: Place 0.1 ml of the extract on the inverted lower eyelid of one eye, and gently hold for 1 second before placing the upper and lower eyelids. The other eye is not treated and observed as a control example. The treated eyes of the six rabbits were left unwashed, and the eyes of the three rabbits were washed with running lukewarm water for one minute immediately after treatment. Prior to treatment with the sample, the eyes were examined in advance by fluorescence and only animals that did not show corneal damage were used for the test. Eye lesions were observed 24 hours, 48 hours, 72 hours, 7 days and 14 days after treatment. The results were shown in Table 8 below.

<Lesion degree of eyes> rabbit time Corneal Opacity Corneal area Iris Price Conjunctival redness Conjunctival Chemosis Conjunctival secretion # 1 fine 24H 0 0 0 0 0 0 48H 0 0 0 0 0 0 72H 0 0 0 0 0 0 7D 0 0 0 0 0 0 score Corneal (0) Iris (0) Conjunctiva (0) # 2 fine 24H 0 0 0 0 0 0 48H 0 0 0 0 0 0 72H 0 0 0 0 0 0 7D 0 0 0 0 0 0 score Corneal (0) Iris (0) Conjunctiva (0) # 3 fine 24H 0 0 0 0 0 0 48H 0 0 0 0 0 0 72H 0 0 0 0 0 0 7D 0 0 0 0 0 0 score Corneal (0) Iris (0) Conjunctiva (0) # 4 fine 24H 0 0 0 0 0 0 48H 0 0 0 0 0 0 72H 0 0 0 0 0 0 7D 0 0 0 0 0 0 score Corneal (0) Iris (0) Conjunctiva (0) # 5 fine 24H 0 0 0 0 0 0 48H 0 0 0 0 0 0 72H 0 0 0 0 0 0 7D 0 0 0 0 0 0 score Corneal (0) Iris (0) Conjunctiva (0)

# 6 fine 24H 0 0 0 0 0 0 48H 0 0 0 0 0 0 72H 0 0 0 0 0 0 7D 0 0 0 0 0 0 score Corneal (0) Iris (0) Conjunctiva (0) # 7wash 24H 0 0 0 0 0 0 48H 0 0 0 0 0 0 72H 0 0 0 0 0 0 7D 0 0 0 0 0 0 score Corneal (0) Iris (0) Conjunctiva (0) # 8wash 24H 0 0 0 0 0 0 48H 0 0 0 0 0 0 72H 0 0 0 0 0 0 7D 0 0 0 0 0 0 score Corneal (0) Iris (0) Conjunctiva (0) # 9wash 24H 0 0 0 0 0 0 48H 0 0 0 0 0 0 72H 0 0 0 0 0 0 7D 0 0 0 0 0 0 score Corneal (0) Iris (0) Conjunctiva (0)

I. Cornea

(A) Opacity-Density (area for detoxification)

Normal-0

Scattered Diffusion Zones-Details of the iris are clearly visible-1

Translucent areas that are easily identified, slightly blurred details of the iris-2

Milky white area, details of the iris are not visible, pupil size is almost indistinguishable

Opaque, no iris visible-4

(B) corneal area

Normal-0

Less than 1/4, but not 0-1

More than 1/4, less than 1/2-2

1/2 or more, 3/4 or less-3

More than 3/4, less than total area-4

Score is A × B × 5, total max = 80

II. Iris

(A) value

Normal-0

Folded above normal, hyperemia, swelling, pericorneal bleeding (these symptoms appear in any size, alone or in combination), the iris still responds to light (slow response is pronounced)-1

Not responding to light, bleeding, total destruction (one or both of these symptoms)-2

Score is A × 5, maximum possible total = 10

III. conjunctiva

(A) red tide (only related to the conjunctiva of the eyelid)

Normal-0

Blood vessels are surely reddened above normal-1

More diffuse, deeper magenta, individual vessels not easily distinguished-2

Spread red like beef-2

(B) chemosis

No Snooze-0

Random swelling phenomenon over normal (including eye blink membrane)-1

Clearly swollen with partial inversion of the eyelids-2

Eyelid half closed and swollen-3

Eyelid half to full closed and swollen-4

(C) secretion

Normal-0

Any phenomenon other than normal (not including small amounts observed at the tip of the inner eye of normal animals)-1

Secretions only on the eyelids and eyebrows adjacent to the eyelids-2

Significant area around the eyes-3

Score (A + B + C) × 2, total max = 20

CONCLUSIONS: This sample was determined to be free of eye irritation, and therefore, it was found to be suitable for use in eye-related products.

<Primary skin irritation test> sample Jade minutes Sample property White powder Way As result As

Method: US Federal Register Vol. 43, No. 163

CTFA (The Cosmetic, Toiletry and Fragrance Association, Washington, D.C.)

Experimental procedure: Patch testing was performed on the skin of the white rabbit and the original skin. The hair was cut short at the back and sides. Two areas, about 10 cm away, were selected as patch locations. One area is made of four small epidermal incisions in the patch area (2 of which are tic-tac-toe vertically perpendicular to the other) and one-inch patches of surgical gauze. Is attached using a thin rubber band and adhesive tape. Samples are introduced into each site in amounts of 0.5 ml (g) under the patch. The body of the animal is wrapped with a rubberized cloth for 24 hours of exposure. The animal is immobile during the exposure time. After removing the patch, the results of evaluating the reactions at each site based on the scores described below were as follows.

Evaluation of Skin Response

Erythema and crust formation

No erythema-0

Very weak erythema (recognizable barely)-1

Properly identified erythema-2

Moderate to severe erythema-3

Severe erythema (beat red) to slight crust formation (deep damage)-4

Edema

No edema-0

Very mild edema (recognizable barely)-1

Slight edema (it is obvious that the edges of the area are prominent)-2

Moderate edema (approximately 1 mm up)-3

result

rabbit 24 hours 72 hours Original skin Worn skin Original skin Worn skin ER ED ER ED ER ED ER ED #One 0 0 0 0 0 0 0 0 #2 0 0 0 0 0 0 0 0 # 3 0 0 0 0 0 0 0 0 #4 0 0 0 0 0 0 0 0 # 5 0 0 0 0 0 0 0 0 # 6 0 0 0 0 0 0 0 0

Conclusions: This sample was determined to have no skin irritation. It has thus been found to be suitable for use in skin treatment products and the like.

<Acute oral toxicity [(0.5 g) / 100 g body weight] experiment> sample Purgatory powder Sample property White powder Way As result Same as Table 19 below

rat gender Initial weight (g) Dosage (ml) Lag weight (g) Toxicity One F 207 1.0 219 none 2 F 211 1.1 226 none 3 F 215 1.1 225 none 4 F 208 1.0 217 none 5 F 200 1.0 213 none 6 M 227 1.1 235 none 7 M 216 1.1 224 none 8 M 205 1.0 218 none 9 M 210 1.1 224 none 10 M 212 1.1 221 none

Preparation of Samples: A portion of the sample was placed in sterile distilled water and boiled for 10 minutes to extract, and the extract was administered by intubation in an amount of 0.5 g per 100 g of body weight.

CONCLUSIONS: The sample meets the criteria for no oral toxicity.

<Experiment 5>

This experiment example is the process and result of the experiment conducted by Inha University bioinformatics engineering laboratory to investigate the various effects of purgatory powder on the living body.

In this experiment, 70% of the constituent elements of the human body are water, so it is determined that there will be many relations between the change of water and the effect on the human body, and then the effects of the hard water on the growth of Digitalis plant cells. Experiments on the effects were performed.

A. Hard water change

<Experiment 1>

100 ml of artificially-made hard water was transferred to each of the four flasks by 50 ml, and purgatory was placed on the bottom of two flasks for about 10 minutes.

To determine the change in hardness, a titration method using ethylene diamineacetic acid (hereinafter referred to as "EDTA" titer 2.9412) was used. 1 ml of a buffer solution of pH 10, EBT was used as an indicator.

The change in hardness was measured by the amount of EDTA until the moment when the color of the hard water was changed by EDTA. At this time, the color returned to its original state over time, but in this experiment, the measurement was made based on the changing moment.

<Result 1>

At 100ppm of hard water, it takes 1.70ml of EDTA.

It takes 1.25ml of EDTA after purgatory test, so the hardness of 100.00ppm is lowered to 73.53ppm. That is, the hardness of 100.00 ppm was softened 26.47%.

<Experiment 2>

200 ml of 100ppm hard water made in the same manner as in Experiment 1 was transferred to a beaker to immerse the purgatory.

After about 30 minutes, the water in the beaker was divided into three flasks to measure the change in hardness. In this experiment, the hardness was measured by adding EDTA until the color of hard water did not change any more after the color of hard water changed by EDTA.

<Result 2>

Each 100ppm hard water before purgatory experiment

89.62 ppm

91.19ppm

89.62 ppm

Average 90.14ppm, thus 9.9% hardness decrease

<Experiment 3>

This experiment was conducted with tap water. Tap water was placed in a container and transferred to six flasks, and purgatory was placed at the bottom of three flasks, and hardness was measured after about 5 minutes.

<Result 3>

* Tap water without purgatory

97.48 ppm

97.48 ppm

97.48 ppm

Average 97.48 ppm

* Tap water after purgatory experiment

91.19ppm

91.19ppm

91.19ppm

Average 91.19ppm therefore, hardness decrease of 6.5%

I. Effect of Purgatory on the Proliferation of Digital Plant Suspensions of Digitalis lanata

① Effect of Purgatory on the Growth of Digitalis lanata Cells in Growth Medium

1 is a graph showing the change in the total volume of the culture medium of the cells proliferating in the growth medium for 11 days, Figure 2 is a graph showing the change in the cell volume, in proportion to the increase of the cells growing over time As the depletion of and the evaporation of the medium occur, the volume change of the whole culture reflects these factors. In particular, humidity and temperature in the atmosphere are closely related to the evaporation of the medium, and such factors also affect the cell growth rate.

As shown in the figure, the use of purgatory shows that the volume of the total culture solution is slightly decreased compared to the control group which is not used. This is because the use of purgatory rather than the control group has a faster cell growth rate against the evaporation of the medium or the depletion of nutrients, and thus, it is possible to offset the rapid decrease of the depleted medium to some extent. This phenomenon is observed in the case of continuous use of purgatory from day 4 to day 11 after inoculation of the cells as shown in Figure 2 measuring the volume change of the sunk cells only increase the volume of the cell than the unused control group This fact can be estimated. Therefore, the use of nephrite showed a faster cell growth rate than the control without the nephrite. These results can be said to be the result of delaying the decrease of the total volume than the control by offsetting the decrease in the total culture volume due to nutrient depletion and medium evaporation. In addition, the result of comparing the volume of the increased cell alone is also observed to show a tendency to gradually increase the cell volume after 4 days, it is thought that can support this result.

Cell proliferation and dry weight are the most common measures of cell proliferation. In general, the growth curve of all cells is represented by the S-shaped curve, which can be classified into four stages. Lag phase, which is a period of adaptation to the medium, in which there is no cell proliferation and no mass growth, and an exponential curve showing a steep slope due to a mass increase at a rapid proliferation rate after this period. phase), the stationary phase, which has a maximum growth to a certain extent, and is slow to eat, and shows no mass increase, and the cell ruptures due to the depletion of nutrients, the release of toxic components, and the saturation of cell density. It can be divided into a dead phase where the total cell volume or mass decreases due to the tendency to die.

3 and 4 are the results of measuring the cell biomass and dry weight.

3 follows the growth curve of these four stages. In the case of using jade, after 7 days, the growth rate was slightly higher than that of the control without jade, and the growth stopped until 10 days, unlike the control showing the decrease in cell biomass. have. This is remarkable in that cell growth is continued even when the concentration of nutrient is almost depleted and the concentration in the medium becomes zero compared with the change in the concentration of glucose, a nutrient that acts as the main metabolic substrate in the medium. It is common to show a tendency to stop the growth and decrease the growth rate at the point of depletion of the influenced ingredients, like the control without purgatory, but the effect of purgatory on cell growth is different from purgatory It is assumed that there is. This result is also shown in Figure 4 after 3 days to show a cell growth proliferating at a higher rate than the control, and after 9 days the same phenomenon as in Figure 3. As a result, cells grown close to purgatory in growth media showed a better effect on cell growth than controls that did not. Digitalis lanata plant cells, which have a growth limit of about 10 days, are maintained in a stable state without cell necrosis without exchanging with new medium even after 10 days when cultured with purgatory. Rather, it is expected that cell proliferation may be an advantageous method for optimizing cell growth and concentration in the production process for the production of useful substances.

② Effect of Purgatory on Changes in Medium pH

Figure 5 is a graph showing the pH change in the cell growth medium, Figure 6 is a graph showing the pH change of the production medium after the addition of purgatory powder.

Figure 5 is a measure of the change in pH of the medium in the case of using the purgatory in the growth medium and the control group not used. The control group shows a general tendency of pH in the medium applied to the plant cells, which gradually decreases over time and is maintained at a certain level. However, when using purgatory, it showed a similar trend to the control group until day 7, and gradually increased. This phenomenon is extreme in the result of FIG. 6 showing the result in the production medium in which the purgatory powder was added directly to the culture medium. .

The control group without added jade powder showed a consistent drop in pH, whereas the addition of jade powder showed a tendency to maintain the value to some extent. It is assumed that it acts as a cause.

③ Results of analysis of water quality for purgatory

FIG. 7 is a table showing the condition of the water quality analyzer containing the nephrite powder used in the present invention, and FIG. 8 is a table showing the analysis results of the water quality containing the nephrite powder used in the present invention.

The data from FIGS. 7 and 8 are summarized in Table 13 to Table 16. Table 13 shows the measurement of each analysis item for Sample 1 after 48 hours in which 20 g of nephrite lump was added to the solution. In Table 13, there is almost no change in each measurement item, and the effect of purgatory is hardly seen.

Table 14 shows the measured values for each analysis item in Sample 2 after 48 hours of placing 20 g of purgatory lumps outside the solution.

Table 16 also shows the measured values for each analysis item in Sample 4 after 48 hours after placing the purge powder outside of the solution. However, in the case of Sample 3, the data measured for each analysis item of the sample 48 hours after 20 g of purgatory powder was added to the solution is shown in Table 15. There are many changes that can be seen in Table 15. The change is as follows.

Cr, Pb, Ni, and Co, which are harmful to the human body, changed significantly after 48 hours. Cr was removed in the absence of purgatory powder, that is, 45.30 mg before the reaction was removed to 0 after 48 hours in the purifying powder, and Pb was also completely removed from 13.76 mg to zero. Ni decreased from 51.8 mg to 1.733 mg and Co also decreased from 52.69 mg to 11.94 mg. Mg, one of the necessary elements of human body, increased from 48.36mg to 55.74mg, but increased in combination with urea itself, but there was no change in hardness.Hydrogen concentration of distilled water was pH 3.5, but after reaction, pH6.8 It changed to neutral, and its conductivity decreased drastically. The adsorption force is physically large, about 3-4 mmol.q (equivalent) per gram.

Analytical results before and after the reaction in solution Purgatory lumps (purg of jade in solution) (Sample 1): Purgatory lumps in solution (20 g) Item Before the reaction After reaction (48hr) Change pH 3.5 3.82 - Ni 51.8 mg 51.75mg - Co 52.69mg 52.54mg - Cr 45.30mg 43.88mg - Mg 48.36mg 48.59 mg - Pb 13.76 mg 13.90mg -

Results of analysis of purgatory lumps outside the solution. (Sample 2): lump of purgatory jade (20g) Item Before the reaction After reaction (48hr) Change pH 3.5 3.65 - Ni 51.8 mg 48.92mg - Co 52.69mg 49.83mg - Cr 45.30mg 41.23mg - Mg 48.36mg 47.97mg - Pb 13.76 mg 15.1mg -

Analysis result when adding purgatory powder to the solution (Sample 3): Purgatory powder (20g) Item Before the reaction After reaction (48hr) Change pH 3.5 6.8 +3.3 Ni 51.8 mg 1.733 mg -50.06mg Co 52.69mg 11.94mg -40.75mg Cr 45.30mg 0mg -45.30mg Mg 48.36mg 55.74mg + 7.38mg Pb 13.76 mg 0mg -13.76 mg

Analysis result when purgatory powder was placed outside the solution (Sample 4): Purgatory powder outside the solution (20g) Item Before the reaction After reaction (48hr) Change pH 3.5 3.7 - Ni 51.8 mg 51.53 mg - Co 52.69mg 52.55mg - Cr 45.30mg 43.0mg - Mg 48.36mg 48.39mg - Pb 13.76 mg 14.29 g -

As described above, the digitalis lanata plant suspension cells cultured for one week with nephrite close showed proliferation of about 30% or more without purgatory. This is a rare proliferative result even in the various experiments conducted for the proliferation effect on conventional Digigitalis lanata plant cells for high concentration culture. In addition, experiments on how purgatory water affects hard water showed that soft water softened hard water without contact with water.

Particularly, in the component analysis experiments on water using distilled water, precipitation of nephrite was carried out. After 48 hours, in the component analysis, the pH was increased, the Ni and Co components were reduced, the Cr and Pb were removed, and the heavy metals were removed. An unusual phenomenon was observed that led to the phenomenon of.

As such, purgatory powder according to the present invention has not yet been clarified theoretically, but changes the hardness of water without contact with water, and also proliferates more than 30% of the digitalis innata plant suspension cells. The removal of heavy metals such as reduction of Ni, Co, Cr, Pb and other harmful substances is believed to be due to the radiation effect of the wavelength of the purgatory powder and the ionic reaction by minerals contained in the nephrite. These facts will be proved by the efficacy shown in the product of the present invention described above, and also the results of the experiments in the case of black tea containing the purgatory powder of the present invention due to the characteristics of the black tea and due to the influence of the purgatory heavy metals This suggests that there will be effects such as removal of water, control of pH and softening of hard water.

As described above, according to the present invention, in addition to the unique properties such as detoxification and antibacterial action of black tea synergistically, purgatory by the purgatory Factors such as activation of water molecules, promotion of blood circulation, and activation of cell functions are generated, and have an excellent effect as a dietary supplement that promotes metabolism of the human body.

Claims (3)

The black tea containing the nephrite powder, characterized in that 3 to 10 parts by weight of the nephrite powder having a particle size of 150 to 1000 mesh is included in the total weight of the black tea. The black tea containing the purgatory powder according to claim 1, wherein the purgatory powder has a composition ratio [semi-quantitative analysis value (%)] as follows. Silicon 34 Magnesium 10 Calcium 4.9 Iron 0.23 Aluminum 0.16 Copper 0.17 Cobalt 0.046 Manganese 0.14 Tin 0.024 Beryllium 0.00072 Is 0.0013 Titanium 0.0038 Nickel 0.0028 Chromium 0.0030 Miscellaneous 0 In the preparation of black tea, a method of producing black tea containing jade jade powder, characterized in that 3 to 10 parts by weight of the jade powder having a particle size of 150 to 1000 mesh is mixed with the total weight of the black tea.