KR100227469B1 - Portable can of gaseous nophrite - Google Patents

Abstract

SUMMARY OF THE INVENTION An object of the present invention is to provide a gas purgatory breathing can in which a large amount of minerals (purg) present in purgatory ores can be inhaled at all times while being filled in a can container such as oxygen.

The present invention is filled in the can body with a gaseous purgatory material containing a large amount of minerals useful for promoting metabolism at a predetermined pressure, such as oxygen, the pressure that can be sucked when opening the can body It is to be discharged like oxygen. This makes it possible to inhale oxygen containing minerals, that is, purgatory substances together with oxygen, and to purify the five intestines and promote metabolism by purifying impurities in the body by the purgatory substances sucked in addition to the oxygen supply. To have.

Description

Portable gas purifying cans for purifying gaseous phase

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a portable gas purifying can for suction, and in particular, is used to mine jades having a very fine collagen fiber structure of hollow-glazed-Yangseok-based fibers. It can prevent the human body disease (depression, dizziness, headache, etc.), remove impurities (heavy metal, etc.) by filling the canister with the gaseous purgatory material that circulates in the purgatory body mine. The present invention relates to a portable gaseous purgatory inhalation can that can be applied to a wide range of human body metabolism promotion.

Generally, jade is divided into jadeite and nophrite grade, and jadeite is a mineral in lead fluoride group belonging to pyroxene family. ) Is a monoclinic system, the hardness is a dense mass like crystal, and the light is black, cyan, green, transparent or semi-transparent. Usually, jade refers to this jadeite.

Purgatory is an inosilicates monolithic whiskey mineral, which is divided into purgatory and serpentine superbasic purgates in high-quality surrogate rocks, and their quality is characterized by their fine structure, Hollow granite-Yang Ki-seok is determined by the speed of fastening and fiber, and it is known that the thinner the better the quality. (Mining Promotion 1993 New Year's Issue: Korea Mining Promotion Corporation)

On the other hand, according to a report published in the German medical literature ("KRAFT der KRISTALLE und der EDELSTEINE" by Mauda Palmer Die Verborgene), jadeite and jade are two different ores, which, like most gemstones, contain silicon and oxygen, but jadeite Silver granules are made of granular crystals, while purgatory is composed of countless crystals such as fibers and hair and fine aggregates. Especially, purgatory stones are three minerals that are beneficial to the human body, unlike natrium and aluminum in jadeite. In other words, calcium, iron, and magnesium as the main ingredient has been reported recently to have a great effect on the healing of diseases caused by high blood pressure, diabetic circulatory disorders, heart disease and kidney disorders when worn purgatory.

Also, in Dongbobogam, a classic of oriental medicine, jade is added to Omiju and Jiyuju, and it turns into water, and it is good to eat when it is put in Honjangsu. When eaten in the same size as 大, the waste is discharged to the outside of the body, 1 oxol, 1 jijicho (地 楡 草), 1 bream (1 米), and 3 egret (白露) are mixed together and put in a copper bowl. If you build a temple, the oxol turns into water, which is called oxal (玉液) and is called Shinseonokjang (神仙 玉 漿), and it is called Shin Nongwoncho, Dangboncho, and Boncho Rivers. According to the jade powder to make the enlightenment and taking it not only has the effect to make the five intestines rich and to completely discharge the waste in the body, but also to remove the heat in the stomach (胃) is effective in the digestive system , Bronchial asthma and fever with heartburn Good and thirst quenching, sesame-like blouse, makes the body lighter and longer to fly, lubricates the lungs, helps the vocal cords to nourish the throat, nourishes the hair, and functions of the intestines It also helps to relieve stressful neurological disorders.In addition, it is good to eat white jade powder when the muscles are tense and the rats fly, and rub the wound with purgatory for several days when there are scar marks on the face and body. Purifying scars, the main ingredient of purgatory has been known for its excellent effects on the human body with few side effects.

Although purgatory has been known to have such excellent medical effects due to its scarcity, it has been limited to its use as some jewelry for jewelry, such as necklaces, rings, and bracelets. It is necessary to pay close attention to those who have long experience and cannot work on it, and because it is expensive, it is economically disadvantageous and there is almost no development to use purgatory generally. Therefore, its research and development is urgently required. It's time to be.

Applicant paying attention to the excellent medical efficacy of the purgatory as described above, the applicant has a number of domestic and foreign patents other than "glass products containing jade powder and its manufacturing method" (Patent No. 112812).

In addition, the Applicant pays attention to the efficacy of the purgatory, by allowing the general public to directly inhale the oxygen containing a large amount of purifying circulating in the purgatory mine into the human body through the respiratory tract at any time It is to strengthen.

On the other hand, there is a "safety breath oxygen can" disclosed in the Republic of Korea Patent Publication No. 89-12677 as a can that allows the general public to breathe in the human body. There are oxygen breathing cans that are packaged and sold in a can of a certain size for the purpose of supplying oxygen to those who need oxygen.

However, the source technology has the advantage that the general public can easily carry and breathe oxygen whenever needed, but the contents are limited only to the oxygen supply, so it does not have a great effect on the elderly or the patient. to be. In particular, it is necessary to provide the elderly and the patient with a substance for promoting physical function to restore the function of the body in addition to oxygen supply.

Therefore, the object of the present invention was developed in the light of the above points, and the result of intensive research. The gaseous purgatory material collected from the jade ore is pressurized and filled in a predetermined canister such as oxygen to be sucked when necessary. One purpose is to provide a portable purgatory breathing can that allows users to obtain the superior medical benefits of purgatory as well as oxygen supply.

Features of the present invention for achieving the above object, in the can for breathing, can body; And a gaseous purifying substance filled with the can main body such as oxygen at a predetermined pressure and containing a mineral substance which is exhausted with oxygen at an inhalable pressure when the can main body is opened. Is in a portable purgatory can.

)로 조성됨이 바람 직하다.The gaseous purgatory material has a ratio of

It is desirable to be composed of).

In addition, the purgatory substance in the gaseous phase is collected and charged as it is generated in the purifying ore.

The can body further includes a suction device coupled to the can body to be detachably attached to the can body and providing a suction path through which a gaseous filling discharged when the can body is opened through the human respiratory tract. It is preferable to.

In addition, the suction device is a suction hose and the suction hose to form a bellows-type cap coupled to surround any one side of the can body, the suction hose formed integrally with the bellows-type cap to contact the outside of the can body with the outside It is formed at one end of the inlet comprises a suction port to be sucked through the suction pipe of the human body.

1 is an exploded perspective view of a portable gas purgatory suction can according to the first embodiment of the present invention,

2 is a cross-sectional view taken along the line I-I of FIG. 1,

3 is a perspective view of a portable gaseous purgatory suction can according to the second embodiment of the present invention,

4 is a perspective view of a portable gaseous purgatory suction can according to a third embodiment of the present invention.

A graph showing the results of observing the change in the total volume of the culture medium of Digitalis lanata cells growing in the growth medium of Figure 5 (Experimental Example 5),

6 is a graph showing the change of Digitalis lanata cell volume (Experimental Example 5),

7 is a graph (Experimental Example 5) showing the change in the bio weight of the cells,

8 is a graph showing the change in dry weight of cells (Experimental Example 5),

9 is a graph showing the pH change of the Digitalis lanata culture medium (Experimental Example 5),

10 is a graph showing the pH change of the production medium after the addition of purgatory powder (Experimental Example 5),

11 is data outputting the condition conditions of the analyzer (Experimental Example 5),

12 is the output data of the analysis result (Experimental Example 5).

* Explanation of symbols for main parts of the drawings

10, 40: can body 12: tab for opening

20: suction device 22: bellows-type cap

24: suction path 26: suction hose

28: suction port 29: fixed band

30: purgatory substance in gaseous phase

Hereinafter, with reference to the accompanying drawings will be described in detail a more preferred embodiment of the portable purgatory suction can according to the present invention.

FIG. 1 is an exploded perspective view of the portable purgatory suction can according to the first embodiment of the present invention, and FIG. 2 is a cross-sectional view of the bonded state along the line I-I of FIG.

As shown in the figure, a purgatory suction can according to an embodiment of the present invention includes a can body 10 that is hermetically sealed.

A tab 12 for opening the inside of the can body 10 to the outside is provided at one side of the can body 10 to be opened. From the opening portion 14 formed when the tab 12 is pulled out of the can body 10, the contents of the gaseous phase pressurized in the can body 10 are applied to the pressure that the tab 12 has itself. Is quickly discharged to the outside. The contents filled in the can body 10 are inhalable gaseous substances which will be described later in detail.

The contents discharged from the can body 10 may be inhaled through the user's respiratory organ, that is, through the mouth or nose, but it is preferable to use the inhalation device 20 described later.

The suction device 20 is sold by forming one set with the can body 10 and a bellows formed to be hermetically coupled to any one end of the can body 10. The mold cap 22 is provided. Since the cap 22 is formed in a bellows shape, the cap 22 may be stretched and contracted from the can body 10, and may be elastically coupled as a shape surrounding the periphery of the can body 10 with its own elastic force. The bellows-type cap 22 can be used in conjunction with the can body 10 after the tab 12 is opened, so that the contents discharged through the opening 14 of the can body 10 are the bellows-type cap 22. After passing through the suction hose 26 which is formed integrally with the) and provides the suction passage 24, it is discharged to the outside through the suction port 28 coupled to one end of the suction hose 26. The suction hose 26 is formed to be inserted into the can body 10.

The contents discharged through the suction port 28 can be sucked into the human body through the respiratory tract of the human body. The inlet 28 is formed in the shape of a mask to be easily positioned in the human respiratory organ (mouth or nose, etc.), and the mask type inlet 28 is formed using a fixing band 29. I can fix it firmly on a face.

The contents of the can main body 10 are filled with the air present in the jadeite ore as it is, and the air containing the purifying substance 30 and oxygen in the gaseous phase can be manufactured and supplied. Is filled at a predetermined pressure, that is, at a pressure of approximately 8-10 kg / cm 2. The can body 10 is preferably manufactured in a size of approximately 500 or 600 ml for easy portability. This is a dose of about 1-3 minutes when an adult breathes repeatedly for about 2-4 seconds once.

)로서 조성된다.The contents filled in the container of the can main body 10, that is, the purgatory substance 30 in a gaseous state may have a component ratio as follows, such as oxygen.

Is formed as

* Other substances in the above are nitrogen, argon, carbon dioxide, neon, helium, krypton and xenon.

3 is a perspective view of a portable purgatory suction can according to a second embodiment of the present invention.

In this embodiment, the can main body 40 is provided with a compression nozzle 42 for discharging the purgatory material filled therein. The purging material discharged together with oxygen when the compression nozzle 42 is compressed is a suction hose ( 52 and a suction device 50 having a mask-shaped suction port 58 allow users to simultaneously inhale through the nose and mouth. The suction port 58 is provided with fixing bands (59a, 59b) to be hooked to the user's ear. Other configurations and operations are the same as the above embodiment, and a detailed description thereof will be omitted.

4 is a perspective view of the portable purgatory suction can according to the third embodiment of the present invention, and the same parts as those of the second embodiment are denoted by the same reference numerals in order to avoid duplicate explanation.

Unlike the first and second embodiments, the present embodiment uses the suction pipe 60 installed to protrude from the compression nozzle 42 so that the purifying substance filled in the can body 40 can be sucked into the mouth. will be. Other configurations and operations are the same as those of the first and second embodiments, and detailed description thereof will be omitted.

)에 비하여 훨씬적고, 그 반면에 연옥물질의 함유량이 많기 때문에 모두(冒頭)에서 설명 한바와 같은 이유로 인체내에 호흡시 산소이외에 공급되는 연옥물질이 체내에 존재하는 불순물등을 정화하여 신체의 기능을 강화하는 의학적 효능이 있음을 알수가 있었다.Through the first to third embodiments, the male and female adults, especially those who have a disease, who have randomly selected the air containing the gaseous purgatory substance filled in the can bodies 10 and 40 continuously inhale for a long time. As a result, the content of nitrogen in the can body 10 and 40 collected in the jadeite and filled in the can body 10, 40, that is, the amount of nitrogen contained in the air present inside the general light About 70

It is much less than) and on the other hand, because of the high content of purgatory substances, for the same reason as explained in all cases, purifying purgatory substances other than oxygen when breathing in the human body purifies impurities in the body It can be seen that there is a strengthening medical effect.

Such medical efficacy may be confirmed from the following experiments performed using the product and the jade material excavated from such light.

Experimental Example 1

In this experimental example, the 50 mi products manufactured from the present invention were repeatedly inhaled at about 30 minutes intervals for at least 20 days for 50 adult men and women who complained of symptoms such as headache, insomnia, numbness and indigestion. The effect was evaluated by a questionnaire. The results are shown in Table 1 below (Department of Oriental Hospital of Daejeon University).

), 불면증(81

), 손발저림(90

), 소화불량(89

)이 완화되었음을 알 수 있다.Inhalation of the product according to the invention, as shown in the above experimental results, headache (80

), Insomnia (81

), Numbness (90)

), Indigestion (89

) Is alleviated.

Experimental Example 2

In this Experimental Example, the experiment was commissioned by the Korea Food Research Institute to investigate the effects on the reproduction and growth rate of rats when treated with a gaseous purgatory material used in the product of the present invention and corn with precipitated purge powder. Was carried out. Details are as follows.

Nine-week-old Sprague Dawley rats were prepared for 30 males and females. The rats of group A were allowed to drink tap water in the cage containing the air containing the gaseous purgatory material, and the rats of group B were allowed to drink the supernatant separately after dipping the nephrite powder (2mg / ml tap water). We did not put us in the supply of material. In the C group, the control group had no air or purgatory water containing purgatory substances. All treatments were seeded for 9 days and then males were isolated and slaughtered for sperm concentration and vitality testing. The results are shown in the table below.

, C 처리군에서 80

를 나타내었고, 분만새끼수는 비록 대조군에서 6 - 8마리 더 많았지만 숫 새끼수는 A,B 처리군에서 3마리씩 더 많았다. 종부후 분만까지 소요되는 일수는 A 처리군이 유의하게 짧았다. 또한, 기상의 연옥물질을 공급한 우리에서 처리한 A군의 경우 정자 농도와 정자의 활력이 우수하였다.As a result, the results of delivery were better than those of the control group in the A group and A B group treated with the gaseous nephrite used in the present invention. In other words, the delivery rate was 90 in A and B treatment groups.

80 in C treatment group

Although the number of pups was 6-8 more in the control group, the number of male pups was 3 more in the A and B treatment group. The days to postpartum delivery were significantly shorter in the A treatment group. In addition, sperm concentration and vitality of sperm were excellent in the group A treated in the cage fed with gaseous nephrite.

However, the effects of the simultaneous treatment of gaseous nephrite and maize were not studied in this experiment.

In conclusion, the results suggest that jade material and corn affect the reproduction and growth of rats, inhalation of gaseous jade material or acorn treatment group showed better reproduction and growth index. It is expected that a lot of valuable research will be performed on these phenomena. However, these results are considered to be valuable as basic data for improving reproductive efficiency and enhancing sperm concentration and motility.

Experimental Example 3

This experiment was performed by the Korea Food Research Institute to investigate the effects of various purgatory substances on the symptoms of streptozotocin-induced diabetes in rats. Details are as follows.

32 spragdoli mice weighing 252-292 grams were intraperitoneally administered ST (streptozotocin) (35-40 mg / kg body weight dissolved in 0.1M citric acid in pH 4.0) to cause diabetes It was. Seven days after ST administration, using only europaper (Eiken Chemical Co., Ltd., Japan), only urine glucose over 250 mg / dl mice were selected and placed in 5 groups per treatment group for 50 days. Animals in group A were intraperitoneally injected daily with 1.0 ml of purifying distilled sap (pH 6.40). Group B was raised in a cage fed with meteorological jadeite. The group C was fed daily with supernatant of nephrite corns after precipitation of nephrite powder, and the group D was administered only ST as a control. Experimental feed was fed commercially available feed, the breeding method was in accordance with the usual practice. Mice were sacrificed at the end of the experiment (50 days) and blood was collected from the abdominal aorta. Blood was left at room temperature for 30 minutes and then centrifuged at 5,000 rpm for 15 minutes to obtain plasma. The weights of organs, such as isolated liver and kidneys, were weighed and recorded. Plasma β-hydroxybutyrate (HBA), free fatty acids (FFA), cholesterol, HDL-cholesterol and triglycerides of plasma were measured. The HBA content was quantified by measuring the increase in absorbance (OD) at 340 nm of NADH produced by the oxidation reaction with β-hydroxybutyrate dehydrogenase, and the blood lipid was measured in a clinical laboratory kit (Eiken Chemical Co., Ltd., Japan).

p <0.05

^a β-hydroxybutyrate (ketone body)

^b free fatty acids

^c total cholesterol

^d triglyceride (neutral lipid)

^e high density lipoprotein cholesterol

Body weight increased in the C group fed the nephrite corn, while it was decreased (B, D) or maintained in the other groups (A). In diabetic rats, renal hypertrophy is known to be a common symptom. Similar trends were observed in this study, and the renal weight of group C was the lowest among the treatment groups except the control group. Hepatomegaly was also seen due to diabetes, but liver weight was the lowest in rats fed corn. The concentrations of blood glucose, ketone bodies and FFA were highest in the order of A, B, C, and D treatment groups. Plasma cholesterol and triglyceride concentrations showed the same trend (A> B> C> D). From the above results, rats fed the nephrite corn (group C) showed relatively lower values of all the indexes than the other two experimental groups, and blood cholesterol and FFA concentrations were lower than those of the control group without the nephrite treatment.

Blood cholesterol (TC) and neutral lipid (TG) concentrations were generally higher in the order of A, B, C, D treatment groups. When the glycemic index was determined by the post-prandial blood glucose response, the experimental results differed slightly depending on the researcher. However, in this experimental example, a commercially available complete formula (TMR) without considering the glycemic index of cereals in the experimental feed was considered. Even though they were fed, the treatment group (group C) fed agate improved the hyperlipidemia, one of the symptoms of diabetes. HDL-cholesterol content did not differ between treatment groups.

In conclusion, when the meteorological purifying substance and purge water were fed to streptozotocin-induced diabetic rats, not only the weight loss but also the renal or hepatic hypertrophy and the hyperlipidemia, a type of diabetes mellitus, were improved.

Experimental Example 4

In this Experimental Example, experiments were carried out on the effect of purgatory components such as gaseous purgatory substances used in the present invention on the experimental spheres (rabbits) (the FDA-approved laboratory).

Method: US Federal Register Vol 43.No 163-1978.8.22 p37359

Experimental procedure: 0.1 ml of extract is placed high on one inverted lower eyelid, and then gently held for 1 second before placing the upper and lower eyelids. The other eye is observed as a control without treatment.

The treated eyes of the six rabbits were left unwashed, and the eyes of three rabbits were washed with lukewarm water for one minute immediately after treatment. Prior to treatment with the sample, the eyes were examined in advance by fluorescence and only animals that did not show corneal damage were used for the test. Eye lesions were observed 24 hours, 48 hours, 72 hours, 7 days and 14 days after treatment.

result :

I. Cornea

(A) Opacity-Density (area for detoxification)

Normal-0

Diffuse Area Scattered—Details of the iris are clearly visible -1

Translucent areas that are easily identified, slightly blurred details of the iris -2

Milky white area, details of iris are not visible, pupil size is almost indistinguishable-3

Opaque, no iris visible-4

(B) corneal area

Normal-0

Less than 1/4, but not 0 -1

More than 1/4, less than 1/2 -2

1/2 or more, 3/4 or less -3

More than 3/4, less than total area -4

Score is A × B × 5, total max = 80

II. Iris

(A) value

Normal-0

Folded above normal, hyperemia, swelling, pericorneal congestion (these symptoms appear in any size, alone or in combination), the iris still responds to light (a slow response is evident) -1

Not responding to light, bleeding, total destruction (one or all of these symptoms) -2

Score is A × 5, possible maximum = 10

III. conjunctiva

(A) red tide (only related to the conjunctiva of the eyelid)

Normal -0

Blood vessels are surely congested above normal -1

More diffuse, deeper magenta, individual vessels not easily distinguished -2

Spread red like beef -2

(B) chemosis

No pour -0

Random swelling phenomenon -1 above (including the blinking film)

Obviously swollen with partial inversion of the eyelids -2

Eyelid half closed and swollen -3

Eyelid half to full closed and swollen -4

(C) secretion

Normal-0

Any phenomenon other than normal (not including small amounts observed at the inner eye tip of normal animals) -1

Secretion wet only on the eyelids and the eyebrows adjacent to the eyelids -2

Occupies a significant area around the eyes -3

Score (A + B + C) × 2, total max = 20

CONCLUSIONS: This sample was determined to be free of eye irritation and, therefore, was found to be suitable for use in eye cosmetic products.

Method: US Federal Register Vol. 43, No. 163

CTFA (TheCosmetic, Toiletry and Fragrance Association, Washington, D.C.)

Experimental procedure: Patch testing was performed on the skin of the white rabbit and the original skin. The hair was cut short in the back and sides. Two areas, about 10 cm apart, were selected as patch locations. One area is made of four small epidermal incisions in the patch area to exfoliate the skin (two of which are in a "tic-tac-toe" fashion, perpendicular to the other). Patches of 1 inch each of the surgical gauze are attached using a thin rubber band and adhesive tape. Samples are introduced into each site in amounts of 0.5 ml (g) under the patch. The body of the animal is wrapped with a rubberized cloth for 24 hours of exposure time. The animal is immobile during the exposure time. After removing the patch, the results of evaluating the reactions at each site based on the scores described below were as follows.

Evaluation of Skin Response

Thorax and scalp formation

No nevus -0

Very weak erythema (recognizable) -1

Well-Identified Erythema -2

Moderate to severe erythema -3

Severe erythema (beat red) to slight crust formation (deep damage) -4

Edema

No edema-0

Very weak edema (recognizable enough) -1

Slight edema (the edges of the area are obvious) -2

Moderate edema (approximately 1 mm rise) -3

result :

Conclusions: This sample was determined to have no skin irritation.

Therefore, it was found to be suitable for use in skin cosmetic products.

Preparation of Sample: A portion of the sample was placed in sterile distilled water, boiled for 10 minutes, and extracted, and the extract was administered by Sampan in an amount of 0.5 g per 100 g of body weight.

CONCLUSIONS: The sample meets the criteria for no oral toxicity.

Experimental Example 5

In this Experimental Example, we tried to investigate the effects of purgatory powder, such as the present invention, on the effects of the growth of Digitalis plant cells. The experiment was commissioned to the Inha University Bioinformatics System Engineering Laboratory.

Below, the results of biomedical research experiments on the bioactivity of purgatory will be described in detail.

가 물이므로 물의변화와 인체에 대한 영향사이에는 많은 관련이 있으리라는 판단아래 경수를 대상으로 어떠한 영향이 있는지 알아보고 이어서 Digitalis 식물세포의 증식에 미치는 효과에 대한 실험을 수행하였다.In this experiment, 70 of the elements

Since water is water, we examined the effects of water on the water in the judgment that there would be many relations between the change of water and the effect on the human body.

end. Hard water change

[Experiment I]

100 ml of artificially-made hard water was transferred to each of the four flasks by 50 ml, and purgatory was placed on the bottom of two flasks for about 10 minutes.

To determine the change in hardness, a titration method using EDTA (titer 2.9412) was used. 1 ml of a buffer solution of ph10 was used, and EBT was used as an indicator.

The change in hardness was measured by the amount of EDTA until the moment when the color of the hard water was changed by EDTA. At this time, the color returned to its original state over time, but in this experiment, the measurement was made based on the changing moment.

[Result 1]

At 100 ppm of hard water, 1.70 ml of EDTA is required.

After the purgatory experiment, I need EDTA 1.25ml. Therefore, the hardness of 100.00ppm is lowered to 73.53ppm

연화되었음.That is, the hardness of 100.00ppm is 26.47

Softened.

[Experiment 2]

200ml of 100ppm hard water made by the same method as in Experiment 1 was transferred to a beaker to soak the purgatory. After about 30 minutes, the water in the beaker was divided into three flasks to measure the change in hardness. In this experiment, the hardness was measured by adding EDTA until the color of hard water did not change any more after the color of hard water changed by EDTA.

[Result 2]

Each 100ppm hard water before purgatory experiment

89.62 ppm

91.19ppm

89.62 ppm

경도 감소Average 90.14 ppm. Therefore 9.9

Hardness decrease

[Experiment 3]

This experiment was conducted with tap water. Tap water was placed in a container and transferred to six flasks, and purgatory was placed at the bottom of three flasks, and hardness was measured after about 5 minutes.

[Result 3]

Tap water without purgatory

97.48 ppm

97.48 ppm

97.48 ppm

Average 97.48 ppm

Tap water after purgatory experiment

91.19ppm

91.19ppm

91.19ppm

의 경도 감소Average 91.19 ppm. 6.5

Decrease in hardness

I. Effect of Purgatory on the Proliferation of Digitalis lanata Suspension Plant Cells

5 is a change in the total volume of the culture medium of the cells growing in the growth medium for 11 days. Since the depletion of the media components and the evaporation of the media occurs in proportion to the increase of the cells growing over time, the overall volume change of the culture medium reflects these factors. In particular, humidity and temperature in the atmosphere are closely related to evaporation of the medium, and such factors also affect the rate of cell growth. As shown in the figure, the use of purgatory shows that the volume of the total culture solution is slightly decreased compared to the control group which is not used. This is because the use of purgatory rather than the control has a faster cell growth rate against the evaporation of the medium or the depletion of nutrients, which may offset some of the rapid decrease of the depleted medium. This phenomenon is observed in the case where only the sunk cells were inoculated with purgatory cells continuously from day 4 to day 11 after inoculation, as shown in FIG. This fact can be estimated. Therefore, the use of nephrite showed a faster cell growth rate than the control without the nephrite. This result can be said to be the result of delaying the decrease of the total volume than the control by offsetting the decrease of the total culture volume due to nutrient depletion and medium evaporation. In addition, the result of comparing the volume of the increased cell alone is also observed to show a tendency to gradually increase the cell volume after 4 days, it is thought that can support this result.

Cell proliferation and dry weight are the most common measures of cell proliferation. In general, the growth curve of all cells is represented by the S-shaped curve, which can be classified into four stages. The period of adaptation to the medium, the lag phase in which there is no cell proliferation and no mass increase, and the exponential phase, which shows a curve with a steep slope due to the mass increase at the rapid proliferation rate. As the proliferation slows down, the stationary phase shows no mass increase, and the total cell volume or mass tends to rupture and die due to depletion of nutrients, discharge of toxic components, and saturation of cell density. It can be divided into decreasing dead phase.

7 and 8 show the results of measuring cell biomass and dry weight.

Figure 7 follows the four growth curves. In the case of using jade, after 7 days, the growth rate was slightly higher than that of the control without jade, and the growth stopped until 10 days, unlike the control showing the decrease in cell biomass. have. This is remarkable in that cell growth is continued even when the concentration of nutrient is almost depleted and the concentration in the medium becomes zero compared with the change in the concentration of glucose, a nutrient that acts as the main metabolic substrate in the medium. Do. It is common to show a tendency to stop the growth and decrease the growth rate at this point of time when nutrients are depleted, such as the control without the purgatory. However, when using the purgatory, it shows a different phenomenon. It is assumed to be.

This result is also shown in the figure measuring the dry weight of the cell in Fig. 8 shows the cell growth proliferating at a higher rate than the control after 3 days and the same phenomenon as in Fig. 7 from the 9th after.

As a result, cells grown close to purgatory in growth media showed a better effect on cell growth than controls that did not. Digitalis lanata plant cells, which have a growth limit of about 10 days, are maintained in a stable state without cell necrosis even after 10 days of incubation with nephrite without exchange with a new medium. Because of this phenomenon, it is expected to be applied as an advantageous method for optimizing cell growth and concentration in the production process for producing useful substances.

All. Effect of Purgatory on Changes in Medium pH

9 is a measure of the change in pH of the medium in the use of purgatory in the growth medium and in the control not used. The control group shows the tendency of pH in the medium to be applied to the general plant cells, which gradually decreases over time and is maintained at a certain level. However, by using the purgatory, it showed a similar pattern to the control group until the 7th day and increased gradually. This phenomenon was extremely shown in the result of FIG. 10 showing the result of the production medium in which the purgatory powder was added directly to the culture medium. . The control group without added jade powder showed a consistent drop in pH, while the addition of jade powder showed a tendency to maintain the value to some extent, which was due to the ionic reaction by minerals contained in the jade. It is guessed whether this acts as the cause.

la. Water quality assay results for purgatory

11 and 12 show the state and analysis results of the analytical measuring instrument. The data obtained therefrom are summarized in Table 10 as shown in Table 13 below.

Table 10 shows the analysis of each analysis item after 48 hours (Sample 1) by adding 20 g of nephrite mass in the solution. In this table, there is no change in each measurement item, and the effect of purgatory does not appear.

Table 11 shows the measured values for each analysis item after 48 hours (Sample 2) by placing 20g of jade nephrite on the outside of the solution. Table 13 also showed no change for each analysis item even when purgatory powder was placed outside the solution (sample 4). However, in the case of Sample 3, the data measured for each analysis item of the sample 48 hours elapsed after adding 20 g of purgatory powder in the solution is shown in Table 12. Same as

Cr, Pb, Ni, and Co, which are harmful to the human body, changed significantly after 48 hours.

Cr was removed in the absence of purgatory powder, that is, 45.30 mg before the reaction was removed to 0 after 48 hours in the purifying powder, and Pb was also completely removed from 13.76 mg to 0. Ni decreased from 51.8 mg to 1.733 mg and Co also decreased from 52.69 mg to 11.94 mg.

Mg, one of the essential elements in the human body, increased from 48.36mg to 55.74mg.

The hydrogen ion concentration of distilled water was acidic to pH 3.5, but after the reaction, it was changed to neutral to pH6.8 and the conductivity decreased rapidly. The adsorption force is physically large, about 3-4 mmol.q (equivalent) per gram.

이상의 증식을 보였다. 이는 고농도 배양을 위한 종래의 Digitalis lanata 식물세포에 대한 증식효과를 위해 수행했던 여러 가지 실험에서 조차 보기드문 좋은 증식결과이다.As described above, in the case of Digitalis lanata plant suspension cells cultured for one week with nephrite close, about 30 when there was no purgatory

It showed abnormal proliferation. This is a good result, which is rare even in the various experiments performed for the proliferation effect on conventional Digitalis lanata plant cells for high concentration culture.

In addition, experiments on how purgatory water affects hard water showed that purgium softened hard water without contact with water.

Particularly, in the component analysis experiments on water using distilled water, precipitation of nephrite was carried out. After 48 hours, in the component analysis, the pH was increased, the Ni and Co components were reduced, the Cr and Pb were removed, and the heavy metals were removed. An unusual phenomenon was observed that led to the phenomenon of.

이상 증식시킬뿐 아니라, Ni, Co성분의 감소, Cr, Pb의 제거등 인체에 유해한 중금속 성분을 제거 시킨다는 것은 상술한바 있는 실험예에서 나타나는 효능에 의해서도 확실한 것으로 생각된다. 또한 상기와 같은 실험결과는 본 발명의 연옥물질을 흡입하는 경우에 인체의 신진대사촉진 가능성 등을 시사해준다.As such, purgatory, nephrite, or gaseous purgatory materials have not yet been clearly identified theoretically, but they change the hardness of water without contact with water,

In addition to abnormal growth, it is believed that the removal of heavy metal components harmful to the human body such as reduction of Ni, Co components, removal of Cr, Pb, etc. is also confirmed by the efficacy shown in the above-described experimental example. In addition, the above experimental results suggest a possibility of promoting the metabolism of the human body when inhaling the purgatory material of the present invention.

Experimental Example 6

In this experiment, in order to find out whether the jade water with jade water such as jade material used in the present invention to the ultraviolet sterilization effect on the skin of the living body, experiments on the effect of jade jade powder and jade jade on the ultraviolet sterilization effect It was performed by the Department of Biology, Hanyang University.

The results of the research experiment are described in detail below.

1. Sample

Jade dust and agate collected from the Yeonok mine in Wolgok-ri, Dong-myeon, Chuncheon-gun, Gangwon-do, Korea.

2. Experimental strain and culture

Gram-positive bacteria, Bacillus subtilis, Gram-negative bacteria, Escherichia coli, and yeast Saccharomyces cerevisiae were selected as the control strains that can contact the skin most in nature. Strains of E. coli (gram negative) and Bacillus subtilis (gram positive) were cultured in nutrient agar medium (3 g yeast extract, 5 g bacto-peptone, 8 g sodium chloride / 1 liter of distilled water). After incubation at 37 ° C. for 24 hours, the colonies were counted. For Saccharomyces cerevisiae (Gerhardt et al., 1994), YPD (3 g yeast extract, 10 g bacto-peptone, 30 g dextrose / l liter of distilled water) was used. These strains were shaken for 24 hours at a temperature of 30 ° C. in a shaker.

3. UV irradiation method

1 ml of the strain cultured for 24 hours and diluted in sterile water, Bacillus subtilis, a Gram-positive bacteria and E. coli, a Gram-negative bacterium, were nutrient solid medium (3g yeast extract, 5g bacto-peptone, 8g sodium chloride, 15g agar) Saccharomyces cerevisiae, yeast Saccharomyces cerevisiae was inoculated with 0.1 ml each of YPD (3 g of yeast extract, 10 g of bacto-peptone, 30 g of dextrose, 15 g of agar) and 1 liter of distilled water. Each strain inoculated into a solid medium was placed under 50cm of ultraviolet light (115V, 30W, 2537Å tube, etc.) installed in a sterile zone (hand-made line) for each strain. Gram-positive and gram-negative bacteria were irradiated with UV light for 40 seconds and 1 minute, yeast for 30 seconds and 1 minute, and UV light in the sterile zone was measured with a photometer (Licon LI-189). As a control, the number of cells in a solid medium not irradiated with ultraviolet light was used.

4. Distribution of hemolytic bacteria

무균 탈섬유의 양피(sheep blood)를 첨가하여 사용하였다. 옥분과 옥수를 혈액한천배지에 도말하여 37℃ 배양기에서 24시간 동안 배양하였다. 배양한 평판을 꺼내 집락의 형태와 용혈의 특징을 관찰하였으며, 집락 주위가 녹색환이 형성되면 α-용혈로, 집락 주위가 완전히 투명하게 녹은 용혈환이 나타나면 β-용혈로 판정하였다. 또한 용혈성이 전혀 없는 경우는 비용혈(γ형)로 판정하였다.Blood agar plates (BAP) were used to count hemolytic bacterial populations known as pathogenic bacteria in jade and corn (Benson, 1994). Blood agar medium (Trypticase soy agar (BBL) 40g / 1 liter of distilled water) is sterilized after cooling to 48-50 ℃ 5

Sterile defiber sheep blood was added and used. Jade powder and cornmeal were plated on blood agar medium and incubated for 24 hours in a 37 ℃ incubator. The cultured plates were taken out, and the colony morphology and hemolysis characteristics were observed. If a green ring was formed around the colony, it was determined as α-hemolysis. In addition, when there was no hemolysis at all, it was determined as nasal blood (type γ).

5. Measurement of Microbial Communities

end. Microbial community according to the concentration of jade and chalcedony

, 25

, 50

, 100

(v/v)의 농도구배로, 옥분말은 5

, 10

, 20

, 30

(w/v)의 농도구배로 혼합하여 그람양성 세균과 그람음성 세균은 37℃에서 24시간, 효모는 30℃에서 24시간 배양하여 계수하였다. 대조군으로는 옥수와 옥분말을 첨가하지 않은 액체배지를 사용하였다.To investigate the effects of jade and agate on the growth of gram-positive bacteria, gram-negative bacteria, and yeast, nutrient and medium were grown in a constant concentration in nutrient liquid medium and YPD liquid medium. Agate 10

, 25

, 50

, 100

concentration gradient of (v / v), jade powder is 5

, 10

, 20

, 30

Gram-positive bacteria and Gram-negative bacteria were cultured at 37 ° C. for 24 hours and yeasts were cultured at 30 ° C. for 24 hours. As a control, a liquid medium without corn and jade powder was used.

I. UV protection effect

UV light was irradiated over time to calculate the number of viable cells of gram-positive bacteria, gram-negative bacteria, and yeast in terms of the percentage of UV-blocking effect. Gram-positive bacteria and Gram-negative bacteria were plated on three nutrient solid media and irradiated with ultraviolet rays (115V, 30W, 2537Å tube, etc.) and incubated at 37 ° C for 24 hours. Yeast was counted by smearing on YPD and incubating at 30 ° C for 24 hours. The cell count was determined by colony forming unit (CFU) / ml.

[result]

Hemolytic inoculation of bacteria on the blood agar medium, depending on the type of hemolysis ring formed around the colony, is divided into α-type, β-type. In addition, when there is no hemolysis at all, it is called nasal blood ((gamma) -type). α hemolysis is the change of oxyhemoglobin to metemoglobin (methemoglobin), the green ring is formed around the colony, β hemolysis is the hemolysis ring is completely transparent around the colony. This hemolysis is caused by hemolytic toxins. Hemolytic colonies have been identified in jade and chalcedony. Β-hemolysis in jade powder was 930 CFU / ml, γ-hemolysis was 190 CFU / ml, β-hemolysis was 30 CFU / ml and γ-hemolysis was 730 CFU / ml. The result of detecting more β-hemolytic bacteria in jade powder than in chalcedony was because it was collected in the hot powder of jade powder.

2. Measurement of Microbial Communities

, 25

, 50

, 100

의 농도구배로 혼합하였다. 옥분말은 5

, 10

, 20

, 30

의 농도구배로 혼합한 후 배양하여 옥분과 옥수를 첨가하지 않은 대조군과 비교하였다. 옥수에서는 옥수를 전혀 첨가하지 않은 대조군과 비교할 때 Bacillus subtilis와 Saccharomyces cerevisiae에서는 거의 비슷한 양상을 보였으나, E. coli는 10

, 25

, 50

의 옥수 첨가시 대조군보다 많은 개체수를 보였다. 이는 옥수에 함유된 미량원소가 E. coli의 생장에 영향을 주었고 Bacillus subtisis와 Saccharomyces cereviliae 에는 영향이 없었던 것으로 판단된다. 따라서 옥수에 함유된 미량원소의 성분과 함량을 규명해야만 미생물의 성장과 연계한 해석이 가능할 것이다. 옥분에서는 Bacillus subtilis, E. coli, Saccharomyces cerevisiae 모두 대조군보다 적은 개체수를 보였다. 또한 옥분말의 농도가 높을수록 개체수가 감소하는 경향이 나타났다.10 Acorns in nutrient medium and YPD medium

, 25

, 50

, 100

Mix with a concentration gradient of. Jade powder is 5

, 10

, 20

, 30

After mixing with the concentration gradient of the culture was compared with the control group without addition of jade and corn. In chalcedony, Bacillus subtilis and Saccharomyces cerevisiae showed almost similar results compared to the control group without chalcedony, but E. coli showed

, 25

, 50

Addition of corn showed more population than control. It is believed that the trace elements contained in corn affected the growth of E. coli and that Bacillus subtisis and Saccharomyces cereviliae were not affected. Therefore, it is possible to analyze the microorganism growth in connection with the component and content of trace elements contained in corn. In jade powder, Bacillus subtilis, E. coli and Saccharomyces cerevisiae were all smaller than control. In addition, the higher the concentration of jade powder, the tendency of decrease was shown.

3. UV protection effect

The microorganisms of the normal bacterial flora can be distinguished from resident bacteria and old bacteria. Although resident bacteria remain somewhat persistent, there are a number of factors that affect permanence and its extent. In other words, depending on the climate temperature and humidity can be increased, which can increase the density of skin microorganisms. Children have more Gram-negative and latent pathogens than adults. Therefore, digestive organisms such as Gram-positive bacteria such as Bacillus subtilis and E. coli are continuously inoculated on the surface of the skin.

, 30

의 농도로 첨가한 액체배지에서 각각 23

, 13

로 옥분말을 첨가하지 않은 대조군의 12

보다 생존 개체수가 많았다. 또한 옥수를 첨가한 액체배지에서도 비슷한 경향을 보였다. E. coli의 경우 가장 높은 생존 개체수를 보였으며, 옥수첨가 액체배지에서보다도 옥분첨가 액체배지에서 이러한 양상은 두드러졌다.UV protection effect of jade powder and corn was confirmed by UV sensitivity of microorganisms. Bacillus subtilis, a Gram-positive bacterium, produces 20 powders of jade when irradiated with UV light for 40 seconds.

, 30

In liquid medium added at a concentration of 23

, 13

12 of the control group without jade powder

There were more survivors. Similar trends were also observed in the liquid medium with the addition of corn. E. coli had the highest viable population, and this pattern was more pronounced in jade added liquid medium than in chalcedony medium.

, 옥분말 첨가 액체배지에서는 82

로 나타났으며, 옥분 10

의 농도 액체배지에서는 90

의 가장 높은 생존 개체수를 보였다. 미생물 종류에 상관없이 자외선 차단효과는 옥수보다 옥분말에서 높게 나타났다.That is, on average, 74

In liquid medium containing jade powder, 82

Appeared to be 10 minutes

In liquid media of 90

Had the highest survival population. Regardless of the type of microorganism, UV blocking effect was higher in jade powder than in corn.

UV sensitivity of microorganisms is known in the order of Gram-negative bacteria, yeast, spore-forming bacteria, fungi, and is affected by the biological activity, bacteria, moisture, environmental substances, etc. In addition, the photoreaction after ultraviolet irradiation is different depending on the species. Ultraviolet rays have the maximum bactericidal capacity at a wavelength of 260 nm, and E. coli and typhoid fever have been reported to die within 10 minutes when a 15-watt UV lamp is irradiated at a distance of 50 cm [芝 騎 勳, 1983]. In the case of Cryptococcus neoformans, changes in the contents of the cells began to occur as well as the morphological changes of the cells themselves after 10 minutes of treatment. UV sensitivity was confirmed in the order of Bacillus subtilis, Saccharomyces cerevisiae, Escherichia coli. In other words, the UV blocking effect was confirmed in the order of E. coli, Saccharomyces cerevisiae, Bacillus subtilis. However, when considered in conjunction with the results of the evaluation of the growth effects of oak and agate, E. coli, which has a relatively large population, showed the highest blocking effect. In addition, the bactericidal effect of sunlight on E. coli and Proteus vulgaris in the intestinal bacteria was bactericidal in proportion to the average insolation, but this bactericidal effect was related to the result reported by the action of visible light of short wavelength, not ultraviolet light. Therefore, the study of the bactericidal effect according to sunlight should be supplemented.

[conclusion]

1. The average hemolysis of jade was 930CFU / ml, the γ hemolysis was 190CFU / ml, the β-hemolysis was 30 CFU / ml and the γ hemolysis was 730 CFU / ml.

2. The size of microbial populations grown in jade powder and corn supplemented medium showed a change according to the concentration of jade powder rather than the concentration of chalcedony.

3. As a result of the measurement of the number of microorganisms surviving after UV irradiation, UV-blocking effect was higher in jade powdered liquid medium than in jade-added liquid medium.

4. The sunscreen effect was confirmed in the order of E. coli, Saccharomyces cerevisiae, Bacillus subtilis.

As described above, the purgatory material used in the product of the present invention has been found to have a very high UV blocking effect.

As described above, when the product of the present invention is used, as a purifying substance contained in oxygen as well as supplying oxygen, medical efficacy, for example, promotes the metabolism of the human body, and can treat diseases (headaches, hand and feet, indigestion and insomnia, etc.). Has an excellent effect.

Claims (2)

A breathing can, comprising: a can body; And a gaseous purgatory material containing a mineral substance filled with a predetermined pressure, such as oxygen, inside the can body at a predetermined pressure, and exhausted at a pressure that can be sucked when the can body is opened. The meteorological purgatory and oxygen are

Portable vapor purifying cans, characterized in that the composition as ().

The suction device of claim 1, wherein the suction device is detachably coupled to the can body, and provides an inhalation path through which a purifying substance of gaseous phase discharged when the can body is opened through the human respiratory tract. The suction device further includes a bellows-type cap coupled to surround one side of the can body, a suction hose formed integrally with the bellows-type cap to form a suction path communicating with the outside of the can body; A portable gaseous purgatory breathing can, comprising: a mask-type suction port installed in contact with one end of the suction hose.

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