KR100257995B1 - A capsule and tablet of soft jade and preparation thereof - Google Patents

Abstract

PURPOSE: A method for preparing a capsule and a peel by using nephrite is provided for obtaining excellent medical effects by crushing a lump of nephrite and filtering the crushed nephrite, and capsuling the nephrite powder. CONSTITUTION: Drinkable or edible nephrite products contains 30wt.% of the nephrite powder having 400-800mesh of particle diameter to form nephrite capsules. The nephrite powder 200mg is filled in a capsule at the room temperature 5deg.C and the speed 400-600CAP/min with the humidity 10%. A gelatin solution for making a ball-type capsule contains 30wt.% of nephrite powder, 25wt.% of gelatin and 45wt.% of purified water.

Description

Capsules and tablets prepared by using purgatory

The present invention relates to a method of manufacturing a capsule using a purgatory, in particular, from the purgatory of the fine-grained collagen fiber brown rice structure of the hollow-glazed-Yangseok-based fibers are very fine. The Nephrite Jade obtained is manufactured in capsule form so that it can be taken at all times, and is used to promote metabolism of the human body such as prevention of diseases of the human body (depression, dizziness, headache, etc.), impurities removal function (removal of heavy metals, etc.). The present invention relates to a method for producing a capsule using a purge which is applicable to a wide range of applications.

Jade is generally divided into jadeite and jadeite. In addition, jadeite is a mineral in the lead fluoride group belonging to the pyroxene family, which is a monoclinic substance composed of silicic acid, aluminum oxide, and soda, and its hardness is similar to that of crystal. Dense lumps, light black, cyan, green, transparent or translucent, usually jade refers to this jadeite.

Purgatory is an inosilicates monolithic whiskey mineral, which is divided into purgatory and serpentine superbasic purgatory among high-quality surrogate rocks, and their quality is defined by their fine structure, It is known that the hollowed-out scallop-Yang Ki-Sperm is made of fast and fiber, and the thinner it is, the better the quality (Mining Promotion 1993 New Year's Issue: Korea Mining Promotion Corporation).

On the other hand, according to a report published in the German medical literature (“KRAFT der KRISTALLE und der EDELSTEINE” by Mauda Palmer Die Verborgene), jadeite and jade are two different ores, which, like most gemstones, contain silicon and oxygen, but jadeite Compared to being formed of silver granular crystals, purgatory stones are composed of countless crystals such as fibers and hair and fine aggregates. Especially, purgatory stones are three minerals that are beneficial to the human body, unlike sodium and aluminum in jadeite. That is, it has been reported recently that calcium, iron, magnesium, etc. as the main component has a great effect on the healing of illness caused by high blood pressure, diabetic circulation disorder, heart disease and kidney disorders when worn purgatory.

Also, in Dongbobogam, a classic of oriental medicine, purgatory is put in Omiju and Jiyuju, and it is good to eat when you put it in water of Hakjangsu. When eaten in the same size as 麻豆 大, the waste is discharged to the outside of the body, 1 oxol, 1 jijicho (地 楡 草), 1 bream (滔 米), 3 egret (白露) mixed together and put in a copper bowl When the rice is cooked, the oxol turns into water, which is called oxal (玉液) and is called Shinseonokjang (신농 옥장). Shinnongboncho, Dangboncho, and the main vines (本草綱目) According to them, taking jade as powder to make it enlightening will not only have the effect of moisturizing the five intestines and completely discharging waste products in the body, but also removing the heat in the stomach, which is effective for the digestive system. Anemia with bronchial asthma, fever and heartburn Good, thirst-quenching, sesame-like blouse makes the body lighter and longer to fly, rejuvenates the functioning of the lungs, helps the vocal cords to vocalize, is good for the throat, nourish the hair, and functions of the intestines It is effective in alleviating neurological disorders during stress.In addition, it is good to eat white jade powder when the muscles are tense and rats get sick, and rubbing the wounds with purgatory for several days when there are scars on the face and body Purifying the main components of purgatory has been known to exert an excellent effect on the human body with little side effects.

However, purgatory has been limited to its use as some jewelry for jewelry, such as necklaces, rings, and wrists, despite its scarcity.

In addition, the processing of nephrite requires a lot of time, effort, and careful attention, and it is impossible to make it unless it is a skilled person with a long experience, and since it is expensive, it is economically disadvantageous to develop the use of nephrite generally. This situation was absent, and therefore, its research and development is in dire need.

Applicant paying attention to the excellent medical effect of the purgatory as described above, the Applicant, "method of adding nephrite powder during the manufacturing process of the soft drink (Patent No. 118,114)" and "method of adding the purgatory powder during the manufacturing process of the skin patch; and The product (Patent No. 129,551) and "Glass products containing jade powder and its manufacturing method (Patent No. 112812)" and many others have been patented at home and abroad.

Therefore, the object of the present invention was developed in the light of the above-mentioned research results, the fine crushing of the nephrite (塊狀) crushed finely obtained by encapsulating the purgatory powder obtained by filtering it, taking the excellent medical efficacy of purgatory It is to provide a method for producing a capsule using a purgatory.

A feature of the present invention for achieving the above object is a method of producing a drinkable or ingestible product using purgatory according to the invention of claim 2, wherein the total weight ratio of the known capsule forming process is acceptable. The nephrite capsule was formed by adding 30 wt% of the nephrite powder powdered to a particle size of 400-800Mesh, and in the nephrite capsule, the nephrite powder powdered to a particle size of 400-800Mesh was subjected to an indoor temperature of 5 ° C and an indoor humidity of 10%. It is characterized in that it is supplied to the capsule charger in the state to charge 200mg at a rate of approximately 400-600CaP / min.

The particle size, that is, the particle size of the jade powder is generally 100-350 mesh, but when ductility is to be increased, it is advantageous to use the finer particles of 350-1000 mesh, which are finer particles. If the grain size of the purgatory powder is larger than the above range, the roughness of the product is increased, which is not preferable.

In addition, when the addition amount of the jadeite powder is less than 10% by weight, it is not preferable because the effect due to the addition of jadeite is not preferable. In particular, there is a risk of weakening the product by reducing the ductility, as well as the product price is increased due to the high price of nephrite powder is less than 30% by weight of the total weight of the capsule raw material is appropriate.

1 is a graph showing the results of observing the change in the total volume of the culture medium of digitalis lantana (Digitalis lanata) cells growing in the growth medium (Experimental Example 5),

2 is a graph showing the change in the volume of digitalis lantana (Digitalis lanata) (Experimental Example 5),

3 is a graph (Experimental Example 5) showing the change in the bio weight of the cell,

4 is a graph showing the change in dry weight of cells (Experimental Example 5),

5 is a graph (Example 5) showing the pH change of the digitalis lantana (Digitalis lanata) culture medium,

6 is a graph showing the pH change of the production medium after the addition of nephrite powder (Experimental Example 5),

7 is data outputting the condition conditions of the analyzer (Experimental Example 5),

8 is a data output of the analysis results (Experimental Example 5).

Hereinafter, a more preferred embodiment of the capsule manufacturing method using the purgatory according to the present invention will be described in detail.

<Preparation Example of Purgatory Capsule>

[Manufacture of purgatory powder]

First, the nephrite gemstones mined from the nephrite ore are put in a crusher (not shown) and finely crushed. This finely crushed jade is again screened, that is, it is transferred to a filter and continuously filtered to obtain a fine powder of jade powder that does not give any resistance when taken. The size of the mesh of the screen to obtain the purgatory powder is preferably about 200-800Mesh. Purified powder obtained by the above method is used as a filling material and capsules for preparing empty capsules such as gelatin and purified water.

Capsule Preparation

The manufacturing method of the empty capsule is as follows.

The gelatin solution containing the nephrite powder is mixed by mixing 25 wt% gelatin and 45 wt% purified water with respect to 30 wt% of the prepared nephrite powder.

That is, after adding nephrite (30 wt%) to gelatin (25 wt%), 45 wt% of purified water is poured therein, and left to stand for 24 hours. This is then heated to form a solution. In the composition of the gelatin solution, a trace amount of pigment (coloring agent) or sweet syrup that can give various colors can be added.

As a gelatin solution containing the jade powder prepared in this way, a stainless capsule-molding mold (Mjold) (not shown) is deposited. The encapsulating mold is pre-cooled and soaked in the gelatin solution. When the encapsulated mold is immersed in the gelatine solution and taken out, the gelatine solution adheres to the surface of the mold as a thin film. When the capsule molding mold is cooled again in this state, the gelatin membrane containing the purgatory powder adhering to the surface of the mold solidifies into a film of uniform thickness. When this is dried and cut under certain conditions, it is possible to obtain a predetermined purgatory capsule, that is, a purgatory capsule consisting of a cap and a capsule body.

The thickness of the nephrite capsule can be arbitrarily adjusted by the viscosity of the gelatin solution to which the nephrite powder is added and the deposition speed of the capsule molding mold, and the size of the capsule can also be variously manufactured according to the size of the capsule molding mold. In addition, the nephrite capsule can be produced as a soft or hard capsule according to the control of the additive.

The nephrite capsule body prepared as described above is filled with nephrite powder powdered at a particle size of 200-800Mesh.

Purgatory powder filling into the purgatory capsule is carried out in a dry atmosphere as possible to modify the purgatory capsule and the purgatory powder. It is desirable to maintain a room temperature of 5 ° C. and a room humidity of 10%. In addition, the filling operation of the purgatory powder is preferably carried out by a capsule charger not shown. The capsule charger (not shown) is preferably used to fill the purgatory capsule by about 200mg at a rate of about 400-600CAP / min, the filling rate and filling capacity of the charger can be arbitrarily modified according to the size of the capsule. In addition, it is preferable to fill the purgatory capsule in the purgatory capsule with a filling amount of approximately 3/4.

Capsules filled with nephrite powder are packaged as a predetermined unit to be sold after the cap is sealed. In addition, the surface of the capsule may be coated with a bowel solution to dissolve in the intestine (腸). Since this method is a general technique, a detailed description thereof will be omitted.

<Production example of purgatory tablet>

First, the preparation of the nephrite powder is the same as the preparation of the nephrite powder in the nephrite capsule, and a separate description thereof is omitted.

The mixture is formed by uniformly mixing the additives with respect to the prepared jade powder. The composition ratio of the nephrite powder and the additive is preferably 25 wt% binder, 15 wt% disintegrant and 5 wt% lubricant as an additive with respect to 60 wt% of the nephrite powder.

Binders used above are materials that are used to make molding easier by giving binding force to purifying powder powder, which is a powder raw material of tablets, and using water as a solvent, dissolving in an organic solvent, and alone as a binder. Acting water, acetone, and the like, and in the present preparation, it is preferable to use purifying water as a binder.

Disintegrators used above are added during the manufacture of tablets to promote disintegration of the tablets, and starch and calcium salts of CMC (CML-ca) are generally used. The disintegrating method can be prepared by first adding the disintegrant to the purgatory powder raw material at the same time as in the above preparation, and at first, a part of the disintegrating agent is added to the nephrite powder raw material, and the remaining disintegration during tableting. You can also add an agent.

The lubricants used above are added to facilitate the compression operation of tablets, and corn starch, talc, magnesium stearate, calcium stearate, and the like are generally used, and these are used rather than independently. It is preferable to use a lubricant made by mixing two substances such as magnesium stearate and talc.

As described above, the mixture made by mixing the additive with the raw material of the purgatory powder is put into a refiner (not shown) to make a tablet. There are many types of the refiner, the following purifier (not shown) was used to prepare the purgatory powder.

That is, a tablet machine with a production capacity of 10,000 (tabs / min) was used to produce a tablet having a diameter of about 13 mm with a pressure of 80 kN. Purified tablets (not shown) are the internal tablets of Plain Tablets, which are taken as hot or cold water with anticipated systemic effects. If necessary, the tablets of enteric solution are coated on the surface of the tablets. It can also be made with enteric-coated tablets or coated with sugar-coated tablets to sugar-coated tablets. Purgatory tablets are packaged and shipped in a unit that is convenient to sell. In the above-described production example, the tablet is manufactured to have a diameter of about 13 mm, which is described as an example. However, the size and shape (original or lens shape) of the tablet may be arbitrarily modified.

Purified capsules and tablets were randomly selected in adult men and women, especially those with disease. As a result, all purgatory substances were purified by impurities. It can be seen that there is a medical effect to strengthen the function of the body.

Such medical efficacy can be seen from the following examples of experiments performed using the product and such purgatory materials.

Experimental Example 1

In this experimental example, two or three tablets of purgatory capsules or purgatory tablets of the present invention were administered to 50 adult men and women who complained of symptoms such as headache, insomnia, numbness and dyspepsia at least three times a day. The dose was taken for 30 days, and the effect was evaluated by a questionnaire. The results are shown in Table 1 below (Department of Oriental Hospital of Daejeon University).

As shown in the experimental results, when taking the product according to the present invention, it can be seen that the headache (80%), insomnia (81%), low hands and feet (90%), indigestion (89%) is significantly alleviated. .

Experimental Example 2

In this experiment example, the experiment was carried out to the Korea Food Research Institute to investigate the effect on the breeding and growth rate of rats when treated with the nephrite used to prepare the present invention and the nephrite water in which the nephrite powder was precipitated. It carried out by request. Details are as follows.

Nine-week-old Sprague Dawley rats were prepared for 30 males and females. The rats of group A were allowed to drink tap water in cages fed with meteorological purgatory, and the rats of group B were allowed to separate and drink the supernatant after dipping purgatory powder (2 mg / ml tap water). We did not put in the material that was injected. In the C group, the control group was free of air or chalcedony containing jadeite. All treatment groups were seeded for 9 days and then males were isolated and slaughtered for sperm concentration and vitality testing. The results are shown in the table below.

As a result of the experiment, purgatory results in the A group and the B B group treated with purgatory used in the present invention were superior to those of the control group. The delivery rate was 90% in the A and B treatment groups and 80% in the C treatment group, and the number of male pups was 3 in the A and B treatment groups, although there were 6-8 more in the control group. There were more horses. The days to postpartum delivery were significantly shorter in the A treatment group. In addition, sperm concentration and vitality of sperm were excellent in group A treated in cages fed with jade. However, the effects of simultaneous treatment with purgatory powder and agate were not studied in this experiment.

In conclusion, the above results suggest that nephrite and nephrite affect the reproduction and growth of rats, in which the nephrite inhalation or agate treated group showed better reproduction and growth index. It is expected that a lot of valuable research will be performed on these phenomena. However, these results are considered to be valuable as basic data for improving reproductive efficiency and enhancing sperm concentration and motility.

Experimental Example 3

This experiment was performed by the Korea Food Research Institute to investigate the effect of various purgatory on the symptom relief of the streptozotocin-induced diabetes in rats. Details are as follows.

Diabetes was induced by intraperitoneal administration of St (streptozotocin) (35-40 mg / kg body weight dissolved in 0.1 M citric acid in pH 4.0) to 32 Sprague Dawley rats weighing 252-292 g.

After 7 days of ST administration, using only europaper (Eiken Chemical Co., Ltd., Japan), only urine glucose of more than 250mg / rats were selected and placed in 5 groups per treatment group for 50 days. Animals of group A were intraperitoneally injected with 1.0ml of injectable purifying distillate (pH 6.40) in group B. Group B was reared in cages containing jade material. The solution was fed daily as a negative water, and the group D was administered only ST as a control group, the experimental feed was supplied with commercial feed, and the breeding method was in accordance with general practice. Blood was collected from the abdominal aorta, and the blood was left at room temperature for 30 minutes and then centrifuged at 5,000 rpm for 15 minutes to obtain plasma.

The weights of organs such as liver and kidneys were weighed and recorded. Plasma β-hydroxybutyrate (HBA), free fatty acids (FFA), cholesterol, HDL-cholesterol and triglycerides were measured. The HBS content was quantified by measuring the increase in absorbance (OD) at 340 nm of NADH produced by the oxidation reaction by β-hydroxybutyrate dehydrogenase, and the blood lipids were measured in the clinical trial kit (Eiken Chemical Co., Ltd.). , Japan).

p <0.05

^a β-hydroxybutyrate (ketone body)

^b free fatty acids

^c total cholesterol

^d Eutriglycerides (neutral lipids)

^{e high} density lipoprotein cholesterol

Body weight increased in the C group fed the nephrite corn, while it was decreased (B, D) or maintained in the other group (A). In diabetic rats, renal hypertrophy is known to be a common symptom. Similar trends were observed in this study, and kidney weight in group C was the lowest among the treatment groups except the control group. Hepatomegaly was also seen due to diabetes, but liver weight was the lowest in rats fed corn. Blood glucose, ketone body and FFA concentrations were highest in the A, B, C, D treatment group. Plasma cholesterol and triglyceride concentrations showed the same trend (A> B> C> D). From the results, rats fed the nephrite (C group) showed relatively lower values of all the indexes than the other two experimental groups, and blood cholesterol and FFA concentrations were lower than those of the control group without the nephrite treatment. It was.

Blood cholesterol (TC) and neutral lipid (TG) concentrations were generally higher in the order of A, B, C, D treatment groups. When the glycemic index was determined by the post-prandial blood glucose response, the experimental results may vary according to the investigator. However, in the present experimental example, a commercially available complete formula (TMR) without considering the glycemic index of cereals in the experimental feed was considered. Even though they were fed, the treatment group (Group C) who was fed corn was improved hyperlipidemia, one of the symptoms of diabetes. HDL-cholesterol content did not differ between treatment groups.

In conclusion, when feeding meteoric nephrite and nephrite water to streptozotocin-induced diabetic rats, not only prevented weight loss, but also prevented hypertrophy of kidney or liver and improved hyperlipidemia, a type of diabetes mellitus.

Experimental Example 4

In this experimental example, an experiment was conducted on the effect of the purgatory powder used in the preparation of the present invention on the experimental sphere (rabbit) (US FDA-approved laboratory).

Method: US Federal Register Vol 43.No 163-1978.8.22 p37359

Experimental procedure: Place 0.1ml of extract on the inverted lower eyelid of one eye, and then gently hold for 1 second before placing the upper and lower eyelids. Observe the other eye without treatment.

The treated eyes of six rabbits were left unwashed, and the eyes of three rabbits were washed with lukewarm water for one minute immediately after treatment. Prior to treatment with the sample, the eyes were examined in advance by fluorescence and only animals that did not show corneal damage were used for the test. Eye lesions were observed 24 hours, 48 hours, 72 hours, 7 days and 14 days after treatment.

result:

I. cornea

(A) Opacity-Density (Area for Detox)

Normal-0

Diffuse Areas Scattered—Details of the Iris are Visible-1

Translucent areas that are easily identified, the details of the iris are slightly blurred-2

Milky white area, details of iris are not visible, pupil size is almost indistinguishable-3

Opaque, no iris visible-4

(B) corneal area

Normal-0

Less than 1/4, but not 0 -1

More than 1/4, less than 1/2 -2

1/2 or more, 3/4 or less -3

More than 3/4, less than total area -4

Score is A × B × 5, total maximum = 80

Ⅱ Iris

(A) value

Normal-0

Folded above normal, hyperemia, swelling, pericorneal bleeding (these symptoms appear in any size, alone or in combination), the iris still responds to light (a slow response is apparent) -1

Not responding to light, bleeding, total destruction (one or all of these symptoms) -2

Score is A × 5, maximum possible total = 10

III. conjunctiva

(A) Red tide (only related to the conjunctiva of the eyelid)

Normal -0

Blood vessels are definitely hyperemia over normal -1

More diffuse, deeper magenta, individual vessels not easily distinguished -2

Spread red like beef -2

(B) chemosis

No pour -0

Random swelling phenomenon -1 above (including the blinking film)

Obviously swollen with partial inversion of the eyelids -2

Eyelid half closed and swollen -3

Eyelid half to full closed and swollen -4

(C) secretion

Normal -0

Any phenomenon other than normal (does not include small amounts observed at the inner edge of normal animals) -1

Secretion wet only on the eyelids and the eyebrows adjacent to the eyelids -2

Occupies a significant area around the eyes -3

Score (A + B + C) × 2, total max. = 20

CONCLUSION: This sample has been determined to be free of eye irritation and therefore suitable for use in eye cosmetic products.

Method: US Federal Register Vol. 43, No. 163

CTFA (TheCosmetic, Toiletry and Fragrance Association, Washignton, D.C.)

Experimental procedure: Patch testing was performed on the skin of the white rabbit and the original skin. The hairs are shorted off at the back and sides. Two areas, about 10 cm apart, were selected as patch locations. One area is made of four small epidermal incisions in the patch area to exfoliate the skin (two of which are perpendicular to the other, using a “tic-tac-toe” method). Patches of 1 inch each of the surgical gauze are attached using a thin rubber band and adhesive tape. Samples are introduced at each site in an amount of 0.5 ml (g) under the patty. The body of the animal is wrapped with a rubberized cloth for 24 hours of exposure time. The animal is immobile during the exposure time. After removing the patch, the reactions of each site were evaluated based on the scores described below.

Evaluation of Skin Response

Thorax and scalp formation

No nevus -0

Very weak thorax (recognizable) -1

Properly Identified Thoracic-2

Moderate to severe thorax-3

Severe thorax (beat red) to slight crust formation (deep damage) -4

Edema

No edema-0

Very weak edema (recognizable enough) -1

Moderate edema (approximately 1 mm rise) -3

result :

Preparation of Sample: A portion of the sample was placed in sterile distilled water, boiled for 10 minutes, and extracted, and the extract was administered by intubation in an amount of 0.5 g per 100 g of body weight.

CONCLUSIONS: The sample meets the criteria for no oral toxicity.

Experimental Example 5

In this experiment example, to find out whether or not the purgatory powder used in the preparation of the present invention has a good effect on the activity of plant cells as another method to determine the various effects on the living body of the digitalis (Dgitalis) plant cells The experiment on the effect on proliferation was performed by Inha University bio information systems engineering laboratory.

In the following, the results of biomedical research experiments on the bioactivity of purgatory will be described in detail.

In this experiment, 70% of the constituent elements of the human body are water. Therefore, the impact of water on the water and the impact on the human body are investigated. Experiments on the effects were performed.

end. Hard water change

Experiment 1

100 ml of artificially hard water was transferred to each of the four flasks in 50 ml, and then purgatory was placed on the bottom of the two flasks for about 10 minutes.

To determine the change in hardness, a titration method using ethylene diamine acetic acid (EDTA; titer 2.9412) was used. 1 ml of a buffer solution of ph10 was used, and EBT was used as an indicator.

The change in hardness was measured by the amount of ethylene diamine acetic acid until the color of hard water changed by ethylene diamine acetate. At this time, the color returned to its original state over time, but in this experiment, the measurement was made based on the changing moment.

Result 1

At 100 ppm of hard water, 1.70 ml of ethylene diamine acetate.

1.25 ml of ethylene diamine acetic acid after the purgatory test. Therefore, the hardness of 100.00ppm is reduced to 75.53ppm

That is, the hardness of 100.00 ppm was softened 26.47%.

Experiment 2

200ml of 100ppm hard water made by the same method as in Experiment 1 was transferred to a beaker to soak the purgatory. After about 30 minutes, the water in the beaker was divided into three flasks to measure the change in hardness. In this experiment, the hardness was measured by adding ethylene diamine acetic acid until the color of hard water changed no more after changing the color of hard water by ethylene diamine acetate.

Result 2

Each 100ppm hard water before purgatory experiment

89.62 ppm

91.19pm

89.62 ppm

Average 90.14 ppm. 9.9% hardness reduction

Experiment 3

The experiment was conducted with regular tap water. Tap water was placed in a container and transferred to six flasks, and purgatory was placed at the bottom of three flasks, and hardness was measured after about 5 minutes.

Result 3

Tap water without purgatory

97.48 ppm

97.48 ppm

97.48 ppm

Average 07.48pm

Tap water after purgatory experiment

91.19ppm

91.19ppm

91.19ppm

Average 91.19 ppm. Thus 6.5% hardness reduction

I. Effect of Purgatory on the Proliferation of Suspension Plants of Digialis lanata

Figure 1 shows the change in the total volume of the culture medium of the cells proliferating in the growth medium for 11 days. Since the depletion of the media components and the evaporation of the media occurs in proportion to the increase of the air bubbles that grow over time, the volume change of the overall culture medium reflects these factors. In particular, humidity and temperature in the atmosphere are closely related to evaporation of the medium, and such factors also affect the rate of cell growth. As shown in the figure, the use of purgatory shows that the volume of the total culture solution is slightly reduced compared to the unused control. This is because the use of purgatory rather than the control has a faster cell growth rate against the evaporation of the medium or the depletion of nutrients, which may offset some of the rapid decrease of the depleted medium.

As shown in FIG. 2, which measured the volume change of only the cells that subsided this phenomenon, the use of purgatory from 4 to 11 days after inoculation of the cells showed more increase in cell volume than the control group which was not used. This fact can be estimated. Therefore, the use of nephrite showed a faster cell growth rate than the control without the nephrite. These results can be said to be the result of delaying the decrease of the total volume than the control by offsetting the decrease in the total culture volume due to depletion and evaporation of the medium. In addition, the result of comparing the volume of the increased cell alone is also observed to show a tendency to gradually increase the cell volume after 4 days, it is thought that can support this result.

Cell proliferation and dry weight are the most common measures of cell proliferation. In general, the growth curve of all cells is represented by the S-shaped curve, which can be classified into four stages. Lag phase, which is a period of adaptation to the medium, in which there is no proliferation of cells and in which the mass does not increase, and an exponential curve showing a steep slope as the mass increases at a rapid proliferation rate. phase), the growth is maximal to some extent, the growth is slowed down, the stationary phase shows no mass increase, and the cells are ruptured due to the depletion of nutrients, the release of toxic components, and the saturation of cell density. The tendency to die can be divided into a dead phase where the total cell volume or mass is reduced.

3 and 4 show the results of measuring cell biomass and dry weight.

Figure 3 follows the four growth curves. In the case of using jade, after 7 days, the growth rate was slightly higher than that of the control without jade, and the growth stopped until 10 days, unlike the control showing the decrease in cell biomass. have. This is remarkable in that cell growth continues even when the concentration of nutrient is almost depleted and the concentration in the medium becomes zero compared with the change in the concentration of glucose, a nutrient that acts as the main metabolic substrate in the medium. Do. It is common to show a tendency to stop the growth and decrease the growth rate at this point of time when nutrients are depleted, such as the control without the purgatory. However, when using the purgatory, it shows a different phenomenon. It is assumed to be.

This result is also shown in the figure measuring the dry weight of the cell in Fig. 4 shows the cell growth proliferating at a higher rate than the control after the 3rd day and the same phenomenon as the 3rd after 9 days.

As a result, cells grown close to purgatory in growth media showed a better effect on cell growth than controls that did not. Digitalis lanata plant cells, which have a growth limit of about 10 days, are maintained in a stable state that does not occur due to necrosis of cells without exchanging with new medium even after 10 days when cultured with nephrite. Rather, it is expected that cell proliferation may be an advantageous method for optimizing cell growth and concentration in the production process for the production of useful substances.

All. Effect of Purgatory on Changes in Medium pH

5 is a measure of the change in pH of the medium in the use of purgatory in the growth medium and in the control not used. The control group shows the tendency of pH in the medium to be applied to the general plant cells, which gradually decreases over time and is maintained at a certain level. However, until the seventh day, purgatory showed a similar pattern to that of the control group and increased gradually. This phenomenon was extremely shown in the result of FIG. 6 showing the result of the production medium in which the purgatory powder was added directly to the culture medium. . In the case of the control group without the nephrite powder, the pH was continuously decreased, while the nephrite powder added showed a tendency to maintain the value to some extent, which is an ionic reaction by the mineral component contained in the nephrite. It is guessed whether this acts as the cause.

la. Water quality assay results for purgatory

7 and 8 show the state of the analyzer and the results of the analysis. The data obtained therefrom are summarized in Table 10 and Table 13 below.

Table 10 shows the measurement of each analysis item after 48 hours (Sample 1) was put in a solution of 20g purgatory lumps. In this table, there is no change in each measurement item, and the effect of purgatory does not appear.

Table 11 shows the measured values for each analysis item after 48 hours of curing 20g of nephrite lumps outside the solution (sample 2). Table 13 also showed no change for each analysis item even when purgatory powder was placed outside the solution (sample 4). However, in the case of Sample 3, the data measured for each analysis item of the sample 48 hours elapsed after adding 20 g of nephrite powder in the solution is shown in Table 12. There are many changes that can be seen in this table. As follows.

Cr, Pb, Ni, and Co, which are harmful to the human body, changed significantly after 48 hours. Cr was removed in the absence of purgatory powder, that is, 45.30 mg before the reaction was removed to 0 after 48 hours of purifying powder, and Pb was also completely removed from 13.76 mg to zero. Ni decreased from 51.8 mg to 1.733 mg and Co also decreased from 52.69 mg to 11.94 mg. Mg, one of the essential elements in the human body, increased from 48.6mg to 55.74mg.

The hydrogen ion concentration of distilled water was acidic at pH 3.5, but after the reaction, it was changed to neutral at pH 6.8, and the conductivity rapidly decreased. Adsorption capacity is physically large, about 3-4 mmol.q (equivalent) per gram.

As described above, in the case of Digitalis lanata plant suspension cells cultured for one week with the nephrite near, the growth of about 30% or more was observed without purgatory. This is a good proliferation result, which is rare even in various experiments performed for the proliferation effect on the conventional digitalis lanata plant cells for high concentration culture. In addition, the experiments on how purgatory water affects hard water showed that soft water softened hard water without contact with water. Particularly in the component analysis experiments on water using distilled water, the jadeite powder was precipitated, and after 48 hours in the component analysis, the pH was increased, the Ni and Co components were decreased, the heavy metals were removed and the Mg was increased. An unusual phenomenon was observed that led to the phenomenon of.

As such, purgatory or purgatory powders have not been clearly identified theoretically, but they change the hardness of water without contact with water, and also proliferate more than 30% of digitalis lant plant suspension cells. The removal of heavy metal components harmful to the human body, such as reduction of Ni, Co components, removal of Cr and Pb, is believed to be certain by the efficacy shown in the above-described experimental examples. In addition, the above experimental results suggest the possibility of promoting the metabolism of the human body when taking the purgatory capsule and purifying tablet of the present invention.

Experimental Example 6

In this Experimental Example, in order to examine the effect of UV sterilization effect on the skin of jade water along with the purgatory powder used in the manufacture of the present invention, the experiment on the effect of purgatory powder and purge corn on the UV sterilization effect was carried out. It was performed by requesting the Department of Biology, College of Natural Sciences, University.

The results of the research experiment are described in detail below.

1. Sample

Purified jade powder and chalcedony collected from jadeite mine in Wolgok-ri, Dong-myeon, Chuncheon, Gangwon-do, Korea.

2. Experimental strain and culture

Gram-positive bacteria Bacillus subtilis and Gram-negative bacteria Escherichia. coli and yeast Saccharomyces cerevisiae were selected. Strains of E coli (gram negative) and Bacillus subtilis (gram positive) were cultured in nutrient agar medium (3 g yeast extract, 5 g bacto-peptone, 8 g sodium chloride / 1 liter distilled water). After incubation at 37 ° C. for 24 hours, the colonies were counted. For Saccharomyces cerevisiae (Gerhardt et al., 1994), YPD (3 g yeast extract, 10 g bacto-peptone, 30 g dextrose / 1 liter of distilled water) was used. These strains were shaken for 24 hours at a temperature of 30 ° C. in a shaker incubator.

3. UV irradiation method

1 ml of the strain cultured for 24 hours and diluted in sterile water, gram-positive bacteria Bacillus subtilis and gram-negative bacteria E. coli nutrient solid medium (3g yeast extract, 5g bacto-peptone, 8g sodium chloride, agar 15g / In 1 liter of distilled water, Saccharomyces cerevisiae, a yeast, was inoculated with 0.1 ml each of YPD (3 g of yeast extract, 10 g of bacto-peptone, 30 g of dextrose, 15 g of agar, and 1 liter of distilled water). Each strain inoculated into a solid medium was placed under 50cm of ultraviolet light (115V, 30W, 2537Å tube, etc.) installed in a sterile zone (hand-made line) and examined for each strain. Gram-positive and Gram-coated bacteria were irradiated with UV light for 40 seconds and 1 minute, yeast for 30 seconds and 1 minute, and UV light in the sterile zone was measured with a photometer (Licon LI-189). As a control, the number of cells in a solid medium not irradiated with ultraviolet light was used.

4. Distribution of hemolytic bacteria

형)로 판정하였다.Blood agar plates (BAP) were used to count hemolytic bacterial populations known as pathogenic bacteria in jadeite and corn (Benson, 1994). Blood agar medium (Trypticase soy agar (BBL) 40g / 1 liter of distilled water) was sterilized after cooling to 48-50 ℃ was used by the addition of 5% sterile defiber (Sheep blood). Jade powder and cornmeal were plated on blood agar medium and incubated for 24 hours in a 37 ℃ incubator. The cultured plates were taken out, and the colony morphology and hemolysis characteristics were observed. If a green ring was formed around the colony, it was determined as α-hemolysis. If there is no hemolysis at all,

Type).

5. Measurement of Microbial Communities

end. Microbial community according to the concentration of purgatory

In order to investigate the effects of nephrite and corn on the growth of gram-positive bacteria, gram-negative bacteria, and yeast, nutrients of jade and corn were gradient-cultured in medicinal liquid medium and YPC liquid medium. Corn is mixed with a concentration gradient of 10%, 25%, 50%, 100% (v / v), and jade powder is mixed with a concentration gradient of 5%, 10%, 20%, 30% (w / v). Bacteria and Gram-negative bacteria were counted by incubating at 37 ° C for 24 hours and yeast at 30 ° C for 24 hours. As a control, a liquid medium without corn and jade powder was used.

I. UV protection effect

Irradiation with UV light over time, gram-positive bacteria, gram-negative bacteria and yeast was calculated as a UV blocking effect by converting the number of viable cells in percentage. Gram-positive bacteria and Gram-negative bacteria were plated on three nutrient solid media and irradiated with ultraviolet rays (115V, 30W, 2537Å tube, etc.) and incubated at 37 ° C for 24 hours. Yeast was counted by plating on YPC and incubated at 30 ° C. for 24 hours. The cell count was determined by colony forming unit (CFU) / ml.

[result]

-형)이라 부르고 있다.Hemolytic properties are classified into α- and β types according to the types of hemolytic blood cells formed around colonies of inoculated bacteria on the blood agar medium. If there is no hemolysis at all,

-Is called.

용혈은 190 CFU/ml, 옥수에서 β용혈은 30 CFU/ml,

용혈은 730 CFU/ml로 확인되었다. 옥수에서보다 옥 분말에서 β용혈성 세균이 상대적으로 많이 검출된 결과는 옥수 시료 채취시 연옥분말의 중탕으로 채취하였기 때문이다.α hemolysis is the change of oxyhemoglobin to metemoglobin (methomoglobin), the green ring is formed around the colony, β hemolysis appears to be completely transparent around the colon hemolysis. This hemolysis is caused by hemolytic toxins. Hemolytic colonies have been recognized in purgatory and agate. Β haemolysis in the nephrite powder averaged 930 CFU / ml,

Hemolysis is 190 CFU / ml, corn beta hemolysis is 30 CFU / ml,

Hemolysis was confirmed at 730 CFU / ml. The reason for the relatively higher β-hemolytic bacteria in the jade powder than in the corn was because the corn samples were collected in the bath of the purgatory powder.

2. Measurement of Microbial Communities

Corn was mixed with nutrient liquid medium and YPD liquid medium at a concentration gradient of 10%, 25%, 25%, and 100%. Purgatory powder was mixed with a concentration gradient of 5%, 10%, 20%, and 30%, followed by cultivation, and compared with the control group without addition of purgatory powder and corn. Corn was almost similar in Bacillus subtilis and Saccharomyces cerevisiae compared to the control group without any corn, but E. coli showed more populations than the control group when 10%, 25% and 50% corn was added. It is believed that the trace elements contained in corn affected the growth of E. coli, but not Bacillus subtilis and Saccharomyces cerevisiae. Therefore, it is possible to analyze the microorganisms associated with the growth of microorganisms only by identifying the components and contents of the trace elements contained in corn.

In purgatory powder, Bacillus subtilis, E. coli, and Saccharomyces cerevisiae were all smaller than the control group. There was also a tendency for the population to decrease with higher concentrations of jade powder.

3. UV protection effect

The microorganisms of the normal bacterial flora can be distinguished from resident bacteria and ancestral bacteria. Although resident bacteria remain somewhat persistent, there are a number of factors that affect permanence and its extent. In other words, depending on the climate temperature and humidity can be increased, which can increase the density of skin microorganisms. Children have more Gram-negative and latent pathogens than adults. Therefore, the digestive microorganisms such as Gram-positive bacteria Bacilius subtilis and E. coli were continuously inoculated on the surface of the skin.

The sunscreen effect of purgatory powder and corn was confirmed by the UV sensitivity of microorganisms. Bacillus subtilis, a Gram-positive bacterium, survived for 40 seconds and was 23% and 13%, respectively, in a liquid medium containing 20% and 30% of jade powder, and surviving more than 12% of the control group without adding jade powder. Many. In addition, a similar tendency was observed in a liquid medium containing corn. E. coli showed the highest viable population, and this pattern was more pronounced in the jade powder added liquid medium than in the chalcedony added liquid medium.

In other words, the average of 74% in agate medium with chalcedony and 82% in nutrient solution with jade powder, and the highest survival population was 90% in 10% concentration of jade powder. Regardless of the type of microorganism, the sunscreen effect was higher in purgatory powder than in corn. UV sensitivity of microorganisms is known in the order of Gram-negative bacteria, yeast, spore-forming bacteria, fungi, and is affected by the biological activity, bacteria, moisture, environmental substances, etc. In addition, the photoreaction after ultraviolet irradiation differs depending on the species. Ultraviolet rays have a maximum sterilizing ability at a wavelength of 260 nm, and E. coli and typhoid fever have been reported to die within 10 minutes when irradiated with 50 watts of 15 watts of ultraviolet light (芝 崎 勳, 1983). In the case of Cryptococcus neoformans, the changes in the contents of the cells began to appear at the same time as the cell morphological changes after 10 minutes of treatment. Ultraviolet sensitivity was confirmed in the order of Bacillus subtilis, Saccharomyces cerevisiae, Escherichiacoli. In other words, the UV blocking effect was confirmed in the order of E. coli, Saccharmycs cerevisiae, Bacillus subtilis.

However, when considered in connection with the results of the evaluation of the growth effect by purgatory powder and corn, E. coli, which has a relatively large population, showed the highest blocking effect. In addition, the bactericidal effect of sunlight on E. coli and Proteus vulgaris in the intestinal bacteria was bactericidal in proportion to the average insolation, but this bactericidal effect was related to the result reported by the action of visible light of short wavelength, not ultraviolet light. Therefore, the study of the bactericidal effect according to sunlight should be supplemented.

[conclusion]

용혈은 190 CFU/ml, 옥수에서 β용혈은 30 CFU/ml,

용혈은 730CFU/ml로 확인되었다.1. For purgatory powder, β hemolysis averaged 930 CFU / ml,

Hemolysis is 190 CFU / ml, corn beta hemolysis is 30 CFU / ml,

Hemolysis was confirmed at 730 CFU / ml.

2. The size of microbial community grown in jade powder and corn supplemented medium was changed according to the concentration change of jade powder rather than corn concentration.

3. As a result of analysis of the number of microorganisms surviving after UV irradiation, UV protection effect was higher in jade powder added liquid medium than in jade added liquid medium.

4. The sunscreen effect was confirmed in the order of E. coli, Saccharomyces cerevisiae, Bacillus subtilis.

As described above, the nephrite powder used in the present invention was found to have a very high UV blocking effect.

As described above, taking the product of the present invention, that is, purgatory capsule or purgatory tablet, promotes the medical effect of purgatory substances such as metabolism of the human body, thereby enabling the treatment of diseases (headache, hand and foot, inferior dysfunction and insomnia). There is an excellent effect, and also tablets or encapsulated purgatory has the effect that anyone can always take it with you.

Claims (1)

In the method for producing a drinkable or ingestible product by using the nephrite, the nephrite capsule is prepared by adding 30 wt% of the nephrite powder powdered to a particle size of 400-800Mesh based on the total weight ratio of the known known capsule forming process. The nephrite powder, which was molded and powdered into a particle size of 400-800Mesh, was supplied to a capsule charger at a room temperature of 5 ° C. and a room humidity of 10% to be filled at 200 mg at a rate of approximately 400-0600 CAP / min. Method for producing a capsule using the purgatory, characterized in that.

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