CN108142926A - Lotus wheat drinks health care particle - Google Patents

Abstract

The invention discloses a kind of lotus wheats to drink health care particle, adds in additive mixing granulation after ultramicro grinding by the component of following portions by weight and forms：5 10 parts of lotus leaf, 48 parts of barley seedling, 48 parts of corn stigma, 36 parts of mulberry leaf, 36 parts of gynostemma pentaphylla.Lotus wheat of the present invention drink health care particle have effects that it is more comprehensive, be easy to absorb, conveniently drink, cost of material is low, have both anti-aging, is antifatigue, immunological regulation and antioxidant activity.

Description

Lotus wheat drinks health care particle

Technical field

The invention discloses a kind of health beverages, specifically a kind of lotus wheat drink health care particle.

Background technology

Health beverages type is various in the market, and function, which also respectively has, does not plant, also has respectively for problems various existing for the elderly Kind of health products are available, but preparation process is complicated or function is more single or expensive starting materials, purchase cost are high or curative effect It is limited.As application number 201410298349.8 discloses a kind of gynostemma pentaphylla tea.It is processed by gynostemma pentaphylla, mulberry leaf, cassia seed and radix polygonati officinalis It forms.The gynostemma pentaphylla tea include 45-50 grams of gynostemma pentaphylla, 8-15 grams of mulberry leaf, 8-15 grams of cassia seed, 15-30 grams Radix polygonati officinalis decocts each ingredient with water, and cold heavy, centrifugation, filtering, addition sweetener add water constant volume, high-temperature instantaneous kills after merging filtrate Bacterium, packing, product as of the present invention, it with dispelling wind and heat from the body, clearing liver kidney-nourishing, improving eyesight, defaecation, moisturize quench the thirst, reducing blood sugar and blood lipid, The functions such as calming soporific, preparation process is complicated and is mainly used for lower hyperlipidemia, hypertension, hyperglycemia；For another example application number 201510339643.3

A kind of corn stigma tea bag is disclosed, is related to field of beverage.The corn stigma tea bag is counted in parts by weight, each component Composition is as follows：5~10 parts of corn stigma, 12~20 parts of hawthorn, 3~8 parts of lotus leaf, 25~40 parts of green tea, 2~7 parts of chrysanthemum, cassia seed 0.2~1 part and 1~2 part of Radix Glycyrrhizae.The corn stigma tea bag that the invention provides can utilize the corn stigma that script abandons, And tea bag nutritive value obtained is high, in good taste.It mainly has anti-aging, antifatigue, immunological regulation, anti-oxidant and tune Fat, decompression, hypoglycemic and other effects.But tea bag form can be such that liposoluble constituent is lost together with medicine quarrel, cause liposoluble constituent Waste.

Invention content

The purpose of the present invention is to solve above-mentioned technical problem, provide a kind of effect more comprehensively, be easy to absorb, conveniently It drinks, cost of material is low, have both anti-aging, is antifatigue, the multi-functional lotus wheat drink health care particle of immunological regulation and antioxidant activity.

The lotus wheat drinks health care particle, and additive mixing granulation is added in after ultramicro grinding by the component of following portions by weight It forms：5-10 parts of lotus leaf, 4-8 parts of barley seedling, 4-8 parts of corn stigma, 3-6 parts of mulberry leaf, 3-6 parts of gynostemma pentaphylla.

It is formed by the component of following portions by weight through ultramicro grinding, mixing granulation：6-9 parts of lotus leaf, 5-8 parts of barley seedling, jade 5-8 parts of rice palpus, 4-5 parts of mulberry leaf, 4-5 parts of gynostemma pentaphylla.

Further, each component is first crushed to less than 10 μm nanoscale Ultramicro-powders respectively, then adds in additive mixing Granulation is squeezed into granule through dry method after uniformly.

In the present invention, lotus leaf, barley seedling, corn stigma, mulberry leaf and gynostemma pentaphylla compatibility are selected, all components are medicine food two With, it is without any side effects, it eats harmless more, can take for a long time.Lotus leaf is mild-natured, bitter, Return liver, spleen, stomach, 2010 editions《In State's pharmacopeia》It records, lotus leaf has clearing away summer-heat and eliminating dampness, cooling blood and hemostasis and other effects, is mainly used for hot summer weather polydipsia, splenasthenic diarrhea, blood-head is spat Nosebleed etc..《Compendium of Materia Medica》" lotus leaf takes it, makes us thin bad ", " hair tonic vigour, benefit help taste " are recorded to the medical value of lotus leaf.Lotus Leaf main component is the ingredients such as alkaloid, flavones, essential oil, vitamin C, organic acid, and lotus leaf chemical constitution study is found in recent years, It mainly contains a variety of chemical compositions such as alkaloids, flavonoids, volatile oil and multivitamin, trace element, waits ingredients, With many-sided pharmacological activity, such as it is lowering blood-fat and reducing weight, anti-oxidant, anti-aging, antibacterial.With clearing away summerheat, sending up the lucid yang, cool blood The various functions such as hemostasis, are clinically mainly used for hot summer weather polydipsia, summer heat damp diarrhea, blood-head tells nosebleed, uterine bleeding of having blood in stool.Lotus in lotus leaf Contain in leaf alkali there are many effectiveization fat alkaloid, fat that can be effectively in decomposer, and powerful excrete.Nuciferine energy In intrepid densely covered human body intestinal wall, one layer of fatty isolation film is formed, fat absorption is prevented, prevents fat accumulation.It can improve greasy Eating habit has effects that stronger grease repels you is allowed to generate dislike gradually to meat or fish stodge.Lotus leaf lotus Leaf has antipyretic, antibacterial, spasmolysis, promotes intestines peristalsis discharge toxin, draining diuresis and other effects.Ministry of Health of the People's Republic of China Defend the list that lotus leaf was included in second batch " be both food and drug " in 1991.Recent domestic scholar carries out lotus leaf System research, the pharmacological action of lotus leaf and application prospect are more paid close attention to by people.The lotus leaf additive amount is controlled in 5-10g, Resource can be excessively caused, it is very few to influence weight-reducing, lipid-loweringing, anti-oxidant, anti-aging, antibacterial and other effects.

Barley seedling acrid flavour trembles with fear, is nontoxic.Ming Dynasty's Li Shizhen (1518-1593 A.D.)《Compendium of Materia Medica》It records：" cure mainly the wine poison that disappears, sudden-heat, wine subcutaneous ulcer, mesh Huang, and mash rubbing filter and take it, and solve disease due to noxious agents produced by various parasites, liquor filter clothes, except unhappiness, except when disease it is fanatic, move back septum pectorale heat, sharp small intestine.Make seedling Food, very beneficial color "." barley is sweet in flavor, it is mild-natured, there is swollen dietotherapy, disperse accumulation feed, flat stomach to quench the thirst, relieve summer heat except in heat, QI invigorating tune, it is wide Chest air, qi-restoratives are bad, strengthen blood vessels, beneficial color, real the five internal organs, the work(for changing paddy food ", it is seen then that barley seedling has very high nutritive value And medical value.Barley seedling richness house albumen is hard, vitamin, mineral an ancient egg-shaped, holed wind instrument, chlorophyll, Polyphenols active material, it have it is anti-it is fat, Anticancer, toxin expelling, beauty and other effects are a kind of most natural health non-defective unit and basic foods.Vitamin, minerals contained by barley seedling And protein is the essential nutrients of the mankind and animal, contains beta carotene, vitamin B in barley seedling juice₁、B₂、B₆、B₁₂Pantothenic acid and Folic acid and mineral element potassium, calcium, iron, phosphorus, magnesium and chlorophyll, amino acid, protein, cellulose and biological enzyme etc..Barley seedling is also There is " three high two is low ", high protein, high dietary-fiber, homovitamin, low fat, low sugar.Research shows that：It is oral daily A small amount of wheat seedling powder can effectively improve sleep, repair memory, improve immune function, promote wound healing, reinforcement quality, alleviate The various uncomfortable reactions of human body safeguard heart, maintain internal normal blood picture index, promote body detoxification, delaying human body caducity.Institute The control of barley seedling additive amount is stated in 4-8g, it is very few to influence to improve sleep, repair memory, improve immune function, promote wound Healing, reinforcement quality alleviate the various uncomfortable reactions of human body, safeguard heart, promote body detoxification, delaying body aging and dimension Hold normal blood picture index and other effects in vivo.

Corn stigma sugariness is put down, and returns liver and kidney channel.《Ministry of Health of the People's Republic of China's medicinal material standard》1985 editions (one) will Corn stigma is included by medicinal herbs most in use.Recent study finds that the chemical substance in corn stigma is very abundant such as carbohydrate, saponin(e, Huang Ketone, inorganic elements, amino acid, alkaloid, volatile chemical component, organic acid and multivitamin, trace element etc. are a variety of Chemical composition, etc., it is advantageous to health, available for treatment difficult urination, diabetes, hypertension, gall stone, hyperlipidemia etc. A variety of diseases.Corn stigma also has diuresis, can increase internal chloride discharge rate.The corn stigma additive amount control is in 4- 8g, it is very few to influence diuresis, hypoglycemic, drop blood viscosity, prevention hypertension, gall stone, hyperlipidemia and other effects.

Mulberry leaf are bitter, sweet, cold, return lung, Liver Channel, have the effect of dispelling wind and heat from the body, clearing away the lungheat and moisturizing, flat liver improving eyesight, cooling blood and hemostasis, It is one of conventional Chinese medicine.《Compendium of Materia Medica》It records, mulberry leaf are the medicine of brothers Yang Ming, and juice decocts Dai Ming, can only be quenched one's thirst, item long hair. Modern pharmacology proves that mulberry leaf have hypoglycemic, blood pressure lowering, antibacterial and a variety of pharmacological activity such as antiviral.Chinese Han dynasty《Legendary god of farming's sheet Grass warp》Middle mulberry leaf are referred to as " Radix Rhodiolae ".Important plant resources of the mulberry leaf as medicine-food two-purpose close in recent years as domestic and foreign scholars Note and the hot spot of research.It is research shows that more containing 1- deoxidations open country mycin, a variety of flavone compounds, amino acid, mulberry leaf in mulberry leaf The bioactive ingredients such as sugar have hypoglycemic, reducing blood lipid, anti-oxidant, protection cardiac muscle, anti-aging, anti-inflammatory antibacterial and improvement just The pharmacological actions such as secret, skin maintenance, mulberry leaf are uniquely to contain blood sugar reducing component 1- deoxidations open country mycin Chinese medicine.Mulberry leaf is in tradition Medicine contains abundant amino acid, cellulose, minerals and flavones and multivitamin, trace element in discovered in recent years mulberry leaf Etc. a variety of chemical compositions, various bioactivators are waited, there is hypoglycemic, reducing blood lipid, anti-oxidant, anti-aging, resisting stress, change Kind constipation, the anti-inflammatory pharmacological actions such as antibacterial, prevent the chronic diseases such as diabetes, atherosclerosis, obesity with the mankind and prolong Year lengthens one's life closely related.The mulberry leaf must additive amount control in 3-6g, it is very few can influence hypoglycemic, reducing blood lipid, it is anti-oxidant, delay Aging, resisting stress improve constipation, are anti-inflammatory antibacterial and other effects.

Gynostemma pentaphylla bitter, micro-sweet, cool in nature, nontoxic, return lung, spleen, kidney channel.Gynostemma pentaphylla as edible wild herbs eat first recorded in《It sends relief to a famine area Book on Chinese herbal medicine》,《Compendium of Materia Medica》Its entitled " Japanese Cayratia Herb " is recorded, there is clearing heat and detoxicating, relieving cough and reducing sputum, tranquilizing the mind invigorating the spleen, tonifying Qi to promote the production of body fluid Effect.Gynostemma pentaphylla has the title of " the elixir grass " of " south ginseng " and mystery.1986, gynostemma pentaphylla was classified as by the Department of Science and Technology It is primary " rare traditional Chinese medicine " leaved for development in Spark Program, 2002, health products register is included in by the Ministry of Public Health.It is main Ingredient is a variety of gypenosides such as ginsenoside Rb, has preventing phlegm from forming and stopping coughing, invigorating the spleen qi-regulating, qi and activate blood circulation, promotes the production of body fluid to quench thirst, detoxifies The effect of dampness removing.Gynostemma pentaphylla also containing saponin(e, flavonoids, carbohydrate, terpene and multivitamin, a variety of chemistry such as trace element into Point, there is the pharmacological actions such as reducing blood lipid, antitumor, protection liver, pre- anti-aging, raising immunity, current pharmacological research hair Existing gypenosides are with reducing blood lipid, liver protection, anti-inflammatory, anti-oxidant, adjusting is immune, antitumor and protects the pharmacology such as cardiovascular and cerebrovascular Activity, gynostemma pentaphylla can slowly melt away thick body fat.Gynostemma pentaphylla additive amount control is very few to influence to subtract in 3-6g Fertilizer, adjusting blood fat, antitumor, protection liver, pre- anti-aging, anti-inflammatory, anti-oxidant, adjusting is immunized and protection cardiovascular and cerebrovascular is equivalent Fruit.

Above-mentioned 5 kinds of medicines food is with micro-powder of Chinese herbs compatibility, and the contained water solubility beneficial to human body and fat-soluble nutrients are into graded It is kind complete, effect enhancing or complementary, throughout each organ-tissue of human body, weight-reducing, adjust blood fat, hypoglycemic, drop blood viscosity, Antitumor, anti-oxidant, anti-inflammatory, antibacterial, antibacterial, resisting stress promote body detoxification, adjust immune, protection cardiovascular and cerebrovascular, protection liver Dirty, prevention slows down aging, improves constipation, diuresis, prevention hypertension, prevention gall stone, improve sleep, repair memory, alleviate Normal blood picture index etc. etc. all has very good effect in vivo for the various uncomfortable reactions of human body and maintenance.As medicine-food two-purpose medicine Material without any toxic side effect or adverse reaction, takes safety, and long term usage is harmless, and it is especially senile can to become some diseases Disease and chronic disease, the treatment drug and health product of sub-health population.In addition, although dietary fiber cannot be by human consumption Road enzyme decomposes, but dietary fiber is that healthy diet is indispensable, and fiber plays important on digestive system health is kept Role is referred to as " the seventh-largest nutrient " of the mankind.Fiber can clean wall of digestive and enhancing digestive function, and fiber simultaneously can be dilute The removal of the carcinogen and noxious material in food is released and accelerated, protect fragile alimentary canal and is prevented colon cancer.Fiber can Slowing down the enough dietary fibers of digestion rate intake can be with prevention of cardiovascular disease, cancer, diabetes and other chronic disease. Most rapid drainage cholesterol, so the blood glucose and cholesterol that can allow in blood are controlled in optimal level.Lotus wheat drinks health care Except " nutrient " that with the dietary fiber in addition to absorbed active constituent can be exactly human body needs in 5 kinds of medicinal materials in particle.

Further, inventor does not use the method for decocting and extracting, but employs ultramicro grinding, and each component is distinguished powder 10 μm are broken to hereinafter, cell is made to reach shell-broken effect, is on the one hand conducive to digest and assimilate, gives full play to drug effect comprehensively, on the other hand Dietary cellulosic needed by human can be supplemented, in addition, when being made into granule Instant Drinks, in suspension shape, melting is good, favorably In drinking, be easy to receive inhale, there is no the dregs of a decoction are abandoned the problem of.

According to the needs of mouthfeel, technique, additive can be added in into above-mentioned component, including but not limited to water-soluble binds Agent (such as carboxymethyl cellulose), sweet taste corrigent (such as stevioside), fragrant corrigent (such as lemon).

Advantageous effect：

Lotus wheat drink health care particle nutritional ingredient of the present invention is easy to absorb, and not comprising any harmful ingredient, is not inhaled It is conveniently drunk, cost of material is low, some raw material are waste profit for supplementing dietary cellulosic needed by human the part of receipts With having both that anti-aging, antifatigue, immunological regulation and antioxidant activity etc. are multi-functional, and ultramicro grinding to less than 10 μm micro mists is not only The Multiple components in 5 medicinal materials can be enable directly to be absorbed by organisms, the soluble cellulose largely retained is to human body microecosystem System balance is very useful, suitable for all groups, especially suitable for the elderly, is weak from one's illness and sub-health population, will not Any adverse reaction occurs, can take for a long time.

Specific embodiment

Embodiment 1

10 parts of lotus leaf, 4 parts of barley seedling, 3 parts of corn stigma, 3 parts of mulberry leaf, 5 parts by weight of gynostemma pentaphylla.

Preparation method is:Each medicinal material is first distinguished into ultramicro grinding to 10 μm hereinafter, then mixing 5 groups in more than ratio again Part, it adds 0.05 part of carboxymethyl cellulose, 0.02 part of stevioside, 0.02 part of citric acid and squeezes and pelletize through dry method after being uniformly mixed It forms, obtains lotus wheat drinking particle agent 1.

Embodiment 2

8 parts of lotus leaf, 6 parts of barley seedling, 4 parts of corn stigma, 4 parts of mulberry leaf, 3 parts of gynostemma pentaphylla.Preparation method is:First by each medicinal material Ultramicro grinding is to 10 μm hereinafter, then adding 0.05 part of carboxymethyl cellulose, sweet tea in more than ratio 5 components of mixing again respectively 0.02 part of chrysanthemum glycosides, 0.02 part of citric acid are pelletized after being uniformly mixed through dry method extruding, obtain lotus wheat drinking particle agent 2.

Embodiment 3

6 parts of lotus leaf, 8 parts of barley seedling, 4 parts of corn stigma, 3 parts of mulberry leaf, 4 parts of gynostemma pentaphylla.Preparation method is:First by each medicinal material Ultramicro grinding is to 10 μm hereinafter, then adding 0.05 part of carboxymethyl cellulose, sweet tea in more than ratio 5 components of mixing again respectively 0.02 part of chrysanthemum glycosides, 0.02 part of citric acid are pelletized after being uniformly mixed through dry method extruding, obtain lotus wheat drinking particle agent 3.

Embodiment 4

5 parts of lotus leaf, 4 parts of barley seedling, 8 parts of corn stigma, 6 parts of mulberry leaf, 6 parts of gynostemma pentaphylla.

Preparation method is:Each medicinal material is first distinguished into ultramicro grinding to 10 μm hereinafter, then mixing 5 groups in more than ratio again Part, it adds 0.05 part of carboxymethyl cellulose, 0.02 part of stevioside, 0.02 part of citric acid and squeezes and pelletize through dry method after being uniformly mixed It forms, obtains lotus wheat drinking particle agent 4.

Melting is tested：Example lotus wheat drink health care particle 1 bag (5g) heats water 200mL, stirs 5min, sees immediately The melting of particle is examined, as a result this product is all dissolved, in suspension shape.

Efficacy experiments：

1 lotus wheat drinks the antifatigue effect to mouse

Lotus wheat drink is studied to the antifatigue effect of mouse, divides lotus wheat drinking particle agent 1 to 3 dosage groups i.e.：100mg/kg.d、 To intragastric administration on mice, control group is physiological saline by 200mg/kg.d, 400mg/kg.d.It is swum after common 42d, last feeding 30min Swimming experiment, measures the indexs such as exercise tolerance, blood lactase acid (LAC), blood urea nitrogen (BUN) at resist oxygen lack experiment, the results show that 3 agent The lotus wheat drink of amount can significantly improve exercise tolerance and hypoxia endurance time, reduce the content of LAC and BUN after movement, improve flesh, liver Glycogen content, it is seen that lotus wheat kitchenware has good antifatigue effect.

Method healthy mice 200, weight 18-22g, it is male female unlimited, it is randomly divided into control group (NC) and experiment I group (lotuses Wheat drinks suspension I), experiment II groups (lotus wheat drinks suspension II) test III groups (lotus wheat drinks suspension III), every group 40 small Mouse.After lotus wheat drinking particle agent 1 is made into the dose suspension of 100mg/kg.d, 200mg/kg.d, 400mg/kg.d with physiological saline Respectively to experiment I groups, experiment II groups, experiment III 3 experimental mice gavages of group, 1 time a day, each 0.5mL, control group is used Every group of mouse is randomly divided into 4 groups after continuous 42d by the physiological saline gavage of equivalent, every group 10, carry out respectively swimming test, Resist oxygen lack experiment measures blood lactase acid and blood urea nitrogen variation and the influence experiment to hepatic glycogen, muscle glycogen content.

1.1 swimming test

It is 30 ± 2 DEG C that every group of 10 mouse, which are respectively put into temperature, and the swimming trunk of depth of water 40cm records small without swimming with a load attached to the body Mouse starts swimming to the time of sinking to the water surface until it cannot emerge of being perfectly exhausted, swimming time of the time for mouse. The results show that experiment I groups (lotus wheat drink suspension I), experiment II groups (lotus wheat drinks suspension II), (the lotus wheat drink suspension of experiment III groups Liquid III) and control group (NC) the average swim time be respectively 237 ± 35.82min, 255 ± 49.08min, 286 ± 49.79min, control group are 298 ± 33.58min, it is seen that lotus wheat drink suspension can extend mouse swimming time, can improve The exercise tolerance of mouse has antifatigue effect, and dose-effect relationship is presented.

1.2 hypoxia endurance test

Every group of 10 mouse are respectively placed in the wide-mouth bottle of the 250ml equipped with 10g soda limes, 1 every bottle, seal bottleneck Timing at once afterwards.The death time of mouse is observed and recorded, is the hypoxia endurance time of mouse.The results show that (lotus wheat drinks experiment I groups Suspension I), experiment II groups (lotus wheat drinks suspension II), experiment III groups (lotus wheat drinks suspension III) and control group (NC) it is resistance to scarce The oxygen time is respectively 23.6 ± 3.44min, 25.1 ± 2.99min, 26.9 ± 2.65min and 28.6 ± 2.12min.It can be seen that lotus wheat Drink suspension can extend mouse hypoxia endurance time, can improve the exercise tolerance of mouse, have antifatigue effect, and presentation amount Effect relationship.

1.3 Serum lactic acid contents measure experiment

Every group of 10 mouse by tail portion are taken a blood sample, are contained with parazon determination of color mouse quiescent stage blood lactase acid Amount.Again by " swimming test " method, it is 30 ± 2 DEG C that every group of 10 mouse are respectively put into temperature, the swimming trunk of depth of water 40cm without Swimming with a load attached to the body 30min takes out mouse, and 15min and 50min uses parazon determination of color blood lactase acid after swimming Content.The results show that before swimming and after swimming, 3 time Serum lactic acid contents of 15min and 50min are respectively to test I group (lotus wheats Drink suspension I) 19.16 ± 1.59 45.87 ± 2.31 34.83 ± 2.18mmol/L, experiment II groups (lotus wheat drinks suspension II) 18.69 ± 2.03 39.46 ± 2.09 28.69 ± 2.21mmol/L, experiment III groups (lotus wheat drinks suspension III) 18.88 ± 1.75 38.32 ± 2.42 24.31 ± 2.58mmol/L, control group (NC) 19.56 ± 1.76 50.81 ± 1.98 42.87 ± 2.98mmol/L.It can be seen that the Serum lactic acid content of each dosage group and NC indifferences before swimming, 25min measurement results are shown after swimming, Test I groups (lotus wheat drinks suspension I), experiment II groups (lotus wheat drinks suspension II), experiment III groups (lotus wheat drinks suspension III) 3 The Serum lactic acid content of experimental group is significantly lower than NC groups；And the LAC contents of each dosage groups of 50min reduce more with NC groups ratio after swimming Add apparent, it is seen that lotus wheat drink suspension can reduce the lactic acid production that mouse generates by swimming, and dose-effect relationship is presented.

1.4 blood urea nitrogen assays are tested

It by " Serum lactic acid content measures experiment " method, is taken a blood sample by tail portion, mouse is measured with diacetyl monoxime-thiosemicarbazide method Quiescent stage blood urea nitrogen content.The 60min that swims takes out mouse, 50min tail portions blood sampling diacetyl monoxime-thiosemicarbazide after swimming Method measures blood urea nitrogen content.The results show that blood urea nitrogen content is respectively, experiment I groups (lotus wheat drinks suspension I) 5.75 ± 0.96 7.86 ± 0.89mmol/L, experiment II groups (lotus wheat drinks suspension II) 5.32 ± 1.01 6.25 ± 1.02mmol/L, examination Test III groups (lotus wheat drinks suspension III) 5.27 ± 0.93 5.99 ± 0.77mmol/L and control group (NC) 5.38 ± 0.99 8.45 ± 0.76mmol/L is the results show that each dosage group and control group mice blood urea nitrogen content indifference during quiescent stage, acutely After movement (swimming 60min) 50min, control group mice blood urea nitrogen content increases, and is significantly higher than each lotus wheat drink dosage group

1.5 hepatic glycogen, muscle glycogen content measure experiment

By every group of 10 mouse, mouse takes hindlimb muscle and liver, normal saline flushing immediately after cervical dislocation is put to death respectively Afterwards, blotted with filter paper, carry out flesh, liver glycogen content measure.The results show that experiment I groups (lotus wheat drinks suspension I) 2.458 ± 0.2247.079 ± 0.691mg/g, experiment II groups (lotus wheat drinks suspension II) 2.642 ± 0.3197.758 ± 0.644mg/g, examination Test III groups (lotus wheat drinks suspension III) 2.944 ± 0.7177.579 ± 0.309mg/g and control group (NC) 2.115 ± 0.0151 6.578±0.825mg/g.The result shows that as it can be seen that each dosetest group mouse hepatic glycogen and Body development content are above NC, two The muscle glycogen content of high dose test group is significantly higher than NC.

2 lotus wheats drink the immunoregulation effect to mouse

Immunoregulation effect of the lotus wheat drink to mouse is inquired into, by 30 mouse (male female unlimited), is randomly divided into 3 groups, every group, 10.It is filled respectively by 100mg/kg.d, 200mg/kg.d, 400mg/kg.d to the suspension of intragastric administration on mice lotus wheat drinking particle agent 1 Stomach volume 0.1ml/10g weight, 1 time/d, continuous 8d.

The mouse lymphocyte transformation experiment of 2.1 ConA inductions

1640 culture medium filtering with microporous membrane degerming, add in 10% calf serum, 1% glutamine (200mg/mL), Penicillin (100mg/mL), streptomysin (100mg/mL) and 2 mercapto ethanol (5 μm of ol/mL), with sterile HC1 (1mol/L) or nothing ((1mol/L) adjusts pH to 7.0-7.2 to bacterium NaOH, and complete culture solution is made；Make solvent with the PBS buffer solution of 7.2-7.4, now match MTT solution (5mg/mL)；With 3.5% sterile NaHCO₃Sterile Hansks liquid pH is adjusted to 7.2-7.4；It is configured to distilled water The ConA liquid of 100ml, after filtration sterilization, -20 DEG C of preservations；4mLHCI (1mol/L) is added in 96rnl isopropanols, acidity is made Aqueous isopropanol.Each group animal continuous gavage was put to death to the 8th day per mouse cervical dislocation, sterile to take spleen, appropriate sterile in filling In the small plate of Hanks liquid, gently spleen is torn up with son is taken the photograph, individual cells suspension is made, the filtering of 200 mesh is multiple with Hanks liquid It is suspended in 2rnL complete culture solutions after washing, centrifugation, calculates living cells quantity with platform phenol method meter, adjustment cell concentration is 2×10⁶A/mL, a point holes are put into culture hole, and per hole 1mL, a hole adds 50 μ LConA liquid, and another hole is put and contained as control 5%CO₂, cultivate 72h in 37 DEG C of incubators, culture terminates preceding 4h, and supernatant 0.7mL is gently sucked per hole, adds in 0.7rnL not 1640 culture medium containing calf serum, while 50mLMTT liquid is added in, continue to cultivate 4h.Culture terminates, and it is acid that 1rnL is added in per hole Isopropanol blows and beats mixing, is completely dissolved purple crystal, moves into 1rnL cuvettes, and light is measured at spectrophotometer 570nm Density.

With the mouse spleen lymphocyte transformation experiment (mtt assay) that ConA is added to induce, the OD value in ConA holes is added to subtract not The OD value in ConA holes is added to represent the proliferative capacity of lymphocyte, the results show that lotus wheat drinks 100mg/kg.d, 200mg/kg.d It is respectively 0.014 ± 0.012,0.063 ± 0.039 and 0.077 ± 0.041 with 3 dosage group optical density of 400mg/kg.d, just Normal control group is 0.014 ± 0.006.It can be seen that middle and high 2 dosage groups have the work of the ability of enhancing mouse lymphocyte proliferation With.

2.2 serum hemolysin measure-half hemolytic value (HC₅₀) measure

When each group animal continuous gavage is to 3d, 2% (v/v) sheep red blood cell (SRBC) suspension 0.2mL is injected intraperitoneally per mouse.5d (the phases Between do not stop gavage) after, mouse takes blood in centrifuge tube through eye socket, place 1h, 3500r/min centrifugation 15min, collect serum, Serum dilutes 320 times with normal saline buffer solution.Serum 1rnL after dilution is put in test tube, sequentially adds 10% (v/v) SRBC 0.5rnL, complement 1rnL (press 1 with physiological saline：10 dilutions), separately set the control tube of not increase serum (with physiological saline replacement).It puts After keeping the temperature 30min in 37 DEG C of waters bath with thermostatic control, ice bath terminates reaction.2000r/min centrifuges 10min.Take supernatant 1rnL, Dou Shi examination Agent 3rnL takes 10% (v/v) SRBC 0.25rnL to add Dou Shi reagents to 4rnL in test tube, in conduct in another test tube The half hemolytic value of SRBC.Abundant mixing after placing 10min, makees blank with control tube at 540nm, measures each Guan Guang respectively Density value.The amount of hemolysin is with half hemolytic value (HC₅₀) represent, it is calculated by following equation：Half hemolytic value (the HC of mouse₅₀) The optical density * extension rates of=sample cell optical density/SRBC half hemolysis.The results show that lotus wheat drinks 100mg/kg.d, 200mg/ Half hemolytic value (the HC of 3 dosage group mouse of kg.d and 400mg/kg.d₅₀) be respectively 140.46 ± 51.02,160.49 ± 88.82 and 184.22 ± 106.38, Normal group is 63.15 ± 57.25.It can be seen that lotus wheat drink 3 dosage groups of solution all have Promote the effect of mouse antibodies generation.

2.3 mouse carbonic clearances are tested

India ink is made again with normal saline dilution 4 with prepared Chinese ink, weighs 1.0gNa₂CO₃Distilled water is added to match to 1000mL It is set to 0.1%Na₂CO₃Solution.When 3 groups of mouse continuous gavages are to 8d, the injection injection prepared Chinese ink (0.1mL/10g per caudal vein Bw), timing immediately after injection, 0.5,6min takes 20 μ L of blood from angular vein clump respectively after injection, is added to 2mLNa₂CO₃Solution In, with spectrophotometric instrument meter at 600nm wavelength densitometric value, with 0.1%Na₂CO₃Solution is as blank control.Cervical vertebra takes off Mortar puts to death mouse, takes liver, spleen, blots organ surface blood stains with filter paper and weigh.Index K is cleaned up by following equation calculating：It is wide Clear index K=(1ogOD₅₀-logOD₆)/(t₆-t_0.5).The results show that lotus wheat drink 100mg/kg.d, 200mg/kg.d and Half hemolytic value (the HC of 3 dosage group mouse of 400mg/kg.d₅₀) it is respectively 0.082 ± 0.012,0.091 ± 0.022 0.098 ± 0.011, Normal group is 0.076 ± 0.016, it is seen that, lotus wheat, which drinks 3 dosage groups, all has enhancing mouse carbon exterior feature The effect of clear ability.

3 lotus wheats drink activity of fighting against senium

10% lotus wheat drink solution is prepared：Lotus wheat drinking particle 300g is taken, respectively with 1000mL water ultrasonic extraction three times, every time 1h, merging filtrate, 3500rpm centrifugations 15min, 0.45 μm of filter membrane filtration are adjusted to 3000ml, are sub-packed in 10mL ampoules, seal Mouthful, 100 DEG C of sterilizing 30min are prepared into 10% lotus wheat drink solution.

To investigate the present invention to hydrogen peroxide (H₂O₂) induction hepatoma cell strain (HepG2) cellular damage model anti-aging Activity has studied lotus wheat drink to H₂O₂The activity of fighting against senium of HepG2 cellular damage models is induced, the results show that lotus wheat drink solution exists H during a concentration of 1000 μ g/ml₂O₂HepG2 cell survival rates under effect are maximum, and lotus wheat kitchenware has more significant anti-cell aging Effect.

3.1 HepG2 cell culture and processing

HepG2 cell strains are provided by ATCC, are stored in liquid nitrogen, with containing 10% newborn calf serum, 100U/mL moulds The RPMI1640 medium cultures of element, 100 μ g/ml streptomysins.It is placed in 37 DEG C of saturated humidities, containing 5%CO₂With the perseverance of 95% air It is cultivated in incubator, directly piping and druming is passed on without trypsin digestion, is tested when cell reached for 10~20 generation.

H₂O₂Induce the foundation of hepatoma Hep G 2 cells damage model：HepG2 cells are with 1.5 × 10⁴The density of cells/well connects Kind is in 96 orifice plates, and adherent to give 10% lotus wheat drink solution treatment afterwards for 24 hours, concentration is respectively 1000 μ g/ml, 500 μ g/ml, 250 μ g/ml, 125 μ g/ml, 62.5 μ g/ml, 31.25 μ g/ml, 0 μ g/ml give 800 μM of hydrogen peroxide treatments after 2h is administered, and handle Period whole process is administered, and blank control group and positive control CAPE is set to compare.

After hydrogen peroxide treatment 2h, make its final concentration of 0.5mg/mL after MTT is added in per hole, after 37 DEG C are incubated 4h, per hole Add in 100 μ L lysates, it is to be crystallized be completely dissolved after, absorbance value is measured at 570nm wavelength with microplate reader.

3.2 mtt assay detect cell viability

HepG2 cells 1 × 10⁴The cell suspension of cells/mL is inoculated in 96 well culture plates with every 50 μ L of hole.Overnight incubation After cell is adherent, addition sample continues culture, and (concentration is respectively 1000 μ g/ml, 500 μ g/ml, 250 μ g/ml, 125 μ g/ for 24 hours Ml, 62.5 μ g/ml, 31.25 μ g/ml, 0 μ g/ml), the fresh culture culture 4h of the MTT containing final concentration of 5mg/mL is added in, Per hole add in 100 μ L lysates, it is to be crystallized be completely dissolved after, absorbance value is measured at 570nm wavelength with microplate reader.It calculates thin Intracellular growth survival rate.

The result shows that HepG2 is in H₂O₂Cell survival rate under effect is 78.30%, and lotus wheat drinks solution in 0 μ g/ of concentration Under the action of ml, 31.25 μ g/ml, 62.5 μ g/ml, 125 μ g/ml, 250 μ g/ml, 500 μ g/ml, 1000 μ g/ml, survival rate point It Wei 15.17%, 65.70%, 72.40%, 74.60%, 75.60%, 90.70%, 107.80%.It is positive to survive with reference to CAPE Rate is 93.40%.Lotus wheat drink is in a concentration of 1000 μ g/ml to HepG2 in H₂O₂Cell survival rate under effect is maximum.As it can be seen that Lotus wheat drinks solution to H₂O₂The liver cancer cells of induction have significant protective effect, enhance with the raising antioxidation of concentration, and There is apparent anti-cell aging higher than the positive antioxidation with reference to CAPE, lotus wheat kitchenware is better than after 500 μ g/ml in concentration Effect.

4 lotus wheats drink antioxidant activity

10% lotus wheat drink solution is prepared same《3 lotus wheats drink activity of fighting against senium》.

Using 1,1 one diphenyl picryl phenylhydrazine (DPPH) free radical, hydroxyl radical free radical, ultra-oxygen anion free radical, reduction The antioxidant activity of 4 kinds of scale-model investigation lotus wheat drinks of power, result of the test show various concentration lotus wheat drink to DPPH free radicals, hydroxyl certainly There is certain antioxidant activity by base, ultra-oxygen anion free radical, there is certain reducing power, there is apparent dose-effect passes System.

The measure of 4.1 scavenging ability of DPPH free radical

DPPH free radicals are a kind of proton free radicals centered on nitrogen of stabilization, are widely used in grinding for antioxidant In studying carefully, have many advantages, such as stable, sensitive and easy.

Take 0.02 respectively, 0.04,0.06,0.08, be suspended made of the lotus wheat drinking particle agent 2 of 0.10mg/mL various concentrations Liquid detects absorbance at 517nm.With 0.002,0.004,0.006,0.008, the v of 0.010mg/mL various concentration gradients_cMake For positive control.The absorbance that 2mL distilled water adds the DPPH solution of 2mL0.1mmol/L to be surveyed is A_o；Same volume sample liquid adds nothing The absorbance that water-ethanol is surveyed is A_j；The absorbance that same volume sample liquid adds the DPPH solution of 0.1mmol/L to be surveyed is A_i.It removes Rate (%)=[1- (A_i-A_j)/A_o]×100.The results show that suspension 0.02 made of lotus wheat drinking particle agent 2,0.04,0.06, 0.08th, the clearance rate of 0.10mg/mL various concentrations is respectively 38.6%, 53.2%, 71.4%, 86.5%, 92.3%

4.2 remove the measure of hydroxyl radical free radical ability

0.75mmol/L Phen test solutions 1mI is taken in test tube, sequentially adds PBS test solutions (pH 7.40) 2mL, is distilled Water 1mL after abundant mixing, adds in 0.75mmol/L ferrous sulfate solution 1mL, and mixing adds the dioxygen that mass fraction is 0.12% Water 1mL, 37 DEG C of water-baths keep the temperature 60min, its absorbance A are measured at 536nm_pHydrogen peroxide is replaced with distilled water, measures its extinction Spend A_b；Respectively with 0.2,0.4,0.6,0.8, the CPCS solution of 1.0mg/mL various concentrations replace distilled water 1mL, measure its extinction Spend A_s.With 0.2,0.4,0.6,0.8, the Vc of 1.0mg/mL various concentration gradients do positive control.Clearance rate (%)=(As- Ap)/(Ab Ap)×100.The results show that lotus wheat drink solution 0.02,0.04,0.06,0.08,0.10mg/mL various concentrations it is clear Except rate is respectively 38.6%, 53.2%, 71.4%, 86.5%, 92.3%

As a concentration of 1.0mg/mL of lotus wheat drink solution, clearance rate 42.2l%, and the clearance rate of same concentrations Vc is 45.39%, the IC of linear the Fitting Calculation get He Mai drinks and Vc₅₀Respectively 1.429mg/mL and 1.098mg/mL.It can be seen that lotus wheat Kitchenware has the stronger ability for removing hydroxyl radical free radical, and gradually increasing with lotus wheat drink mass concentration, to hydroxyl radical free radical Scavenging activity also enhance therewith, good dose-effect relationship is presented, but elimination effect is approached with Vc.

4.3 remove the measure of ultra-oxygen anion free radical ability

Ultra-oxygen anion free radical is first free radical in all oxygen radicals, can pass through series reaction and generate Other oxygen radicals have a close ties with many diseases, and the removing of ultra-oxygen anion free radical is for protecting morning of body cell Phase oxidative damage is most important.Using the scavenging effect of assay NBT photoreduction research ultra-oxygen anion free radical.Take 0.4, 0.8th, 1.2,1.6, the lotus wheat drink solution of 2.0mg/mL various concentrations, surveys absorbance, V1 is control tube neighbour benzene three in 325nm wavelength The white oxidation rate of phenol, △ A/min；Autoxidation rates of the V2 for sample cell pyrogallol, △ A/min.With 0.02, O.04, 0.06th, 0.08, the Vc of 0.10mg/mL various concentration gradients does positive control.Calculate the clearance rate to ultra-oxygen anion free radical. Clearance rate (%)=(mono- V2 of V1)/Vl × 100

When lotus wheat drink concentration reaches 2.0mg/mL, clearance rate 32.2l%, and Vc is removed in a concentration of 0.1mg/mL Rate after increase lotus wheat drink solution concentration, has the tendency that enhancing up to 45.28% to the Scavenging activity of ultra-oxygen anion free radical, Show dose-effect relationship.The IC of linear the Fitting Calculation get He Mai drinks and Vc₅₀Respectively 2.891mg/mL and 0.144mg/mL, Lotus wheat drink has certain scavenging effect to the superoxide anion that mouse thymus cells system generates.

The measure of 4.4 reducing powers

Take 0.2,0.4,0.6,0.8, the lotus wheat drink solution 1mL of 1.0mg/mL various concentrations, in 10mL centrifuge tubes respectively The phosphate buffer 2.5mL of 0.2mol/L pH6.6 is added in, adds in 1% potassium ferricyanide solutions of 2.5mL, is uniformly mixed after 50 DEG C reaction 20min.10% trichloroacetic acids of 2.5mL are added in after taking-up and terminate reaction, 5000r/min centrifugations 10min.Take supernatant 2.5mL adds people 2.5mL distilled water and 0.5mL liquor ferri trichloridis, 10min is stood after mixing, and light absorption value is detected at 700nm. Using 0.02,0.04,0.06,0.08, the Vc of 0.10mg/mL various concentrations as positive control, measured under the conditions of wavelength 700nm Absorbance.The results show that with the increase of polysaccharide concentration, absorbance value gradually increases, and represents that the reducing power of sample is stronger.In phase With under the conditions of, the absorbance of 0.2mg/mL Vc reaction systems at 700nm is 1.139, and the lotus wheat of 1.0mg/mL drink solution Absorbance is 0.374.This shows that lotus wheat drink solution can provide electronics, and combined with free radical, is converted to stable substance, from And the generation of free radical is terminated, there is certain reducing power, reducing power increases with the increase of sample concentration, in amount Effect relationship.

Claims (3)

1. a kind of lotus wheat drinks health care particle, which is characterized in that adds in addition after ultramicro grinding by the component of following portions by weight Agent mixing granulation forms：5-10 parts of lotus leaf, 4-8 parts of barley seedling, 4-8 parts of corn stigma, 3-6 parts of mulberry leaf, 3-6 parts of gynostemma pentaphylla.

2. lotus wheat as described in claim 1 drinks health care particle, which is characterized in that by the component of following portions by weight through Ultramicro-powder Broken, mixing granulation forms：6-9 parts of lotus leaf, 5-8 parts of barley seedling, 5-8 parts of corn stigma, 4-5 parts of mulberry leaf, 4-5 parts of gynostemma pentaphylla.

3. lotus wheat as claimed in claim 1 or 2 drinks health care particle, which is characterized in that preparation method is:First by each component point It is not crushed to less than 10 μm nanoscale Ultramicro-powders, then adds in after additive is uniformly mixed and squeezes granulation into particle through dry method Agent.