KR20000056324A - Freeze dried retinol microsphere and a lip-care cosmetic composition containing the same - Google Patents

Abstract

PURPOSE: Lyophilized retinol microsphere and lip cosmetics containing the microsphere are provided, to remove the lip wrinkle. CONSTITUTION: A lyophilized retinol microsphere is prepared by adding tri(capryl, caprilic acid)glycerin to the solution of athero-collagen and sodium chondroitin sulfate, stirring it, and passing it through a microfluidizer; adding retinol and tocopherol to the mixture, stirring it, and passing it through a microfluidizer; and adding acacia (gum arabic) to the mixture, and lyophilizing it at the temperature of -40°C. Lip cosmetics composition comprises 2,000-6,000 IU of the lyophilized retinol microsphere per 1 g; and optionally 0.5-3 wt% of lyophilized collagen microsphere. The lyophilized collagen microsphere is prepared by adding tri(capryl, caprilic acid)glycerin to the solution of athero-collagen and sodium chondroitin sulfate; stirring it; passing it through a microfluidizer; lyophilizing it; and adding it hydrophobic gel to homogenize it.

Description

Freeze dried retinol microspheres and a lip-care cosmetic composition containing the same

The present invention relates to a retinol-containing lip cosmetic composition, and more particularly, to a lyophilized retinol microspheres having freeze-dried microspheres formed by coating retinol with collagen, thereby improving the storage stability of retinol and the lips by containing them. It relates to a lip cosmetic composition that can provide an anti-wrinkle effect.

Retinol, retinic acid, retinaldehyde and derivatives thereof are spotlighted for their excellent anti-wrinkle effect, and high-functional cosmetics formulated with cosmetics are gaining popularity.

However, since the retinoid compounds are easily oxidized by oxygen in the air as well as moisture in the cosmetic base, as compared to other cosmetic active ingredients, the retinoid compounds lose their activity. In this case, the retinol titer does not appear.

Accordingly, in the cosmetic field, researches on various encapsulation for isolating retinol from moisture-containing cosmetic bases have been actively conducted to secure material stability of retinol.

For example, Korean Patent Application Publication No. 1998-21511 proposes a double capsule encapsulating a second matrix encapsulated with gellan gum with micro-sized particles primary encapsulated with retinol as an internal wound, and Korean Patent Application Publication No. 1998-703668 Has proposed a skin protective composition containing encapsulated retinol in nonphospholipid liposomes.

On the other hand, the present inventors have been focused on the development of cosmetics for protecting the skin of the lips, while it is expected to be able to effectively improve the wrinkles of the lips by containing retinol has been studied to formulate retinol as a lip cosmetics.

In the case of the lip cosmetics, the moisture content is almost zero compared to other cosmetics, but considering the oxidation by the fatty acid-based component or the metallic pigment component in the base, retinol encapsulation is required as in the conventional skin care cosmetics. do.

In the encapsulation of retinol, the present inventors have completed the present invention by encapsulating retinol in collagen and lyophilizing it to maximize the material stability of retinol, and also provide skin moisturizing effect.

Accordingly, it is an object of the present invention to provide a lyophilized retinol microspheres that can maximize the material stability of retinol in a lip cosmetic base.

Another object of the present invention is to provide a lip cosmetic composition which can obtain a lip wrinkle improvement effect by containing the lyophilized retinol microspheres.

The above and other objects of the present invention will become apparent from the following detailed description of the invention.

1 is a cross-sectional view of a multi-molding machine for producing a dual structured lipstick of the present invention.

In order to achieve the above object, the lyophilized retinol microspheres according to the present invention,

(1) adding tri (capryl, capric acid) glycerin to the atherocollagen and chondroitin sulfate sodium solution and stirring it sufficiently, and then passing it through a microfluidizer;

(2) adding retinol and tocopherol to (1) and stirring, and then passing the microfluidizer again; And

(3) adding acacia gum to (2) and freeze-drying at -40 ° C

It is manufactured by a process comprising a.

The lyophilized retinol microspheres thus prepared are maintained at least 99% of the retinol titer at the time of manufacture, even after 6 months, as confirmed in the test example described below, even when formulated in the lip cosmetics 96% or more It can be seen that the material stability of the retinol is greatly improved.

Lip cosmetic composition according to an embodiment of the present invention is characterized in that it contains the lyophilized retinol microspheres in an amount of 2,000 ~ 6,000 IU per gram of the composition.

Furthermore, the collagen microspheres obtained in step (1) of the above manufacturing process are lyophilized and added to a hydrophobic gel composed of polyglyceryl-6-diolate, epoxy diglycol, propylene glycol laurate, and the like. And further comprising lyophilized collagen microspheres obtained by homogenization.

As can be seen in the test examples described later, by containing the lyophilized retinol microspheres and the lyophilized collagen microspheres at the same time, compared to the case of using lyophilized retinol microspheres alone, Reducing the content can achieve the same degree of lip wrinkle improvement.

Lip wrinkle improvement effect of lyophilized collagen microspheres is because collagen microspheres swell in the process of capturing and rehydrating moisture loss evaporated from percutaneous skin, causing swelling of skin and improving surface wrinkles. Interpreted In addition, it is preferable to contain the lyophilized collagen microspheres, so that transdermal moisture loss can be suppressed and the skin moisturizing effect can also be obtained.

Therefore, the lip cosmetic composition according to another embodiment of the present invention is characterized in that it further contains the lyophilized collagen microspheres in an amount of 0.5 to 3% by weight of the total weight of the composition.

Lip cosmetic composition according to the embodiments of the present invention described above can provide an effective cosmetic effect to deep wrinkles and severe lips through the skin swelling effect through the wrinkle improvement effect and moisture absorption of retinol and collagen.

Hereinafter, the other components constituting the lip cosmetic composition of the present invention will be described.

Lip cosmetic composition according to the present invention, 9 to 25% by weight of solid oil-based base, 25 to 45% by weight of high viscosity oil-based base, 10 to 35% by weight of low viscosity oil-based base, 1 to 15% by weight pigment, freezing 0.5-3% by weight of dry collagen microspheres and lyophilized retinol microspheres are contained in an amount of 2,000-6,000 IU per gram of the composition.

The solid oil-based base used in the present invention means an oil-based substance that maintains a solid at a sample temperature of 25 ± 0.5 ° C. and cannot measure viscosity in rotary Brookfield viscometers (LVT, RVT), For example, carnauba lead, candelilla lead, microcrystalline lead, ceresin lead, cetyl alcohol, synthetic wax and the like are good examples, but are not limited thereto.

In addition, the high viscosity oil base means an oily substance having a viscosity of 200 cps or more as measured by a rotary Brookfield viscometer (RVT) at a sample temperature of 25 ± 0.5 ° C., for example, lanolin (48,000 cps), castor oil (300 cps), Liquid lanolin (916 cps), polybutene having an average molecular weight of 500 to 2,500, petrolatum (102,000 cps) and the like are good examples, but are not limited thereto.

In addition, a low viscosity oily base means an oily substance having a viscosity of less than 200 cps measured by a rotary Brookfield viscometer (LVT) at a sample temperature of 25 ± 0.5 ° C., for example, liquid paraffin (22 cps), squalane (35 cps) , Isopropyl palmitate (25 cps), isopropyl myristate (25 cps), cetyl lactate (22 cps), octyl dodecyl myristate (25 cps), octyl dodecanol (38 cps), olive oil (46 cps), polydecine (138 cps) ), Caprylic / capric triglyceride (27 cps) and the like are good examples, but are not limited thereto.

Such a solid, high viscosity, low viscosity oily base can be used 1 type or in combination of 2 or more types.

In addition, pigments having a color can be used without limitation organic and inorganic pigments that have been commonly used in lip cosmetic compositions, for example, red pigments 202, red 223, red 201, statutory pigments as organic pigments, One or two micas coated with titanium dioxide, zinc oxide, talc, kaolin, ferric oxide, ferrous trioxide, and titanium oxide are used as inorganic pigments such as red 218, red 227, orange 201, yellow 5, and the like. It can be used in combination of two or more kinds.

In the case of a lipstick having a color by containing such a pigment component, a color structure of a tinted color and a portion containing a cosmetic component are separated and formed in a dual structure to prevent oxidation of the cosmetic component by the pigment component. It is possible to increase the consumer's desire to buy by visualizing the portion containing the cosmetic ingredients.

The dual structured lipstick consists of a cosmetic central axis containing cosmetic ingredients and a color body containing pigment ingredients surrounding the cosmetic ingredients.

Specifically, the cosmetic central axis is 15 to 25% by weight solid oil base, 35 to 45% by weight high viscosity oil base, 25 to 35% by weight low viscosity oil base, 0 to 5% by weight pigment based on the total weight of the central axis As a component, lyophilized collagen microspheres are formulated to contain 0.5 to 3% by weight relative to the total weight of the composition, and lyophilized retinol microspheres in an amount of 2,000 to 6,000 IU per gram of the composition; In addition, the color body is composed of 15 to 23% by weight of solid oil base, 40 to 45% by weight of high viscosity oil base, 20 to 30% by weight of low viscosity oil base, and 10 to 15% by weight pigment based on the total weight of the color body. do. The dual structured lipstick may be configured by bonding a central axis containing a cosmetic ingredient and a color body having a color tone at a weight ratio of 3: 7 to 4: 6. The dual structured lipstick of this configuration is illustrated in Example 4 and can be prepared using a multi-molding machine illustrated in schematic cross-sectional view in FIG.

Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited to these Examples.

Preparation Example 1 Preparation of Lyophilized Retinol Microspheres:

Aterocollagen and sodium chondroitin sulfate solution was simply stirred at 40-45 degreeC by the ratio of 7: 3. 1 g of the obtained liquid mixture was mixed with 58 g of tri (capryl, capric acid) glycerin, sufficiently stirred, and encapsulated by passing through a microfluidizer, and then cooled to 25 ° C.

15 g of retinol and 1 g of tocopherol were added to the encapsulated microspheres at room temperature, and then stirred. Then, the retinol and tocopherol were encapsulated by passing through a microfluidizer. 25 g of acacia gum was added to the encapsulated contents, and the mixture was sufficiently mixed and lyophilized at -40 ° C.

Preparation Example 2 Preparation of Lyophilized Collagen Microspheres:

Aterocollagen and sodium chondroitin sulfate solution was simply stirred at 40-45 degreeC by the ratio of 7: 3. 1 g of the obtained liquid mixture was mixed with 58 g of tri (capryl, capric acid) glycerin, sufficiently stirred, and encapsulated by passing through a microfluidizer, and then cooled to 25 ° C. Encapsulated collagen microspheres were lyophilized at -40 ° C.

60 g of polyglyceryl-6-dioleate, 20 g of ethoxydiglycol, and 17 g of propylene glycol laurate were homogenized by stirring at 70 ° C, and then cooled to room temperature. It was homogenized by adding 3 g of the lyophilized collagen microspheres obtained above.

<Examples 1-3 and Comparative Examples 1-3> (Single structured lipstick)

content(%) Example Comparative example One 2 3 One 2 3 1 2 3 4 5 Candelilla lead carnauba lead microcrystallin lead beeswax ceresin 8.00 1.00 3.00 3.00 6.00 Solid Mechanism 678 Lanolin Polybutene Diisostearyl Maleate to 100 3.0013.00 High Viscosity Oily Mechanism 9101112 Isononyl isononanoate phenyl trimethicone polydecine caprylic / capric triglyceride 8.40 7.0010.00 2.00 Low Viscosity Oil Based Mechanism 131415161718 Talc Black Iron Oxide Ferric Oxide Yellow No. 5 Red No. 202 Titanium Dioxide 2.00 1.00 0.70 8.00 0.10 2.40 Pigment 1920212223 Retinol (250,000 IU / g) Globules (Preparation Example 1) Collagen Microspheres (Preparation Example 1) Preservative Antioxidant Flavor 1.201.000.200.200.20 1.20-0.200.200.20 2.20-0.200.200.20 --0.200.200.20 -1.001.000.200.20 -2.000.200.200.20

<Production method>

Raw materials # 1-12, # 21-22 were weighed and homogenized by heating to 85-90 DEG C in a water bath, and then pigments # 13-18 were added to prepare a dispersion operation by simple stirring.

Dispersion process was performed five times using a three-stage roll mill, then heated to 80 ~ 85 ℃ again to melt and homogenized by adding raw materials # 19 ~ 20, 23, and then prepared by molding through a defoaming and filtration process It was.

After molding ① clean the molding machine cleanly, prepare the contents (Examples and Comparative Examples) in the molten state, ② inject the melted contents into the molding machine, put into the cooler, ③ the surface temperature of the molding machine 6 ~ 8 When it is lowered to ℃, take out the molding machine, and ④ insert the contents into the container to make the final sample.

<Example 4 and Comparative Example 4> (double structured lipstick)

ingredient <Color body> content (%) <Beauty center axis> content (%) Remarks Reference Example Example 4-core Comparative Example 4-core 123456 Candelilla lead carnauba lead microcrystalline lead beeswax ceresin ozokerite lead 8.00 1.00 3.00 3.00 6.00- 8.00-3.00 1.00 4.00 1.00 Solid Mechanism 78910 Lanolin polybutene diisostearyl maleate methyl polysiloxane 20.00 3.0012.80- 3.00 3.00to 10020.50 High Viscosity Oily Mechanism 1112131415 Phenyl Trimethicone Polydecine Caprylic / Capric Triglyceride Liquid Paraffin Iononyl Isononanoate 7.0010.00 2.00- 8.40 --- 20.50- --- 20.50- Low Viscosity Oil Based Mechanism 161718192021 Talc Black Iron Oxide Ferric Oxide Yellow No. 5 Red No. 202 Titanium Dioxide 2.001.000.709.000.102.40 ------ ------ Pigment 2223242526 Retinol (250,000 IU / g) globules (manufacturing example 1) collagen globules (manufacturing example 2) antiseptic antioxidant fragrance --0.200.200.20 3.502.500.200.200.20 --0.200.200.20 Combination 60% by weight 40 wt%

<Production method>

〈1〉 Color Body

The raw materials # 1-6 were warmed to 85-90 ° C. and completely melted, and then # 7-10, # 12-14 and # 24-25 were added thereto, followed by simple stirring for complete melting.

Thereafter, raw materials # 16 to 21 were added thereto, followed by simple stirring, followed by homogeneous dispersion treatment five times using a three-stage roll mill. The mixture was warmed to 80 to 85 ° C. to be melted, and then homogenized by adding raw material # 26, and aged after undergoing defoaming and filtration at a constant temperature of 80 to 85 ° C.

〈2〉 Beauty Center

Raw materials # 10, 14 were mixed and homogeneously dispersed at room temperature using a T. K. Robo mixer (speed: 2000 rpm. Time: 10-15 minutes). The raw materials # 1 and # 3-9 which were heated at 85-90 degreeC and were fully melt | dissolved were added here, and it stirred simply. Thereafter, raw materials # 24 and 25 were added thereto, followed by melting by simple stirring.

Once completely dissolved, raw materials # 22-23 and 26 were added and treated at 2,500 rpm for 10 minutes using a T. K. Robo mixer at 80 ° C, followed by defoaming to mature at room temperature.

〈3〉 Forming method:

① After cleaning the inside of the multiple molding machine of FIG. 1, the molding machines a, b, and c are set.

Prepare the colored body of <1> and the beauty center axis content of <2> by heating and melting at 80-85 degreeC.

② Inject molten color body of <1> into molding machine.

③ Put the molding machine into the cooler and when the surface temperature is lowered to 8 ~ 12 ℃, take out the molding machine and remove a.

④ Inject the molten cosmetic core content of <2> into the space from the molding machine a.

⑤ After injecting the contents of the cosmetic core, put the molding machine into the cooler and cool it again.

⑥ After about 10 ~ 15 minutes after inserting the cooler, take out the molding machine and remove the molding machine b and insert it into the container.

This completes the dual purpose lipstick that is the final goal.

<Example 5> Lip Gloss

ingredient Example 5 Remarks 123 Carnauba Lead Ozokerite Lead Microcrystalline Lead 2.005.002.00 Solid Planetary Mechanism 45 Lanolin Polybutene 40.00to 100 High Viscosity Planetary Mechanism 6 Floating paraffin 8.00 Low Viscosity Planetary Mechanism 7 Red 202 2.00 Pigment 89 Retinol (250,000 IU / g) globules (manufacturing example 1) collagen globules (manufacturing example 2) 1.201.00 101112 Preservative Antioxidant Flavor 0.200.200.20

<Production method>

Melt and homogenize raw materials # 1 ~ 6, 10, 11 by heating to 85 ~ 90 ℃, process # 5 with a three-stage roll mill, and then melt by heating to 80 ~ 85 ℃. After homogenization by adding # 8, 9 and 12, the molding was prepared by degassing and filtering.

Molding is carried out by injecting the contents of 80 ℃ in a square shell (5 x 2.5 cm), and then cooled to room temperature to complete the molding and aged.

<Test Example 1> Lip skin moisture protection and control function confirmation test (single structured lipstick):

Example 1 and Comparative Example 1 Lip skin moisture protection function for the lipstick product was evaluated by conducting a clinical confirmation test.

Specifically, a female of 20 to 50 years old, each of the owners of normal lips and ten owners of lips with severe wrinkles and keratin peels, respectively. The lipstick of 1 was applied to the lip skin after washing every morning, and after 1 hour, 2 hours, and 3 hours, TransEpidermal Water Loss was measured using a transdermal water evaporation meter (TEWAMETER ^™ TM210). The results are shown in Table 1.

Example 1 lipstick Comparative Example 1 Lipstick T ₀ T ₁ T ₂ T ₃ T ₀ T ₁ T ₂ T ₃ TEWL (mg / cm 2 / hr) Normal lip 0.454 ± 0.087 0.228 ± 0.086 0.239 ± 0.090 0.250 ± 0.080 0.454 ± 0.087 0.378 ± 0.090 0.390 ± 0.081 0.398 ± 0.080 Wrinkled lips 0.545 ± 0.091 0.255 ± 0.087 0.270 ± 0.090 0.299 ± 0.065 0.545 ± 0.091 0.495 ± 0.095 0.500 ± 0.090 0.505 ± 0.068 Average (mg / cm 2 / hr) 0.499 ± 0.089 0.271 ± 0.086 0.241 ± 0.088 0.275 ± 0.072 0.499 ± 0.089 0.437 ± 0.093 0.445 ± 0.086 0.451 ± 0.074 (Note) T ₀ : before use, T ₁ : 1 hour after use, T ₂ : 2 hours after use, T ₃ : 3 hours after use

From the results of Table 1, it can be seen that the lipstick of Example 1 containing the lyophilized retinol microspheres and the lyophilized collagen microspheres was more effective in reducing the amount of transdermal water loss than the lipstick of Comparative Example 1 not added.

<Test Example 2> Lip wrinkle improvement effect confirmation test (single structure lipstick):

Lip wrinkle improvement effect on the lipstick of Examples 1-3 and Comparative Examples 1-3 was evaluated by performing a clinical confirmation test.

Specifically, a female group of 20-50 years old, divided into three groups of 30 owners of normal lips and 30 owners of highly wrinkled lips, compared the lipstick of the embodiment to the left lips and the right lips to the same person. Example lipstick was applied three times a day (three hours apart) after washing every morning, and changes in the wrinkle area and number of lips were measured by skin three-dimensional analysis (Image Analyser nexusQube ^™ ). The results are shown in Table 2.

Changes in lip wrinkles Wrinkle count reduction rate Wrinkle area reduction rate Remarks Normal lips Wrinkled severe lips Normal lips Wrinkled severe lips Example 1 -16% -29% -34% -40% Retinol & Collagen Example 2 -10% -19% -22% -29% Retinol 1.2% Example 3 -14% -31% -31% -42% Retinol 2.2% Comparative Example 1 -One % -3% -2 % -5% - Comparative Example 2 -5% -8 % -7% -12% Collagen 1.0% Comparative Example 3 -8 % -11% -9% -14% Collagen 2.0%

From the results in Table 2, it can be seen that the lipstick of Example 1 containing the lyophilized retinol microspheres and the lyophilized collagen microspheres has a much better wrinkle improvement effect than the lipstick of Comparative Example 1 not added.

Also, by comparing the lipstick of Example 1 with the lipstick of Example 3, compared to the case of containing only lyophilized retinol microspheres, the same wrinkles were contained by containing less amount of retinol simultaneously by simultaneously containing lyophilized collagen microspheres. It can be seen that an improvement effect can be obtained.

Test Example 3 Lip Skin Moisture Protection and Control Function Confirmation Test (Double Structural Lipstick):

Lip skin moisture protection function of the dual structured lipstick product of Example 4 (Reference Example + Example 4-core) and Comparative Example 4 (Reference Example + Comparative Example 4-core) was evaluated by conducting a clinical confirmation test.

Specifically, it carried out in the same manner as in Test Example 1, the results are shown in Table 3.

Example 4 Lipstick Comparative Example 4 Lipstick T ₀ T ₁ T ₂ T ₃ T ₀ T ₁ T ₂ T ₃ TEWL (mg / cm 2 / hr) Normal lip 0.454 ± 0.087 0.208 ± 0.085 0.219 ± 0.086 0.200 ± 0.086 0.454 ± 0.087 0.362 ± 0.090 0.383 ± 0.081 0.350 ± 0.076 Wrinkled lips 0.545 ± 0.091 0.250 ± 0.087 0.260 ± 0.090 0.290 ± 0.065 0.545 ± 0.091 0.490 ± 0.087 0.504 ± 0.091 0.510 ± 0.068 Average (mg / cm 2 / hr) 0.499 ± 0.089 0.299 ± 0.086 0.239 ± 0.088 0.245 ± 0.070 0.499 ± 0.089 0.426 ± 0.088 0.435 ± 0.086 0.430 ± 0.072 (Note) T ₀ : before use, T ₁ : 1 hour after use, T ₂ : 2 hours after use, T ₃ : 3 hours after use

From the results in Table 3, it can be seen that the lipstick of Example 4 containing the lyophilized retinol microspheres and the lyophilized collagen microspheres was more effective in reducing the amount of transdermal water loss than the lipstick of Comparative Example 4 not added.

<Test Example 4> Lip wrinkle improvement effect confirmation test (single structure lipstick):

Lip wrinkle improvement effects on the lipstick of Example 4 and Comparative Example 4 were evaluated by conducting a clinical confirmation test.

Specifically, the subjects were 20 to 50 years old and each of the owners of the normal lips and the owners of the lips with severe wrinkles were subjected to the same method as in Test Example 2, and the results are shown in Table 4.

Changes in lip wrinkles Wrinkle count reduction rate Wrinkle area reduction rate Normal lips Wrinkled severe lips Normal lips Wrinkled severe lips Example 4 -15% -27% -31% -38% Comparative Example 4 -One % -2 % -3% -5%

From the results of Table 4, it can be seen that the lipstick of Example 4 containing the lyophilized retinol microspheres and the lyophilized collagen microspheres had a much better wrinkle improvement effect than the lipstick of Comparative Example 4 not added.

Test Example 5 Retinol Titer Determination:

Preservation stability was evaluated by measuring the titer over time of the lyophilized retinol microspheres used in Example 1 and the retinol contained in Example 1. Retinol titer measurements were analyzed by HPLC. HPLC analysis conditions were made using a Shimpack CLC-ODS column, and the mobile phase was methanol, the wavelength was 284 nm, and the flow rate was 2.0 ml.

The results are shown in Table 5.

Sample name Retinol Titers Analyzed (IU / g) After 3 months After 6 months Preparation Example 1 (Retinol 250,000 IU / g) 248,625 (99.45% of displayed titres) 247,750 (99.65% after reorganization) 247,250 (99.45% after reorganization) Example 1 (Retinol 3,000 IU / g) 2,470 (82.33% of displayed titres) 2,408 (97.49% after post-organization) 2,380 (96.36% after reorganization)

From the results in Table 5, although there is a slight decrease in the titer of retinol at the time of manufacture, it can be seen that there is almost no decrease in the titer of retinol over time.

The lyophilized retinol microspheres provided by the present invention maintain at least 99% of the retinol titer at the time of manufacture even after six months, and at least 96% of the titer of the retinol even when formulated in the lip cosmetics. It is very effective, and by blending it with the lip cosmetics, it is possible to effectively improve the wrinkles of the lips.

Claims (8)

(1) adding tri (capryl, capric acid) glycerin to the atherocollagen and chondroitin sulfate sodium solution and stirring it sufficiently, and then passing it through a microfluidizer; (2) adding retinol and tocopherol to (1) and stirring, and then passing the microfluidizer again; And (3) adding acacia gum to (2) and freeze-drying at -40 ° C Method for producing a lyophilized retinol microspheres comprising a. Lyophilized retinol microspheres obtained by the method of claim 1. A lip cosmetic composition comprising the lyophilized retinol microspheres according to claim 2 in an amount of 2,000 to 6,000 IU per gram of the composition as a cosmetic ingredient. The method of claim 3, wherein tri (capryl, capric acid) glycerin is added to the solution of atherocollagen and sodium chondroitin sulfate, and the mixture is sufficiently stirred, passed through a microfluidizer, lyophilized, and then added to a hydrophobic gel. A lip cosmetic composition comprising lyophilized collagen microspheres obtained by homogenization in an amount of 0.5 to 3% by weight based on the total weight of the composition as a cosmetic ingredient. 5. A dual structured lipstick according to claim 3 or 4, comprising a cosmetic central axis containing a mixture with lyophilized retinol microspheres or lyophilized collagen microspheres as a cosmetic ingredient, and a color body having a color tone surrounding the cosmetic constituents. Lip cosmetic composition, characterized in that it has a formulation. The lip cosmetic composition according to claim 5, wherein the dual structured lipstick is formed by bonding a central axis containing a cosmetic ingredient and a color body having a color tone at a weight ratio of 3: 7 to 4: 6. According to claim 6, wherein the cosmetic central axis is 15 to 25% by weight solid oil base, 35 to 45% by weight high viscosity oil base, 25 to 35% by weight low viscosity oil base, pigment 0-5 weight based on the total weight of the central axis %, And a cosmetic component comprising lyophilized collagen microspheres in an amount of 0.5 to 3% by weight based on the total weight of the composition and lyophilized retinol microspheres in an amount of 2,000 to 6,000 IU per gram of the composition; The above-mentioned color body is composed of 15 to 23% by weight of solid oil base, 40 to 45% by weight of high viscosity oil base, 20 to 30% by weight of low viscosity oil base, and 10 to 15% by weight pigment based on the total weight of the color body. Lip cosmetic composition, characterized in that. Freeze-dried collagen obtained by adding tri (capryl, capric acid) glycerin to the solution of atherocollagen and sodium chondroitin sulfate, stirring it sufficiently, passing through a microfluidizer, lyophilizing, and then adding it to a hydrophobic gel and homogenizing it. Microspheres.