KR20000037233A - New Disinfectant and Antibacterial Compounds (Polyiodideanionresins) for Aquaculture - Google Patents

Abstract

PURPOSE: A multi iodine anion resin is used for manufacturing a sterilizing and antibacterial substance which is applied to prevention and treatment of diseases and harmless to the human body. Thereby, public health is improved. CONSTITUTION: Iodine(12) and iodine anion(1-) are mixed in the certain molar ratio to form 13-, 15-, 17- iodine anion and these are absorbed into anion exchange resin(Dowex etc.) to produce iodine anion resin which can be used instead of antibiotics for fish farm. The iodine anion resin can be also used for wash Bidet, humidifier or air conditioner.

Description

New aquaculture disinfection and antibacterial substances; Multi-iodine Resin {New Disinfectant and Antibacterial Compounds (Polyiodideanionresins) for Aquaculture}

In recent years, fish farms have increased resistance to pathogens, resulting in difficulty in reducing the effectiveness of drugs even when they are actually administered. In addition, the use of drugs in fishery aquatic use of the remaining drugs in fish is causing problems that are ingested in humans. In fact, the antibiotics used in farming are: Vibrio disease, which occurs in June-August during defense, is used by administering tetracycline antibiotics at 50mg / kg body weight per day for 5 consecutive days. Streptococcus, which usually occurs in June-August In the treatment of symptoms, antibiotics such as erythromycin, oxytetracycline hydrochloride, embonic acid spiramycin, and chitasamycin are administered. In the case of halibut, Vibrio disease is characterized by black body color in March-October, collapse of body surface and ulceration. Antibiotics such as oxytetracycline, chloramphenicol and neomycin are used. Streptococcosis, a symptom of mucus secretion, red lips and fins, and intestinal bleeding on the gills, gills and body surface, is treated with erythromycin, spiramycin and probemycin for 1 to 2 weeks. In December, Edward's disease, which causes color fading, bloating, and hernias in the anus, reddening, and without feeding, floating forcelessly on the edge of the tank, is followed by continuous administration of antibiotics such as oxytetracycline and theramycin for about two weeks. Healing The above problems are difficult to solve with antibiotics such as tetracycline.

The technical problem to be achieved by the present invention is to apply a multi-iodine anion resin to prevent and treat various diseases of the aquaculture industry, and its efficacy was verified using the multi-iodine anion resin manufactured by the inventors. In addition, the only substance that can be absorbed by fish after use of this substitute is iodine, which is an essential element and must be supplied to humans. In developed countries, it is added to salt to supplement it. Rather, it has the effect of supplementing essential substances. In addition, the present invention is proven to be effective as a fungicide, such as a large air conditioner, a humidifier, a large air conditioner, cooling water, etc., can be used to broaden its application. In particular, the use of antibiotics in the most important aquaculture industry of this patent will lead to a breakthrough in domestic aquaculture technology as a method that is not harmful, cumulative and non-resistant.

The sterilization power of the multi-iodine anion resin will vary according to the water environment and conditions, and the sterilization power will be changed depending on the pH, organic matter concentration, salt and temperature. Therefore, these optimum conditions of the polyiodine anion resin produced by the inventors were investigated.

① Sterilization

Sterilization of multi-iodine anion resin was filled with 3.8 g of i-iodine resin in a column (glass 2 cm in diameter and 10 cm in length), and then passed through the water at 20 mL / min. Checked to find out. The results at this time are shown in Table 1 below. As a result, most prepared microorganisms were sterilized when they passed through the resin.

〈Table 1〉 Sterilization effect of iodide ion resin

Microorganism types Concentration (CFU / ml) Resin pass After the resin passed Bacillus spp. 1.1 × 10 ³ 0 Escherichia coli 1.2 × 10 ³ 0 Pseudomonas aeruginosa 1.1 × 10 ³ 0 Staphylococcus aureus 1.1 × 10 ³ 0 Salmonella typhimurium 1.0 × 10 ⁶ 0 Streptococcus fecalis 1.1 × 10 ⁴ 0

The bactericidal power was very good even when the water prepared with microorganisms was poured into the container while the iodine resin was immersed in the bottom of the container. Experiments showed that 50 g of Diodine anion resin was immersed in a 20 L large glass container and then completely killed after 30 minutes when the microorganism of Table 1 was added to about 10 L of water prepared at 1,000 CFU / mL. . However, at a concentration of 100,000 CFU / mL, the bactericidal power was about 80% within 30 minutes. At this time, the same effect was obtained when stirring or increasing the amount of iodide ion resin used.

Opening and releasing stoppers of drinking spring water can cause general bacteria to increase. When the five kinds of drinking water were opened with the lid open and stored at room temperature for 15 days, the rate of increase of general bacteria and the rate of growth of general bacteria after immersion in 5 g / 10 L of Dyodine anion resin were immersed in one drinking water. Is shown in <Table 2>. As can be seen from the results, the common bacteria did not grow in the drinking spring immersed in the DIA.

〈Table 2〉 General bacterial growth degree of drinking spring water and the effect of inhibiting bacterial growth of multi-iodine anion resin over time (Unit: CFU / mL)

Type of water Early 3 days later 5 days later 7 days later 10 days later 15 days later A 0 0 0 0 15 32 B 0 0 0 52 102 230 C One 8 170 180 250 410 D One 9 210 400 510 960 E 0 4 190 190 280 530 Multi-iodine Resin + E 0 0 0 0 0 0

From the above results, pathogenic microorganisms can be sterilized simply by immersing the water in the water or by passing through the column filled with the water, and by immersing the water in water, no new microorganisms are produced. Able to know.

② Microbial sterilization effect according to pH, temperature and organic concentration

The microbial sterilization effect according to pH was investigated. The sterilizing power of each resin concentration was examined by passing 5 L of water prepared by four strains through a column filled with 5 g of a multi-iodine resin. Changes in pH were slightly acidic (6), neutral (7), and weakly alkaline (8), resulting in 100 Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis 100 / mL, 1,100 / mL, 12,000 / mL and 100,000 / mL was passed through the resin. Experimental results showed that the most effective bactericidal power was pH7, and the bactericidal power was decreased in weak acid and weak alkali. Therefore, in order to have maximum bactericidal power, pH should be adjusted to neutral.

The microbial sterilization effect with temperature change was investigated. At this time, the temperature change was selected from 10, 30, 70 ℃, and the species was used Staphylococcus aureus, and the Dayo anion resin was filled with a column of 5g at a height of 3cm and passed through 10L at a flow rate of 2.4L / min. It was. As a result of the experiment, it was observed that 100% uniform sterilization power was maintained at a temperature of 10 ° C. or higher and 70 ° C. or lower. However, when the temperature is high, the iodine is eluted from the resin a lot, the appropriate temperature is 10 ~ 30 ℃.

〈Table 3〉 Sterilization effect of multi-iodine anion resin by temperature change (Unit: CFU / mL)

Temperature condition (℃) Pre-pass concentration (n = 5) Concentration after passage (n = 5) 10 1100 0 30 1000 0 70 1000 0

The bactericidal effect according to the organic concentration was investigated. The presence of organic matter in the water vapor can interfere with the contact of the microorganisms with the iodide resin and consequently reduce the bactericidal power. The experimental results are shown in Table 4, where the sterilizing power was correlated with COD concentration of = 0.99. In other words, in order to have maximum sterilization power of the polyhydric anion resin, the concentration of organic matter should be lowered as much as possible.

<Table 4> Sterilization according to organic concentration

COD concentration General bacteria Escherichia coli (mg / L) Before passing After passing % Removal Before passing After passing % Removal 7.2 5,200 140 97.3 68 <One 99.99 8.2 5,000 340 93.2 59 3 94.9 13.6 5,100 1,953 61.7 60 14 76.7 14.6 4,800 2,097 56.3 61 18 70.5 19.0 5,000 3,355 32.9 58 28 51.7

* The preparation water is passed through 5L at a flow rate of 2.4L / min through a 3cm thick resin

From the above results, in order to properly use the polyhydric anion resin, the pH should be adjusted to neutral, and the temperature is preferably 10 to 30 ° C, and it is preferable to minimize the organic content.

③ Safety and Lifespan

Even though the iodine resin has excellent sterilizing power, if the substance eluted from it is harmful or accumulative, its use value is low. Therefore, to find out the substance eluted after the use of the iodine resin, all items and standards of drinking water quality standards The compound was investigated. At this time, the water was passed through the column uniformly filled with 4 cm thick resin to analyze the water quality before and after passage. The amount of water passed was 1000L, 5000L, 10000L, 20000L and the drinking water quality standard items were investigated. As a result, no harmful substances under the drinking water quality criteria were detected, and I-elution was less than 0.1 mg / L (0.1 ppm) and no I2 or IO3- was eluted. Iodine, the only eluting substance caused by the use of multi-iodine anion resins, is not a compound that accumulates even when fish is absorbed. It is an essential element necessary to make thyroid hormone in humans. In foreign countries, it is added to salt or water, and iodine ions absorbed by fish in small amounts are beneficial to human health. Yod is abundant in seaweeds such as seaweed. Therefore, there is no problem of accumulation and resistance of other antibiotics due to the use of iodide resin.

The useful life of multi-iodine resins was investigated. This test was passed through the tap water and passed through a certain amount of general bacteria 5,000CFU / mL or more, E. coli 50CFU / mL or more was passed through the water to investigate the bactericidal power. After passing through 1,000L of tap water, 10L of microorganism prepared water was passed through and collected and examined for microorganisms. Next, after passing through 5,000L of tap water in a row, 10L of microorganism preparation water was passed through it, followed by microbial irradiation, and repeated experiments up to 20,000L water flow. As a result of the passage of water, the sterilization effect decreased, and after 1,000L water flow, bacterium showed 98.4% and Escherichia coli showed 99.99% sterilization power, but after 20,000L water flow, it showed 78.5% and 82.1%. As the passage of microorganisms increases, the sterilizing power is deteriorated because organic substances or humus in the water are adsorbed by the iodide resin and interfere with contact between the iodide resin and the microorganism.

In the above results, multi-iodine resins are antibiotic and disinfectant substitutes that have no problem of accumulation or resistance after use, and need to remove existing organic substances to increase their lifespan.

The following is the result of the application experiment using the multi-iodine resin.

Example 1: 200 g of a multi-iodine anion resin was immersed in an aquarium 70 cm wide x 150 cm long and 90 cm high, and grown well when 10 goldfish were observed for 1 year. At the beginning of the experiment, the average length was 5.3 ± 1.4cm and one year later, the average length was 6.7 ± 1.9cm.

Therefore, it can be used as a disinfectant and antimicrobial agent in aquaculture farms and can be used by directly immersing it on the floor or by filling it in a suitable package or device.

Example 2 E. coli was not detected in the washing liquid which was injected when 20 g of a multi-iodine anion resin was placed in a hot water tank prepared at 50 CFU / mL of E. coli and used after 2 minutes. The same effect was also obtained when immersed in the water storage tank of the humidifier.

From these results, the polyhydric anion resin can be used as a disinfectant or antibacterial agent for hypertrophic or humidifiers and can be used by directly immersing it in the floor or by filling it in an appropriate package or device.

Example 3: The bacteria of the condensate were put into the condensate tray of the air conditioner and no bacteria were detected.

From these results, it can be seen that the iodine resin can be used as an antiseptic and antimicrobial agent for the condensate receiver of an air conditioner and can be used by directly immersing it in the floor or by filling it in an appropriate package or device. It can also be used as an antiseptic and antibacterial agent for cooling water.

The use of multi-iodine resins for aquaculture has good antiseptic and antimicrobial effects without the formation of any eluents that accumulate or tolerate. In addition, when used in hypertrophy, humidifiers, air conditioners and cooling water, it was very inexpensive and anti-permanent.

Antibiotics used in the existing aquaculture have accumulated and resistant, but the present invention is a harmful, non-accumulable and non-resistant, rather it can contribute greatly to the public health by supplying an appropriate amount of iodine, an essential trace substance.

Claims (6)

Aquaculture (fresh water) using iodine anion resin made by adsorbing I3-, I5- and I7- made by mixing iodine (I2) and iodide ions (I-) in a certain equivalent ratio to a strong anion exchange resin (Dowex, etc.) Use in place of antibiotics in aquaculture and aquaculture). This includes both the application of iodide resin directly to the floor and the use of it in certain devices such as towers and barrels. Use for the disinfectant of various viruses and bacteria in aquaculture (including freshwater and marine farming) using the iodide resin of Claim 1. This includes both the use of iodide resin directly on the floor and the use of it in certain devices such as towers and barrels. Use for disinfectant of hypertrophy using iodide resin of [claim 1]. In this case, the iodide ion resin may be used by filling the pipe from which the bloating solution comes out, or may be connected to the water supply pipe, may be used in a hot water tank, and may include all cases in which these may be used in parallel. Use for the disinfectant of humidifiers using the iodide resin of [claim 1]. At this time, the iodide ion resin can be used to fill the pipe from which the spray solution of the humidifier comes out, can be used in a storage tank, and includes all cases where they can be used in parallel. It is used for disinfectant of Reynerella in cooling water using iodide resin of [claim 1]. In this case, iodide ion resin can be used by filling the circulation pipe of cooling water, can be connected to the water supply pipe, can be used in the cooling water tank, and includes all cases where they can be used in parallel. It is used to disinfect bacteria generated in a large air conditioner by using the iodide resin of [claim 1]. In this case, the iodide resin can be used directly in the condensate receiver of the air conditioner, can be used as a new device, and includes all cases where they can be used in parallel.