RU2563390C1 - Method of preparing bactericide for water disinfection - Google Patents

Abstract

FIELD: chemistry.

SUBSTANCE: method includes treating an ion-exchange resin based on a quaternary ammonium base with an iodine-containing solution. Before treatment, the resin is flooded with saturated NaOH solution. The resin is then washed with distilled water to pH not higher than 8. The resin is then immersed in an aqueous solution of polydimethyl diallyl ammonium ionohydrate, after which the bactericide is washed with distilled water once more.

EFFECT: high effectiveness of effect on microflora.

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Description

The invention relates to the field of water disinfection and can be used to disinfect water in extreme conditions or field conditions, as well as a bactericidal layer when creating filters for complex water treatment.

Traditionally, to solve the problem of disinfection of drinking water, chlorination, heating, ozonation, ultraviolet irradiation, oligodynamic treatment with heavy metal ions or the introduction of certain additives into water are used. However, chlorination or the use of heavy metal ions is extremely harmful to human health, and installations using ultraviolet and ozone are usually expensive and energy-consuming.

A known method of preparing a bactericidal composition comprising a primary coagulating material and a bridging flocculating material, a cationic polymer of a specific molecular weight, a water-soluble alkali, a disinfectant, a silicate, insoluble in water, with a certain ratio of components included in the composition. The most preferred compositions contain, as cationic coagulation agents, for example, chitosan, a chlorine-based bactericidal disinfectant, for example calcium hypochlorite, a water-soluble alkali, a water-insoluble silicate, and also a nutritional supplement or source of nutrients [RF Patent No. 2243830, publ. 03/20/2005].

A known method of creating a composition for the disinfection of drinking water containing sodium or potassium salt of dichloroisocyanuric acid (5-25 wt. Hours), alkali metal chloride (10-75 wt. Hours), additives that enhance the disinfecting effect, alkali metal salt of acetic or phosphoric acid or trichloroisocyanuric acid (1-30 parts by weight), as well as dechlorinating additives (4.5-50 parts by weight), where cysteine and / or ascorbic acid, its alkaline and alkaline earth salts are used as dechlorinating additives metals, as well as mixtures thereof.

The disadvantage of these inventions is their possible toxicity due to the use of chlorine-containing reagents as disinfectants.

Closest to the proposed invention is a method for producing a bactericide by treating an ion-exchange resin based on a quaternary ammonium base with an iodine-containing solution, the solution being prepared by dissolving iodine in 0.1-0.4% alcohol alkali, and the treatment is carried out to obtain a bactericide of the general formula: R ₄ NI _n · ((n-1) / 2) · H ₂ O, where n is an odd integer from 3 to 9, R is an organic radical, N is nitrogen, I is iodine [Patent No. 2213063, publ. 09/27/2003].

This bactericide has a significant bactericidal capacity, however, the iodine landing by the proposed method is possible only on ion-exchange resins with quaternary ammonium bases, limiting its use.

The objective of the present invention is to develop a method for producing a bactericide with an increase in the effectiveness of exposure to microflora and an expanded scope.

This object is achieved in that the anion exchange resin is treated with an iodine-containing polymer.

The method is as follows: anion-exchange resin brand AB-17-8 is poured with a saturated aqueous solution of NaOH. To prepare a saturated solution, 22.8 g of NaOH are dissolved in 100 ml of distilled water, the pH of the solution is> 13. The alkali-treated resin is washed with distilled water, the pH should not exceed 8. Next, the resin is immersed in an aqueous solution of polydimethyldiallylammonium monohydrate with a given concentration of 1: 1 (100 g of resin per 100 ml of polymer solution). For the preparation of a 0.06% polymer solution, 0.3 ml of polydimethyldiallylammonium ion hydrate is dissolved in 100 ml of water, for the preparation of a 0.5% solution, 2.5 ml of polydimethyl diallylammonium ion hydrate is dissolved in 100 ml of water, for a 1% solution, 5 ml of polydimethyl diallylammonium ion hydrate is dissolved in 100 ml of water. The resulting bactericide is washed with distilled water and water is treated. With a slight microbial contamination, it is possible to use a disinfectant composition obtained by treating an anion exchange resin with a 0.06% aqueous solution of polydimethyl diallylammonium ion hydrate, with a significant content of microorganisms, a 0.5-1% aqueous polymer solution is used.

Example 1. Daily culture of E. coli 113-13 at a concentration of 5 * 10 ⁴ μl / ml, prepared according to the turbidity standard of the Tarasovich GISK No. 5, was filtered through a layer of the composition (resin treated with a 0.5% polymer solution) 100 cm ³ . The filtered suspension was sown on MPA in a volume of 0.1 ml by the lawn method. Cultivated for 24 hours at a temperature of 37 ° C. The results were taken into account by the number of colony forming units (CFU). As a result of filtration, the complete destruction of microorganisms in the filtrate was established.

Example 2. Daily culture of E. coli 113-13, prepared in a similar way, but with a concentration of 5 * 10 ² μl / ml, was filtered through a disinfectant layer with a volume of 100 cm ³ , but in this case a resin treated with a 0.06% solution was used polymer. As a result, the complete absence of microorganisms in the filtrate was also established.

The proposed method excludes operations associated with controlling the release of iodine in the washing process of freshly prepared bactericide.

Accounting for the growth of Escherichia coli after treatment with a bactericide Control E. coli CFU / ml (M ± m) Polymer concentration 2% one% 0.5% 0.06% 0.03% 4.8 ± 6.4 × 10 ⁴ 15 ± 0.4 0 0 0 5 ± 0.5 4.95 ± 4.8 × 10 ² 3 ± 0.67 0 0 0 2 ± 0.3

Disinfection of the proposed method is based on the bactericidal activity of polydimethyldiallylammonium ion hydrate, which is a polymer - a polyelectrolyte with a high charge density. In contrast to a polymer chain having a positive charge, ion hydrates have a charge of the opposite sign and, under normal conditions, ensure the electroneutrality of the molecule. When dissolved in water and applied to the surface of any material, the polymer globules unfold and some of the counterions are released. Due to this, the polyelectrolyte is attached by an organic radical to the anion-exchange centers of the resin, and the active centers of the polyelectrolyte containing bioactive iodine are free. Therefore, there is a "doubled" bactericidal effect of the drug. The first arises due to electrostatic forces: since bacteria have a negative membrane charge and the polymer is positive, a strong electrostatic bond is formed between the polymer and the bacterial membrane. The second active factor is bioactive ion hydrates, which themselves are active and effective in controlling microflora. The polymer is not washed off from the granules, holding on tightly to them, since the polydimethyl diallylammonium ion hydrate is landing due to the positive electrostatic charge on the groups of quaternary ammonium bases due to addition via an organic radical. As a result of tests, it was found that the disinfecting and bactericidal effect of polydimethyldiallylammonium ion hydrate in the aquatic environment, as well as its hygienic safety with prolonged use, make it possible to use it effectively in all areas of water treatment.

Claims (1)

A method for producing a bactericide for disinfecting water by treating an ion-exchange resin based on a quaternary ammonium base with an iodine-containing solution, characterized in that prior to the treatment, the resin is filled with saturated NaOH solution obtained by dissolving 22.8 g of NaOH in 100 ml of distilled water, the pH of the solution is above 13 then the resin is washed with distilled water to a pH of not more than 8, the resin is immersed in a 0.06-1% aqueous solution of polydimethyl diallylammonium ion hydrate at a ratio of 1: 1, after which the bactericide is again washed with distilled water oh.