CN101993136B - Bacteria inactivation rate agent, the ablation method of microorganism and the manufacture method of tap water - Google Patents
Bacteria inactivation rate agent, the ablation method of microorganism and the manufacture method of tap water Download PDFInfo
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- CN101993136B CN101993136B CN201010257867.7A CN201010257867A CN101993136B CN 101993136 B CN101993136 B CN 101993136B CN 201010257867 A CN201010257867 A CN 201010257867A CN 101993136 B CN101993136 B CN 101993136B
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- 241000894006 Bacteria Species 0.000 title claims abstract description 86
- 230000002779 inactivation Effects 0.000 title claims abstract description 66
- 244000005700 microbiome Species 0.000 title claims abstract description 63
- 238000000034 method Methods 0.000 title claims abstract description 59
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 48
- 239000008399 tap water Substances 0.000 title claims abstract description 23
- 235000020679 tap water Nutrition 0.000 title claims abstract description 23
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 14
- 238000002679 ablation Methods 0.000 title claims abstract description 10
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- 239000000470 constituent Substances 0.000 claims abstract description 65
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- 241001465754 Metazoa Species 0.000 description 2
- 241001138501 Salmonella enterica Species 0.000 description 2
- XEFQLINVKFYRCS-UHFFFAOYSA-N Triclosan Chemical compound OC1=CC(Cl)=CC=C1OC1=CC=C(Cl)C=C1Cl XEFQLINVKFYRCS-UHFFFAOYSA-N 0.000 description 2
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- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 description 2
- 229910000360 iron(III) sulfate Inorganic materials 0.000 description 2
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- 241000251468 Actinopterygii Species 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241000252203 Clupea harengus Species 0.000 description 1
- 206010010741 Conjunctivitis Diseases 0.000 description 1
- 101710146739 Enterotoxin Proteins 0.000 description 1
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- 229910021578 Iron(III) chloride Inorganic materials 0.000 description 1
- 206010031252 Osteomyelitis Diseases 0.000 description 1
- 206010033078 Otitis media Diseases 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 206010039361 Sacroiliitis Diseases 0.000 description 1
- 241001354013 Salmonella enterica subsp. enterica serovar Enteritidis Species 0.000 description 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 1
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 1
- 206010000269 abscess Diseases 0.000 description 1
- 229910052784 alkaline earth metal Inorganic materials 0.000 description 1
- 150000001342 alkaline earth metals Chemical class 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
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- 210000004556 brain Anatomy 0.000 description 1
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 description 1
- 239000000920 calcium hydroxide Substances 0.000 description 1
- 229910001861 calcium hydroxide Inorganic materials 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
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- 239000013043 chemical agent Substances 0.000 description 1
- VOVNIMMKYYUQIN-UHFFFAOYSA-N chloric acid Chemical compound OCl(=O)=O.OCl(=O)=O VOVNIMMKYYUQIN-UHFFFAOYSA-N 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
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- 239000000147 enterotoxin Substances 0.000 description 1
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- 229940023064 escherichia coli Drugs 0.000 description 1
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- 229940032296 ferric chloride Drugs 0.000 description 1
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- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- VTHJTEIRLNZDEV-UHFFFAOYSA-L magnesium dihydroxide Chemical compound [OH-].[OH-].[Mg+2] VTHJTEIRLNZDEV-UHFFFAOYSA-L 0.000 description 1
- 239000000347 magnesium hydroxide Substances 0.000 description 1
- 229910001862 magnesium hydroxide Inorganic materials 0.000 description 1
- 239000000395 magnesium oxide Substances 0.000 description 1
- CPLXHLVBOLITMK-UHFFFAOYSA-N magnesium oxide Inorganic materials [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 description 1
- AXZKOIWUVFPNLO-UHFFFAOYSA-N magnesium;oxygen(2-) Chemical compound [O-2].[Mg+2] AXZKOIWUVFPNLO-UHFFFAOYSA-N 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 229920000729 poly(L-lysine) polymer Polymers 0.000 description 1
- 229920000447 polyanionic polymer Polymers 0.000 description 1
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- 235000019353 potassium silicate Nutrition 0.000 description 1
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- 150000003242 quaternary ammonium salts Chemical class 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 230000009278 visceral effect Effects 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 229920001351 ε-poly-L-lysine Polymers 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/52—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities
- C02F1/54—Treatment of water, waste water, or sewage by flocculation or precipitation of suspended impurities using organic material
- C02F1/56—Macromolecular compounds
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Agronomy & Crop Science (AREA)
- Hydrology & Water Resources (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Water Treatment By Sorption (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
Abstract
The invention relates to the bacteria inactivation rate agent of the microorganism in a kind of Inactivation in Water easily, the ablation method of microorganism and the manufacture method of tap water.The present invention utilize comprise containing be selected from the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-more than one, the bacteria inactivation rate agent of the constituent of calcium ion donor, sodium ion donor and aluminum ion donor, carry out the microorganism in Inactivation in Water.In addition, the present invention by described bacteria inactivation rate agent containing be selected from fungistat and sterilant more than one, and by the various bacteria inactivation rates in water to potable degree.
Description
Technical field
The present invention relates to a kind of bacteria inactivation rate agent, the ablation method of microorganism and the manufacture method of tap water.
Background technology
In the water of the rivers and creeks of the former water as tap water or domestic water or lakes and marhshes etc., there is the pathogenic bacterias such as staphylococcus (staphylococcus).
Wherein staphylococcus as the various inflammation of humans and animals morbidity bacterium and known to people, such as skin pyesis, conjunctivitis, sinusitis paranasal sinusitis, otitis media, urocystitis, pneumonia, osteomyelitis, sacroiliitis, visceral abscess.Wherein streptococcus aureus (S.aureus) is the important pathogen of the pyesis of humans and animals, and can become the pathogenic bacteria of food poisoning because of its enterotoxin that produce.
According to the tap water quality benchmark specified in water law from the beginning, be suitable for drinking, must staphylococcus in Inactivation in Water, typically use chloric acid (chloricacid) to carry out germicidal treatment.Not good when there is staphylococcus etc. in the domestic waters such as sump water or outdoor bathing place water yet, carry out germicidal treatment so usually same with tap water.
But present situation is, does not popularize, have 1,700,000,000 people cannot guarantee safe tap water in developing country etc. due to water works, it is dead that the food poisoning caused because of tap water causes reaching 5000 people every day, and wherein majority is children.Therefore, expect to develop a kind of easy treatment process to supply stable tap water.In addition, when cannot utilize water works when disasters such as earthquakes, also need a kind of replacement method without the need to power or large-scale equipment.
When carrying out the inactivation treatment of microorganism, usually using microbiotic or sterilization/antiseptic-germicide etc., when chemical agent stronger for inactivating efficacy is used for tap water, having problems in security.
In this condition, need a kind of can easily the microorganism such as deactivation staphylococcus to obtain the method for tap water or domestic water.
The radioactive rays crosslinked of gamma-polyglutamic acid-(γ-polyglutamicacid) and gamma-polyglutamic acid-is the polymkeric substance of anionic property, continually develops out the constituent in this, as main component in recent years.Known described constituent has following functions before this: in sewage wastewater or waterworks water, make dirty substance, metal ion, dioxin (dioxine) class coagulating sedimentation, or condenses in the water containing food or fermentation water and obtain objective food or fermented product (patent documentation 1).
But, still do not know that the cross-linking agent containing gamma-polyglutamic acid-or gamma-polyglutamic acid-can the microorganism such as deactivation staphylococcus as the constituent of main component.[patent documentation 1] Japanese Patent Laid-Open 2002-210307 publication
[patent documentation 2] Japanese Patent Laid-Open 2004-174326 publication
[patent documentation 3] Japanese Patent Laid-Open 2004-202441 publication
As can be seen here, above-mentioned existing bacteria inactivation rate agent and method thereof obviously still have inconvenience and defect, and are urgently further improved.In order to solve above-mentioned Problems existing, relevant manufactures there's no one who doesn't or isn't seeks solution painstakingly, and this is obviously the anxious problem for solving of relevant dealer.Therefore how to found the ablation method of a kind of new bacteria inactivation rate agent and microorganism, one of current important research and development problem of real genus, also becomes the target that current industry pole need be improved.
Summary of the invention
The object of the invention is to, overcome the defect of existing bacteria inactivation rate agent and method existence thereof, and inactivator and the ablation method thereof of the microorganism in a kind of Inactivation in Water easily are newly provided, containing be selected from more than one in the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-can inactivating microbial as the constituent of main component, be very suitable for practicality.
Another object of the present invention is to, a kind of method manufacturing tap water is easily provided.
The object of the invention to solve the technical problems realizes by the following technical solutions.For achieving the above object, according to a kind of bacteria inactivation rate agent of the present invention, comprise containing be selected from the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-more than one, the constituent of calcium ion donor, sodium ion donor and aluminum ion donor.
Aforesaid bacteria inactivation rate agent, also containing be selected from fungistat and sterilant more than one.
Aforesaid bacteria inactivation rate agent, described fungistat and sterilant are cationic compounds.
Aforesaid bacteria inactivation rate agent, described cationic compound is the polypeptide (polypeptide) based on basic aminoacids.
Aforesaid bacteria inactivation rate agent, described polypeptide is epsilon-polylysine (ε-Poly-L-Lysine).
Aforesaid bacteria inactivation rate agent, described constituent and described epsilon-polylysine be 1: 10 ~ 500: 1 containing mass ratio.
The object of the invention to solve the technical problems also adopts following technical scheme to realize.For achieving the above object, according to the ablation method of a kind of microorganism of the present invention, it is microorganism described in deactivation in containing the water of microorganism, comprise make containing be selected from the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-more than one, the step of the water of constituent contact containing described microorganism of calcium ion donor, sodium ion donor and aluminum ion donor.
Aforesaid method, the step that described constituent is contacted comprises: in the water containing described microorganism, add the step of described constituent and stir the step of the water after described interpolation.
Aforesaid method, described microorganism is staphylococcus.
Aforesaid method, also comprises: in the water containing described microorganism, add more than one the step be selected from fungistat and sterilant.
Aforesaid method, by containing be selected from the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-more than one, the constituent of calcium ion donor, sodium ion donor and aluminum ion donor and be selected from fungistat and sterilant more than one add in water simultaneously.
Aforesaid method, described fungistat and sterilant are cationic compounds.
Aforesaid method, is characterized in that: described cationic compound is the polypeptide based on basic aminoacids.
Aforesaid method, described polypeptide is epsilon-polylysine.
Aforesaid method, to make the concentration of described constituent for 1ppm ~ 50000ppm, the concentration of described epsilon-polylysine is that the mode of 0.1ppm ~ 100ppm is added.
Aforesaid method, with make the mass concentration ratio of described constituent and described epsilon-polylysine be 1: 10 ~ 500: 1 mode add.
The object of the invention to solve the technical problems also realizes in addition by the following technical solutions.For achieving the above object, the manufacture method of a kind of tap water proposed according to the present invention, uses above-mentioned method to manufacture tap water.
The present invention compared with prior art has obvious advantage and beneficial effect.By technique scheme, the present invention at least has following advantages and beneficial effect:
According to the present invention, the method for microorganism in a kind of Inactivation in Water easily can be provided, thus tap water or domestic water can be obtained easily.
In sum, the invention provides the method for microorganism in a kind of Inactivation in Water easily.In addition, the present invention also provides a kind of method manufacturing tap water easily.The present invention utilize comprise containing be selected from the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-more than one, the bacteria inactivation rate agent of the constituent of calcium ion donor, sodium ion donor and aluminum ion donor, carry out the microorganism in Inactivation in Water.
Above-mentioned explanation is only the general introduction of technical solution of the present invention, in order to technique means of the present invention can be better understood, and can be implemented according to the content of specification sheets, and can become apparent to allow above and other object of the present invention, feature and advantage, below especially exemplified by preferred embodiment, and coordinate accompanying drawing, be described in detail as follows.
Accompanying drawing explanation
Nothing
Embodiment
For further setting forth the present invention for the technique means reaching predetermined goal of the invention and take and effect, below in conjunction with accompanying drawing and preferred embodiment, to the bacteria inactivation rate agent proposed according to the present invention, the ablation method of microorganism and its embodiment of the manufacture method of tap water, composition, method, step, feature and effect thereof, be described in detail as follows.
Bacteria inactivation rate agent of the present invention comprise containing be selected from more than one in the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-as the constituent of main component.Specifically, comprise containing be selected from the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-more than one, the constituent of calcium ion donor, sodium ion donor and aluminum ion donor.In addition, in the cross-linking agent of described gamma-polyglutamic acid-and described gamma-polyglutamic acid-, part or all of gamma-polyglutamic acid-can be salt.
Gamma-polyglutamic acid-in the constituent of bacteria inactivation rate agent of the present invention, such as can by manufacturing as the known methods such as the fermentation process of microorganism or chemical synthesis that utilize disclosed in Japanese Patent Laid-Open 2002-210307 publication.
The weight-average molecular weight of gamma-polyglutamic acid-preferably utilize gel permeation chromatography (gelpermeationchromatography) to be determined as to be distributed in hundreds thousand of ~ millions of between, and be preferably less than 1,000 ten thousand.Also the amino acid beyond L-glutamic acid can be comprised in gamma-polyglutamic acid-.Amino acid now beyond L-glutamic acid is preferably below 10 quality %.
The cross-linking agent of gamma-polyglutamic acid-or the cross-linking agent of gamma-polyglutamic acid-salt such as can as in Japanese Patent Laid-Open 2002-210307 publication disclose as, utilize radioactive rays to make gamma-polyglutamic acid-salt be cross-linked to manufacture.As Japanese Patent Laid-Open 2002-210307 publication disclose, the described gamma-polyglutamic acid-salt that is cross-linked can be obtained by the neutralization reaction of gamma-polyglutamic acid-and basic cpd.Described basic cpd can enumerate the oxyhydroxide of basic metal or alkaline-earth metal, and concrete example can be enumerated: the basic cpd etc. of the Organics such as sodium hydroxide, potassium hydroxide, magnesium hydroxide, calcium hydroxide, hydrated barta etc. or amine.
The straight chain of the preferred gamma-polyglutamic acid-salt compound of cross-linking agent of gamma-polyglutamic acid-is cross-linked the degree of crosslinking of link 2 ~ about 1000.The weight-average molecular weight of the cross-linking agent of gamma-polyglutamic acid-preferably utilizes gel permeation chromatography to be less than 1,000 ten thousand ~ 10,000 ten thousand.
In the constituent of bacteria inactivation rate agent of the present invention, be selected from more than one the content preferably 0.1 quality % ~ 30 quality % in the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-, more preferably 0.5 quality % ~ 20 quality %.
Calcium ion donor in the constituent of bacteria inactivation rate agent of the present invention can be enumerated: calcium sulfate, calcium carbonate, calcium phosphate etc., can use one or more in these.Preferred use calcium sulfate and calcium carbonate.In the constituent of bacteria inactivation rate agent of the present invention, the content of calcium ion donor is preferably 40 quality % ~ 90 quality %, more preferably 80 quality % ~ 90 quality %.
Sodium ion donor in the constituent of bacteria inactivation rate agent of the present invention can be enumerated: sodium carbonate, sodium bicarbonate, sodium-chlor, water glass, sodium phosphate etc., can use one or more in these.Preferred use sodium carbonate.In the constituent of bacteria inactivation rate agent of the present invention, the content of sodium ion donor is preferably 0.1 quality % ~ 20 quality %, more preferably 0.1 quality % ~ 10 quality %.
Aluminum ion donor in the constituent of bacteria inactivation rate agent of the present invention can be enumerated: Tai-Ace S 150, aluminum chloride, poly aluminium chloride etc., can use wherein one or more.Preferred use Tai-Ace S 150.In the constituent of bacteria inactivation rate agent of the present invention, the content of aluminum ion donor is preferably 0.01 quality % ~ 10 quality %, more preferably 0.1 quality % ~ 5 quality %.
In addition, the constituent of bacteria inactivation rate agent of the present invention, except above-described metal ion donor, can also contain iron ion donor and/or magnesium ion donor.Iron ion donor can enumerate ferric sulfate (ferricsulfate), iron(ic) chloride (ferricchloride) etc., and magnesium ion donor can enumerate magnesium oxide, magnesium chloride etc.
The preferred component of the constituent of bacteria inactivation rate agent of the present invention and the content of preferred component as follows: the cross-linking agent of gamma-polyglutamic acid-is 1 quality % ~ 10 quality %, calcium sulfate is 70 quality % ~ 80 quality %, calcium carbonate is 10 quality % ~ 20 quality %, sodium carbonate is 1 quality % ~ 10 quality %, Tai-Ace S 150 is 0.1 quality % ~ 3 quality %.This constituent can obtain commercially available product PG α 21Ca (Japanese Bao Ligu limited-liability company (Poly-GluCo., Ltd)).
Bacteria inactivation rate agent of the present invention forms suspended particles in water, and the microbes in water is adsorbed on these suspended particles.Be adsorbed on microorganism on described suspended particles to precipitate by leaving standstill, so in the supernatant liquor of water after treatment, living microorganism disappears.In this specification sheets, so-called inactivating microbial, not only refers to carry out antibacterial and/or sterilization to microorganism, also comprises the state that there is not living microorganism (being removed) in the supernatant liquor of the water reach process as the above after.Bacteria inactivation rate agent of the present invention particularly removes and the excellent effect of deactivation staphylococcus or yeast.This effect be containing be selected from more than one in the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-as the function of constituent dawn unknown by the people before this of main component.
Bacteria inactivation rate agent of the present invention can also containing be selected from fungistat and sterilant more than one.As described fungistat and sterilant, the fungistat of the microorganism beyond preferred deactivation staphylococcus and sterilant.By using containing be selected from more than one in the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-as main component constituent and be selected from fungistat and sterilant more than one combined, can deactivation multiple-microorganism.
Wherein, fungistat and the sterilant material that uses the Human Physiologies such as food additives to allow.The fungistat used in the present invention and sterilant such as can be enumerated: cationic compound, grapefruit seed extract, triclosan (triclosan) etc.In addition, cationic compound can enumerate polycationic compound or quaternary ammonium salt etc., polycationic compound can enumerate the polypeptide based on basic aminoacids, and preferred polypeptide can illustrate the epsilon-polylysine, protamine (protamine) etc. that its more than 60% mole of forming is basic aminoacids.
Known epsilon-polylysine has germicidal action, is therefore used as food additives (Japanese Patent Laid-Open No. Sho 63-109762 publication).
In addition, protamine is the general name of the histone sample peptide of the sperm nucleus coming from the fish such as salmon (salmon) or catfish (herring), is the polypeptide with rich arginine structure.Known protamine also has germ resistance, is also used as food additives (T.Motohiro:BioIndustry, 6 (2), 5 (1989)).
The polymerization degree of these polypeptide is not particularly limited, and preferably 1000 ~ 5000.
As shown in following example (test example 2), these materials deactivation can be present in the microorganism such as intestinal bacteria (Bacilluscoli) or Salmonellas (Salmonella) in water.Therefore, by in bacteria inactivation rate agent of the present invention, using these materials with containing be selected from more than one in the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-combinationally use as the constituent of main component, deactivation easily can be present in various microorganisms in water.
Particularly epsilon-polylysine is different with common chlorine system sterilant, and its inactivating efficacy can maintain the long period, therefore preferably uses.In other words, need not add to add and just can preserve the water after inactivation treatment for a long time, and the microbial contamination that occurs after treatment can be suppressed, therefore be suitable for the purposes of processing drinking water or domestic water.
As shown in following example (test example 3), although epsilon-polylysine and protamine are polycationic, even but coexist with the gamma-polyglutamic acid-of polyanion, the cross-linking agent etc. of gamma-polyglutamic acid-, also function each other of can not cancelling out each other and playing a role, this point is the phenomenon exceeding the anticipation set about when carrying out correlative study of the present invention, is that the present inventors want ben discovery.
The microorganism deactivation together that the bacteria inactivation rate agent of the present invention of more than one the form containing being selected from fungistat and sterilant described above can may will exist in waterworks water, sewage wastewater, rivers and creeks, lakes and marhshes etc. usually.Specifically, the bacteria inactivation rate agent of the present invention of described form can deactivation: the Gram-negative bacterias (Gram-negativeorganism) such as intestinal bacteria, Salmonellas, Pseudomonas aeruginosa (Pseudomonasaeruginosa), Klebsiella Pneumoniae (Klebsiellapneumoniae); The gram-positive microorganism (Gram-positiveorganism) such as staphylococcus, subtilis (Bacillussubtilis), wax printing fabric (Bacilluscereus), listeria bacteria (Listeriamonocytogenes); And the microorganism such as yeast.
Bacteria inactivation rate agent of the present invention can adopt can using containing be selected from more than one in the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-as main component constituent and be selected from more than one in fungistat and sterilant forms of group cover (kit) of simultaneously using, also can adopt the form be contained in a blender.Blender form can by described constituent be selected from fungistat and sterilant together with more than one to use, comparatively easy, therefore preferably.
The ablation method of microorganism of the present invention comprises: make containing be selected from the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-more than one, the step of the water of the constituent of calcium ion donor, sodium ion donor and aluminum ion donor contact containing microorganism.The step that described constituent is contacted can by carrying out to containing the step of adding the step of described constituent and the water after stirring interpolation in the water of microorganism, also can by the water containing microorganism is stored in secure described constituent aqua storage tank (container) in carry out, the water containing microorganism can also be made to be undertaken by the passage (water injection pipe, water shoot or water circulation path etc.) securing described constituent.Consider from the simplicity of operation, carry out preferably by containing the step of adding the step of described constituent and the water after stirring interpolation in the water of microorganism.After the step that this makes described constituent contact, the step of standing water can also be carried out.As shown in following example (test example 1), utilize present method can microorganism in Inactivation in Water, particularly can deactivation staphylococcus or yeast.
In addition, containing be selected from the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-more than one, the constituent of calcium ion donor, sodium ion donor and aluminum ion donor and be selected from fungistat and sterilant more than one can add simultaneously or add successively.Owing to adding the inactivation treatment can carrying out microorganism easily simultaneously, therefore preferably.
By containing be selected from the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-more than one, in the method that simultaneously uses of the constituent of calcium ion donor, sodium ion donor and aluminum ion donor and poly-L-Lysine, by adopting following form can microorganism for a long time in Inactivation in Water.Such as can consider the form be positioned over by the epsilon-polylysine be fixed on fiber etc. in aqua storage tank (container).Described aqua storage tank can be enumerated: supply tank, pond, sprinkling basin, pond etc.In addition, it is also conceivable to following form: made epsilon-polylysine be present in the water circulation paths such as the off-premises station of various water injection pipe, water shoot or air conditioning machinery (airconditioning) by fixing grade, make water containing microorganism by these streams, thus the microorganism also deactivation that will newly contact.
The method of the application of the invention, can obtain the microorganism tap water of deactivation or domestic water easily.Now, the water after using method of the present invention to process can under the state being added with inactivator, supernatant liquor is supplied to drink or pond, outdoor bathing place purposes.Certainly, after also can filtering further after treatment, filtrate supply is drunk.
When using method of the present invention to manufacture tap water, former water not only can use the Natural Water such as rivers and creeks or lakes and marhshes, also comprises waterworks water or sewage wastewater.
In the method for the invention, containing be selected from more than one in the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-as the constituent of main component, can be contained in water with any concentration in the scope of bacteria inactivation rate agent allowed by human body.Particularly when deactivation staphylococcus, that to make to be selected from more than one relative concentration in the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-in the total amount of water constituent be therewith 1ppm ~ 50000ppm, preferred 10ppm ~ 10000ppm, particularly preferably the mode of 50ppm ~ 5000ppm is added.Within the scope of this, such as, for comprising staphylococcus 10
6the water of individual/more than mL, can by this staphylococcus deactivation to drinking allowed 10
3individual/below mL.To add after described constituent after 30 minutes, preferably can obtain described effect after 2 hours.
In the method for the invention, more than one being selected from fungistat and sterilant can be contained in water with any concentration in the scope allowed by human body.Particularly when bacteria inactivation rate agent is epsilon-polylysine, be with the relative concentration of epsilon-polylysine in the total amount of water, this constituent and epsilon-polylysine for 0.1ppm ~ 100ppm, the mode of preferred 0.5ppm ~ 50ppm is added.Within the scope of this, such as, for comprising intestinal bacteria and Salmonellas each 10
7the water of individual/more than mL, can by these bacterium deactivations to drinking allowed 10
2individual/below mL.Add described constituent after 1 hour, preferably can obtain described effect after 2 hours.
In addition, in the method for the invention, when interpolation is containing when being selected from more than one in the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-constituents as main component and the epsilon-polylysine as sterilant, be make the relative concentration of described constituent in the total amount of water constituent be therewith 1ppm ~ 50000ppm, preferred 10ppm ~ 10000ppm, particularly preferably 50ppm ~ 5000ppm, make the relative concentration of described epsilon-polylysine in water, the total amount of this constituent and epsilon-polylysine is 0.1ppm ~ 100ppm, the mode of preferred 0.5ppm ~ 50ppm is added.Further, be make the mass concentration ratio of described constituent and epsilon-polylysine 1: 10 ~ 500: 1, preferably 1: 2 ~ 200: 1, more preferably 2.5: 1 ~ 200: 1 scope in mode add.Within the scope of this, such as, for comprising staphylococcus, intestinal bacteria, Salmonellas each 10
6the water of individual/more than mL, can by these bacterium deactivations to drinking allowed 10
2individual/below mL.To add after described constituent after 1 hour, preferably can obtain described effect after 2 hours.
And method of the present invention all can be suitable for for the water of any pH value, being particularly suitable for using pH value 6 ~ 9 times, is preferably use for 6.5 ~ 8 times in pH value.
In addition, method of the present invention all can be suitable for for the water of arbitrary temp, as long as under room temperature, normal temperature, even if not heat especially, cool and also can process fully.
Following illustrative example illustrates in greater detail the present invention, but the present invention is not limited to these examples.
In following each test, the inactivating efficacy of microorganism measures according to following operation.
< tests the preparation > of microorganism
Each test organisms is cultivated in plain broth substratum (bouillonmedia), under the optimal temperature of each test organisms, carries out shaking culture till the logarithmic proliferation later stage.From then on collect thalline in bacterium liquid, after cleaning 2 times ~ 3 times with physiological saline 1.5mL (centrifugation 13000rpm, 1 minute), thalline is suspended in physiological saline 1.5mL.From described suspension, take out 1mL, be added in sterilized water 9mL, fully stir (diluent making 10-1).The diluent of 10-2 ~ 10-8 is prepared successively by the diluent of described 10-1.Each diluent is smeared respectively 1mL to Membrance cuiture base (SANITAKUN, intestinal bacteria/coliform is used, streptococcus aureus is used, Salmonellas is used, common bacterium is used or fungi is used: Chisso Corp (CHISSOCORPORATION)) on, cultivate under the optimal temperature of each test organisms after 24 ~ 72 hours, measure the bacterium number on Membrance cuiture base.
The mensuration > of < bacteria inactivation rate effect
Sterilized water 500mL is added, inoculation 10 in sterile beaker
6individual/mL ~ 10
10the bacterium liquid 0.20mL of individual/mL, is adjusted to 10 by initial bacterium number
3individual/mL ~ 10
7individual/mL.In addition, when without special instruction, pH value is 6 ~ 8.After slow stirring 5 minutes, add PG α 21Ca (Japanese Bao Ligu limited-liability company) and/or fungistat or sterilant to reach the final concentration of regulation.After at room temperature slowly stirring 10 minutes ~ 15 minutes, leave standstill specific time.Extract supernatant liquor, with antiseptic-germicide deactivation substratum (brain heart infusion (BrianHeartInfusion, BHI) substratum) etc.) suitably after dilution, on Membrance cuiture base, smear 1mL respectively.Cultivate under the optimal temperature of each test organisms after 24 hours ~ 72 hours, measure the bacterium number (individual/mL) on Membrance cuiture base.
In addition, PG α 21Ca's is composed as follows, is namely the mixture that the cross-linking agent of gamma-polyglutamic acid-is 1 quality % ~ 10 quality %, calcium sulfate is 70 quality % ~ 80 quality %, calcium carbonate is 10 quality % ~ 20 quality %, sodium carbonate is 1 quality % ~ 10 quality %, Tai-Ace S 150 is less than 3 quality %.
In addition, fungistat or sterilant use following material.
Epsilon-polylysine: the epsilon-polylysine powder of 50%, the epsilon-polylysine aqueous solution (Chisso Corp) of 25%
Protamine: the protamine sulfate (Babcock and Wilcox: with Guang Chun medicine Industries, Inc) coming from salmon
Grapefruit seed extract: DF-100 (Babcock and Wilcox: ChemieResearch & ManufacturingCoInc)
In addition, test organisms uses intestinal bacteria (Escherichiacoli), Salmonellas (Salmonella enteritidis (Salmonellaenterica), Salmonella choleraesuls (Salmonellacholeraesuis)), Pseudomonas aeruginosa (Pseudomonasaeruginosa), Klebsiella Pneumoniae (Klebsiellapneumoniae), staphylococcus (streptococcus aureus (Staphylococcusaureus)), subtilis (Bacillussubtilis), wax printing fabric (Bacilluscereus), listeria bacteria (Listeriamonocytogenes), yeast (Candida albicans (Candidaalbicans), Pichia anomala (Pichiaanomala)).
The effect when constituent of bacteria inactivation rate agent of the present invention is used alone by < test example 1>
(1) to comprising in intestinal bacteria, Salmonellas, staphylococcus, Pseudomonas aeruginosa, wax printing fabric, Klebsiella Pneumoniae or listerial water, independent interpolation, as the PG α 21Ca of the constituent of bacteria inactivation rate agent of the present invention, studies inactivating efficacy now.Result is shown in table 1.Find that these microorganisms are by PG α 21Ca deactivation, particularly obtain higher effect for staphylococcus or Pseudomonas aeruginosa.
Table 1
(2) in the water comprising yeast, the PG α 21Ca as the constituent of bacteria inactivation rate agent of the present invention is also added separately, research inactivating efficacy now.Result is shown in table 2.Find thus, bacteria inactivation rate agent of the present invention also has inactivating efficacy slowly for yeast.
Table 2
Effect when fungistat or sterilant are used alone by < test example 2>
(1) bacteria inactivation rate effect when fungistat or sterilant (epsilon-polylysine, protamine, the grapefruit seed extract) of bacteria inactivation rate agent of the present invention is added separately in research in the water (pH value is 8) comprising various microorganism.Each interpolation concentration of fungistat or sterilant is 10ppm.Result is shown in table 3.Find thus, described fungistat or sterilant can by with 10 with the concentration of 10ppm
4individual/mL ~ 10
5individual/mL is contained in the abundant deactivation of microorganism in water.
In addition, in other pH value range, be pH value 6 ~ 9 at epsilon-polylysine, protamine is pH value 6 ~ 9, and grapefruit seed extract is that pH value shows same bacteria inactivation rate effect for 5 ~ 9 times.
Table 3
The initial bacterium number of ※: * epsilon-polylysine and protamine; * grapefruit seed extract
(2) in the water comprising various microorganism, the epsilon-polylysine as the sterilant of bacteria inactivation rate agent of the present invention is added separately, research inactivating efficacy now.Result is shown in table 4 and table 5.Find thus, epsilon-polylysine promptly can show inactivating efficacy for the microorganism usually existed in rivers and creeks or lakes and marhshes, but then effect is slow for staphylococcus.
Table 4
Table 5
(3) to comprising in intestinal bacteria, Salmonellas or staphylococcic water, independent interpolation is as the epsilon-polylysine of the sterilant of bacteria inactivation rate agent of the present invention, described each microorganism is being added further, research inactivating efficacy now after the specified time.Additional microorganism carries out after being each sampling after 24 hours, 48 hours, 72 hours and 144 hours.In addition, the interpolation concentration of epsilon-polylysine is 5ppm.Result is shown in table 6.
Find thus, the deactivation of epsilon-polylysine can continue for a long time, even and if do not add epsilon-polylysine, also effect is shown to the new bacterium added.
Also the effect when bacteria inactivation rate agent containing sterilant uses by < test example 3> simultaneously
(1) to comprising intestinal bacteria, Salmonellas or staphylococcus (each initial bacterium number 10
3~ 10
6level/mL) water in, add PG α 21Ca50ppm or 100ppm and epsilon-polylysine 0ppm ~ 5ppm, research inactivating efficacy now.In described scope, bacterium number of surviving after 2 hours at the latest roughly in all cases becomes and is less than 10/mL.As representative examples, to comprising intestinal bacteria, (initial bacterium number is 1.1 × 10
6individual/mL), (initial bacterium number is 5.2 × 10 to Salmonellas
6individual/mL) or staphylococcus (initial bacterium number is 2.6 × 10
6individual/mL) water in, add PG α 21Ca50ppm and epsilon-polylysine 0ppm ~ 5ppm, show the result in table 7.In addition, to comprising intestinal bacteria, (initial bacterium number is 7.6 × 10
6individual/mL), (initial bacterium number is 1.5 × 10 to Salmonellas
6individual/mL) or staphylococcus (initial bacterium number is 1.4 × 10
6individual/mL) water in add PG α 21Ca100ppm and epsilon-polylysine 0ppm ~ 5ppm, show the result in table 8.
Find thus, being in the scope of 1: 10 ~ 500: 1 containing more than a kind that is selected from the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-constituent as main component and the mass concentration ratio of the epsilon-polylysine as sterilant, can by the bacteria inactivation rate in water to the degree that can be used for drinking.
Table 7
Table 8
(2) in the water comprising yeast, PG α 21Ca50ppm or 100ppm and epsilon-polylysine 0ppm ~ 100ppm is added, research inactivating efficacy is now shown in table 9 for the result of Candida albicans (C.albicans), and the result for Pichia anomala (P.anomala) is shown in table 10.
Find thus, being in the scope of 1: 10 ~ 500: 1 containing more than a kind that is selected from the cross-linking agent of gamma-polyglutamic acid-and gamma-polyglutamic acid-constituent as main component and the mass concentration ratio of the epsilon-polylysine as sterilant, can by the deactivation of the yeast in water to the degree that can be used for drinking.
Table 9
Table 10
(3) to comprising in intestinal bacteria, Salmonellas or staphylococcic water, adding PG α 21Ca50ppm or 100ppm and epsilon-polylysine 5ppm, after the specified time, adding described each microorganism further, research inactivating efficacy now.Additional microorganism carries out after being each sampling after 24 hours, 48 hours and 72 hours.The result that the PG α 21Ca concentration added is 50ppm and epsilon-polylysine when being 5ppm is shown in table 11, and result when adding PG α 21Ca100ppm and epsilon-polylysine 5ppm is shown in table 12.Identical with the described situation of epsilon-polylysine that only uses, the effect of bacteria inactivation rate agent of the present invention can continue for a long time, even if do not add inactivator, also shows effect to the new bacterium added.
[utilizability in industry]
According to the present invention, can microorganism easily in Inactivation in Water, also can obtain tap water or domestic water easily, therefore industrially very useful.
The above, it is only preferred embodiments of the present invention, not any pro forma restriction is done to the present invention, although the present invention discloses as above with preferred embodiments, but and be not used to limit the present invention, any those skilled in the art, do not departing within the scope of technical solution of the present invention, when the structure and technology contents that can utilize described announcement are made a little change or be modified to the equivalent example of equivalent variations, but every content not departing from technical solution of the present invention, according to any simple modification that technical spirit of the present invention is done above example, equivalent variations and modification, all still belong in the scope of technical solution of the present invention.
Claims (7)
1. a bacteria inactivation rate agent, it is characterized in that: the cross-linking agent comprising gamma-polyglutamic acid-is 1 quality % ~ 10 quality %, calcium sulfate is 70 quality % ~ 80 quality %, calcium carbonate is 10 quality % ~ 20 quality %, sodium carbonate is 1 quality % ~ 10 quality %, Tai-Ace S 150 is less than 3 quality % constituent and epsilon-polylysine, wherein said constituent and described epsilon-polylysine be 1: 2 ~ 200: 1 containing mass ratio, and described bacteria inactivation rate agent is applicable to inactivating microbial.
2. the ablation method of a microorganism, it is characterized in that: it is microorganism described in deactivation in containing the water of microorganism, comprise the step making the cross-linking agent of gamma-polyglutamic acid-is 1 quality % ~ 10 quality %, calcium sulfate is 70 quality % ~ 80 quality %, calcium carbonate is 10 quality % ~ 20 quality %, sodium carbonate is 1 quality % ~ 10 quality %, Tai-Ace S 150 is less than 3 quality % constituent and the water of epsilon-polylysine contact containing described microorganism, the mode being wherein 1: 2 ~ 200: 1 with the mass concentration ratio of described constituent and described epsilon-polylysine is added.
3. method according to claim 2, is characterized in that the step that described constituent is contacted comprises: in the water containing described microorganism, add the step of described constituent and stir the step of the water after described interpolation.
4. according to the method in claim 2 or 3, it is characterized in that: described microorganism is staphylococcus.
5. method according to claim 2, is characterized in that: be 1 quality % ~ 10 quality % by the cross-linking agent of gamma-polyglutamic acid-, calcium sulfate is 70 quality % ~ 80 quality %, calcium carbonate is 10 quality % ~ 20 quality %, sodium carbonate is 1 quality % ~ 10 quality %, constituent that Tai-Ace S 150 is less than 3 quality % adds in water with epsilon-polylysine simultaneously and contact water.
6. method according to claim 2, is characterized in that: with the concentration of described constituent for 1ppm ~ 50000ppm, and the concentration of described epsilon-polylysine is that the mode of 0.1ppm ~ 100ppm is added.
7. a manufacture method for tap water, is characterized in that: use the method according to any one of claim 2 to 6 to manufacture tap water.
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| TWI568809B (en) * | 2013-02-12 | 2017-02-01 | 東洋紡股份有限公司 | Virus inactivating agent |
| TWI725542B (en) * | 2018-09-25 | 2021-04-21 | 日商東洋紡股份有限公司 | Water-dispersible particles, antimicrobial agents and biofilm removers |
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| JP3596147B2 (en) * | 1996-02-15 | 2004-12-02 | チッソ株式会社 | Antibacterial filter |
| JP3854466B2 (en) * | 2001-01-22 | 2006-12-06 | 小田 節子 | Flocculant and flocculation method |
| JP3836072B2 (en) * | 2002-11-25 | 2006-10-18 | 日本ポリグル株式会社 | Water purification method |
| JP4381154B2 (en) * | 2004-01-21 | 2009-12-09 | 日本ポリグル株式会社 | Method for recovering aggregates in water and recovery tool for aggregates in water used therefor |
| JP4490795B2 (en) * | 2004-11-18 | 2010-06-30 | 日本ポリグル株式会社 | Water purification method |
| JP4965129B2 (en) * | 2006-01-24 | 2012-07-04 | 広人 前田 | Antifouling agent |
| JP2007297559A (en) * | 2006-05-02 | 2007-11-15 | Rohto Pharmaceut Co Ltd | AMINO ACID-MODIFIED-(gamma-POLYGLUTAMIC ACID) OR ITS SALT AND THEIR USES |
| JP2008093625A (en) * | 2006-10-16 | 2008-04-24 | Nippon Poly-Glu Co Ltd | Method for cleaning waste water containing coloring matter component |
-
2009
- 2009-08-20 JP JP2009190719A patent/JP2011042603A/en active Pending
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2010
- 2010-08-18 US US12/859,128 patent/US20110046040A1/en not_active Abandoned
- 2010-08-18 CN CN201010257867.7A patent/CN101993136B/en not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
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| Water Disinfection for International and Wilderness Travelers;Howard Backer;《travel medicine》;20020201;第34卷;第355页-第364页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101993136A (en) | 2011-03-30 |
| US20110046040A1 (en) | 2011-02-24 |
| JP2011042603A (en) | 2011-03-03 |
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