KR102840377B1 - 개선된 특성을 갖는 오메가-하이드록실라제-관련 융합 폴리펩티드 변이체 - Google Patents

개선된 특성을 갖는 오메가-하이드록실라제-관련 융합 폴리펩티드 변이체

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KR102840377B1
KR102840377B1 KR1020187020089A KR20187020089A KR102840377B1 KR 102840377 B1 KR102840377 B1 KR 102840377B1 KR 1020187020089 A KR1020187020089 A KR 1020187020089A KR 20187020089 A KR20187020089 A KR 20187020089A KR 102840377 B1 KR102840377 B1 KR 102840377B1
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seq
fatty acid
cyp153a
fusion polypeptide
hybrid fusion
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KR20180132604A (ko
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안드레스 쉬르머
바오롱 주
에마뉴엘라 포포바
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게노마티카 인코포레이티드
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    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/62—DNA sequences coding for fusion proteins
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
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    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
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    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01005—Lysophospholipase (3.1.1.5)
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    • C12Y301/02—Thioester hydrolases (3.1.2)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C12Y301/02014—Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
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    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)

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  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
KR1020187020089A 2015-12-15 2016-12-13 개선된 특성을 갖는 오메가-하이드록실라제-관련 융합 폴리펩티드 변이체 Active KR102840377B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020257025119A KR20250117480A (ko) 2015-12-15 2016-12-13 개선된 특성을 갖는 오메가-하이드록실라제-관련 융합 폴리펩티드 변이체

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
PCT/EP2015/079832 WO2017101987A1 (en) 2015-12-15 2015-12-15 Omega-hydroxylase-related fusion polypeptide variants with improved properties
EPPCT/EP2015/079832 2015-12-15
PCT/US2016/066405 WO2017106205A1 (en) 2015-12-15 2016-12-13 Omega-hydroxylase-related fusion polypeptide variants with improved properties

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KR20180132604A KR20180132604A (ko) 2018-12-12
KR102840377B1 true KR102840377B1 (ko) 2025-08-01

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KR1020257025119A Pending KR20250117480A (ko) 2015-12-15 2016-12-13 개선된 특성을 갖는 오메가-하이드록실라제-관련 융합 폴리펩티드 변이체

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US (3) US10787648B2 (https=)
EP (2) EP4026899A1 (https=)
JP (2) JP6957469B2 (https=)
KR (2) KR102840377B1 (https=)
CN (3) CN108699536B (https=)
AU (1) AU2016370490B2 (https=)
CA (1) CA3008836A1 (https=)
ES (1) ES2908400T3 (https=)
MX (1) MX2018007298A (https=)
MY (2) MY196584A (https=)
SI (1) SI3390620T1 (https=)
WO (2) WO2017101987A1 (https=)

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SI2984490T1 (sl) 2013-06-14 2020-10-30 Genomatica, Inc. Metode proizvodnje derivatov omega hidroksilirane maščobne kisline
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WO2017101987A1 (en) * 2015-12-15 2017-06-22 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptide variants with improved properties
US20210189439A1 (en) * 2018-05-10 2021-06-24 Genomatica, Inc. Multifunctional Fatty Acid Derivatives And Biosynthesis Thereof
KR102516485B1 (ko) 2018-10-31 2023-04-03 삼성전자주식회사 폴더블 하우징을 포함하는 전자 장치

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