KR102785418B1 - 표적 단백질을 안정적으로 발현할 수 있는 재조합 바이러스 - Google Patents

표적 단백질을 안정적으로 발현할 수 있는 재조합 바이러스 Download PDF

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KR102785418B1
KR102785418B1 KR1020207029714A KR20207029714A KR102785418B1 KR 102785418 B1 KR102785418 B1 KR 102785418B1 KR 1020207029714 A KR1020207029714 A KR 1020207029714A KR 20207029714 A KR20207029714 A KR 20207029714A KR 102785418 B1 KR102785418 B1 KR 102785418B1
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gene
polynucleotide
recombinant virus
ehv
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KR20200135412A (ko
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광강 마
썬 유안
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베링거 잉겔하임 베트메디카 (차이나) 코포레이션 리미티드
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    • C12N2770/32011—Picornaviridae
    • C12N2770/32111—Aphthovirus, e.g. footandmouth disease virus
    • C12N2770/32134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein

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KR1020207029714A 2018-03-19 2019-03-14 표적 단백질을 안정적으로 발현할 수 있는 재조합 바이러스 Active KR102785418B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN2018079410 2018-03-19
CNPCT/CN2018/079410 2018-03-19
PCT/CN2019/078057 WO2019179345A1 (en) 2018-03-19 2019-03-14 Recombinant virus capable of stably expressing target proteins

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KR20200135412A KR20200135412A (ko) 2020-12-02
KR102785418B1 true KR102785418B1 (ko) 2025-03-26

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US (1) US11904010B2 (https=)
EP (1) EP3768831A4 (https=)
JP (1) JP7387623B2 (https=)
KR (1) KR102785418B1 (https=)
CN (1) CN111902535B (https=)
AU (1) AU2019237413C1 (https=)
CA (1) CA3094416A1 (https=)
MX (1) MX2020009720A (https=)
WO (1) WO2019179345A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3094416A1 (en) * 2018-03-19 2019-09-26 Boehringer Ingelheim Vetmedica (China) Co., Ltd. Recombinant virus capable of stably expressing target proteins
CA3162622A1 (en) * 2019-11-25 2021-06-03 Baylor College Of Medicine Selective expansion of gene-targeted cells
CN112430625B (zh) * 2020-11-23 2022-08-05 武汉枢密脑科学技术有限公司 含有变异猪伪狂犬病病毒gD蛋白基因的重组腺相关病毒转移载体、病毒及其制备和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001036651A1 (en) 1999-11-19 2001-05-25 Cantab Pharmaceuticals Research Limited Preparation of human or animal herpesviruses
CN101444622A (zh) * 2008-06-06 2009-06-03 中国人民解放军军事医学科学院军事兽医研究所 以猪伪狂犬病病毒为载体的日本血吸虫基因重组活疫苗生产工艺及制品

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4110962A1 (de) * 1991-04-05 1992-10-08 Bayer Ag Equine herpesviren (ehv), die fremd-dna enthalten, verfahren zu ihrer herstellung und ihre verwendung in impfstoffen
MX9206981A (es) * 1991-12-06 1994-08-31 Whitehead Biomedical Inst Vacunas de recombinantes y metodo para producirlas.
EP2468768A3 (en) * 2005-07-21 2012-10-31 Abbott Laboratories Multiple gene expression including sorf contructs and methods with polyproteins, pro-proteins, and proteolysis
BRPI0618441B8 (pt) * 2005-11-10 2021-07-27 Genvec Inc vetor adenoviral
RU2546247C2 (ru) 2013-06-06 2015-04-10 Автономная некоммерческая организация "Научно-исследовательский институт диагностики и профилактики болезней человека и животных" (АНО "НИИ ДПБ") Вакцина против чумы, аденовирусных инфекций, парвовирусного и коронавирусного энтеритов, лептоспироза и бешенства собак
MA42640B1 (fr) * 2015-11-23 2021-06-30 Merial Inc Protéines de fusion de dmdv et e2 et leurs utilisations
CN107723281B (zh) 2017-09-09 2021-02-12 中国农业科学院哈尔滨兽医研究所 表达猪圆环病毒2型Cap蛋白的重组猪瘟兔化弱毒疫苗株及其应用
EA202092164A1 (ru) * 2018-03-19 2021-02-10 Бёрингер Ингельхайм Ветмедика Гмбх Новый ehv с инактивированным ul18 и/или ul8
CA3094416A1 (en) * 2018-03-19 2019-09-26 Boehringer Ingelheim Vetmedica (China) Co., Ltd. Recombinant virus capable of stably expressing target proteins

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001036651A1 (en) 1999-11-19 2001-05-25 Cantab Pharmaceuticals Research Limited Preparation of human or animal herpesviruses
CN101444622A (zh) * 2008-06-06 2009-06-03 中国人民解放军军事医学科学院军事兽医研究所 以猪伪狂犬病病毒为载体的日本血吸虫基因重组活疫苗生产工艺及制品

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
VETERINARY MEDICINE, 2016, vol. 63, pages 30-41.*
VETERINARY RESEARCH, 2013, vol. 44:48.*

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Publication number Publication date
US11904010B2 (en) 2024-02-20
CN111902535A (zh) 2020-11-06
EP3768831A4 (en) 2022-01-05
MX2020009720A (es) 2021-01-08
AU2019237413B2 (en) 2025-08-07
AU2019237413A1 (en) 2020-09-24
US20210353734A1 (en) 2021-11-18
KR20200135412A (ko) 2020-12-02
CA3094416A1 (en) 2019-09-26
RU2020133457A (ru) 2022-04-19
CN111902535B (zh) 2024-08-27
WO2019179345A1 (en) 2019-09-26
EP3768831A1 (en) 2021-01-27
JP7387623B2 (ja) 2023-11-28
JP2021518152A (ja) 2021-08-02
AU2019237413C1 (en) 2025-10-30

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