KR102757122B1 - 1차 세포 샘플을 제조하는 방법 - Google Patents

1차 세포 샘플을 제조하는 방법 Download PDF

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KR102757122B1
KR102757122B1 KR1020187010765A KR20187010765A KR102757122B1 KR 102757122 B1 KR102757122 B1 KR 102757122B1 KR 1020187010765 A KR1020187010765 A KR 1020187010765A KR 20187010765 A KR20187010765 A KR 20187010765A KR 102757122 B1 KR102757122 B1 KR 102757122B1
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cell
cells
anoikis
inhibitor
sample
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KR20180057653A (ko
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란스 가빈 랭
벤 리치
아브히짓 단다팟
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셀퀴티 인크.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/48—Regulators of apoptosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/73—Hydrolases (EC 3.)
    • C12N2501/734—Proteases (EC 3.4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biochemistry (AREA)
  • Oncology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
KR1020187010765A 2015-10-20 2016-10-20 1차 세포 샘플을 제조하는 방법 Active KR102757122B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562243765P 2015-10-20 2015-10-20
US62/243,765 2015-10-20
PCT/US2016/057923 WO2017070353A1 (en) 2015-10-20 2016-10-20 Methods of preparing a primary cell sample

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KR20180057653A KR20180057653A (ko) 2018-05-30
KR102757122B1 true KR102757122B1 (ko) 2025-01-21

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US (2) US11591573B2 (cg-RX-API-DMAC7.html)
EP (1) EP3365436B1 (cg-RX-API-DMAC7.html)
JP (2) JP7202613B2 (cg-RX-API-DMAC7.html)
KR (1) KR102757122B1 (cg-RX-API-DMAC7.html)
CN (1) CN108138146A (cg-RX-API-DMAC7.html)
AU (1) AU2016341938B2 (cg-RX-API-DMAC7.html)
CA (1) CA2999054A1 (cg-RX-API-DMAC7.html)
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WO2017070353A1 (en) 2015-10-20 2017-04-27 Celcuity Llc Methods of preparing a primary cell sample
CN108300688B (zh) * 2018-02-07 2021-02-02 上海赛立维生物科技有限公司 原代肝细胞分离和培养方法
CN109402051A (zh) * 2018-12-28 2019-03-01 青岛麦迪赛斯生物科技有限公司 一种人脐带间充质干细胞无血清培养基
CN109777736B (zh) * 2019-01-29 2022-03-25 美闺(长沙)医疗美容有限公司 一种活细胞分析设备及方法
CN109943520B (zh) * 2019-03-08 2023-06-23 中国人民解放军军事科学院军事医学研究院 汗腺细胞的分离和培养获得汗腺类器官的方法及其应用
EP4073516A1 (en) 2019-12-09 2022-10-19 Celcuity Inc. Methods of treating cancer patients with ras node or rtk targeted therapeutic agents
CN111733136B (zh) * 2020-06-29 2021-11-30 中山大学孙逸仙纪念医院 一种提高CD90posi细胞分离效率的方法
US20260135422A1 (en) 2021-10-27 2026-05-14 Sumitomo Bakelite Co., Ltd. Stator and structure

Citations (2)

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WO2009066258A1 (en) 2007-11-20 2009-05-28 Hopitaux Universitaires De Geneve Methods for identifying, purifying and enriching immature or stem cancer-initiating cells from tumors and use thereof
WO2014132032A1 (en) 2013-02-27 2014-09-04 Cancer Research Technology Limited Culture of leukemia initiating cells

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SG47996A1 (en) 1993-12-30 1998-04-17 Nitta Gelatin Kk Method for culturing animal cells under embedded conditions
US5858678A (en) 1994-08-02 1999-01-12 St. Louis University Apoptosis-regulating proteins
US8252591B2 (en) 2004-05-07 2012-08-28 Whitehead Institute For Biomedical Research Hormone responsive tissue culture system and uses thereof
US20090130064A1 (en) 2004-10-27 2009-05-21 Vera Rogiers Differentiation of stem cells and stabilization of phenotypical properties of primary cells
FR2894594B1 (fr) * 2005-12-12 2008-02-08 Univ Limoges Test predictif de reponse therapeutique du cancer du sein
US9096827B2 (en) 2008-09-02 2015-08-04 Pluristem Ltd. Adherent cells from placenta tissue and use thereof in therapy
JP5774496B2 (ja) 2010-01-19 2015-09-09 株式会社ルネッサンス・エナジー・インベストメント 癌組織由来細胞塊または癌細胞凝集塊の培養方法、評価方法および保存方法
EP2566975A4 (en) 2010-05-03 2014-04-02 Microstem Inc SCREENING METHODS, COMPOSITIONS IDENTIFIED BY THIS METHOD, TOOLS USEFUL FOR THEIR IDENTIFICATION AND CELLULAR POPULATIONS THUS PRODUCED
TWI477599B (zh) 2011-01-28 2015-03-21 鴻海精密工業股份有限公司 培育基體
NO2764099T3 (cg-RX-API-DMAC7.html) * 2011-10-04 2018-04-21
US20130330761A1 (en) 2012-06-12 2013-12-12 Celcuity, LLC Whole cell assays and methods
US20140142000A1 (en) * 2012-10-25 2014-05-22 Academia Sinica Microfluidic array platform for simultaneous cell culture under oxygen tensions
CA3106271A1 (en) * 2013-01-25 2014-07-31 Xcell Biosciences, Inc. Methods, compositions, kits, and systems for selective enrichment of target cells
WO2015089380A2 (en) * 2013-12-12 2015-06-18 Celcuity Llc Assays and methods for determining the responsiveness of an individual subject to a therapeutic agent
WO2015156929A1 (en) * 2014-04-07 2015-10-15 The Trustees Of Columbia University In The City Of New York Method for culture of human bladder cell lines and organoids and uses thereof
WO2017070353A1 (en) 2015-10-20 2017-04-27 Celcuity Llc Methods of preparing a primary cell sample

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
WO2009066258A1 (en) 2007-11-20 2009-05-28 Hopitaux Universitaires De Geneve Methods for identifying, purifying and enriching immature or stem cancer-initiating cells from tumors and use thereof
WO2014132032A1 (en) 2013-02-27 2014-09-04 Cancer Research Technology Limited Culture of leukemia initiating cells

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EP3365436B1 (en) 2021-09-08
DK3365436T3 (da) 2021-10-11
JP2018531021A (ja) 2018-10-25
KR20180057653A (ko) 2018-05-30
AU2016341938B2 (en) 2022-12-01
WO2017070353A1 (en) 2017-04-27
US20180230434A1 (en) 2018-08-16
JP7202613B2 (ja) 2023-01-12
EP3365436A1 (en) 2018-08-29
CN108138146A (zh) 2018-06-08
US12215356B2 (en) 2025-02-04
CA2999054A1 (en) 2017-04-27
JP2021175415A (ja) 2021-11-04
HK1252635A1 (zh) 2019-05-31
US20230235293A1 (en) 2023-07-27
US11591573B2 (en) 2023-02-28
AU2016341938A1 (en) 2018-04-26

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