KR102711998B1 - 조작되고 완전-기능 맞춤 당단백질 - Google Patents

조작되고 완전-기능 맞춤 당단백질 Download PDF

Info

Publication number
KR102711998B1
KR102711998B1 KR1020207002825A KR20207002825A KR102711998B1 KR 102711998 B1 KR102711998 B1 KR 102711998B1 KR 1020207002825 A KR1020207002825 A KR 1020207002825A KR 20207002825 A KR20207002825 A KR 20207002825A KR 102711998 B1 KR102711998 B1 KR 102711998B1
Authority
KR
South Korea
Prior art keywords
host cell
sequence
delete delete
sialyltransferase
leishmania
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020207002825A
Other languages
English (en)
Korean (ko)
Other versions
KR20200022486A (ko
Inventor
아밀레자 파리드모아이어
마누엘라 말리
라이너 폴라도르
Original Assignee
림마테크 바이오로직스 아게
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 림마테크 바이오로직스 아게 filed Critical 림마테크 바이오로직스 아게
Priority to KR1020247032085A priority Critical patent/KR20240145084A/ko
Publication of KR20200022486A publication Critical patent/KR20200022486A/ko
Application granted granted Critical
Publication of KR102711998B1 publication Critical patent/KR102711998B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/44—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from protozoa
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/10—Protozoa; Culture media therefor
    • C12N1/105—Protozoal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/05—Fusion polypeptide containing a localisation/targetting motif containing a GOLGI retention signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/90—Protozoa ; Processes using protozoa
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01003—Glucosamine N-acetyltransferase (2.3.1.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01022—Lactose synthase (2.4.1.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01101—Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.101)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01143—Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (2.4.1.143)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C12Y204/99006—N-Acetyllactosaminide alpha-2,3-sialyltransferase (2.4.99.6)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1020207002825A 2017-06-30 2018-06-28 조작되고 완전-기능 맞춤 당단백질 Active KR102711998B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020247032085A KR20240145084A (ko) 2017-06-30 2018-06-28 조작되고 완전-기능 맞춤 당단백질

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762527466P 2017-06-30 2017-06-30
US62/527,466 2017-06-30
PCT/EP2018/067494 WO2019002512A2 (en) 2017-06-30 2018-06-28 GLYCOPROTEINS CUSTOMIZED FROM ENGINEERING AND FULLY FUNCTIONAL

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020247032085A Division KR20240145084A (ko) 2017-06-30 2018-06-28 조작되고 완전-기능 맞춤 당단백질

Publications (2)

Publication Number Publication Date
KR20200022486A KR20200022486A (ko) 2020-03-03
KR102711998B1 true KR102711998B1 (ko) 2024-10-04

Family

ID=62815031

Family Applications (2)

Application Number Title Priority Date Filing Date
KR1020207002825A Active KR102711998B1 (ko) 2017-06-30 2018-06-28 조작되고 완전-기능 맞춤 당단백질
KR1020247032085A Pending KR20240145084A (ko) 2017-06-30 2018-06-28 조작되고 완전-기능 맞춤 당단백질

Family Applications After (1)

Application Number Title Priority Date Filing Date
KR1020247032085A Pending KR20240145084A (ko) 2017-06-30 2018-06-28 조작되고 완전-기능 맞춤 당단백질

Country Status (7)

Country Link
US (2) US11913051B2 (https=)
EP (1) EP3645732A2 (https=)
JP (2) JP7437162B2 (https=)
KR (2) KR102711998B1 (https=)
CN (3) CN119639577A (https=)
AU (1) AU2018294504B2 (https=)
WO (1) WO2019002512A2 (https=)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019002512A2 (en) 2017-06-30 2019-01-03 Limmatech Biologics Ag GLYCOPROTEINS CUSTOMIZED FROM ENGINEERING AND FULLY FUNCTIONAL
WO2019234021A1 (en) 2018-06-04 2019-12-12 Limmatech Biologics Ag Glycoengineered monoclonal antibody
CN111518736B (zh) 2019-10-24 2022-04-15 华熙生物科技股份有限公司 一种高效合成高纯度透明质酸及其寡聚糖的重组谷氨酸棒状杆菌
WO2021140143A1 (en) 2020-01-07 2021-07-15 Limmatech Biologics Ag Glycoengineering using leishmania cells
CA3166728A1 (en) * 2020-01-07 2021-07-15 Limmatech Biologics Ag Engineered leishmania cells
CA3181572A1 (en) * 2020-06-08 2021-12-16 Paul STABACH Compositions and methods for treating and/or preventing coagulopathy and/or sepsis in patients suffering from bacterial and/or viral infections
WO2022053673A1 (en) 2020-09-14 2022-03-17 Limmatech Biologics Ag Fab sialylation of antibodies
US20250281626A1 (en) 2021-03-23 2025-09-11 Glycoera Ag Mannose 3 glycan-mediated protein degradation
AU2022246021A1 (en) 2021-03-23 2023-10-05 Glycoera Ag Glycan-mediated protein degradation
EP4330383A1 (en) * 2021-04-29 2024-03-06 Novartis AG Hypersialylating cells
JP2025534987A (ja) 2022-09-28 2025-10-22 グリコエラ アーゲー グリカン媒介タンパク質分解
AU2024206743A1 (en) 2023-01-05 2025-08-14 Glycoera Ag Glycoengineered polypeptides targeting anti-neutrophil autoantibodies and uses thereof
WO2024147113A1 (en) 2023-01-05 2024-07-11 Glycoera Ag Glycoengineered polypeptides targeting anti-podocyte autoantibodies and uses thereof
US20260055191A1 (en) 2023-01-05 2026-02-26 Glycoera Ag Glycoengineered polypeptides targeting immunoglobulin a and complexes comprising the same
WO2024218743A1 (en) 2023-04-21 2024-10-24 Glycoera Ag Multi-functional molecules comprising glycans and uses thereof
WO2025215516A1 (en) 2024-04-08 2025-10-16 Glycoera Ag Glycoengineered polypeptides targeting igg4 autoantibodies and uses thereof
WO2025226309A1 (en) * 2024-04-26 2025-10-30 Rock Biomedical Inc. Engineered glycoprotein population and uses thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009536031A (ja) * 2006-05-05 2009-10-08 グライコフィ， インコーポレイテッド 下等真核生物におけるシアル酸付加ｎ−グリカンの産生
US20110287481A1 (en) 2008-10-01 2011-11-24 Asahi Glass Company, Limited Host, transformant and method for producing the transformant and method for producing o-glycosylated heterologous protein

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2388151C (en) * 1999-11-05 2010-01-12 Jena Bioscience Gmbh Protein expression systems for non-pathogenic kinetoplastidae
CN107119095B (zh) * 2008-01-03 2022-07-05 康乃尔研究基金会有限公司 原核生物中的糖基化蛋白表达
JP2015502144A (ja) * 2011-10-28 2015-01-22 メルク・シャープ・アンド・ドーム・コーポレーションＭｅｒｃｋ Ｓｈａｒｐ ＆ Ｄｏｈｍｅ Ｃｏｒｐ． Ａｌｇ３の発現を欠損したピキア・パストリス（Ｐｉｃｈｉａｐａｓｔｒｉｓ）株中のＮ−グリカン占有率を増加させ、ハイブリッドＮ−グリカンの産生を低減させる方法
CA3006400A1 (en) 2015-11-30 2017-06-08 Limmatech Biologics Ag Methods of producing glycosylated proteins
WO2019002512A2 (en) 2017-06-30 2019-01-03 Limmatech Biologics Ag GLYCOPROTEINS CUSTOMIZED FROM ENGINEERING AND FULLY FUNCTIONAL
WO2021140143A1 (en) 2020-01-07 2021-07-15 Limmatech Biologics Ag Glycoengineering using leishmania cells
CA3166728A1 (en) 2020-01-07 2021-07-15 Limmatech Biologics Ag Engineered leishmania cells
WO2022053673A1 (en) 2020-09-14 2022-03-17 Limmatech Biologics Ag Fab sialylation of antibodies

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009536031A (ja) * 2006-05-05 2009-10-08 グライコフィ， インコーポレイテッド 下等真核生物におけるシアル酸付加ｎ−グリカンの産生
US20110287481A1 (en) 2008-10-01 2011-11-24 Asahi Glass Company, Limited Host, transformant and method for producing the transformant and method for producing o-glycosylated heterologous protein

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Amjad Hayat Khan et al, "Humanizing glycosylation pathways in eukaryotic expression systems", World J. Microbiol. Biotechnol., 2017, 33(1):4*

Also Published As

Publication number Publication date
CN111108211A (zh) 2020-05-05
KR20240145084A (ko) 2024-10-04
JP2020530273A (ja) 2020-10-22
AU2018294504A1 (en) 2020-01-23
CN117487873A (zh) 2024-02-02
JP2023134519A (ja) 2023-09-27
KR20200022486A (ko) 2020-03-03
US20210332403A1 (en) 2021-10-28
US20240287568A1 (en) 2024-08-29
US11913051B2 (en) 2024-02-27
JP7437162B2 (ja) 2024-02-22
WO2019002512A3 (en) 2019-02-28
AU2018294504B2 (en) 2024-07-11
EP3645732A2 (en) 2020-05-06
WO2019002512A2 (en) 2019-01-03
CN119639577A (zh) 2025-03-18

Similar Documents

Publication Publication Date Title
KR102711998B1 (ko) 조작되고 완전-기능 맞춤 당단백질
KR102700050B1 (ko) 조작된 내수송/외수송을 가진 미생물 숙주에서 모유 올리고당류의 생산
KR102319845B1 (ko) 조류 숙주 세포에 대한 crispr-cas 시스템
KR102494564B1 (ko) 말라리아 백신
DK2087105T3 (da) Delta 17-desaturase og anvendelse heraf ved fremstilling af flerumættede fedtsyrer
KR20220012327A (ko) 피토칸나비노이드 및 피토칸나비노이드 전구체의 생산을 위한 방법 및 세포
KR20070085649A (ko) 고농도의 에이코사펜타엔산을 생성하는 야로위아리폴리티카 균주
DK2623594T3 (da) Antistof mod human prostaglandin-E2-receptor EP4
BRPI0817233A2 (pt) construções terapêuticas de gene de trca e bireatores para a expressão de moléculas bioterapêuticas, e usos das mesmas
KR20210105382A (ko) 단백질을 코딩하는 rna
KR20220121844A (ko) 유전자의 발현을 동시에 조절하기 위한 조성물 및 방법
CN109996874A (zh) 10-甲基硬脂酸的异源性产生
AU2017233862B2 (en) Methods and compositions for increased double stranded RNA production
AU2017252409B2 (en) Compositions and methods for nucleic acid expression and protein secretion in bacteroides
KR20240022571A (ko) Rna-가이드된 이펙터 동원을 위한 시스템, 방법 및 성분
KR20230134524A (ko) 무장된 세네카 밸리 바이러스 종양용해 요법 조성물및 그의 방법
US11814412B2 (en) Artificial proteins and compositions and methods thereof
RU2798786C2 (ru) Производство молочных олигосахаридов человека в микробных продуцентах с искусственным импортом/экспортом
KR102721142B1 (ko) 재배열 레오바이러스과 바이러스를 제조하는 방법 및 이를 위한 벡터 라이브러리
US20240165154A1 (en) Methods and agents for modulating adoptive immunotherapy
CN112209883B (zh) 一种与rna特异性结合的荧光素染料及其应用
RU2774631C1 (ru) Сконструированные компоненты cascade и комплексы cascade
RU2827658C2 (ru) Сконструированные компоненты cascade и комплексы cascade
HK40091834A (zh) 增加双链rna产生的方法和组合物
KR20240023100A (ko) 유전자 발현을 조절하기 위한 조성물 및 방법

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20200129

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20210624

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20230516

Patent event code: PE09021S01D

PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20240626

A107 Divisional application of patent
GRNT Written decision to grant
PA0104 Divisional application for international application

Comment text: Divisional Application for International Patent

Patent event code: PA01041R01D

Patent event date: 20240925

PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20240925

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20240926

End annual number: 3

Start annual number: 1

PG1601 Publication of registration