KR102709570B1 - Cosmetic composition comprising probiotics cultivatied using hot spring water and plant complex extract as active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising, as active ingredients, a culture comprising a Lactobacillus genus strain cultured in a medium containing hot spring water; a culture comprising a Bifidobacterium genus strain cultured in a medium containing Dogo hot spring water; and a plant complex extract comprising an Amaranthus lividus leaf extract, a Digitaria ciliaris stem extract, and a Polygonum aviculare L. extract. The cosmetic composition of the present invention inhibits the production of MMP-1 protein and promotes collagen production, and has the ability to inhibit tyrosinase and melanin production, thereby exhibiting excellent skin whitening, wrinkle formation inhibition, and wrinkle improvement effects.

Description

{COSMETIC COMPOSITION COMPRISING PROBIOTICS CULTIVATIED USING HOT SPRING WATER AND PLANT COMPLEX EXTRACT AS ACTIVE INGREDIENT}

The present invention relates to a cosmetic composition comprising probiotics and plant complex extracts cultured using hot spring water.

The skin is the largest organ in the body and is always in direct contact with the external environment, and acts as a protective layer to protect the body from various stimuli and dry environments. Compared to other organs, it actively produces and destroys new cells. In addition, it protects the body from physical abrasions, prevents moisture loss inside the body, protects against ultraviolet rays from the sun, and plays a very important role in regulating the body's temperature.

This skin suffers from skin contamination, dryness, troubles, and skin immune system abnormalities due to various physical factors, external environmental factors, and infections. In particular, with the rapid development of modern industry, air pollution, water pollution, and other pollution problems have become serious, and the skin is greatly affected by the external environment, such as roughening, dryness, and aging, due to strong heating and cooling in living spaces due to the improvement of living environments. Human skin gradually becomes thinner, wrinkles increase, and elasticity decreases due to intrinsic aging and photoaging, and freckles and blemishes increase due to exposure to ultraviolet rays. In particular, intrinsic aging occurs when the biosynthesis of biological components decreases due to a decrease in hormone secretion or a decline in the function and activity of immune cells, and this progresses rapidly due to an increase in active oxygen.

Humans constantly generate reactive oxygen species during their metabolic processes, and it is known that these act as direct and indirect causes of aging. Various antioxidants are used in cosmetics to prevent skin aging, and representative synthetic antioxidants include BHT (butylatedhydroxytoluene), BHA (butylated hydroxyanisole), and tertiary butylhydroquinone (TBHQ). In general, synthetic antioxidants are widely used due to their excellent effects and low price, but their use is restricted because they are known to cause not only their own toxicity but also hepatomegaly, fatty liver, and cancer when taken orally or in contact with the skin.

Niacinamide and arbutin, which are used as raw materials for whitening functional cosmetics, and retinol, which is used as raw materials for wrinkle-improving functional cosmetics, have problems with their whitening effects being reduced due to the destruction of ingredients by oxidation and the limitation of the mixing amount caused by skin irritation. Recently, as various cosmetic compositions using natural substances have been released on the market, consumers are becoming more interested in products using natural substances than in existing products made of chemical substances, and accordingly, various cosmetic compositions using natural substances are being manufactured.

In addition, the wrinkle improvement effect by cosmetics is due to the effect of improving the dermis layer by various effective ingredients in addition to the temporary effect of moisturizing by moisturizing factors, and it is well known that growth factor ingredients such as retinol, vitamin C, and EGF, which are representative raw materials of wrinkle improvement cosmetics, show wrinkle improvement effect by increasing collagen production from human dermal fibroblasts. In order to prevent this phenomenon and maintain healthier and more beautiful skin, efforts have been made to maintain the skin's inherent function and activate skin cells by adding and using physiologically active substances obtained from various animals, plants, and microorganisms that are known to be present in cosmetics, thereby effectively inhibiting skin aging.

In the cosmetics field, research and development on functional cosmetics with whitening and wrinkle improvement effects are actively being conducted, and in particular, research using natural substances is continuing to avoid causing toxicity or irritation to the skin.

Korean Patent Registration No. 10-1991830 Korean Patent No. 10-1405429

The purpose of the present invention is to provide a cosmetic composition having skin whitening, wrinkle suppression or wrinkle improvement effects by using a probiotic culture cultured using hot spring water and plant components commonly considered as weeds.

According to one aspect of the present invention,

A cosmetic composition is provided, which comprises, as active ingredients, a culture comprising a Lactobacillus genus strain cultured in a medium containing hot spring water; a culture comprising a Bifidobacterium genus strain cultured in a medium containing hot spring water; and a plant complex extract comprising an Amaranthus lividus leaf extract, a Digitaria ciliaris stem extract, and a Polygonum aviculare L. extract.

The above plant complex extract may additionally include a Solanum nigrum leaf extract.

The above plant complex extract can be extracted with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

The medium containing the above hot spring water can contain 10 to 40% (v/v) of hot spring water.

In the above cosmetic composition, the plant complex extract may be included in an amount of 50 to 70% (v/v).

In the above cosmetic composition, the culture containing the Lactobacillus genus strain and the culture containing the Bifidobacterium genus strain may be included in a volume ratio of 10:5 to 10:15.

The above Lactobacillus strain may be any one selected from Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus harbinensis, Lactobacillus casei, Lactobacillus sakei, Lactobacillus brevis, Lactobacillus pentosus, Lactobacillus fermentum, Lactobacillus acidophilus, and Lactobacillus rhamnosus.

The above Bifidobacterium genus strain may be any one selected from Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium lactis, and Bifidobacterium bifidum.

The above cosmetic composition may be used for skin whitening, wrinkle suppression or wrinkle improvement.

The cosmetic composition containing probiotics cultured using the hot spring water of the present invention and plant complex extract inhibits the production of MMP-1 protein, promotes collagen production, and has the ability to inhibit tyrosinase and melanin production, so it has excellent skin whitening and wrinkle improvement effects. In addition, by using weeds recognized as dandelion, barley, knotweed, and black sorrel as raw materials for the plant complex extract, the supply of raw materials is easy, the cost is reduced, and the useful functional ingredients of discarded weeds can be utilized.

The present invention can be modified in various ways and has various embodiments, and specific embodiments are illustrated in the drawings and described in detail in the detailed description. However, this is not intended to limit the present invention to specific embodiments, and it should be understood that it includes all modifications, equivalents, and substitutes included in the spirit and technical scope of the present invention. In describing the present invention, if it is determined that a specific description of a related known technology may obscure the gist of the present invention, the detailed description thereof will be omitted.

Hereinafter, a cosmetic composition containing probiotics and plant complex extracts cultured using the hot spring water of the present invention will be described.

The cosmetic composition of the present invention is characterized by including, as active ingredients, a culture comprising a Lactobacillus genus strain cultured in a medium containing hot spring water; a culture comprising a Bifidobacterium genus strain cultured in a medium containing hot spring water; and a plant complex extract comprising an Amaranthus lividus leaf extract, a Digitaria ciliaris stem extract, and a Polygonum aviculare extract.

It is preferable that the culture containing the Lactobacillus genus strain and the culture containing the Bifidobacterium genus strain are included in a crushed state without removing the cultured strain.

Preferably, the plant complex extract may additionally include Solanum nigrum leaf extract. By including Solanum nigrum leaf extract, the whitening functionality and wrinkle improvement effect can be further improved.

The above plant complex extract may be extracted by water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, and preferably may be a hot water extract.

Amaranthus lividus is an annual plant of the Amaranthaceae family. It is known to be native to Europe, and its stem is weak and its height is about 25-80cm. The base is on the ground or stands obliquely, and the branches are many-branched. The leaves are diamond-shaped and egg-shaped, arranged alternately, and the tips are concave. They are 4-8cm long and 2-4cm wide, and have long petioles. From July to November, small green flowers bloom in small spikes at the leaf axils or branches. It has three perianth segments and stamens, one pistil, and the fruit is egg-shaped, slightly wrinkled, and does not burst. The seeds are about 1mm in diameter and dark brown. It is a weed commonly seen in fields, rice paddies, and roadsides.

Digitaria ciliaris is an annual grass of the gramineae family and a weed that is widespread throughout the world. It commonly grows in fields, ridges, and roadsides, and as it creeps on the ground, new roots grow from the nodes at the bottom of the stem and spread very quickly. The upper part of the stem is straight and it is about 30–70 cm tall. The leaves that grow at the bottom of the stem are about 8–20 cm long and 5–15 mm wide and have hairs. The inflorescence is very thin and straight, about 4–8 mm long, and branches out into 3–8 branches from the stem. The inflorescence is reddish or purple.

Polygonum aviculare is an annual herb distributed in temperate and subtropical regions of the Northern Hemisphere. It is hairless throughout, the stem branches out and grows upward, has many vertical lines, and the nodes are swollen. It is about 10–40 cm tall. The leaves have short petioles, are opposite, long-ovate, 2–4 cm long, hairless, and the back has a prominent central vein. The leaf sheath is membranous, irregularly torn, and has thin veins. From July to September, small green flowers bloom in clusters in the leaf axils. The perianth has five lobes, the margins are white or pink, and the fruit is an achene with fine wrinkles and is covered with perianth lobes.

Solanum nigrum is an annual plant of the Solanaceae family, widely distributed in Korea and temperate and tropical regions. It is 20–90 cm tall, has many branches that spread out, and the main stem has some ridges. The leaves are opposite, ovate, and have smooth or wavy serrations at the margins. The flowers bloom from May to July, are white, and are 6–7 mm in diameter. The calyx is divided into five lobes, the corolla also spreads out and is divided into five lobes, and there is one pistil and five stamens. The fruit is a round berry that is black when fully ripe and sweet, but caution is required as it contains the slightly toxic solanine.

In the above cosmetic composition, it is preferable that the plant complex extract is contained in an amount of 50 to 70% (v/v), more preferably 55 to 65% (v/v). If it is out of the above range, the skin whitening and wrinkle improvement effects may be reduced.

In the above cosmetic composition, the culture containing the Lactobacillus genus strain and the culture containing the Bifidobacterium genus strain are preferably in a volume ratio of 10:5 to 10:15, more preferably in a volume ratio of 10:7 to 10:13, even more preferably in a volume ratio of 10:9 to 10:11, and most preferably in a volume ratio of 1:1. If it is out of the above range, the skin whitening and wrinkle improvement effects may be reduced.

The medium containing the above-mentioned hot spring water preferably contains 10 to 40% (v/v) of the above-mentioned hot spring water, and more preferably 25 to 35% (v/v). If it is less than 10% (v/v), the wrinkle improvement or whitening effect may be reduced, and if it exceeds 40% (v/v), it may be difficult to culture probiotics.

The above Lactobacillus strain is preferably one selected from Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus harbinensis, Lactobacillus casei, Lactobacillus sakei, Lactobacillus brevis, Lactobacillus pentosus, Lactobacillus fermentum, Lactobacillus acidophilus, and Lactobacillus rhamnosus, and more preferably Lactobacillus plantarum. plantarum).

The above Lactobacillus plantarum is a bacterium that produces metabolites useful to the human body and is safe enough to be used as a food raw material. The Lactobacillus plantarum culture extract of the present invention is obtained by culturing the Lactobacillus plantarum and disrupting the cells with a homogenizer, and is a fermented lysate containing metabolic components, cytoplasm, cell wall components, polysaccharides, etc. of Lactobacillus plantarum.

In addition, the Bifidobacterium genus strain is preferably one selected from Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium lactis, and Bifidobacterium bifidum, and more preferably Bifidobacterium longum.

Bifidobacterium longum is a gram-positive lactic acid bacterium that exists in the human gastrointestinal tract. It has been isolated from the human body and its safety has been proven. It is also known as a beneficial bacterium that improves the intestinal environment.

The above hot spring water is characterized by containing 55 to 65 mg/L of calcium (Ca), 25 to 35 mg/L of sodium (Na), 10 to 20 mg/L of silicon (Si), 0.001 to 0.05 mg/L of lithium (Li), and 1.0 to 2.0 mg/L of strontium (Sr).

The cosmetic composition of the present invention is characterized by being for skin whitening, wrinkle suppression or wrinkle improvement.

The term “extract” as used herein includes not only a crude extract obtained by treating an extraction solvent, but also a processed product of the extract. For example, the extract may be prepared into a powder form by additional processes such as distillation under reduced pressure and freeze drying or spray drying.

In addition, the plant complex extract of the present invention has a broad meaning including a formulated processed product, such as a plant complex extract powder. Although the present invention was conducted with a plant complex extract, it would be expected by those skilled in the art that the desired effect can be achieved even in a form such as a processed plant complex extract.

Meanwhile, the term “comprising as an effective ingredient” in this specification means including a sufficient amount to achieve the efficacy or activity of the component including the probiotic culture. For example, the probiotic culture is used at a concentration of 10 to 1500 ㎍/㎖, preferably 100 to 1000 ㎍/㎖. Since the probiotic culture is a natural product and does not cause side effects to the human body even when applied excessively to the skin, the quantitative upper limit of the probiotic culture included in the composition of the present invention can be selected and implemented within an appropriate range by those skilled in the art.

The term "skin whitening" used in the present invention refers to whitening the skin. Human skin color is determined by the concentration and distribution of melanin inside the skin, and is affected by environmental or physiological conditions such as ultraviolet rays from the sun, fatigue, and stress in addition to genetic factors. Melanin is synthesized in melanocytes in the epidermal layer of the skin. In the melanosome, an organelle inside the melanocyte, tyrosine, an amino acid, is changed into DOPA and dopaquinone by the action of an enzyme called tyrosinase, which then undergoes a non-enzymatic oxidation reaction to create melanin. If such melanin synthesis occurs excessively inside the skin, it darkens the skin tone and causes blemishes and freckles. Therefore, by inhibiting the activity of tyrosinase in the skin and thus inhibiting the synthesis of melanin pigment, it is possible to achieve skin whitening by brightening the skin tone, as well as improve skin hyperpigmentation such as freckles and blemishes caused by ultraviolet rays, hormones, and genetic causes.

In addition, the term "wrinkle" in the present invention means fine lines formed when the skin weakens, and can be caused by genetic causes, a decrease in collagen existing in the dermis of the skin, external environments, etc. In the present invention, "wrinkle improvement" means suppressing or inhibiting the formation of wrinkles on the skin, or alleviating wrinkles that have already formed.

The main functions of skin fibroblasts include regeneration of damaged skin tissue through extracellular matrix synthesis and proliferation, but extrinsic aging factors such as photoaging, accumulated damage by free oxygen, and intrinsic aging factors such as cell aging due to telomere severing decrease the physiological activity of skin fibroblasts in the dermal layer. When the synthesis of type I collagen, which accounts for more than 95% of the skin extracellular matrix and plays a very important role in the physiological activity of fibroblasts through interaction and signal exchange with cell adhesion molecules and cytoskeleton, is reduced, the amount of collagen in skin tissue decreases, which reduces surface tension, lowers skin elasticity, forms skin wrinkles, and causes a vicious cycle in which physiological activity including cell proliferation and regeneration functions decreases.

Therefore, it can be seen that collagen of skin fibroblasts is directly related to skin regeneration, skin elasticity, skin wrinkle formation, and tissue repair or regeneration when skin is damaged. That is, if the synthesis of collagen or procollagen of skin fibroblasts is promoted, or the synthesis of matrix metalloproteinase (hereinafter referred to as 'MMP') that decomposes collagen is inhibited, effects such as improved skin elasticity, skin regeneration, improved skin wrinkles, wound healing, repair and regeneration of damaged skin tissue, and prevention of skin aging can be obtained.

The cosmetic composition of the present invention is characterized by increasing the expression of type I procollagen protein and inhibiting the expression of MMP-1 protein. Specifically, the cosmetic composition of the present invention increases the production of type I procollagen while inhibiting the expression of matrix metalloproteinase-1, which promotes collagen decomposition, and can have effects such as increasing skin elasticity, skin regeneration, improving skin wrinkles, wound healing, repairing and regenerating damaged skin tissue, and preventing skin aging. That is, it can have the effect of preventing or improving skin wrinkles.

In addition, the cosmetic composition of the present invention may further include water-soluble vitamins, fat-soluble vitamins, high molecular peptides, high molecular polysaccharides, sphingolipids, and seaweed extract.

The water-soluble vitamins mentioned above may be any that can be mixed in cosmetics, but preferably include vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinamide, folic acid, vitamin C, vitamin H, etc., and their salts (thiamine hydrochloride, sodium ascorbate, etc.) or derivatives (sodium ascorbate-2-phosphate, magnesium ascorbate-2-phosphate, etc.) are also included in the water-soluble vitamins that can be used in the present invention. The water-soluble vitamins can be obtained by a conventional method such as a microbial transformation method, a microbial culture purification method, an enzymatic method, or a chemical synthesis method.

The fat-soluble vitamins mentioned above may be any that can be incorporated into cosmetics, but preferably include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E, etc., and their derivatives (ascorbic acid palmitate, ascorbic acid stearate, ascorbic acid dipalmitate, dl-alpha tocopheryl acetate, dl-alpha tocopheryl nicotinate, vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether, etc.) are also included in the fat-soluble vitamins used in the present invention.

The polymer peptide may be any polymer that can be incorporated into cosmetics, but preferably includes collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, etc. The polymer peptide may be purified and obtained by a conventional method such as a purification method of a microbial culture solution, an enzymatic method, or a chemical synthesis method, or may be purified and used from natural products such as the dermis of pigs or cows, or silkworm fibers.

The above polymer polysaccharide may be any polymer that can be mixed in cosmetics, but preferably, hydroxyethyl cellulose, xanthan gum, chondroitin sulfate or a salt thereof (sodium salt, etc.) may be used. For example, chondroitin sulfate or a salt thereof can usually be purified from mammals or fish and used.

The sphingolipid may be any one that can be incorporated into cosmetics, but preferably includes ceramide, phytosphingosine, sphingosaccharide lipid, etc. The sphingolipid may be purified by a conventional method from mammals, fish, shellfish, yeast, or plants, or may be obtained by a chemical synthesis method.

The above seaweed extract may be any one that can be mixed in cosmetics, but preferably, brown seaweed extract, red seaweed extract, green seaweed extract, etc. are used. In addition, calagenan, arginic acid, sodium arginate, potassium arginate, etc. purified from these seaweed extracts are also included in the seaweed extract used in the present invention. The seaweed extract can be obtained by purifying seaweed by a conventional method.

In addition to the above essential ingredients, the cosmetic composition of the present invention may contain other ingredients commonly incorporated in cosmetics as needed.

Other ingredients that may be added include fat-soluble ingredients, moisturizers, emollients, UV absorbers, pH adjusters, fragrances, circulation promoters, cooling agents, antiperspirants, and purified water.

The above maintenance ingredients include ester-based fats, hydrocarbon-based fats, silicone-based fats, fluorine-based fats, animal fats, and plant fats.

The above ester-based fats and oils include tri-2-ethylhexanoate glyceryl, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, diisopropyl adipate, isoalkyl neopentanoate, tri(caprylic, capric)glyceryl, trimethylolpropane tri-2-ethylhexanoate, trimethylolpropane triisostearate, tetra-2-ethylhexanoate pentaellistolate, cetyl caprylate, decyl laurate, Hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isostearyl laurate, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isostearate isopropyl, cetostearyl 2-ethylhexanoate, stearyl 2-ethylhexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, Triisostearate glyceryl, neopentanoate octyldodecyl, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerol oleate ester, polyglycerol isostearate ester, triisocetyl citrate, triisoalkyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, Examples thereof include esters such as diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesterol stearate, cholesterol isostearate, cholesterol hydroxystearate, cholesterol oleate, dehydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, isocetyl 12-stearoylhydroxystearate, stearyl 12-stearoylhydroxystearate, and isostearyl 12-stearoylhydroxystearate.

Examples of the hydrocarbon-based fats and oils include squalene, liquid paraffin, alpha-olefin oligomers, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybdenum, microcrystalline wax, and petrolatum.

Examples of the silicone-based oils include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane methylcetyloxysiloxane copolymer, dimethylsiloxane methyl stearoxysiloxane copolymer, alkyl-modified silicone oil, and amino-modified silicone oil.

Examples of the above fluorine-based oils include perfluoropolyether.

The above animal or plant fats include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil, cottonseed oil, palm oil, kukui nut oil, wheat germ oil, rice germ oil, shea butter, moonflower oil, marc damiana nut oil, egg yolk oil, beef tallow, horse oil, mink oil, orange rye oil, jojoba oil, candelilla wax, carnauba wax, liquid lanol, hydrogenated castor oil, and the like.

The above-mentioned moisturizers include water-soluble low-molecular-weight moisturizers, fat-soluble molecular-weight moisturizers, water-soluble polymers, and fat-soluble polymers.

Examples of the water-soluble low-molecular-weight moisturizers include serine, glutamine, sorbitol, mannitol, sodium pyrrolidone-carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or higher), polypropylene glycol (polymerization degree n = 2 or higher), polyglycerin B (polymerization degree n = 2 or higher), lactic acid, and lactate salts.

The above-mentioned lipid-soluble low-molecular-weight moisturizers include cholesterol, cholesterol esters, etc.

Examples of the water-soluble polymers include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc.

Examples of the above-mentioned lipid-soluble polymers include polyvinylpyrrolidone eicosene copolymer, polyvinylpyrrolidone hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the above emollients include long-chain acylglutamic acid cholesteryl ester, hydroxystearate cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester.

The above ultraviolet absorbers include para-aminobenzoic acid, para-aminobenzoic acid ethyl, para-aminobenzoic acid amyl, para-aminobenzoic acid octyl, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicylate, butylphenyl salicylate, homomenthyl salicylate, benzyl cinnamic acid, para-methoxycinnamic acid-2-ethoxyethyl, para-methoxycinnamic acid octyl, dipara-methoxycinnamic acid mono-2-ethylhexaneglyceryl, para-methoxycinnamic acid isopropyl, diisopropyl-diisopropyl cinnamic acid ester mixture, urocanic acid, ethyl urocanic acid, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxybenzophenone, tetrahydroxybenzophenone, Examples include 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy)-1,3,5-triazine, and 2-(2-hydroxy-5-methylphenyl)benzotriazole.

The above pH adjusters include citric acid, sodium citrate, malic acid, sodium malate, formaldehyde, sodium formaldehyde, succinic acid, sodium succinate, sodium hydroxide, sodium hydrogen phosphate, etc.

The above pH adjusters include citric acid, sodium citrate, malic acid, sodium malate, formaldehyde, sodium formaldehyde, succinic acid, sodium succinate, sodium hydroxide, sodium hydrogen phosphate, etc.

A cosmetic manufactured by containing the cosmetic composition of the present invention may take the form of a solution, an emulsion, a viscous mixture, etc.

In addition, the components included in the cosmetic composition of the present invention may include components commonly used in cosmetic compositions in addition to the above-mentioned components as effective ingredients, and may further include conventional auxiliary agents and carriers such as stabilizers, pigments, and natural fragrances.

The cosmetic composition of the present invention can be used in various ways, such as in cosmetics or cleansers having skin whitening or wrinkle improvement effects.

Products to which the cosmetic composition of the present invention can be added include, for example, cosmetics such as BB cream, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, UV protection cream, moisture cream, hand cream, foundation, essence, nutrition essence, mask pack, and soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide can be used as a carrier component.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder can be used as a carrier component, and particularly in the case of a spray, a propellant such as chlorofluorohydrocarbon, propane, butane or dimethyl ether can be additionally included.

When the formulation of the present invention is a solution or emulsion, a solvent, a solvating agent or an emulsifying agent is used as a carrier component, and examples thereof include water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, liquid diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, etc. can be used as carrier components.

Hereinafter, preferred examples are presented to help understand the present invention, but the following examples are only illustrative of the present invention, and it will be apparent to those skilled in the art that various changes and modifications are possible within the scope and technical idea of the present invention, and it is natural that such changes and modifications fall within the scope of the appended patent claims.

[Example]

Manufacturing Example A: Extract of Gaebireum leaf

The leaves of Amaranthus lividus were finely chopped, extracted with hot water at 100°C for 4 hours in a ratio of 1:10 (w/v) with water, filtered through a non-woven fabric to remove impurities, and concentrated under reduced pressure to a solid content of 15% or more to prepare an Amaranthus lividus extract.

Manufacturing Example B: Barangui Stem Extract

A barley stem extract was prepared by extracting under the same conditions as in Manufacturing Example A, except that the hollow stem of barley ( Digitaria ciliaris ) was used instead of the leaves of the barley plant.

Manufacturing Example C: Extract of the leaves of the knotweed

Polygonum aviculare leaf extract was prepared by extracting under the same conditions as in Manufacturing Example A, except that Polygonum aviculare leaves were used instead of Polygonum aviculare leaves.

Manufacturing Example D: Blackcurrant Leaf Extract

Solanum nigrum leaf extract was prepared by extracting under the same conditions as in Manufacturing Example A, except that Solanum nigrum leaves were used instead of Solanum nigrum leaves.

Manufacturing Example 1: Lactobacillus plantarum culture using hot spring water

Lactobacillus plantarum strain (Lactobacillus plantarum 10 ⁸ CFU/㎖) was inoculated into a mixed medium consisting of 70㎖ of MRS Broth liquid medium and 30㎖ of hot spring water from the Dogo region, and 1㎖ of Lactobacillus plantarum strain was fermented at 37℃ for 30 hours. After the culture was homogenized, the cells were disrupted to obtain a Lactobacillus plantarum culture.

Manufacturing Example 2: Bifidobacterium longum culture using hot spring water

A Bifidobacterium longum culture was obtained by culturing under the same conditions as in Manufacturing Example 1, except that the Bifidobacterium longum strain was used instead of Lactobacillus plantarum.

Comparative manufacturing example 1: Culture of Lactobacillus plantarum cells removed using hot spring water

A Lactobacillus plantarum culture was prepared in the same manner as in Manufacturing Example 1, except that instead of treating the culture with a homogenizer after fermentation to disrupt the cells, the culture was centrifuged to remove the cells and a filtrate was obtained.

Comparative manufacturing example 2: Culture of Bifidobacterium longum cells removed using hot spring water

A Bifidobacterium longum culture was prepared in the same manner as in Manufacturing Example 2, except that instead of treating the culture with a homogenizer after fermentation to disrupt the cells, the culture was centrifuged to remove the cells and a filtrate was obtained.

Comparative Manufacturing Example 3: Lactobacillus plantarum culture without hot spring water

A Lactobacillus plantarum culture was prepared in the same manner as in Manufacturing Example 2, except that a medium that did not contain hot spring water was used.

Comparative Manufacturing Example 4: Bifidobacterium longum culture without using hot spring water

A Bifidobacterium longum culture was prepared in the same manner as in Manufacturing Example 1, except that a medium that did not contain hot spring water was used.

Example 1: Probiotic complex culture + plant complex extract

A cosmetic composition was prepared by mixing the Lactobacillus plantarum culture of Manufacturing Example 1, the Bifidobacterium longum culture of Manufacturing Example 2, and a plant complex extract in a volume ratio of 1:1:3. Here, the plant complex extract is a mixture of the dandelion leaf extract of Manufacturing Example A, the barley stem extract of Manufacturing Example B, and the knotweed leaf extract of Manufacturing Example C in a volume ratio of 1:1:1.

Example 2: Probiotic complex culture + plant complex extract

A cosmetic composition was prepared by mixing the Lactobacillus plantarum culture of Manufacturing Example 1, the Bifidobacterium longum culture of Manufacturing Example 2, and a plant complex extract in a volume ratio of 1:1:3. Here, the plant complex extract is a mixture of the dandelion leaf extract of Manufacturing Example A, the barley stem extract of Manufacturing Example B, the knotweed leaf extract of Manufacturing Example C, and the black sorrel leaf extract of Manufacturing Example D in a volume ratio of 1:1:1:1.

Comparative Example 1: Probiotics single culture + plant complex extract

A cosmetic composition was prepared under the same conditions as Example 2, except that the Lactobacillus plantarum culture of Manufacturing Example 1 was not used.

Comparative Example 2: Probiotics single culture + plant complex extract

A cosmetic composition was prepared under the same conditions as Example 2, except that the Bifidobacterium longum culture of Manufacturing Example 2 was not used.

Comparative Example 3: Probiotics complex culture with removed cells + plant complex extract

A cosmetic composition was manufactured under the same conditions as in Example 2, except that the Lactobacillus plantarum culture of Comparative Manufacturing Example 1 from which cells were removed and the Bifidobacterium longum culture of Comparative Manufacturing Example 2 from which cells were removed were used instead of the Lactobacillus plantarum culture of Manufacturing Example 1 and the Bifidobacterium longum culture of Manufacturing Example 2.

Comparative Example 4: Probiotics complex culture + plant complex extract without using hot spring water

A cosmetic composition was manufactured under the same conditions as in Example 2, except that the Lactobacillus plantarum culture of Manufacturing Example 1 and the Bifidobacterium longum culture of Manufacturing Example 2 were replaced with the Lactobacillus plantarum culture of Comparative Manufacturing Example 3 that did not use hot spring water and the Bifidobacterium longum culture of Comparative Manufacturing Example 4 that did not use hot spring water.

Comparative Example 5: Probiotic complex culture + single plant extract

A cosmetic composition was prepared under the same conditions as Example 2, except that only one extract of the leaves of the plant A was used instead of the four types of plant complex extracts.

Comparative Example 6: Probiotic complex culture + single plant extract

A cosmetic composition was prepared under the same conditions as Example 2, except that only one type of barley stem extract of Preparation Example B was used instead of the four types of plant complex extracts.

Comparative Example 7: Probiotic complex culture + single plant extract

A cosmetic composition was prepared under the same conditions as Example 2, except that only one type of knotweed leaf extract of Preparation Example C was used instead of the four types of plant complex extracts.

Comparative Example 8: Probiotic complex culture + single plant extract

A cosmetic composition was prepared under the same conditions as Example 2, except that only one extract of the leaves of the Chinese chive plant of Manufacturing Example D was used instead of the four types of plant complex extracts.

Comparative Example 9: Probiotic complex culture

A cosmetic composition was prepared under the same conditions as in Example 2, except that no plant complex extract was used.

Comparative Example 10: Plant Complex Extract

A cosmetic composition was manufactured under the same conditions as Example 2, except that the Lactobacillus plantarum culture of Manufacturing Example 1 and the Bifidobacterium longum culture of Manufacturing Example 2 were not used.

The manufacturing conditions of the cosmetic compositions of Examples 1 and 2 and Comparative Examples 1 to 10 are summarized in Table 1 below.

division Whether the badge contains hot spring water Cultured strain Plant extracts Whether the culture contains mycelia Lactobacillus plantarum Bifidobacterium longum Gaebireum leaves Barangi stem knotweed leaves Blackthorn leaves Example 1 O O O O O O X O Example 2 O O O O O O O O Comparative Example 1 O X O O O O O O Comparative Example 2 O O X O O O O O Comparative Example 3 O O O O O O O X Comparative Example 4 X O O O O O O O Comparative Example 5 O O O O X X X O Comparative Example 6 O O O X O X X O Comparative Example 7 O O O X X O X O Comparative Example 8 O O O X X X O O Comparative Example 9 O O O X X X X O Comparative Example 10 - X X O O O O O

[Experimental example]

All statistical analyses were performed using SPSS version 18.0 (IBM, Chicago, IL, USA). One-way ANOVA with Duncan's post-hoc test was used to determine the difference in mean values between experimental groups (p <0.05). Each experimental data was expressed as mean ± standard deviation (SD).

Experimental Example 1: Cytotoxicity Analysis

Cytotoxicity was measured using the MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reduction method. Cultured HaCaT cells were well mixed in DMEM medium, adjusted to 1x10 ⁵ cells/mL, and 1 mL of the prepared cells were added to a 24-well culture plate and cultured for 3 days. When the cells grew about 80%, the samples of Examples 1 and 2 and Comparative Example 3 were treated at various concentrations (0, 25, 50, 100, 300, 400, 500, 1000 ㎍/㎖) in a medium not containing fetal bovine serum and antibiotics, and then cultured for 24 hours more and the viability of the cells after culture was measured using the MTT reduction method.

After adding about 1/10 of the growth medium volume of MTT solution (5 mg/㎖) to each well, the wells were incubated at 37°C for another 4 hours. Afterwards, the medium was carefully removed so as not to lose the formazan produced by reducing MTT, and the remaining medium was left at room temperature for 30 minutes to completely remove the medium. The sample was dissolved using DMSO and the absorbance was measured at 570 nm. The cell viability was calculated according to Equation 1 below, and the results are shown in Table 2.

[Formula 1]

Cell viability (%) = [absorbance of sample treatment group/absorbance of control group] x 100

division Concentration (㎍/㎖) 10 100 Unprocessed 99±0.8 Example 1 97.0±1.1 93.2±1.2 Example 2 96.9±0.8 93.6±0.8 Comparative Example 3 97.4±0.5 93.3±1.3

Accordingly, it was confirmed that not only the samples of Examples 1 and 2 of the present invention but also the sample of Comparative Example 3 can be safely used on human skin. Since the samples of Comparative Examples 1, 2 and 4 to 10 are also included in the components of Examples 1, 2 or Comparative Example 3, separate cytotoxicity was not performed.

Experimental Example 2: Analysis of MMP-1 synthesis inhibition effect

CCD-986sk was cultured in a 35 mm dish at a concentration of ^1.5X105 cells/㎖ until it reached about 80% confluency. Then, the original medium was removed, washed with PBS to remove the serum component in the medium, and UVA (6.3 J/㎠) was irradiated for 24 hours using a UV light source equipped with a filter (Coralife, 35W, UV). After UVA irradiation, the samples of the examples or comparative examples of the present invention were respectively administered at concentrations of 10 and 100 ㎍/㎖ to DMEM medium to which FBS was not added, and then cultured for 24 hours. The cultured medium was dispensed into a 96-well plate and reacted overnight at 4℃. The measurement of MMP-1 expression level induced by UVA irradiation was performed using the method used by Dunsmore et al.

First, the medium was washed three times with TBS (phosphate buffered saline + 0.05% Tween 20), and then blocked with 3% PBS at 37°C for 1 hour. Then, a solution of primary antibody (monoclonal anti-MMP-1 antibody) diluted in a volume ratio of 1:3000 with blocking buffer was added, and the mixture was reacted at 37°C for 90 minutes. Then, the mixture was washed with PBS, and a solution of secondary antibody (alkaline phosphatase conjugated goat anti-mouse IgG) diluted in a volume ratio of 1:3,000 with blocking buffer was added, and the mixture was reacted at 37°C for 90 minutes. Then, the mixture was washed with PBS, and an alkaline phosphatase substrate solution (1 mg/mL, p-mitrophenylphosphate in diethanolamine buffer) was added, and the mixture was reacted at room temperature for 30 minutes, and then the reaction was stopped with 3N NaOH. Next, the absorbance was measured at 405 nm using a microplate reader, and the results are shown in Table 3 below.

division Concentration (㎍/㎖) 10 100 Unprocessed 8854±28 Example 1 6120±48 5025±80 Example 2 5302±52 4120±39 Comparative Example 1 7140±90 6806±72 Comparative Example 2 7590±101 7278±45 Comparative Example 3 7904±45 7095±87 Comparative Example 4 6808±51 5609±23 Comparative Example 5 7840±110 6740±76 Comparative Example 6 7857±75 6003±57 Comparative Example 7 7724±40 6927±49 Comparative Example 8 7926±68 7090±87 Comparative Example 9 7663±58 7123±98 Comparative Example 10 7520±61 7090±113

Accordingly, the sample of Example 2 containing a complex culture of two types of probiotics and a complex extract of four types of plants showed the highest inhibition effect on the synthesis of MMP-1 protein compared to the other experimental groups, and the sample of Example 1 containing a complex culture of two types of probiotics and a complex extract of three types of plants also showed a significantly higher inhibition effect on the synthesis of MMP-1 protein compared to the comparative examples. In contrast, the culture of Comparative Example 3, in which the bacterial cells were removed from the culture, showed a lower inhibition effect on the synthesis of MMP-1 protein compared to Examples 1 and 2, which included the bacterial cells, and Comparative Examples 1 and 2, which used only one type of fermentation strain, also showed a relatively lower inhibition effect on the synthesis of MMP-1 protein, a collagen-decomposing enzyme. Therefore, the cosmetic compositions of Examples 1 and 2 are expected to be more effective in inhibiting the formation of wrinkles than the cosmetic compositions of the comparative examples.

Experimental Example 3: Analysis of collagen synthesis promotion effect

In order to confirm the skin wrinkle improvement efficacy of the samples of Examples 1 and 2 and Comparative Examples 1 to 10, the amount of collagen, a protein that maintains skin elasticity, produced was measured. Fibroblasts were seeded at a density of ^1X104 cells/well in a 48-well plate and cultured for 24 hours in DMEM medium containing 10% FBS. Then, the samples of the examples or comparative examples were added at 50 and 100 ㎍/㎖ and cultured for an additional 24 hours in serum-free medium. After culture, the supernatant from each well was collected, and the amount of procollagen type I C-peptide (PICP) was measured using a procollagen type I C-peptide EIA kit (MK101, Takara, Japan) and converted to ng/㎖. The amount of collagen synthesized thereby was measured, and the results are shown in Table 4.

division Concentration (㎍/㎖) 50 100 Unprocessed 40.1±0.8 Example 1 75.8±0.7 85.5±1.2 Example 2 82.2±1.2 92.4±0.8 Comparative Example 1 58.8±1.2 63.9±1.1 Comparative Example 2 57.7±0.7 65.2±0.3 Comparative Example 3 57.5±1.1 66.8±0.8 Comparative Example 4 80.5±0.7 88.2±0.6 Comparative Example 5 49.5±1.3 64.7±0.4 Comparative Example 6 48.6±0.9 58.8±0.6 Comparative Example 7 51.8±1.0 63.5±1.6 Comparative Example 8 53.5±0.7 65.8±2.1 Comparative Example 9 58.9±1.5 67.0±0.7 Comparative Example 10 47.3±1.2 59.0±0.6

Accordingly, the sample manufactured according to Example 1 or 2 of the present invention showed the most excellent effect of promoting collagen production compared to the untreated group and the comparative examples. In addition, in the case of Comparative Example 4 including a culture that did not use hot spring water, a collagen production promoting effect at a level similar to that of Example 1 or 2 was shown. In contrast, in Comparative Examples 1 and 2 having a single culture of probiotics, Comparative Example 3 including a culture from which the bacterial cells were removed, Comparative Examples 5 to 8 including a single plant extract, Comparative Example 9 including only a probiotic complex culture, and Comparative Example 10 including only a plant complex extract, the collagen production promoting effect was shown to be relatively low.

Experimental Example 4: Tyrosinase Inhibition Activity Analysis

For the samples of Examples 1 and 2 and Comparative Examples 1 to 10, tyrosinase inhibition ability was measured by the method of Yagi et al. (1986). Melanin exists in the basal layer of the epidermis, and melanin synthesis is regulated by tyrosinase in the intracellular melanosomes of melanocytes, and therefore, inhibition of tyrosinase activity can reduce melanin synthesis and pigmentation. 0.2 ml of a substrate solution containing 10 mM L-DOPA dissolved in 0.5 ml of 1.15 M sodium phosphate buffer, pH 6.8, and 0.1 ml of a sample solution were mixed in a test tube, to which 0.2 ml of 110 Unit/ml mushroom tyrosinase was added. After reacting at 25°C for 2 minutes, the absorbance of DOPA chrome generated in the reaction solution was measured at 490 nm using a microplate reader. Tyrosinase inhibition activity was expressed as the decrease in absorbance between the added and unadded groups of the sample solution, and the results are shown in Table 5 below.

division Treatment concentration (% (w/v)) 50 100 Example 1 6.5±1.4 10.4±0.5 Example 2 6.9±0.8 11.5±1.0 Comparative Example 1 4.5±0.5 6.5±0.8 Comparative Example 2 3.5±1.2 5.5±0.4 Comparative Example 3 3.8±0.4 6.8±0.9 Comparative Example 4 6.3±0.5 9.7±0.7 Comparative Example 5 3.1±0.6 6.8±0.7 Comparative Example 6 4.2±0.3 5.9±0.3 Comparative Example 7 3.3±0.2 6.7±0.8 Comparative Example 8 2.9±1.3 5.8±1.1 Comparative Example 9 3.9±0.9 5.9±0.6 Comparative Example 10 3.0±0.9 5.6±0.5

Accordingly, the sample of Example 1 or 2 of the present invention was shown to have the highest tyrosinase inhibitory activity, and in the case of Comparative Example 4 including a culture that did not use hot spring water, tyrosinase inhibitory activity at a similar level to Example 1 or 2 was shown. In contrast, Comparative Examples 1 and 2 having single cultures of probiotics, Comparative Example 3 including a culture from which the bacterial cells were removed, Comparative Examples 5 to 8 including single plant extracts, Comparative Example 9 including only a probiotic complex culture, and Comparative Example 10 including only a plant complex extract showed relatively low tyrosinase inhibitory activity.

Experimental Example 5: Analysis of Melanin Production Inhibition Ability

B16F1 cells, which are mouse-derived melanocytes, produce melanin, and melanin is synthesized in melanosomes, organelles within melanocytes located in the basal layer of the epidermis. The amount produced can be compared by measuring the absorbance in the visible light range. After B16F1 cell inoculation, samples were processed for 3 days, the medium was removed, the cells were washed with PBS, 1 ㎖ of 1 N NaOH was added to each well, and the mixture was stirred to dissolve the cell membrane and release the melanin component. The amount of melanin produced was measured by measuring the absorbance in the blue visible light range with the shortest wavelength of 400 nm in the visible light range.

The above melanin amount was measured by a method expressing it as absorbance per unit cell number (10 ⁶ cells), and the relative melanin production compared to the control group (untreated group) was calculated as the inhibition rate (%), and the results are summarized in Table 6.

Classification (unit: %) Concentration (㎍/㎖) 50 100 Unprocessed 41.2±0.5 Example 1 68.7±0.4 70.6±0.8 Example 2 66.2±0.7 74.5±1.1 Comparative Example 1 44.5±0.4 47.7±0.2 Comparative Example 2 53.0±1.2 59.2±0.8 Comparative Example 3 43.7±0.9 52.5±1.0 Comparative Example 4 60.4±0.5 72.2±0.5 Comparative Example 5 36.2±0.9 45.7±0.8 Comparative Example 6 42.0±0.2 54.7±0.5 Comparative Example 7 38.5±0.5 53.6±0.9 Comparative Example 8 42.1±1.0 49.3±0.4 Comparative Example 9 48.0±1.1 48.7±1.2 Comparative Example 10 50.1±0.5 56.1±0.4

Accordingly, the samples of Examples 1 and 2 showed the best melanin production inhibition ability, and in the case of Comparative Example 4 including a culture that did not use hot spring water, melanin production inhibition ability at a level similar to that of Example 1 or 2 was shown. In contrast, Comparative Examples 1 and 2 having single cultures of probiotics, Comparative Example 3 including a culture from which the fungi were removed, Comparative Examples 5 to 8 including single plant extracts, Comparative Example 9 including only a probiotic complex culture, and Comparative Example 10 including only a plant complex extract showed relatively low melanin production inhibition ability.

A formulation example of a cosmetic composition suitable for applying the present invention is presented.

Preparation Example 1: Toner

An example of a formulation of a toner using the cosmetic composition of Example 2 is as shown in Table 7 below.

ingredient Content (weight%) Composition of Example 2 5.0 glycerin 6.0 1,3-Butylene Glycol 3.0 PEG1500 1.0 Allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaluronate 5.0 Ethanol 10.0 Polysorbate 20 0.2 Preservatives, fragrances, colorings a very small amount Distilled water Remaining amount total 100

Example 2: Lotion

An example of a lotion formulation using the cosmetic composition of Example 2 is as shown in Table 8 below.

ingredient Content (weight%) Composition of Example 2 3.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocopheryl acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitan sesquioleate 1.0 Carboxyvinyl polymer 1.0 Preservatives, fragrances, colorings a very small amount Distilled water Remaining amount total 100

Example 3: BB Cream

An example of a BB cream formulation using the cosmetic composition of Example 2 is as shown in Table 9 below.

ingredient Content (weight%) Composition of Example 2 1.0 Monostearate glycerin 1.5 Cetearyl alcohol 1.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Isostearyl isostearate 5.0 Squalane 5.0 Mineral oil 35.0 Dimethicone 0.5 Hydroxyethylcellulose 0.12 glycerin 6.0 Triethanolamine 0.7 Preservatives, fragrances, colorings a very small amount Distilled water Remaining amount total 100

Example 4: Essence

An example of an essence formulation using the cosmetic composition of Example 2 is as shown in Table 10 below.

ingredient Content (weight%) Composition of Example 2 1.5 glycerin 10.0 Betaine 5.0 PEG 1500 2.0 Allantoin 0.1 DL-Panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethylcellulose 0.1 Sodium hyaluronate 8.0 Carboxyvinyl polymer 0.2 Triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeceth-16 0.4 Ethanol 6.0 Preservatives, fragrances, colorings a very small amount Distilled water Remaining amount total 100

Preparation Example 5: Emulsion for mask pack

An example of the formulation of a lotion for a mask pack using the cosmetic composition of Example 2 is as shown in Table 11 below.

ingredient Content (weight%) Composition of Example 2 1.0 polyvinyl alcohol 15.0 Cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 Cyclodextrin 0.15 DL-Panthenol 0.4 Allantoin 0.1 Monoammonium glycyrrhizinate 0.3 Nicotinamide 0.5 Ethanol 6.0 PEG 40 hydrogenated castor oil 0.3 Preservatives, fragrances, colorings a very small amount Distilled water Remaining amount total 100

Above, the embodiments of the present invention have been described, but those skilled in the art will be able to modify and change the present invention in various ways by adding, changing, deleting or adding components, etc., within the scope that does not depart from the spirit of the present invention described in the claims, and this will also be considered to be included within the scope of the rights of the present invention.

Claims (9)

A culture comprising a strain of the genus Lactobacillus cultured in a medium containing hot spring water;
A culture comprising a strain of the genus Bifidobacterium cultured in a medium containing hot spring water; and
Contains plant complex extracts including Amaranthus lividus leaf extract, Digitaria ciliaris stem extract and Polygonum aviculare L. extract as active ingredients;
The above Lactobacillus strain is Lactobacillus plantarum.
A cosmetic composition, characterized in that the Bifidobacterium genus strain is Bifidobacterium longum . In the first paragraph,
A cosmetic composition characterized in that the above plant complex extract additionally contains Solanum nigrum leaf extract. In the second paragraph,
A cosmetic composition characterized in that the above plant complex extract is extracted by water, an alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. In the first paragraph,
A cosmetic composition, characterized in that the medium containing the hot spring water contains 10 to 40% (v/v) of hot spring water. In the first paragraph,
A cosmetic composition, characterized in that the plant complex extract is contained in an amount of 50 to 70% (v/v) in the cosmetic composition. In paragraph 5,
A cosmetic composition, characterized in that the culture containing the Lactobacillus genus strain and the culture containing the Bifidobacterium genus strain are included in a volume ratio of 10:5 to 10:15 in the cosmetic composition. delete delete In the first paragraph,
The above cosmetic composition is characterized in that it is for skin whitening, wrinkle suppression or wrinkle improvement.