KR102681111B1 - Cosmetic composition containing fragrant natural extract complex, and Manufacturing method thereof - Google Patents
Cosmetic composition containing fragrant natural extract complex, and Manufacturing method thereof Download PDFInfo
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- KR102681111B1 KR102681111B1 KR1020230135022A KR20230135022A KR102681111B1 KR 102681111 B1 KR102681111 B1 KR 102681111B1 KR 1020230135022 A KR1020230135022 A KR 1020230135022A KR 20230135022 A KR20230135022 A KR 20230135022A KR 102681111 B1 KR102681111 B1 KR 102681111B1
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
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- A61Q13/00—Formulations or additives for perfume preparations
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
Landscapes
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- Animal Behavior & Ethology (AREA)
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Abstract
본 발명은, 발향 가능한 천연 추출 복합물을 유효성분으로 함유하는 화장료 조성물 및 그 제조방법에 관한 것으로, 발향 천연 식물의 하이드로졸(hydrosol)과 추출물(extract)을 유효성분으로 함유하는 천연 추출 복합물을 함유함으로써 발향이 가능하고 미백, 주름개선, UV에 의한 세포 손상 보호와 같은 항노화 효과를 나타내는 효과가 있다.The present invention relates to a cosmetic composition containing a fragrant natural extract complex as an active ingredient and a method for producing the same, which contains a natural extract complex containing a hydrosol and extract of a fragrant natural plant as an active ingredient. By doing so, it can be scented and has anti-aging effects such as whitening, wrinkle improvement, and protection from cell damage caused by UV.
Description
본 발명은 발향 가능한 천연 추출 복합물을 유효성분으로 함유하는 화장료 조성물 및 그 제조방법에 관한 것으로, 더욱 상세하게는 발향 천연 식물의 하이드로졸(hydrosol)과 추출물(extract)을 유효성분으로 함유하는 천연 추출 복합물을 함유함으로써 발향이 가능하고 미백, 주름개선, UV에 의한 세포 손상 보호와 같은 항노화 효과를 나타내는 화장료 조성물 및 그 제조방법에 관한 것이다. The present invention relates to a cosmetic composition containing a fragrant natural extract complex as an active ingredient and a method for producing the same. More specifically, it relates to a natural extract containing hydrosol and extract of a fragrant natural plant as an active ingredient. It relates to a cosmetic composition that contains a complex, allows for scenting, and exhibits anti-aging effects such as whitening, wrinkle improvement, and protection from cell damage caused by UV, and a method for producing the same.
최근에 화장품에 이용되는 향료들은 착향제 내에 포함되어 있는 25가지 알러젠 물질들을 기재하도록 화장품법 시행규칙이 개정(개정 2019.12.16, 시행 2020.1.1.)되어 관심이 급증하고 있다.Recently, interest in fragrances used in cosmetics is rapidly increasing as the Enforcement Rules of the Cosmetics Act were revised to list 25 allergens contained in flavoring agents (revised on December 16, 2019, effective January 1, 2020).
이 착향제에는 단순히 합성 착향제 뿐 아니라 천연 에센셜 오일 또한 착향제로 분류되고 있어 단순히 천연 착향제와 합성 착향제로 구분하여 25가지 알러젠 물질 기재를 회피하는 것은 불가능하다. 따라서 착향제로 분류되지 않는 천연 추출 복합물들을 이용하여 발향이 가능하도록 화장품에 이용하는 것은 착향제를 이용하지 않고 화장품에 향을 내는 좋은 방법이 될 수 있다.These flavoring agents include not only synthetic flavoring agents but also natural essential oils. Therefore, it is impossible to avoid listing 25 allergens by simply classifying them as natural flavoring agents and synthetic flavoring agents. Therefore, using natural extract compounds that are not classified as flavoring agents in cosmetics to enable scenting can be a good way to add fragrance to cosmetics without using flavoring agents.
한편 우리나라는 2018년 기준 도시계획현황 통계조사를 기준으로 총인구 5,182만명중 4,759만명이 도시지역에 거주하는 것으로 나타나 91.8%의 높은 비율로 대다수의 인구가 도시에 거주하는 것으로 분석되었다. 이에 따라 도시에 거주하면서 나타나는 미세먼지와 환경오염, 자외선과 같은 외부 환경에 의한 스트레스와 담배, 알코올 섭취, 수면 부족과 같은 개인의 라이프스타일(life style), 공부나 직장생활에서 오는 스트레스 등에 의해 사람의 피부는 영향을 받는다. Meanwhile, in Korea, based on the 2018 urban planning status statistical survey, 47.59 million out of the total population of 51.82 million were found to live in urban areas, indicating that the majority of the population lives in cities, with a high rate of 91.8%. Accordingly, people are affected by stress caused by the external environment such as fine dust, environmental pollution, and ultraviolet rays that appear while living in the city, as well as personal lifestyle such as smoking, alcohol consumption, and lack of sleep, and stress from studying or work life. The skin of is affected.
이러한 외부적인 요인과 개인의 유전적인 소인이 더해져 개인의 최종적인 피부의 노화정도가 결정되게 된다. 특히나 외부 환경에 의한 스트레스 중 UVB(280~320nm)를 포함한 자외선에서의 피부 노출은 피부에서 광노화 뿐만 아니라 피부 암까지도 유발하는 자극원으로 알려져있다. 자세하게는 일광화상, 과색소 침착, DNA 손상, 활성산소종 형성, 피부암을 발생시키며, 피부에는 엘라스틴, 피브릴린, 콜라겐을 분해하여 탄력 감소 및 주름형성을 촉진하는 피부 노화를 유발한다고 알려져 있다.These external factors and the individual's genetic predisposition are added to determine the individual's final degree of skin aging. In particular, skin exposure to ultraviolet rays, including UVB (280-320 nm), among external environmental stressors, is known to be a irritant that causes not only photoaging of the skin but also skin cancer. Specifically, it is known to cause sunburn, hyperpigmentation, DNA damage, formation of reactive oxygen species, and skin cancer, and to cause skin aging by decomposing elastin, fibrillin, and collagen in the skin, reducing elasticity and promoting wrinkle formation.
또한, 이와 같은 외부 환경 스트레스 요인들과 개인의 내재적인 요인들은 피부의 노화뿐 아니라 피부 흑화와도 연관이 있는 것으로 보고되어 있다. 피부의 흑화란 멜라노사이트(melanocyte)내에 타이로시나제(tyrosinase)가 타이로신을 산화시켜 결론적으로 멜라노좀(melanosome)을 형성하는 것으로 이렇게 형성된 멜라노좀은 각질형성세포(keratinocyte)로 전달되어 자외선으로부터 피부를 보호하며 활성산소(active oxygen species)(ROS)를 제거하는 등의 역할을 담당하게 된다. 그러나 과도하게 생성된 멜라노좀은 피부 표면에 침착되어 기미나 주근깨 등을 나타내게 되어 지양되고 있다. 특히나 아시아 여성들은 잡티가 없는 하얀 피부를 선호하는 기호가 있어 미백 화장품을 사용하는 비율이 높게 나타난다.In addition, it has been reported that such external environmental stress factors and internal personal factors are related to not only skin aging but also skin darkening. Darkening of the skin occurs when tyrosinase oxidizes tyrosine within melanocytes, ultimately forming melanosomes. The melanosomes thus formed are transferred to keratinocytes and protect the skin from ultraviolet rays. It plays a role in protecting and removing reactive oxygen species (ROS). However, excessively produced melanosomes are deposited on the skin surface and cause spots or freckles, so they are avoided. In particular, Asian women prefer white skin without blemishes, so the use of whitening cosmetics is high.
따라서 착향제로 분류되지 않는 천연 식물의 추출물 및 하이드로졸들을 이용하여 화장품 내에 발향이 가능하도록 만든 천연 추출 복합물에 대한 수요가 발생할 것으로 예상되며 이렇게 조성된 천연 추출 복합물이 피부에 미백과 주름개선, 세포보호와 같은 생리적인 활성을 나타내는 것을 확인하여, 본 발명을 완성하였다.Therefore, it is expected that there will be a demand for natural extract complexes made to allow fragrance in cosmetics using extracts and hydrosols from natural plants that are not classified as fragrances. Natural extract complexes made in this way are expected to whiten the skin, improve wrinkles, and improve cell growth. By confirming that it exhibits physiological activity such as protection, the present invention was completed.
본 발명의 목적은 발향 천연 식물의 하이드로졸(hydrosol)과 추출물(extract)을 유효성분으로 포함하는 천연 추출 복합물을 제공하고 이러한 천연 추출 복합물을 화장료 조성물에 유효성분으로 함유시켜 발향이 가능하고 미백 및 주름 개선, UV에 의한 세포 손상 회복과 같은 항노화 효과를 갖는 화장료 조성물을 제공하는 것이다. The purpose of the present invention is to provide a natural extraction complex containing hydrosol and extract of scented natural plants as active ingredients, and to include this natural extract complex as an active ingredient in a cosmetic composition to enable scenting and whitening and The aim is to provide a cosmetic composition that has anti-aging effects such as improving wrinkles and recovering cell damage caused by UV.
상기 목적을 달성하기 위하여, In order to achieve the above purpose,
본 발명은 발향 천연 식물의 하이드로졸(hydrosol)과 추출물(extract)을 유효성분으로 포함하는 천연 추출 복합물을 제조하는 것을 특징으로 한다. The present invention is characterized by producing a natural extract complex containing hydrosol and extract of fragrant natural plants as active ingredients.
본 발명의 용어, '하이드로졸(Hydrosol(Floral Water))'은 발향 천연 식물 등에서 증류법을 사용하여 추출된 에센셜 오일의 수용액 혹은 클로이드 부유물을 일컬으며, 일반적으로 아로마 식물들을 스팀 증류하여 얻어진다. 하이드로졸은 에센셜 오일을 추출하는 과정에서 동시에 얻어지며, 에센셜 오일은 상층 부분에 떠있게 되고 이를 제거하여 물과 같은 증류액, 즉 하이드로졸을 수득한다. The term 'Hydrosol (Floral Water)' in the present invention refers to an aqueous solution or cloid suspension of essential oil extracted from aromatic natural plants using a distillation method, and is generally obtained by steam distillation of aromatic plants. Hydrosol is simultaneously obtained during the process of extracting essential oil. The essential oil floats in the upper layer and is removed to obtain a water-like distillate, that is, hydrosol.
본 발명에서 하이드로졸(Hydrosol) 제조방법은 발향 천연 식물인 아로마 식물의 꽃잎, 잎사귀, 줄기, 뿌리 등을 수득하여, 일정한 크기로 세절 및 세척하여 건조한다. 건조한 아로마 식물에 증류수를 넣어 수증기 증류 추출장치에서 100 oC 이상의 고온 추출을 하고 상층부의 오일이 제거된 증류액을 콘덴서를 통해 응축하여 고유의 특이 향취를 포함하는 하이드로졸을 수득한다. 하이드로졸은 보관을 위해 프로판다이올, 부틸렌글라이콜, 펜틸렌글라이콜, 1,2-헥산다이올, 에틸헥실글리세린으로 구성된 군으로부터 선택된 하나 이상의 용제를 투입하여 교반하고 여과하여 보관한다.In the present invention, the hydrosol production method involves obtaining the petals, leaves, stems, roots, etc. of aroma plants, which are natural fragrant plants, cutting them to a certain size, washing them, and drying them. Distilled water is added to dried aroma plants, extraction is performed at a high temperature of over 100 o C in a steam distillation extraction device, and the distillate from which the oil in the upper layer has been removed is condensed through a condenser to obtain a hydrosol containing its own unique aroma. For storage, the hydrosol is stored by adding at least one solvent selected from the group consisting of propanediol, butylene glycol, pentylene glycol, 1,2-hexanediol, and ethylhexylglycerin, stirring, filtering, and storing.
또한, '추출물(extract)'은 발향 천연 식물 등에서 증류법을 사용하여 추출된 수상인 하이드로졸과는 다르게 침지법을 사용하여 추출된 수상을 칭한다. In addition, 'extract' refers to an aqueous phase extracted using a immersion method, unlike hydrosol, which is an aqueous phase extracted from fragrant natural plants, etc. using a distillation method.
본 발명에서 추출물(Extract) 제조방법은 발향 천연 식물인 아로마 식물의 꽃잎, 잎사귀, 줄기, 뿌리 등을 수득하여, 일정한 크기로 세절 및 세척하여 건조한다. 건조한 아로마 식물을 정제수, 에탄올, 부틸렌글라이콜 및 프로판다이올, 펜틸렌글라이콜로 구성된 군으로부터 선택된 하나 이상의 용매에 투입하여 40 oC - 100 oC 사이의 온도에서 추출하여 고유의 특이 향취를 포함하는 추출물을 수득한다. 수득한 추출물은 저온에서 냉침하여 여과한다.In the present invention, the extract manufacturing method obtains the petals, leaves, stems, roots, etc. of aroma plants, which are natural plants with a scent, and cuts them to a certain size, washes them, and dries them. Dried aroma plants are added to one or more solvents selected from the group consisting of purified water, ethanol, butylene glycol, propanediol, and pentylene glycol, and extracted at a temperature between 40 o C and 100 o C to contain their unique, specific scent. An extract is obtained. The obtained extract is cold-soaked at low temperature and filtered.
또한, 본 발명의 용어, '천연 추출 복합물'이란 아로마 식물과 같은 발향 천연 식물 등에서 증류법, 침지법등 다양한 수단으로 발향이 가능하도록 추출된 하이드로졸(hydrosol)과 추출물(extract)을 포함하는 천연물들의 복합 조성물을 의미한다In addition, the term 'natural extraction complex' in the present invention refers to natural products including hydrosols and extracts extracted from fragrant natural plants such as aroma plants by various means such as distillation and immersion. refers to a composite composition
또한, 본 발명의 용어, '항노화'는 피부 상태의 개선이나 완화와 같은 의미를 뜻하며, 구체적으로 피부의 주름개선, 미백, 보습, 탄력으로 구성된 군으로부터 선택된 하나 이상의 증상 개선을 의미할 수 있으나 이에 제한되는 것은 아니다.In addition, the term 'anti-aging' of the present invention means improvement or relief of skin condition, and may specifically mean improvement of one or more symptoms selected from the group consisting of wrinkle improvement, whitening, moisturizing, and elasticity of the skin. It is not limited to this.
여기서, 상기 하이드로졸은, 발향 천연 식물인 라임수, 비터오렌지꽃수, 오렌지꽃수, 오렌지수, 왕귤수, 레몬수, 만다린수, 편백수, 솔잣나무잎수, 두송열매수, 구주소나무잎수, 선백리향잎수, 동백꽃수, 귤꽃수, 위치하젤잎수, 위치하젤수, 위치하젤꽃수, 에버라스팅꽃수, 쟈스민꽃수, 센티드제라늄꽃수, 센티드제라늄수, 서양장미꽃수, 다마스크장미꽃수, 파인애플수, 코코넛수, 라즈베리수, 캐모마일꽃수, 애엽수, 카렌둘라꽃수, 수레국화꽃수, 병풀잎수, 캐모마일꽃수, 유칼립투스잎수, 라벤더꽃수, 라벤더수, 라반딘꽃수, 레몬버베나잎수, 레몬버베나수, 레몬밤꽃과 잎 및 줄기수, 로즈마리잎수, 세이지잎수, 세이지수, 클라리꽃과 잎 및 줄기수, 페퍼민트잎수, 티트리잎수, 회향수, 팔각회향수, 인삼수, 바닐라열매수, 피나무꽃수로 이루어진 군으로부터 선택된 어느 하나 이상을 이용하여 제조되는 것을 특징으로 한다.Here, the hydrosol is a fragrant natural plant lime water, bitter orange flower water, orange flower water, orange water, tangerine water, lemon water, mandarin water, cypress water, pine pine leaf water, dupine fruit water, Guju pine leaf water, and thyme. Leaf water, camellia flower water, tangerine flower water, witch hazel leaf water, witch hazel water, witch hazel flower water, everlasting flower water, jasmine flower water, scented geranium flower water, scented geranium water, western rose flower water, damask rose flower water, pineapple water, coconut Water, raspberry water, chamomile flower water, petal leaf water, calendula flower water, cornflower flower water, centella leaf water, chamomile flower water, eucalyptus leaf water, lavender flower water, lavender water, lavandin flower water, lemon verbena leaf water, lemon verbena water, lemon balm water. Selected from the group consisting of leaves and stem water, rosemary leaf water, sage leaf water, sage water, clary flower and leaf and stem water, peppermint leaf water, tea tree leaf water, fennel water, star aniseed fennel water, ginseng water, vanilla fruit water, and blood tree flower water. It is characterized in that it is manufactured using one or more of the following.
또한, 상기 추출물은, 발향 천연 식물인 다마스크장미꽃봉오리추출물, 다마스크장미추출물, 다마스크장미꽃추출물, 프렌치로즈꽃추출물, 장미꽃추출물, 사향장미잎추출물, 영실추출물, 장미추출물, 코코넛추출물, 산딸기추출물, 카제푸트열매추출물, 블랙베리추출물, 블랙베리잎추출물, 장딸기열매/잎추출물, 라즈베리추출물, 라즈베리잎추출물, 멍석딸기추출물, 블루베리추출물, 블루베리잎추출물, 하이부쉬블루베리추출물, 덩굴월귤열매추출물, 서양톱풀추출물, 서양톱풀꽃추출물, 아그리모니추출물, 아그리모니잎추출물, 캐모마일꽃추출물, 캐모마일꽃수, 쓴쑥추출물, 청호추출물, 유기노추출물, 사철쑥꽃추출물, 쑥잎추출물, 타라곤잎/줄기추출물, 타라곤뿌리추출물, 요모기잎추출물, 쑥캘러스추출물, 애엽추출물, 애엽수, 알파인웜우드추출물, 쑥추출물, 불가리스쑥과 슈크로오스발효추출물, 카렌둘라추출물, 카렌둘라꽃추출물, 수레국화꽃추출물, 병풀추출물, 병풀잎추출물, 병풀잎수, 캐모마일추출물, 캐모마일꽃추출물, 캐모마일꽃과 잎추출물, 캐모마일꽃과 잎 및 줄기추출물, 캐모마일잎추출물, 국화꽃추출물, 레몬그라스추출물, 레몬그라스추출물, 레몬그라스잎추출물, 레몬그라스잎과 줄기추출물, 유칼립투스잎추출물, 좁은잎페퍼민트검꽃과 잎 및 줄기추출물, 히솝추출물, 라벤더추출물, 라벤더꽃추출물, 라벤더꽃과 잎 및 줄기추출물, 라반딘꽃추출물, 더치라벤더꽃과 잎 및 줄기추출물, 프렌치라벤더추출물, 레몬버베나꽃추출물, 레몬버베나잎추출물, 레몬버베나잎수, 레몬밤추출물, 레몬밤꽃과 잎 및 줄기추출물, 레몬밤잎추출물, 로즈마리추출물, 로즈마리꽃추출물, 로즈마리잎추출물, 세이지추출물, 세이지잎추출물, 베르가못민트잎추출물, 박하추출물, 박하꽃과 잎 및 줄기추출물, 박하잎추출물, 콘민트추출물, 페퍼민트캘러스배양추출물, 페퍼민트추출물, 페퍼민트꽃과 잎 및 줄기추출물, 페퍼민트잎추출물, 페니로얄추출물, 애플민트잎추출물, 양박하꽃과 잎 및 줄기추출물, 스피어민트추출물, 스피어민트잎추출물, 서양고추나물추출물, 서양고추나물꽃과 잎 및 줄기추출물, 티트리추출물, 티트리꽃과 잎 및 줄기추출물, 티트리잎추출물, 회향추출물, 회향잎추출물, 회향뿌리추출물, 회향씨추출물, 스파이크드진저릴리추출물, 팔각회향추출물, 월계수잎추출물, 오레가노꽃추출물, 오레가노잎추출물, 오레가노잎과 잎 및 줄기추출물, 인삼열매추출물, 인삼캘러스배양추출물, 인삼형성층세포배양추출물, 인삼꽃추출물, 인삼잎과 줄기추출물, 산삼배양근추출물, 인삼씨추출물, 인삼싹추출물, 바닐라콩추출물, 바닐라꽃추출물, 바닐라열매추출물, 발삼캐나다추출물, 로즈우드추출물, 침향추출물, 서던우드추출물, 위치하젤껍질과 잎추출물, 위치하젤껍질과 잎 및 잔가지추출물, 위치하젤껍질과 잔가지추출물, 백단향목부추출물, 피나무추출물, 피나무꽃추출물, 피나무추출물, 헬리크리섬꽃추출물로 이루어진 군으로부터 선택된 어느 하나 이상을 이용하여 제조되는 것을 특징으로 한다.In addition, the extract includes damask rose bud extract, damask rose extract, damask rose flower extract, French rose flower extract, rose flower extract, musk rose leaf extract, Yeongsil extract, rose extract, and coconut extract, which are fragrant natural plants. , raspberry extract, caseput fruit extract, blackberry extract, blackberry leaf extract, long strawberry fruit/leaf extract, raspberry extract, raspberry leaf extract, strawberry extract, blueberry extract, blueberry leaf extract, highbush blueberry extract, Cranberry fruit extract, yarrow extract, yarrow flower extract, agrimony extract, agrimony leaf extract, chamomile flower extract, chamomile flower water, bitter mugwort extract, green tiger extract, organino extract, mugwort flower extract, mugwort leaf extract, tarragon Leaf/stem extract, tarragon root extract, Yomogi leaf extract, mugwort callus extract, leaf extract, leaf root, Alpine wormwood extract, mugwort extract, vulgaris mugwort and sucrose fermented extract, calendula extract, calendula flower extract, strawberry Chrysanthemum flower extract, centella asiatica extract, centella asiatica leaf extract, centella asiatica leaf water, chamomile extract, chamomile flower extract, chamomile flower and leaf extract, chamomile flower, leaf and stem extract, chamomile leaf extract, chrysanthemum flower extract, lemongrass extract, lemongrass Extract, lemongrass leaf extract, lemongrass leaf and stem extract, eucalyptus leaf extract, narrow-leaf peppermint gum flower, leaf and stem extract, hyssop extract, lavender extract, lavender flower extract, lavender flower, leaf and stem extract, lavandin flower extract, Dutch lavender flower, leaf and stem extract, French lavender extract, lemon verbena flower extract, lemon verbena leaf extract, lemon verbena leaf water, lemon balm extract, lemon balm flower, leaf and stem extract, lemon balm leaf extract, rosemary extract, rosemary flower extract, Rosemary leaf extract, sage extract, sage leaf extract, bergamot mint leaf extract, peppermint extract, peppermint flower, leaf and stem extract, peppermint leaf extract, corn mint extract, peppermint callus culture extract, peppermint extract, peppermint flower, leaf and stem extract , peppermint leaf extract, pennyroyal extract, apple mint leaf extract, spearmint flower, leaf and stem extract, spearmint extract, spearmint leaf extract, red pepper greens extract, red pepper greens flower, leaf and stem extract, tea tree extract, tea Tree flower, leaf and stem extract, tea tree leaf extract, fennel extract, fennel leaf extract, fennel root extract, fennel seed extract, spiked ginger lily extract, star aniseed fennel extract, bay leaf extract, oregano flower extract, oregano leaf extract, Oregano leaf, leaf and stem extract, ginseng fruit extract, ginseng callus culture extract, ginseng cambium cell culture extract, ginseng flower extract, ginseng leaf and stem extract, wild ginseng culture root extract, ginseng seed extract, ginseng bud extract, vanilla bean extract, vanilla Flower extract, vanilla fruit extract, Balsam Canada extract, rosewood extract, agarwood extract, southernwood extract, witch hazel bark and leaf extract, witch hazel bark, leaf and twig extract, witch hazel bark and twig extract, sandalwood xylem extract, blood tree. It is characterized in that it is manufactured using at least one selected from the group consisting of extract, Pinocchia flower extract, Pinocranial extract , and helichrysum flower extract.
상기 하이드로졸 및 추출물은, 천연 추출 복합물 총 중량대비 60중량% 내지 90중량%의 범위로 포함되는 것을 특징으로 한다. 함량이 60중량% 미만인 경우에는 뇌파의 안정화에 의한 스트레스 발생시 생성되는 베타파 감소를 통한 스트레스 완화의 심리적 안정의 효과를 기대할 수 없고, 90중량%를 초과할 경우 이취 및 안정도상 변취에 대한 문제점이 있다.The hydrosol and extract are characterized in that they are contained in the range of 60% by weight to 90% by weight based on the total weight of the natural extract complex. If the content is less than 60% by weight, the effect of psychological stability in relieving stress by reducing beta waves generated when stress occurs by stabilizing brain waves cannot be expected, and if it exceeds 90% by weight, there are problems with off-flavor and stability. there is.
또한, 본 발명의 하이드로졸과 추출물로 구성된 천연 추출 복합물은 화장료 조성물에 유효성분으로 함유되며, 화장료 조성물은 발향이 가능하고 미백, 주름개선 효능, UVB에 의한 세포 사멸에서 인간유래 피부각질형성세포(Human Epidermal keratinocyte)를 보호하는 항노화 효능을 갖는 것을 특징으로 한다.In addition, the natural extract complex consisting of the hydrosol and extract of the present invention is contained as an active ingredient in the cosmetic composition, and the cosmetic composition is capable of scenting and has whitening and wrinkle improvement effects, as well as human-derived skin keratinocytes in cell death caused by UVB. It is characterized by having an anti-aging effect that protects human epidermal keratinocytes.
또한, 본 발명에 따른 천연 추출 복합물은, 화장료 조성물 총 중량대비 5중량% 내지 10중량%의 범위로 함유되는 것을 특징으로 한다. 5중량% 미만인 경우에는 뇌파의 안정화에 의한 스트레스 발생시 생성되는 베타파 감소를 통한 스트레스 완화의 심리적 안정의 효과를 기대할 수 없고, 10중량%를 초과할 경우 이취 및 안정도상 변취에 대한 문제점이 있다.In addition, the natural extract complex according to the present invention is characterized in that it is contained in the range of 5% by weight to 10% by weight based on the total weight of the cosmetic composition. If it is less than 5% by weight, the effect of psychological stability in stress relief through reduction of beta waves generated when stress occurs by stabilizing brain waves cannot be expected, and if it exceeds 10% by weight, there are problems with off-flavor and stability.
또한, 본 발명에 따른 천연 추출 복합물 제조방법은,In addition, the method for producing a natural extraction complex according to the present invention,
(S1)발향 천연 식물을 이용한 하이드로졸 제조단계;(S1) Hydrosol manufacturing step using scented natural plants;
(S2)발향 천연 식물을 이용한 추출물 제조단계; (S2) Extract manufacturing step using fragrant natural plants;
(S3)상기 (S1) 및 (S2) 단계를 통해 각각 제조된 하이드로졸과 추출물을 프로판다이올, 부틸렌글라이콜, 펜틸렌글라이콜, 1,2-헥산다이올, 에틸헥실글리세린으로부터 선택된 1개 이상의 용매에서 교반 혼합하는 단계;를 포함하여 구성되는 것을 특징으로 한다.(S3) The hydrosol and extract prepared through steps (S1) and (S2) above were mixed with 1 selected from propanediol, butylene glycol, pentylene glycol, 1,2-hexanediol, and ethylhexylglycerin. It is characterized in that it comprises a step of stirring and mixing in more than one solvent.
여기서, 상기 (S1) 단계는, Here, the step (S1) is,
발향 천연 식물을 일정한 크기로 세절 및 세척하여 건조하고,Scented natural plants are cut into pieces, washed and dried.
건조한 식물에 증류수를 넣어 수증기 증류 추출장치에서 고온 추출을 한 다음,After adding distilled water to the dried plant and performing high-temperature extraction in a steam distillation extraction device,
오일이 제거된 증류액을 응축하여 고유의 특이 향취를 포함하는 하이드로졸을 수득하는 단계로 제조되며,It is manufactured by condensing the distillate from which the oil has been removed to obtain a hydrosol containing a unique, unique flavor.
제조된 하이드로졸은 프로판다이올, 부틸렌글라이콜, 펜틸렌글라이콜, 1,2-헥산다이올, 에틸헥실글리세린으로 구성된 군으로부터 선택된 하나 이상의 용제를 이용하여 여과시키는 것을 특징으로 한다.The prepared hydrosol is characterized by being filtered using one or more solvents selected from the group consisting of propanediol, butylene glycol, pentylene glycol, 1,2-hexanediol, and ethylhexylglycerin.
또한, 상기 (S2) 단계는,In addition, the step (S2) is,
발향 천연 식물을 일정한 크기로 세절 및 세척하여 건조하고,Scented natural plants are cut into pieces, washed and dried.
건조한 식물을 정제수, 에탄올, 부틸렌글라이콜 및 프로판다이올, 펜틸렌글라이콜로 구성된 군으로부터 선택된 하나 이상의 용매에 투입하여 40 oC - 100 oC 사이의 온도에서 추출하여 고유의 특이 향취를 포함하는 추출물을 수득하는 단계로 제조되며, 제조된 추출물은 저온에서 냉침하여 여과시키는 것을 특징으로 한다.The dried plant is added to one or more solvents selected from the group consisting of purified water, ethanol, butylene glycol, propanediol, and pentylene glycol, and extracted at a temperature between 40 o C and 100 o C to produce a product containing its own specific scent. It is prepared through the step of obtaining an extract, and the prepared extract is characterized by cold soaking at low temperature and filtering.
본 발명에 따라 제조된 천연 추출 복합물은 하이드로졸과 추출물을 유효성분으로 포함하며 제1형 Procollagen(Procollagen Type 1) 합성 증가, UVB 노출에 의해 감소된 세포의 생존율 회복, α-MSH에 의해 유도된 멜라닌생성을 감소시키는 항노화 효과를 나타내어 피부상태 개선과 화장료 조성물에 향을 내기 위한 목적에 유용한 효과가 있다.The natural extract complex prepared according to the present invention contains hydrosol and extract as active ingredients and increases the synthesis of Procollagen Type 1, restores the survival rate of cells reduced by UVB exposure, and increases the It has an anti-aging effect that reduces melanin production, which is useful for improving skin condition and adding fragrance to cosmetic compositions.
도 1은 본 발명에 따른 천연 추출 복합물을 UVB를 일정시간 쬐어주어 세포사멸이 유도된 인간유래 피부각질형성세포인 HaCaT에 농도별로 처리하여 세포의 생존율을 측정하고, 이를 함유한 화장료 조성물이 UVB 환경에서 세포 보호 효과를 가지는지 나타낸 도면.
도 2는 본 발명에 따른 천연 추출 복합물과 양성 대조군(positive control)인 TGF-β를 인간 진피섬유아세포(Human dermal fibroblast)에 농도별로 처리하여 제1형 프로콜라겐(Procollagen Type 1) Elisa kit를 통하여 제1형 프로콜라겐(procollagen) 합성능 변화를 나타낸 도면.
도 3은 생쥐 흑색종세포(Murine melanoma cell)인 B16F10에 α-MSH를 처리하여 멜라닌 합성을 유도하고 기존에 멜라닌 합성을 억제한다고 알려져 있는 알부틴(Arbutin)과 코직산(Kojic acid)을 처리하여 비교군을 설정한 뒤, 본 발명에 따른 천연 추출 복합물을 농도별로 처리하여 해당 물질이 멜라닌합성 저해능이 있는지 나타낸 도면.Figure 1 shows the treatment of the natural extract complex according to the present invention to HaCaT, a human-derived skin keratinocyte cell in which apoptosis was induced by exposure to UVB for a certain period of time, at different concentrations, and the survival rate of the cells was measured, and the cosmetic composition containing the same was tested in a UVB environment. A diagram showing whether it has a cytoprotective effect.
Figure 2 shows the treatment of the natural extract complex according to the present invention and TGF-β, a positive control, to human dermal fibroblasts at different concentrations to obtain type 1 procollagen (Procollagen Type 1) Elisa kit. Diagram showing changes in type 1 procollagen synthesis ability.
Figure 3 shows a comparison group in which B16F10, a murine melanoma cell, was treated with α-MSH to induce melanin synthesis and treated with arbutin and kojic acid, which are known to inhibit melanin synthesis. A diagram showing whether the natural extract complex according to the present invention has the ability to inhibit melanin synthesis by processing the natural extract complex according to the present invention by concentration.
이하, 본 발명의 바람직한 실시예의 상세한 설명은 첨부된 도면들을 참조하여 설명할 것이다. 그러나 본 발명은 여기서 설명되는 실시예들에 한정되지 않고 다른 형태로 구체화될 수도 있다. 오히려, 여기서 소개되는 실시예들은 개시된 내용이 철저하고 완전해질 수 있도록, 그리고 당업자에게 본 발명의 사상이 충분히 전달될 수 있도록 하기 위해 제공되는 것이다. 하기에서 본 발명을 설명함에 있어서, 관련된 공지 기능 또는 구성에 대한 구체적인 설명이 본 발명의 요지를 불필요하게 흐릴 수 있다고 판단되는 경우에는 그 상세한 설명을 생략할 것이다.Hereinafter, a detailed description of preferred embodiments of the present invention will be described with reference to the attached drawings. However, the present invention is not limited to the embodiments described herein and may be embodied in other forms. Rather, the embodiments introduced herein are provided so that the disclosure will be thorough and complete, and so that the spirit of the invention can be sufficiently conveyed to those skilled in the art. In the following description of the present invention, if a detailed description of a related known function or configuration is judged to unnecessarily obscure the gist of the present invention, the detailed description will be omitted.
<실시예 1> 천연 추출 복합물의 제조<Example 1> Preparation of natural extraction complex
<실시예 1-1> 하이드로졸(hydrosol(Floral Water)) 제조<Example 1-1> Hydrosol (Floral Water) production
하이드로졸(hydrosol)을 제조하기 위해, 발향 천연 식물인 라임수, 비터오렌지꽃수, 오렌지꽃수, 오렌지수, 왕귤수, 레몬수, 만다린수, 편백수, 솔잣나무잎수, 두송열매수, 구주소나무잎수, 선백리향잎수, 동백꽃수, 귤꽃수, 위치하젤잎수, 위치하젤수, 위치하젤꽃수, 에버라스팅꽃수, 쟈스민꽃수, 센티드제라늄꽃수, 센티드제라늄수, 서양장미꽃수, 다마스크장미꽃수, 파인애플수, 코코넛수, 라즈베리수, 캐모마일꽃수, 애엽수, 카렌둘라꽃수, 수레국화꽃수, 병풀잎수, 캐모마일꽃수, 유칼립투스잎수, 라벤더꽃수, 라벤더수, 라반딘꽃수, 레몬버베나잎수, 레몬버베나수, 레몬밤꽃과 잎 및 줄기수, 로즈마리잎수, 세이지잎수, 세이지수, 클라리꽃과 잎 및 줄기수, 페퍼민트잎수, 티트리잎수, 회향수, 팔각회향수, 인삼수, 바닐라열매수, 피나무꽃수로 이루어진 군으로부터 선택된 어느 하나 이상을 선택하여 꽃잎, 잎사귀, 줄기, 뿌리 등 적절한 부위를 수득하고, 이를 일정한 크기로 세절 및 세척하여 건조한다. To manufacture hydrosol, fragrant natural plants such as lime water, bitter orange flower water, orange flower water, orange water, tangerine water, lemon water, mandarin water, cypress water, pine pine leaf water, pine tree fruit water, and pine tree leaf water are used. , thyme leaf water, camellia flower water, tangerine flower water, witch hazel leaf water, witch hazel water, witch hazel flower water, everlasting flower water, jasmine flower water, scented geranium flower water, scented geranium water, western rose flower water, damask rose flower water, pineapple Water, coconut water, raspberry water, chamomile flower water, petal leaf water, calendula flower water, cornflower flower water, centella leaf water, chamomile flower water, eucalyptus leaf water, lavender flower water, lavender water, lavandin flower water, lemon verbena leaf water, lemon verbena water, Consisting of lemon balm flowers, leaves and stem water, rosemary leaf water, sage leaf water, sage water, clary flower and leaf and stem water, peppermint leaf water, tea tree leaf water, fennel water, star aniseed fennel water, ginseng water, vanilla fruit water and blood tree flower water. Select one or more selected from the group to obtain appropriate parts such as petals, leaves, stems, and roots, and cut them into pieces of a certain size, wash them, and dry them.
건조한 식물에 증류수를 넣어 수증기 증류 추출장치에서 100 oC 이상의 고온 추출을 하고 상층부의 오일이 제거된 증류액을 콘덴서를 통해 응축하여 고유의 특이 향취를 포함하는 하이드로졸을 수득한다. Distilled water is added to dried plants, extraction is performed at a high temperature of 100 o C or higher in a steam distillation extraction device, and the distillate from which the oil in the upper layer has been removed is condensed through a condenser to obtain a hydrosol containing its own unique scent.
하이드로졸은 보관을 위해 프로판다이올, 부틸렌글라이콜, 펜틸렌글라이콜 1,2-헥산다이올, 에틸헥실글리세린으로 구성된 군으로부터 선택된 하나 이상의 용제를 투입하여 교반하고 여과하여 보관한다.For storage, the hydrosol is stored by adding at least one solvent selected from the group consisting of propanediol, butylene glycol, pentylene glycol 1,2-hexanediol, and ethylhexylglycerin, stirring, filtering, and storing.
<실시예 1-2> 추출물(Extract)의 제조<Example 1-2> Preparation of extract
발향 천연 식물인 다마스크장미꽃봉오리추출물, 다마스크장미추출물, 다마스크장미꽃추출물, 프렌치로즈꽃추출물, 장미꽃추출물, 사향장미잎추출물, 영실추출물, 장미추출물, 코코넛추출물, 산딸기추출물, 카제푸트열매추출물, 블랙베리추출물, 블랙베리잎추출물, 장딸기열매/잎추출물, 라즈베리추출물, 라즈베리잎추출물, 멍석딸기추출물, 블루베리추출물, 블루베리잎추출물, 하이부쉬블루베리추출물, 덩굴월귤열매추출물, 서양톱풀추출물, 서양톱풀꽃추출물, 아그리모니추출물, 아그리모니잎추출물, 캐모마일꽃추출물, 캐모마일꽃수, 쓴쑥추출물, 청호추출물, 유기노추출물, 사철쑥꽃추출물, 쑥잎추출물, 타라곤잎/줄기추출물, 타라곤뿌리추출물, 요모기잎추출물, 쑥캘러스추출물, 애엽추출물, 애엽수, 알파인웜우드추출물, 쑥추출물, 불가리스쑥과 슈크로오스발효추출물, 카렌둘라추출물, 카렌둘라꽃추출물, 수레국화꽃추출물, 병풀추출물, 병풀잎추출물, 병풀잎수, 캐모마일추출물, 캐모마일꽃추출물, 캐모마일꽃과 잎추출물, 캐모마일꽃과 잎 및 줄기추출물, 캐모마일잎추출물, 국화꽃추출물, 레몬그라스추출물, 레몬그라스추출물, 레몬그라스잎추출물, 레몬그라스잎과 줄기추출물, 유칼립투스잎추출물, 좁은잎페퍼민트검꽃과 잎 및 줄기추출물, 히솝추출물, 라벤더추출물, 라벤더꽃추출물, 라벤더꽃과 잎 및 줄기추출물, 라반딘꽃추출물, 더치라벤더꽃과 잎 및 줄기추출물, 프렌치라벤더추출물, 레몬버베나꽃추출물, 레몬버베나잎추출물, 레몬버베나잎수, 레몬밤추출물, 레몬밤꽃과 잎 및 줄기추출물, 레몬밤잎추출물, 로즈마리추출물, 로즈마리꽃추출물, 로즈마리잎추출물, 세이지추출물, 세이지잎추출물, 베르가못민트잎추출물, 박하추출물, 박하꽃과 잎 및 줄기추출물, 박하잎추출물, 콘민트추출물, 페퍼민트캘러스배양추출물, 페퍼민트추출물, 페퍼민트꽃과 잎 및 줄기추출물, 페퍼민트잎추출물, 페니로얄추출물, 애플민트잎추출물, 양박하꽃과 잎 및 줄기추출물, 스피어민트추출물, 스피어민트잎추출물, 서양고추나물추출물, 서양고추나물꽃과 잎 및 줄기추출물, 티트리추출물, 티트리꽃과 잎 및 줄기추출물, 티트리잎추출물, 회향추출물, 회향잎추출물, 회향뿌리추출물, 회향씨추출물, 스파이크드진저릴리추출물, 팔각회향추출물, 월계수잎추출물, 오레가노꽃추출물, 오레가노잎추출물, 오레가노잎과 잎 및 줄기추출물, 인삼열매추출물, 인삼캘러스배양추출물, 인삼형성층세포배양추출물, 인삼꽃추출물, 인삼잎과 줄기추출물, 산삼배양근추출물, 인삼씨추출물, 인삼싹추출물, 바닐라콩추출물, 바닐라꽃추출물, 바닐라열매추출물, 발삼캐나다추출물, 로즈우드추출물, 침향추출물, 서던우드추출물, 위치하젤껍질과 잎추출물, 위치하젤껍질과 잎 및 잔가지추출물, 위치하젤껍질과 잔가지추출물, 백단향목부추출물, 피나무추출물, 피나무꽃추출물, 피나무추출물, 헬리크리섬꽃추출물로 이루어진 군으로부터 선택된 어느 하나 이상을 적절한 부위를 수득하여, 일정한 크기로 세절 및 세척하여 건조한다.Fragrant natural plants such as damask rose bud extract, damask rose extract, damask rose flower extract, French rose flower extract, rose flower extract, musk rose leaf extract, youngsil extract, rose extract, coconut extract, raspberry extract, and kajeput. Fruit extract, blackberry extract, blackberry leaf extract, long strawberry fruit/leaf extract, raspberry extract, raspberry leaf extract, strawberry extract, blueberry extract, blueberry leaf extract, highbush blueberry extract, lingonberry fruit extract, Western Yarrow extract, yarrow flower extract, agrimony extract, agrimony leaf extract, chamomile flower extract, chamomile flower water, bitter mugwort extract, green tiger extract, organino extract, mugwort flower extract, mugwort leaf extract, tarragon leaf/stem extract, tarragon Root extract, quince leaf extract, mugwort callus extract, leaf extract, leaf extract, alpine wormwood extract, mugwort extract, vulgaris mugwort and sucrose fermentation extract, calendula extract, calendula flower extract, cornflower flower extract, centella asiatica extract , Centella asiatica leaf extract, Centella asiatica leaf water, chamomile extract, chamomile flower extract, chamomile flower and leaf extract, chamomile flower, leaf and stem extract, chamomile leaf extract, chrysanthemum flower extract, lemongrass extract, lemongrass extract, lemongrass leaf extract , lemongrass leaf and stem extract, eucalyptus leaf extract, narrow-leaf peppermint gum flower and leaf and stem extract, hyssop extract, lavender extract, lavender flower extract, lavender flower, leaf and stem extract, lavandin flower extract, Dutch lavender flower and leaf, and Stem extract, French lavender extract, lemon verbena flower extract, lemon verbena leaf extract, lemon verbena leaf water, lemon balm extract, lemon balm flower, leaf and stem extract, lemon balm leaf extract, rosemary extract, rosemary flower extract, rosemary leaf extract, sage extract , sage leaf extract, bergamot mint leaf extract, peppermint extract, peppermint flower, leaf and stem extract, peppermint leaf extract, corn mint extract, peppermint callus culture extract, peppermint extract, peppermint flower, leaf and stem extract, peppermint leaf extract, penny Royal extract, apple mint leaf extract, spearmint flower, leaf and stem extract, spearmint extract, spearmint leaf extract, pepper plant extract, pepper plant flower, leaf and stem extract, tea tree extract, tea tree flower, leaf and stem Extract, tea tree leaf extract, fennel extract, fennel leaf extract, fennel root extract, fennel seed extract, spiked ginger lily extract, star anise fennel extract, bay leaf extract, oregano flower extract, oregano leaf extract, oregano leaf and leaves and stem Extract, ginseng fruit extract, ginseng callus culture extract, ginseng cambium cell culture extract, ginseng flower extract, ginseng leaf and stem extract, wild ginseng cultured root extract, ginseng seed extract, ginseng bud extract, vanilla bean extract, vanilla flower extract, vanilla fruit extract , Balsam Canada extract, rosewood extract, agarwood extract, southernwood extract, witch hazel bark and leaf extract, witch hazel bark, leaf and twig extract, witch hazel bark and twig extract, sandalwood xylem extract, blood tree extract, blood tree flower extract, Obtain an appropriate part of one or more selected from the group consisting of a blood tree extract and a helichrysum flower extract, cut them into pieces of a certain size, wash them, and dry them.
건조된 발향 천연 식물에 정제수, 에탄올, 부틸렌글라이콜 및 프로판다이올, 펜틸렌글라이콜로 구성된 군으로부터 선택된 하나 이상의 용매에 투입하여 40 oC - 100 oC 사이의 온도에서 추출하여 고유의 특이 향취를 포함하는 추출물을 수득한다. 수득한 추출물은 저온에서 냉침하여 여과한다.Dried natural scented plants are added to one or more solvents selected from the group consisting of purified water, ethanol, butylene glycol, propanediol, and pentylene glycol, and extracted at a temperature between 40 o C and 100 o C to produce a unique, unique scent. An extract containing is obtained. The obtained extract is cold-soaked at low temperature and filtered.
<실시예 1-3> 하이드로졸(Hydrosol(Floral Water))과 추출물(Extract)을 유효성분으로 하는 천연 추출 복합물 제조<Example 1-3> Preparation of a natural extract composite containing Hydrosol (Floral Water) and Extract as active ingredients
상기 실시예1-1 및 실시예1-2를 통해 각각 제조되어 선택된 하나 이상의 하이드로졸과 천연물을 프로판다이올, 부틸렌글라이콜, 펜틸렌글라이콜, 1,2-헥산다이올, 에틸헥실글리세린으로부터 선택된 1개 이상의 용매에서 상온에서 교반하여 혼합한다. One or more hydrosols and natural products prepared and selected through Examples 1-1 and 1-2 above were mixed with propanediol, butylene glycol, pentylene glycol, 1,2-hexanediol, and ethylhexylglycerin. Mix by stirring at room temperature in one or more solvents selected from.
하이드로졸 및 추출물은, 천연 추출 복합물 총 중량대비 60중량% 내지 90중량%의 범위로 포함된다. 60중량% 미만인 경우에는 뇌파의 안정화에 의한 스트레스 발생시 생성되는 베타파 감소를 통한 스트레스 완화의 심리적 안정의 효과를 기대할 수 없고, 90중량%를 초과할 경우 이취 및 안정도상 변취에 대한 문제점이 있다.Hydrosols and extracts are included in the range of 60% to 90% by weight based on the total weight of the naturally extracted complex. If it is less than 60% by weight, the effect of psychological stability in stress relief through reduction of beta waves generated when stress occurs by stabilizing brain waves cannot be expected, and if it exceeds 90% by weight, there are problems with off-flavor and stability.
<실험예 1 및 결과> 재료 및 실험방법<Experimental Example 1 and Results> Materials and Experimental Methods
<재료><Material>
세포배양에 필요한 Dulbecco Modified Eagle Medium(DMEM)과 소태아혈청(Fetal bovine serum, FBS), 트립신-EDTA(Trypsin-EDTA), Dulbecco's Phosphate Buffered Saline(DPBS)은 미국의 Gibco사에서 구매하여 사용하였다. Fibroblast Basal Medium(FBM), FGM-2 SingleQuot Kit Supplement & Growth Factors는 LONZA에서 구매 하였으며 세포생존율 측정 Kit는 한국의 두젠바이오(Dogen)에서 구매하여 사용하였다. 제1형 프로콜라겐(Procollagen Type 1) 측정 kit는 일본의 Takara에서 구매하여 이용하였다. α-MSH, 알부틴(Arbutin), 코직산(Kojic acid)은 Sigma-Aldrich로부터 구매하여 사용하였다.Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), Trypsin-EDTA, and Dulbecco's Phosphate Buffered Saline (DPBS) required for cell culture were purchased from Gibco in the United States. Fibroblast Basal Medium (FBM), FGM-2 SingleQuot Kit Supplement & Growth Factors were purchased from LONZA, and the cell viability measurement kit was purchased from Dogen in Korea. The Procollagen Type 1 measurement kit was purchased and used from Takara, Japan. α-MSH, Arbutin, and Kojic acid were purchased from Sigma-Aldrich.
<세포배양><Cell culture>
인간유래 피부각질형성세포인 HaCaT, 생쥐 흑색종세포인 B16F10을 페니실린(100 IU/mL), 생쥐 대식세포(Murine macrophage)인 RAW 264.7을 스트렙토마이신(100 ug/mL), 10% FBS (fetal bovine serum)를 함유하는 DMEM (Dulbecco's Modified Eagle's Medium) 배지를 넣고 37℃, 5% 이산화탄소를 포함하는 배양기 내에서 배양하였다. 인간 진피섬유아세포를 페니실린(100 IU/mL), 스트렙토마이신(100 ug/mL), 보조제(supplement)를 함유하는 Fibroblast Basal Medium(FBM) 배지를 넣고 37℃, 5% 이산화탄소를 포함하는 배양기내에서 배양하였다.HaCaT, a human-derived skin keratinocyte, B16F10, a mouse melanoma cell, with penicillin (100 IU/mL), and RAW 264.7, a murine macrophage, with streptomycin (100 ug/mL) and 10% FBS (fetal bovine). DMEM (Dulbecco's Modified Eagle's Medium) containing serum was added and cultured in an incubator containing 5% carbon dioxide at 37°C. Human dermal fibroblasts were cultured in Fibroblast Basal Medium (FBM) containing penicillin (100 IU/mL), streptomycin (100 ug/mL), and supplements in an incubator containing 5% carbon dioxide at 37°C. Cultured.
<실험예 2 및 결과> 천연 추출 복합물의 세포 독성 평가<Experimental Example 2 and Results> Cytotoxicity evaluation of natural extract complexes
(WST-1 assay) 96well plate에 인간 진피섬유아세포를 6 × 103cells/well씩, HaCaT을 1.5 × 104cells/well씩, RAW 264.7을 6 × 104cells/well씩, B16F10을 1.5 × 104cells/well씩 분주한 후, 세포 배양조건에서 배양하였다. 24시간 후, 배양액을 버리고 PBS로 세척한 다음 인간 진피섬유아세포는 보조제를 함유하지 않은 FBM 배지를, HaCaT, B16F10, RAW264.7 세포는 FBS를 함유하지 않은 DMEM 배지를 사용하여 세포를 기아상태로 만들어주었다. 다음 날, 일정 농도의 시험물질을 처리하여 24시간 배양하였다. 배지에 10배 희석시킨 WST-1 시약을 각 well에 100 ul씩 넣고 2시간 배양 후, 450nm에서 흡광도를 측정하였다. (WST-1 assay) In a 96 -well plate, human dermal fibroblasts were added at 6 After dispensing 10 4 cells/well, the cells were cultured under cell culture conditions. After 24 hours, the culture medium was discarded, washed with PBS, and the cells were starved using FBM medium without supplements for human dermal fibroblasts and DMEM medium without FBS for HaCaT, B16F10, and RAW264.7 cells. I made it. The next day, the test substances were treated with a certain concentration and cultured for 24 hours. 100 ul of WST-1 reagent diluted 10 times in medium was added to each well, and after 2 hours of incubation, the absorbance was measured at 450 nm.
실험결과는 대조군(control)과 비교하여 각각의 세포마다 독성이 나타나는 지점이 다르다는 것을 확인할 수 있다. RAW 264.7에서는 낮은 시료예 1의 농도인 2.5%에서도 세포가 20% 사멸하나 인간 진피섬유아세포 HaCaT에서는 시료예 1의 2.5% 처리에서도 큰 세포독성은 나타나지 않았다. B16F10에서는 시료예 1의 10% 처리에서도 세포사멸이 나타나지 않는 것을 확인할 수 있다. 이러한 각각의 세포 생존율을 수치화한 결과는 표 2에 나타내었다. The experimental results show that the point where toxicity appears for each cell is different compared to the control. In RAW 264.7, 20% of cells died even at 2.5%, which is the low concentration of Sample Example 1, but in human dermal fibroblast HaCaT, no significant cytotoxicity was observed even at 2.5% of Sample Example 1. In B16F10, it can be seen that cell death does not occur even when treated with 10% of Sample Example 1. The results quantifying each cell survival rate are shown in Table 2.
<실험예 3 및 결과> 인간유래 피부각질형성세포의 UVB 노출환경에서 천연 추출 복합물에 의한 세포 보호 효과세포보호 평가 (WST-1 assay) 96well plate에 HaCaT을 1.5 × 104cells/well씩 분주한 후, 세포 배양조건에서 배양하였다. 24시간 후, 배양액을 버리고 PBS로 세척한 다음 FBS를 함유하지 않은 DMEM 배지를 사용하여 세포를 기아상태로 만들어주었다. 다음 날, 일정량의 UVB 조사 후 일정 농도의 시험물질을 처리하여 24시간 배양하였다. 배지에 10배 희석시킨 WST-1 시약을 각 well에 100 ul씩 넣고 2시간 배양 후, 450nm에서 흡광도를 측정하였다.<Experimental Example 3 and Results> Evaluation of cell protection effect by natural extract complex in UVB exposure environment of human-derived skin keratinocytes (WST-1 assay) HaCaT was divided into 1.5 × 10 4 cells/well in a 96-well plate. Afterwards, the cells were cultured under cell culture conditions. After 24 hours, the culture medium was discarded, washed with PBS, and the cells were starved using DMEM medium without FBS. The next day, after a certain amount of UVB irradiation, the test substances were treated with a certain concentration and cultured for 24 hours. 100 ul of WST-1 reagent diluted 10 times in medium was added to each well, and after 2 hours of incubation, the absorbance was measured at 450 nm.
실험결과는 UVB 처리에 의한 생존율이 약 17% 감소하였던 HaCaT 세포의 생존율이 제공된 시료예 1의 처리 농도에 따라 세포의 생존율이 증가하는 것을 확인하였다. 특히 시료예 1의 1% 처리는 UVB에 의해 감소되었던 세포 생존율을 12% 회복시키는 것으로 나타났다. HaCaT 세포의 생존율 변화는 표 3과 도 1에 자세하게 나타내었다.The experimental results confirmed that the survival rate of HaCaT cells, which had decreased by about 17% due to UVB treatment, increased depending on the treatment concentration of Sample Example 1. In particular, the 1% treatment of Sample Example 1 was found to restore the cell viability that had been reduced by UVB by 12%. Changes in the survival rate of HaCaT cells are shown in detail in Table 3 and Figure 1.
<실험예 4 및 결과> 인간 진피섬유아세포의 천연 추출 복합물에 의한 제1형 프로콜라겐(Procollagen Type 1) 생성 촉진 효과제1형 프로콜라겐 생성능 평가를 위해 96well plate에 인간 진피섬유아세포를 6 × 103cells/well씩 분주한 후, 세포 배양조건에서 배양하였다. 24시간 후, 배양액을 버리고 PBS로 세척한 다음 보조제를 함유하지 않은 FBM 배지를 사용하여 세포를 기아상태로 만들어주었다. 다음 날, 일정 농도의 시험물질을 처리하여 24시간 배양하였다. 제1형 프로콜라겐 Elisa kit를 이용하 여 실험 후, 450nm에서 흡광도를 측정하였다. 최종 제1형 프로콜라겐의 양은 일정 단백질 당 제1형 프로콜라겐의 양으로 환산하여 음성대조군과 비교하였다. <Experimental Example 4 and Results> Effect of promoting type 1 procollagen (Procollagen Type 1) production by natural extract complex of human dermal fibroblasts To evaluate the ability to produce type 1 procollagen, 6 × 10 human dermal fibroblasts were placed in a 96-well plate. After dispensing 3 cells/well, the cells were cultured under cell culture conditions. After 24 hours, the culture medium was discarded, washed with PBS, and the cells were starved using FBM medium without supplements. The next day, the test substances were treated with a certain concentration and cultured for 24 hours. After the experiment using the type 1 procollagen Elisa kit, the absorbance was measured at 450 nm. The final amount of type 1 procollagen was converted to the amount of type 1 procollagen per certain protein and compared with the negative control group.
실험결과는 시료예 1의 처리 농도에 따라 농도의존적으로 제1형 클로콜라겐 생성이 증가하였으며 0.4%의 농도에서 최고 60.8% 증가하는 것으로 나타났다. 자세한 제1형 프로콜라겐 생성량 변화는 표 4와 도 2에 나타내었다.The experimental results showed that the production of type 1 clolagen increased in a concentration-dependent manner depending on the treatment concentration of Sample Example 1, with a maximum increase of 60.8% at a concentration of 0.4%. Detailed changes in type 1 procollagen production are shown in Table 4 and Figure 2.
<실험예 5 및 결과> 천연 추출 복합물에 의한 멜라닌 생성 억제능 확인Melanin assay 24well plate에 B16F10을 2 × 104cells/well씩 분주한 후, 세포 배양조건에서 배 양하였다. 24시간 후, 멜라닌 생성 과정을 촉진하는 α-MSH 100 nM과 함께 일정 농도의 시험물질을 처리하여 72시간 배양하였다. 72시간 후, 세포 배양액을 immunoplate에 옮겨 세포 외 멜라닌(Release melanin)의 양을 405nm에서 흡광도를 측정하였다. 배지를 제거하고 남은 세포는 PBS로 세척 후, 1N의 수산화나트륨을 100 ul를 넣고 용해시켰다. 용해시켜 얻은 세포 내 멜라닌(Cellular melanin)의 양을 405nm에서 흡광도를 측정하였다. 세포 외 멜라닌 양과 세포 내 멜라닌 양을 일정 단백질 당 멜라닌 양으로 환산하여 음성대조군과 비교하였다.<Experimental Example 5 and Results> Confirmation of melanin production inhibition ability by natural extract complex Melanin assay B16F10 was dispensed at 2 × 104 cells/well into a 24-well plate and cultured under cell culture conditions. After 24 hours, a certain concentration of test substance was treated with 100 nM of α-MSH, which promotes the melanin production process, and cultured for 72 hours. After 72 hours, the cell culture was transferred to an immunoplate, and the amount of extracellular melanin (release melanin) was measured by absorbance at 405 nm. After removing the medium, the remaining cells were washed with PBS and dissolved by adding 100 ul of 1N sodium hydroxide. The amount of intracellular melanin obtained by dissolution was measured by absorbance at 405 nm. The amount of extracellular melanin and intracellular melanin were converted to the amount of melanin per certain protein and compared with the negative control group.
실험결과 α-MSH에 의해 175.1% 증가한 멜라닌 생성량이 시료예 1의 5% 농도에서 최고 136.9% 감소하였다. 멜라닌 생성량은 표 5와 도 3에 자세하게 나타냈다. As a result of the experiment, the amount of melanin production, which increased by 175.1% by α-MSH, decreased by up to 136.9% at 5% concentration in Sample Example 1. The amount of melanin produced is shown in detail in Table 5 and Figure 3.
시료예1 (%)
Sample Example 1 (%)
상술한 바와 같이, 본 발명에 따른 천연 추출 복합물은 하이드로졸(hydrosol)과 추출물(extract)을 유효성분으로 포함함으로써, 제1형 프로콜라겐 합성 증가, UVB 노출에 의해 감소된 세포의 생존율 회복, α-MSH에 의해 유도된 멜라닌생성을 감소시키는 항노화 효과를 나타내어 피부상태 개선에 효과가 있음을 확인할 수 있었다. 이하, 본 발명에 따른 천연 추출 복합물을 함유하는 화장료 조성물의 제형예를 설명하나, 다른 여러 가지 제형으로 응용 가능함은 물론이다.As described above, the natural extract complex according to the present invention contains hydrosol and extract as active ingredients, thereby increasing type 1 procollagen synthesis, recovering cell survival rate reduced by UVB exposure, and α -It was confirmed that it has an anti-aging effect by reducing melanin production induced by MSH and is effective in improving skin condition. Hereinafter, formulation examples of the cosmetic composition containing the natural extract complex according to the present invention will be described, but of course, it can be applied to various other formulations.
<제형예 1> 유연화장수(스킨토너)<Formulation example 1> Soft lotion (skin toner)
하기 표 6에 따른 함량으로 통상적인 방법으로 유연화장수를 제조하였다. 본 발명에 따른 천연 추출 복합물(시료예 1)이 향을 대체하므로 따로 가용화제가 첨가되지 않아도 투명한 형태의 유연화장수를 제조할 수 있다.Soft lotion was prepared in a conventional manner with the content according to Table 6 below. Since the natural extract complex (Sample Example 1) according to the present invention replaces the fragrance, a transparent soft lotion can be produced without adding a separate solubilizer.
<제형예 2> 젤크림(수분크림)하기 표 7에 따른 함량으로 통상적인 방법으로 젤크림을 제조하였다. 본 발명에 따른 천연 추출 복합물(시료예 1)이 향을 대체하므로 따로 가용화제가 첨가되지 않아도 투명한 형태의 젤크림을 제조할 수 있다.<Formulation Example 2> Gel cream (moisture cream) Gel cream was prepared by a conventional method with the content according to Table 7 below. Since the natural extract complex according to the present invention (Sample Example 1) replaces the fragrance, a transparent gel cream can be manufactured without adding a separate solubilizer.
<제형예 3> 크림하기 표 8에 기재된 조성에 따라 통상적인 방법으로 크림을 제조하였다. 본 발명에 따른 천연 추출 복합물 (시료예 1)이 향료를 대체하는 바 제형내에 착향제로 분류되는 성분은 포함되지 않는다.<Formulation Example 3> Cream A cream was prepared by a conventional method according to the composition shown in Table 8. The natural extract complex according to the present invention (Sample Example 1) replaces fragrance, and does not contain ingredients classified as flavoring agents in the formulation.
여기서, 본 발명에 따른 천연 추출 복합물은, 화장료 조성물 총 중량대비 5중량% 내지 10중량%의 범위로 포함된다. 5중량% 미만인 경우에는 뇌파의 안정화에 의한 스트레스 발생시 생성되는 베타파 감소를 통한 스트레스 완화의 심리적 안정의 효과를 기대할 수 없고, 10중량%를 초과할 경우 이취 및 안정도상 변취에 대한 문제점이 있다.Here, the natural extract complex according to the present invention is contained in the range of 5% to 10% by weight based on the total weight of the cosmetic composition. If it is less than 5% by weight, the effect of psychological stability in stress relief through reduction of beta waves generated when stress occurs by stabilizing brain waves cannot be expected, and if it exceeds 10% by weight, there are problems with off-flavor and stability.
상기에서는 본 발명의 일 실시예를 참조하여 설명하였지만, 해당 기술분야의 당업자는 이하에서 서술하는 특허 청구범위에 기재된 본 발명의 사상 및 영역으로부터 벗어나지 않는 범위 내에서 본 발명을 다양하게 수정 및 변경 실시할 수 있을 것이다. 그러므로 변형된 실시가 기본적으로 본 발명의 특허청구범위의 구성 요소를 포함한다면 모두 본 발명의 기술적 범주에 포함된다고 보아야 한다.Although the present invention has been described above with reference to an embodiment of the present invention, those skilled in the art may make various modifications and changes to the present invention without departing from the spirit and scope of the present invention as set forth in the patent claims described below. You can do it. Therefore, if the modified implementation basically includes the elements of the claims of the present invention, it should be considered to be included in the technical scope of the present invention.
Claims (3)
(S2) 발향 천연 식물을 이용한 추출물 제조단계;
(S3) 상기 (S1) 및 (S2) 단계를 통해 각각 제조된 하이드로졸과 추출물을 프로판다이올, 부틸렌글라이콜, 펜틸렌글라이콜, 1,2-헥산다이올, 에틸헥실글리세린으로부터 선택된 1개 이상의 용매에서 교반 혼합하는 단계;를 포함하고,
상기 (S1) 단계는,
발향 천연 식물을 일정한 크기로 세절 및 세척하여 건조하고,
건조한 식물에 증류수를 넣어 수증기 증류 추출장치에서 고온 추출을 한 다음,
오일이 제거된 증류액을 응축하여 특이 향취를 포함하는 하이드로졸을 수득하는 단계로 제조되며,
제조된 하이드로졸은 보관을 위해 프로판다이올, 부틸렌글라이콜, 펜틸렌글라이콜, 1,2-헥산다이올, 에틸헥실글리세린으로 구성된 군으로부터 선택된 하나 이상의 용제를 이용하여 여과시키며,
상기 (S2) 단계는,
발향 천연 식물을 일정한 크기로 세절 및 세척하여 건조하고,
건조한 식물을 정제수, 에탄올, 부틸렌글라이콜 및 프로판다이올, 펜틸렌글라이콜로 구성된 군으로부터 선택된 하나 이상의 용매에 투입하여 40 oC - 100 oC 사이의 온도에서 추출하여 특이 향취를 포함하는 추출물을 수득하는 단계로 제조되며, 제조된 추출물은 저온에서 냉침하여 여과시키고,
상기 하이드로졸 및 추출물은, 천연 추출 복합물 총 중량대비 60중량% 내지 90중량%의 범위로 포함되고,
상기와 같이 제조된 천연 추출 복합물은 제1형 프로콜라겐 합성을 증가시키고, UVB 노출에 의해 감소된 세포의 생존율을 높이며,
다마스크장미꽃수, 라벤더꽃수, 동백나무꽃추출물, 레몬밤잎추출물, 부틸렌글라이콜, 펜틸렌글라이콜, 1,2-헥산다이올 및 정제수를 함유하는 것을 특징으로 하는 천연 추출 복합물 제조방법.(S1) Hydrosol manufacturing step using scented natural plants;
(S2) extract manufacturing step using fragrant natural plants;
(S3) The hydrosol and extract prepared through steps (S1) and (S2) respectively were mixed with 1 selected from propanediol, butylene glycol, pentylene glycol, 1,2-hexanediol, and ethylhexylglycerin. Comprising: stirring and mixing in one or more solvents,
In the step (S1),
Scented natural plants are cut into pieces, washed and dried.
After adding distilled water to the dried plant and performing high-temperature extraction in a steam distillation extraction device,
It is manufactured by condensing the distillate from which the oil has been removed to obtain a hydrosol containing a specific fragrance,
The prepared hydrosol is filtered using one or more solvents selected from the group consisting of propanediol, butylene glycol, pentylene glycol, 1,2-hexanediol, and ethylhexylglycerin for storage,
In the step (S2),
Scented natural plants are cut into pieces, washed and dried.
The dried plant is added to one or more solvents selected from the group consisting of purified water, ethanol, butylene glycol, propanediol, and pentylene glycol, and extracted at a temperature between 40 oC and 100 oC to obtain an extract containing a specific scent. It is manufactured in steps, and the prepared extract is cold-soaked at low temperature and filtered.
The hydrosol and extract are contained in the range of 60% by weight to 90% by weight based on the total weight of the natural extract complex,
The natural extract complex prepared as above increases type 1 procollagen synthesis and increases the survival rate of cells reduced by UVB exposure.
A method for manufacturing a natural extraction complex comprising damask rose flower water, lavender flower water, camellia flower extract, lemon balm leaf extract, butylene glycol, pentylene glycol, 1,2-hexanediol, and purified water.
상기 천연 추출 복합물은 화장료 조성물 총 중량대비 5중량% 내지 10중량%의 범위로 포함되는 것을 특징으로 하는 화장료 조성물.
According to paragraph 2,
A cosmetic composition characterized in that the natural extract complex is contained in the range of 5% by weight to 10% by weight based on the total weight of the cosmetic composition.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101998032B1 (en) | 2018-10-15 | 2019-07-08 | 주식회사 바이오에프디엔씨 | Cosmetic Composition for Improving Skin Condition Comprising Rosa Damascena Callus Culture Extract or the fermented filtrates to improve periorbital wrinkles, skin brightness and calm troubled skin |
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| Publication number | Publication date |
|---|---|
| KR20230149272A (en) | 2023-10-26 |
| KR20220109679A (en) | 2022-08-05 |
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