KR102660861B1 - 생체거대분자의 정제를 위한 초분자 고친화성 단백질-결합 시스템 - Google Patents

생체거대분자의 정제를 위한 초분자 고친화성 단백질-결합 시스템 Download PDF

Info

Publication number
KR102660861B1
KR102660861B1 KR1020197031323A KR20197031323A KR102660861B1 KR 102660861 B1 KR102660861 B1 KR 102660861B1 KR 1020197031323 A KR1020197031323 A KR 1020197031323A KR 20197031323 A KR20197031323 A KR 20197031323A KR 102660861 B1 KR102660861 B1 KR 102660861B1
Authority
KR
South Korea
Prior art keywords
antibody
peptide
binding
immuno
igg
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020197031323A
Other languages
English (en)
Korean (ko)
Other versions
KR20190138648A (ko
Inventor
홍강 쿠이
이 리
쑤언쿠오 쑤
라이 린 락
정지안 리
Original Assignee
더 존스 홉킨스 유니버시티
브리스톨-마이어스 스큅 컴퍼니
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 더 존스 홉킨스 유니버시티, 브리스톨-마이어스 스큅 컴퍼니 filed Critical 더 존스 홉킨스 유니버시티
Priority to KR1020237034773A priority Critical patent/KR20230148855A/ko
Publication of KR20190138648A publication Critical patent/KR20190138648A/ko
Application granted granted Critical
Publication of KR102660861B1 publication Critical patent/KR102660861B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/24—Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28014—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
    • B01J20/28023—Fibres or filaments
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/303—Extraction; Separation; Purification by precipitation by salting out
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/305—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F)
    • C07K14/31—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Micrococcaceae (F) from Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Engineering & Computer Science (AREA)
  • Water Supply & Treatment (AREA)
  • Peptides Or Proteins (AREA)
KR1020197031323A 2017-03-30 2018-03-28 생체거대분자의 정제를 위한 초분자 고친화성 단백질-결합 시스템 Active KR102660861B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1020237034773A KR20230148855A (ko) 2017-03-30 2018-03-28 생체거대분자의 정제를 위한 초분자 고친화성 단백질-결합 시스템

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762478886P 2017-03-30 2017-03-30
US62/478,886 2017-03-30
PCT/US2018/024721 WO2018183417A1 (en) 2017-03-30 2018-03-28 A supramolecular high affinity protein-binding system for purification of biomacromolecules

Related Child Applications (1)

Application Number Title Priority Date Filing Date
KR1020237034773A Division KR20230148855A (ko) 2017-03-30 2018-03-28 생체거대분자의 정제를 위한 초분자 고친화성 단백질-결합 시스템

Publications (2)

Publication Number Publication Date
KR20190138648A KR20190138648A (ko) 2019-12-13
KR102660861B1 true KR102660861B1 (ko) 2024-04-25

Family

ID=63678187

Family Applications (2)

Application Number Title Priority Date Filing Date
KR1020197031323A Active KR102660861B1 (ko) 2017-03-30 2018-03-28 생체거대분자의 정제를 위한 초분자 고친화성 단백질-결합 시스템
KR1020237034773A Ceased KR20230148855A (ko) 2017-03-30 2018-03-28 생체거대분자의 정제를 위한 초분자 고친화성 단백질-결합 시스템

Family Applications After (1)

Application Number Title Priority Date Filing Date
KR1020237034773A Ceased KR20230148855A (ko) 2017-03-30 2018-03-28 생체거대분자의 정제를 위한 초분자 고친화성 단백질-결합 시스템

Country Status (6)

Country Link
US (3) US11359005B2 (https=)
EP (1) EP3601362A4 (https=)
JP (2) JP7229169B2 (https=)
KR (2) KR102660861B1 (https=)
CN (2) CN110741017B (https=)
WO (1) WO2018183417A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3601362A4 (en) * 2017-03-30 2020-12-16 The Johns Hopkins University HIGH AFFINITY PROTEIN SUPRAMOLECULAR BINDING SYSTEM FOR BIOMACROMOLECULES PURIFICATION
JP7379323B2 (ja) * 2017-08-18 2023-11-14 ザ・ジョンズ・ホプキンス・ユニバーシティ タンパク質精製のための超分子フィラメント集合体
US20210130395A1 (en) * 2018-05-11 2021-05-06 William Marsh Rice University Proximity induced site-specific antibody conjugation
CN114225020B (zh) * 2021-12-31 2023-05-02 暨南大学 一种ova淀粉样蛋白原纤维及其制备方法与应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090214421A1 (en) 2005-05-17 2009-08-27 Sapporo-Medical University ANTIBODY DIRECTED AGAINST PAP2a AND USE THEREOF FOR DIAGNOSTIC AND THERAPEUTIC PURPOSES
US20110201112A1 (en) 2009-11-19 2011-08-18 The Regents Of The University Of California Vault Complexes for Facilitating Biomolecule Delivery

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5476996A (en) 1988-06-14 1995-12-19 Lidak Pharmaceuticals Human immune system in non-human animal
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
GB8823869D0 (en) 1988-10-12 1988-11-16 Medical Res Council Production of antibodies
US6673986B1 (en) 1990-01-12 2004-01-06 Abgenix, Inc. Generation of xenogeneic antibodies
US6075181A (en) 1990-01-12 2000-06-13 Abgenix, Inc. Human antibodies derived from immunized xenomice
US6150584A (en) 1990-01-12 2000-11-21 Abgenix, Inc. Human antibodies derived from immunized xenomice
EP1690934A3 (en) 1990-01-12 2008-07-30 Abgenix, Inc. Generation of xenogeneic antibodies
US5427908A (en) 1990-05-01 1995-06-27 Affymax Technologies N.V. Recombinant library screening methods
GB9015198D0 (en) 1990-07-10 1990-08-29 Brien Caroline J O Binding substance
US6172197B1 (en) 1991-07-10 2001-01-09 Medical Research Council Methods for producing members of specific binding pairs
US5877397A (en) 1990-08-29 1999-03-02 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US5770429A (en) 1990-08-29 1998-06-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5874299A (en) 1990-08-29 1999-02-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
ATE300615T1 (de) 1990-08-29 2005-08-15 Genpharm Int Transgene mäuse fähig zur produktion heterologer antikörper
US6300129B1 (en) 1990-08-29 2001-10-09 Genpharm International Transgenic non-human animals for producing heterologous antibodies
US6255458B1 (en) 1990-08-29 2001-07-03 Genpharm International High affinity human antibodies and human antibodies against digoxin
US5633425A (en) 1990-08-29 1997-05-27 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US5661016A (en) 1990-08-29 1997-08-26 Genpharm International Inc. Transgenic non-human animals capable of producing heterologous antibodies of various isotypes
US5545806A (en) 1990-08-29 1996-08-13 Genpharm International, Inc. Ransgenic non-human animals for producing heterologous antibodies
US5814318A (en) 1990-08-29 1998-09-29 Genpharm International Inc. Transgenic non-human animals for producing heterologous antibodies
US5789650A (en) 1990-08-29 1998-08-04 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
US5625126A (en) 1990-08-29 1997-04-29 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
ES2341666T3 (es) 1991-12-02 2010-06-24 Medimmune Limited Produccion de autoanticuerpos de repertorios de segmentos de anticue rpos expresados en la superficie de fagos.
CA2124967C (en) 1991-12-17 2008-04-08 Nils Lonberg Transgenic non-human animals capable of producing heterologous antibodies
CA2161351C (en) 1993-04-26 2010-12-21 Nils Lonberg Transgenic non-human animals capable of producing heterologous antibodies
US6013763A (en) * 1996-06-04 2000-01-11 Genentech, Inc. Peptide variants of protein A
IL148079A0 (en) 1999-08-24 2002-09-12 Medarex Inc Human ctla-4 antibodies and compositions containing the same
CN1487996B (zh) 2000-11-30 2010-06-16 米德列斯公司 用于生产人类抗体的转基因转染色体啮齿动物
EP3601362A4 (en) * 2017-03-30 2020-12-16 The Johns Hopkins University HIGH AFFINITY PROTEIN SUPRAMOLECULAR BINDING SYSTEM FOR BIOMACROMOLECULES PURIFICATION
JP7379323B2 (ja) * 2017-08-18 2023-11-14 ザ・ジョンズ・ホプキンス・ユニバーシティ タンパク質精製のための超分子フィラメント集合体

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090214421A1 (en) 2005-05-17 2009-08-27 Sapporo-Medical University ANTIBODY DIRECTED AGAINST PAP2a AND USE THEREOF FOR DIAGNOSTIC AND THERAPEUTIC PURPOSES
US20110201112A1 (en) 2009-11-19 2011-08-18 The Regents Of The University Of California Vault Complexes for Facilitating Biomolecule Delivery

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
D. Missirlis et al, Biochemistry, 2009, 48, 3304-3314 1부.*

Also Published As

Publication number Publication date
CN110741017B (zh) 2023-08-29
EP3601362A4 (en) 2020-12-16
US20260049121A1 (en) 2026-02-19
WO2018183417A1 (en) 2018-10-04
KR20230148855A (ko) 2023-10-25
US20200062827A1 (en) 2020-02-27
CN117024534A (zh) 2023-11-10
US12466875B2 (en) 2025-11-11
JP7229169B2 (ja) 2023-02-27
EP3601362A1 (en) 2020-02-05
KR20190138648A (ko) 2019-12-13
US20230050031A1 (en) 2023-02-16
JP2023011689A (ja) 2023-01-24
CN110741017A (zh) 2020-01-31
US11359005B2 (en) 2022-06-14
JP2020512385A (ja) 2020-04-23

Similar Documents

Publication Publication Date Title
US11292841B2 (en) Anti-PD-1 nano-antibody and application thereof
CN1871256B (zh) 泪脂质运载蛋白的突变蛋白
JP2023011689A (ja) 生体高分子の精製のための超分子高アフィニティタンパク質結合系
CN105722855B (zh) 恒定链经修饰的双特异性五价和六价Ig-M抗体
JP7794789B2 (ja) タンパク質精製のための超分子フィラメント集合体
WO2017157334A1 (zh) 抗pd-l1纳米抗体及其编码序列和用途
WO2024046239A1 (zh) 靶向人gprc5d的重组人源化单克隆抗体及其应用
KR20150023957A (ko) 혈소판-유래 성장인자에 결합하는 설계된 안키린 반복 단백질
JP2016521688A (ja) 肝細胞増殖因子に結合する設計アンキリン反復タンパク質
KR20100044128A (ko) 바이포달 펩타이드 바인더
CN103391787A (zh) uPAR拮抗剂及其应用
WO2022166720A1 (zh) 基于血清白蛋白的融合蛋白、纳米组装体及其制备方法和应用
RU2478645C2 (ru) Растворимый мутант рецептора фактора некроза опухоли
CN109867725A (zh) PD-1-Fc融合蛋白及其制备方法和用途
TW201102086A (en) Antibodies against human CCN1 and uses thereof
CN111378039A (zh) 治疗恶性肿瘤的抗体及其应用
CN110885377A (zh) 抗cd47/vegf双特异性抗体及其应用
CN113912716B (zh) 针对α-突触核蛋白抗原的抗体及其应用
CA3124195A1 (en) Fusion protein with a toxin and scaffold protein
KR101775625B1 (ko) 혈장 내에서 안정성이 증가된 신규한 세포투과성 펩타이드 및 이것의 용도
KR20240038740A (ko) 결합-촉발된 항체 침전 및 정제를 위한 고친화성 초분자 중합체
CN115583995A (zh) 双特异性抗体及其制备方法和应用
TW202506726A (zh) 雙特異性工程化抗體
CN115991785A (zh) 一种双特异性抗体、制备方法和用途
WO2011132940A2 (ko) Rtk에 특이적으로 결합하는 rtk-bpb

Legal Events

Date Code Title Description
PA0105 International application

Patent event date: 20191023

Patent event code: PA01051R01D

Comment text: International Patent Application

PG1501 Laying open of application
A201 Request for examination
PA0201 Request for examination

Patent event code: PA02012R01D

Patent event date: 20210323

Comment text: Request for Examination of Application

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

Comment text: Notification of reason for refusal

Patent event date: 20230309

Patent event code: PE09021S01D

E90F Notification of reason for final refusal
PE0902 Notice of grounds for rejection

Comment text: Final Notice of Reason for Refusal

Patent event date: 20230713

Patent event code: PE09021S02D

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

Patent event code: PE07011S01D

Comment text: Decision to Grant Registration

Patent event date: 20240221

GRNT Written decision to grant
PR0701 Registration of establishment

Comment text: Registration of Establishment

Patent event date: 20240422

Patent event code: PR07011E01D

PR1002 Payment of registration fee

Payment date: 20240422

End annual number: 3

Start annual number: 1

PG1601 Publication of registration