KR102639344B1 - Complex product of ginseng aerial part and soy protein having enhanced anti-oxidant, skin whitening, skin regeneration and/or anti-inflammatory effect, composition comprising the same and method for prepapring the same - Google Patents

Abstract

Disclosed herein are complex processed products of ginseng aerial parts and soy protein, compositions containing the same, and methods for producing the same. The composite processed product includes ginseng aerial parts and soy protein, and the ginseng aerial parts and soy protein are heat-treated together. The composition contains a complex processed product of ginseng aerial parts and soy protein as an active ingredient and has further enhanced antioxidant, whitening, skin regeneration and/or anti-inflammatory activities. The manufacturing method includes mixing ginseng aerial parts and soy protein; And it includes the step of heat-treating the mixed aerial parts of ginseng and soy protein together.

Description

Complex processed product of ginseng aerial parts and soy protein with enhanced antioxidant, whitening, skin regeneration and/or anti-inflammatory activities, composition containing the same, and method for manufacturing the same {COMPLEX PRODUCT OF GINSENG AERIAL PART AND SOY PROTEIN HAVING ENHANCED ANTI-OXIDANT, SKIN WHITENING, SKIN REGENERATION AND/OR ANTI-INFLAMMATORY EFFECT, COMPOSITION COMPRISING THE SAME AND METHOD FOR PREPAPRING THE SAME}

The present disclosure discloses a composite processed product of ginseng aerial parts and soy protein, a composition containing the same, and a method for producing the same.

Ginseng is used as a traditional medicine with various health-promoting functions in Korea and other Asian countries, and is known for its excellent efficacy in anti-cancer, improving diabetes, treating stomach ulcers, promoting brain activity, and preventing aging. Representative ingredients include ginseng saponins called ginsenosides.

Ginsenosides exist in the form of glycosides in fresh and white ginseng, and are reported to have a low absorption rate in the body. The process of deglycosylating these ginsenosides to increase their absorption into the body is the red ginseng process. Desugarization is largely accomplished through physicochemical reactions and biological reactions. Microwave irradiation, vinegar, heat, etc. are used for physicochemical reactions, and enzymes, endophytes, human intestinal bacteria, etc. are used for biological reactions. Existing traditional red ginseng is manufactured using the 9-jeung-9-poly method, which has the disadvantage of consuming a lot of time and money.

Meanwhile, soybeans contain about 37% protein. Considering that the amount used is significantly small compared to the enormous amount of by-products left after manufacturing soybean oil or tofu, there is a need to prevent the waste of food raw materials and develop foods that improve nutritional and sensory values. Soy protein has excellent nutritional value comparable to beef protein, but only about 10% is actually consumed directly by humans, and most of the rest is used as feed.

When defatted soybean flour is processed and turned into food, it has a disgusting smell like soybean odor, which greatly reduces its palatability. To overcome these problems, concentrated soy protein, isolated soy protein, and artificial meat are being developed, but the processing process is complicated, and most soy proteins use organic solvents, so there are stability issues because the solvent remains in the product. Pressed soybean oil and pressed soy protein manufactured by pressing have excellent stability in organic solvents, but are less economical and efficient, so development of uses is necessary. In order to produce pressed soybean oil and use the obtained pressed soybean flakes as soy protein products, a high-pressure protein molding process is required to remove the soybean smell and improve the texture of the protein. This protein molding process has the problem of increasing time and cost. In order to utilize high value-added soybean proteins, including defatted soybean flour, soybean protein flakes, and compressed soybean flakes, it is necessary to develop processed soybean proteins that reduce costs and simplify the process while eliminating soybean smell and residual solvents.

KR 10-1591203 B1

In one aspect, the present disclosure aims to provide a composite processed product of ginseng aerial parts and soy protein.

In another aspect, the purpose of the present disclosure is to provide an antioxidant composition containing the complex processed product of the above-mentioned ginseng aerial parts and soy protein as an active ingredient.

In another aspect, the purpose of the present disclosure is to provide a whitening composition containing the complex processed product of the above-mentioned aerial parts of ginseng and soy protein as an active ingredient.

In another aspect, the purpose of the present disclosure is to provide a composition for skin regeneration comprising a complex processed product of the aerial parts of ginseng and soy protein as an active ingredient.

In another aspect, the purpose of the present disclosure is to provide an anti-inflammatory composition containing the complex processed product of the above-mentioned ginseng aerial parts and soy protein as an active ingredient.

In another aspect, the present disclosure aims to provide a food containing a complex processed product of the above-mentioned ginseng aerial parts and soy protein.

In another aspect, the present disclosure aims to provide a method for producing a composite product of the above-mentioned ginseng aerial parts and soy protein.

In another aspect, the present disclosure aims to provide a method for increasing the antioxidant, whitening, skin regenerative and/or anti-inflammatory efficacy of ginseng aerial parts.

In one aspect, the present disclosure provides a composite processed product of ginseng aerial parts and soy protein, comprising ginseng aerial parts and soy protein.

In one exemplary embodiment, the aerial part of ginseng may be the aerial part of ginseng sprouts.

In an exemplary embodiment, the above-mentioned ginseng aerial parts and soy protein may be heat-treated together.

In an exemplary embodiment, the heat treatment may be performed in an autoclave.

In an exemplary embodiment, the heat treatment may be performed at 110 to 150 °C.

In one exemplary embodiment, the heat treatment may be performed for 10 minutes to 6 hours.

In an exemplary embodiment, the above-mentioned ginseng aerial parts and soy protein may be mixed at a weight ratio of 5 to 15:1.

In another aspect, the present disclosure provides an antioxidant composition comprising a complex processed product of the above-mentioned ginseng aerial parts and soy protein as an active ingredient.

In another aspect, the present disclosure provides a whitening composition comprising a composite processed product of the aerial part of ginseng and soy protein as an active ingredient.

In another aspect, the present disclosure provides a composition for skin regeneration comprising a complex processed product of the aerial parts of ginseng and soy protein as an active ingredient.

In another aspect, the present disclosure provides an anti-inflammatory composition comprising a complex processed product of the aerial parts of ginseng and soy protein as an active ingredient.

In another aspect, the present disclosure provides a food comprising a composite processed product of the above ginseng aerial parts and soy protein.

In another aspect, the present disclosure provides a method for producing a composite product of the above-mentioned ginseng aerial parts and soy protein, comprising the steps of mixing ginseng aerial parts and soy protein; and heat-treating the mixed aerial parts of ginseng and soy protein together.

In one exemplary embodiment, the step of pulverizing the aerial parts of ginseng and soy protein may be further included before the mixing step.

In one aspect, the technology disclosed in the present disclosure has the effect of providing a composite processed product of ginseng aerial parts and soy protein.

In another aspect, the technology disclosed in the present disclosure has the effect of providing an antioxidant composition containing the complex processed product of the above-mentioned ginseng aerial parts and soy protein as an active ingredient.

In another aspect, the technology disclosed in the present disclosure has the effect of providing a whitening composition containing the complex processed product of the aerial part of ginseng and soy protein as an active ingredient.

In another aspect, the technology disclosed in the present disclosure has the effect of providing a composition for skin regeneration containing the complex processed product of the aerial part of ginseng and soy protein as an active ingredient.

In another aspect, the technology disclosed in the present disclosure has the effect of providing an anti-inflammatory composition containing the complex processed product of the aerial part of ginseng and soy protein as an active ingredient.

In another aspect, the technology disclosed in the present disclosure has the effect of providing a food containing a complex processed product of the above-mentioned ginseng aerial parts and soy protein.

In another aspect, the technology disclosed in the present disclosure has the effect of providing a method for producing a composite product of the above-mentioned ginseng aerial parts and soy protein.

In another aspect, the technology disclosed in the present disclosure has the effect of providing a method of increasing the antioxidant, whitening, skin regenerative and/or anti-inflammatory efficacy of ginseng aerial parts.

Figure 1 shows a process flow chart for manufacturing and analyzing a composite product of ginseng aerial parts and soy protein according to an embodiment.
Figure 2 shows HPLC analysis conditions according to an experimental example.
Figure 3 compares the HPLC analysis results of a processed product of ginseng aerial parts and a heat-treated product of ginseng aerial parts alone according to an experimental example.
Figure 4 compares the HPLC analysis results of a single heat-treated product of the aerial part of ginseng and a composite product obtained by heat-treating the aerial part of ginseng together with soy protein according to an experimental example.
Figure 5 shows the HPLC analysis results (734 nm) of processed ginseng aerial parts, single heat-treated ginseng aerial parts, and composite processed products obtained by heat-treating ginseng aerial parts together with soy protein according to an experimental example, comparing peak area values for each active ingredient. It was done.
Figure 6 shows the HPLC analysis results (280 nm) of processed ginseng aerial parts, single heat-treated ginseng aerial parts, and composite processed products obtained by heat-treating ginseng aerial parts together with soy protein according to an experimental example, comparing peak area values for each active ingredient. It was done.
Figure 7 shows the UV spectrum of component No. 1 (λ _max : 284 nm) having antioxidant activity according to an experimental example.
Figure 8 shows the results of comparing the total antioxidant power of processed ginseng aerial parts, singly heat-treated ginseng aerial parts, mixed processed products of ginseng aerial parts and soy protein, and composite processed products obtained by heat-treating ginseng aerial parts together with soy protein according to an experimental example.
Figure 9 shows the results of comparing the tyrosinase activity inhibition ability of processed ginseng aerial parts, singly heat-treated ginseng aerial parts, mixed processed products of ginseng aerial parts and soy protein, and composite processed products obtained by heat-treating ginseng aerial parts together with soy protein according to an experimental example. will be.
Figure 10 shows the results of comparing the cell regeneration capacity of a mixed product of ginseng aerial parts and soy protein and a composite product of heat-treating ginseng aerial parts with soy protein according to an experimental example (WGFSP: mixed processed product of ginseng aerial parts and soy protein, WGFSP_AC : Complex processed product obtained by heat-treating the aerial parts of ginseng with soy protein).
Figure 11 shows the results of cytotoxicity tests on processed products of ginseng aerial parts, single heat-treated products of ginseng aerial parts, mixed products of ginseng aerial parts and soy protein, and composite products obtained by heat-treating ginseng aerial parts together with soy protein according to an experimental example.
Figure 12 shows the results of evaluating the anti-inflammatory efficacy (measurement of nitric oxide production) of a composite product obtained by heat-treating the aerial part of ginseng with soy protein according to an experimental example.

Hereinafter, the present disclosure will be described in detail.

The present disclosure provides a composite processed product that further improves the physiological activity of ginseng aerial parts and soy protein through fusion processing of ginseng aerial parts and soy protein. The complex processed product can provide excellent economic effects by using discarded ginseng by-products and soybean by-products by utilizing the aerial parts of ginseng and soybean protein.

In one aspect, the present disclosure provides a composite processed product of ginseng aerial parts and soy protein, comprising ginseng aerial parts and soy protein.

It is known that the aerial part of ginseng contains a large amount of active ingredients, such as polyphenols and saponins, compared to the ginseng root. In one exemplary embodiment, the aerial part of ginseng may be the aerial part of ginseng sprouts.

In one exemplary embodiment, the above-ground part of ginseng may be the above-ground part of hydroponic ginseng. Unlike conventional soil-cultivated ginseng, which contains large amounts of residual pesticides and heavy metals due to long-term cultivation and is expensive, using hydroponic ginseng grown for about 1 to 5 weeks eliminates the difficulty of removing foreign substances and pesticides and is cost-effective.

In an exemplary embodiment, the ginseng may be dried using a room temperature vacuum drying method.

In this institution, the aerial part of ginseng is dried at room temperature and vacuum, and the water vapor coming out of the dryer is cooled and collected, and the result obtained is called the cell number of the aerial part of ginseng. The cell number of the above-mentioned ginseng aerial part is extracted by evaporating water vapor in a vacuum at room temperature without applying heat, and contains only moisture and volatile components of the aerial part of ginseng.

In an exemplary embodiment, the composite processed product may further include the number of cells in the aerial part of ginseng.

In the present disclosure, soy protein refers to a by-product remaining after removing oil from soybeans, and the milking process can be performed by a person skilled in the art by appropriately selecting a method known in the art. For example, the soy protein may be a compressed soy protein, which is a by-product remaining after oil is removed from soybeans by pressing without using an organic solvent, for example, by pressing at room temperature. Alternatively, it may be a by-product remaining after oil has been removed from soybeans using a vacuum milking method. The soy protein is rich in arginine and glycine and is known to be effective in preventing and treating hypercholesterolemia by lowering the level of insulin in the blood and reducing cholesterol synthesis in the liver. In addition, soy protein is known to have cholesterol-lowering effects, anti-cancer effects, and osteoporosis prevention effects.

In an exemplary embodiment, the soy protein has the shape of a compressed product, a lump, etc., which remains as a by-product after removing the oil from the soybean, or is made into flakes or powder by applying additional processes such as kneading, molding, drying, grinding, and grinding. It may be processed, etc.

In an exemplary embodiment, the soy protein may have a protein content of 35% by weight or more.

In an exemplary embodiment, the soy protein may be soy protein.

In an exemplary embodiment, the soy protein may be defatted soybeans.

In an exemplary embodiment, the soy protein may be pressed soy flakes or defatted soy flour.

The pressed soybean flakes contain about 6% of fat and are low-fat pressed soy protein remaining as a by-product after extracting soybean oil without performing an milking process using an organic solvent.

The defatted soybean powder is a low-fat soy protein obtained by extracting the oil by pressing or removing the oil with a solvent and processing the remaining by-product into powder form.

In an exemplary embodiment, the above-mentioned ginseng aerial parts and soy protein may be heat-treated together. The composite processed product according to the present disclosure provides superior antioxidant, whitening, skin regeneration and/or anti-inflammatory effects by heat-processing the aerial parts of ginseng together with soy protein, and improves skin health compared to a product obtained by heat-processing the aerial parts of ginseng alone. It's more effective.

In an exemplary embodiment, the heat treatment may be performed in an autoclave.

In an exemplary embodiment, the heat treatment may be performed at 110 to 150 °C. In another exemplary embodiment, the heat treatment is above 110°C, above 115°C, above 120°C, above 125°C, above 130°C, above 135°C or above 140°C, below 150°C, below 145°C, below 140°C. Hereinafter, it may be carried out at a temperature of 135 ℃ or less, 130 ℃ or less, 125 ℃ or less, or 120 ℃ or less.

In one exemplary embodiment, the heat treatment may be performed for 10 minutes to 6 hours. In another exemplary embodiment, the heat treatment is 10 minutes or more, 30 minutes or more, 1 hour or more, 2 hours or more, 3 hours or more, 4 hours or more, or 5 hours or more, 6 hours or less, 5 hours or less, 4 hours or less. Hereinafter, it may be carried out for 3 hours or less, 2 hours or less, 1 hour or less, or 30 minutes or less.

In an exemplary embodiment, the above-mentioned ginseng aerial parts and soy protein may be mixed at a weight ratio of 5 to 15:1. In another exemplary embodiment, the above-mentioned ginseng aerial parts and soy protein may be preferably mixed at a weight ratio of 6 to 10:1 or 7 to 9:1 or 8:1.

In an exemplary embodiment, the composite processed product of the above-mentioned ginseng aerial parts and soy protein is processed into the form of flakes, powder, liquid, solid, etc. by applying additional processes such as kneading, molding, drying, grinding, grinding, extraction, and fermentation after heat treatment. It may have happened.

The composite processed product of ginseng aerial parts and soy protein according to the present disclosure has the effect of providing a low-fat, high-protein processed product containing ginseng aerial parts with further enhanced physiological activity. By heat-processing the above-ground parts of ginseng with soy protein, the physiological activity of the above-ground parts of ginseng is further improved. Consumption of animal protein is high in saturated fatty acids and cholesterol, while consumption of vegetable protein such as soy protein is very beneficial as it contains high-quality nutrients such as various vitamins, unsaturated fatty acids, and polyphenols, and has low total calories. In particular, the isoflavones contained in soybeans are effective in preventing cardiovascular disease by lowering blood cholesterol and dilating arteries, and are also effective in improving immunity.

In another aspect, the present disclosure provides an antioxidant composition comprising a complex processed product of the above-mentioned ginseng aerial parts and soy protein as an active ingredient.

In the present disclosure, antioxidant refers to the effect of preventing, inhibiting, inhibiting, improving and/or delaying oxidation processes.

In another aspect, the present disclosure provides a whitening composition comprising a composite processed product of the aerial part of ginseng and soy protein as an active ingredient.

In one exemplary embodiment, the composite processed product may have an increased tyrosinase inhibition ability compared to a processed product obtained by heat-treating the aerial part of ginseng alone.

In another aspect, the present disclosure provides a composition for skin regeneration comprising a complex processed product of the aerial parts of ginseng and soy protein as an active ingredient.

In the present disclosure, skin regeneration refers to the effect of assisting the recovery process of tissue against skin damage. For example, skin regeneration is a biological process that includes chemotaxis, cell differentiation and replication, synthesis of matrix proteins, angiogenesis, and/or wound reorganization.

In another aspect, the present disclosure provides an anti-inflammatory composition comprising a complex processed product of the aerial parts of ginseng and soy protein as an active ingredient.

In the present disclosure, anti-inflammatory refers to the efficacy of preventing, suppressing, inhibiting, improving and/or treating inflammation and/or inflammation-related diseases. Inflammation refers to edema caused by an increase in body fluid between tissue cells and hyperemia caused by vasodilation. It appears as symptoms or signs such as fever due to pyrogenic substances and vasodilation, pain due to arachidonic acid metabolites, etc., and can be classified into acute, subacute, and chronic inflammatory diseases depending on time, and infectious and allergic depending on the pathophysiology. , can be classified into autoimmune, toxic, metabolic, and traumatic inflammatory diseases.

In an exemplary embodiment, the inflammation includes respiratory inflammatory diseases such as rhinitis, sinusitis, otitis media, nasopharyngitis, laryngitis, bronchitis, asthma, chronic obstructive pulmonary disease, bronchiectasis, bronchiolitis, pneumonia, and pulmonary fibrosis; Inflammatory diseases of the digestive system such as stomatitis, esophagitis, gastritis, peptic ulcer, irritable bowel syndrome, inflammatory bowel disease, cholecystitis, cholangitis, pancreatitis, and hepatitis; Skin inflammatory diseases such as acne, contact dermatitis, seborrheic dermatitis, atopic dermatitis, and psoriasis; Cardiovascular inflammatory diseases such as endocarditis, myocarditis, pericarditis, vasculitis, arteriosclerosis, and sepsis; Endocrine inflammatory diseases such as thyroiditis, parathyroidism, and diabetes; Urogenital inflammatory diseases such as glomerulonephritis, nephropathy, interstitial kidney disease, orchitis, oophoritis, endometritis, and vaginitis; Musculoskeletal inflammatory diseases such as rheumatoid arthritis, spondyloarthropathy, osteoarthritis, gout, systemic lupus erythematosus, systemic sclerosis, myopathy, Sjögren's syndrome, Behcet's disease, and antiphospholipid syndrome; It may be selected from the group consisting of neuropsychiatric inflammatory diseases such as vascular dementia, Alzheimer's disease, degenerative brain disease, depression, and schizophrenia.

In the present disclosure, an active ingredient refers to an ingredient that can exhibit the desired activity alone or in combination with a carrier that is inactive on its own.

In one exemplary embodiment, the composition may be a food composition.

In an exemplary embodiment, the composition may be a cosmetic composition.

The composition contains a composite processed product of ginseng aerial parts and soy protein as an active ingredient, and has the effect of providing better antioxidant, whitening, skin regeneration and/or anti-inflammatory activities compared to a product obtained by heat-treating the aerial parts of ginseng alone.

In an exemplary embodiment, the cosmetic composition can be applied to atopic dermatitis improvement cosmetics, anti-aging cosmetics, skin regeneration promotion cosmetics, wound healing cosmetics, whitening cosmetics, moisturizing cosmetics, etc. by utilizing the efficacy of ginseng aerial parts and soy protein.

In another aspect, the present disclosure provides a food comprising a composite processed product of the above-mentioned ginseng aerial parts and soy protein. For example, various foods such as vegan food, protein supplement food, convenience food, sports or energy food, diet food, snack food, frozen food, food for children's growth, food for test takers, and muscle strengthening food for the elderly can be manufactured.

In an exemplary embodiment, the food may be one or more selected from the group consisting of snacks, cereals, noodles, bread, snacks, ice cream, frozen desserts, soybean meat, beverages, candy, chocolate, and jelly.

In an exemplary embodiment, the food may be a health functional food.

In another aspect, the present disclosure provides a method for producing a composite product of the above-mentioned ginseng aerial parts and soy protein, comprising the steps of mixing ginseng aerial parts and soy protein; and heat-treating the mixed aerial parts of ginseng and soy protein together.

In another aspect, the technology disclosed in this disclosure includes mixing ginseng aerial parts and soy protein; And it provides a method of increasing the antioxidant, whitening, skin regenerative and/or anti-inflammatory effects of ginseng aerial parts, comprising the step of heat-treating the mixed aerial parts of ginseng and soy protein. Here, 'increase' refers to the antioxidant, whitening, skin regeneration and/or anti-inflammatory effects of the ginseng aerial parts before heat treatment with soy protein, the antioxidant, whitening, and anti-inflammatory effects of the ginseng aerial parts heat-treated alone without mixing with soy protein. It may be increased compared to the skin regeneration and/or anti-inflammatory effect, or the antioxidant, whitening, skin regeneration and/or anti-inflammatory effect of a simple mixture of ginseng aerial parts and soy protein.

In one exemplary embodiment, the step of pulverizing the aerial parts of ginseng and soy protein may be further included before the mixing step.

The method for producing a complex product of ginseng aerial parts and soy protein according to the present disclosure not only further improves the functionality of ginseng aerial parts, but also removes soybean smell and improves the texture of the protein to produce a soy protein product. It has the effect of further simplifying the molding process and significantly reducing costs.

Hereinafter, the present disclosure will be described in more detail through examples. It will be apparent to those skilled in the art that these examples are solely for illustrating the present disclosure, and that the scope of the present disclosure should not be construed as limited by these examples.

Example. Composite workpiece manufacturing

The aerial parts of frozen ginseng sprouts were crushed to obtain ground ginseng aerial parts. In addition, crushed soy protein was obtained by grinding pressed soy protein. Afterwards, 2 g of ground ginseng aerial parts and 0.25 g of ground soy protein were mixed (weight ratio of 8:1), and this mixture was heat-treated at 121°C for 3 hours under an autoclave. Accordingly, a composite processed product of ground ginseng parts and soy protein mixed and then heat-treated was obtained (see Figure 1).

Comparative example. Workpiece manufacturing

First, the aerial parts of frozen ginseng sprouts were pulverized to obtain processed products of the pulverized aerial parts of ginseng.

In addition, 2 g of the pulverized aerial part of ginseng was heat-treated at 120° C. for 3 hours in an autoclave to obtain a heat-treated aerial part of ginseng.

In addition, the above-ground part of the frozen ginseng sprout was crushed and the pressed soy protein was crushed, and then 2 g of the ground part of the ginseng sprout and 0.25 g of the ground soy protein were mixed (weight ratio of 8:1) to mix the above-ground part of the ginseng with the above-ground part of the ginseng. A mixed processed product of soy protein was obtained.

Experimental Example 1. Extract preparation

In order to comparatively analyze the processed products prepared in the above Examples and Comparative Examples, extracts were prepared as follows.

100 mL of 70% (v/v) ethanol aqueous solution was added to 1 g of each processed material, and then extracted by sonicating for 1 hour. Afterwards, it was gravity filtered with filter paper (Whatman No. 1), and part of the filtrate was used for component analysis and antioxidant efficacy evaluation, and the remaining filtrate was concentrated under reduced pressure and used for experiments evaluating whitening, skin regeneration, and anti-inflammatory effects (Figure 1).

Extract weight (mg) Extraction Yield Ginseng above ground part 60.5 0.03025 Exclusive heat processing of the above-ground part of ginseng 42.1 0.02105 Ginseng aerial parts-soy protein mixture 91.9 0.04595 Ginseng aerial part-soy protein heat-treated product 107.4 0.0537

Extraction yield = weight of raw material/extract

Experimental Example 2. Ingredient analysis

To analyze the bioactive components of each processed product manufactured in the above Examples and Comparative Examples, HPLC analysis was performed as follows.

Each extract was diluted to a concentration of 10 mg/ml using 70% (v/v) ethanol aqueous solution, dissolved by ultrasound, and then filtered through a 0.45 μm PVDF syringe filter (Whatman, Maidstone, England). The filtered extract was analyzed using an HPLC device (1200 series, Agilent Technologies). Active ingredients with ABTS radical scavenging ability were identified using Online HPLC-ABTS. The ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) reaction reagent is prepared as a 2mM ABTS solution containing 3.5mM potassium persulfate and stored for 12 hours at room temperature in the dark before use. It has been done. 734 nm was used as an appropriate wavelength to determine the scavenging ability of free radicals. The column is YMC Pack-ODS A C18 column (4.6 and water (B) <0 min 10% A; 0-30 minutes 10-100% A; 30-35 minutes 100% A; 35-37 minutes 100-10% A; A linear gradient was set at 10% A for 37-40 minutes, the flow rate was 0.7 ml/min, and the sample injection volume was 10 μL (see Figure 2). The bioactive component profile of the processed product was represented by a chromatogram at 280 nm.

As a result, as shown in Figures 3 to 7, the area value of components 1 to 7 at 734 nm was found to increase in the individually heat-treated processed product of the aerial part of ginseng compared to before heat treatment processing. Accordingly, it was confirmed that antioxidant components were produced. In addition, the composite processed product obtained by heat-treating the aerial part of ginseng with soy protein showed an increase in the area value of component 1 at 734 nm and a decrease in the area value of components 2 to 7 compared to the single heat-treated product of the aerial part of ginseng. When the above-ground part of ginseng was heat-treated with soy protein, the content of ingredient No. 1, which had the highest antioxidant activity at 734 nm, was confirmed to significantly increase.

Experiment example 3. Antioxidant efficacy evaluation

The DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging activity of each processed product prepared in the above Examples and Comparative Examples was measured to compare the antioxidant efficacy.

All dilution solvents used were 70% (v/v) ethanol. The DPPH reagent was used from Santa Cruz, and the DPPH reagent (molecular weight: 394.32 g/mol) was prepared at a concentration of 0.1mM and used in the experiment. Samples were prepared at concentrations of 1, 0.5, 0.25, and 0.125 mg/mL, and Vitamin C was used as a positive control at a concentration of 50 ppm to 0.390625 ppm by adjusting the concentration depending on the DPPH radical scavenging ability of the sample. The control group (Blank) used DPPH solution and 70% (v/v) ethanol. DPPH solution was added and reacted for 30 minutes, and then measured at a wavelength of 517 nm. The DPPH radical scavenging ability was calculated as a percentage according to the formula below, and the total antioxidant power was evaluated according to the formula below using the extraction number in Table 1 above. It is shown in 8.

DPPH radical scavenging ability (%) = [1-(Sample O.D / Control O.D.)]×100

Total antioxidant power = DPPH radical scavenging power at each concentration (%) × extraction yield

As a result, it was confirmed that the total antioxidant power of the mixed heat-treated product of ginseng aerial parts and soy protein was significantly higher than that of the heat-treated product of ginseng aerial parts alone or the mixed product of ginseng aerial parts and soy protein.

Experimental Example 4. Whitening efficacy evaluation

The skin whitening efficacy was compared by measuring the tyrosinase activity inhibition effect ( in vitro ) of each processed product prepared in the above Examples and Comparative Examples.

The efficacy of the test sample in inhibiting tyrosinase activity was evaluated by reacting the test sample, purified tyrosinase, and the substrate tyrosine in vitro. Specifically, the sample was dissolved in 70% (v/v) ethanol, diluted to set a concentration range that can confirm inhibition of tyrosinase activity, and treated to reach at least 5 concentrations. Then, it was added to a test tube with 0.1 M phosphate buffer (pH 6.5). ) Add 220 μL of sample solution, 20 μL of sample solution, and 20 μL of mushroom tyrosinase solution (1500 U/mL to 2000 U/mL) (or human tyrosinase) in that order, then add 40 μL of 1.5 mM tyrosinase solution to this solution. was added and reacted at 37°C for 10 to 15 minutes, then the absorbance was measured at 490 nm, and the results are shown in Figure 9.

Tyrosinase activity inhibition rate (%) = {100 - (b-b'/a-a')} × 100

a: Absorbance after reaction of blank sample solution

b: Absorbance after reaction of sample solution

a', b': Absorbance measured by replacing tyrosinase with buffer solution

As a result, it was confirmed that the inhibitory effect on tyrosinase activity of the mixed heat-treated product of ginseng aerial parts and soy protein was higher than that of the heat-treated product of ginseng aerial parts alone or the mixed product of ginseng aerial parts and soy protein. Accordingly, it was confirmed that a composite product obtained by heat-treating the aerial part of ginseng with soy protein can more effectively inhibit melanin synthesis by inhibiting the tyrosinase enzyme, making it very useful for skin whitening.

Experiment example 5. Evaluation of skin regeneration efficacy

The skin regeneration efficacy was compared by measuring the effect of promoting skin cell migration and proliferation of the composite processed product prepared by heat-treating the aerial part of ginseng and soy protein prepared in the above example and the processed product prepared by mixing the aerial part of ginseng and soy protein prepared in the comparative example.

Keratinocytes (HaCaT cells) used in the experiment were cultured at 37°C and 5% CO ₂ using DMEM medium (Gibco, Grand Island, NY, USA) containing 10% FBS.

In order to protect normal cells from oxidative stress caused by hydrogen peroxide (H ₂ O ₂ ) and to determine whether there is an effect on skin cell proliferation, HaCaT cells were seeded in a 6-well plate using a skin wound cell model (wound healing assay). After aliquoting to a concentration of 1×10 ⁶ cells/mL, the cells were cultured in an incubator at 37°C and 5% CO ₂ until the cells had proliferated by more than 90%. When the cells were sufficiently stabilized, wounding was induced by scraping the culture dish with a sterilized 200 μL pipette tip. After treating each sample at a concentration of 500 μg/mL, the degree of cell movement over time was observed using a microscope. The results are shown in Figure 10.

As a result, compared to the group treated with H ₂ O ₂ alone, it was confirmed that cell growth and cell migration increased in the group treated with a mixed product of ginseng aerial parts and soy protein and in the group treated with a composite product in which ginseng aerial parts were heat-treated together with soy protein. After 72 hours, the growth and migration area of HaCaT cells increased by 6% and 19%, respectively, compared to the group treated with H ₂ O ₂ alone. The cell proliferation effect can be further enhanced by heat-treating the aerial parts of ginseng with soy protein. It was confirmed that it exists. Accordingly, it was confirmed that the composite processed product obtained by heat-treating the aerial parts of ginseng with soy protein induces the proliferation of skin cells by promoting skin cell growth and migration, and has an excellent effect on skin regeneration.

Experiment example 6. Evaluation of anti-inflammatory efficacy

(1) Confirmation of cytotoxicity

RAW 264.7 cells, the macrophage cell line used in the experiment, were purchased from the Korea Cell Line Bank (KTCC, Seoul, Korea). RAW 264.7 cells were incubated in DMEM (Dulbecco's modified Eagle's medium) containing 10% FBS (Fetal bovine serum) with 100 U/ml penicillin and 100 μg/ml streptomycin in a 37°C, 5% CO ₂ incubator. It was cultured in .

Cytotoxicity was measured using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra zolium bromide, Duchefa Biochemie, Haarlem, Netherland) method. RAW 264.7 cells were inoculated into a 96 well plate and cultured for 24 hours at 5% CO ₂ and 37°C. Then, each sample was inoculated at each concentration and cultured for 24 hours. Afterwards, 10 μl of 5 mg/ml MTT reagent was added to each well and left in an incubator at 5% CO ₂ and 37°C for 4 hours. After completely removing the MTT solution and drying the culture plate, formazan crystals formed within the cells were dissolved with 100 μL dimethyl sulfoxide (DMSO, Sigma-Aldrich, USA), and the absorbance was measured at 570 nm with an ELISA plate reader. Cell survival rate was calculated as the ratio of the untreated group and the treated sample group.

As a result, as shown in Figure 11, it was confirmed that none of the samples showed significant cytotoxicity at the treatment concentration of 62.5 to 500 μg/mL.

(2) Inhibition of Nitric Oxide (NO) production

The anti-inflammatory effect of the composite product manufactured in the above example was confirmed through the effect of inhibiting nitric oxide (NO) production.

The RAW 264.7 cells were inoculated in a 96 well plate and cultured at 5% CO ₂ at 37°C for 24 hours, then treated with new medium containing each sample and LPS (1 μg/mL) at the same time and cultured for 24 hours. . The amount of NO produced was 96 μL by mixing 100 μL of cell culture supernatant with 100 μL of Griess reagent [1 % (w/v) sulfanilamide, 0.1 % (w/v) naphtyl-ethylenediamine in 2.5 % (v/v) phosphoric acid]. After reacting for 10 minutes in a well plate, absorbance was measured at 540 nm using an ELISA microplate reader. The concentration of nitrite produced was calculated using a standard curve of sodium nitrite dissolved in DMEM medium, and the inhibition of NO production in each sample was based on the difference in the amount of nitrite produced in the test group treated with LPS and the control group not treated with LPS. Activity was confirmed. In addition, it was compared with N-monomethyl-L-arginine (L-NMMA), a structural analog of L-arginine, known as a substance that inhibits NO production.

As a result, NO was not produced in the untreated group, whereas NO was produced in the experimental group treated only with LPS, and the amount of NO secreted in the LPS only treated group was converted to 100% to confirm the NO production inhibition effect of the sample. . The composite product obtained by heat-treating the aerial parts of ginseng with soy protein inhibited NO production in a concentration-dependent manner and was confirmed to have a significantly higher NO production inhibition ability than the positive control L-NMMA (see Figure 12). In addition, as confirmed in the cytotoxicity test, the composite product showed no cytotoxicity, indicating that the anti-inflammatory effect was not caused by the cytotoxicity of the composite product.

As described above, specific parts of the present disclosure have been described in detail, and it is clear to those skilled in the art that these specific techniques are merely preferred embodiments and do not limit the scope of the present disclosure. something to do. Accordingly, the substantial scope of the present disclosure will be defined by the appended claims and their equivalents.

Claims (14)

It contains as an active ingredient a complex processed product obtained by heat-treating ground ginseng aerial parts and ground soy protein together,
The heat treatment is carried out at 110 to 150 ° C in an autoclave,
The composite processed product is a cosmetic composition for antioxidant or whitening that improves the antioxidant or whitening effect of the aerial part of ginseng.
According to clause 1,
The above-mentioned ginseng part is the above-ground part of ginseng sprout, a cosmetic composition for antioxidant or whitening.
According to clause 1,
A cosmetic composition for antioxidant or whitening, wherein the heat treatment is performed for 10 minutes to 6 hours.
According to clause 1,
A cosmetic composition for antioxidant or whitening, wherein the pulverized ginseng aerial parts and the pulverized soy protein are mixed at a weight ratio of 5 to 15:1.
According to clause 4,
A cosmetic composition for antioxidant or whitening, wherein the pulverized ginseng aerial parts and pulverized soy protein are mixed at a weight ratio of 7 to 9:1.
It contains as an active ingredient a complex processed product obtained by heat-treating ground ginseng aerial parts and ground soy protein together,
The heat treatment is carried out at 110 to 150 ° C in an autoclave,
The composite processed product is a food composition for antioxidant or whitening that improves the antioxidant or whitening effect of the aerial part of ginseng.
According to clause 6,
The above-mentioned ginseng aerial part is the aerial part of ginseng sprouts, and is a food composition for antioxidant or whitening purposes.
According to clause 6,
A food composition for antioxidant or whitening, wherein the heat treatment is performed for 10 minutes to 6 hours.
According to clause 6,
A food composition for antioxidant or whitening, wherein the pulverized ginseng aerial parts and the pulverized soy protein are mixed at a weight ratio of 5 to 15:1.
According to clause 9,
A food composition for antioxidant or whitening, wherein the pulverized ginseng aerial parts and the pulverized soy protein are mixed at a weight ratio of 7 to 9:1.
Mixing ground ginseng aerial parts and ground soy protein; and
Comprising the step of heat-treating the mixed ginseng aerial part pulverization and soy protein pulverization together to produce a composite processed product,
The heat treatment is carried out at 110 to 150 ° C in an autoclave,
A method of improving the antioxidant or whitening effect of the aerial part of ginseng, wherein the composite processed product enhances the antioxidant or whitening effect of the aerial part of ginseng.
According to clause 11,
A method of enhancing the antioxidant or whitening effect of the above-ground part of ginseng, which is the above-ground part of ginseng sprouts.
According to clause 11,
A method of improving the antioxidant or whitening effect of the aerial part of ginseng, wherein the heat treatment is performed for 10 minutes to 6 hours.
According to clause 11,
A method for enhancing the antioxidant or whitening effect of the aerial parts of ginseng, wherein the pulverized aerial parts of ginseng and the pulverized soy protein are mixed at a weight ratio of 5 to 15:1.