KR102615812B1 - Composition for reducing irritation in skin - Google Patents

Abstract

The present invention relates to a composition having the effect of alleviating skin irritation, and more specifically, to alleviating skin irritation comprising extracts of artichoke leaf, Chistus monspeliensis, galanga root, soap plant and Larea mexicana as active ingredients, It relates to a composition having skin barrier strengthening, anti-allergic effect, and anti-inflammatory activity.

Description

Composition for relieving skin irritation {COMPOSITION FOR REDUCING IRRITATION IN SKIN}

The present invention relates to a composition having the effect of alleviating skin irritation, and more specifically, to alleviating skin irritation comprising extracts of artichoke leaf, Chistus monspeliensis, galanga root, soap plant and Larea mexicana as active ingredients, It relates to a composition having skin barrier strengthening, anti-allergic effect, and anti-inflammatory activity.

Skin is an epithelial tissue that covers and protects the muscles and organs of our body. It protects the human body from the external environment such as ultraviolet rays, heat, wind, pollution, stress, etc., and maintains moisture in the body to regulate body temperature. do. In particular, the epidermal tissue located on the outermost layer of the skin contains a stratum corneum containing Natural Moisturizing Factor (NMF), which helps retain moisture in the skin and strengthens the skin barrier.

However, these days, artificial temperature changes due to changes in the environment and lifestyle patterns, various stresses occurring in social or daily life, and physical stimulation due to continuous wearing of masks induce a continuous inflammatory response and damage the skin barrier function. causes.

As mentioned above, continuous external stimulation causes the secretion of inflammatory cytokines such as TNF (Tumor Necrosis Factor), IL-6 (Interleutin 6), and IL-8 (Interleutin 8), and the secretion of these cytokines If it continues for a long period of time, it can lead to results such as chronic inflammation or epidermalization.

Among commonly used cosmetic ingredients, functional raw materials used for the purpose of removing dead skin cells, improving and preventing wrinkles, etc. are effective, but have the disadvantage of causing side effects such as irritating the skin and causing problems. Therefore, there is a need for the development of substances or compositions effective in alleviating skin irritation and reducing inflammation that reduce skin irritation and inflammation caused by various irritating factors and at the same time minimize side effects.

Against this background, the present inventors have conducted various studies on compositions for relieving skin irritation based on various plant extracts, and the above invention has as its main ingredient a complex extract based on natural ingredients that shows the efficacy of relieving skin irritation and reducing inflammation. was completed.

The purpose of the present invention is to provide a composition and a method for producing the same that relieve skin irritation, strengthen the skin barrier, have anti-allergic effects, and anti-inflammatory activity by reducing the secretion of inflammatory cytokines.

Another object of the present invention is to provide a pharmaceutical composition for the treatment and prevention of inflammatory diseases, which reduces the secretion of inflammatory cytokines.

In order to achieve the above object, the present invention includes artichoke ( Cynara Scolymus ) leaves, Cistus Monspeliensis ( Cistus Monspeliensis ), Galanga ( Kaempferia Galanga ) roots, soap grass ( Saponaria Officinalis ) and Larrea Mexicana Provided is a cosmetic composition comprising an extract of as an active ingredient.

The term “raw material” used in the present invention refers to a raw material before extraction.

The term “extract” as used in the present invention refers to the result obtained by extracting a herbal medicine with an appropriate extraction solvent, including the extract obtained through the extraction treatment process, the diluted or concentrated liquid of the extract, the dried product obtained by drying the extract, Alternatively, it can be interpreted as including purified products or fractions that can be obtained by purifying or fractionating them.

Preferably, it may be a mixture of artichoke leaves, Chistus monspeliensis, galanga root, soap grass and Larea mexicana, more preferably it may be a mixture of artichoke leaves, chestus monspeliensis, galanga root, soap grass and By mixing and extracting the dried material of Larea mexicana, the active ingredient of the composition according to the present invention can be obtained.

In the cosmetic composition, the raw materials of the composition, such as artichoke leaves, Chistus monspeliensis, Galanga root, soap plant, and Larea mexicana, can be used without limitation, whether cultivated, collected, or commercially available. .

The “artichoke ( Cynara Scolymus ) leaf” is a perennial flower belonging to the Asteraceae family. It is native to the Mediterranean coast and is distributed in Korea, Italy, southern France, the United States, and China. It is said to have been cultivated and used for food since ancient Greece and Rome, and in the East, it has been cultivated in China since the early 1900s. In Korea, cultivation has been carried out mainly in Jeju since the early 2000s.

The “ Cistus Monspeliensis ” is an evergreen broad-leaved shrub that grows in areas such as southern Europe and the Mediterranean region of northern Africa, where the climate is mild and dry. In ancient Egypt, its strong scent was used as a raw material for perfume. In the present invention, the usable parts of “Chistus monspeliensis” are not limited.

The “Galanga ( Kaempferia Galanga ) root” is a perennial plant of the ginger family and grows well in tropical climates, so it is mainly distributed in tropical Asia. Traditionally, it has been used as a treatment for respiratory diseases such as fever, cold, cough, sore throat, and asthma, and digestive diseases such as indigestion, abdominal pain, and diarrhea.

The above-mentioned “Soapweed ( Saponaria Officinalis )” is a perennial plant of the genus Soapweed, and is mainly distributed in Europe, Asia, including Korea. It is a plant that grows well in environments with a lot of moisture, and in Korea it is mainly found growing near watersides or rivers. It contains a large amount of saponin, a natural surfactant, and has long been used as a substitute for soap. In the present invention, the areas where the “soap paste” can be used are not limited.

The “Larrea Mexicana ” is a dicotyledonous plant, also known as “Larrea tridentata” and “Creosote bush,” and is mainly distributed in desert areas of North and South America. Native Americans have long used it to relieve pain and heal wounds.

The extract according to the present invention can be prepared using general extraction methods known in the art, for example, boiling water extraction, hot water extraction, cold needle extraction, reflux cooling extraction, ultrasonic extraction, and hot pressure extraction. there is.

Extraction solvents used to prepare the extract according to the present invention include water; lower alcohols having 1 to 4 carbon atoms such as methanol, ethanol, propyl alcohol, and butyl alcohol; One or two or more types selected from polyhydric alcohols such as glycerol, propanediol butylene glycol, propylene glycol, and 1,2-hexanediol may be used, but are not limited thereto.

Preferably, water can be used as the extraction solvent, particularly preferably purified water. The extraction solvent may be used in an amount of 5 to 20 times the dry weight of the composite.

The extract according to the present invention may include not only extracts using the above-described extraction solvents, but also extracts that have undergone conventional purification, enzyme treatment, and fermentation processes. For example, extracts obtained through various purification and extraction methods that can be additionally performed, such as decompression with carbon dioxide, extraction using high temperature or ultrasonic waves, extracts from natural conditions or fermentation products using various microorganisms, etc. may also be included in the extract of the present invention. there is.

The method for producing the composition according to the present invention includes artichoke ( Cynara Scolymus ) leaves, Cistus Monspeliensis ( Cistus Monspeliensis ), Galanga ( Kaempferia Galanga ) roots, Saponaria Officinalis and Larrea Mexicana . A first step of preparing a mixture by mixing, a second step of preparing an extract by adding a solvent and a hydrolytic enzyme to the mixture of the first step, and a third step of filtering the extract of the second step through a filter to obtain a composition; may include.

In the present invention, the three stages of filtration may use, for example, a 0.5 to 1.0 μm filter.

Specifically, in the method for producing the composition according to the present invention, the complex extract is prepared by extracting artichoke leaves, Chistus monspeliensis, Galanga root, soap grass and Larea mexicana with water and hydrolytic enzymes. obtained. For example, artichoke leaves, Chistus monspeliensis, galanga root, soap plant and Larea mexicana can be extracted using distilled water at a temperature of 40°C to 60°C for 6 to 10 hours.

Optionally, it may include washing and drying raw artichoke leaves, Chistus monspeliensis, galanga root, soap plant and Larea mexicana.

Optionally, it may include the step of concentrating under reduced pressure.

Optionally, it may include fractionating the filtrate or concentrate.

Optionally, it may include obtaining fractions through open column chromatography.

According to one embodiment of the present invention, a cosmetic composition comprising extracts of artichoke leaves, Chistus monspeliensis, Galanga root, soap plant and Larea mexicana as active ingredients is provided.

The content of the extract included as an active ingredient in the cosmetic composition according to the present invention can be appropriately determined by considering various factors such as purpose of use, period of use, type of formulation, route of administration, and skin condition of the subject. For example, the content of the complex extract may be 0.001 to 10% by weight, for example, 0.01 to 5% by weight, based on the total weight of the cosmetic composition.

Specifically, the artichoke leaves, Chistus monspeliensis, galanga root, soap grass and Larea mexicana may be mixed in a weight ratio of 5 to 15: 5 to 15: 1 to 10: 1 to 3: 0.5 to 1.5. and preferably mixed at a weight ratio of 10.1:10:1:4.6:2:1.

The composition according to the present invention may inhibit the secretion of inflammatory cytokines. Additionally, the composition according to the present invention may relieve skin irritation.

The composition according to the present invention can be manufactured in the form of a general emulsified formulation, solubilized formulation, etc., using commonly known production methods. At this time, the cosmetic composition of the present invention includes patches, ointments, skin adhesive gels, creams, packs, lotions, essences, sprays, masks, foundations, makeup bases, cleansers, water (W) type, Oil (O) type, silicone (S) type, oil in water (O/W) type, water in oil (W/O) type, water in silicon (W/S) type, silicon in water (S/W) type, solid phase, It can be manufactured in various formulations such as liquid, and commonly used cosmetic manufacturing methods can be applied.

The cosmetic composition according to the present invention may further include additional ingredients in addition to the extract to improve efficacy. For example, the additional ingredients are not limited as long as they do not offset or reduce the effectiveness of the extract. Optionally, auxiliaries, carriers, etc. commonly used in the cosmetics field may be additionally included. Optionally, ingredients typically used to add or enhance cosmetic functions may also be added. For example, stabilizers, emulsifiers, thickeners, moisturizers, liquid crystal film strengtheners, pH adjusters, antibacterial agents, water-soluble polymers, coating agents, metal ion sequestrants, amino acids, organic amines, polymer emulsions, pH adjusters, skin nutrients, antioxidants, One or more water-based additives selected from antioxidants, preservatives, fragrances, etc.; and one or more oil-based additives selected from fats and oils, waxes, hydrocarbon oils, higher fatty acid oils, higher alcohols, synthetic ester oils, and silicone oils.

According to another embodiment of the present invention, there is provided a pharmaceutical composition comprising extracts of artichoke leaves, Chistus monspeliensis, Galanga root, soap plant and Larea mexicana as active ingredients.

As described above, the extracts of artichoke leaves, Chistus monspeliensis, Galanga root, soap grass and Larea mexicana according to the present invention can show efficacy in relieving skin irritation and reducing inflammation. Therefore, the pharmaceutical composition of the present invention containing the extract can be applied for the treatment or prevention of inflammatory diseases.

As used herein, the term “inflammatory disease” refers to a disease that can appear on irritated skin due to environmental changes occurring outside the skin, physical stimulation, stress, etc., or inflammation that can occur due to the above-mentioned irritation or allergy. refers to a disease related to Specifically, the inflammatory disease includes seborrheic dermatitis, contact dermatitis, pruritus, atopic dermatitis, allergic dermatitis, and psoriasis.

The content of the active ingredient contained in the pharmaceutical composition according to the present invention can be appropriately adjusted depending on the symptoms of the disease, the degree of progression of the symptoms, the patient's condition, age, gender, etc. For example, artichoke leaves, Chistus monspeliensis, galanga root, soap plant and Larea mexicana may each be in an amount of 0.1 to 50.0% by weight based on the total weight of the complex extract.

The pharmaceutical composition according to the present invention may further include appropriate carriers, excipients, and diluents commonly used in the manufacture of pharmaceuticals. Carriers, excipients and diluents that may be contained in the composition include, for example, lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginates, gelatin, calcium phosphate, calcium silicate, cellulose. , methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, or mineral oil.

The pharmaceutical composition according to the present invention may be in an oral dosage form. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations are made by adding at least one excipient to the extract, such as starch, calcium carbonate, sucrose or lactose, gelatin, etc. It can be prepared by mixing. Liquid preparations for oral use include suspensions, oral solutions, emulsions, syrups, etc. In addition to the commonly used simple diluents such as water and liquid paraffin, various excipients such as wetting agents, sweeteners, fragrances, and preservatives may be included. . In addition, the pharmaceutical composition according to the present invention can be formulated into a dosage form suitable for topical administration using techniques well known to those skilled in the art, and the dosage form may include an external preparation, an effervescent tablet, a suppository, etc. In one embodiment, the pharmaceutical composition of the present invention can be formulated as an external agent by mixing the extract with a base that is well known and commonly used in the art. The external preparation may be an emulsion, gel, ointment, cream, patch, liniment, powder, aerosol, spray, lotion, serum, paste, foam, drops, suspension, or tincture.

The pharmaceutical composition according to the present invention can be administered in combination with other therapeutic agents, and can also be administered sequentially or individually. For example, the pharmaceutical composition and other therapeutic agents may be administered simultaneously as one preparation, or may be administered simultaneously, sequentially, or separately as separate preparations. At the same time, in order to administer sequentially or individually, the extract and other therapeutic agents included in the pharmaceutical composition of the present invention may be separately formulated in separate containers or may be formulated together in the same container. In addition, the extract and other therapeutic agents included in the pharmaceutical composition of the present invention may have the same or different pharmaceutically effective dose, administration time, administration route, treatment period, etc.

As used in the present invention, “pharmaceutically effective amount” means an amount sufficient to treat a disease with a reasonable benefit/risk ratio applicable to medical treatment, and the effective dose level is determined by the type and severity of the individual, age, gender, and activity of the drug. , can be determined based on factors including sensitivity to the drug, time of administration, route of administration and excretion rate, drugs used simultaneously, and other factors well known in the field of medicine.

The present invention will be described in more detail based on the following examples and drawings attached to this specification, but this is not intended to limit the scope of the present invention. In addition, those skilled in the art will be able to make various changes and modifications to the present invention without impairing the spirit of the present invention.

The composition according to the present invention can effectively relieve skin irritation that may occur from various external environmental changes by suppressing the secretion of inflammatory cytokines.

Since the complex extract included as an active ingredient in the composition according to the present invention is not toxic to skin cells, safety for the human body can be guaranteed.

The composition of the present invention can be applied to various fields that require relief from skin irritation and reduction of inflammation.

Figure 1 shows the human body when treated with single extracts of artichoke leaves, Chistus monspeliensis, galanga root, soap plant and Larea mexicana, plant extracts with different types of plants in the extracts, and complex extracts of the present invention. This is a graph showing the survival rate of keratinocytes.
Figure 2 shows the human body when treated with single extracts of artichoke leaves, Chistus monspeliensis, galanga root, soap plant and Larea mexicana, plant extracts with different types of plants in the extracts, and complex extracts of the present invention. This graph shows the concentration of the cytokine IL-6 secreted from keratinocytes.
Figure 3 shows the human body when treated with single extracts of artichoke leaves, Chistus monspeliensis, galanga root, soap plant and Larea mexicana, plant extracts mixed with different types of plants in the extracts, and complex extracts of the present invention. This is a graph showing the concentration of IL-8, a cytokine secreted from keratinocytes.

Hereinafter, the present invention will be described with specific examples to aid understanding. However, the following examples are intended to illustrate the present invention in more detail, and these examples should not be construed as limiting the present invention, and ordinary changes by those skilled in the art are possible within the scope of the present invention.

In addition, terms, techniques, etc. described in this specification should be understood as commonly used in the technical field to which the present invention pertains, unless there are special limitations.

Example 1. Preparation of complex extract of the present invention

Artichoke ( Cynara Scolymus ) leaves, Cistus Monspeliensis ( Cistus Monspeliensis ), Galanga ( Kaempferia Galanga ) roots, soap plant ( Saponaria Officinalis ), and Larrea Mexicana ( Larrea Mexicana ) were purchased and used in this experiment. 100 g (total weight) of the raw material containing artichoke leaves, Chistus monspeliensis, galanga root, soap plant and Larea mexicana was prepared in the following composition ratio: artichoke leaves 36.4 g (36.4 wt%), chia Stus monspeliensis 36.4 g (36.4 wt%), galanga root 16.4 g (16.4 wt%), soapwort 7.2 g (7.2 wt%), Larea Mexicana 3.6 g (3.6 wt%). That is, artichoke leaves, Chistus monspeliensis, galanga root, soap grass, and Larea mexicana were mixed at a weight ratio of 10.1:10:1:4.6:2:1.

1,000 ml of distilled water was added to the mixed raw materials as above, and Viscozyme L (Viscozyme L) at a concentration of 1% was added to it.L) was added and extracted at 50°C for 8 hours. Afterwards, the separated extract was filtered through a 1.0 μm filter to obtain the complex extract of the present invention, which was then used in the following test.

Comparative Example 1.

1,000 ml of distilled water was added to 100 g (total weight) of the raw material containing artichoke leaves, Chistus monspeliensis, galanga root, soap grass, and Larea mexicana prepared in the same composition ratio as in Example 1, and then 90% without enzyme. Hot water extraction was performed at ℃ for 8 hours, and the separated extract was obtained by filtering through a 1.0 μm filter and used in the following test.

Comparative Example 2.

Mix 25g (25% by weight), 25g (25% by weight), 25g (25% by weight), and 25g (25% by weight) of artichoke leaves, Chistus monspeliensis, galanga root, and soap grass, respectively, for a total weight of 100g. A mixture of was prepared. That is, artichoke leaves, Chistus monspeliensis, galanga root, and soap grass were mixed at a weight ratio of 1:1:1:1. 1,000 ml of distilled water was added to the mixed raw materials, and 1% concentration of Viscozyme L (ViscozymeAfter addition of L), extraction was performed at 50°C for 8 hours, and the separated extract was filtered through a 1.0 μm filter to obtain a complex extract. The extract thus obtained was used in the following tests.

Comparative Example 3.

Mix 25g (25% by weight), 25g (25% by weight), 25g (25% by weight), and 25g (25% by weight) of artichoke leaves, Chistus monspeliensis, galanga root, and Larea mexicana, respectively. A mixture weighing 100 g was prepared. That is, artichoke leaves, Chistus monspeliensis, Galanga root, and Larea Mexicana were mixed at a weight ratio of 1:1:1:1. 1,000 ml of distilled water was added to the mixed raw materials, and 1% concentration of Viscozyme L (ViscozymeAfter addition of L), extraction was performed at 50°C for 8 hours, and the separated extract was filtered through a 1.0 μm filter to obtain a complex extract. The extract thus obtained was used in the following tests.

Comparative Example 4.

Mix 25g (25% by weight), 25g (25% by weight), 25g (25% by weight), and 25g (25% by weight) of artichoke leaves, Chistus monspeliensis, soap grass, and Larea mexicana, respectively. A mixture weighing 100 g was prepared. That is, artichoke leaves, Chistus monspeliensis, soap grass, and Larea mexicana were mixed at a weight ratio of 1:1:1:1. 1,000 ml of distilled water was added to the mixed raw materials, and 1% concentration of Viscozyme L (ViscozymeAfter addition of L), extraction was performed at 50°C for 8 hours, and the separated extract was filtered through a 1.0 μm filter to obtain a complex extract. The extract thus obtained was used in the following tests.

Comparative Example 5.

Mix 25g (25% by weight), 25g (25% by weight), 25g (25%), 25g (25% by weight) of artichoke leaves, galanga roots, soap grass, and Larea mexicana, respectively, for a total weight of 100g. The mixture was prepared. That is, artichoke leaves, galanga roots, soap grass, and Larea mexicana were mixed at a weight ratio of 1:1:1:1. 1,000 ml of distilled water was added to the mixed raw materials, and 1% concentration of Viscozyme L (ViscozymeAfter addition of L), extraction was performed at 50°C for 8 hours, and the separated extract was filtered through a 1.0 μm filter to obtain a complex extract. The extract thus obtained was used in the following tests.

Comparative Example 6.

Total weight of 25g (25% by weight), 25g (25% by weight), 25g (25% by weight), and 25g (25% by weight) of Chistus monspeliensis, galanga root, soap plant, and Larea mexicana, respectively. 100 g of mixture was prepared. That is, Chistus monspeliensis, galanga root, soap plant, and Larea mexicana were mixed at a weight ratio of 1:1:1:1. 1,000 ml of distilled water was added to the mixed raw materials, and 1% concentration of Viscozyme L (ViscozymeAfter addition of L), extraction was performed at 50°C for 8 hours, and the separated extract was filtered through a 1.0 μm filter to obtain a complex extract. The extract thus obtained was used in the following tests.

Comparative Example 7.

20 g (20% by weight), 20 g (20% by weight), 20 g (20% by weight), 20 g (20% by weight) of artichoke leaves, Chistus monspeliensis, galanga root, soap plant and Larea mexicana, respectively, 20g (20% by weight) were mixed to prepare a mixture with a total weight of 100g. That is, artichoke leaves, Chistus monspeliensis, galanga root, soap grass, and Larea mexicana were mixed at a weight ratio of 1:1:1:1:1. 1,000 ml of distilled water was added to the mixed raw materials, and 1% concentration of Viscozyme L (ViscozymeAfter addition of L), extraction was performed at 50°C for 8 hours, and the separated extract was filtered through a 1.0 μm filter to obtain a complex extract. The extract thus obtained was used in the following tests.

Test Example 1: Extraction Yield

In order to confirm the extraction yield of the artichoke leaf, Chistus monspeliensis, galanga root, soap grass and Larea mexicana complex extract prepared in Example 1, the complex extract of Example 1 and the extraction of Comparative Example 1 Yields were comparatively analyzed.

As a result, as can be seen in Table 1, it was observed that the total extraction yield of Example 1 was improved by about 15.19% compared to Comparative Example 1.

Through the above results, it was confirmed that the method for producing complex extracts of artichoke leaves, Chistus monspeliensis, Galanga root, soap grass and Larea mexicana according to the present invention shows improved extraction yield.

Extraction Yield Comparative Example 1 (90°C, 8 hours, hot water extraction) 20.95% Example 1 (Viscozyme L 1%, 50°C, 8 hours) 36.14%

Test Example 2. Cytotoxicity

In order to confirm the toxicity of the artichoke leaf, Chistus monspeliensis, Galanga root, soap grass and Larea mexicana complex extract prepared in Example 1 and each raw material to cells, the extract and each raw material were treated. Afterwards, cell viability was confirmed through MTT analysis.

Specifically, HaCaT cells were distributed in a 96-well plate at 2x10 ⁴ cells/well. DMEM medium (Gibco, 11965-092) supplemented with 10% FBS (Gibco, 16000-044) and 1% Penicillin-Streptomycin (Sigma, P4458) was used as a medium for culturing cells, and was incubated at 37°C for 5 days. Cultured for one day in a % CO ₂ incubator. After 24 hours, all medium supernatant was removed, and then 180 μl of new HaCaT-culture medium containing the sample at a concentration of 2.0 wt% was added and cultured in a 5% CO ₂ incubator at 37°C for 24 hours.

Add 20 μl of MTS (Promega, G3581, Cell titer 96 Aqueous One Solution), react for 2 hours in a 5% CO ₂ incubator at 37°C, and then use Envision (Perkin-Elmer, A485-MTS assay 96-well) The cell number was measured.

As can be seen in Figure 1, when the extract according to the present invention was treated with human keratinocytes at a concentration of 2.0% by weight, there was no significant difference in cell viability compared to the negative control using DMSO, and the cell viability was more than 90%. indicated.

That is, the extract according to the present invention does not affect the survival rate of human keratinocytes even when used at high concentration. In addition, it was confirmed that there was no cytotoxicity even when treating each single extract.

Test Example 3. Inflammatory cytokine (IL-6 and IL-8) inhibition ability

In order to confirm the inhibitory ability of the artichoke leaf, Chistus monspeliensis, Galanga root, Soapwort and Larea Mexicana complex extract prepared in Example 1 on inflammatory cytokines, inflammatory cytokines secreted by human keratinocytes were tested. After stimulating the HaCaT keratinocyte cell line using the cytokines Interferon-gamma (IFN-γ) and Tumor necrosis factor-alpha (TNF-α), the extract was processed to induce changes in inflammatory cytokines IL-6 and IL-8. Confirmed.

Specifically, HaCaT cells were distributed in a 96-well plate at 2x10 ⁴ cells/well. As a medium for culturing cells, DMEM medium (Gibco, 11965-092) supplemented with 10% FBS (Gibco, 16000-044) and 1% Penicillin-Streptomycin (Sigma, P4458) was used, and 5% CO at 37°C. It was cultured in a CO ₂ incubator for one day. After 24 hours, all medium supernatant was removed, and then 200 μl of new HaCaT-culture medium containing 2.0 wt% concentration was added and re-cultured. After 30 minutes, 10 ng/mL each of IFN-γ and TNF-α as stimulation substances were added and cultured for one day. After 24 hours, only the medium supernatant was recovered, and the contents of IL-6 and IL-8 released in the supernatant were measured. At this time, a standard curve prepared using recombinant human protein provided by the manufacturer was used to measure the content.

As can be seen in Figure 2, it was confirmed that in HaCaT cells cultured with IFN-γ and TNF-α, the complex extract according to the present invention inhibits the production of IL-6, a substance that mediates the inflammatory response.

Additionally, as can be seen in Figure 3, it was confirmed that in HaCaT cells cultured with IFN-γ and TNF-α, the complex extract according to the present invention inhibits the production of IL-8, a substance that mediates the inflammatory response.

Therefore, it can be seen that the complex extract according to the present invention has the effect of alleviating inflammation and skin barrier damage on human keratinocytes by inhibiting the production of inflammatory cytokines IL-6 and IL-8.

Claims (8)

A cosmetic comprising extracts of artichoke (Cynara Scolymus) leaves, Cistus Monspeliensis (Cistus Monspeliensis), Galanga (Kaempferia Galanga) root, Saponaria Officinalis and Larrea Mexicana as active ingredients. Composition. According to paragraph 1,
The composition is an extract of artichoke leaves 5 to 15, Chistus monspeliensis 5 to 15, galanga root 1 to 10, soapwort 1 to 3 and Larea mexicana mixed in a weight ratio of 0.5 to 1.5. According to paragraph 1,
The composition is wherein the extract is extracted from one or more solvents selected from the group consisting of water, methanol, ethanol, propyl alcohol, butyl alcohol, glycerol, propanediol butylene glycol, propylene glycol, and 1,2-hexanediol. According to paragraph 1,
A composition wherein the extract inhibits the secretion of inflammatory cytokines. According to paragraph 1,
A composition in which the extract relieves skin irritation. First step of preparing a mixture by mixing artichoke (Cynara Scolymus) leaves, Cistus Monspeliensis, Galanga (Kaempferia Galanga) roots, Saponaria Officinalis and Larrea Mexicana ;
A second step of preparing an extract by adding a solvent and a hydrolytic enzyme to the mixture of the first step; and
A method for producing a cosmetic composition comprising a third step of filtering the extract of the second step through a filter to obtain a composition. delete delete