KR102593399B1 - Microbial fertilizer additive composition using citrus juice and method for preparing the same - Google Patents

Abstract

A microbial feed additive composition using citrus juice and a method for manufacturing the same are disclosed. The feed additive according to one embodiment has the effect of removing odor, maximizing feed performance, and increasing the population of microorganisms.

Description

Microbial fertilizer additive composition using citrus juice and method for preparing the same}

The examples below relate to a microbial feed additive composition using citrus juice and its manufacturing method technology.

Due to a lack of feed resources, Korea relies on foreign imports for more than 75% of the total amount of feed fed to livestock and more than 95% for feed grains. Moreover, due to the recent rise in the exchange rate, a sharp rise in international feed grain prices and the coronavirus outbreak, Difficulties are becoming more severe. Therefore, in order to respond to the full import opening of livestock products following the conclusion of the UR negotiations and to enhance the international competitiveness of domestically produced livestock products, it is essential to dramatically reduce feed costs, which account for most of the production costs of livestock products. To this end, self-development of cheap existing feed resources is necessary. This is a very urgent proposal. One of the most feasible alternatives to this is turning organic waste into livestock feed. In general, unlike industrial waste, organic waste has little contamination with heavy metals and has a high content of organic nutrients, making it highly usable as livestock feed.

Jeju Island's feed prices tend to be higher than on the mainland due to the high price of raw materials due to the price of raw materials plus transportation costs due to geographical requirements. Therefore, organic waste from Jeju's food factories can be recycled and reused as feed, which is good for the environment and can have the effect of reducing feed costs. As the number of resource purification projects increases in the United States, Canada, and Japan, environmentally conscious recycling projects are becoming more active.

About 450,000 tons of tangerines are produced in Jeju every year, and 20% of them are discarded as pachis and produced as tangerine juice at juice factories. However, 10,000 tons of juice are discarded during the production process. These discarded juices are currently being treated at wastewater treatment facilities, and the cost is also increasing significantly.

At the Jeju Development Corporation and private citrus juice factories, waste generated during the citrus juice production process is divided into two types: citrus peels and citrus juice. However, there are realistic limitations in processing these wastes every year, so a method of utilizing these wastes is needed. Citrus peel can be used as a raw material for cattle feed or feed, but citrus juice is 100% treated for wastewater purification using activated sludge.

To date, there are many farms that spray urea dioxide in pig pens, but urea dioxide is a chemical product and results in the death of all microorganisms. Although spraying microorganisms takes a long time, it is expected to be effective as it can be a fundamental measure.

Sugar is the food source for microorganisms, and molasses is most commonly used. Molasses is a by-product left after sugar is extracted from sugar cane or sugar beets and is entirely dependent on imports. And in Jeju Island, there is a problem of having to purchase in bulk quantities of 25 or 50 tons at a time. Therefore, many farmers in Jeju either use sugar or glucose, even though it is expensive, or give up cultivating microorganisms.

Citrus juice has a sugar content of 9 Brix, which is sufficient for microorganisms to grow. And as the citrus scent appears when sprayed in the air due to the effect of citrus juice, the satisfaction of customers is also increasing.

In this situation, the present inventors tried to develop a feed additive by cultivating microorganisms using citrus juice, and it can be added as sweet feed or supplementary feed to remove bad odors, increase beneficial bacteria, and improve feed quality. We have made every effort to provide additives that are available.

1. KR20170076360A 2. KR101806156B1

The embodiments aim to provide a feed additive manufactured by cultivating microorganisms using citrus juice. In particular, the purpose is to provide additives that can eliminate bad odors, increase beneficial bacteria, and improve feed quality.

Meanwhile, we aim to increase natural recycling by developing microbial preparations using Jeju's natural resources.

Furthermore, we aim to provide standardization for mass production of microbial preparations. Providing a manual is important in large-scale expansion, and we aim to provide optimization measures such as sterilization work before microbial inoculation, selection of medium, and selection of medium according to pH acidity.

In addition, we aim to provide a feed additive with increased composting effect when applied to pig farms' feed, compost, and liquid manure. For this purpose, we attempted to measure the composting power of compost and liquid fertilizer and confirm the number of microorganisms.

Furthermore, we aim to provide feed additives that can be applied to smart farms in citrus farms.

Furthermore, we attempted to measure the odor status of feed, compost, and liquid fertilizer in connection with weather and climate changes.

The present invention to solve the above problems,

Preparing citrus juice with a sugar content of 8 to 10.5 Brix;

Adjusting the pH of the citrus juice to 5.5 to 6.5;

Comprising the step of inoculating the citrus juice with microorganisms and culturing them at 30 to 40°C for 36 to 60 hours to provide a culture medium,

A method for manufacturing a microbial feed additive composition is provided.

According to one embodiment, the microorganism may be a combination of Bacillus subtilis, yeast, and photosynthetic bacteria.

According to one embodiment, in the step of adjusting the pH of the citrus juice, the pH is adjusted to 5.5 to 6.5 by adding at least one pH adjuster selected from the group consisting of sodium hydroxide, sodium bicarbonate, and ammonium chloride. You can.

According to one embodiment, providing citrus juice with a sugar content of 8 to 10.5 Brix;

adjusting the pH to 5.5 to 6.5 by adding at least one pH adjuster selected from the group consisting of sodium hydroxide, sodium bicarbonate, and ammonium chloride to the citrus juice;

Inoculate 0.01 to 1.0 parts by weight of 400 to 60 billion cfu/g of each of the Bacillus subtilis , Saccharomyces cerevisiae, and Rhodobacter azotofomans strains into 70 parts by weight of the citrus juice and incubate at 30 to 40°C for 36 to 60 hours to provide a culture medium. It may include steps of:

According to one embodiment, providing citrus juice with a sugar content of 8 to 10.5 Brix;

adjusting the pH to 5.5 to 6.5 by adding at least one pH adjuster selected from the group consisting of sodium hydroxide, sodium bicarbonate, and ammonium chloride to the citrus juice;

Inoculate 0.05 to 0.2 parts by weight of 45 to 55 billion cfu/g of each of the Bacillus subtilis MG401, Saccharomyces cerevisiae atcc9763, and Rhodobacter azotofomans ATCC 17025 strains into 70 parts by weight of the citrus juice and incubate at 30 to 40°C for 36 to 60 hours. This may include the step of providing a culture medium.

The device according to one embodiment may be combined with hardware and controlled by a computer program stored in a medium to execute any one of the above-described methods.

The feed additives of the examples have the effect of removing odor, maximizing feed performance, and increasing microbial population.

In addition, natural circulation is possible using citrus juice, which helps in resource recycling. Because it has a citrus scent, you can get a flavor effect and increase the sugar content.

Furthermore, instead of using molasses as food for microorganisms, the sugar contained in citrus fruits is used, which can reduce costs and replace imported products.

Additionally, it is possible to standardize mass production of citrus juice probiotics.

1 is a schematic diagram of the manufacturing method of the present invention.
Figure 2 is a diagram showing the microbial production line of Jeju Urban Development Corporation's first factory, which performs microbial inoculation and culture processes.
Figure 3 is a diagram showing a scene of inoculating a feed additive composition when producing compost and liquid fertilizer after collecting raw materials (left: compost, right: liquid fertilizer).
Figure 4 is a diagram showing ammonia and hydrogen sulfide measurements.
Figure 5 shows the results showing the degree of decomposition and microbial multiplier of compost and liquid manure by applying the feed additive of Example 1 to farm A.
Figure 6 shows the results showing the degree of decomposition and microbial multiplier of liquid manure by applying the feed additive of Example 1 to farm B.
Figure 7 shows the results showing the degree of spoilage and microbial multiplier of liquid manure by applying the feed additive of Example 1 to farm C.

Hereinafter, embodiments will be described in detail with reference to the attached drawings. However, various changes can be made to the embodiments, so the scope of the patent application is not limited or limited by these embodiments. It should be understood that all changes, equivalents, or substitutes for the embodiments are included in the scope of rights.

Specific structural or functional descriptions of the embodiments are disclosed for illustrative purposes only and may be modified and implemented in various forms. Accordingly, the embodiments are not limited to the specific disclosed form, and the scope of the present specification includes changes, equivalents, or substitutes included in the technical spirit.

Terms such as first or second may be used to describe various components, but these terms should be interpreted only for the purpose of distinguishing one component from another component. For example, a first component may be named a second component, and similarly, the second component may also be named a first component.

When a component is referred to as being “connected” to another component, it should be understood that it may be directly connected or connected to the other component, but that other components may exist in between.

The terms used in the examples are for descriptive purposes only and should not be construed as limiting. Singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, terms such as “comprise” or “have” are intended to designate the presence of features, numbers, steps, operations, components, parts, or combinations thereof described in the specification, but are not intended to indicate the presence of one or more other features. It should be understood that this does not exclude in advance the possibility of the existence or addition of elements, numbers, steps, operations, components, parts, or combinations thereof.

Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as generally understood by a person of ordinary skill in the technical field to which the embodiments belong. Terms defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and unless explicitly defined in the present application, should not be interpreted in an ideal or excessively formal sense. No.

The advantages and features of the present invention and methods for achieving them will become clear by referring to the embodiments described in detail below along with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below and will be implemented in various different forms. The present embodiments only serve to ensure that the disclosure of the present invention is complete and that common knowledge in the technical field to which the present invention pertains is not limited. It is provided to fully inform those who have the scope of the invention, and the present invention is only defined by the scope of the claims.

In the embodiments of the present invention, unless otherwise defined, all terms used herein, including technical or scientific terms, are the same as those commonly understood by a person of ordinary skill in the technical field to which the present invention pertains. It has meaning. Terms defined in commonly used dictionaries should be interpreted as having a meaning consistent with the meaning in the context of the related technology, and unless clearly defined in the embodiments of the present invention, have an ideal or excessively formal meaning. It is not interpreted as

The shapes, sizes, proportions, angles, numbers, etc. disclosed in the drawings for explaining embodiments of the present invention are illustrative, and the present invention is not limited to the matters shown. Additionally, in describing the present invention, if it is determined that a detailed description of related known technologies may unnecessarily obscure the gist of the present invention, the detailed description will be omitted. When 'includes', 'has', 'consists of', etc. mentioned in this specification are used, other parts may be added unless 'only' is used. When a component is expressed in the singular, the plural is included unless specifically stated otherwise.

When interpreting a component, it is interpreted to include the margin of error even if there is no separate explicit description.

The size and thickness of each component shown in the drawings are shown for convenience of explanation, and the present invention is not necessarily limited to the size and thickness of the components shown.

Each feature of the various embodiments of the present invention can be partially or fully combined or combined with each other, and as can be fully understood by those skilled in the art, various technical interconnections and operations are possible, and each embodiment may be implemented independently of each other. It may be possible to conduct them together due to a related relationship.

Hereinafter, the configuration of the present invention and its effects will be described in more detail through specific examples and comparative examples. However, these examples are for illustrating the present invention in more detail, and the scope of the present invention is not limited to these examples.

Examples and comparative examples. Preparation of microbial feed additive composition

Preparation of citrus juice

During the citrus juice production process, the waste liquid left over from the juice was prepared (the citrus juice itself can be used). The sugar content was 9 Brix, but you can use one with 8 to 10.5 Brix and you can concentrate or dilute it to adjust the sugar content. The prepared citrus juice was sterilized using UV.

Media input

Sodium hydroxide, sodium bicarbonate, and ammonium chloride were added to the citrus juice to adjust the pH to 6.

Microbial inoculation and culture

The present inventors sought to identify a starter that decomposes citrus juice and allows microorganisms to grow well.

Based on 70kg of citrus juice, starter was added in the following combination and fermented in a microbial incubator at a temperature of 35°C for 48 hours. Figure 2 is a diagram showing the microbial production line of Jeju Urban Development Corporation's first factory that performs this process.

Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Example 1 Bacillus subtilis MG401 (50 billion cfu/g) 0.3kg Bacillus subtilis MG401 (50 billion cfu/g) 0.15kg Bacillus subtilis MG401 (50 billion cfu/g) 0.1kg Bacillus subtilis MG401 (50 billion cfu/g) 0.1kg Bacillus subtilis MG401 (50 billion cfu/g) 0.1kg Saccharomyces cerevisiae atcc9763 (50 billion cfu/g) 0.15kg Lactobacillus plantarum MG207 (50 billion cfu/g), 0.1kg Saccharomyces cerevisiae atcc9763 (50 billion cfu/g) 0.1kg Saccharomyces cerevisiae atcc9763 (50 billion cfu/g) 0.1kg Rhodobacter azotofomans ATCC 17025 (50 billion cfu/g) 0.1kg Lactobacillus plantarum MG207 (50 billion cfu/g), 0.1kg Rhodobacter azotofomans ATCC 17025 (50 billion cfu/g) 0.1kg

MG401, MG207 provided by Mediogen Co., Ltd.

Packaging stage

The cultured medium was concentrated, freeze-dried, and then packaged.

Experimental Example 1. Deodorization and composting performance

The deodorizing performance of the feed additives prepared in the above Examples and Comparative Examples against various odors was tested. Specifically, 20 ml of sample was placed in a 5 liter reactor and sealed. The initial concentration of the test gas was 100 μmol/mol (ppm) for ammonia, 30 μmol/mol (ppm) for trimetalamine, 50 μmol/mol (ppm) for hydrogen sulfide, and 4 μmol/mol (ppm) for methyl mercaptan. After injection, the concentration of the test gas was measured 30 minutes later and was called the sample concentration. The concentration of the test gas was measured according to KS I 2218:2009. During the test, the temperature was maintained at 23°C ± 5°C and the humidity was maintained at 50% ± 10%. Separately, the same test as above was performed in the absence of a sample and this was referred to as blank concentration. The removal rate of the test gas for each time period was calculated using the following equation.

Deodorization rate of test gas (%) = [{(blank concentration)-(sample concentration)/(blank concentration)}

The test method was performed using the gas detection tube method (KS I 2218). The results are shown in Table 2 below.

Ammonia deodorization rate (%) Triethylamine deodorization rate (%) Hydrogen sulfide deodorization rate (%) Methyl mercaptan deodorization rate (%) Example 1 100 100 70 40 Comparative Example 1 87 72 41 29 Comparative Example 2 81 82 30 21 Comparative Example 3 83 84 35 26 Comparative Example 4 85 87 38 28

As a result, the feed additive of Example 1, ammonia (NH ₃ ) and triethylamine (CH ₃ ) ₃ N), removed almost 100% of odor within 30 minutes of treatment. In addition, hydrogen sulfide (H ₂ S) removed more than 70% of bad odors, and methyl mercaptan (CH ₃ SN) removed more than 40% of bad odors. Therefore, it was confirmed that the feed additive of the present invention can achieve a high deodorizing effect against various livestock odors. In particular, as a result of comparison with comparative examples, it was confirmed that the effect was due to selection of an appropriate strain.

Feed with added feed additives was fed to laying hens, egg production rate and egg quality were compared, and the results are shown in Table 3. Laying hens that were not fed the feed additive of the present invention were used as the control group. In addition, samples were collected 24 hours after the feed additive was added and the number of Bacillus subtilis bacteria was measured.

Spawning rate (%) Blue rate (%) Bacillus subtilis count control group 76 3.0 1.5×10 ⁷ CFU/g Example 1 89 2.1 3.1×10 ⁸ CFU/g Comparative Example 1 81 2.6 1.6×10 ⁸ CFU/g Comparative Example 2 83 2.5 1.2×10 ⁸ CFU/g Comparative Example 3 82 2.7 1.8×10 ⁸ CFU/g Comparative Example 4 85 2.7 2.1×10 ⁸ CFU/g

As described above, in Example 1, it was confirmed that the scattering rate was high and the blue rate was low. It was also confirmed that the content of beneficial bacteria was high. On the other hand, the comparative examples did not have an excellent scattering effect and showed a low number of microorganisms. Accordingly, the excellent effect of the example appears to be the effect of selecting an appropriate strain.

Experimental Example 2. Results of application to pig farms

The microbial feed additive composition of Example 1 was applied to compost raw materials and liquid fertilizer raw materials collected from a pig farm.

Specifically, liquid fertilizer raw materials were collected from the water collection tank spaces of three pig farms A, B, and C in Hallim, and the microbial feed additive composition of Example 1 was applied. In addition, the microbial feed additive composition of the above examples and comparative examples was sprayed on compost raw materials that had passed through a solid-liquid separator at farm A, and the degree of decomposition was measured.

Samples were collected before and after composting, and the degree of composting and number of microbial survivals were measured. In addition, odors of ammonia and hydrogen sulfide were also measured immediately before collecting samples for measurement.

Figure 3 is a diagram showing a scene of inoculating a feed additive composition when producing compost and liquid fertilizer after collecting raw materials (left: compost, right: liquid fertilizer). Figure 4 is a diagram showing ammonia and hydrogen sulfide measurements.

(One) Results from Farm A

First, the results showing the degree of decomposition and microbial multiplier of compost and liquid manure at farm A are shown in Figure 5 and Table 4.

(Unit: ppm)

In this way, the level of spoilage and microbial multiplier were shown to significantly increase in the raised pig pens and water collection tanks of Farm A. It appears that the feed additive composition of the present invention has a stable degree of decomposition and is effective in producing compost and liquid fertilizer. When the first sample was collected, the degree of spoilage was 0%, but in the last sample collected, the value was 85%, and the amount of Bacillus subtilus continued to increase.

In addition, ammonia and hydrogen sulfide levels are improving over time, and levels below 15ppm are considered excellent results as they represent well-managed pig farms. When the farm manager entered the pig pen, it was confirmed that no eye irritation occurred and that the incidence of respiratory diseases in the pigs was reduced during the test period.

(2) Results at Farm B

Next, the results showing the degree of spoilage and microbial multiplier of liquid manure in farm B are shown in Figure 6 and Table 5.

(Unit: ppm)

In farm B, the composting ability of the liquid fertilizer was reduced due to a malfunction of the aeration facility in the aeration tank in the middle of the experiment period. Nevertheless, it was confirmed that the boiling ability was restored. In addition, the results showed that the survival rate of Bacillus subtilus was increased in liquid fertilizer.

In farm B, ammonia and hydrogen sulfide levels continued to decrease, and although the composting power fluctuated due to the aeration facility, it did not affect the odor level, and the ammonia and hydrogen sulfide values were 0 ppm after the 4th round.

(3) Results from C Farm

Finally, the results showing the degree of spoilage and microbial multiplier of liquid manure in farm C are shown in Figure 7 and Table 6.

(Unit: ppm)

Farm C was newly expanded a year ago and was well equipped, but microbial inoculation had never been applied. Inoculation with the feed additive of the present invention dramatically increased the multiplier of microorganisms and showed stable results.

Farm C was a new pig farm, so the odor was not a major problem at first. As shown in the table above, the values of ammonia and hydrogen sulfide dropped to 0 ppm.

From the results shown in the farms as above, both the rotting degree and the microbial multiplier increased, the odor problem decreased, and excellent results were shown.

Claims (3)

Providing citrus juice with a sugar content of 8 to 10.5 Brix;
adjusting the pH to 5.5 to 6.5 by adding at least one pH adjuster selected from the group consisting of sodium hydroxide, sodium bicarbonate, and ammonium chloride to the citrus juice;
Inoculate 0.05 to 0.2 parts by weight of 45 to 55 billion cfu/g of each of the Bacillus subtilis MG401, Saccharomyces cerevisiae atcc9763, and Rhodobacter azotofomans ATCC 17025 strains into 70 parts by weight of the citrus juice and incubate at 30 to 40°C for 36 to 60 hours. Including the step of providing a culture medium,
Method for producing a microbial feed additive composition for pig farming.



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