KR102571999B1 - Cosmetic composition for improving skin vitality and skin troubles - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising bakuchiol, niacinamide, and ascorbic acid or a derivative thereof as active ingredients, which may further include panthenol, a jacaranda flower extract, or a mixture of panthenol and a jacaranda flower extract, It can soothe the skin, improve skin troubles, and give vitality to the skin to create a lively skin.

Description

Cosmetic composition for improving skin vitality and improving skin troubles {Cosmetic composition for improving skin vitality and skin troubles}

The present invention relates to a cosmetic composition for enhancing skin vitality and improving skin troubles.

Skin diseases refer to all diseases occurring in the skin, and their types include atopic dermatitis, acne, eczema, tumors, hives, psoriasis, melasma, freckles, shingles, milia, folliculitis, and facial flushing. . Skin troubles arise from various causes. They may occur due to the collapse of the skin barrier or the unbalanced secretion of hormones due to various external environments, or may occur when the elasticity of the skin is reduced due to a lack of nutrition and oxygen supply.

Acne, a typical skin problem, is an inflammatory disease caused by excessive secretion of sebum and not being properly discharged from pores. It appears in places where sebaceous glands are developed, such as the back and chest. During puberty, as the secretion of sebum increases due to the rapid increase in secretion of hormones, inflammatory substances are created in the skin, resulting in acne concentrated in the T-zone area, cheeks, forehead, forehead, and nose where sebum is relatively high. It appears, and it takes on the aspect of inflammation or purulent acne. In adulthood, acne occurs for various reasons, such as excessive stress, cosmetic residues, irregular sleep patterns, and wrong eating habits. As the waste discharge passage narrows, wastes accumulate in the skin and often progress to skin inflammation.

Atopic dermatitis is a skin eczema disease that accompanies severe itching and recurs chronically, and is one of the representative allergic diseases. It is known that this is caused by a combination of various factors, such as genetic predisposition, environmental factors, immunological abnormalities, and skin barrier abnormalities. The importance of environmental factors is emphasized. Although atopic dermatitis has been recognized as a pediatric disease, the incidence rate in adults continues to increase in recent years, which is presumed to be caused by genetic tendencies, external environment and mental stress. In particular, adult atopy appears on exposed areas such as the face and neck, and the severity is severe. In the case of atopic patients, secondary damage is also occurring, such as losing confidence in interpersonal relationships or complaining of depression in times of increased exposure, such as spring and summer.

On the other hand, as wearing a mask has become commonplace due to the recent novel coronavirus infection-19 (COVID-19) pandemic, people who complain of various skin diseases such as pimples, troubles, inflammation, and itching are increasing. When wearing a mask, moisture and heat are generated due to breathing, and the inside of the mask maintains a high-temperature and humid environment. It creates an environment that is easy for germs that exist on the skin, such as staphylococcus, to multiply. Moreover, an increase in skin temperature also widens pores, and skin heat may cause or worsen rosacea by acting as a direct stimulus to the skin. In addition, fine scratches caused by repeated friction with the mask not only damage the skin barrier, but can also cause secondary bacterial infection and contact dermatitis, and the pressure caused by the mask stimulates hair follicles and further exacerbates inflammation. can In addition, if hygiene management of the mask is insufficient, such as repeated use of the mask, it can be a direct cause of skin diseases such as acne and folliculitis, as bacterial propagation becomes easier.

In addition, skin troubles are also affected by environmental pollution such as fine dust, and excessive secretion of sebum and abnormal keratinization become more severe in summer with hot and humid weather. Sebum secretion rises by about 10% when the temperature rises by 1℃ As a result, dead skin cells entangled in sebum do not achieve normal keratinization and clog pores, increasing the risk of skin troubles such as acne or eczema.

As problems with sensitive skin due to wearing masks and external stimuli diversify, interest in functional cosmetics with skin trouble improvement and skin soothing functions is on the rise. Accordingly, cosmetics with effects such as improvement of skin diseases and relief of skin troubles are being developed. For example, Korean Patent Publication No. 10-2016-0025952 discloses copper tripeptide-1 and an amino acid mixture as active ingredients. Korean Patent Registration No. 10-2047193 discloses a cosmetic composition for treating skin troubles containing glutamylamidoethylindole as an active ingredient. However, a lot of research is being done to realize better efficacy, and the recent craze for clean beauty and vegan cosmetics that considers good ingredients and the environment has increased the need for plant ingredients, reducing side effects from natural resources, including plants, and being harmless to the human body. However, there is a need to develop products with excellent efficacy.

An object of the present invention is to provide a cosmetic composition having excellent skin vitality enhancement and skin trouble relief effects and cosmetics using the same.

The present invention to achieve the above object bakuchiol (bakuchiol); niacinamide; And ascorbic acid (ascorbic acid) or a derivative thereof; to provide a cosmetic composition for promoting skin vitality and improving skin troubles containing as an active ingredient.

In addition, the composition may further include panthenol, jacaranda flower extract, or a mixture of panthenol and jacaranda flower extract.

The ascorbic acid derivative may be sodium ascorbyl phosphate, magnesium ascorbyl phosphate, ascorbyl glucoside or aminopropyl ascorbyl phosphate.

The skin trouble may be selected from the group consisting of atopic dermatitis, pimple, skin rash, rosacea, psoriasis, eczema, seborrheic dermatitis, erythematous disease, allergic pruritus, acne, bullous dermatosis and pemphigus vulgaris. .

The bakuchiol may be a bogolji extract, and the bakuchiol may be included in an amount of 0.01 to 13% by weight based on the total weight of the composition.

The niacinamide may be included in an amount of 0.01 to 10% by weight based on the total weight of the composition, and the ascorbic acid may be included in an amount of 0.1 to 15% by weight based on the total weight of the composition.

The panthenol, jacaranda flower extract, or a mixture of panthenol and jacaranda flower extract may be included in an amount of 0.01 to 8% by weight based on the total weight of the composition.

In addition, the composition may be formulated as any one selected from the group consisting of lotion, essence, serum, lotion, gel, paste, cream, powder, oil, pack, mist, soap, cleanser, makeup base, and foundation.

The cosmetic composition according to the present invention contains bakuchiol, niacinamide, and ascorbic acid or a derivative thereof as active ingredients to soothe the skin and improve skin troubles, and can give vitality to the skin to realize a lively skin. . In addition, it is hypoallergenic and can be applied to sensitive skin, including bakuchiol, which is a vegetable ingredient, so it can be usefully applied to skin care.

1 shows the moisturizing effect test results according to an embodiment of the present invention.

Hereinafter, the present invention will be described in more detail.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and correspond to the meanings commonly understood by those of ordinary skill in the art related to the present invention. In addition, although preferred methods or samples are described in this specification, those similar or equivalent thereto are also included in the scope of the present invention.

Throughout this specification, unless the context requires otherwise, the terms "comprise" and "comprising" include a given step or component, or group of steps or components, but any other step or component, or It is to be understood that steps or groups of components are not excluded.

In the present specification, “active ingredient” means a component that exhibits the desired activity alone or can exhibit the desired activity together with a carrier having no activity itself.

In the present invention, "skin trouble" means a skin disease that can be caused by inflammation caused by excessive production of nitric oxide in macrophages, and any skin disease related to inflammation is included regardless of its type. Non-limiting examples of skin diseases related to the inflammation include atopic dermatitis, psoriasis, erythematous diseases triggered by radiation, chemicals, burns, etc., acid burns, blistering dermatosis, lichenoid diseases, itching caused by allergies, seborrheic Eczema, rosacea acne, pemphigus vulgaris, erythema multiforme exudative, erythema nodosum, balanitis, vulvitis, inflammatory hair loss such as alopecia areata, cutaneous T-cell lymphoma, etc., specifically, atopic dermatitis, skin rash, acne, pimples, etc., but is not limited thereto.

In addition, the term "trouble improvement" used in the present invention means suppressing or inhibiting the generation of skin troubles, or alleviating already generated troubles.

In addition, the term "skin vitality" as used in the present invention refers to the degree to which skin cells are activated, and "skin vitality promotion" means that the skin can be revitalized by enhancing the activation of skin cells.

The present invention bakuchiol (bakuchiol); niacinamide; And ascorbic acid (ascorbic acid) or a derivative thereof; to provide a cosmetic for promoting skin vitality and improving skin troubles containing as an active ingredient.

In addition, the present invention provides a cosmetic composition for enhancing skin vitality and improving skin troubles, further comprising panthenol, a jacaranda flower extract, or a mixture of panthenol and a jacaranda flower extract.

First, each component included in the composition according to the present invention will be described.

Bakuchiol is a vegetable component extracted from the leaves and seeds of Psoralea corylifolia and is a meroterpene compound of the terpenophenol family. Bakuchiol has long been recognized for its antioxidant and anti-inflammatory effects and has been used for the treatment of various diseases in traditional medicine in India and China.

Bakuchiol has been reported to have anticancer activity in preclinical models due to its structural similarity with resveratrol (Chen, Zhe et al., 2010, European Journal of Pharmacology. 643 (2-3): 170-9), In a study on rats, it was reported that the ethanol extract and bakuchiol of the Chinese medicinal plant P. corylifolia prevented bone loss (Lim, Sun-Hye et al., 2008, British Journal of Nutrition. 101 (7) : 1031-1039). Bakuchiol also has antioxidant activity (Adhikari, S. et al., 2003, Chemical Research in Toxicology. 16 (9): 1062-1069; Haraguchi, Hiroyuki et al., 2002, Phytotherapy Research. 16 (6): 539-544), and the terpenoid chain of bakuchiol has been reported to be important for its antioxidant activity (Adhikari et al., 2003, Chem Res Toxicol. 16(9):1602-1069). In addition, anti-inflammatory (Nadine Backhouse, C et al., 2001, Journal of Ethnopharmacology. 78 (1): 27-31), anti-bacterial (Katsura, H. et al., 2001, Antimicrobial Agents and Chemotherapy. 45 (11): 3009-3013) and has been reported to exhibit excellent antibacterial effects against oral pathogens (Parimala Devi B et al., 2009, Journal of Pharmacy Research. 2 (11): 1669-1675).

In addition, bakuchiol is a powerful antioxidant and is also known as a substitute for retinol. Bakuchiol's non-irritating antioxidant activates the skin regeneration cycle like retinol, helping to improve wrinkles, promoting collagen production and preventing skin aging ( Chaudhuri RK et al., 2014, International Journal of Cosmetic Science. 36 (3): 221-230). In a clinical study of 44 subjects in 2018, bakuchiol showed a similar level to retinol in terms of improving photoaging, such as wrinkles and pigmentation, but showed better skin tolerance (Dhaliwal, S. et al., 2019, British Journal of Dermatology. 180 (2): 289-296). As such, bakuchiol and retinol have similar mechanisms for enhancing skin elasticity, but retinol reacts sensitively to sunlight, while bakuchiol has high photostability, so it can be used anytime, day or night. In addition, bakuchiol has no skin irritation, so it can be used on all skin types, including dry skin and sensitive skin.

Niacinamide is a type of water-soluble vitamin _B3 known as nicotinamide. It blocks the path of melanin moving to the skin surface to improve skin tone, suppress trouble occurrence, whiten, and improve skin barrier. Together, it promotes the synthesis of cholesterol and fatty acids necessary for the formation of lamellar structure at the same time, reducing the loss of water in the epidermis and improving the amount of lipids. In addition, niacinamide has a hydrotropic property that increases the solubility of water insoluble or poorly soluble substances in water, so it can be used as a cosolubilizing agent to help dissolve poorly soluble substances. .

Ascorbic acid is one of the water-soluble vitamins, also called vitamin C. Vitamin C helps absorption of iron and is involved in biosynthesis and immune function. It is known that deficiency causes scurvy, tissue abnormalities, bone pain, diarrhea, etc., and acts as a collagen synthesis, reducing agent, and antioxidant. Ascorbic acid is contained in green tea, lemon, spinach, cabbage, etc., and most mammals can synthesize ascorbic acid from glucose, but humans cannot synthesize it in the body and must consume it through food. When used as a cosmetic ingredient, a strong antioxidant effect can be expected, and it has a positive effect on the proliferation of collagen fibroblasts, so it has excellent effects on pigmentation, skin elasticity, and anti-aging.

Panthenol is an alcohol derivative of panthenonic acid, also called water-soluble provitamin _B5 . Panthenoic acid is a component of Coenzyme A (CoA), a coenzyme that plays an important role in the metabolism of all cells. When applied to cosmetics, panthenol is known to have no irritation symptoms such as itchiness, stinging, and redness, and has moisturizing, skin protection, inflammation relief, skin regeneration, skin vitality, and skin soothing effects. Panthenol is rapidly absorbed into the mucous membrane, and at this time, panthenol is oxidized to panthenoic acid, which has excellent hygroscopicity and promotes moisture absorption, thereby providing an excellent moisturizing effect. In addition, panthenol acts as a lubricant on the surface of the skin to make the skin soft and smooth, and when applied to physically or chemically damaged hair, it improves the shape and texture of the hair by improving the hair texture, and hair conditioning that makes the hair rich and shiny. has an effect.

Jacaranda flowers are blue-purple flowers of Jacaranda mimosifolia belonging to the genus Jacaranda . It is also called J. acutifolia ). Jacaranda mimosifolia flower extract contains a large amount of polyphenolic compounds and has been reported to have excellent antibacterial and antioxidant effects (Humberto Aguirre-Becerra et al., 2020, Heliyon, 6(12): e05802), Landa mimosifolia leaf extract was analyzed to have antioxidant, antibacterial, anti-inflammatory, and anticancer effects (Rabia Naz, 2020, PLOS ONE, 15(7): e0236319).

Hereinafter, the present invention will be described in detail.

In the present invention, bakuchiol may be chemically synthesized or isolated from plants according to conventional methods in the art.

The plant is a Psoralea genus plant, for example, Psoralea corylifolia L .; Legumes ( Luguminosae );) or Psoralea glandulosa L .; Legumes ( Papilionaceae ). )), or an elm tree ( Ulmus davidiana var. japonica ).

In an exemplary embodiment, the bakuchiol is applied to seeds, stems, barks, twigs, tubers, roots, root barks, young It may be isolated from young shoots, rhizomes, flowers or other reproductive organs, leaves or other aerial parts, or combinations thereof, more preferably from seeds.

In an exemplary embodiment, the bakuchiol is a bogolji ( Psoralea It may be obtained from corylifolia L. ) extract, and the extract may be obtained using water, C ₁ ~ C ₄ lower alcohol, or a mixed solvent thereof, but is not limited thereto.

The bogolji extract is not only the leachate obtained by leaching and exuding from the bogolji, but also the concentrate obtained by concentrating some or all of the leachate again, or the stagnant, pre-, regular, fluid extract and bogolji prepared by drying the concentrate again. It includes both the plant itself as well as the chemicals it contains and exerts its main effect.

Bogolji seed extract used in the present invention can be extracted through a known method.

For example, after drying the bogolji by any method such as natural drying or forced drying, cutting it into small pieces, water, ethanol, butanol, a polar solvent such as acetone; non-polar solvents such as ether, hexane, benzene, chloroform, and ethyl acetate; a mixed solvent of the polar solvent and the non-polar solvent; solvents such as alkaline water; or vegetable oils such as soybean oil and sesame oil; Extracts containing active ingredients can be obtained by leaching by any method such as cooling, filtration, warming, etc.

In an exemplary embodiment, the extract may be suspended in water and sequentially fractionated using n-hexane, ethyl acetate, chloroform, and butanol to obtain each fraction to separate an extract containing bakuchiol.

Bakuchiol is a stable plant-extracted ingredient that does not harm the skin even when used together with other ingredients, and exhibits antioxidant, anti-inflammatory, and antibacterial effects.

The bakuchiol may be preferably a bogolji extract, and may be included in an amount of 0.01 to 13% by weight based on the total weight of the composition, but is not limited thereto. If it is out of the above range, the effect may be insignificant or the feeling of use may be deteriorated.

The niacinamide composition may preferably be included in an amount of 0.01 to 10% by weight based on the total weight, but is not limited thereto, and if it is out of the above range, the effect may be reduced or problems such as deterioration in the feeling of use may occur.

The ascorbic acid composition may preferably contain 0.1 to 15% by weight based on the total weight, but is not limited thereto, and if the composition is out of the above range, the effect and feeling of use may be reduced.

The panthenol, jacaranda flower extract, or a mixture of panthenol and jacaranda flower extract may be included in an amount of 0.01 to 8% by weight based on the total weight of the composition, but is not limited thereto. In addition, the mixing ratio of the mixture of the panthenol and the jacaranda flower extract may be, for example, 1:1, but is not limited thereto.

The ascorbic acid derivative may be Sodium Ascorbyl Phosphate, Magnesium Ascorbyl Phosphate, Ascorbyl Glucoside or Aminopropyl Ascorbyl Phosphate, but is limited thereto It is not, and may preferably be sodium ascorbyl phosphate.

When the ascorbic acid or a derivative thereof is applied to the present invention, it prevents deterioration of elasticity due to skin aging by promoting collagen synthesis, prevents skin aging by inhibiting the action of active oxygen caused by ultraviolet rays, inhibits melanin synthesis and oxidizes It reduces melanin and plays a role in brightening the skin. In addition, the antioxidant function can be exhibited more effectively.

The skin trouble may be selected from the group consisting of atopic dermatitis, pimple, skin rash, rosacea, psoriasis, eczema, seborrheic dermatitis, erythematous disease, allergic pruritus, acne, bullous dermatosis and pemphigus vulgaris. , but is not limited thereto.

On the other hand, the cosmetic composition of the present invention includes ingredients commonly used in cosmetic compositions, in addition to including the above-mentioned ingredients as active ingredients. For example, conventional antioxidants, stabilizers, solubilizers, vitamins, pH adjusters, colorants, flavoring agents, pigments, flavoring agents, bactericides, preservatives, humectants, thickeners, diluents, surfactants, alcohols, neutralizers, softeners, It further includes conventional adjuvants and carriers such as conditioning agents, excipients, inorganic salts, synthetic polymeric materials, and the like.

In addition, their content may be appropriately adjusted according to the use of the final product and its purpose of use.

For example, the carrier includes purified water, monohydric alcohols (ethanol or propyl alcohol), polyhydric alcohols (glycerol, 1,3-butyrene glycol or propylene glycol), higher fatty acids (palmitylic acid or linolenic acid), oils and fats (wheat) Germ oil, camellia oil, jojoba oil, olive oil, squalene, sunflower oil, macadamia peanut oil, avogard oil, or fatty acid glycerides) may be used, but is not particularly limited thereto.

In addition, non-limiting examples of usable surfactants include anionic surfactants, cationic surfactants, nonionic surfactants, amphoteric surfactants, and the like. More specifically, the anionic surfactants include alkylbenzene sulfonates, polyoxyalkylene alkyl sulfate ester salts, alkyl sulfate ester salts, olefin sulfonates, alkyl phosphates, polyoxyalkylene alkyl ether phosphates, and dialkyl sulfosuccines. acid salts, fatty acid salts, and the like, and as nonionic surfactants, polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyhydric alcohol fatty acid partial esters, polyoxyethylene polyhydric alcohol fatty acid partial esters, polyglycerin fatty acid esters, polyoxyethylene Ethylene hydrogenated castor oil derivatives, fatty acid diethanolamide, etc. are mentioned. Examples of cationic surfactants include tertiary aliphatic amine salts, alkyltrimethylammonium halides, and dialkyldimethylammonium halides. Examples of amphoteric surfactants include amide betaine type, imidazolinium betaine type, and sulfobetaine type. etc. can be mentioned.

Examples of the moisturizing agent include glycerin, propylene glycol, 1,3-butylene glycol, dipropylene glycol, sorbitol, and the like, but are not limited thereto.

Examples of the preservative include, but are not limited to, benzoic acid, dehydroacetic acid, paraoxybenzoic acid esters (methyl paraoxybenzoate, butyl paraoxybenzoate, etc.), phenoxyethanol, and the like.

BHA etc. are mentioned as said antioxidant, In addition, a ultraviolet absorber, an anti-inflammatory agent, a cooling agent, etc. can be added.

In addition, the cosmetic composition according to the present invention can be prepared in any formulation commonly prepared in the art, such as lotion, essence, serum, lotion, gel, paste, cream, powder, oil, pack, mist, soap, It may be any one formulation selected from the group consisting of a cleanser, a makeup base, and a foundation. More specifically, softening lotion, nourishing lotion, astringent lotion, toner, lotion, mist, essence, serum, cream, massage cream, pack, makeup base, BB cream, foundation, powder foundation, emulsion foundation, wax foundation, powder , cleansing foam, soap, cleansing cream, cleansing oil, cleansing water or spray formulations.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. may be, but is not limited thereto.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / propellants such as butane or dimethyl ether, but are not limited thereto.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan, but are not limited thereto.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Star cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used, but is not limited thereto.

When the formulation of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide Ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be used, but is not limited thereto.

The cosmetic composition of the present invention can be used every day, and can also be used for an indeterminate period of time. Preferably, the amount, frequency and duration of use can be appropriately adjusted according to the user's age, skin condition or skin type.

Hereinafter, examples are presented to aid understanding of the present invention. However, the following examples are provided to more easily understand the present invention, and the content of the present invention is not limited by the following examples.

Example One. cosmetics composition preparation

A cosmetic composition was prepared with the composition and content (unit: wt%) according to Table 1 below.

Example 1 Example 2 Example 3 Example 4 Comparative Example 1 bakuchiol 0.3 0.3 0.3 0.3 - Niacinamide 0.5 0.5 0.5 0.5 0.5 ascorbic acid 0.4 0.4 0.4 0.4 0.4 panthenol - One - One 0.8 Jacaranda Flower Extract - - 0.5 0.5 0.5

Example 2. Skin vitality enhancement effect test

Normal human fibroblasts were inoculated into each well of a 12-well microplate to form 1×10 ⁶ cells, and cultured in DMEM medium at 37° C. for 24 hours. Thereafter, Examples 1 to 4 and Comparative Example 1 were replaced with serum-free DMEM medium added to a final concentration of 100 μg/ml, followed by additional culture for 48 hours. As a negative control for this, a group replaced with serum-free DMEM medium containing Examples 1 to 4 and Comparative Example 1 was used. After culturing, cells were obtained from each well, and the degree of energy activity in each cell was measured using a cell energy assay kit (ATP assay kit, Amersham, USA).

Skin cell energy activation effect (%) Example 1 80.5 Example 2 85.7 Example 3 81.6 Example 4 91.2 Comparative Example 1 64.4

As shown in Table 2, it can be seen that Examples 1 to 4 have an excellent skin cell energy activity of 80% or more, whereas Comparative Example 1, which does not contain bakuchiol, is significantly lower than Examples 1 to 4. showed activity. On the other hand, among the Examples, Example 4, which contained all of bakuchiol, niacinamide, ascorbic acid, panthenol, and jacaranda flower extract, showed the best skin cell energy activity.

Example 3. Anti-inflammatory effect test

In order to confirm anti-inflammatory and skin trouble improving effects, nitric oxide (NO) production inhibitory ability test was performed by GRIESS method using RAW264.7 cell line (ATCC number: CRL-2278). Specifically, RAW264.7 cells, which are mouse macrophages, were subcultured several times, placed in a 24-well plate so that 3 × 10 ⁵ cells fit into each well, and then cultured for 24 hours. It was then replaced with a cell medium containing the sample at a final concentration of 10 ppm. At this time, L-NMMA (L-NG-Monomethylarginine), an NO-production inhibitor, was treated as a positive control and cultured for 30 minutes, and 1 μg of LPS (Lipopolysaccharide) was treated as a stimulus and cultured for 24 hours. 100 μl of the supernatant was transferred to a 96-well plate, and 100 μl of the GRIESS solution was added and reacted at room temperature for 10 minutes, and then absorbance at 540 nm was measured. The NO generation inhibition rate (%) was calculated using the following formula, and the experiment was performed three times each, and was expressed as an average value.

NO production inhibition rate (%) = {(absorbance of negative control - absorbance of each extract) / absorbance of negative control} × 100

NO production inhibition rate (%) Control (DMSI, 10 ppm) - L-MMA (positive control, 10 ppm) 43.85 Example 1 31.12 Example 2 35.01 Example 3 34.27 Example 4 40.64 Comparative Example 1 25.96

As shown in Table 3, Examples 1 to 3 of the present invention have relatively low activity compared to L-MMA, which is a representative anti-inflammatory drug, but in the case of Example 4, NO production inhibition rate close to that of L-MMA It can be seen that it has an excellent anti-inflammatory effect. On the other hand, Comparative Example 1 showed much lower activity than L-MMA.

Example 4. Skin moisturizing effect test

Aquaporins 3 (AQP3) is a protein that regulates the movement of water and glycerol between cells, and it has been reported that problems occur in the process of maintaining water in the epidermal layer in animal models of mice in which AQP3 gene expression is suppressed. there is a bar AQP3 expression decreases with age, and the decrease in AQP3 expression level is known to be one of the causes of skin dryness. Accordingly, the skin moisturizing effect was confirmed by checking the expression level of AQP3.

First, HEKn (Human Epidermal Keratinocyte neonatal, C-001-5C, Invitrogen, USA) cells, which are keratinocytes, were cultured in DMEM medium supplemented with 10% Fetal Bovine Serum (FBS), penicillin, and streptomycin. Keratinocytes were treated with Example 4 at a concentration of 1 to 10,000 ppm (w/w) for 12 hours, and total RNA was isolated using TRIzol™ (Invitrogen, USA). RNA concentration was measured spectrophotometrically, and RNA integrity was measured using BioAnalyzer 2100 (Agilent Technologies, USA). 4 μg of RNA was reverse transcribed into cDNA using SuperScript®III reverse transcriptase (Invitrogen, USA) and aliquots were stored at -70°C, and the expression level of the target gene was measured by quantitative real-time TaqMan RT-PCR (7500Fast, Applied Biosystems, USA). Cycle conditions were 95°C for 10 minutes, 50 cycles at 95°C for 15 minutes, and 60°C for 1 minute. Through this, the relative value of how much the gene expression increased compared to the gene expression of the control group was measured. The AQP3 gene expression level was corrected based on the mRNA expression level of RPLPO for the analysis of the relative expression pattern.

As a result, as shown in Figure 1, it was confirmed that the cosmetic composition of the present invention has a skin moisturizing effect by increasing AQP3 gene expression in keratinocytes in a concentration-dependent manner. As described above, by maintaining sufficient moisture in the skin, it is possible to prevent skin drying and damage to the stratum corneum, thereby helping to alleviate skin troubles.

Example 5. Whitening effect test

Examples 1 to 4 and Comparative Example 1 were added to the culture medium of B-16 mouse melanoma cells to test the whitening effect at the cellular level (Lotan R., Lotan D. 1980, Cancer Res. 40: 3345-3350). The final concentration of the sample was 50 μg/ml, and was added to the B-16 rat melanoma cell culture medium together with an aqueous solution of arbutin, and cultured for 3 days. The cells were treated with trypsin, detached from the culture vessel, centrifuged, and then melanin was extracted. Here, 1 ml of sodium hydroxide solution (1N concentration) was added and heated for 10 minutes to dissolve melanin, and absorbance was measured at 400 nm using a spectrophotometer. The amount of melanin produced was expressed as an absorbance per unit cell number (10 ⁶ cell). The relative amount of melanin production in the group treated with the sample relative to the control group not treated with the sample was calculated as an inhibition rate (%), and the results are shown in Table 4 below.

Inhibition rate (%) Control (no additives) - Arbutin (300 μg/ml) 67.2 Example 1 51.6 Example 2 54.6 Example 3 58.4 Example 4 66.7 Comparative Example 1 43.1

As can be seen in Table 4, Example 4 shows an excellent whitening effect by confirming that it exhibits a similar inhibition rate compared to arbutin, a known whitening substance, thereby giving vitality to the skin and increasing skin vitality. can

Example 6. Skin irritation assessment

For skin irritation evaluation for Example 4, 20 adult female subjects aged 30 to 50 years old were asked to indicate whether or not they had skin irritation through a questionnaire, and visual evaluation was conducted. The questionnaire for skin irritation evaluation consisted of a total of 8 items: erythema, edema, scaling, itching, stinging, burning, stiffness and tingling. As a result of the survey, it was confirmed that no adverse skin reactions were observed in all subjects, and no adverse skin reactions were observed in the visual evaluation.

The cosmetic composition of the present invention composed as described above contains bakuchiol, niacinamide, and ascorbic acid as active ingredients, thereby improving skin inflammation through antibacterial activity, soothing the skin, and providing vitality to the skin. have In addition, it can be seen that by further including panthenol and jacaranda flower extract, a more excellent effect is exerted due to the synergistic effect between the components.

Formulation examples of the composition according to one aspect of the present disclosure are described below, but may be applied to various other formulations, which are only intended to be specifically described, not intended to limit the present disclosure.

formulation example 1. Lotion

A lotion was prepared in a conventional manner according to the composition described below. (weight%)

Bakuchiol 0.3

Niacinamide 0.5

Ascorbic acid 0.4

Panthenol 1

Jacaranda Flower Extract 0.5

Glycerin 3.0

Butylene glycol 3.0

Propylene glycol 3.0

Carboxyvinyl polymer 0.1

Beeswax 4.0

Polysorbate 60

Caprylic/Capric Triglyceride 5.0

Squalane 5.0

Sorbitan sesquioleate 1.5

Cetearyl Alcohol 1.0

Tromethamine 0.2

Appropriate amount of preservatives and flavorings

Remaining amount of purified water

formulation example 2. Lotion

A lotion was prepared in a conventional manner according to the composition described below. (weight%)

Bakuchiol 0.7

Niacinamide 0.7

Ascorbic acid 0.8

Panthenol 1.0

Jacaranda Flower Extract 1.0

Diethyl Ascorbic Acid 1.0

sodium citrate 0.1

Licorice extract 0.2

1,3-butylene glycol 3.0

Remaining amount of purified water

formulation example 3. Cream

A cream was prepared in a conventional manner according to the composition described below. (weight%)

Bakuchiol 0.6

Niacinamide 0.8

Ascorbic acid 0.7

Panthenol 1.0

Jacaranda Flower Extract 0.6

Glycerin 3.5

Butylene glycol 3.0

Liquid Paraffin 7.0

Beta Glucan 7.0

Carbomer 0.1

Caprylic/Capric Triglyceride 3.0

Squalane 5.0

Cetearyl Glucoside 1.5

Sorbitan Stearate 0.4

Polysorbate 60 1.2

Tromethamine 0.1

Appropriate amount of preservatives and flavorings

Remaining amount of purified water

The above description is merely illustrative of the present invention, and those skilled in the art will be able to make various modifications without departing from the essential characteristics of the present invention. Therefore, the embodiments disclosed in this specification are intended to explain, not limit, the present invention, and the spirit and scope of the present invention are not limited by these embodiments. The protection scope of the present invention should be construed according to the following claims, and all technologies within the equivalent range should be construed as being included in the scope of the present invention.

Claims (10)

bakuchiol; niacinamide; ascorbic acid; panthenol; And a cosmetic composition for enhancing skin vitality and improving skin troubles, comprising a jacaranda flower extract as an active ingredient.
delete delete The cosmetic composition according to claim 1, wherein the bakuchiol is a bogolji extract.
The method of claim 1, wherein the skin trouble consists of atopic dermatitis, pimple, skin rash, rosacea, psoriasis, eczema, seborrheic dermatitis, erythematous disease, allergic pruritus, acne, bullous dermatosis and pemphigus vulgaris. A cosmetic composition characterized in that selected from the group
The cosmetic composition according to claim 1, wherein the bakuchiol is present in an amount of 0.01 to 13% by weight based on the total weight of the composition.
The cosmetic composition according to claim 1, wherein the niacinamide is present in an amount of 0.01 to 10% by weight based on the total weight of the composition.
The cosmetic composition according to claim 1, wherein the ascorbic acid is present in an amount of 0.1 to 15% by weight based on the total weight of the composition.
The cosmetic composition according to claim 1, wherein the panthenol or jacaranda flower extract is present in an amount of 0.01 to 8% by weight based on the total weight of the composition.
The method of claim 1, wherein the composition is formulated in any one selected from the group consisting of lotion, essence, serum, lotion, gel, paste, cream, powder, oil, pack, mist, soap, cleanser, makeup base, and foundation Cosmetic composition characterized in that