KR102568325B1 - Method of cultivating Brassica rapa for increasing the amount of glucosinolate - Google Patents

Abstract

The present invention provides a method for growing cruciferous vegetables with increased glucosinolate content, including the step of applying stress to the plant during the cultivation process of cruciferous vegetables, and cruciferous vegetables and cruciferous vegetables with increased glucosinolate content grown by the method It relates to a method for increasing the content of glucosinolates in cruciferous vegetables, comprising the step of applying stress to the plant during the growing process of the vegetable. Using the method for growing cruciferous vegetables provided in the present invention, it is possible to grow cruciferous vegetables containing a high content of glucosinolates exhibiting various pharmacological activities such as anti-inflammatory activity, antibacterial activity, and detoxifying activity. Or it will be widely used in the development of raw materials for functional food compositions.

Description

Cultivation method of cruciferous vegetables with increased glucosinolate content {Method of cultivating Brassica rapa for increasing the amount of glucosinolate}

The present invention relates to a method for growing cruciferous vegetables with increased glucosinolate content. Method for growing cruciferous vegetables with increased glucosinolate content, cruciferous vegetables with increased glucosinolate content grown by the method and glucosinol contained in cruciferous vegetables, including applying stress to plants during the cultivation process of the cruciferous vegetables It relates to a method of increasing the content of the rate.

Cruciferous plants, which are characterized by having flowers with four petals in the shape of a cross, include commonly available edible vegetables such as radish, Chinese cabbage, cabbage, broccoli, and bok choy. These cruciferous vegetables are known to contain glucosinolates that exhibit strong anticancer activity effective in the treatment of bladder cancer, breast cancer, and liver cancer. The glucosinolate is known to exhibit various pharmacological activities such as anti-inflammatory activity, antibacterial activity, and detoxification activity in addition to anticancer activity, so cruciferous vegetables containing the glucosinolate can be used as a raw material for safe and effective functional foods. indicates the possibility of However, since the content of glucosinolates contained in the cruciferous vegetables is at a very low level, general cruciferous vegetables have a disadvantage in that they are somewhat insufficient to be used as a raw material for functional foods.

In order to solve these disadvantages, various studies have been conducted. For example, Korean Patent Registration No. 1525143 discloses a method of increasing the content of glucosinolate by treating radish sprouts with jasmonic acid or methyl jasmonate. has been initiated.

On the other hand, when a stress is applied by creating an abnormal environment during plant cultivation, the level of various components may be increased while resistance to stress is imparted to the cultivated plant. For example, Korean Patent Publication No. 10-2009-0036192 discloses a method of increasing the content of resveratrol contained in peanut sprouts by applying periodic temperature stress during cultivation of peanut sprouts. No. 0991246 discloses a method of cultivating sprout crops with increased antioxidant activity by cultivating sprout crops under oxidative stress cultivation conditions, and Korean Patent Registration No. 10-2113804 discloses a method of cultivating plants under conditions of applying sound wave stress to improve the quality of plants. A method for promoting growth and preventing pest damage is disclosed. However, a method of increasing the content of glucosinolate by applying stress has not yet been reported.

Under this background, in order to develop a method that can effectively increase the content of glucosinolate contained in cruciferous vegetables, as a result of intensive research efforts, when drying stress or light stress is applied during the cultivation of cruciferous vegetables, the cultivation Through this, it was confirmed that cruciferous vegetables with increased glucosinolate content could be produced, and the present invention was completed.

The main object of the present invention is to provide a method for growing cruciferous vegetables with increased glucosinolate content.

Another object of the present invention is to provide cruciferous vegetables with increased glucosinolate content grown by the above method.

Another object of the present invention is to provide a method for increasing the content of glucosinolates in cruciferous vegetables.

One embodiment of the present invention for achieving the above object provides a method for growing cruciferous vegetables with increased glucosinolate content, comprising applying stress to the plant during the growing process of the cruciferous vegetables.

The term "cruciferous vegetables" of the present invention refers to vegetables belonging to the dicotyledonous plant Papaveraceae, and is also called cabbage vegetables or mustard vegetables. Most of them are herbaceous plants, the leaves have a tangy taste, the leaves are single leaves or compound leaves, there are no stipules, the flowers are bisexual, and there are 4 calyx pieces. In the case of a flower, there are 6 stamens, and the inner 4 make up a long quatrain. The ovary has two chambers in appearance. The fruit is a pod, and the seed has no endosperm.

In the present invention, the cruciferous vegetables are not particularly limited thereto as long as the content of glucosinolate is increased by the method of the present invention, but for example, bok choy, cabbage, kale, broccoli, mustard, rapeseed, kohlrabi , Homu, turnip, red iris, Chinese cabbage, Komatsuna, chaesim, mizuna, etc., and as another example, it can be bok choy.

Cultivation of the cruciferous vegetables can be carried out by the hydroponic cultivation method as well as the conventional open-field cultivation. When grown by the hydroponic cultivation method, in order to improve cultivation efficiency and effectively apply light stress, artificial light-type smart farm can do.

The stress applied to the cruciferous vegetables is used to create an abnormal cultivation environment, and these abnormal environmental conditions impart environmental resistance to the plant, and this environmental resistance may cause overexpression of a specific component in the plant. The stress is not particularly limited thereto, but as an example, drying stress, light stress, etc. may be used alone or in combination. Such stress may be applied once or repeatedly during the cultivation of cruciferous vegetables. .

For example, drying stress can be applied by exposing the roots to the air for 8 to 16 hours and ventilating them to dry the roots when growing cruciferous vegetables. It may additionally include a step of recovering by immersion during drying, and the act of recovering after such drying stress may be applied only once during the cultivation process, or may be repeatedly applied once a week for a certain period of time.

In addition, light stress can be applied by irradiating light for 16-24 hours a day from 10-20 days after sowing seeds of cruciferous vegetables. Such light stress may be applied only once during the cultivation process, and for a certain period of time It may be applied repeatedly once a day. At this time, the wavelength of the irradiated light is not particularly limited thereto, but may be, for example, 445-470 nm, 650-665 nm, etc., and, as another example, may be a warm white complex wavelength. For example, after irradiating light of 445-470 nm or 650-665 nm, light having a warm white series complex wavelength may be additionally irradiated. In addition, the irradiation amount of the light is also not particularly limited thereto, but as an example, it may be 120 ± 5 μmol/m ² s.

In the present invention, when drying stress or light stress is applied during the cultivation of the cruciferous vegetables, an effect of increasing the level of glucosinolate can be obtained in the cruciferous vegetables harvested through the cultivation.

The term "glucosinolate" of the present invention is a type of organic compound containing sulfur, which not only exhibits strong anticancer activity in bladder cancer, breast cancer, and liver cancer, but also has various activities such as anti-inflammatory activity, antibacterial activity, and detoxifying activity. It is known to exhibit pharmacological activity. The glucosinolate is known to be included in cruciferous vegetables, and when ingested, it can be converted to isothiocyanate by the hydrolytic enzyme myrosinase, and based on the chemical structure, aliphatic ) glucosinolate, indolic glunosinolate, aromatic glucosinolate, etc. As an example, glucoiberin, progoitrin, glucoraphanin, sinigrin, gluconapin, glucobrassicin, gluconasturtiin, neoglucobrassicin, etc. can include

According to one embodiment of the present invention, drying stress and light stress were provided individually or in combination while growing bok choy, a kind of cruciferous vegetable, by a hydroponic cultivation method. After the cultivation was completed, the levels of total glucosinolate, aliphatic glucosinolate, indoline glucosinolate, and aromatic glucosinolate contained in each cultivated bok choy were measured, and the type of stress provided Although there were variations depending on the concentration, it was confirmed that the levels of various glucosinolates were increased in bok choy grown under stress (FIGS. 2a to 2d and 3a to 3e).

Another embodiment of the present invention provides a method for increasing the content of glucosinolates in cruciferous vegetables, comprising the step of applying stress to a plant during the growing process of the cruciferous vegetables.

The cruciferous vegetables, stress, glucosinolate, etc. are the same as defined above.

As described above, since glucosinolate is a component already contained in wild-type cruciferous vegetables at a certain level and exhibits various pharmacological activities, cruciferous vegetables containing a large amount of glucosinolate can be used as a raw material for functional foods.

From this point of view, the method of increasing the content of glucosinolate in cruciferous vegetables provided in the present invention can produce cruciferous vegetables containing a higher content of glucosinolates than conventional cruciferous vegetables, so that more effective It can be used in the production of raw materials for functional foods.

Another embodiment of the present invention provides a cruciferous vegetable with enhanced glucosinolate content prepared by the above method.

The cruciferous vegetables with increased glucosinolate content provided in the present invention contain a level of glucosinolate that is at least 20% higher than that of common cruciferous vegetables.

According to one embodiment of the present invention, bok choy grown while applying drying stress and light stress individually or in combination has a 30 to 60% higher level of total glucosinolate than the total glucosinolate content contained in general bok choy. Contains oleate (Fig. 2a), contains 100 to 130% higher level of aliphatic glucosinolate than the content of aliphatic glucosinolate contained in common bok choy (Fig. 2b), included in common bok choy It contains indoline glucosinolate at a level that is 25 to 90% higher than the indoleic glucosinolate content of bok choy (FIG. 2c), and is 87 to 350% higher than the aromatic glucosinolate content contained in general bok choy. levels of aromatic glucosinolates (Fig. 2d).

Using the method for growing cruciferous vegetables provided in the present invention, it is possible to grow cruciferous vegetables containing a high content of glucosinolates exhibiting various pharmacological activities such as anti-inflammatory activity, antibacterial activity, and detoxifying activity. Or it will be widely used in the development of raw materials for functional food compositions.

Figure 1a is a graph showing the results of comparing the changes in fresh weight measured from 4 to 6 weeks for control (CON) and stress groups (LD, WS and LD + WS) bok choy over the course of the cultivation period of bok choy. am.
Figure 1b is a graph showing the results of comparing the change in dry weight measured from 4 to 6 weeks for control (CON) and stress groups (LD, WS, and LD + WS) bok choy over the course of the cultivation period of bok choy. am.
Figure 1c is a graph showing the results of comparing the changes in plant length measured from 4 to 6 weeks for control (CON) and stress groups (LD, WS and LD + WS) bok choy over the course of the cultivation period of bok choy. am.
Figure 1d is a graph showing the results of comparing changes in the number of leaves measured from 4 to 6 weeks for control (CON) and stress groups (LD, WS and LD + WS) bok choy over the course of the cultivation period of bok choy. .
Figure 2a shows the results of comparing the level change of total GLS measured from 4 to 6 weeks for control (CON) and stress groups (LD, WS and LD + WS) bok choy over the course of the cultivation period of bok choy. it's a graph
Figure 2b shows the results of comparing the level change of aliphatic GLS measured from 4 to 6 weeks for control (CON) and stress groups (LD, WS and LD + WS) bok choy over the course of the cultivation period of bok choy. it's a graph
Figure 2c shows the results of comparing the level change of indolic GLS measured from 4 to 6 weeks for control (CON) and stress groups (LD, WS and LD + WS) bok choy over the course of the cultivation period of bok choy. it's a graph
Figure 2d is a comparison of the level change of aromatic GLS measured from 4 to 6 weeks for control (CON) and stress group (LD, WS and LD + WS) bok choy according to the lapse of the cultivation period of bok choy. Representing the result it's a graph
Figure 3a compares the level change of total GLS measured from 4 to 6 weeks for one plant of control group (CON) and stress group (LD, WS and LD + WS) according to the cultivation period of bok choy. A graph showing the result.
Figure 3b compares the level change of aliphatic GLS measured from 4 to 6 weeks for one plant of control (CON) and stress groups (LD, WS and LD + WS) bok choy over the course of the cultivation period of bok choy. A graph showing the result.
Figure 3c compares the level change of indolic GLS measured from 4 to 6 weeks for one plant of control (CON) and stress groups (LD, WS and LD + WS) according to the cultivation period of bok choy. A graph showing the result.
Figure 3d compares the level change of aromatic GLS measured from 4 to 6 weeks for one plant of control (CON) and stress group (LD, WS and LD + WS) bok choy over the course of the cultivation period of bok choy. A graph showing the result.
Figure 3e compares the level change of glucoraphanin measured from 4 to 6 weeks for one plant of control group (CON) and stress group (LD, WS and LD + WS) according to the cultivation period of bok choy. is a graph that represents

Hereinafter, the present invention will be described in more detail through examples. However, these examples are intended to illustrate the present invention by way of example, and the scope of the present invention is not limited to these examples.

Example 1: Cultivation of bok choy

Bok choy seeds were sown on a rock wool medium, and the medium was saturated with water to induce water penetration into the seed coat to artificially germinate. After germination, through the seedling process of supplying light, moisture, and carbon dioxide, it was cultivated until the number of true leaves reached four while performing environmental control for day and night temperature change and irrigation until the number of true leaves reached two. Then, the cultivated bok choy sprouts were cultivated through the DFT hydroponic cultivation system. The wavelengths of light irradiated during cultivation were 445-470 nm and 650-665 nm as the central wavelengths, and additional warm white series complex wavelengths were used. was set to 120 ± 5 μmol/m2s, the cultivation temperature was set to 23 ° C during the day and 18 ° C at night, the concentration of carbon dioxide in the atmosphere was set to 800 ppm during the day, and Otsuka culture medium (EC1.2) was used as the culture medium. .

Example 2: Cultivation and analysis of stressed bok choy

Example 2-1: Cultivation of stressed bok choy

In Example 1, after performing the seedling process until the number of true leaves is 4 to 8, the cultivated bok choy shoots were grown through the NFT hydroponic cultivation system, and experimental groups to which stress conditions were added were obtained, respectively.

Roughly, bok choy (drying) treated two or more times before harvesting the step of removing the culture medium, drying the underground part under a constant temperature condition of 13-23 ° C for 8-16 hours, and then recovering the culture medium under a constant temperature condition of 13-23 ° C. stress, WS), bok choy grown in an environment of 16-24 hours per day from 10-20 days after sowing (light stress, LD) and cultivated in an environment of 16-24 hours per day from 10-20 days after sowing, Bok choy (combined stress, LD+WS), which was subjected to ventilation drying stress and recovery stages in the basement, were cultivated, respectively. At this time, bok choy grown without stress was used as a control group.

Example 2-2: Growth index analysis

Growth indices (fresh weight, dry weight, plant height and number of leaves) of bok choy grown in the control group (CON) and stress groups (LD, WS and LD+WS) in Example 2-1 were analyzed (FIGS. 1a to 1d).

Figure 1a is a graph showing the results of comparing the changes in fresh weight measured from 4 to 6 weeks for control (CON) and stress groups (LD, WS and LD + WS) bok choy over the course of the cultivation period of bok choy. am.

As shown in Figure 1a, in the 6th week, compared to the fresh weight of the control group, the fresh weight of WS bok choy decreased to about 40%, the fresh weight of LD bok choy increased to about 150%, and the raw weight of LD + WS bok choy It was confirmed that the weight was reduced to about 98%.

Figure 1b is a graph showing the results of comparing the change in dry weight measured from 4 to 6 weeks for control (CON) and stress groups (LD, WS, and LD + WS) bok choy over the course of the cultivation period of bok choy. am.

As shown in Figure 1b, in the 6th week, compared to the dry weight of the control group, the dry weight of WS bok choy decreased to about 60%, the dry weight of LD bok choy increased to about 200%, and the dry weight of LD + WS bok choy It was confirmed that the weight increased to about 118%.

Figure 1c is a graph showing the results of comparing the changes in plant length measured from 4 to 6 weeks for control (CON) and stress groups (LD, WS and LD + WS) bok choy over the course of the cultivation period of bok choy. am.

As shown in FIG. 1c, at week 6, compared to the control group, the plant length of WS bok choy decreased to about 79%, the plant length of LD bok choy increased to about 120%, and the plant length of LD + WS bok choy was about 94 It was confirmed that it was reduced to the % level.

Figure 1d is a graph showing the results of comparing changes in the number of leaves measured from 4 to 6 weeks for control (CON) and stress groups (LD, WS and LD + WS) bok choy over the course of the cultivation period of bok choy. .

As shown in FIG. 1d, at week 6, compared to the number of leaves in the control group, the plant length of WS bok choy decreased to about 90%, the number of leaves of LD bok choy increased to about 115%, and the number of leaves of LD + WS bok choy was about 109 It was confirmed that it increased to the % level.

Example 2-3: GLS (glucosinolate) level analysis

The levels of various GLS (total GLS, aliphatic GLS, indolic GLS and aromatic GLS) contained in bok choy of the control group (CON) and stress group (LD, WS and LD + WS) grown in Example 2-1 were analyzed ( Figures 2a to 2d).

The level of the GLS was analyzed as follows: 100 mg of dried bok choy powder was put in 2 mL of 70% ethanol and extracted at 90° C. for 10 minutes to obtain a stock extract. After adding 1 mM glucotropaeolin to the stock extract and titrating the total volume to 4 mL, 0.1 mL of 1 M lead acetate and 0.1 mL of 1 M barium acetate were added and mixed to 1 mL of the extract, respectively, and centrifuged (10,000 g , 5 min) to obtain the supernatant. 1 mL of the obtained supernatant was added to a column filled with DEAE (diethyl-aminoethyl) sephadex A-25 anion exchange resin, and reacted at room temperature for 18 hours to obtain an extract from which sulfur components were removed. 0.5 mL of distilled water was added to the extract from which the sulfur component was removed to dissolve it, and a sample was prepared by removing impurities using a 0.2 μm filter. Using the prepared sample, reverse-phase high-performance liquid chromatography analysis using an ODS-AQ (150 mm × 4.6 mm, 5 μm) column was performed. The reverse-phase high-performance liquid chromatography analysis was performed using a flow rate (1 mL / min), a concentration gradient using solution A (acetonitrile) and solution B (distilled water) (0 min, initial condition was 0.5: 99.5 (A: B); 7 min, 1.5:98.5; 15 min, 10:90; 25 min, 20:80; 26 min, 30:70, gradient held until 29 min; 31 min, 0.5:99.5; 35 min, 0.5:99.5) conditions, The final product was analyzed at 227 nm, and the content of each GLS was calculated by comparison with the standard material of each GLS.

Figure 2a shows the results of comparing the level change of total GLS measured from 4 to 6 weeks for control (CON) and stress groups (LD, WS and LD + WS) bok choy over the course of the cultivation period of bok choy. it's a graph

As shown in Figure 2a, at week 6, compared to the total GLS level of the control group, the total GLS level of WS bok choy increased to about 130%, and the total GLS level of LD bok choy increased to about 160%, LD + WS It was confirmed that the total GLS level of bok choy increased to about 138%.

Figure 2b shows the results of comparing the level change of aliphatic GLS measured from 4 to 6 weeks for control (CON) and stress groups (LD, WS and LD + WS) bok choy over the course of the cultivation period of bok choy. it's a graph

As shown in Figure 2b, at week 6, compared to the aliphatic GLS level of the control group, the aliphatic GLS level of WS bok choy increased to about 200%, and the aliphatic GLS level of LD bok choy increased to about 230%, LD + WS It was confirmed that the aliphatic GLS level of bok choy was increased to about 206%.

Figure 2c shows the results of comparing the level change of indolic GLS measured from 4 to 6 weeks for control (CON) and stress groups (LD, WS and LD + WS) bok choy over the course of the cultivation period of bok choy. it's a graph

As shown in Figure 2c, at week 6, compared to the indolic GLS level of the control group, the indolic GLS level of WS bok choy increased to about 150%, and the indolic GLS level of LD bok choy increased to about 190%, LD + WS It was confirmed that the indolic GLS level of bok choy was increased to about 125%.

Figure 2d is a comparison of the level change of aromatic GLS measured from 4 to 6 weeks for control (CON) and stress group (LD, WS and LD + WS) bok choy according to the lapse of the cultivation period of bok choy. Representing the result it's a graph

As shown in Figure 2d, at week 6, compared to the aromatic GLS level of the control group, the aromatic GLS level of WS bok choy increased to about 187%, and the aromatic GLS level of LD bok choy increased to about 450%, LD + WS It was confirmed that the aromatic GLS level of bok choy increased to about 219%.

Example 2-4: Level analysis of GLS (glucosinolate) per plant

Various GLS (total GLS, aliphatic GLS, indolic GLS and aromatic GLS) contained in each one plant of the control group (CON) and stress group (LD, WS and LD + WS) bok choy grown in Example 2-1 levels were analyzed (Figs. 3A-3D).

Figure 3a compares the level change of total GLS measured from 4 to 6 weeks for one plant of control group (CON) and stress group (LD, WS and LD + WS) according to the cultivation period of bok choy. A graph showing the result.

As shown in Figure 3a, at week 6, compared to the total GLS level of the control group, the total GLS level of WS bok choy decreased to about 81%, and the total GLS level of LD bok choy increased to about 240%, LD + WS It was confirmed that the total GLS level of bok choy increased to about 140%.

Figure 3b compares the level change of aliphatic GLS measured from 4 to 6 weeks for one plant of control (CON) and stress groups (LD, WS and LD + WS) bok choy over the course of the cultivation period of bok choy. A graph showing the result.

As shown in Figure 3b, at week 6, compared to the aliphatic GLS level of the control group, the aliphatic GLS level of WS bok choy decreased to about 80%, and the aliphatic GLS level of LD bok choy increased to about 230%, LD + WS It was confirmed that the aliphatic GLS level of bok choy was increased to about 140%.

Figure 3c compares the level change of indolic GLS measured from 4 to 6 weeks for one plant of control (CON) and stress groups (LD, WS and LD + WS) according to the cultivation period of bok choy. A graph showing the result.

As shown in Figure 3c, at week 6, compared to the indolic GLS level of the control group, the indolic GLS level of WS bok choy decreased to about 96%, and the indolic GLS level of LD bok choy increased to about 290%, LD + WS It was confirmed that the indolic GLS level of bok choy increased to about 134%.

Figure 3d compares the level change of aromatic GLS measured from 4 to 6 weeks for one plant of control (CON) and stress group (LD, WS and LD + WS) bok choy over the course of the cultivation period of bok choy. A graph showing the result.

As shown in Figure 3d, at week 6, compared to the aromatic GLS level of the control group, the aromatic GLS level of WS bok choy increased to about 109%, and the aromatic GLS level of LD bok choy increased to about 642%, LD + WS It was confirmed that the aromatic GLS level of bok choy increased to about 234%.

Figure 3e compares the level change of glucoraphanin measured from 4 to 6 weeks for one plant of control group (CON) and stress group (LD, WS and LD + WS) according to the cultivation period of bok choy. is a graph that represents

As shown in Figure 3e, at week 5, compared to the control group's glucoraphanin level, the glucoraphanin level of WS bok choy increased to about 778%, and the glucoraphanin level of LD bok choy decreased to about 67%. It was confirmed that the level increased to about 188%.

Summarizing the results of FIGS. 2a to 2d and 3a to 3e, when dry stress or light stress is applied during cultivation of bok choy, various GLS levels are increased in bok choy, and in particular, when light stress is applied, various GLS levels are the highest in bok choy. level was found to be indicated.

In addition, it was confirmed that changing the level of specific GLS was different depending on the type of stress, and especially when drying stress was applied, it was found that the level of glucoraphanin could be increased in bok choy.

In addition, it was found that it is effective to apply the stress several times during the cultivation period, rather than applying the stress once immediately before harvest.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be embodied in other specific forms without changing its technical spirit or essential features. In this regard, the embodiments described above should be understood as illustrative in all respects and not limiting. The scope of the present invention should be construed as including all changes or modifications derived from the meaning and scope of the claims to be described later and equivalent concepts rather than the detailed description above are included in the scope of the present invention.

Claims (10)

Light stress to plants during cultivation of bok choy; Or light stress and dry stress; A method for growing bok choy in which the glucosinolate content is increased by 20% or more compared to the content contained in bok choy grown without applying stress, comprising the step of applying light stress and drying stress,
The light stress is irradiated with light of 445-470 nm or 650-665 nm for 16-24 hours a day from 10-20 days after sowing seeds of bok choy, and then additionally irradiating light having a complex wavelength of warm white (warm white) is carried out in a way
The drying stress is a cultivation method in which the roots are exposed to the air for 8 to 16 hours during cultivation of bok choy and ventilated to dry the roots.
delete According to claim 1,
The cultivation method is carried out in a hydroponic cultivation method in an artificial light type smart farm.
delete delete delete According to claim 1,
After drying the roots, further comprising the step of recovering the roots by immersing them in a culture medium for 18 to 36 hours, the cultivation method.
Light stress to plants during cultivation of bok choy; Or light stress and dry stress; as a method of increasing the content of glucosinolate (glucosinolate) by 20% or more compared to the content contained in bok choy grown without applying stress, including the step of applying,
The light stress is irradiated with light of 445-470 nm or 650-665 nm for 16-24 hours a day from 10-20 days after sowing seeds of bok choy, and then additionally irradiating light having a complex wavelength of warm white (warm white) is carried out in a way
The drying stress is a method in which the roots are exposed to the air for 8 to 16 hours during cultivation of bok choy and ventilated to dry the roots.
A bok choy cultivated by the method of any one of claims 1, 3, and 7 and having an increased glucosinolate content,
The content of glucosinolate contained in the bok choy is increased by 20% or more compared to the content of glucosinolate contained in the bok choy grown without applying stress. delete