KR102561368B1 - 실질적으로 코아굴로겐이 없는 리물루스 아메보사이트 라이세이트를 이용한 내독소 검출방법 - Google Patents
실질적으로 코아굴로겐이 없는 리물루스 아메보사이트 라이세이트를 이용한 내독소 검출방법 Download PDFInfo
- Publication number
- KR102561368B1 KR102561368B1 KR1020197005917A KR20197005917A KR102561368B1 KR 102561368 B1 KR102561368 B1 KR 102561368B1 KR 1020197005917 A KR1020197005917 A KR 1020197005917A KR 20197005917 A KR20197005917 A KR 20197005917A KR 102561368 B1 KR102561368 B1 KR 102561368B1
- Authority
- KR
- South Korea
- Prior art keywords
- lal
- coagulogen
- delete delete
- endotoxin
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/579—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving limulus lysate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/10—Anilides
- C12Q2337/12—Para-Nitroanilides p-NA
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
- G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
- G01N2400/50—Lipopolysaccharides; LPS
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Water Supply & Treatment (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662370493P | 2016-08-03 | 2016-08-03 | |
| US62/370,493 | 2016-08-03 | ||
| PCT/US2017/045137 WO2018026941A1 (en) | 2016-08-03 | 2017-08-02 | Method of detecting an endotoxin using limulus amebocyte lysate substantially free of coagulogen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20190037284A KR20190037284A (ko) | 2019-04-05 |
| KR102561368B1 true KR102561368B1 (ko) | 2023-08-01 |
Family
ID=61069187
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020197005917A Active KR102561368B1 (ko) | 2016-08-03 | 2017-08-02 | 실질적으로 코아굴로겐이 없는 리물루스 아메보사이트 라이세이트를 이용한 내독소 검출방법 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US11156614B2 (enExample) |
| EP (2) | EP3494226B1 (enExample) |
| JP (1) | JP7044222B2 (enExample) |
| KR (1) | KR102561368B1 (enExample) |
| CN (1) | CN109790561B (enExample) |
| IL (1) | IL264464B2 (enExample) |
| SG (1) | SG11201900767VA (enExample) |
| WO (1) | WO2018026941A1 (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10704021B2 (en) | 2012-03-15 | 2020-07-07 | Flodesign Sonics, Inc. | Acoustic perfusion devices |
| US9458450B2 (en) | 2012-03-15 | 2016-10-04 | Flodesign Sonics, Inc. | Acoustophoretic separation technology using multi-dimensional standing waves |
| US9950282B2 (en) | 2012-03-15 | 2018-04-24 | Flodesign Sonics, Inc. | Electronic configuration and control for acoustic standing wave generation |
| US10967298B2 (en) | 2012-03-15 | 2021-04-06 | Flodesign Sonics, Inc. | Driver and control for variable impedence load |
| CN105939767B (zh) | 2014-01-08 | 2018-04-06 | 弗洛设计声能学公司 | 具有双声电泳腔的声电泳装置 |
| US11377651B2 (en) | 2016-10-19 | 2022-07-05 | Flodesign Sonics, Inc. | Cell therapy processes utilizing acoustophoresis |
| US11021699B2 (en) | 2015-04-29 | 2021-06-01 | FioDesign Sonics, Inc. | Separation using angled acoustic waves |
| US11708572B2 (en) | 2015-04-29 | 2023-07-25 | Flodesign Sonics, Inc. | Acoustic cell separation techniques and processes |
| US11474085B2 (en) | 2015-07-28 | 2022-10-18 | Flodesign Sonics, Inc. | Expanded bed affinity selection |
| US11459540B2 (en) | 2015-07-28 | 2022-10-04 | Flodesign Sonics, Inc. | Expanded bed affinity selection |
| US11085035B2 (en) | 2016-05-03 | 2021-08-10 | Flodesign Sonics, Inc. | Therapeutic cell washing, concentration, and separation utilizing acoustophoresis |
| US11214789B2 (en) | 2016-05-03 | 2022-01-04 | Flodesign Sonics, Inc. | Concentration and washing of particles with acoustics |
| KR102561368B1 (ko) | 2016-08-03 | 2023-08-01 | 론자 워커스빌 아이엔씨. | 실질적으로 코아굴로겐이 없는 리물루스 아메보사이트 라이세이트를 이용한 내독소 검출방법 |
| KR20190127655A (ko) | 2016-10-19 | 2019-11-13 | 프로디자인 소닉스, 인크. | 음향학에 의한 친화성 세포 추출 |
| EP3552021B1 (en) * | 2017-01-11 | 2022-10-26 | Lonza Walkersville, Inc. | Coagulogen-free clarified limulus amebocyte lysate |
| EP3725092A4 (en) | 2017-12-14 | 2021-09-22 | FloDesign Sonics, Inc. | DRIVE AND CONTROL UNIT FOR ACOUSTIC CONVERTER |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4322217A (en) | 1980-06-27 | 1982-03-30 | Mallinckrodt, Inc. | Process for preparing Limulus lysate |
| ES2098256T3 (es) * | 1989-10-30 | 1997-05-01 | Biowhittaker Inc | Ensayo cinetico para la endotoxina utilizando lisado amebocitico de limulus y sustrato cromogenico. |
| JP3122454B2 (ja) | 1990-09-27 | 2001-01-09 | 生化学工業株式会社 | カブトガニ・アメボサイト・ライセートの調製法 |
| US5208148A (en) | 1990-12-07 | 1993-05-04 | Molecular Probes, Inc. | Lipophilic fluorescent glycosidase substrates |
| US5242805A (en) | 1991-08-23 | 1993-09-07 | Molecular Probes, Inc. | Long wavelength lipophilic fluorogenic glycosidase substrates |
| ATE180018T1 (de) | 1991-08-23 | 1999-05-15 | Molecular Probes Inc | Verwendung von haloalkylderivaten von reportermolekülen zur analyse der metabolischen aktivität in zellen |
| US5576424A (en) | 1991-08-23 | 1996-11-19 | Molecular Probes, Inc. | Haloalkyl derivatives of reporter molecules used to analyze metabolic activity in cells |
| JP3242733B2 (ja) | 1993-02-26 | 2001-12-25 | 生化学工業株式会社 | エンドトキシン特異的測定剤 |
| US5830912A (en) | 1996-11-15 | 1998-11-03 | Molecular Probes, Inc. | Derivatives of 6,8-difluoro-7-hydroxycoumarin |
| US5773236A (en) | 1997-04-25 | 1998-06-30 | Molecule Probes, Inc. | Assay for glutathiane transferase using polyhaloaryl-substituted reporter molecules |
| CA2684578A1 (en) | 2007-03-21 | 2008-09-25 | Id Biomedical Corporation Of Quebec | Chimeric antigens |
| JP5403516B2 (ja) | 2007-11-12 | 2014-01-29 | 国立大学法人広島大学 | エンドトキシンの濃度測定方法および濃度測定用キット |
| US7846678B2 (en) | 2008-08-18 | 2010-12-07 | BioDtech, Inc. | Enhancing endotoxin detection |
| MY155541A (en) | 2010-10-29 | 2015-10-30 | Universiti Malaysia Terengganu | Process for the preparation of amoebocyte lysate from the haemolymph of the horseshoe crab |
| CN102305788A (zh) | 2010-11-22 | 2012-01-04 | 天津市一瑞生物工程有限公司 | 革兰阴性菌脂多糖(内毒素)比色检测试剂盒的制备及其使用方法 |
| KR102561368B1 (ko) | 2016-08-03 | 2023-08-01 | 론자 워커스빌 아이엔씨. | 실질적으로 코아굴로겐이 없는 리물루스 아메보사이트 라이세이트를 이용한 내독소 검출방법 |
| EP3552021B1 (en) | 2017-01-11 | 2022-10-26 | Lonza Walkersville, Inc. | Coagulogen-free clarified limulus amebocyte lysate |
| WO2019006000A1 (en) * | 2017-06-28 | 2019-01-03 | Lonza Walkersville, Inc. | CARTRIDGE FOR THE DETECTION OF ENDOTOXINS |
-
2017
- 2017-08-02 KR KR1020197005917A patent/KR102561368B1/ko active Active
- 2017-08-02 JP JP2019504062A patent/JP7044222B2/ja active Active
- 2017-08-02 WO PCT/US2017/045137 patent/WO2018026941A1/en not_active Ceased
- 2017-08-02 CN CN201780048853.4A patent/CN109790561B/zh active Active
- 2017-08-02 IL IL264464A patent/IL264464B2/en unknown
- 2017-08-02 EP EP17837616.6A patent/EP3494226B1/en active Active
- 2017-08-02 EP EP21183657.2A patent/EP3943608B1/en active Active
- 2017-08-02 SG SG11201900767VA patent/SG11201900767VA/en unknown
- 2017-08-03 US US15/668,101 patent/US11156614B2/en active Active
-
2021
- 2021-09-23 US US17/482,918 patent/US11906521B2/en active Active
Non-Patent Citations (2)
| Title |
|---|
| Biochim Biophys Acta, 707(2): 217-225 (1982.10.05.)* |
| Chem Biol Interact, 249: 19-22 (2016.02.23.)* |
Also Published As
| Publication number | Publication date |
|---|---|
| EP3494226A1 (en) | 2019-06-12 |
| IL264464B1 (en) | 2023-10-01 |
| WO2018026941A1 (en) | 2018-02-08 |
| CN109790561B (zh) | 2024-02-06 |
| EP3943608B1 (en) | 2023-07-12 |
| US11156614B2 (en) | 2021-10-26 |
| US20220074940A1 (en) | 2022-03-10 |
| EP3494226B1 (en) | 2021-09-22 |
| US20180038864A1 (en) | 2018-02-08 |
| EP3494226A4 (en) | 2020-03-18 |
| JP7044222B2 (ja) | 2022-03-30 |
| EP3943608A1 (en) | 2022-01-26 |
| IL264464A (en) | 2019-02-28 |
| IL264464B2 (en) | 2024-02-01 |
| SG11201900767VA (en) | 2019-02-27 |
| CN109790561A (zh) | 2019-05-21 |
| JP2019526052A (ja) | 2019-09-12 |
| US11906521B2 (en) | 2024-02-20 |
| KR20190037284A (ko) | 2019-04-05 |
| EP3943608C0 (en) | 2023-07-12 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR102561368B1 (ko) | 실질적으로 코아굴로겐이 없는 리물루스 아메보사이트 라이세이트를 이용한 내독소 검출방법 | |
| JP7724762B2 (ja) | コアギュローゲンを含まない、清澄化リムルスアメボサイトライセート | |
| WO2014092079A1 (ja) | 新規組換えファクターｃ、その製造法、およびエンドトキシンの測定法 | |
| JP2012068266A (ja) | 生体サンプル中の微生物を分類する方法 | |
| Sheraba et al. | Advanced approaches for endotoxin detection and removal from snake antivenoms | |
| US20090317838A1 (en) | Detection of bacterial peptidoglycan-like compounds | |
| Hodes et al. | Reaction of fungal products with amebocyte lysates of the Japanese horseshoe crab, Tachypleus tridentatus | |
| HK40006346B (zh) | 利用基本上不含凝固蛋白原的鲎阿米巴样细胞溶解物检测内毒素的方法 | |
| HK40006346A (en) | Method of detecting an endotoxin using limulus amebocyte lysate substantially free of coagulogen | |
| Vanhaecke et al. | Endotoxin testing | |
| CN104163869B (zh) | 重组细菌辨识蛋白及其用途 | |
| HK40007693B (en) | Coagulogen-free clarified limulus amebocyte lysate | |
| HK40007693A (en) | Coagulogen-free clarified limulus amebocyte lysate | |
| Williams | Limulus amebocyte lysate discovery, mechanism, and application | |
| Friberger | Endotoxin determination with Limulus lysate in clinical diagnosis and hygienic control | |
| Berzofsky | Endotoxin, Limulus Amebocyte Lysate, and Filter Applications |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
|
| P13-X000 | Application amended |
St.27 status event code: A-2-2-P10-P13-nap-X000 |
|
| PA0105 | International application |
St.27 status event code: A-0-1-A10-A15-nap-PA0105 |
|
| PG1501 | Laying open of application |
St.27 status event code: A-1-1-Q10-Q12-nap-PG1501 |
|
| AMND | Amendment | ||
| E13-X000 | Pre-grant limitation requested |
St.27 status event code: A-2-3-E10-E13-lim-X000 |
|
| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
|
| P13-X000 | Application amended |
St.27 status event code: A-2-2-P10-P13-nap-X000 |
|
| A201 | Request for examination | ||
| PA0201 | Request for examination |
St.27 status event code: A-1-2-D10-D11-exm-PA0201 |
|
| E902 | Notification of reason for refusal | ||
| PE0902 | Notice of grounds for rejection |
St.27 status event code: A-1-2-D10-D21-exm-PE0902 |
|
| AMND | Amendment | ||
| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
|
| P13-X000 | Application amended |
St.27 status event code: A-2-2-P10-P13-nap-X000 |
|
| E601 | Decision to refuse application | ||
| PE0601 | Decision on rejection of patent |
St.27 status event code: N-2-6-B10-B15-exm-PE0601 |
|
| AMND | Amendment | ||
| E13-X000 | Pre-grant limitation requested |
St.27 status event code: A-2-3-E10-E13-lim-X000 |
|
| P11-X000 | Amendment of application requested |
St.27 status event code: A-2-2-P10-P11-nap-X000 |
|
| P13-X000 | Application amended |
St.27 status event code: A-2-2-P10-P13-nap-X000 |
|
| PX0901 | Re-examination |
St.27 status event code: A-2-3-E10-E12-rex-PX0901 |
|
| PX0701 | Decision of registration after re-examination |
St.27 status event code: A-3-4-F10-F13-rex-PX0701 |
|
| X701 | Decision to grant (after re-examination) | ||
| GRNT | Written decision to grant | ||
| PR0701 | Registration of establishment |
St.27 status event code: A-2-4-F10-F11-exm-PR0701 |
|
| PR1002 | Payment of registration fee |
St.27 status event code: A-2-2-U10-U12-oth-PR1002 Fee payment year number: 1 |
|
| PG1601 | Publication of registration |
St.27 status event code: A-4-4-Q10-Q13-nap-PG1601 |