KR102552608B1 - Feed composition for producing functional eggs containing sulforaphane and method for producing eggs using the same - Google Patents

Abstract

The present invention relates to a feed composition for producing functional eggs containing sulforaphane and a method for producing functional eggs using the same. Anticancer effect, inhibition of activity of inflammatory factors, inhibition of activation of glial cells, reduction of neuropathic pain when ingested, inhibition of fat accumulation, differentiation into fat cells through feeding of a feed characterized in that it comprises Obesity prevention or treatment through inhibition of expression of PPARγ, an important transcription factor expressed at the time of birth This makes it possible to contribute to increasing the income of poultry farms.

Description

Feed composition for producing functional eggs containing sulforaphane and method for producing eggs using the same {Feed composition for producing functional eggs containing sulforaphane and method for producing eggs using the same}

The present invention inhibits genetic modification caused by oxidative damage by inducing the expression of antioxidant enzymes, inhibits the growth of Helicobacter pylori, known as one of the causes of gastric cancer, and inhibits the generation of gastric cancer by carcinogens in animal tests. As a result, the effect of inhibiting the activity of inflammatory factors, inhibiting the activation of glial cells, reducing neuropathic pain when ingested, inhibiting fat accumulation, and inhibiting the expression of PPARγ, an important transcription factor expressed during differentiation into adipocytes It relates to a feed composition for producing functional eggs containing sulforaphane that effectively inhibits muscle atrophy by effectively suppressing muscle atrophy without being toxic to obesity prevention or treatment, cells, and a method for producing functional eggs using the same .

Eggs are generally known as a complete food, and have low energy compared to their high nutritional value, are well digested and absorbed, and are relatively inexpensive, so they are widely used in nutritional foods.

The nutritional composition of egg white and egg yolk, which constitute eggs, are very different, and when looking at the overall nutritional composition, they contain abundant nutritional values in general components, fatty acids, minerals, and amino acids. Egg white contains 90% of water, the rest of the protein, and a small amount of carbohydrates, and is characterized by being fat-free. In contrast, egg yolk, which accounts for 1/3 of the total weight, contains fat, so it is high in calories and cholesterol. . Egg yolk protein is also contained as much as egg white, and most of the vitamins and minerals of eggs are also contained in egg yolk. Carbohydrates are contained in egg white in small amounts, around 1%. Of the protein and vitamin B groups, niacin and riboflavin are found in egg whites and egg yolks in about half.

Recently, as consumers' health-oriented consciousness has increased, the implementation of specific ingredients in feed related to eggs (Park et al. 2005, Kor J Poult Sci 32(4), 269-273; Kim et al. 2002, J Kor Soc Food Sci Nutr 31, 373-377; Kang et al. 1994, Kor J Poult Sci 21, 227-237), and Korean Patent Publication No. 10-2018-0111141 discloses corn, wheat, soybean meal, lupine, A method is disclosed in which the ratio of omega-3 fats to omega-6 fats is less than 1:4.5 using a feed containing flax, limestone, potassium phosphate, salt, methionine, choline chloride, vitamins, minerals and probiotics.

Based on these studies, a feed capable of producing fertilized eggs with increased sulforaphane content and a fertilized egg production method using the feed were completed, leading to the present invention.

The sulforaphane has a chemical formula of C ₆ H ₁₁ NOS _{2 ,} a molecular weight of 177.29, and a chemical structure of isothiocyanate, and has a strong antioxidant effect by researchers at Johns Hopkins Medical School in the United States in 1992 this is known. Broccoli extract containing sulforaphane inhibits genetic modification caused by oxidative damage by inducing the expression of antioxidant enzymes, inhibits the growth of Helicobacter pylori, known as one of the causes of gastric cancer, and suppresses gastric cancer caused by carcinogens in animal tests. results in inhibition of production.

In addition to the above, sulforaphane inhibits the activity of inflammatory factors, inhibits the activation of glial cells, reduces neuropathic pain when ingested, inhibits fat accumulation, and has PPARγ, an important transcription factor expressed during differentiation into adipocytes. It has been reported that it prevents or treats obesity through the expression inhibition effect, and prevents, treats, or improves muscle atrophy by effectively inhibiting muscle atrophy without being toxic to cells.

1. Republic of Korea Patent Publication No. 10-2018-0111141 "Omega Balance Egg Feed Composition" 2. Patent Application No. 10-2012-0041948 “Health functional food composition containing sulforaphane and analogues thereof” 3. Patent Application No. 10-2012-0022381 “Pharmaceutical composition for preventing or treating obesity containing sulforaphane or sulforaphane precursor or derivative” 4. Patent Application No. 10-2018-0112948 “Composition for preventing, treating, or improving muscle atrophy and muscle loss containing DL-sulforaphane N-acetyl-L-cysteine”

Therefore, the technical problem to be achieved by the present invention is to provide a feed capable of producing functional eggs containing sulforaphane and a method for producing sulforaphane-containing functional eggs using the feed.

In order to achieve the above object to be solved, the present invention is characterized in that it contains 3 to 15% of any one or more dry pulverized material selected from the group consisting of chicory, gondola and chrysanthemum by weight For producing functional eggs containing sulforaphane A feed composition is provided.

In the feed composition according to the present invention, the dry pulverized product is characterized in that it is performed after performing a water bath for 30 minutes to 100 minutes in purified water at 20 ° C to 60 ° C, and then dried.

In the feed composition according to the present invention, the water bath is characterized in that it is carried out after adding 5 to 15 parts by weight of aronia dry powder based on 100 parts by weight of the dry pulverized material.

In the feed composition according to the present invention, the dry pulverized product is characterized in that the ground part is dried and pulverized.

In addition, the present invention provides a method for producing sulforaphane-containing functional eggs, characterized in that the feed as described above is used, and sulforaphane-containing functional eggs produced by the production method.

It is preferable that the sulforaphane-containing functional eggs produced according to the present invention are fertilized eggs.

When the feed composition according to the present invention is ingested, it has anticancer effect, inhibits the activity of inflammation-inducing factors, inhibits the activation of glial cells, reduces neuropathic pain when ingested, inhibits fat accumulation, and differentiates into fat cells. Prevention or treatment of obesity through the expression inhibitory effect of PPARγ, an important transcription factor expressed, production of functional eggs containing sulforaphane that has the effect of preventing, treating or improving muscle atrophy by effectively inhibiting muscle atrophy without being toxic to cells. This can contribute to increasing the income of poultry farms.

Hereinafter, specific details for carrying out the present invention will be described in detail.

In order to achieve the object to be solved, the present invention is a functional egg containing sulforaphane, characterized in that it contains 3 to 15% of any one or more dry pulverized material selected from the group consisting of chicory, gondola and crown daisy by weight. It provides a feed composition for production.

Although there is no particular limitation, in the present invention, it is preferable to use all of the above-ground parts except for the roots of chicory, gondola and crown daisy, and the content is 3 to 15% by weight based on the total feed composition, and the content is 3 to 15% by weight If it is less than 3% by weight, the desired effect cannot be obtained in the present invention, and if it exceeds 15% by weight, it may adversely affect the egg weight and egg laying rate.

In addition, in the present invention, in order to increase the transfer rate of sulforaphane, chicory, gondola, and crown daisy are dried after bathing, and the bathing is performed in purified water at 20 ° C to 60 ° C for 30 to 100 minutes. .

In addition, in order to further enhance the transition effect, it is preferable to further include aronia dry powder in the purified water to be heated, followed by hot water, and the aronia dry powder is 100 parts by weight of any one or more pulverized materials selected from the group consisting of chicory, gondola and crown daisy It is characterized in that it is carried out after adding 5 to 15 parts by weight of aronia dry powder.

The feed feeding method for producing sulforaphane-containing functional eggs through feeding of feed according to the present invention as described above is not particularly limited as long as it is a method widely known in the art to which the present invention belongs, and the functional eggs produced according to the present invention are It is preferable that it is an fertilized egg.

The fertilized egg is a high-quality food in terms of nutrition and is a well-balanced food containing rich nutrients such as unsaturated fatty acids, iron, vitamins A, D, E. K and B, including high-quality protein (Forsyhe 1970, The Bakers Digest 8, 4-46).

The present invention will be described in more detail through the following examples and test examples.

1-1. Laying hens and breeding methods

120 48-week-old 'High Brown' (20 each in 5 test groups, 20 in control group) were announced in fertilized egg production farms and reared for 6 weeks. Breeding was carried out in 3 repetitions, with 10 hens and 1 rooster per repetition, and they were reared in cages with an area of 18 m ² , and the weights were similarly distributed.

1-2. feed and salary

Feed was fed at 11:00 am at 150 g per animal, and the feed was as follows.

Control feed: 15% corn feed, 25% rice and barley bran, 10% wheat bran, 10% tofu okara, 20% ocher, 20% enoki mushroom medium (weight ratio)

Feed for test group 1: 10% corn feed, 20% rice and barley bran, 10% wheat bran, 10% tofu okara, 20% ocher, 20% enoki mushroom medium. 10% (weight ratio) of pulverized product prepared by drying the above ground part of chicory and pulverizing it with 60-80 mesh

Feed for test group 2: 10% corn feed, 20% rice and barley bran, 10% wheat bran, 10% tofu okara, 20% ocher, 20% enoki mushroom medium. 10% (by weight) of pulverized product prepared by drying the above ground part of chicory in hot water and then pulverizing it with 60-80 mesh

Feed for test group 3: 10% corn feed, 20% rice and barley bran, 10% wheat bran, 10% tofu okara, 20% ocher, 20% enoki mushroom medium. 10% (weight ratio) of pulverized product prepared by drying the above ground part of chicory (containing aronia dry powder) and pulverizing with 60-80 mesh

Test group 4 feed: 10% corn feed, 20% rice and barley bran, 10% wheat bran, 10% tofu okara, 20% ocher, 20% enoki mushroom medium. 10% (weight ratio) of pulverized product prepared by drying the aerial parts of gondalbi and pulverizing them with 60-80 mesh

Feed for test group 5: corn feed 10%, rice and barley bran 20%, wheat bran 10%, tofu okara 10%, ocher 20%, enoki mushroom medium 20%. 10% (by weight) of pulverized product prepared by drying the ground part of Gondalbi in hot water and then pulverizing it with 60-80 mesh

Feed for test group 6: 10% corn feed, 20% rice and barley bran, 10% wheat bran, 10% tofu okara, 20% ocher, 20% enoki mushroom medium. 10% (weight ratio) of pulverized product prepared by drying the aerial part of Gondalbi after double-boiling (containing aronia dry powder) and pulverizing with 60-80 mesh

Feed for test group 7: 10% corn feed, 20% rice and barley bran, 10% wheat bran, 10% tofu okara, 20% ocher, 20% enoki mushroom medium. 10% (weight ratio) of pulverized product prepared by drying the aerial part of crown daisy and then pulverizing it with 60-80 mesh

Feed for test group 8: corn feed 10%, rice and barley bran 20%, wheat bran 10%, tofu okara 10%, ocher 20%, enoki mushroom medium 20%. 10% (by weight) of pulverized product prepared by drying the above-ground part of crown daisy in hot water and then pulverizing it with 60-80 mesh

Feed for test group 9: 10% corn feed, 20% rice and barley bran, 10% wheat bran, 10% tofu okara, 20% ocher, 20% enoki mushroom medium. 10% (weight ratio) of pulverized product prepared by drying the above-ground parts of crown daisy (containing aronia dry powder) and then pulverizing with 60-80 mesh

1-3. spawning rate

In the case of day 0, the egg laying rate was the average value of the week before feeding, and for the 3rd day of feeding, it was 3 days from the day the feeding was started. It was expressed as the number of eggs per average number per day.

1-4. weight of fertilized eggs

The weight of fertilized eggs was the average weight of fertilized eggs laid per day.

1-5. of fertilized eggs sulforaphane content

* standard solution

Standard stock solution - DL-Sulforaphane, 5mg

- Product number: S4441-5MG , Sigma

* HPLC analysis conditions

item
condition
HPLC
Dionex Ultimate 3000
column
Thermo Acclaim C18, 4.6*250mm, 5um
detector
UV 202nm
mobile phase
(run time : 20min)
A: Acetonitrile/DW (30:70)
No.
Time(min)
A (%)
B (%)
One
0
100
0
2
28
100
0
inlet volume
10 µL
flow rate
1.0 mL/min
column temperature
40℃

* sample

Ultrasonic extraction of 10g of egg yolk with 30mL of methanol for 15 minutes, centrifugation to take the supernatant (repeated twice), 10g of NaCl was added thereto, shaking for 10min, and centrifugation to separate only the MeOH layer for use

1-6. data analysis

The data analysis of the present invention was performed more than 3 times each, and the significance between treatments was tested by Duncan's multiple test using SPSS (Statistics Program for Social Science) 12.0.

2-1. spawning rate

The egg production rate of the laying hens was measured and shown in Table 2, and the egg production rate was insignificantly increased in the test group compared to the control group. Also, there was no difference within the test group.

spawning rate
(%) Feed feeding period (days) 0 3 7 14 21 28 35 control group 70 ^az 70 a 70 a 70 a 70 a 70 a 70 a test group 1 70 a 72a 72a 72a 72a 72a 72a test group 2 70 a 72a 72a 72a 72a 72a 72a test group 3 70 a 71a 72a 72a 72a 72a 72a test group 4 71a 71a 72a 72a 72a 72a 72a test group 5 70 a 72a 73a 73a 73a 73a 73a test group 6 70 a 72a 72a 72a 73a 72a 72a test group 7 70 a 72a 72a 72a 73a 72a 73a test group 8 70 a 72a 72a 73a 72a 73a 72a Trial group 9 70 a 72a 72a 73a 72a 72a 73a

^z Mean separation within rows by Duncan's multiple range test at 5% level.

2-2. weight of fertilized eggs

The weight of fertilized eggs was measured and shown in Table 3. In the test group and the control group, the weight tended to increase after administration. , there was no difference within the test group.

fertilized eggs
weight (g) Feed feeding period (days) 0 3 7 14 21 28 35 control group 54.1 ^az 55.8 a 61.5 a 61.8 a 61.8 a 62.1 a 60.5 a test group 1 54.0 a 57.8 a 62.4 a 62.8 a 63.01a 62.4 a 62.5 a test group 2 54.1a 57.8 a 62.4 a 62.7 a 63.01a 62.4 a 62.5 a test group 3 54.0 a 57.7 a 62.5 a 62.8 a 63.01a 62.4 a 62.5 a test group 4 54.0 a 57.8 a 62.4 a 62.9 a 63.01a 62.4 a 62.5 a test group 5 54.1a 58.8 a 62.5 a 62.7 a 63.03a 62.4 a 62.9 a test group 6 54.1a 58.8 a 62.4 a 61.8 a 63.01a 62.4 a 62.5 a test group 7 54.1a 57.8 a 61.5 a 62.7 a 63.01a 62.5 a 62.5 a test group 8 54.0 a 57.8 a 62.4 a 62.7 a 63.01a 62.9 a 62.5 a Trial group 9 54.0 a 58.8 a 62.4 a 62.8 a 63.01a 62.9 a 62.5 a

^z Mean separation within rows by Duncan's multiple range test at 5% level.

2-4. sulforaphane content

The content of sulforaphane contained in egg yolk was analyzed and the results are shown in Table 4.

content
(mg/g egg yolk) Feed feeding period (days) 0 3 7 14 21 28 35 control group 1.5 ^az 1.5a 1.5a 1.6a 1.6a 1.6a 1.6a test group 1 1.5a 7.7a 14.3a 16.2a 16.5a 16.8a 16.9a test group 2 1.5a 8.8a 16.1a 20.4 a 21.1a 21.2a 20.9a test group 3 1.5a 10.1a 18.5a 24.7 a 24.8 a 25.1 a 25.2 a test group 4 1.5a 7.6 a 14.4a 16.4a 16.1a 17.2a 17.2a test group 5 1.5a 8.7a 16.2a 19.9a 20.9a 21.4 a 21.8 a test group 6 1.5 ^az 9.9a 19.2a 24.8 a 25.5 a 25.4 a 25.7 a test group 7 1.5 ^az 7.1a 14.2a 16.7a 16.5a 17.1a 16.9a test group 8 1.5 ^az 9.1a 17.2a 20.5 a 21,5 a 22.1a 22.2 a Trial group 9 1.5 ^az 10.4a 19.8a 24.7 a 25.7 a 26.2 a 26.2 a

^z Mean separation within rows by Duncan's multiple range test at 5% level.

Referring to Table 5, it was confirmed that the sulforaphane content increased significantly in the test group compared to the control group.

As described above, it can be confirmed that the sulforaphane content in the feed composition of the present invention is significantly increased, and thus the usefulness of the present invention has been proven.

Therefore, by inducing the expression of antioxidant enzymes, genetic modification caused by oxidative damage is suppressed, the growth of Helicobacter pylori known as one of the causes of gastric cancer is inhibited, and the formation of gastric cancer by carcinogens is inhibited in animal tests. , Inhibiting the activity of inflammatory factors, inhibiting the activation of glial cells, reducing neuropathic pain when ingested, inhibiting fat accumulation, and inhibiting the expression of PPARγ, an important transcription factor expressed during differentiation into adipocytes. It is possible to provide a feed composition for producing functional eggs containing sulforaphane that effectively inhibits muscle atrophy by effectively suppressing muscle atrophy without being toxic to the cells, preventing or treating obesity through the use of sulforaphane, and a method for producing functional eggs using the same. do.

Claims (7)

A feed composition for producing functional eggs containing sulforaphane, characterized in that it contains 10% by weight of dry pulverized aerial parts of gondola.
The feed composition for producing sulforaphane-containing functional eggs according to claim 1, wherein the pulverized product is dried after being heated in purified water at 20 ° C to 60 ° C for 30 minutes to 100 minutes.
The feed composition for producing sulforaphane-containing functional eggs according to claim 2, wherein the water bath is performed after adding 5 to 15 parts by weight of aronia dry powder based on 100 parts by weight of the pulverized material.
A method for producing sulforaphane-containing functional eggs, characterized by feeding laying hens with a feed composition containing 10% by weight of dry pulverized material of the aerial part of gondola.

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